NZ613707B2 - Purine Derivatives and Pharmaceutically Acceptable salts thereof - Google Patents

Abstract

Disclosed are purine and pyrrolo[2,3-d]pyrimidine compounds of formula (I) in which all of the variables are as defined herein. Examples are 8-(1H-indol-4-yl)-2,6-bis-((R)-3-methyl-morpholin-4-yl)-9H-purine and 8-(1H-indol-4-yl)-6-((R)-3-methyl-morpholin-4-yl)-2-(8-oxa-3-azabicyclo[3.2.1]oct-3-yl)-9H-purine. Also disclosed are pharmaceutical compositions comprising these compounds and their use as inhibitors of PI3K protein kinases and mTOR, therefore the compounds are useful in the treatment of conditions such as cancer and neurodegenerative disorders. H-purine. Also disclosed are pharmaceutical compositions comprising these compounds and their use as inhibitors of PI3K protein kinases and mTOR, therefore the compounds are useful in the treatment of conditions such as cancer and neurodegenerative disorders.

Description

Purine Derivatives and Pharmaceutically Acceptable salts thereof Field of the Invention The invention relates to purine derivatives and pharmaceutically acceptable salts thereof, ses for their preparation, their use in the treatment of diseases, their use, either alone or in combination with at least one onal therapeutic agent and optionally in combination with a pharmaceutically acceptable carrier, for the manufacture of pharmaceutical preparations, use of the pharmaceutical preparations for the treatment of diseases, and a method of ent of said diseases, comprising administering the purine derivatives to a warm-blooded animal, especially a human.

Background of the Invention The phosphatidylinositolkinases superfamily comprises 4 different PI3K related lipid or protein kinases. Class I, II and III are lipid kinases that differ by virtue of their substrate specificities whereas class IV PI3Ks (also called PIKKs) are protein kinases. Class I phosphatidylinositolkinases comprise a family of lipid kinases that catalyze the transfer of phosphate to the D-3' position of inositol lipids to produce phosphoinositolphosphate (PIP), phosphoinositol-3,4-diphosphate (PIP2) and phosphoinositol-3,4,5-triphosphate (PIP 3) that, in turn, act as second messengers in signaling cascades by docking proteins containing pleckstrin-homology, FYVE, Phox and other phospholipid-binding domains into a variety of signaling complexes often at the plasma membrane ((Vanhaesebroeck et al., Annu. Rev.

Biochem 70:535 (2001); Katso et al., Annu. Rev. Cell Dev. Biol. 17:615 (2001)). Of the two Class I PI3Ks, Class IA PI3Ks are heterodimers composed of a tic p110 subunit (? ?, ?, rms) constitutively associated with a regulatory subunit that can be p85?, p55?, p50?, p85 ? or p55?. The Class IB sub-class has one family member, a heterodimer composed of a catalytic p110? subunit associated with one of two regulatory subunits, p101 or p84 n et al., Annu Rev. Biochem. 67:481 ; Suire et al., Curr. Biol. 15:566 (2005)). The modular s of the p85/55/50 subunits include Src Homology (SH2) domains that bind phosphotyrosine residues in a specific ce context on activated receptor and cytoplasmic tyrosine kinases, ing in tion and localization of Class IA PI3Ks. Class IB PI3K is activated directly by G protein-coupled receptors that bind a diverse repertoire of peptide and non-peptide ligands (Stephens et al., Cell 89:105 (1997)); Katso et al., Annu. Rev.

Cell Dev. Biol. 17:615-675 (2001)). Consequently, the resultant phospholipid products of class I PI3K link upstream receptors with downstream ar activities including proliferation, survival, chemotaxis, cellular trafficking, ty, lism, inflammatory and allergic responses, transcription and translation ey et al., Cell 64:281 (1991); Escobedo and Williams, Nature 335:85 ; Fantl et al., Cell 69:413 (1992)).

In many cases, P|P2 and P|P3 recruit Akt, the product of the human homologue of the viral oncogene v-Akt, to the plasma membrane where it acts as a nodal point for many intracellular signaling pathways ant for growth and al (Fantl et al., Cell 69:413- 423(1992); Bader et al., Nature Rev. Cancer 5:921 (2005); Vivanco and Sawyer, Nature Rev.

Cancer 2:489 (2002)). Aberrant regulation of PI3K, which often increases al through Akt activation, is one of the most prevalent events in human cancer and has been shown to occur at multiple levels. The tumor suppressor gene PTEN, which dephosphorylates oinositides at the 3' position of the inositol ring and in so doing nizes PI3K activity, is functionally deleted in a variety of tumors. In other tumors, the genes for the p1100i isoform, , and for Aktare amplified and increased protein expression of their gene products has been demonstrated in several human cancers. Furthermore, mutations and translocation of p850 that serve to up-regulate the p85-p110 complex have been described in human cancers. Also, somatic missense ons in PIK3CA that activate downstream signaling pathways have been described at significant ncies in a wide diversity of human cancers (Kang at al., Proc. Natl. Acad. Sci. USA 102:802 (2005); Samuels et al., e 4 (2004); s et al., Cancer Cell 7:561-573 (2005)). These observations show that deregulation of phosphoinositoI-3 kinase and the upstream and downstream components of this signaling pathway is one of the most common deregulations ated with human cancers and proliferative diseases (Parsons et al., Nature 436:792 (2005); Hennessey at e|., Nature Rev. Drug Disc. 4:988—1004 (2005)).

The mammalian target of rapamycin (mTOR) is a member of the class IV PI3K. mTOR assembles a signaling network that transduces nutrient signals and various other stimuli to regulate a wide range of cellular ons including cell growth, proliferation, survival, autophagy, s types of differentiation and metabolism. In mammalian cells, the mTOR protein is found complexed in two ct entities called mTORC1 and mTORC2.

The mTORC1 complex, that is to say mTOR associated with raptor, has been the matter of numerous studies. It is mTORC1 that integrates nutrient and growth factor inputs, and is in turn responsible for cell growth regulation, mainly through protein synthesis tors such as 4EBP1 or RPS6. mTORC1 regulation requires P|3K and Akt activation for activation, meaning that mTORC1 is an effector of the P|3K pathway. mTOR when associated in the mTOR complex 2 (mTORC2) has been shown to be responsible for the activation of Akt by phosphorylation of S473 (Akt 1 numbering) (Sarbassov et al., Science 307:7098 (2005)). mTORC2 is hence here considered as an upstream activator of Akt. Interestingly mTOR can therefore be considered as being important both upstream and downstream of Akt. mTOR catalytic ton might therefore represent a unique way of addressing a very strong block in the Pl3K-Akt pathway, by addressing both upstream and downstream effectors.

A link between mTOR inhibition and autophagy has also been demonstrated (Ravikumar et a|., Nat Genet. 585-95 (2004)). Autophagy is ial for neuronal homeostasis and its dysfunction has been linked to neurodegeneration. Loss of autophagy in neurons causes neurodegenerative disease in mice (Komatsu et a|., Nature 441 :880-4 (2006); Hara et a|., Nature 441:885-9 (2006)) suggesting a critical role for autophagy to maintain protein homeostasis in neurons. Neurodegenerative es are characterized by inclusions of misfolded proteins as one of the hallmarks. Induction of autophagy enhances nce of misfolded proteins and thus is proposed as therapy for neurodegenerative proteinopathies.

Huntington‘s Disease (HD) is an autosomal dominant neurodegenerative disorder where a mutation of IT15 gene ng the Huntingtin (Htt) protein leads to Polyglutamine expansion in Exon1 of Htt. Intracellular aggregation of this mutant Htt protein and brain atrophy (in particular cortex and striatum) are the main hallmarks of HD. It clinically leads to movement bance and cognitive dysfunction besides psychiatric disturbances and weight loss. tion of mTOR induces autophagy and reduces mutant Htt aggregation and mutant Htt- mediated cell death in in vitro and in vivo models of HD (Ravikumar et a|., Nat Genet. 36(6):585-95 (2004)). mTOR inhibition therefore provides an opportunity for pharmaceutical intervention and modulation of the disrupted cellular processes characteristic of HD.

In view of the above, inhibitors of class I P|3Ks and mTOR are considered to be of value in the treatment of proliferative diseases and other disorders, in particular, HD.

The present invention relates to novel purine tives having class I PI3K and/or mTOR inhibitory activity, their preparation, medical use and to medicaments comprising them. y of the Invention In a first aspect, the ion relates to a compound of a (I), or a pharmaceutically acceptable salt thereof, 2012/050428 wherein X represents N or CH; R1 represents wherein R18 and R22 independently represent hydrogen, halogen, hydroxy or hydroxy-C1_3alkyl-; R19 and R21 independently represent hydrogen, amino, hydroxy, carboxy, C1_3a|koxy, amino-C1_3alkyl-, C1_3alkyl-C(=O)-NH-, C1_3alkyl-S(=O)m-NH- or hydroxy-C1_3alkyl-; m represents 0, 1 or 2; R20 represents hydrogen, halogen or C1_3alkoxy; or R1 is selected from the group consisting of HN\ / HN \ ZI , and wherein R23 ents hydrogen or methyl; R24 represents hydrogen, oxo or C1_3alkyl; R2, R3, R4, R5, R6, R7, R8, R9, R10, R“, R12, R13, R14, R15, R16 and R17 ndently ent hydrogen or ; or R2 and R8 together form an ethylene bridge; or R2 and R6 together form a methylene bridge; or R12 and R14 together form an ethylene bridge; and Y represents 0, CHR25 or CR26R27 wherein R25 represents hydroxy or C1-3alkoxy; and R26 and R27 independently represent hydrogen or halogen; with the proviso that the compound of formula (I) is other than 2,6-di-morpholinylphenyl- 9H-purine.

In a second aspect, the invention relates to a compound of formula (I), or a pharmaceutically acceptable salt thereof, wherein X ents N or CH; R1 represents R19 R18 wherein R18 and R22 independently represent hydrogen, halogen, hydroxy or hydroxy-C1_3alkyl-; R19 and R21 independently represent hydrogen, amino, y, carboxy, C1-3alkoxy, amino-C1_3alkyl-, C1_3alkyl-C(=O)-NH-, C1_3alkyl-S(=O)m-NH- or hydroxy-C1_3alkyl-; WO 04776 m represents 0, 1 or 2; R20 represents hydrogen, halogen or C1-3alkoxy; or R1 is selected from the group consisting of HN \ HN/ \ ZI , and wherein R23 represents en or methyl; R24 represents hydrogen, oxo or C1_3alkyl; R2, R3, R4, R5, R6, R7, R8, R9, R10, R“, R12, R13, R14, R15, R16 and R17 independently represent hydrogen or methyl; or R5 and R6 together form an ethylene bridge; and Y represents 0, CHR25 or 7 wherein R25 represents hydroxy or C1-3alkoxy; and R26 and R27 independently represent hydrogen or halogen; with the proviso that the nd of formula (I) is other than -morpholinylphenyl- 9H-purine. tions As used , the term “halogen” or “halo” refers to fluoro, chloro, bromo, and iodo.

As used herein, the term “C1-3alkyl” refers to a fully saturated branched or unbranched hydrocarbon moiety having up to 3 carbon atoms. Representative examples of C1_3alkyl include methyl, ethyl, n—propyl and iso—propyl.

As used herein, the term “C1_3alkoxy” refers to C1_3alkyl-O-, wherein C1_3alkyl is as defined herein above. Representative examples of C1_3alkoxy include methoxy, ethoxy, propoxy and 2—propoxy.

As used herein, the term “hydroxy—C1_3alkyl” refers to a C1_3alkyl group as defined herein above, wherein one of the hydrogen atoms of the C1-3alkyl group is replaced by OH.

Representative examples of hydroxy-C1_3alkyl include, but are not limited to, hydroxyl-methyl, 2—hydroxy-ethyl, 2—hydroxy-propyl and 3-hydroxy-propyl.

As used herein, the term “amino-C1_3alkyl” refers to a C1_3alkyl group as d herein above, wherein one of the en atoms of the C1-3alkyl group is replaced by a primary amino group. Representative examples of hydroxy-C1_3alkyl include, but are not d to, amino-methyl, 2—amino-ethyl, 2—amino-propyl and 3-amino-propyl.

Detailed Description of the Invention The present invention provides compounds and pharmaceutical formulations thereof that may be useful in the ent or prevention of diseases, conditions and/or disorders modulated by the inhibition of class I P|3Ks and/or mTOR.

Embodiment 1: a compound of formula (I), or a pharmaceutically acceptable salt thereof, as described hereinbefore. ment 2: a compound of formula (I), or a pharmaceutically acceptable salt thereof, as described hereinbefore. ment 3: a compound ing to Embodiment 1 or Embodiment 2, or a pharmaceutically acceptable salt thereof, n X represents N.

Embodiment 4: a compound according to Embodiment 1 or Embodiment 2, or a pharmaceutically acceptable salt thereof, wherein X represents CH.

Embodiment 5: a compound according to any one of Embodiments 1 to 4, or a pharmaceutically acceptable salt f, wherein R1 ents R21 R22 wherein R18 and R22 independently represent hydrogen, halogen, hydroxy or hydroxy-C1_3alkyl-; R19 and R21 independently ent hydrogen, amino, hydroxy, carboxy, C1-3alkoxy, amino-C1_3alkyl-, C1_3alkyl-C(=O)-NH-, kyl-S(=O)m-NH- or hydroxy—C1_3alkyl-; m represents 0, 1 or 2; and R20 represents hydrogen, n or koxy. ment 6: a compound according to Embodiment 5, or a pharmaceutically acceptable salt thereof, wherein at least one of R18, R19, R20, R21 and R22 is not hydrogen.

Embodiment 7: a compound according to any one of Embodiments 1 to 4, or a pharmaceutically acceptable salt thereof, wherein R1 is selected from the group consisting of HN\ / HN \ ZI , and wherein R23 represents hydrogen or methyl; and R24 represents hydrogen, oxo or C1_3alkyl.

Embodiment 8: a compound according to any one of Embodiments 1 to 7, or a pharmaceutically acceptable salt thereof, wherein Y represents 0; Embodiment 9: a compound according to any one of Embodiments 1 to 7, or a pharmaceutically acceptable salt f, wherein Y represents CHR26 or CR27R28.

Embodiment 10: a compound according to Embodiment 1 or Embodiment 2, which is selected from: 2,6-Dimethyl-morpholinyl)morpholinyl-9H-purinyl]-phenol; 3-(2,4-dimorpholino-7H-pyrrolo[2,3-d]pyrimidinyl)phenol; 2,6-Bis-(3-methyl-morpholinyl)—8—(1H-pyrrolo[2,3-b]pyridinyl)—9H-purine; {2-Fluoro[6-(3-methyl-morpholinyl)morpholinyl-9H-purinyl]-phenyl}-methanol; 2—(4,4-Difluoro—piperidinyl)(1H-indolyl)(3-methyl-morpholinyl)-9H-purine; -[2,6-Bis—(3-methyI-morpholinyl)—9H-purin-8—y|]—1,3-dihydro-benzoimidazoI-Z-one; {5-[2,6-Bis—(3-methyI-morpholinyl)-9H-puriny|]-2—methoxy-pheny|}-methanol; 8—(1H-Indolyl)—2-morpholinyl(8—oxaaza-bicyclo[3.2.1]octy|)—9H-purine; 2—Methoxy[6-(3-methyI-morpholinyl)morpho|inyI-9H-purin-8—y|]-benzoic acid; oro[6-(3-methyl-morpholinyl)morpho|inyI-9H-puriny|]-pheny|}-methano|; 3-(2,6-Di-morpholinyI-9H-purin-8—yl)—benzy|amine; 1-{3-[6-(3-Methy|—morph0|inyl)morpho|inyI-9H-puriny|]-pheny|}-ethano|; 2,6-Di-morpholinyl-8—(1H-pyrrolo[3,2—b]pyridinyl)—9H-purine; 8—(1H-Indolyl)—2,6-bis—(3-methyI-morpholinyl)—9H-purine; 8—(1H-Indolyl)—2,6-bis—(3-methyI-morpholinyl)—9H-purine; 1-[8—(1H-IndoIyl)(3-methy|—morpho|inyl)-9H-purinyl]—piperidino|; {3-[2,6-Bis—(3-methyI-morpholinyl)-9H-puriny|]methoxy-pheny|}-methanol; 8—(1H-Indolyl)(3-methy|—morpho|inyl)(3-methyl-morpholinyl)—9H-purine; {3-[2,6-Bis—(3-methyI-morpholinyl)-9H-puriny|]f|uoro—pheny|}-methano|; 8—(1H-Indolyl)(3-methy|—morpho|inyl)—2-morpholinyI-9H-purine; 8—(1H-Indolyl)(3-methy|—morpho|inyl)—2-morpholinyI-9H-purine; 8—(1H-Indolyl)—2,6-bis—(3-methyI-morpholinyl)—9H-purine; Indolyl)(3-methy|—morpho|inyl)—2-morpholinyI-9H-purine; 8—(1H-Indolyl)(3-methy|—morpho|inyl)—2-morpholinyI-9H-purine; Indolyl)—2—(4-methoxy-piperidiny|)morpho|inyI-9H-purine; 8—(1H-Indolyl)—2,6-di-morpholinyI-9H-purine; 8—(1H-Indazolyl)—2,6-di-morpholinyI-9H-purine; 8—(1H-Indolyl)—2,6-di-morpholinyI-9H-purine; 3-(2,6-Di-morpholinyI-9H-purinyl)—pheny|amine; N-[3-(2,6-Di-morpholinyI-9H-purinyl)—pheny|]—acetamide; 8—(2—MethyI-1H-indolyl)—2,6-di-morpholinyI-9H-purine; 3-[2—(2,6-Dimethyl-morpholinyl)morpho|iny|—9H-purin-8—yl]—pheny|amine; N-[3-(2,6-Di-morpholinyI-9H-purinyl)-pheny|]—methanesulfonamide; {2—[2-(2,6-DimethyI-morpholinyl)morpho|inyI-9H-puriny|]-pheny|}-methano|; [2-(2,6-Di-morpholinyI-9H-purinyl)-pheny|]—methano|; 3-(2,6-Di-morpholinyI-9H-purin-8—y|)-pheno|; [3-(2,6-Di-morpholinyI-9H-purinyl)-pheny|]—methano|; 2-(2,6-Di-morpholinyI-9H-purin-8—y|)-pheno|; 3-[2—(2,6-Dimethyl-morpholinyl)morpho|iny|—9H-purin-8—y|]-pheno|; 3-(2,4-dimorpholin0-7H-pyrrolo[2,3-d]pyrimidiny|)phenol; s—(3-methyI-morpholinyl)—8—(1H-pyrro|o[2,3-b]pyridinyl)—9H-purine; {2-FIuoro—5-[6-(3-methyI-morpholinyl)morpholinyI-9H-puriny|]-pheny|}-methano|; -Dif|uoro—piperidiny|)(1H-indoIyl)(3-methy|—morpholinyl)-9H-purine; -[2,6-Bis—(3-methyI-morpholinyl)—9H-purin-8—y|]—1,3-dihydro-benzoimidazoI-Z-one; {5-[2,6-Bis—(3-methyI-morpholinyl)-9H-puriny|]-2—methoxy-pheny|}-methanol; 8—(1H-Indolyl)—2-morpholinyl(8—oxaaza-bicyclo[3.2.1]octy|)—9H-purine; 2—Methoxy[6-(3-methyI-morpholinyl)morpho|inyI-9H-purin-8—y|]-benzoic acid; {4-ChIoro[6-(3-methyl-morpholinyl)morpho|inyI-9H-puriny|]-pheny|}-methano|; 3-(2,6-Di-morpholinyI-9H-purin-8—yl)—benzy|amine; 1-{3-[6-(3-Methy|—morph0|inyl)morpho|inyI-9H-puriny|]-pheny|}-ethano|; 2,6-Di-morpholinyl-8—(1H-pyrrolo[3,2—b]pyridinyl)—9H-purine; 8—(1H-Indolyl)—2,6-bis—(3-methyI-morpholinyl)—9H-purine; 8—(1H-Indolyl)—2,6-bis—(3-methyI-morpholinyl)—9H-purine; 1-[8—(1H-IndoIyl)(3-methy|—morpho|inyl)-9H-purinyl]—piperidino|; {3-[2,6-Bis—(3-methyI-morpholinyl)-9H-puriny|]methoxy-pheny|}-methanol; 8—(1H-IndoIyl)(3-methy|—morpho|inyl)((S)—3-methyl-morpholinyl)—9H-purine; {3-[2,6-Bis—(3-methyI-morpholinyl)-9H-puriny|]f|uoro—pheny|}-methano|; 8—(1H-Indolyl)(3-methy|—morpho|inyl)—2-morpholinyI-9H-purine; 8—(1H-Indolyl)(3-methy|—morpho|inyl)—2-morpholinyI-9H-purine; 8—(1H-Indolyl)—2,6-bis—(3-methyI-morpholinyl)—9H-purine; 8—(1H-Indolyl)(3-methy|—morpho|inyl)—2-morpholinyI-9H-purine; 8—(1H-Indolyl)(3-methy|—morpho|inyl)—2-morpholinyI-9H-purine; 8—(1H-Indolyl)—2—(4-methoxy-piperidiny|)morpho|inyI-9H-purine; 8—(1H-Indolyl)—2,6-di-morpholinyI-9H-purine; 8—(1H-Indazolyl)—2,6-di-morpholinyI-9H-purine; 8—(1H-Indolyl)—2,6-di-morpholinyI-9H-purine; 3-(2,6-Di-morpholinyI-9H-purinyl)—pheny|amine; N-[3-(2,6-Di-morpholinyI-9H-purinyl)—pheny|]—acetamide; 8—(2—MethyI-1H-indolyl)—2,6-di-morpholinyI-9H-purine; 3-[2—(2,6-Dimethyl-morpholinyl)morpho|iny|—9H-purin-8—yl]—pheny|amine; N-[3-(2,6-Di-morpholinyI-9H-purinyl)-pheny|]—methanesulfonamide; {2—[2-(2,6-DimethyI-morpholinyl)morpho|inyI-9H-puriny|]-pheny|}-methano|; [2-(2,6-Di-morpholinyI-9H-purinyl)-pheny|]—methanol; 3-(2,6-Di-morpholinyl-9H-purinyl)-phenol; [3-(2,6-Di-morpholinyl-9H-purinyl)-phenyl]-methanol; 2—(2,6-Di-morpholinyl-9H-purinyl)-phenol; 6-(3,3-Dimethyl-morpholinyl)(1H-indolyl)morpholinyl-9H-purine; -Dimethyl-morpholinyl)(1H-indolyl)morpholinyl-9H-purine; 8—(2,3-Dihydro-1H-indolyl)(3-methyl-morpholinyl)(3-methyl-morpholinyl)—9H- purine; -Dihydro-1H-indolyl)—2,6-bis—(3-methyl-morpholinyl)—9H-purine; 8—(1H-lndolyl)(3-methyl-morpholinyl)((R)—3-methyl-morpholinyl)—9H-purine; 8—(1H-lndolyl)(3-methyl-morpholinyl)((R)—3-methyl-morpholinyl)—9H-purine; 8—(1H-lndolyl)—2,6-bis—(3-methyl-morpholinyl)—9H-purine; 8—(1H-lndolyl)(3-methyl-morpholinyl)(8—oxaaza-bicyclo[3.2.1]octyl)—9H- purine; 8—(1H-lndolyl)—2-( 3-methyl-morpholinyl)—6-morpholinyl-9H-purine; 8—(1H-lndolyl)morpholinyl(2—oxaaza-bicyclo[2.2.1]heptyl)—9H-purine; 8—(1H-lndolyl)(3-methyl-morpholinyl)morpholinyl-9H-purine; 8—(1H-lndolyl)—2—morpholinyl(2—oxaaza-bicyclo[2.2.1]heptyl)—9H-purine; 8—(1H-lndolyl)(3-methyl-morpholinyl)(8—oxaaza-bicyclo[3.2.1]octyl)—9H- 8—(1H-lndolyl)(3-methyl-morpholinyl)(8—oxaaza-bicyclo[3.2.1]octyl)—9H- purine; 8—(1H-lndolyl)—2—morpholinyl(3-oxa-8—aza-bicyclo[3.2.1]octyl)-9H-purine; 8—(1H-lndolyl)—2—morpholinyl(3-oxa-8—aza-bicyclo[3.2.1]octyl)-9H-purine; 6-(1H-lndolyl)(3-methyl-morpholinyl)(3-methyl-morpholinyl)—7H- pyrrolo[2,3- d]pyrimidine; 6-(1H-lndolyl)-2,4-di-morpholin- 4-yl-7H-pyrrolo[2,3-d]pyrimidine; and pharmaceutically acceptable salts thereof.

