SDD-AGEとは 意味・読み方・使い方

SDD-AGE is short for Semi-Denaturating Detergent Agarose Gel Electrophoresis. This is a method for detecting and characterizing large protein polymers which are stable in 2% SDS at room temperature, unlike most large protein complexes. This method is very useful for studying prions and amyloids, which are characterized by the formation of proteinaceous polymers. Agarose is used for the gel since the SDS-resistant polymers are large (in the 200-4000+ КDa range) and cannot enter a conventional polyacrylamide gel, which has small pores. Agarose on the other hand has large pores, which allows for the separation of polymers.