Embodiment 11: a compound according to Embodiment 1 or Embodiment 2, which is selected from: 3-[2—((28,6R)—2,6-Dimethyl-morpholinyl)morpholinyl-9H-purinyl]-phenol; 3-(2,4-dimorpholino-7H-pyrrolo[2,3-d]pyrimidinyl)phenol; 2,6-Bis—((S)—3-methyl-morpholinyl)—8—(1H-pyrrolo[2,3-b]pyridinyl)-9H-purine; {2—Fluoro[6-((S)methyl-morpholinyl)morpholinyl-9H-purinyl]-phenyl}- ol; 2—(4,4-Difluoro—piperidinyl)(1H-indolyl)((S)methyl-morpholinyl)—9H-purine; -[2,6-Bis—((S)methyl-morpholinyl)—9H-purin-8—yl]—1,3-dihydro-benzoimidazol-2—one; {5-[2,6-Bis—((S)—3-methy|—morpho|inyl)—9H-puriny|]methoxy-pheny|}-methanol; 8—(1H-Indolyl)—2-morpholinyl(8—oxaaza-bicyclo[3.2.1]octy|)—9H-purine; 2-Methoxy[6-((S)methy|—morpho|inyl)morpho|iny|—9H-purin-8—y|]-benzoic acid; {4-Ch|oro[6-((S)methy|—morpho|inyl)morpho|iny|—9H-purin-8—y|]-pheny|}- methanol; 3-(2,6-Di-morpholinyI-9H-purin-8—y|)-benzy|amine; 1-{3-[6-((S)Methy|—morpho|inyl)morpho|iny|—9H-puriny|]-pheny|}-ethano| ; -morpholinyl-8—(1H-pyrrolo[3,2—b]pyridinyl)—9H-purine; 8—(1H-Indolyl)—2,6-bis—((S)—3-methy|—morpho|inyl)—9H-purine; 8—(1H-Indolyl)—2,6-bis—((R)—3-methy|—morpholinyl)-9H-purine; 1H-IndoIyl)((S)—3-methyl-morpholinyl)—9H-purin-2—y|]-piperidinol; {3-[2,6-Bis—((S)—3-methy|—morpho|inyl)—9H-puriny|]methoxy-pheny|}-methanol; 8—(1H-Indolyl)((R)—3-methyl-morpholinyl)((S)methy|—morpholinyl)—9H-purine; {3-[2,6-Bis—((R)—3-methyI-morpholinyl)—9H-puriny|]f|uoro—pheny|}-methano|; 8—(1H-Indolyl)((R)—3-methy|—morpholinyl)morpho|iny|—9H-purine; 8—(1H-Indolyl)((R)—3-methy|—morpholinyl)morpho|iny|—9H-purine; 8—(1H-Indolyl)—2,6-bis—((S)—3-methy|—morpho|inyl)—9H-purine; 8—(1H-Indolyl)((S)methy|—morpho|inyl)morpho|iny|—9H-purine; 8—(1H-Indolyl)((S)methy|—morpho|inyl)morpho|iny|—9H-purine; 8—(1H-Indolyl)—2—(4-methoxy-piperidiny|)morpho|inyI-9H-purine; 8—(1H-Indolyl)—2,6-di-morpholinyI-9H-purine; 8—(1H-Indazolyl)—2,6-di-morpholinyI-9H-purine; 8—(1H-Indolyl)—2,6-di-morpholinyI-9H-purine; 3-(2,6-Di-morpholinyI-9H-puriny|)-pheny|amine; N-[3-(2,6-Di-morpholinyI-9H-purinyl)—pheny|]—acetamide; 8—(2—MethyI-1H-indolyl)—2,6-di-morpholinyI-9H-purine; 3-[2—((28,6R)—2,6-DimethyI-morpholinyl)—6-morpholinyI-9H-puriny|]-pheny|amine; N-[3-(2,6-Di-morpholinyI-9H-purinyl)-pheny|]—methanesulfonamide; {2-[2—((28,6R)—2,6-Dimethy|—morpholinyl)—6-morpholinyI-9H-purin-8—y|]-pheny|}- methanol; [2-(2,6-Di-morpholinyI-9H-purinyl)-pheny|]—methano|; 3-(2,6-Di-morpholinyI-9H-purin-8—y|)-pheno|; 6-Di-morpholinyI-9H-purinyl)-pheny|]—methano|; 2-(2,6-Di-morpholinyI-9H-purin-8—y|)-pheno|; 3-[2—((28,6R)—2,6-DimethyI-morpholinyl)morpho|inyI-9H-purin-8—y|]-pheno|; 3-(2,4-dimorpholin0-7H-pyrrolo[2,3-d]pyrimidiny|)phenol; 2,6-Bis—((S)—3-methy|—morpholinyl)—8—(1H-pyrro|o[2,3-b]pyridinyl)-9H-purine; {2—FIuoro—5-[6-((S)methy|—morpho|inyl)morpho|iny|—9H-purin-8—y|]-pheny|}- methanol; 2—(4,4-Dif|uoro—piperidiny|)(1H-indoIyl)((S)methy|—morpho|inyl)—9H-purine; -[2,6-Bis—((S)methy|—morpho|inyl)—9H-purin-8—y|]—1,3-dihydro-benzoimidazoI-Z-one; {5-[2,6-Bis—((S)—3-methy|—morpho|inyl)—9H-puriny|]methoxy-pheny|}-methanol; 8—(1H-Indolyl)—2-morpholinyl(8—oxaaza-bicyclo[3.2.1]octy|)—9H-purine; 2-Methoxy[6-((S)methy|—morpho|inyl)morpho|iny|—9H-purin-8—y|]-benzoic acid; {4-Ch|oro[6-((S)methy|—morpho|inyl)morpho|iny|—9H-purin-8—y|]-pheny|}- 3-(2,6-Di-morpholinyI-9H-purin-8—y|)-benzy|amine; 1-{3-[6-((S)Methy|—morpho|inyl)morpho|iny|—9H-puriny|]-pheny|}-ethano| ; 2,6-Di-morpholinyl-8—(1H-pyrrolo[3,2—b]pyridinyl)—9H-purine; 8—(1H-Indolyl)—2,6-bis—((S)—3-methy|—morpho|inyl)—9H-purine; Indolyl)—2,6-bis—((R)—3-methy|—morpholinyl)-9H-purine; 1-[8-(1H-IndoIyl)((S)—3-methyl-morpholinyl)—9H-purin-2—y|]-piperidinol; {3-[2,6-Bis—((S)—3-methy|—morpho|inyl)—9H-puriny|]methoxy-pheny|}-methanol; 8—(1H-Indolyl)((R)—3-methyl-morpholinyl)((S)methy|—morpholinyl)—9H-purine; {3-[2,6-Bis—((R)—3-methyI-morpholinyl)—9H-puriny|]f|uoro—pheny|}-methano|; Indolyl)((R)—3-methy|—morpholinyl)morpho|iny|—9H-purine; 8—(1H-Indolyl)((R)—3-methy|—morpholinyl)morpho|iny|—9H-purine; 8—(1H-Indolyl)—2,6-bis—((S)—3-methy|—morpho|inyl)—9H-purine; 8—(1H-Indolyl)((S)methy|—morpho|inyl)morpho|iny|—9H-purine; 8—(1H-Indolyl)((S)methy|—morpho|inyl)morpho|iny|—9H-purine; 8—(1H-Indolyl)—2—(4-methoxy-piperidiny|)morpho|inyI-9H-purine; 8—(1H-Indolyl)—2,6-di-morpholinyI-9H-purine; 8—(1H-Indazolyl)—2,6-di-morpholinyI-9H-purine; 8—(1H-Indolyl)—2,6-di-morpholinyI-9H-purine; 3-(2,6-Di-morpholinyI-9H-puriny|)-pheny|amine; N-[3-(2,6-Di-morpholinyI-9H-purinyl)—pheny|]—acetamide; 8—(2—MethyI-1H-indolyl)—2,6-di-morpholinyI-9H-purine; 3-[2—((28,6R)—2,6-DimethyI-morpholinyl)—6-morpholinyI-9H-puriny|]-pheny|amine; N-[3-(2,6-Di-morpholinyI-9H-purinyl)-pheny|]—methanesulfonamide; {2-[2—((28,6R)—2,6-Dimethyl-morpholinyl)—6-morpholinyl-9H-purinyl]-phenyl}- methanol; [2-(2,6-Di-morpholinyl-9H-purinyl)-phenyl]-methanol; 3-(2,6-Di-morpholinyl-9H-purinyl)-phenol; [3-(2,6-Di-morpholinyl-9H-purinyl)-phenyl]-methanol; 2—(2,6-Di-morpholinyl-9H-purinyl)-phenol; 6-(3,3-Dimethyl-morpholinyl)(1H-indolyl)morpholinyl-9H-purine; 6-(3,3-Dimethyl-morpholinyl)(1H-indolyl)morpholinyl-9H-purine; 8—(2,3-Dihydro-1H-indolyl)((S)methyl-morpholinyl)((R)—3-methyl-morpholinyl)- ine; 8—(2,3-Dihydro-1H-indolyl)—2,6-bis-((R)—3-methyl-morpholinyl)—9H-purine; 8—(1H-lndolyl)((S)methyl-morpholinyl)((R)—3-methyl-morpholinyl)—9H-purine; 8—(1H-lndolyl)((S)methyl-morpholinyl)((R)—3-methyl-morpholinyl)—9H-purine; 8—(1H-lndolyl)—2,6-bis-((R)methyl-morpholinyl)—9H-purine; lndolyl)((R)—3-methyl-morpholinyl)(8—oxaaza-bicyclo[3.2.1]oct—3-yl)—9H- purine; 8—(1H-lndolyl)((R)—3-methyl-morpholinyl)morpholinyl-9H-purine; 8—(1H-lndolyl)morpholinyl(1S,4S)oxaaza-bicyclo[2.2.1]heptyl-9H-purine; 8—(1H-lndolyl)((S)methyl-morpholinyl)morpholinyl-9H-purine; 8—(1H-lndolyl)morpholinyl(1S,4S)oxaaza-bicyclo[2.2.1]heptyl-9H-purine; 8—(1H-lndolyl)((S)methyl-morpholinyl)(8—oxaaza-bicyclo[3.2.1]octyl)-9H- purine; 8—(1H-lndolyl)((S)methyl-morpholinyl)(8—oxaaza-bicyclo[3.2.1]octyl)-9H- purine; 8—(1H-lndolyl)—2—morpholinyl(3-oxa-8—aza-bicyclo[3.2.1]octyl)-9H-purine; 8—(1H-lndolyl)—2—morpholinyl(3-oxa-8—aza-bicyclo[3.2.1]octyl)-9H-purine; 6-(1H-lndolyl)((R)—3-methyl-morpholinyl)((S)methyl-morpholinyl)—7H- yrrolo[2,3-d]pyrimidine; 6-(1H-lndolyl)-2,4-di-morpholin- 4-yl-7H-pyrrolo[2,3-d]pyrimidine; and pharmaceutically acceptable salts thereof.

On account of one or more than one asymmetrical carbon atom, which may be present in a compound of the formula (I), a corresponding compound of the a (I) may exist in pure optically active form or in the form of a mixture of optical isomers, e. g. in the form of a race- mic mixture. All of such pure optical isomers and all of their mixtures, ing the c mixtures, are part of the present invention.

As used herein, the term rs” refers to different compounds that have the same molecular formula but differ in arrangement and configuration of the atoms. Also as used herein, the term “an optical isomer” or “a stereoisomer” refers to any of the various stereo isomeric configurations which may exist for a given compound of the present invention and includes geometric isomers. It is understood that a substituent may be attached at a chiral center of a carbon atom. The term l" refers to molecules which have the property of non-superimposability on their mirror image partner, while the term al" refers to molecules which are superimposable on their mirror image r. Therefore, the invention includes enantiomers, diastereomers or racemates of the compound. “Enantiomers” are a pair of stereoisomers that are non- mposable mirror images of each other. A 1:1 mixture of a pair of enantiomers is a "racemic” mixture. The term is used to designate a racemic mixture where riate. ereoisomers” are stereoisomers that have at least two asymmetric atoms, but which are not mirror-images of each other. The absolute chemistry is specified according to the Cahn- lngold- Prelog R-S system. When a compound is a pure enantiomer the stereochemistry at each chiral carbon may be specified by either R or 8. Resolved compounds whose absolute configuration is unknown can be designated (+) or (-) depending on the direction (dextro- or levorotatory) which they rotate plane polarized light at the wavelength of the sodium D line. Certain compounds bed herein contain one or more asymmetric centers or axes and may thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that may be defined, in terms of absolute stereochemistry, as (R)— or (S)—.

Depending on the choice of the starting materials and procedures, the compounds can be present in the form of one of the possible isomers or as mixtures thereof, for example as pure optical s, or as isomer mixtures, such as racemates and diastereoisomer es, depending on the number of asymmetric carbon atoms. The present invention is meant to include all such possible isomers, including racemic mixtures, diasteriomeric mixtures and optically pure forms. Optically active (R)— and (S)- s may be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques. lfthe compound contains a double bond, the substituent may be E or Z configuration. If the compound contains a tituted lkyl, the cycloalkyl substituent may have a cis- or trans-configuration.

It is also possible that the intermediates and compounds of the present invention may exist in different tautomeric forms, and all such forms are embraced within the scope of the invention. The term "tautomer" or "tautomeric form" refers to structural isomers of different energies which are interconvertible via a low energy barrier. For example, proton tautomers (also known as prototropic tautomers) include interconversions via migration of a , such as keto—enol and imine—enamine isomerizations. A specific example of a proton tautomer is the imidazole moiety where the proton may migrate between the two ring nitrogens. Valence tautomers include interconversions by reorganization of some of the bonding electrons.

Any resulting mixtures of isomers can be separated on the basis of the physicochemical differences of the constituents, into the pure or substantially pure ric or optical isomers, diastereomers, racemates, for example, by chromatography and/or fractional crystallization.

Any resulting tes of final products or intermediates can be resolved into the optical antipodes by known methods, e.g., by separation of the diastereomeric salts f, obtained with an lly active acid or base, and liberating the optically active acidic or basic compound. In particular, a basic moiety may thus be employed to resolve the compounds of the present invention into their optical des, e.g., by fractional crystallization of a salt formed with an lly active acid, e.g., tartaric acid, dibenzoyl tartaric acid, diacetyl tartaric acid, di-0,0’-p-toluoyl ic acid, mandelic acid, malic acid or camphor—10-sulfonic acid. Racemic products can also be resolved by chiral chromatography, e.g., high pressure liquid chromatography (HPLC) using a chiral adsorbent.

As used herein, the terms “salt” or “salts” refers to an acid addition or base addition salt of a compound of the invention. “Salts” include in particular “pharmaceutical acceptable salts”.

The term “pharmaceutically acceptable salts” refers to salts that retain the biological iveness and properties of the compounds of this invention and, which typically are not biologically or othenNise undesirable. In many cases, the nds of the present ion are capable of forming acid and/or base salts by virtue of the presence of amino and/or carboxyl groups or groups similar thereto.

In one embodiment, the invention relates to a compound of the formula (I) as defined herein, in free form. In another embodiment, the invention relates to a compound of the formula (I) as defined herein, in salt form. In another embodiment, the invention relates to a compound of the formula (I) as defined herein, in acid addition salt form. In a further embodiment, the invention relates to a compound of the formula (I) as defined herein, in pharmaceutically acceptable salt form. In yet a r embodiment, the invention relates to any one of the compounds of the Examples in free form. In yet a r embodiment, the invention relates to any one of the compounds of the Examples in salt form. In yet a further embodiment, the invention relates to any one of the compounds of the Examples in acid addition salt form. In yet a further embodiment, the invention relates to any one of the compounds of the Examples in pharmaceutically acceptable salt form.

Pharmaceutically acceptable acid addition salts can be formed with inorganic acids and c acids, e.g., acetate, aspartate, benzoate, besylate, bromide/hydrobromide, bicarbonate/carbonate, bisulfate/sulfate, camphorsulfonate, de/hydrochloride, chlortheophyllonate, citrate, ethandisulfonate, fumarate, gluceptate, gluconate, glucuronate, hippurate, hydroiodide/iodide, isethionate, lactate, lactobionate, laurylsulfate, malate, maleate, malonate, mandelate, mesylate, methylsulphate, naphthoate, ate, nicotinate, e, octadecanoate, oleate, oxalate, ate, pamoate, phosphate/hydrogen phosphate/dihydrogen ate, polygalacturonate, propionate, stearate, succinate, sulfosalicylate, tartrate, tosylate and trifluoroacetate salts.

Inorganic acids from which salts can be derived include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like.

Organic acids from which salts can be derived include, for example, acetic acid, nic acid, glycolic acid, oxalic acid, maleic acid, c acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, toluenesulfonic acid, sulfosalicylic acid, and the like. Pharmaceutically acceptable base addition salts can be formed with inorganic and organic bases.

Inorganic bases from which salts can be derived include, for example, ammonium salts and metals from s | to XII of the periodic table. In certain embodiments, the salts are derived from , potassium, ammonium, calcium, magnesium, iron, silver, zinc, and copper; particularly suitable salts include ammonium, potassium, sodium, calcium and magnesium salts. c bases from which salts can be derived include, for example, primary, secondary, and tertiary , substituted amines including naturally occurring substituted amines, cyclic amines, basic ion ge resins, and the like. n organic amines e isopropylamine, benzathine, cholinate, diethanolamine, diethylamine, lysine, meglumine, piperazine and tromethamine.

The pharmaceutically acceptable salts of the present invention can be synthesized from a basic or acidic moiety, by conventional chemical methods. Generally, such salts can be prepared by ng free acid forms of these compounds with a stoichiometric amount of the appropriate base (such as Na, Ca, Mg, or K hydroxide, carbonate, bicarbonate or the like), or by reacting free base forms of these compounds with a stoichiometric amount of the appropriate acid. Such reactions are typically d out in water or in an c solvent, or in a mixture of the two. Generally, use of non-aqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile is desirable, where practicable. Lists of additional suitable salts can be found, e.g., in gton's ceutical Sciences”, 20th ed., Mack Publishing Company, Easton, Pa., (1985); and in “Handbook of Pharmaceutical Salts: Properties, Selection, and Use” by Stahl and Wermuth (Wiley-VCH, Weinheim, Germany, 2002).

Furthermore, the compounds of the present invention, including their salts, can also be obtained in the form of their hydrates, or include other solvents used for their crystallization.

The compounds of the present invention may inherently or by design form solvates with pharmaceutically acceptable solvents (including water); therefore, it is intended that the invention embrace both solvated and unsolvated forms. The term "solvate" refers to a molecular complex of a compound of the present invention (including pharmaceutically acceptable salts f) with one or more solvent molecules. Such solvent molecules are those commonly used in the ceutical art, which are known to be innocuous to the recipient, e.g., water, ethanol, and the like. The term "hydrate" refers to the x where the solvent molecule is water.

Compounds of the invention, i.e. compounds of formula (I) that n groups e of acting as donors and/or ors for hydrogen bonds may be capable of forming co-crystals with suitable co-crystal formers. These co-crystals may be prepared from compounds of formula (I) by known co-crystal forming procedures. Such procedures e grinding, g, co-subliming, co-melting, or contacting in solution compounds of formula (I) with the co-crystal former under crystallization conditions and isolating co-crystals thereby formed.

Suitable co-crystal formers include those described in . Hence the invention further provides co-crystals comprising a compound of formula (I).

The compounds of the present invention, ing salts, hydrates and solvates thereof, may inherently or by design form polymorphs.

Any formula given herein is also intended to represent unlabeled forms as well as isotopically labeled forms of the compounds. lsotopically labeled compounds have structures depicted by the formulas given herein except that one or more atoms are ed by an atom having a selected atomic mass or mass number. es of isotopes that can be incorporated into WO 04776 compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine, and chlorine, such as 2H, 3H, 11C, 13C, 14C, 15N, 18F 31P, 32P, 358, 36Cl, 125l respectively. The invention es various isotopically labeled nds as defined herein, for example those into which radioactive isotopes, such as 3H and 14C, or those into which non-radioactive isotopes, such as 2H and 13C are present. Such isotopically labelled compounds are useful in metabolic studies (with 14C), reaction kinetic studies (with, for example 2H or 3H), detection or imaging techniques, such as positron emission tomography (PET) or single-photon emission computed tomography (SPECT) including drug or substrate tissue distribution assays, or in radioactive treatment of patients. In particular, an 18F or labeled compound may be particularly desirable for PET or SPECT studies. lsotopically- labeled compounds of formula (I) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying es and ations using an appropriate isotopically-labeled reagents in place of the non-labeled reagent previously employed.

Further, substitution with heavier isotopes, particularly deuterium (i.e., 2H or D) may afford certain therapeutic advantages resulting from greater metabolic stability, for example sed in vivo half-life or reduced dosage requirements or an improvement in therapeutic index. It is understood that deuterium in this context is ed as a substituent of a compound of the formula (I). The concentration of such a heavier isotope, specifically deuterium, may be defined by the isotopic enrichment factor. The term "isotopic enrichment factor" as used herein means the ratio between the isotopic abundance and the natural abundance of a specified e. If a substituent in a compound of this ion is denoted deuterium, such nd has an isotopic enrichment factor for each designated deuterium atom of at least 3500 (52.5% deuterium incorporation at each ated deuterium atom), at least 4000 (60% deuterium incorporation), at least 4500 (67.5% deuterium oration), at least 5000 (75% deuterium incorporation), at least 5500 (82.5% deuterium incorporation), at least 6000 (90% ium incorporation), at least 6333.3 (95% deuterium incorporation), at least 6466.7 (97% deuterium incorporation), at least 6600 (99% deuterium incorporation), or at least 6633.3 (99.5% deuterium incorporation).

Pharmaceutically acceptable solvates in accordance with the invention include those wherein the solvent of crystallization may be isotopically tuted, e.g. D20, d5-acetone, d6-DMSO. nds of the present invention may be synthesized by synthetic routes that include ses analogous to those nown in the chemical arts, particularly in light of the description ned . The starting materials are generally available from commercial sources such as Aldrich Chemicals (Milwaukee, Wis.) or are readily prepared using methods well known to those skilled in the art (e.g., prepared by methods generally described in Louis F. Fieser and Mary Fieser, Reagents for Organic Synthesis, v. 1-19, Wiley, New York (1967- 1999 ed.), or Beilsteins Handbuch der schen Chemie, 4, Aufl. ed. er-Verlag, Berlin, ing supplements (also available via the Beilstein online database)).

For illustrative purposes, the reaction schemes depicted below provide potential routes for synthesizing the compounds of the t invention as well as key intermediates. For a more detailed description of the individual reaction steps, see the Examples n below.

Those skilled in the art will appreciate that other synthetic routes may be used to synthesize the inventive compounds. Although specific ng materials and reagents are depicted in the schemes and discussed below, other starting materials and reagents can be easily substituted to provide a variety of tives and/or reaction conditions. In addition, many of the compounds prepared by the methods described below can be further modified in light of this disclosure using conventional chemistry well known to those skilled in the art.

In a further aspect, the invention relates to a process for the ation of a compound of the a (I), in free form or in pharmaceutically acceptable salt form, comprising (a) when X represents N in a compound of formula (I), the on of a compound of formula (Ila) (Ila) in which R2, R3, R4, R5, R6, R7, R8, R9, R10, R“, R12, R13, R14, R15, R16 and R17 are as defined for formula (I), with a compound of formula (III) or (IV) HO OH 0 o I1 Is (III) (IV) in which R1 is as defined for formula (I), or b) when X represents CH in a compound of formula (I), the reaction of a compound of formula (llb) (IIb) in which R2, R3, R4, R5, R6, R7, R8, R9, R10, R“, R12, R13, R14, R15, R16 and R17 are as defined for formula (I) and PG is a protecting group, with a nd of formula (III) or (IV) HO OH 0 o I1 Is (III) (IV) in which R1 is as defined for formula (I), and thereafter i) the optional reduction, oxidation or other functionalisation of the ing compound, ii) the ge of any protecting group(s) present, iii) the recovery of the so obtainable compound of the formula (I) in free form or in pharmaceutically acceptable salt form, and/or iv) the optional tion of mixtures of optically active isomers into their individual optically active isomeric forms.

The reactions can be effected according to conventional methods. For example, the reaction described in step (a) above may be carried out in the presence of a suitable metal catalyst, for example tetrakis(tripheny|phosphine)palladium, a suitable base, for example cesium fluoride, a suitable solvent, for example acetonitrile/water and at a suitable temperature, for example 50 to 150°C, more suitably 90 to 130°C.

The reaction described in step (b) above may be carried out in the presence of a suitable catalyst, for example palladium (ll) acetate, a le oxidant, for example copper (ll) e, a suitable solvent, for example acetic acid, and at a suitable temperature, for example 0 to 50°C, or more suitably room temperature.

Within the scope of this text, only a readily ble group that is not a constituent of the particular desired end product of the compounds of the present invention is designated a "protecting group", unless the context indicates otherwise. The protection of functional groups by such protecting groups, the protecting groups themselves, and their cleavage reactions are described for example in standard reference works, such as J. F. W. McOmie, "Protective Groups in Organic Chemistry", Plenum Press, London and New York 1973, in T.

W. Greene and P. G. M. Wuts, "Protective Groups in Organic Synthesis", Third edition, Wiley, New York 1999, in "The es"; Volume 3 (editors: E. Gross and J. Meienhofer), ic Press, London and New York 1981, in "Methoden der organischen Chemie" (Methods of Organic Chemistry), Houben Weyl, 4th edition, Volume 15/l, Georg Thieme Verlag, Stuttgart 1974, in H.-D. Jakubke and H. Jeschkeit, "Aminosauren, Peptide, Proteine" (Amino acids, Peptides, Proteins), Verlag Chemie, Weinheim, Deerfield Beach, and Basel 1982, and in Jochen Lehmann, "Chemie der Kohlenhydrate: Monosaccharide und Derivate" (Chemistry of Carbohydrates: Monosaccharides and Derivatives), Georg Thieme Verlag, Stuttgart 1974. A characteristic of protecting groups is that they can be d readily (i.e. without the occurrence of red secondary ons) for example by solvolysis, reduction, photolysis or atively under physiological conditions (e.g. by enzymatic cleavage).

Salts of nds of the present invention having at least one salt-forming group may be prepared in a manner known to those skilled in the art. For example, salts of compounds of the present invention having acid groups may be formed, for example, by treating the compounds with metal compounds, such as alkali metal salts of suitable organic carboxylic acids, e.g. the sodium salt of 2—ethylhexanoic acid, with c alkali metal or alkaline earth metal compounds, such as the corresponding hydroxides, ates or hydrogen carbonates, such as sodium or potassium hydroxide, carbonate or en carbonate, with corresponding calcium compounds or with ammonia or a le c amine, stoichiometric amounts or only a small excess of the salt-forming agent preferably being used. Acid addition salts of compounds of the present invention are obtained in customary manner, e.g. by treating the nds with an acid or a suitable anion exchange reagent. lnternal salts of compounds of the present invention ning acid and basic salt-forming groups, e.g. a free carboxy group and a free amino group, may be formed, e.g. by the neutralisation of salts, such as acid on salts, to the isoelectric point, e.g. with weak bases, or by treatment with ion exchangers.

Salts can be converted into the free compounds in accordance with methods known to those skilled in the art. Metal and ammonium salts can be converted, for example, by treatment with suitable acids, and acid addition salts, for example, by treatment with a suitable basic agent.

For those compounds containing an asymmetric carbon atom, the compounds exist in individual optically active isomeric forms or as mixtures thereof, e.g. as racemic or diastereomeric mixtures. The present invention es both individual lly active R and S isomers as well as mixtures, e.g. racemic or diastereomeric mixtures, thereof. In addition, the present invention embraces all geometric and positional isomers. For example, if a compound of the present invention incorporates a double bond or a fused ring, both the cis- and trans— forms, as well as mixtures, are embraced within the scope of the invention.

Diastereomeric mixtures can be ted into their dual diastereoisomers on the basis of their physical chemical ences by methods well known to those skilled in the art, such as by chromatography and/or fractional crystallization. Enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active nd (e.g., chiral auxiliary such as a chiral alcohol or Mosher's acid de), separating the diastereoisomers and converting (e.g., hydrolyzing) the individual diastereoisomers to the corresponding pure enantiomers. Also, some of the compounds of the present invention may be atropisomers (e.g., substituted biaryls) and are considered as part of this invention. Enantiomers can also be separated by use of a commercially available chiral HPLC column.

The invention further includes any variant of the present processes, in which an intermediate t obtainable at any stage thereof is used as starting material and the remaining steps are carried out, or in which the starting materials are formed in situ under the reaction ions, or in which the reaction components are used in the form of their salts or optically pure material. Compounds of the invention and intermediates can also be converted into each other according to methods generally known to those skilled in the art.

For illustrative purposes, the on schemes depicted below provide potential routes for synthesizing the nds of the present invention as well as key intermediates. For a more detailed description of the individual on steps, see the Examples section below.

Those skilled in the art will appreciate that other synthetic routes may be used to synthesize the inventive compounds. gh specific starting materials and reagents are depicted in the s and discussed below, other starting materials and reagents can be easily substituted to provide a variety of derivatives and/or reaction conditions. In addition, many of the compounds prepared by the methods described below can be further modified in light of this disclosure using conventional chemistry well known to those skilled in the art.

Scheme 1. General Procedure 1 for synthesis of purine compounds R R11R12 HN R13 Cl R17 Y N \N R15 R14 VIII H—</ I A H—</ —> H N Cl Base, Solvent Base, Solvent Heat R6 R6 R7 R7 R8 R8 R9 R9 N Br2 \N 1° 11 \N H—</ R10 11 I XR R R12 —’ R R12 N/ / Base, Solvent A N N R13 N R13 R17 Y R17 Y 16 16 IX R R lla R15 R14 R15 R14 R5 R6 4 7 R R R R8 Ho OH \ / 0 o B \B/ R2 N l1 R9 III or R N N \N R10 11 —> R1_</ l A R12 . / CoupIIng Agent H N N R13 Base, Solvent R17 Y la R16 R15 R14 Generally, the compounds of formula la can be prepared according to Scheme 1 in four steps, ng from commercially available intermediate V. As to the individual steps in the scheme shown above, step one involves preparation of the ediate VII by chlorine displacement with nucleophile such as onalized morpholino intermediate VI.

Intermediate IX can be prepared by reaction of ediate VII with intermediate VIII in presence of adequate base such as diisopropylethyl amine, solvent such as dimethyl acetamide and heat . Step three es bromination of the intermediate IX to intermediate Ila that can be done utilizing bromine in appropriate solvent such as dichloromethane.

Target compounds of structure la can be prepared by coupling of intermediate II with variety of commercially available or synthesized boronic acids or esters of ure III or IV using metal catalysts most often exemplified by commercially ble palladium complexes.

Scheme 2. General procedure for synthesis of pyrrolo pyrimidine compounds R R12 R7 HN R13 CI R17 Y R9 R16 14 VIII / \ R H I i H / I —’ n N CI Base, Solvent N N/J\CI Base, Solvent H Heat X XI PG-Protection group \ 10 / \N 10 / 11 I i}? R11R12 I AR R H R12 ” 13 R Base, Solvent N N N N/ N R13 R17 Y PG Y x" 16 Ilb R lav/IXR16 R15 R14 R15 R14 HO OH \B/ N 9 III \N R10 11 . / CoupIIng Agent N N R13 Base, Solvent Y R17 Y R“ lb R15 R14 Generally, the compounds of a lb can be ed according to Scheme 2 in five steps, starting from commercially available intermediate X. As to the individual steps in the Scheme 2, step one involves preparation of intermediate XI by chlorine displacement with nucleophile such as functionalized morpholino intermediate VI. Intermediate XII can be prepared by reacting intermediate XI with ediate VIII in the presence of solvent such as 2012/050428 dimethyl acetamide, base such as diisopropylethyl amine and heat. Step three involves protection of intermediate XII to intermediate llb using adequate protection group such as benzylchloride or SEM-Chloride in the presence of base such as sodium hydride and solvent such as tetrahydrofuran. Step four es coupling of intermediate llb with adequate boronic acid in presence of adequate solvent such as acetic acid, t such as copper acetate and commercially available palladium st. Final step to target nds exemplified by ure lb involve the removal of the protection group using acid base or te catalysts such as palladium.

Scheme 3. General Procedure for synthesis of boronic esters. i0 0 j: \ / / \ o o R1—Br 1 —_—> R—B XIII catalyst, t, base heat Boronic esters of formula IV can be prepared according to Scheme 3 in one step where R1 is as bed in formula (I). The step involves reacting substituted omide or heteroarylbromide of formula XIII with bis(pinacolato)diboron in the presence of commercially available palladium catalyst, solvent such as dioxane at temperature ranging from 80°C to 120°C.

Compounds of the formula (I), in free form or in pharmaceutically acceptable salt form, hereinafter often referred to as “agents of the invention”, exhibit valuable pharmacological properties, when tested in vitro, and may, therefore, be useful in medicaments, in therapy or for use as research chemicals, for example as tool compounds.

The agents of the invention are inhibitors of class I P|3Ks and mTOR. The inhibiting properties of a compound of the invention towards class I P|3Ks and mTOR can be evaluated in tests as described hereinafter.

Biological Assays Test 1: Pl3 Kinase Assay P|3K KinaseGlo assay: 50 nL of compound dilutions were dispensed onto black 384-well low volume Non Binding Styrene (NBS) plates (Costar Cat. No. NBS#3676). L-a- phosphatidylinositol (Pl), provided as 10 mg/ml solution in ol, was transferred into a glass tube and dried under nitrogen beam. It was then resuspended in 3% Iucoside (OG) by vortexing and stored at 4°C. The KinaseGIo Luminescent Kinase Assay (Promega, Madison/WI, USA) is a homogeneous HTS method of measuring kinase activity by quantifying the amount of ATP remaining in solution following a kinase reaction. uL of a mix of PI/OG with the PI3K subtype were added (Table 1). Kinase reactions were started by addition of 5 ul of ATP-mix containing in a final volume 10 uL 10 mM TRIS-HCI pH 7.5, 3mM MgCIz, 50 mM NaCI, 0.05% CHAPS, 1mM DTT and 1 uM ATP, and occurred at room temperature. ons were stopped with 10 ul of KinaseGIo and plates were read 10 mins later in a Synergy2 reader using an integration time of 0.1 seconds per well. 2.5 uM of a pan-cIass 1 PI3 kinase inhibitor (standard) was added to the assay plates to generate the 100% inhibition of the kinase reaction, and the 0% inhibition was given by the t e (90% DMSO in water). The rd was used as a reference compound and included in all assay plates in the form of 16 on points in duplicate.

Cloning of PI3Ks The PI3Kq construct is a fusion of p850 iSH2 domain and the respective p110 isoform. The p850 fragment and p110 isoform gene were generated by PCR from first strand cDNA generated by RT-PCR from commercial RNA from placenta, testis and brain as described below.

PI3Kq constructs and proteins BV1075: The construct for Baculovirus BV-1075 was generated by a three-part ligation sed of a p85 fragment and a p110cx nt cloned into vector pBIueBac4.5. The p85 fragment was derived from plasmid p1661-2 digested with Nhe/Spe. The p1100i fragment derived from its done was verified by sequencing and used in LR410 as a SpeI/HindIII fragment. For the generation of the virus sion vector LR410 the gateway LR reaction to transfer the insert into the Gateway adapted pBIueBac4.5 (lnvitrogen) vector was used. The cloning vector pBIueBac4.5 (lnvitrogen) was digested with Nhe/Hindlll. This resulted in the construct PED 153.8. The p85 component (iSH2) was generated by PCR using ORF 318 as a template and one fonNard primer KAC1028 (5’- gctagcatgcgagaatatgatagat-tatatgaag-aatatacc) (SEQ ID NO: 1) and two e primers, KAC1029 (5’-gcctccaccac-ctccgcctg-gtttaatgctgttcatacgtttgtc) (SEQ ID NO: 2) and KAC1039 (5’- tactagtc-cgcctccac-cacctccgcctccaccacctccgcc) (SEQ ID NO: 3). The two reverse primers overlap and incorporate a 12x Gly linker and the N-terminal sequence of the p1100i gene to the Spel site. The PCR fragment was cloned into pCR2.1 TOPO (lnvitrogen). Of the resulting cIones, p1661-2 was determined to be correct by sequencing. This plasmid was 2012/050428 digested with Nhe and Spel and the resulting fragment was gel-isolated and purified for subcloning.

The p110cx g fragment was generated by enzymatic digest of clone LR410 (see above) with Spe | and l. The Spel site is in the coding region of the p1100i gene. The resulting fragment was gel-isolated and purified for sub-cloning. The g vector, pBlueBac4.5 (lnvitrogen) was prepared by enzymatic digestion with Nhe and Hindlll. The cut vector was purified with Qiagen column and then phorylated with Calf lntestine alkaline atase (CIP) (BioLabs). After completion of the CIP reaction the cut vector was again column purified to te the final vector. A three-part ligation was performed using Roche Rapid ligase and the vendor specifications. The final plasmid was verified by sequencing.

Protein sequence of BV 1075 (SEQ ID NO: 4): RfiYDQIYfifi YTRTSQEIQ KRTAIfiAbVfi TI<IbfifiQCQ TQfiRYSKfiYI fiKbKRfiGNfiK 6; EIQRI {NYD KLKSRISEII DSRRRIfifiD. {KQAAfiYQfiI DKQMNSI<PG GGGGGGGGGG 12; GIV?CIIPVG MIVTIfiCIRfi ATLITI<ifiI bKfiAR<Y3ui QLIQDfiSSYI bVSVTQfiAfiR 18; flabbDfiTRQI CDLRIEQ9b. KVIfiPVGWRfi *KIINQfiIGb AIGMPVCEFD MVKDPEVQDF 24; QRNILVVC<E AVDLRDLVS? HSRAMYVY99 VVfiSSD*I?< HIYNK4D<GQ IIVVIWVIVS 30; 9VNDKQKYT4 KINHDCV9fiQ VIAfiAIR<<T RS .LSSTQL YQG< YILKVCGCDE 36; YFL?KYPISQ YKYIQSCI 4 GRM9NLM. A {TSIYSQID DCFTM?SYSQ RISTAT9Y V 42; G?TST<S.WV I<II CATYVVVVIQ DID<IYVRTG IYiGG? .CD NVNTQRV9CS 48; N?RWN?WINY DIYI9D49RA ARJCLSICSV <GR<GA<4fii CPIAWGNINJ 4VSG 54; K AINIW9V3 HGL?)L.V?I GVTGSV9W<fi TDCufiufibDW bSSVV<b3D SVI44HAWWS 60; VSREAGFSYS HAGISNQIAQ DNfiLRfiVD<fi QI<AISTR39 QfiKD EIWS{R{YCV 66; TIP: .?<.. LSV<WNSRDE VAQ YC4V<D W39I<34QAM fiLIDCVYDDD MVRGFAVRCL 72L'EKYITDD<IS QYIIQIVQV. {YiQYIDVII VRFLI<<AIT VQRIGiFFFW HIKSTMiW<T 78; VSQQFGIII? SYCRACG Y. {{IVRQV4A 4<IIVITDII <QfiK<DfiTQ< VQMKFLVEQM 84; RR93F DAIQ GFISPIN3A{ QIGVLRI**C RIMSSA<R?I WLVWWV9DI SfiLLbQVWfiI 90; IF<VGDDIRQ DMITLQIIRI ENIWQNQG. DIRML9YGCI SIGDCVGLIE VVRNSHTI Q 96; IQC<GGJ<GA TIHQ <G?I YDAAIDIFTQ SCAGYCVATF IJGIGDQ{VS 102; NI VKDDGQJ FHIDFGHFLD {<K<KFGY<R ITQD FLIVISKGAQ fiCTKTRfibfiR 108; FQE CY<AY4 AIRQHANIFI VIFSM .GSG 9ELQSFDDI AYIRKTLALD {TfiQfiA fiYb 114; MKQ NDAHHG GWTTKMDWIF {TI<Q{ALV? .GGAHHiHHi (SEQ ID NO: 4) Purification of P|3Kd constructs P|3Kd was purified in two chromatographic steps: immobilized metal affinity chromatography (IMAC) on a Ni sepharose resin (GE Healthcare) and gel filtration ing a Superdex 200 26/60 column (GE Healthcare). All buffers were d to 4°C and lysis was performed 40 chilled on ice. Column fractionation was med at room temperature.

Typically frozen cells from 10 L of Tn5 cell culture were ended in “Lysis Buffer” 20 mM Tris-Cl, pH 7.5, 500 mM NaCl, 5% glycerol, 5 mM imidazole, 1 mM NaF, 0.1ug/mL okadaic acid (OAA), 5 mM BME, 1 x Complete protease inhibitor cocktail — EDTA—free (20 tablets/1 L buffer, Roche Applied Sciences), benzonase (25U/mL buffer, EMD Biosciences) at a ratio of 1:6 v/v pellet to Lysis Buffer ratio, and ically lysed by douncing for 20 strokes using a tight-fitting pestle. The lysate was centrifuged at 45,000 g for 30 minutes, and the supernatant was loaded onto a pre-equilibrated IMAC column (3 mL resin/100 mL Iysate).

The column was washed with 3-5 column volumes of Lysis Buffer, ed by a second wash of 3-5 column volumes with 20 mM Tris-Cl, pH 7.5, 500 mM NaCl, 5% glycerol, 45 mM ole, 1 mM NaF, 0.1 ug/mL OAA, 5 mM BME, 1x Complete se inhibitor cocktail — EDTA—free. n was eluted with 20 mM Tris-Cl, pH 7.5, 0.5 M NaCl, 5% glycerol, 250 mM imidazole, 1 mM NaF, 0.1ug/mL OAA, 5 mM BME, 1x Complete protease inhibitor cocktail — ree. Pertinent ons were analyzed by SDS—PAGE and pooled accordingly. The protein was further purified by gel filtration on a Superdex 200 26/60 column equilibrated in 20 mM Tris-Cl, pH 7.5, 0.5 M NaCl, 5% glycerol, 1 mM NaF, 5 mM DTT, 1x Complete protease inhibitor cocktail — EDTA-free. Pertinent ons were analyzed by SDS—PAGE and pooled ingly. An equal volume of Dialysis Buffer (20 mM Tris-Cl, pH 7.5, 500 mM NaCl, 50% glycerol, 5 mM NaF, 5 mM DTT) was added to the pool and than dialyzed against Dialysis Buffer two changes (one change overnight). Protein was stored at - 20°C.

Test 2: mTOR biochemical assay |C50s for mTOR interacting nds were assessed using the FRAP1/mTOR TR-FRET tracer assay (lnvitrogen by Life Technologies). FRAP1/mTOR (PV4753) and LanthaScreen i-GST Antibody (PV5594) (total volume of 14 uL) were added to each well of a ProxiPlate—384 Plus (Perkin-Elmer) ll plate. Compounds were serially diluted in DMSO (12-point, 4X dilution factor) and 1 uL of diluted compound was then added to each well and mixed by pipetting using a Biomek FX (Beckman Coulter). 5 uL of mTOR Kinase Tracer 314 (PV6087) was added to each well, mixed and plates were incubated at room temperature for 1 hour. Final concentrations of components are: 6 nM FRAP1/3 nM LanthaScreen Eu-Anti-GST Antibody/50 nM mTOR Kinase Tracer 314/unlabeled compounds, 4.8*10A'6-20uM. Final assay buffer composition is: 50 mM HEPES (pH 7.5), 50 mM NaCl, 5 mM MgClz, 1 mM EGTA, 0.01% Pluronic F-127. Plates were measured in plate reader (Perkin Elmer, EnVision) using 340 nm excitation and emission at two wavelengths, Emission-1 665 nm and Emission-2 615 nm. The TR-FRET ratio for each well (Emission-1 2012/050428 665/Emission-2 615) was plotted against compound concentration using GraphPad Prism software and |C50s were determined using nonlinear regression with outlier elimination.

Test 3: TSC Assay The following is a description of a high content g assay, utilizing TSC1-/- mouse embryonic fibroblasts (MEFs) cells, to test compounds for the inhibition of a tutively active mTOR. The assay is based on the staining of phospho-S6 (240/244) using a commercially ble antibody and detection with a fluorescently labeled secondary antibody. This assay generates |C50 values for compounds that inhibit mTOR. Here is described an imaging protocol and image ition thm to visualize and measure changes in p86 240/244 levels.

Quantification of 986 staining using high-content imaging and analysis 1. Day 0: Cell plating. Subconfluent TSC1-/— MEFs are harvested by trypsinization, resuspended in growth medium, and counted. A cell suspension of 166666 cells/mL is prepared and 30 uL is added into the wells of a 384-well plate using an electronic multichannel pipette. This results in 5000 cells/well being plated. The plates are briefly spun down and placed at 37°C and 5% C02. 2. Day 1: Cell plates are washed into a PBS starve solution (contains glucose, sodium onate, HEPES and phenol red) using a 384-well plate washer. The wash protocol aspirates the volume down to 30 uL/well before sing 60 l of the PBS starve solution. Aspiration and dispensing steps are repeated 8 times and a final volume of 30 uL/well is left. The cell plates are placed at 37°C and 5% COzfor 2hrs.

Compound treatment. Compound dose responses are prepared in DMSO. The dose responses are then diluted 1:50 in medium. 10u| of the diluted compound is added to 30u| of cells, ng a final 1:200 dilution of the al compound and final of 0.5% DMSO. Compound-treatments are performed in triplicates. The plates are placed at 37°C and 5% C02 for 2hrs.. Cells are then fixed by adding 10 uL/well 5x concentrated Mirsky‘s fixative. This results in a total volume of 50uL per well and a concentration of 1x Mirsky‘s fixative. The cell plates are briefly spun down and incubated for 1 h at room temperature. Cells are then washed using a 384-well plate washer using a protocol which tes the volume down to 30 uL/well before dispensing 60 uL/well 1X TBS.

Aspiration and dispensing steps are repeated 8 times and then an additional aspiration step leaves a final volume of 10 uL/well. Block buffer (1X TBS + 0.1% Triton X—100 + 0.1% BSA) is then added at 25 l and the plates are incubated at room temperature for 30min.. The cell plates are then aspirated down to 10 uL/well. Primary antibody (Phospho—S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb Cell Signaling #4838) is diluted 1:150 in block buffer and then 10 uL/well is added to the cell plates. Plates are incubated ght at 4°C. 3. Day 2: Cell plates are washed using 1X TBS method detailed above and then a secondary antibody solution is added at 10 uL/well (secondary antibody solution: block buffer + Hoechst 10ug/ml + goat anti-rabbit Cy5 secondary (diluted 1:150) )( Goat anti- rabbit lgG Cy5: Chemicon ational #AP187/ Hoechst 33342: lnvitrogen #H3570).

Plates are incubated for 1hr at room temperature and then washed with 1X TBS using the protocol detailed above without the final aspiration step which s in a final volume of 90 uL TBS/well . lmaging. The bottom of the plate is cleaned with 70% ethanol and then imaged using the |nCe|| 1000 automated epifluorescence cope. 10x magnification is used and 1 area (field) is imaged per well, this lly captures a total of around 400 cells per well.

Hoechst33342 images are acquired using an excitation of 360 nm (D360_40x filter), an emission of 460 nM (HQ460_40M filter) and an exposure time of 200 ms. Cy5 images are acquired using an excitation of 620 nm (Chroma 620_60X filter), an emission of 700 nM (Chroma HQ700_75M filter) and an exposure time of 200ms. A double band pass mirror is used for all . 4. Image analysis: The |nCe|| Analysis software is used to analyze the images using the Dual Object thm. First, nuclei are detected in the t33342 image using top- hat segmentation and a minimal nuclear area of 10um2. Second, cells are defined using a collar of 0.7 pm around the nuclei. The Cy5 fluorescence ity inside the collar is measured (cell intensity) and results are ed on an “Average per cell” basis. 5. |C50 calculation: |C50s are calculated by plotting the cell intensity values on the y-axis with dose response values on the . |C50 values represent nd y against mTOR.

Test 4: Autophagy assay Autophagy is a catabolic pathway that degrades bulk cytosol in lysosomal compartments enabling amino acids and fatty acids to be recycled. One of the key regulators of autophagy is the mammalian target of rapamycin (mTOR), a conserved serine/threonine kinase which suppresses the tion of the autophagic process when nutrients, growth factors and energy are available. To quantify autophagy induction by mTOR inhibitors, we use a mCherry-GFP-LC3 reporter which is amenable to retroviral delivery into mammalian cells, stable expression and analysis by fluorescence microscopy. mCherm-GFP-LCB Reporter The amino acid sequence of the mCherry-GFP-LCB construct is shown below (SEQ ID NO: ). The y sequence is underlined, GFP sequence is in bold and LCBA ce is boxed.

MVSKGEEDNMAIlKEFMRFKVHMEGSVNGHEFElEGEGEGRPYEGTQTAK GPLPFAWDlLSPQFMYGSKAYVKHPADlPDYLKLSFPEGFKWER VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA SSERMYPEDGALKGElKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV NlKLDlTSHNEDYTlVEQYERAEGRHSTGGMDELYKPVATMVSKGEELFT GVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPT LVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTR AEVKFEGDTLVNRIELKGlDFKEDGNlLGHKLEYNYNSHNVYIMADKQKN GIKVNFKlRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK DPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASNSAVD' PSDRP FKQRRSFADRCKEVQQIRDQHPSKlPVllERYKGEKQLPVLDKTKFLVPD HVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADlYEQEKDED —GFLYMVYASQETFGF Described hereinafter is an g protocol and image recognition algorithm to visualize and measure changes in the autophagic pathway.

Quantification of autophagy using high-content imaging and analysis 1. Day 0: Cell plating. Subconfluent H4 mCherry-GFP-LCB cells are harvested by trypsinization, resuspended in growth medium, and counted (H4 cells: Human neuroglioma cell line (ATCC)). A cell suspension of 66000 cells/mL is prepared and 30 uL are added into the wells of a 384-well plate using an electronic multichannel pipette.

This results in 2000 cells/well being plated. The cell plates are briefly spun down and placed at 37°C and 5% C02.

. Day 1: Compound ent. Compound dose ses are prepared in DMSO. The dose ses are then diluted 1:50 in medium. 10u| of the diluted nd is added to 30u| of cells, yielding a final 1:200 dilution of the original compound and final of 0.5% DMSO. Compound-treatments are performed in triplicates. The 384-well plates are placed at 37°C and 5% C02. Compound treatment is performed for 16-18 h (see Note 1).

. Day 2: Cell fixation. Cells are fixed by adding 10 uL/well 5x concentrated Mirsky‘s fixative mented with 25ug/mL Hoechst33342. This results in a total volume of 50uL per well and a concentration of 1x Mirsky‘s fixative and 5 ug/mL Hoechst33342.

The 384-well plate is briefly spun down and incubated for 1 h at room temperature. Cells are then washed using a 384-well plate washer using a protocol which aspirates the volume down to 10 uL/well before dispensing 100 uL/well 1X TBS. Aspiration and dispensing steps are repeated 4 times and a final volume of 100 uL/well is left. The plate is sealed using an adhesive PCR foil. . lmaging. The bottom of the plate is cleaned with 70% l and then imaged using the lnCell 1000 automated orescence microscope. 20x magnification is used and 4 different areas (fields) are imaged per well, this typically captures a total of around 400 cells per well. Hoechst33342 images are acquired using an excitation of 360 nm (D360_40x filter), an emission of 460 nM (HQ460_40M filter) and an exposure time of 150 ms. GFP images are acquired using an excitation of 475 nm (S475_20x filter), an emission of 535 nM (HQ535_50M filter) and an exposure time of 1 s. mCherry images are ed using an excitation of 535 nm (HQ535_50x filter), an on of 620 nM (HQ620_60M filter) and an exposure time of 1 s. A ple band pass mirror is used for all images.

. Image analysis. The lnCell Analysis software is used to e the images using the Multi Target Analysis algorithm. First, nuclei are detected in the Hoechst33342 image using top-hat segmentation and a minimal nuclear area of 50um2. Cells are defined using a collar of 10 um around the nuclei. Second, puncta (organelles) are identified in the mCherry image inside the cells using multi-top-hat segmentation. Third, the mask of the mCherry puncta is transferred onto the GFP image. Fourth, the GFP scence intensity inside the mCherry puncta mask is measured (reference intensity).

. The ‘organelles‘ parameter reflects mCherry-positive puncta of the mCherry-GFP-LC3 reporter and is used to calculate ‘LC3 puncta/cell’. For this purpose, the number of organelles is calculated per cell and averaged over all the cells in a given well (average per cell basis). mCherry-positive LC3 puncta numbers (y-axis) are plotted against the compound dose response values s) and EC50 values are calculated for each WO 04776 compound. EC50 values represent compound potency in terms of autophagy activation (e.g. increase in mCherry-positive L03 puncta count).

Notes 1. Autophagy-modulation and redistribution of mCherry-GFP-LCB can be already observed after a compound ent time of 3-4 h. However, more robust effects are seen with 16-18 h treatment times.

The compounds of the Examples showed the values presented in Table 1 below when tested in the above assays.

Table 1 Test 1: Test 2: Test 3: Test 4: Pl3Ka |C50 mTOR |C50 TSC1-l- |C50 Autophagy EC50 (nM) (nM) (nM) 372 458 2520 1044 1035 NT >9100 380 2987 NT = Not Tested As used herein, the term "pharmaceutically acceptable carrier" includes any and all ts, dispersion media, gs, surfactants, antioxidants, preservatives (e.g., antibacterial agents, antifungal agents), isotonic agents, tion delaying , salts, preservatives, drugs, drug stabilizers, binders, excipients, disintegration agents, lubricants, sweetening agents, flavoring agents, dyes, and the like and combinations thereof, as would be known to those skilled in the art (see, for e, Remington's Pharmaceutical Sciences, 18th Ed.

Mack Printing Company, 1990, pp. 1289- 1329). Except insofar as any conventional carrier is incompatible with the active ingredient, its use in the therapeutic or pharmaceutical compositions is contemplated.

The term "a therapeutically effective amount" of a compound of the t invention refers to an amount of the compound of the present invention that will elicit the biological or medical response of a subject, for example, reduction or inhibition of an enzyme or a protein activity, or ameliorate symptoms, alleviate conditions, slow or delay disease progression, or prevent a disease, etc. In one non-limiting embodiment, the term “a therapeutically effective amount” refers to the amount of the compound of the present invention that, when administered to a subject, is effective to (1) at least partially alleviating, inhibiting, preventing and/or ameliorating a condition, or a er or a disease (i) mediated by class I P|3K and/or mTOR or (ii) associated with class I PI3K and/or mTOR activity, or (iii) characterized by activity (normal or abnormal) of class I PI3K and/or mTOR; or (2) reducing or inhibiting the activity of class I PI3K and/or mTOR. In another non-limiting embodiment, the term “a eutically effective amount” refers to the amount of the compound of the present invention that, when administered to a cell, or a tissue, or a llular biological material, or a medium, is ive to at least lly reduce or t the activity of class I PI3K and/or mTOR. The meaning of the term “a therapeutically effective amount” as illustrated in the above embodiments for class I P|3K and/or mTOR also applies by the same means to any other relevant proteins/peptides/enzymes, such as class II or III PI3K.

As used herein, the term “subject” refers to an animal. Typically the animal is a mammal. A subject also refers to for example, primates (e.g., , male or female), cows, sheep, goats, horses, dogs, cats, rabbits, rats, mice, fish, birds and the like. In certain embodiments, the subject is a primate. In yet other embodiments, the subject is a human.

As used herein, the term “inhibit”, "inhibition" or “inhibiting” refers to the reduction or ssion of a given condition, symptom, or disorder, or disease, or a significant decrease in the baseline activity of a biological activity or process.

As used herein, the term “treat”, “treating" or "treatment" of any e or disorder refers in one embodiment, to ameliorating the disease or disorder (i.e., slowing or arresting or reducing the development of the disease or at least one of the clinical symptoms thereof). In another embodiment ”, "treating" or "treatment" refers to alleviating or ameliorating at least one physical ter including those which may not be discernible by the patient. In yet another embodiment, “treat”, "treating" or ment" refers to modulating the disease or disorder, either ally, (e.g., ization of a discernible symptom), physiologically, (e.g., stabilization of a physical parameter), or both.

As used herein, the term “prevention” of any particular disease or er refers to the administration of a compound of the invention to a subject before any symptoms of that disease or disorder are apparent.

As used herein, a subject is “in need of” a treatment if such subject would benefit biologically, medically or in quality of life from such treatment.

As used herein, the term "a, an,” "the” and similar terms used in the context of the present invention (especially in the context of the claims) are to be construed to cover both the singular and plural unless othenNise indicated herein or y contradicted by the context.

The use of any and all examples, or exemplary language (e.g. "such as”) provided herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed.

The term "compounds of the present invention" (unless specifically identified otherwise) refer to compounds of formula (I), compounds of the Examples, pharmaceutically acceptable salts of such compounds, and/or hydrates or solvates of such compounds, as well as, all stereoisomers (including diastereoisomers and enantiomers), tautomers and ically labeled compounds (including ium).

Compounds of the present invention are useful for treating diseases, conditions and disorders modulated by the inhibition of class I P|3Ks and the mTOR enzyme; uently, the compounds of the t invention (including the compositions and processes used therein) may be used in the manufacture of a medicament for the therapeutic applications described herein. Hence, another embodiment of the present invention is a pharmaceutical composition comprising a therapeutically effective amount of a compound of the present invention, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, t or carrier. In another embodiment of the present invention there is provided a pharmaceutical composition comprising a nd of the present invention, or a pharmaceutically acceptable salt f, and a pharmaceutically acceptable excipient, diluent or r.

A typical formulation is prepared by mixing a nd of the t invention and a carrier, diluent or ent. Suitable carriers, diluents and excipients are well known to those skilled in the art and include materials such as carbohydrates, waxes, water soluble and/or swellable polymers, hydrophilic or hobic materials, gelatin, oils, ts, water, and the like. The particular carrier, t or excipient used will depend upon the means and purpose for which the compound of the present invention is being applied. Solvents are generally selected based on solvents recognized by persons d in the art as safe (GRAS) to be administered to a mammal. In general, safe solvents are non-toxic aqueous solvents such as water and other non-toxic solvents that are soluble or miscible in water.

Suitable aqueous solvents include water, ethanol, propylene glycol, polyethylene glycols (e.g., PEG400, PEG300), etc. and mixtures thereof. The formulations may also include one or more buffers, stabilizing agents, surfactants, wetting agents, lubricating agents, emulsifiers, ding agents, preservatives, antioxidants, opaquing agents, glidants, processing aids, colorants, sweeteners, perfuming agents, flavoring agents and other known additives to provide an elegant presentation of the drug (Le, a compound of the present invention or pharmaceutical composition thereof) or aid in the manufacturing of the pharmaceutical product (i.e., medicament).

The formulations may be prepared using tional dissolution and mixing procedures.

For example, the bulk drug substance (i.e., nd of the present invention or stabilized form of the compound (e.g., complex with a cyclodextrin derivative or other known complexation agent)) is dissolved in a le solvent in the presence of one or more of the excipients. The compound of the present invention is typically formulated into pharmaceutical dosage forms to provide an easily controllable dosage of the drug and to give the patient an elegant and easily handleable product.

The pharmaceutical composition (or formulation) for application may be packaged in a variety of ways depending upon the method used for administering the drug. Generally, an article for distribution includes a container having ted n the pharmaceutical formulation in an appropriate form. Suitable containers are well-known to those skilled in the art and include materials such as bottles (plastic and glass), sachets, ampoules, plastic bags, metal cylinders, and the like. The container may also include a -proof assemblage to prevent indiscreet access to the contents of the e. In addition, the container has deposited thereon a label that describes the contents of the container. The label may also include appropriate warnings.

In one embodiment, the invention relates to the treatment of cellular proliferative diseases such as tumor and/or cancerous cell growth mediated by P|3K and/or mTOR. Diseases may e those g overexpression or amplification of PI3K alpha, Rheb, somatic mutation of PIK3CA or germline mutations or somatic mutation of PTEN, TSC1, TSCZ, or mutations and ocation of p850 that serve to up-regulate the p85-p110 complex. In ular, the compounds are useful in the treatment of human or animal (e.g., murine) cancers, including, for example, sarcoma; lung; bronchus; prostate; breast (including sporadic breast cancers and sufferers of Cowden disease); pancreas; intestinal cancer; colon; rectum; colon carcinoma; ctal adenoma; thyroid; liver; intrahepatic bile duct; hepatocellular; adrenal gland; stomach; gastric; glioma; glioblastoma; endometrial; melanoma; kidney; renal pelvis; urinary bladder; e corpus; uterine cervix; vagina; ovary; multiple a; esophagus; a mia; acute myelogenous ia; chronic myelogenous leukemia; lymphocytic ia; myeloid leukemia; brain; a carcinoma of the brain; oral cavity and pharynx; larynx; small intestine; non-Hodgkin lymphoma; melanoma; villous colon adenoma; a neoplasia; a neoplasia of epithelial character; lymphomas; a y carcinoma; basal cell carcinoma; squamous cell carcinoma; actinic keratosis; tumor diseases, including solid tumors; a tumor of the neck or head; polycythemia vera; es- sential thrombocythemia; myelofibrosis with myeloid metaplasia; and Walden stroem disease.

In other embodiments, the condition or disorder (e.g. Pl3K-mediated) is ed from the group consisting of: polycythemia vera, ial thrombocythemia, myelofibrosis with myeloid metaplasia, asthma, COPD, ARDS, Loffler's syndrome, eosinophilic pneumonia, parasitic (in particular metazoan) infestation (including tropical eosinophilia), bronchopulmonary aspergillosis, polyarteritis nodosa (including Churg-Strauss syndrome), eosinophilic granuloma, eosinophil-related disorders affecting the always occasioned by drug-reaction, psoriasis, contact dermatitis, atopic itis, alopecia areata, erythema multiforme, dermatitis herpetiformis, scleroderma, vitiligo, hypersensitivity is, ria, bullous pemphigoid, lupus erythematosus, pemphisus, molysis bullosa acquisita, autoimmune haematogical ers (e.g. ytic anaemia, ic anaemia, pure red cell anaemia and idiopathic ocytopenia), systemic lupus erythematosus, polychondritis, scleroderma, Wegener granulomatosis, dermatomyositis, chronic active hepatitis, myasthenia gravis, Steven-Johnson syndrome, idiopathic sprue, autoimmune inflammatory bowel disease (e.g. ulcerative colitis and Crohn's disease), ine opthalmopathy, Grave's disease, sarcoidosis, alveolitis, c hypersensitivity pneumonitis, multiple sclerosis, primary biliary cirrhosis, uveitis (anterior and posterior), interstitial lung fibrosis, psoriatic arthritis, glomerulonephritis, cardiovascular diseases, atherosclerosis, hypertension, deep venous thrombosis, stroke, myocardial infarction, unstable angina, thromboembolism, pulmonary embolism, thrombolytic diseases, acute arterial ischemia, peripheral thrombotic occlusions, and coronary artery disease, usion es, retinopathy, such as diabetic retinopathy or hyperbaric oxygen-induced pathy, and conditions characterized by elevated intraocular pressure or secretion of ocular aqueous humor, such as glaucoma.

Additional syndromes with an established or potential molecular link to dysregulation of mTOR kinase activity are, for instance, described in “K. lnoki et al. ; Disregulation of the OR y in human disease, Nature Genetics, vol 37, 19-24”; “D.M. Sabatini; mTOR and cancer: insights into a complex relationship, Nature Reviews, vol. 6, 729-734”; and in “B.T. Hennessy et al.; Exploiting the Pl3K/Akt pathway for cancer drug discovery, Nature Reviews, vol. 4, 988-1004”, and are as follows: 0 Organ or tissue lant rejection, e.g. for the treatment of recipients of e.g. heart, lung, combined heart-lung, liver, kidney, pancreatic, skin or corneal transplants; graft-versus- host disease, such as following bone marrow transplantation; . Restenosis . Tuberous sclerosis . Lymphangioleiomyomatosis . Retinitis tosis and other retinal rative disorders . Autoimmune diseases including encephalomyelitis, n-dependent diabetes mellitus, lupus, omyositis, arthritis and rheumatic es . Steroid-resistant acute Lymphoblastic Leukaemia . Fibrotic diseases including scleroderma, pulmonary fibrosis, renal fibrosis, cystic fibrosis 0 Pulmonary hypertension 0 lmmunomodulation . Multiple sclerosis . VHL syndrome . Carney complex . Familial adenonamtous polyposis . Juvenile polyposis syndrome . ogg-Duke syndrome . al hypertrophic cardiomyopathy o Wolf-Parkinson-White syndrome . Neurodegenerative disorders such as Parkinson's Disease, Huntington’s Disease, Alzheimer's Disease and dementias caused by tau mutations, spinocerebellar ataxia type 3, motor neuron disease caused by SOD1 mutations, neuronal ceroid lipofucinoses/Batten e (pediatric neurodegeneration) . wet and dry macular degeneration . muscle wasting (atrophy, cachexia) and myopathies such as Danon's disease. . bacterial and viral infections including M. tuberculosis, group A streptococcus, HSV type |, HIV infection . ibromatosis including Neurofibromatosis type 1, and . Peutz—Jeghers syndrome, Cowden’s disease.

Compounds with an tory activity on mTORC1 have shown benefit in immunomodulation and in treating proliferative diseases such as advance renal cell carcinoma or Tubero- Sclerosis (TSC) germ line mutation associated disorders.

The tic inhibition of mTOR r kinase activity or class I Pl3 kinases activity and in particular dual class I Pl3-kinase(s) and mTOR kinase inhibition may be useful for the treatment of Pl3K/Akt/mTOR pathway dependent diseases. The efficacy of a dual Pl3 kinase / mTOR inhibitor in malignant glioma has been recently described (Cancer Cell 9, 341 — 349).

For the above uses the required dosage will of course vary depending on the mode of administration, the particular ion to be treated and the effect d. In general, satisfactory results are indicated to be obtained systemically at daily dosages of from about 0.03 to about 100.0 mg/kg per body weight, e.g. about 0.03 to about 10.0 mg/kg per body weight. An indicated daily dosage in the larger mammal, e.g. humans, is in the range from about 0.5 mg to about 3 g, e.g. about 5 mg to about 1.5 g, conveniently administered, for example, in divided doses up to four times a day or in retard form. Suitable unit dosage forms for oral administration comprise from ca. 0.1 to about 500 mg, e.g. about 1.0 to about 500 mg active ingredient.

In general, compounds of the present ion will be administered as pharmaceutical compositions by any one of the following routes: oral, systemic (e.g., transdermal, intranasal or by suppository), or parenteral (e.g., intramuscular, intravenous or subcutaneous) administration. The preferred manner of stration is oral using a convenient daily dosage regimen that can be adjusted according to the degree of affliction. Compositions can take the form of tablets, pills, capsules, semisolids, powders, sustained release formulations, solutions, suspensions, elixirs, aerosols, or any other appropriate itions. Another preferred manner for administering compounds of the present ion is inhalation. This is an effective method for delivering a therapeutic agent ly to the respiratory tract.

The compounds of the present invention may be administered in free form or in pharmaceutically acceptable salt form e.g. as indicated above. Such salts may be prepared in conventional manner and exhibit the same order of activity as the free compounds.

Consequently, the invention also provides: - a method for preventing or treating conditions, disorders or diseases mediated by the activation of the P|3K (e.g. PI3 kinase alpha) and/or mTOR enzymes e.g. such as indicated above, in a subject in need of such treatment, which method ses administering to said t an effective amount of a compound of the present invention or a ceutically acceptable salt thereof. In one ment, there is ed a method for preventing or treating cancer or a neurodegenerative disorder, in a subject in need of such treatment, which method comprises administering to said subject an effective amount of a compound of the present invention or a pharmaceutically acceptable salt thereof. In another embodiment, the neurodegenerative er is Parkinson's, Huntington’s or mer's Disease. In yet another embodiment, the neurodegenerative disorder is Huntington’s e. - a compound of the present invention, or a ceutically acceptable salt thereof, for use as a medicament, e.g. in any of the methods as indicated herein. - a compound of the present invention, or a pharmaceutically able salt thereof, for use as ceutical, e.g. in any of the methods as indicated herein, in ular for the use in one or more phosphatidylinositol 3-kinase ed diseases. In one embodiment, there is provided a compound of the present invention, or a pharmaceutically acceptable salt thereof, for use in the treatment or prevention of cancer or a neurodegenerative disorder. In another ment, the neurodegenerative disorder is Parkinson's, Huntington’s or Alzheimer's e. In yet another embodiment, the neurodegenerative disorder is Huntington’s Disease. - the use of a compound of the present invention, or a pharmaceutically acceptable salt thereof, in any of the methods as indicated herein, in particular for the treatment or prevention of one or more phosphatidylinositol 3-kinase ed diseases. In one embodiment, there is provided the use of a compound of the present ion, or a pharmaceutically acceptable salt thereof, for the treatment or prevention of cancer or a neurodegenerative disorder. In another embodiment, the neurodegenerative disorder is Parkinson's, Huntington’s or Alzheimer's Disease. In yet another embodiment, the neurodegenerative disorder is Huntington’s Disease. - the use of a compound of the present invention, or a pharmaceutically acceptable salt thereof, for the manufacture of a medicament for the ent or prevention of one or more phosphatidylinositol 3-kinase mediated diseases. In one embodiment, there is provided the use of a compound of the present invention, or a pharmaceutically acceptable salt thereof, for the manufacture of a ment for the treatment or prevention of cancer or a neurodegenerative disorder. In another embodiment, the neurodegenerative disorder is Parkinson's, Huntington’s or Alzheimer's Disease. In yet another embodiment, the neurodegenerative disorder is Huntington’s Disease.

P|3K serves as a second messenger node that integrates parallel signaling pathways, ce is emerging that the combination of a P|3K inhibitor with inhibitors of other pathways will be useful in treating cancer and proliferative diseases in humans.

Approximately 20-30% of human breast cancers overexpress Her-2/neu-ErbBZ, the target for the drug trastuzumab. Although trastuzumab has demonstrated durable responses in some ts expressing Her2/neu-ErbBZ, only a subset of these patients respond. Further studies have ted that this limited se rate can be substantially improved by the combination of trastuzumab with inhibitors of PI3K or the Pl3K/AKT pathway (Chan et al., Breast Can. Res. Treat. 91:187 (2005), Woods Ignatoski et al., Brit. J. Cancer 82:666 (2000), Nagata et al., Cancer Cell 6:117 (2004)).

A variety of human malignancies express activitating mutations or increased levels of Her1/EGFR and a number of antibody and small molecule inhibitors have been developed against this receptor tyrosine kinase ing tarceva, gefitinib and erbitux. However, while EGFR inhibitors demonstrate umor activity in certain human tumors (e.g., NSCLC), they fail to increase overall patient survival in all patients with EGFR-expressing tumors. This may be rationalized by the fact that many downstream targets of Her1/EGFR are mutated or deregulated at high frequencies in a variety of malignancies, including the Pl3K/Akt pathway.

For example, gefitinib ts the growth of an adenocarcinoma cell line in in vitro assays.

Nonetheless, sub-clones of these cell lines can be selected that are ant to gefitinib that demonstrate sed activation of the Pl3/Akt pathway. Down-regulation or inhibition of this pathway renders the resistant sub-clones sensitive to nib (Kokubo et al., Brit. J.

Cancer 92:1711 (2005)). rmore, in an in vitro model of breast cancer with a cell line that harbors a PTEN mutation and over-expresses EGFR inhibition of both the kt pathway and EGFR produced a istic effect (She et al., Cancer Cell 8:287-297(2005)).

These results indicate that the combination of gefitinib and Pl3K/Akt pathway inhibitors would be an attractive therapeutic strategy in cancer.

The combination of AEE778 (an inhibitor of Her-2/neu/ErbB2, VEGFR and EGFR) and RAD001 (an inhibitor of mTOR, a downstream target of Akt) produced greater combined efficacy that either agent alone in a glioblastoma xenograft model r et al., Mol.

Cancer. Ther. 4:101-112 (2005)). strogens, such as tamoxifen, inhibit breast cancer growth through induction of cell cycle arrest that requires the action of the cell cycle inhibitor p27Kip. Recently, it has been shown that activation of the Ras-Raf—MAP Kinase pathway alters the phosphorylation status of p27Kip such that its inhibitory activity in arresting the cell cycle is attenuated, thereby contributing to anti-estrogen resistance (Donovan, et al, J. Biol. Chem. 276:40888, (2001)).

As reported by Donovan et al., inhibition of MAPK signaling through treatment with MEK inhibitor reversed the aberrant phosphorylation status of p27 in hormone refractory breast cancer cell lines and in so doing ed e sensitivity. Similarly, phosphorylation of p27Kip by Akt also abrogates its role to arrest the cell cycle (Viglietto et al., Nat Med. 8:1145 (2002)).

WO 04776 Accordingly, in a further aspect, the compounds of formula I may be useful in the ent of hormone dependent cancers, such as breast and prostate cancers. By this use, it is aimed to e hormone resistance commonly seen in these cancers with conventional anticancer agents.

In hematological cancers, such as chronic myelogenous leukemia (CML), somal translocation is responsible for the constitutively activated BCR-Abl tyrosine kinase. The afflicted patients are responsive to ib, a small molecule tyrosine kinase inhibitor, as a result of inhibition of Abl kinase activity. However, many ts with advanced stage disease respond to imatinib initially, but then relapse later due to ance-conferring mutations in the Abl kinase domain. In vitro studies have demonstrated that BCR-Ab1 employs the Ras-Raf kinase pathway to elicit its s. In addition, inhibiting more than one kinase in the same pathway provides onal protection against resistance-conferring mutations.

Accordingly, in another aspect, the compounds of the present invention are used in combination with at least one onal agent selected from the group of kinase inhibitors, such as Gleevec®, in the treatment of hematological cancers, such as chronic myelogenous leukemia (CML). By this use, it is aimed to reverse or prevent resistance to said at least one additional agent.

Because activation of the Pl3K/Akt pathway drives cell survival, inhibition of the pathway in combination with therapies that drive apoptosis in cancer cells, ing radiotherapy and chemotherapy, will result in improved responses ial et al., CA Cancer J. Clin 55:178- 194 (2005)). As an example, combination of PI3 kinase inhibitor with carboplatin demonstrated synergistic effects in both in vitro proliferation and apoptosis assays as well as in in vivo tumor efficacy in a xenograft model of ovarian cancer (Westfall and r, Mol.

Cancer Ther. 4:1764-1771 (2005)).

In addition to cancer and proliferative diseases, there is accumulating evidence that inhibitors of Class 1A and 1B Pl3 s would be therapeutically useful in others disease areas. The inhibition of p110B, the P|3K isoform product of the P|K3CB gene, has been shown to be involved in shear-induced platelet tion (Jackson et al., Nature Medicine 11:507-514 (2005)). Thus, a P|3K inhibitor that inhibits p110B would be useful as a single agent or in combination in anti-thrombotic therapy. The isoform p1106, the product of the P|K3CD gene, is important in B cell function and entiation (Clayton et al., J. Exp. Med. 196:753-763 (2002)), T-cell dependent and independent antigen responses (Jou et al., Mol. Cell. Biol. 22:8580-8590 (2002)) and mast cell differentiation (Ali et al., Nature 431 1011 ).

Thus, it is expected that p1106-inhibitors would be useful in the treatment of B-cell driven autoimmune diseases and asthma. Finally, the inhibition of p110v, the isoform t of the PI3KCG gene, results in reduced T, but not B cell, response (Reif et al., J. Immunol. 173:2236-2240 (2004)) and its inhibition demonstrates efficacy in animal models of autoimmune diseases (Camps et al., Nature Medicine 11:936-943 (2005), Barber et al., Nature Medicine 11:933-935 (2005)).

The invention further provides pharmaceutical compositions comprising at least one compound of the present invention, together with a pharmaceutically able excepient suitable for stration to a human or animal subject, either alone or together with other ncer agents.

The invention further provides methods of treating human or animal subjects suffering from a cellular proliferative disease, such as cancer. The invention thus provides methods of treating a human or animal subject in need of such treatment, comprising administering to the subject a therapeutically effective amount of a compound of the t invention either alone or in combination with one or more other ncer agents. In particular, compositions will either be formulated together as a combination therapeutic or administered tely.

Suitable anticancer agents for use with a compound of the present invention include, but are not limited to, one or more compounds selected from the group consisting of kinase inhibitors, anti-estrogens, anti androgens, other inhibitors, cancer chemotherapeutic drugs, alkylating agents, chelating agents , biological response modifiers, cancer vaccines, agents for antisense therapy as set forth below: A. Kinase lnhibitors:_Kinase tors for use as anticancer agents in conjunction with the compound of the present invention include inhibitors of Epidermal Growth Factor Receptor (EGFR) kinases such as small molecule quinazolines, for example gefitinib (US 5457105, US 5616582, and US 5770599), ZD-6474 (WO 01/32651), erlotinib (Tarceva®, US 5,747,498 and WO 96/30347), and lapatinib (US 6,727,256 and WO 02/02552); Vascular elial Growth Factor or (VEGFR) kinase inhibitors, including SU-11248 (WO 01/60814), SU 5416 (US 5,883,113 and WO 99/61422), SU 6668 (US 5,883,113 and WO 99/61422), CHIR- 258 (US 6,605,617 and US 6,774,237), vatalanib or PTK-787 (US 6,258,812), VEGF-Trap (WO 02/57423), B43-Genistein (WO-09606116), fenretinide (retinoic acid p- hydroxyphenylamine) (US 4,323,581 ), lM-862 (WO 02/62826), bevacizumab or Avastin® (WO 94/10202), KRN-951, 3-[5-(methylsulfonylpiperadine methy|)-indolyl]-quinolone, AG- 13736 and 25, o[2,1-t][1,2,4]triazines, ZK—304709, Veglin®, 601, EG- 004, 1 (US 100), Cand5 (WO 04/09769); Erb2 tyrosine kinase inhibitors such 2012/050428 as pertuzumab (WO 01/00245), trastuzumab, and rituximab; Akt protein kinase inhibitors, such as RX—0201; Protein Kinase C (PKC) inhibitors, such as LY-317615 (WO 95/17182), and perifosine (US 2003171303); Raf/Map/MEK/Ras kinase inhibitors including sorafenib (BAY 43-9006), ARQ-350RP, LErafAON, EMS-354825 AMG-548, and others disclosed in WO 03/82272; Fibroblast Growth Factor Receptor (FGFR) kinase inhibitors; Cell Dependent Kinase (CDK) inhibitors, including CYC-202 or roscovitine (WO 42 and WO 99/02162); Platelet-Derived Growth Factor or (PDGFR) kinase inhibitors such as CHlR-258, 3G3 mAb, AG-13736, SU-11248 and SU6668; and Bcr—Abl kinase inhibitors and fusion proteins such as STl-571 or Gleevec® (imatinib).

B. Anti-Estrogens:_Estrogen-targeting agents for use in anticancer therapy in conjunction with the compound of the present ion include Selective Estrogen Receptor Modulators (SERMs) including fen, toremifene, raloxifene; ase inhibitors including Arimidex® or anastrozole; Estrogen Receptor Downregulators (ERDs) including Faslodex® or fulvestrant.

C. Anti-Androgens:_Androgen-targeting agents for use in anticancer therapy in conjunction with the compound of the present invention e flutamide, bicalutamide, finasteride, aminoglutethamide, ketoconazole, and corticosteroids.

D. Other lnhibitors:_Other inhibitors for use as anticancer agents in conjunction with the compound of the present invention include protein farnesyl transferase tors including tipifarnib or R-115777 (US 2003134846 and WO 97/21701), EMS-214662, AZD-3409, and FTl-277; topoisomerase inhibitors including merbarone and diflomotecan (EN-80915); mitotic kinesin spindle protein (KSP) inhibitors including SB-743921 and MKl-833; proteasome modulators such as bortezomib or Velcade® (US 5,780,454), XL-784; and cyclooxygenase 2 (COX-2) inhibitors including eroidal antiinflammatory drugs I (NSAle).

E. Cancer herapeutic Drugs: Particular cancer chemotherapeutic agents for use as anticancer agents in conjunction with the compound of the present invention include anastrozole (Arimidex®), bicalutamide (Casodex®), cin e xane®), busulfan an®), busulfan injection (Busulfex®), capecitabine (Xeloda®), N4- ycarbonyldeoxyfluorocytidine, carboplatin (Paraplatin®), carmustine (BiCNU®), chlorambucil (Leukeran®), cisplatin (Platinol®), cladribine (Leustatin®), hosphamide (Cytoxan® or Neosar®), cytarabine, cytosine arabinoside (Cytosar—U®), cytarabine liposome injection (DepoCyt®), dacarbazine Dome®), dactinomycin (Actinomycin D, Cosmegan), daunorubicin hydrochloride (Cerubidine®), daunorubicin citrate liposome injection (DaunoXome®), dexamethasone, docetaxel (Taxotere®), doxorubicin hydrochloride (Adriamycin®, Rubex®), ide (Vepesid®), fludarabine phosphate ra®), 5- fluorouracil (Adrucil®, Efudex®), flutamide (Eulexin®), tezacitibine, Gemcitabine orodeoxycitidine), hydroxyurea (Hydrea®), ldarubicin (ldamycin®), mide (IFEX®), WO 04776 irinotecan (Camptosar®), L-asparaginase (ELSPAR®), leucovorin calcium, melphalan an®), 6-mercaptopurine (Purinethol®), methotrexate (Folex®), mitoxantrone (Novantrone®), rg, paclitaxel (Taxol®), phoenix (Yttrium90/MX—DTPA), pentostatin, polifeprosan 20 with carmustine implant (Gliadel®), tamoxifen citrate (Nolvadex®), teniposide (Vumon®), guanine, pa, zamine one®), topotecan hloride for injection (Hycamptin®), stine (Velban®), vincristine (Oncovin®), and vinorelbine (Navelbine®).

F. Alkylating Agents :_Alkylating agents for use in conjunction with the compound of the present invention include VNP-40101M or cloretizine, oxaliplatin (US 4,169,846, WO 03/24978 and WO 03/04505), glufosfamide, mafosfamide, etopophos (US 5,041,424), prednimustine; treosulfan; busulfan; irofluven (acylfulvene); penclomedine; pyrazoloacridine (PD-115934); O6-benzylguanine; decitabine (5-azadeoxycytidine); brostallicin; cin C (MitoExtra); TLK-286 (Telcyta®); temozolomide; tedin (US 5,478,932); AP-5280 nate formulation of tin); porfiromycin; and clearazide (meclorethamine).

G. Chelating Agents:_Chelating agents for use in conjunction with the compound of the present invention include tetrathiomolybdate (WO 01/60814); RP-697; Chimeric T84.66 (cT84.66); gadofosveset (Vasovist®); xamine; and bleomycin optionally in combination with electorporation (EPT).

H. Biological Response Modifiers:_BioIogical response modifiers, such as immune modulators, for use in conjunction with the compound of the present invention include staurosprine and macrocyclic analogs thereof, including UCN-01, CEP-701 and midostaurin (see WO 02/30941, WO 97/07081, WO 89/07105, US 5,621,100, WO 93/07153, WO 01/04125, WO 02/30941, WO 93/08809, WO 94/06799, WO 00/27422, WO 96/13506 and WO 88/07045); squalamine (WO 01/79255); DA-9601 (WO 98/04541 and US 6,025,387); alemtuzumab; interferons (e.g. lFN-a, lFN-b etc.); interleukins, specifically |L-2 or aldesleukin as well as |L-1, |L-3, |L-4, |L-5, |L-6, |L-7, |L-8, |L-9, |L-10, |L-11, |L-12, and active biological variants thereof having amino acid ces greater than 70% of the native human sequence; altretamine (Hexalen®); SU 101 or |ef|unomide (WO 04/06834 and US 6,331,555); imidazoquinolines such as resiquimod and mod (US 4,689,338, 5,389,640, 5,268,376, 4,929,624, 5,266,575, 5,352,784, 5,494,916, 5,482,936, 905, 5,395,937, ,238,944, and 612); and SMIPs, including benzazoles, anthraquinones, thiosemicarbazones, and nthrins (WO 04/87153, WO 04/64759, and WO 04/60308). l. Cancer Vaccines:_Anticancer vaccines for use in conjunction with the compound of the t invention include Avicine® (Tetrahedron Lett. 26:2269-70 (1974)); oregovomab (OvaRex®); Theratope® (STn-KLH); Melanoma Vaccines; GI-4000 series (GI-4014, GI- 4015, and Gl-4016), which are directed to five mutations in the Ras protein; GlioVax-1; MelaVax; Advexin® or |NGN-201 (WO 95/12660); Sig/E7/LAMP-1, encoding HPV-16 E7; 2012/050428 MAGE-3 Vaccine or M3TK (WO 94/05304); HER-2VAX; ACTIVE, which stimulates T-cells specific for tumors; GM-CSF cancer vaccine; and ia monocytogenes-based vaccines.

J. Antisense Therapy:_Anticancer agents for use in conjunction with the compound of the t invention also e antisense compositions, such as AEG-35156 (GEM-640); AP- 12009 and AP-11014 (TGF-beta2—specific antisense oligonucleotides); AVl-4126; 57; AVl-4472; oblimersen (Genasense®); JFSZ; aprinocarsen (WO 97/29780); GTl-2040 (R2 ribonucleotide reductase mRNA nse oligo) (WO 98/05769); GTl-2501 (WO 98/05769); liposome-encapsulated c-Raf antisense oligodeoxynucleotides (LErafAON) (WO 98/43095); and Sirna-027 (RNAi-based therapeutic targeting VEGFR-1 mRNA).

The compound of the present invention may also be combined in a ceutical composition with bronchiodilatory or antihistamine drugs substances. Such bronchiodilatory drugs include anticholinergic or antimuscarinic agents, in particular ipratropium bromide, oxitropium bromide, and pium bromide, and [3 receptor agonists such as salbutamol, terbutaline, salmeterol, carmoterol, milveterol and, especially, formoterol or indacaterol. Co-therapeutic antihistamine drug substances include cetirizine hydrochloride, clemastine fumarate, promethazine, loratadine, desloratadine diphenhydramine and fexofenadine hloride.

The invention provides in a further aspect a combination comprising a compound of the present invention and one or more compounds that are useful for the treatment of a thrombolytic disease, heart disease, stroke, etc. Such compounds include aspirin, a streptokinase, a tissue plasminogen activator, a urokinase, a anticoagulant, antiplatelet drugs (e.g, PLAVIX; ogrel bisulfate), a statin (e.g., LIPITOR or Atorvastatin m), ZOCOR (Simvastatin), CRESTOR (Rosuvastatin), etc.), a Beta blocker (e.g., Atenolol), NORVASC (amlodipine besylate), and an ACE inhibitor (e.g., Iisinopril).

The invention provides in a further aspect a combination comprising a compound of the present invention and one or more compounds that are useful for the treatment of antihypertension. Such nds include ACE inhibitors, lipid lowering agents such as statins, LIPITOR (Atorvastatin calcium), calcium channel blockers such as NORVASC (amlodipine besylate).

The ion provides in a further aspect a combination comprising a compound of the present invention and one or more compounds ed from the group consisting of fibrates, beta-blockers, NEPI inhibitors, ensin-2 or antagonists and platelet aggregation inhibitors.

The invention provides in a further aspect a combination comprising a compound of the present invention and a compound suitable for the ent of matory diseases, including rheumatoid arthritis. Such compound may be selected from the group consisting of TNF-0c inhibitors such as anti-TNF-oc monoclonal antibodies (such as REMICADE, GDP-870) and D2E7 (HUMIRA) and TNF receptor immunoglobulin fusion molecules (such as ENBREL), lL-1 inhibitors, receptor antagonists or soluble |L-1Roc(e.g. KINERET or ICE inhibitors), nonsterodial anti-inflammatory agents (NSAIDS), piroxicam, diclofenac, naproxen, flurbiprofen, fenoprofen, ketoprofen fen, fenamates, mefenamic acid, indomethacin, sulindac, e, pyrazolones, phenylbutazone, aspirin, COX-2 inhibitors (such as CELEBREX (celecoxib), PREXIGE (lumiracoxib)), metalloprotease inhibitors rably MMP-13 selective inhibitors), p2x7 tors, d20cinhibitors, NEUROTIN, pregabalin, low dose methotrexate, leflunomide, hydroxyxchloroquine, d-penicillamine, auranofin or parenteral or oral gold.

The invention es in a further aspect a combination comprising a compound of the present ion and a compound suitable for the treatment of osteoarthritis. Such compound may be selected from the group consisting of standard non-steroidal anti- inflammatory agents (hereinafter NSAID's) such as piroxicam, diclofenac, propionic acids such as naproxen, flurbiprofen, fenoprofen, ketoprofen and ibuprofen, fenamates such as mefenamic acid, indomethacin, sulindac, apazone, pyrazolones such as phenylbutazone, salicylates such as aspirin, COX-2 inhibitors such as celecoxib, valdecoxib, coxib and etoricoxib, analgesics and intraarticular therapies such as corticosteroids and hyaluronic acids such as hyalgan and c.

The invention es in a further aspect a combination comprising a compound of the t invention and an antiviral agent and/or an antisepsis compound. Such antiviral agent may be selected from the group consisting of Viracept, AZT, acyclovir and famciclovir. Such antisepsis compound may be selected from the group consisting of Valant.

The invention provides in a further aspect a combination comprising a compound of the present invention and one or more agents ed from the group consisting of CNS agents such as antidepressants (sertraline), anti-Parkinsonian drugs (such as yl, L-dopa, Requip, Mirapex; MAOB inhibitors (such as selegine and rasagiline); comP inhibitors (such as Tasmar); A—2 inhibitors; dopamine reuptake inhibitors; NMDA antagonists; Nicotine agonists; Dopamine ts; and inhibitors of al nitric oxide synthase).

The invention provides in a further aspect a combination comprising a compound of the present invention and one or more anti-Alzheimer's drugs. Such anti-Alzheimer Drug may be selected from the group consisting of donepezil, tacrine, d28inhibitors, NEUROTIN, pregabalin, COX-2 inhibitors, propentofylline or metryfonate.

The invention provides in a further aspect a combination sing a compound of the present ion and anosteoporosis agents and/or an immunosuppressant agent. Such osteoporosis agents may be selected from the group consisting of EVISTA (raloxifene hydrochloride), ifene, lasofoxifene or fosomax. Such immunosuppressant agents may be selected from the group consisting of FK-506 and cin.

In another aspect of the preferred embodiments, kits that include one or more compound of the present invention and a combination partner as disclosed herein are provided.

Representative kits include a P|3K inhibitor compound (e.g., a compound of the present invention) and a package insert or other labeling including directions for treating a cellular proliferative disease by administering a P|3K inhibitory amount of the compound(s).

Combined pharmaceutical compositions comprising a compound of the present ion in free form or in pharmaceutically acceptable salt form and further comprising a combination partner r in one dosage unit form or as a kit of parts) in association with at least one pharmaceutical able carrier and/or t may be manufactured in conventional manner by mixing with a pharmaceutically able carrier and/or diluent with said active ients.

Consequently, the invention provides in further s - a combination, e.g. for use in any of the methods described herein, comprising a therapeutically effective amount of a compound of the present invention, or a pharmaceutically acceptable salt thereof, and another eutic agent, for aneous or sequential administration. - a product comprising a compound of the present invention, or a pharmaceutically able salt thereof, and another therapeutic agent. - a product comprising a compound of the present invention, or a pharmaceutically able salt thereof, and another therapeutic agent as a combined preparation for use in therapy, e.g. for use in any of the therapies described herein. In one embodiment, the therapy is the treatment or prevention of cancer or a neurodegenerative disorder. In another embodiment, the therapy is the treatment or prevention of Parkinson's, Huntington’s or Alzheimer's Disease. In yet another embodiment, the therapy is the treatment or prevention of Huntington’s e. - a ed pharmaceutical composition comprising a compound of the present invention, or a pharmaceutically acceptable salt thereof, and r therapeutic agent. - a combined pharmaceutical composition sing a compound of the present invention, or a pharmaceutically acceptable salt thereof, r therapeutic agent and optionally one or more pharmaceutically acceptable carrier material(s) and/or diluents. Such ed pharmaceutical ition may be in the form of one dosage unit form or as a kit of parts. - a method as defined above comprising co-administration, e.g. itantly or in sequence, of a therapeutically effective amount of a compound of the present invention, or a pharmaceutically able salt thereof, and r therapeutic agent, e.g. as indicated above. - a pharmaceutical combination, e.g. a kit, comprising a) a first agent which is a compound of the present invention as disclosed herein, or a pharmaceutically acceptable salt thereof, and b) another therapeutic agent, e.g. as ted above; whereby such kit may comprise instructions for its administration.

The following examples of compounds of the present invention illustrate the invention without limiting the scope thereof. Methods for preparing such compounds are described hereinafter.

EXAMPLES Abbreviations Ac/ac/Oac acetyl CH3CO- AcOEt/EA/EtOAc ethyl acetate AcOH acetic acid ACN/MeCN/CchN acetonitrile Biotage flash Chromatography brs broad singlet CDCI3 deuterated chloroform CsF cesium fluoride Cu(OAc) copper(l) acetate d doublet Da dalton DAD-UV ultra violet diode array detection/ ultra violet diode array detector DCM/CHZCIZ dichloromethane deg deg ree dt doublet triplet DIPEA di-isopropylethyl amine DMA dimethylacetamide DMF dimethylformide DMSO ylsulfoxide DMSO-da ated dimethylsulfoxide dppf 1 s(diphenylphosphino)ferrocene e.g. for example equiv equivalent EtZO ethyl ether EtOH ethanol Gilston/Prep-LC HPLC - (high performance) liquid chromatography /Preparative HPLC HPLC/LC high performance liquid chromatography/liquid chromatography hr/hrs hours i-PrOH iso-propanol KOAc potassium acetate L liter LC liquid chromatography LC-MS liquid chromatography-mass spectrometry LC-UV liquid chromatography-with Ultra Violet detection MeOH methanol CDsoD deuterated methanol m et MS mass spectrometry NMP N-Methylpyrrolidone/methylpyrrolidone NMR nuclear magnetic nce 1HNMR proton nuclear magnetic resonance )2 palladium(ll) acetate PdC|2(PPh3)2 bis(triphenylphosphine) palladium(ll) chloride PdC|2(dppf) 1,1'-Bis(diphenylphosphino)ferrocene palladium (ll) chloride PPhs triphenyl phosphine RT/rt room temperature s singlet t triplet TFA trifluoroacetic acid THF tetrahydrofuran TLC thin-layer chromatography UV ultraviolet uW/MW microwave heating source wt% weight percent ANALYTICAL METHODS NMR: proton spectra are recorded on a Bruker 400 MHz ultrashield spectrometer unless otherwise noted. al shifts are reported in ppm relative to methanol (6 3.31), dimethyl sulfoxide (6 2.50), or chloroform (6 7.26). A small amount of the dry sample (2-5 mg) is dissolved in an appropriate deuterated solvent (1 mL). The shimming is automated and the spectra is obtained with 64 or more scans.

LCIMS: The sample is dissolved in suitable solvent such as MeCN, DMSO or MeOH and is injected directly into the column using the automated sample handler. The analysis is done using with one of the following methods: Method 1: compounds are analyzed on an il ODS-3 column (C18, 50 x 4.6 mm, 3 pm) with a 2 min gradient elution (20-80% itrile/H20/5 mM ammonium e) and a flow rate of 4 .

Method 2: GENERAL LC/MS method with acid mobile phase (0.1% formic acid) and fast gradient. Electrospray mass spectra (+) and (-), DAD-UV chromatogram 0 nm, scan range 120-1500 Da. Gradient: 20-80% MeCN/HZO in 2 min (2 mL/min), 2uL injection.

Column: il ODS3 C-18, 3 cm x 33 mm x 3.0 pm, 40 deg C.

Method 3: GENERAL LC/MS method with neutral mobile phase (5 mM NH4+HCOO') and fast (20-80%) gradient. Electrospray mass spectra (+) and (-), DAD-UV chromatogram 200-400 nm, scan range 120-1500 Da. Gradient: 20-80% MeCN/HZO in 2 min (2 mL/min), 2uL injection. : lnertsil ODS3 C-18, 3 cm x 33 mm x 3.0 pm, 40 deg C.

Method 4: LC/MS method for NON-POLAR (greasy) compounds with acid mobile phase (0.1% formic acid) and fast (40-90%) gradient. Electrospray mass spectra (+) and (-), DAD- UV chromatogram 200-400 nm, scan range 120-1500 Da. Gradient: 40-90% MeCN/HZO in 2 min (2 ), 2uL injection. Column: lnertsil C8-3, 3 cm x 33 mm X 3.0 pm, 40 deg C.

Method 5: LC/MS method for NON-POLAR (greasy) nds with neutral mobile phase (5mM NHIHCOO') and fast (40-90%) nt. Electrospray mass a (+) and (-), DAD-UV chromatogram 0nm, scan range 120-1500 Da. Gradient: 40-90% MeCN/HZO in 2 min (2 mL/min), 2uL injection. Column: lnertsil C8-3, 3.0 cm x 33 mm X 3.0 pm, 40 deg C.

Method 6: LC/MS method with broad range (5-95%) gradient with acid mobile phase (0.1% Formic Acid). Electrospray mass spectra (+) and (-), DAD-UV chromatogram 200-400 nm, scan range 120-1500 Da. Gradient: 5-95% MeCN/HZO in 2 min (2 mL/min), 2uL injection.

Column: lnertsil C8-3, 3.0 cm x 33 mm x 3.0 pm, 40 deg C.

Method 7: LC/MS method with broad range (5-95%) gradient with neutral mobile phase (5 mM NH4+HCOO'). Electrospray mass spectra (+) and (-), DAD-UV chromatogram 200-400 nm, scan range 120-1500 Da. Gradient: 5-95% MeCN/HZO in 2 min (2 mL/min), 2uL injection. Column: lnertsil C8-3, 3 cm x 33 mm x 3.0 pm, 40 deg C.

Method 8: LC/MS method for POLAR nds with acid mobile phase (0.1% formic acid) and slow (0-100%) nt. Electrospray mass spectra (+) and (-), DAD-UV chromatogram 200-400 nm, scan range 120-1500 Da. Gradient: 0-100% MeCN/HZO in 2 min (2 mL/min), 2uL injection. Column: lnertsil ODS3 (C-18, 3 cm x 33 mm x 3.0 pm, 40 degree C.) Method 9: LC/MS method for POLAR compounds with neutral mobile phase (5mM NHIHCOO') and slow (0-100%) gradient. Electrospray mass a (+) and (-), DAD-UV chromatogram 200-400 nm, scan range 120-1500 Da. Gradient: 0-100% MeCN/HZO in 2 min (2 mL/min), 2uL injection. Column: lnertsil ODS-3 (C-18, 3 cm x 33 mm X 3.0 pm, 40 deg C.

Method 10: Compounds are analyzed on an lnertsil ODS-3 column (C8, 30mm x 3.0 mm, 3.0 um) with a 2 min nt elution (5-90% acetonitrile/H20/5 mM ammonium formate) and a flow rate of 2 mL/min.

Method 11: Compounds are analyzed on an il ODS-3 column (C8, 30mm x 3.0 mm, 3.0 um) with a 2 min gradient elution (5-90% acetonitrile/H20/0.1% formic acid) and a flow rate of 2 mL/min.

HPLC purification utilizes a C8 or C18 column (30 x 100mm, 5 um, brand: Sunfire or XTerra).

The sample is dissolved in suitable solvent such as MeCN, DMSO or MeOH (maximum 5 mL) and is injected directly into the column using the automated sample handler. The purification is performed with an riate gradient using two methods (unless otherwise . Method 1 consists of 0.1% TFA in 5%-95% ACN in H20. Method 2 consists of 10 mM NH4OHin 5%-95% ACN in H20. sis of c ester ediates The boronic ester intermediates used in the preparation of nds of the present invention are either commercially available or may be prepared as described in the literature, or in an analogous manner, or can be ed as described ter, or in an analogous manner.

Boronic ester 1: ,5,5-Tetramethyl-[1,3,2]dioxa borolanyl)-1H-pyrrolo[2,3- b]pyridine To a solution of 4-Bromoazaindole (0.48 g, 2.41 mmol) in dioxane (12 mL) was added bis(pinacolato)diboron followed by bis(diphenylphosphino)ferrocene (0.067 g, 0.12 mmol).

The mixture was degassed for 20 minutes using nitrogen, ed by addition of PdC|2(dppf) catalyst (0.088 g, 0.12 mmol). Suspension was degassed for additional 5 minutes. Vial was seal and placed in an oil bath. The reaction was heated to 120 0C for 16 hours. After completion, the reaction mixture was allowed to cool to ambient temperature, suspension was filtered and solvent was removed under reduced pressure. The crude was directly purified on Biotage using 0-100 % gradient of EtOAC/Heptane. Further purification was done using preparartive HPLC (gradient 5%-95% with 3% n-propanol/acetonitrile over 3% npropanol /water) to afford as a white solid (0.054 g, 9.2 %) LC/MS analysis using method 7, mass (ES+) m/z 245.4 1H NMR (CDCI3) 6 9.03 (1H, brs), 8.24 (1H, d), 7.42 (1H, d), 7.30 (1H, d), 6.86 (1H,d), 1.31 (12H,s) Boronic ester 2: [2-Methoxy(4,4,5,5-tetramethyl-[1,3,2]dioxaborolanyl)-phenyl]- metha-nol Boronic ester was sized using general procedure described in Scheme 3. The compound was isolated in 77 % yield as yellow oil. 1H NMR (CD30D): 6 7.76 (1H, s), 7.67 (1H, d), 6.90 (1H, d), 4.62 (2H, s), 3.83 (3H, s), 1.32 (12H, s) Boronic ester 3: [3-Methoxy(4,4,5,5-tetramethyl-[1,3,2]dioxaborolanyl)-phenyl]- metha-nol Boronic ester was synthesized using general procedure described in Scheme 3. The compound was isolated in 27 % yield as brown solid. 1HNMR (DMSO-ds): 6 7.24 (1 H, s), 7.01 (1H, s), 6.99 (1H, s), 5.18 (1H, m), 4.47 (2H, d), 3.75 (3H, s), 1.29 (12H, s).

Boronic ester 4: BOC(4,4,5,5-Tetramethyl-[1,3,2]dioxaborolanyl)-benzylamine To a solution of 3-bromobenzylamine (0.79 g, 4.24 mmol), di-tertbutyldicarbonate (1.82 g, 8.5 mmol, 2.0 equiv) in 10 mL of CH2C|2 was added triethylamine (1.29 g, 12.7 mmol, 3.0 equiv.) After 2 hours of stirring the reaction was stopped and the solution was washed with water.

Aqueous layer was extracted with CHZCIZ. Organic layers combined and dried over MgSO4.

Upon l of t under reduced pressure, the crude was purified using flash column chromatography (Biotage, 0-60 % gradient of EtOAc/Heptane). The d boronic ester was then synthesized using general coupling procedure described in Scheme 3. The compound was isolated in 59% yield as brown solid. 1HNMR (CDCI3): 6 7.61-7.62 (2H, m), 7.27-7.31 (1H, m), 7.24 (1H, t), 4.85 (1H,brs), 4.72 (2 H, brs), 1.37 (9H,s), 1.25 (12 H, s) Example 1 Synthesis of 3-[2-((2$,6R)-2,6-Dimethyl-morpho|inyl)morpholinyl-9H-purin yl]-phenol Cw\z \ “ 12 N O“O\\\\\ a) 2-Chloromorpholinyl-9H-purine To a on of chloro-9H-purine (1.2 g, 6.35 mmol) in N,N-dimethylformamide (5 mL) was added line (553 mg, 6.35 mmol) followed by diisopropylethyl amine (1.2 mL, 6.87 mmol), dropwise via a syringe. The solution was stirred at room temperature for 1 hour until precipitate formed. The reaction mixture was then poured into water and the precipitate was filtered. Upon drying under high vacuum, 2-chloromorpholinyl-9H-purine was ed as an off-white solid. (1.5 g, 99%) LC/MS analysis method 7, mass (ES+) m/z 240.2 1H NMR (DMSO-da): 13.21 (1H, brs), 8.15 (1H, s), 4.18 (4H, brs), 3.72 (4H, t) b) 2-((2S,6R)-2,6-Dimethyl-morpho|inyl)morpholinyl-9H-purine To the solution of 2-chloromorpholinyl-9H-purine (1.5 g, 6.26 mmol) in DMA (5 mL) was added cis-2,6 dimethylmorpholine (1.42 g, 12.52 mmol), followed by DIPEA (2.1 mL, 12.02 mmol). The mixture was stirred at 130 °C for 40 hours. Upon completion of the reaction, the solution was poured into water. The s layer was extracted with EtOAc three times.

The combined organic layers were dried over NaZSO4, filtered and the solvent was removed under reduced pressure to provide 2-((2S,6R)—2,6-dimethyl-morpholinyl)morpho|inyl- 9H-purine (1.96 g, 98%) as a yellow solid. LC/MS analysis method 7 ,mass (ES+) m/z 319.2 1HNMR (DMSO-da): 12.37 (1H, s), 7.76 (1H, s), 4.38 (2H, d), 4.12 (4H, brs), 3.69 (4H, m), 3.54 (2H, m), 2.40 (2H, m), 1.14 (6H, d). c) 8-Bromo((2$,6R)-2,6-dimethyl-morpholinyl)morpholinyl-9H-purine To a solution of 2-((2S,6R)—2,6-dimethyl-morpholinyl)morpholinyl-9H-purine (860 mg, 2.70 mmol) in CH2C|2( 5 mL )was added bromine (0.04 mL, 3.24 mmol). The mixture was stirred at ambient temperature for 3 hours. Saturated sodium thiosulfate was added. The aqueous layer was extracted with romethane two times. Organic layers were combined and dried over NazSO4 and solvent was removed under reduced re. The crude was purified by flash column chromatography (0-70% EtOAc/Heptane gradient) to furnish product as an off-white solid (362.1 mg, 34%). LC/MS analysis using method 7, mass (ES+) m/z 399.1 1HNMR ): 4.31 (2H, d), 4.09 (4H, brs), 3.74 (4H, m), 3.59 (2H, m), 2.51 (2H, m), 1.18 (6H, d). d) 3-[2-((2$,6R)-2,6-Dimethyl-morpholinyl)morpholinyl-9H-purinyl]-phenol To a round bottom flask were added, o—2-((28,6R)—2,6-dimethyl-morpholiny|) morpholinyl-9H-purine (38.2 mg, 0.096 mmol), cesium fluoride 58.4 mg, 0.39 mmol), 3- hydroxyphenylboronic acid (39.8 mg, 0.29 mmol) and tetrakis(triphenylphosphine)palladium (8.9 mg, 7.69 mmol) followed by 1 mL of acetonitrile/water solvent mixture (10/1 ratio).

Suspension was heated to 115 oC and stirred ght. Upon completion of the reaction, the suspension was cooled and solids filtered off. Solvent was removed under reduced pressure and the crude was purified directly by preparative HPLC to provide 3-[2-((2S,6R)—2,6- yl-morpholinyl)morpholinyl-9H-purinyl]—phenol (18.5 mg, 47%) as yellow solid . LC/MS analysis method 7, mass (ES+) m/z 411.2, retention time 1.40 min. 1HNMR (CDCI3): 7.34 (1H, d), 7.24 (2H, m), 6.87 (1H, d), 4.35 (2H, d), 4.30 (4H, brs), 3.80 (4H, m), 3.57(2H, m), 2.53 (2H, t), 1.16 (6H, d).

Example 2 3-(2,4-Di-morpholiny|-7H-pyrrolo[2,3-d]pyrimidinyl)-phenol EN] OH .1? D 0 H a) 2,4-Di-morpholinyl-7H-pyrrolo[2,3-d]pyrimidine In a 20 mL vial, 2,4-dichloro-7H-pyrrolo[2,3-d]pyrimidine (0.19 g, 1.011 mmol, 1.0 equiv) was dissolved in NMP (2 mL). propyl-ethylamine (0.392g, 0.514 mL, 3.03 mmol, 3.0 equiv) and morpholine (0.264 g, 0.264 mL, 3.03 mmol, 3.0 equiv.) were then added. The reaction mixture was then transferred into a 5 mL microwave vessel and heated at 200°C for 30 minutes. The reaction was then cooled down to room temperature and diluted with 35 mL of ethyl acetate. The organic phase was then washed with saturated ammonium chloride solution (2 x 30 mL). The organic layer was then dried with MgSO4, filtered and removed under d pressure to obtain a brown oil. The oil was then purified using flash chromatography using 20% to 100% nt of EtOAc/Heptane. The compound was ed as a white solid (239.1 mg, 82% yield). 1HNMR (CDCI3): 6 9.67(1H, brs), 6.74(1H, m), 6.29(1H, d), 3.82(8H,dt), 3.72 (8 H, m) b) 4-Di-morpholinyl(2-trimethylsilanyl-ethoxymethyl)-7H-pyrrolo[2,3-d]pyrimidine A on of 2,4-di-morpholinyl-7H-pyrrolo[2,3-d]pyrimidine (180 mg, 0.622 mmol, 1.0 equiv) in 25 mL of DMF was cooled to 0°C using an ice bath under nitrogen atmosphere.

Sodium hydride in oil was then added (60 wt%, 34.8 mg, 0.871 mmol, 1.4 equiv) and the reaction was stirred for 1 hr at 0°C. To the reaction mixture, 2- (chloromethoxy)ethyl)trimethylsilane (154 uL, 145 mg, 0.871 mmol, 1.4 equiv) was added and the reaction was allowed to warm up to room temperature overnight. The reaction was quenched by adding 5mL of H20 then poured into 30 mL of EtOAc. The organic phase was then washed twice with aqueous saturated ammonium chloride solution (2x 25 mL). The organic layer was then dried with MgSO4, filtered and removed under reduced pressure to obtain dark brown d oil. The oil was purified by flash chromatography using 0% to 70% EtOAc/Heptane gradient. The t was isolated as a white solid (205 mg, 79% yield). 1HNMR (CDCI3): 6 6.91 (1H, d), 6.46(1H, d), 5.55 (2H, s), 3.94 (8H, dt), 3.82(8H, brs), 3.57 (2H, t), 0.95 (2H, t), 0.00 (9H, s) c) 3-[2,4-Di-morpholinyl(2-trimethylsilanyl-ethoxymethyl)-7H-pyrrolo[2,3- d]pyrimidinyl]-phenol To a 20 mL vial with a septum were added , 4-di-morpholinyl(2-trimethylsilanyl- ethoxymethyl)-7H-pyrrolo[2,3-d]pyrimidine (40 mg, 0.095 mmol, 1 equiv), palladium (ll) acetate ( 2.15 mg, 0.0095 mmol, 0.1 equiv), copper (ll) acetate (3.5 mg, 0.019 mmol, 0.2 equiv) and 3-hydroxyphenyl boronic acid (26.3 mg, 0.191 mmol, 2 equiv) followed by acetic acid (5 mL). The on was then d under 1 atm of air for 15 hours at room ature. The reaction was cooled to 0°C and aqueous saturated sodium bicarbonate solution was added slowly to quench the acid. The reaction was then diluted with ethyl acetate (40 mL) and washed with aqueous saturated bicarbonate solution (2x 20 mL). The c layer was then removed under reduced pressure. The crude product was directly purified using reverse phase preparative HPLC system (5% to 100% HZO/MeCN gradient with 0.1% TFA in water as modifier). Upon lypholizing the fractions ning the desired product, a total of 34.6 mg of C2 and C3 arylation product was obtained. The earlier fraction contained the desired C2 arylation product (8.1 mg, 16.9% yield). 1HNMR (CDCI3): 6 7.24 (1H, brs), 7.21 (1H, brs), 6.82 (1H, d), 6.44 (1H, s), 5.50 (2H, s), 3.87 (4H, m), 3.83 (4H, m), 3.79 (11H, m), 0.98 (2H, t), 0.00 (9H, s) d) 3-(2,4-Di-morpholinyl-7H-pyrrolo[2,3-d]pyrimidinyl)-phenol To a round bottom flask were added 3-[2,4-di-morpholinyl(2-trimethylsilanyl- ethoxymethyl)-7H-pyrro|o[2,3-d]pyrimidinyl]—phenol (8.1 mg, 0.016 mmol) and cesium fluoride (72.4 mg, 0.477 mmol, 30 equiv) followed by acetone (6 mL). The reaction was heated for 6 hours at 40°C. The reaction was then quenched by adding s ted ammonium chloride solution (10 mL). Acetone was removed under reduced pressure and the aqueous phase was extracted with DCM (3x 15 mL). The combined organic phase was removed under reduced pressure and the crude product was ly purified using reverse phase preparative HPLC (5% to 70% HZO/MeCN, with n-propanol in water as modifier) to obtain the desired product as a white solid(4.1mg, 67.8 % . LC/MS analysis method 7, mass (ES+) m/z 382.4, retention time 1.05 min 1HNMR (CDCI3): 6 10.15 (1 H, brs), 7.15 (1H, t), 7.02 (1H, d), 6.67 (1H, brs), 6.65 (2H, m), 6.52 (1H, s), 3.82 ppm (8H, dt), 3.64 (8H, m) Examples 3 to 38 Examples 3 to 38 in Table 2 below can be made using ures analogous to those described in Examples 1 and 2 using the appropriate boronic acid or boronic ester intermediate.

Table 2 Example Structure and Name m LCIMS Number 1H NMR (CDCI3): Method 7: 12.66 (1H, s), 8.26 (1H, s), 8.04 (1H, s), ange— 7.62 (1H, s), 7.29 Neutral (1H, s), 5.80 (1H, m), Retention 4.58 (2H, m), 4.14- Time: 1.15 3.98 (3H, m), 3.89- 3.53 (6H, m), 3.45- Mass (ES+): 3.34 (2H, m), 1.53 435.5 (3H,d), 1.41 (3H, d) 1H NMR (CD30D): 8.12 (1H, dd), 7.90 M92? 7'. (1H, m), 7-17 (1H, 0’ BroadRange- -43 (1H, brs), 5'07 Neutral (1 H, brs), 4'73 (2H’ S)’ 4 Retention 3.99 (1H, dd), 3-81 Time' 1 45 (2H, d), 3.75-3.68 m'in' (8H, m), 3.66-3.62 Mass (ES+): {2-Fluoro[6-((S)—3-methyl-morpholin 429'3 ( $1HH’ ddh 2:83.43I ( , ) Vl)morpholinyl-9H-pUFinyl]'phenyl}' ’ m), methanol 1H NMR (CD30D): 7.54 (2H, d), 7.46 Method 7: (1H, d), 7.35 (1H, m), C8- 726 (1 H, m), 7.20 BroadRange— (1H, m), 5.51 (1H, Neutral brs), 5.10 (1H, brs), Retention 4.02 (1H, dd), 3.94 Time: 1.58 (4H, m), 3.83 (2H, m), min 3.70 (1 H, m), 3.50 Mass (ES+): 2-(4,4-Difluoro-piperidiny|)(1H-indol- (1H, m), 1.97 (4H,m), 454-3 4-yl)((S)—3-methyl-morpholinyl)—9H- 1.44 (3H, d) punne 1H NMR (CD30D): 7.68 (2H, m), 7.11 Method 7: (1H, d), 5.40 (1H, C8- brs), 5.05 (1H, brs), ange— 4.66 (1 H, m), 4.24 Neutral (1 H, m), 3.99-3.92 Retention fl (2H, m), 3.81-3.67 Time: 1.02 \\““\/° (4H, m), 3.60-3.48 min \‘l‘ (2H, m), 3.25-3.13 Mass (ES+): -[2,6-Bis-((8)methyl-morpholiny|)- (2H, m), 1.39 , 451.6 9H-purinyl]—1,3-dihydro-benzoimidazol- 125 (3H, d) 2-one 1H NMR (CDCI3): 7.88 (2H, m), 6.97 (1 H, s), M3231 .45 (1H, brs), 4.77 BroadRange_ (2H, s), 4.58 (1 H, brs), Neutral 4.21 (1H, brs), 4.08 Retention (1H, brs), 4.07-3.71 Time 1 09 (11H, m), 3.56 (2H, m'in' m), 3.31 (1H, brs), Mass (ES+)_ 1.46 (3H, d),1-33 (3H, ' {5-[2,6-Bis—((S)—3-methyl-morpholinyl)— 455 6 ' 9H-purinyl]—2-methoxy-phenyl}- d) 1H NMR (CD30D): M3231 7.53 (1H, d), 7.47 (1H, BroadRan e_ d), 7.36 (1H, d), 7.24 Neutralg (1H, d), 7.20 (1H, t), Retention .17 (2H, brs), 4.50 Time_1 16 (2H, s), 3.74 (8H, m), m'in' 3.39 (ill-HI, r21); 1.95 Mass (ES+): ’ 432.5 1H NMR (DMSO-ds): 12.91 (1H, s), 8.25 M3231 (1H, s), 8.10(1H, d), BroadRan e_ 7.20 (1 H, d), 5.34 (1 H, Neutralg brs), 5.00 (1H, brs), Retention 3.98 (1 H, m), 3.88 Time 0 99 (3H, s), 3.78 (1H, m), m'in' Mass (ES+): . 337528-336429((110HH 121)), 2—Methoxy[6-((S)methyl-morpholln ' ' ’ ’ 455.6 1-29 (3H, d) yl)—2-morpholinyl-9H-purinyl]—benzoic acid 1H NMR (CD30D): Method 7: 7.79 (1H, m), 7.51 C8- (1H, m), 7.44 (1H, BroadRange— m), 5.38 (1H, brs), l 4.65 (2H, s), 4.62 (1 H, Retention 8), 3.99 (1H, dd), 3.79 Time: 1.28 (2H, m), 3.73 (8H, m), min 3.64 (1 H, m), 3.41 Mass (ES+): {4-Chloro—3-[6-((S)methyl-morpholin (1 H, m), 1.38 (3H, d) 445.6 morpholinyl-9H-purinyl]—phenyl}- methanol Method 7: 1H NMR (CD30D): B SF? 8.16 (1H, s), 8.01 (1H, roNaeutfglge' d), 7.58 (1H, t), 7.52 (1H, m), 4.28 (4H, m), Time' 0 73 4.20 (2H, s), 3.82 (4H, min- m), 3.75 (8H, m) Mass (ES+): 396.5 3-(2,6-Di-morpholinyl-9H-purinyl)— benz lamine 1H NMR (CD30D): Method 7: 8.01 (1H, s), 7.86 (1H, C8- m), 7.42 (2H, d), 5.42 BroadRange— (1H, brs), 5.08 (1H, l brs), 4.01 (1H, dd), Retention 3.82 (2H, m), 3.73 Time: 1.13 (8H, m), 3.66 (1H, m), min OH 3.47 (1 H, m), 1.49 Mass (ES+): 1-{3-[6-((S)—3-Methyl-morpholinyl)—2- (3H,d), 1.38 (3H, d) 425.5 morpholinyl-9H-purinyl]—phenyl}- ethanol 1H NMR (DMSO-ds): Meg‘gd 7' 13.05 (1H, brs), 11.50 BroadRange- (1H, s), 9.05 (1H, 3), Neutral 8.37 (1 H, s), 7.72 (1 H, Retention d), 6.59 (1H, d), 4.20 Time' 0 94 (4H, brs), 3.75 (4H, min- m), 3.664(I4IHgn3n), 3.64 Mass (ES+): ’ 407.4 H NMR (CD30D): 8.03 (1H, s), 7.64 (2H, m), 7.33 (1H, d), 6.49 Method 7: (1H, d), 5.41 (1H, C8- brs), 5.04 (1H, brs), BroadRange— 4.67 (1 H, m), 4.26 Neutral (1H, dd), 4.01 (1H, Retention dd), 3.94 (1H, dd), Time: 1.30 3.82 (2H, s), 3.80- min 3.65 (3H, m), 358- Mass (ES+): 3.43 (2H, m), 3.20 434.5 8-(1H-Indo|y|)-2,6-bis-((S)—3-methy|— (1H, m), 1.40 (3H,d), morpholinyl)-9H-purine 1H NMR (CD30D): 7.54 (1H, d), 7.47 (1H, d), 7.35 (1H, d), Meg‘gfj 7'_ 7.25 (1H, d), 7.20 (1H, BroadRange_ t), 5.53 (1H, brs), 5.11 Neutral (1 H, brs), 4.68 (1H, Retention m), 4.28 (1H, m), 4.03 Time 1 45 (1H, m), 3.99 (1H, m), ”1111' 3.85 (2H, m), 3.73 (3H, m), 3.56 (2H, m), Maj???) 3.25 (1H, m), 1.45 ' (3H, d), 1.27 (3H, d) H NMR (CD30D): 7.54 (1H, d), 7.47 (1H, d), 7.35 (1H, d), Method 7: 7.25 (1H, d), 7.20 (1H, C8- t), 5.50 (1H, brs), 5.11 BroadRange— (1 H, brs), 4.45 (2H, l m), 4.04 (1H, m), Retention 3.85 (2H, m), 3.81 Time: 1.28 (1H, m), 3.70 (1H, m), min 3.48 (1 H, m), 3.18 Mass (ES+): 1-[8-(1H-IndoIyl)((S)—3-methy|— (2H, m), 1.89 (2H, m), 434.4 morpholiny|)-9H-purinyl]—piperidin 1.50 (2H, m), 1.45 3H, d H NMR (CD30D): 5 7.57 (1 H,s), 7.44 (1 H,s), 7.00 (1H,s), M3231_ 5.40 (1 H,brs), 5.07 (1H,brs), 4.87 (1H,m), BroadRan e_ 4.85 (2H,s), 4.26 lg (1H,dd), 4.01 (1H,dd), Retention 3.93(1H,dd), 3.87 Time 1 30 (3H,s), 3.81 (2H,d), ”1111' 3.77-3.83 , 3.58-3.40 (2H,m), {3-[2,6-BiS-((S)methyI-morpholin-4—yl)- )- 3.25-3.13 , ' 9H-puriny|]methoxy-pheny|}_ 1.39 (3H,d), 1.25 methanol 1H NMR (CD30D): 5 7.54 (1 H,d), 7.47 (1H,d), 7.35 (1H,d), _ 7.24 (1 H,d), 7.20 Meg‘gfj 7' (1H,m), 5.49 (1H,brs), BroadRange_ .13 (1 H,brs), 4.68 Neutral (1H,m), 4.26 (1 H,dd), Retention 4.04 (1H,dd), 3.95 Time 1 21 , 3.84 (2H,s), ”1111' 3.79-3.87 (3H,m), 3.59-3.47 (2H,m), 8—(1H-IndoIyl)((R)—3-methy|— Magi?”- 3.27-3.20 (1H,m), ' morpholiny|)((S)methy|—morpho|in- 1.44 (3H,d), 1.27 4-y|)-9H-purine H NMR ): 6 8.09 (1H,dd), 7.42 (1H,m), 7.22 (1H,dd), Method 7: .43 (1 H,brs), 5.08 C8- (1 H,brs), 4.67 (1 H,m), BroadRange— 4.64 (2H,s), 4.27 Neutral (1H,dd), 4.01 (1H,dd), Retention 3.94 (1H,dd), 3.81 Time: 1.30 (2H,s), .63 min (3H,m), 3.56-3.41 Mass (ES+): {3-[2,6-Bis—((R)—3-methy|—morpholiny|)- (2H,m), 33-13 443-4 9H-puriny|]f|uoro—pheny|}-methano| (1H,m), 1-39 (3H,”, 1H NMR ): 6 .25 (1H,brs), 8.38 Meg‘gfj 7' , 7.51 (2H,t), BroadRange_ 7.39 (1H,t), 7.32 Neutral (2H,m), 5.59 (1H,brs), Retention .19 (1H,brs), 4.10 Time_1 15 (1 H,dd), 3.88 (2H,s), m'in' 3.84-3.76 (8H,m), 3.71 (1H,m), 3.61 Wig?) 8—(1H-IndoIyl)((R)—3-methy|— (1 H,m), 1,52 (3H,d) ' morpholin |morpholin-4 I-9H-purine H NMR (CDCI3): 6 11.53 (1H,brs), 8.36 (1 HS), 7.93 (1H,s), M3231 7.71 (1H,m), 7.56 BroadRange_ (1H,m), 7.31 (1H,m), Neutral 6.60 (1 HS), 5.53 Retention (1H,brs), 5.23 Time_1 19 (1 , 4.10 (2H,m), m'in' 3.88 (2H,m), 3.74 (1 H,m), 3.69 (4H,m), Wig?) IndoIyl)((R)—3-methy|— 3.60 (4H,m), 1.58 ' morpholinyl)morpho|inyI-9H-purine 1H NMR (CD30D): 6 7.54 (1 H,d), 7.47 (1H,d), 7.35 , _ 7.24 (1 H,d), 7.20 M3231 (1H,t), 5.53 (1H, brs), BroadRange_ .09 (1 H,brs), 4.69 Neutral (1H,m), 4.27 (1 H,dd), Retention 4.04 (1H,dd), 3.96 Time 1 20 (1 H,dd), 3.85 (2H,s), m'in' 3.79-3.07 (3H,m), 3.59-3.49 (2H,m), Maj???) 33-13 (1H,m), 8—(1H-IndoIyl)-2,6-bis—((S)—3-methy|— linyl)-9H-purine 1.45(3H,d), 1-27 1H NMR (CDCI3): 5 .28 (1H,brs), 8.38 Meg‘gfj 7' (1 HS), 7.50 (2H,d), BroadRange_ 7.39 (1H,m), 7.29 Neutral , 5.60 (1H,brs), Retention .20 (1H,brs), 4.11 Time_1 10 (1 H,dd), 3.88 (2H,s), m'in' 3.77 (8H,s), 3.71 Wig?)_ (1 H,m), 3.60 (1H,m), 1.51 (3H,d) ' 1H NMR (CDCI3): 5 M3231_ 12.19 (1H,brs), 8.47 (1H,s), 7.90 , BroadRange_ 7.88 , 7.29 Neutral (1H,m), 6.59 (1 HS), Retention .54 (1H,brs), 5.32 Time 1 40 (1 H,brs), 4.10 m'in' (1 H,dd), 3.89 , Wig?)_ .50 (10H,m), 1.49 (3H,m) ' 1H NMR (CDCI3): 5 Meg‘gfj 7'_ 10.5 (1 H,brs), 8.38 (1 HS), 7.50 (2H,t), Broadrange 7.38 (1H,m), 7.30 Neutral (1 H,m), 4.40 (3H,m), Retention 4.30 (3H,m), 3.91 Time_1 15 (4H,m), 3.46 (1H,m), m'in' 3.41 (3H,s), 3.37 (2H,m), 1.97 (2H,m), waif?) 1.88 (2H,m) ' Method 7: 1H NMR (CD30D)6 C8- 7.65 (1H,d), 7.62 Broadrange (1H,d), 7.44 (1H,d), Neutral 7.28 (1H,t), 7.12 Retention (1 H,d), 4.25 (4H, brs), Time: 3.90 (4H,m), 3.80 1.3 min (8H,m) Mass (ES+): 406.2 8—(1H-IndoIyl)-2,6-di-morpholinyI-9H- puflne WO 04776 Method 7: 1H NMR (DMSO-d5)5 C8- 870 (1 HS), 7.70 Broadrange— (1H,d), 7.60 (1H,d), Neutral 7.40 (1H,t), 7.32 Retention (1 H,m), 4.20 (4H,brs), Time: 3.85 (4H,m), 3.65 1.2 min (8H,m) Mass (ES+): 407.3 8-(1H-lndazolyl)-2,6-di-morpholinyl- 9H- ourine Method 7: 1H NMR (DMSO-d6)5 C8- 810 (1 HS), 7.75 Broadrange— /N , 7.60 (1H,d), Neutral I \i 7.45 (1H,m), 6.95 Retention [I C N/ Time: H N/fi (1H,s), 4.19 (4H,brs), be 3.75 (4H,m), 3.65 1.32 min H (8H,m) Mass (ES+): 406.2 8-(1H-lndolyl)-2,6-di-morpholinyl-9H- ounne [:1 Method 7: 1H NMR (CD30D)5 Broadrange— .9“; himK/o 8.15 (2H,m), 7.67 Neutral (1H,t), 7.48 (1H,m), Retention 4.35 s), 3.86 Time: , 3.80 (8H,brs) 1.16 min Mass (ES+): 382.3 3-(2,6-Di-morpholinyl-9H-purinyl)— phen lamine {:1 Method 3: 1H NMR (DMSO-ds) 5 egg? 8.22(1H,s), 7.70 Neutral (1 H,d), 7.46 (1H,t), Retention 4.20 (4H,brs), 3.75 oHOfi n1. OO Time' (4H,m), 3.65 (8H,m), '. 1.22 mIn 2.10 (3H s)’ Mass (ES+): 424.1 N-[3-(2,6-Di-morpholinyl-9H-purinyl)- phen l]-acetamide Method 3: 1H NMR (DMSO-da) 5 C18- 7.60 (1 H,d), 7.32 General- (1H,d), 7.05 (1H,t), Neutral 6.90 (1 H,s), 4.25 Retention (4H,brs), 3.80 (4H,m), Time: 3.65 (8H,m), 2.45 1.36 min (3H,s) Mass (ES+): 420.3 8-(2-MethyI-1H-indoIy|)-2,6-di- moroholin l-9H- Method 3: 1H NMR (DMSO-de) 5 C18- 7.85 (1 H,brs), 7.80 \N General- / | A (1H,m), 7.45 (1 H,t), Neutral \\“\\‘ 7.17 (1 H,d), 4.42 H N N /\V Retention (2H,d), 4.19 (4H,brs), be Time: 3.75 (4H,m), 3.55 1.35 min (2H,m), 2.45 (2H,m), Mass (ES+): 1.15 (6H,d) 410.3 3-[2-((2S,6R)—2,6-Dimethyl-morpholin yl)morpholinyl-9H-purinyl]- phen lamine Method 3: 1H NMR (DMSO-d5)6 C18- 7.89 (1 H,s), 7.76 General- , 7.43 (1H,t), Neutral 7.27 (1 H,d), 4.19 Retention (4H,brs), 3.75 , Time: 3.65 (8H,m), 3.05 (s, 1.23 min 3H) Mass (ES+): N-[3-(2,6-Di-morpholinyl-9H-purin-8—yl)- 460.2 phen |]-methanesu|fonamide Method 3: 1H NMR (CDCI3) 5 C18- N 11.0 (1H, \ N brs), 7.70 General- / l (1H, m), 7.45 (3H, Neutral / \\\ m), 4.72 (2H, s), 4.41 N “\\‘\\ N N ion H Al (2H, d), 4.24 (4H, s), Time: 0 3.88 (4H, m), 3.57 H0 (2H, m), 2.52 (2H, m), Mass (ES+): 1.23 (6H, d) 425.3 {2-[2-((2S,6R)—2,6-Dimethyl-morpholin yl)—6-morpholinyl-9H-purin-8—yl]—phenyl}- methanol E 1 Method 3: 1 C18- H NMR ) 6 General- /N \ N 7.44 (1H, m), 7-19 I A Neutral (3H, m), 4.48 (2H, 3), Retention ” N N/fi 4-0 (4H, brS) , 3-53 Time' K/o (4H, m), 3-43 (8H’ S) 1.22min Ho Mass (ES+): 397-4 [2-(2,6-Di-morpholinyI-9H-purinyl)— phen |]-methano| Method 3: N 1H NMR (DMSO-de) 5 C18- 12.88(1H, brs), 9.59 General- N \ N (1 H, s), 7.46 (2H, m), l / l X 7.25 (1 H, m), 6.79 Retention Time: N N/ (1H, m), 4.18 (4H, H “fl brs), 3.73 (4H, m), n H0 K/o 3.65 (8H, m) Mass (ES+): 383.3 3-(2,6-Di-morpholinyI-9H-purinyl)— phenol E j 1H NMR (DMSO-de) 5 N Meg? 3: 12.95(1H, brs), 8.03 General- (1H, s), 7.90 (1H, d), /N \N I 7.42 (1H, m), 7.35 Rfifggifln A (1 H, m), 5.27 (1 H, t), Time ” N N/\ 4.55 (2H, d),4.19 1 15min ' Ho K/o (4H, brs), 3.74 (4H, m), 3.64 (8H, m) Maggi”: [3-(2,6-Di-morpholinyI-9H-purinyl)— phen |]-methano| E 1 Method 3: 1 C18- H NMR (CD30D) 6 General- N \N 7.77 (1H, d), 7-28 Neutral / I X (1 H, t), 6.95 (2H, ”1), Retention N N/ 4.55 (1 H, brS) N , 4-15 Time' H fi (4H, m), 3.82 (4H, m), ' OH K/O 1.44min 3'74 (8H’ S) Mass (ES+): 2-(2,6-Di-morpholinyI-9H-purinyl)— 383.4 phenol Table 3 Example ure and name H NMR LC/MS number H NMR ((30300): Method 7; 8.04(1H, s), 7.64(2H, C8'Br03drange' Neutral m)’ 7'34(1H’ d)’ Retention 6.49(1H, d), 4.34(2H, Time:1.18 min m),4.01(2H,m), Mass (ES+): 434 3.77(8H, s), 3.52(2H, s), 1.60(6H, s) 6-(3,3-DimethyI-morpholiny|)(1H-indol- 6-yl)—2-morpholinyI-9H-purine 40 H NMR (CD30D): Method 7: C8- 7.55(1H, d), 7.49(1H, Broadrange- d), H’ d), lRetention 7.20(2H, m), 4.49(2H, .12 min m) 4.03 2H, , ( m) , Mass (ES+): 3.77(8H, s), 3.53(2H, 434.5 s), 1.62(6H, s) 6-(3,3-DimethyI-morpholiny|)(1H-indol- 4-y|)morpho|inyI-9H-purine H NMR (CD30D): Method 7: C8- 7.87(1H, d), 7.53(1H, Broadranw- t), 7.43(1H, d), NeutralRetention H, brs), Time:1.30 min .05(1H, brs), Mass (ES+): 4.60(1H, m), 4.17(1H, 436.6 d), 4.02(2H, m), 3.40- 3.90(12H, 8-(2,3-Dihydro—1H-indoIy|)((S) m),1.45(3H, d), methyl-morpho|iny|)((R)methy|- 1.32(3H, d) morpholiny|)-9H-purine H NMR (CD30D): Method 7: C8- 7.88(1H, d), 7.54(1H, Broadrange- t), 7.44(1H, d), Neutral .48(1H, brs), Retention .05(1H, brs), Time:1.30 min 4.58(1H, m), 4.20(1H, Mass (ES+): m), 4.01(2H, m), 436.6 3.37-3.88(12H, m), 8—(2,3-Dihydro—1H-indoIy|)-2,6-bis-((R)—3- 1.46(3H, d), -morpholiny|)-9H-purine 1.33(3H,d) H NMR (CD3OD): Method 7:8- 7.54(1H, m), 7.47(1H, ange- m), 7.36(1H, d), l 7.24(1H, m), 7.20(1H, Retention m), 5.49(1H, brs), Time:1.12 min .12(1H, brs), Mass (ES+): 4.68(1H, m), H, 434.5 m), 4.03(1H, m), 3.95(1H, m), 3.85(2H, 8—(1H-|ndoIyl)((S)methyl-morpholin- s), 3.76(3H, m), 4-y|)((R)methyl-morpholinyl)-9H- 3.56(2H, m), 3.24(1H, punne m), 1.44(3H, d), 1.27(3H, d) H NMR (CD3OD): Method 7: C8- 8.03(1H, s), 7.64(2H, Broad range- m), H, s), NeutralRetention 6.48(1H, s), 5.38(1H, Time:1.18 min brs), H, brs), 4.67(1H, m), 4.24(1H, Mass (ES+): m), 3.97(2H, m), 434.5 3.83(2H, s), 3.73(3H, 8—(1H-|ndoIyl)((S)methyl-morpholin- m), 3.55(2H, m), 4-y|)((R)methyl-morpholinyl)-9H- 3.20(1H, m), punne 1.39(3H, d), 1.26(3H, 1H NMR ((30300): Method 7: C8- 8.03(1H, s), 7.63(2H, Broadrange- Neutral m), H, d), 6.48(1H, d), 5.41(1H, Time:1.27 min brs), H, brs), Mass (ES+): 434.4 4.67(1H, m),4.26(1H, IndoIy|)-2,6-bis-((R)—3-methy|— m), 3.99(2H, m), morpholiny|)-9H-purine 3.83(2H, s), 3.73(3H, m), 3.55(2H, m), H, m), 1.40(3H, d), 1.26(3H, H NMR ((30300): Method 7: C8- 7.54(1H, d), H, Broadrange- d), 7.35(1H, d), Neutral 7.24(1H, d), 7.20(1H, Retention m), 5.53(1H, brs), Time:1.21 min .11(1H, brs), Mass (ES+): 4.43(2H, s), 4.25(2H, 446.5 d), 4.04(1H, m), 8—(1H-IndoIyl)((R)—3-methy|—morpholin- 3.84(2H, m), 3.70(1H, 4-y|)(8-oxaaza-bicyc|o[3.2.1]oct—3-y|)- m),3.48(1H, m), 9H-purine 3.10(2H, m), 1.88(4H, m), 1.44(3H, d) H NMR (00300): Method 7: C8- 7.53(1H, d), 7.49(1H, Broadrange- d), 7.35(1H, d), NeutralRetention 7.20(2H, m),4.69(1H, Time:1.17 min m),4.31(5H, Mass (ES+): m)3.94(1H, m), 420.5 3.85(4H, m),3.75(2H, m),3.55(1H, m), 8—(1H-IndoIyl)((R)—3-methy|—morpholin- 3.24(1H, m), 1.27(3H, 4-y|)morpho|iny|—9H-purine d) H NMR (00300): Method 7: C8- 8.04(1H, s), 7.64(2H, Broadrange- m), 7.33(1H, d), Neutr Retention 6.49(1H, d), 4.75(1H, Time:1.23 min s), 3.93(2H,m), Mass (ES+): 3.74(11H, m), 418.2 H, s) 8—(1H-Indolyl)—2-morpholiny|(18,48)- 2-oxaaza-bicyclo[2.2.1]hepty|—9H- punne H NMR (00300): Method 7: C8- 8.04(1H, s), 7.63(2H, Broad range- m), 7.33(1H, d), NeutralRetention 6.48(1H, d), 4.68(1H, Time:1.22 min m), H, m), 3.94(1H,m), Mass (ES+): H,m), 420.5 3.74(2H,m), 8—(1H-IndoIyl)((S)methyl-morpholin- 3.56(1H,m), 3.23(1H, 4-y|)morpho|iny|—9H-purine m), 1.26(3H, d) H NMR (00300): Method 7: C8- 7.54(1H, d), H, ange- d), 7.36(1H, d), Neutral 7.26(1H, m), 7.20(1H, Retention t), 4.77(1H, s), Time:1.19 min 3.97(3H, m), Mass (ES+): 3.75(10H, m), 418.3 2.07(2H, s) 8—(1H-IndoIy|)morpho|iny|(18,48)- 2-oxaaza-bicyclo[2.2.1]hepty|—9H- punne H NMR (CD30D): Method 7: C8- 7.93(1H, s), 7.53(2H, Broadrange- m), 7.23(1H, d), Neutral 6.39(1H, d), 5.31(1H, brs), 4.95(1H, brs), Retention 4.32(2H, s), 4.14(2H, .37min d), 3.91(1H, m), Mass (ES+): 3.73(2H, s), 3.58(1H, 446.4 8—(1H-IndoIyl)((S)methyl-morpholin- m), 3.37(1H, m), 4-yl)(8-oxaaza-bicyclo[3.2.1]oct—3-yl)— 3.00(2H, d), 1.70(4H, 9H-purine m), 1.29(3H, d) H NMR (CD30D): Method 7: C8- 7.54(1H, d), H, Broadrange- d), 7.35(1H, d), Neutral 724(1H1d), 720(1H’ Retention t)155. 21H( b ’ rs), Time:1.22 min H, brs).

MaSS(ES+ ) : 4.43(2H.S),4-27(2H1 446.5 d), 4.03(1H, m), 8—(1H-lndolyl)((S)methyl-morpholin- 3.85(2H, s), 3_70(1H, 4-yl)(8-oxaaza-bicyclo[3.2.1]oct—3-yl)— m), 3.48(1H, m), 9H-purine 3.09(2H, d), 1.88(4H, m), 1.45(3H, d) H NMR (CD30D): Method 7: C8- 8.04(1H, S), 7.63(2H, Broadrange- m), 7.33(1H, d), Neutral 6.49(1H, d), 5.30(2H, brS). H. d), Retention 3.74(8H, m), 3.67(2H, Time:1.32 min d), 2.12(4H, m) Mass (ES+): 432 '4 8—(1H-lndolyl)—2-morpholinyl(3-oxa- 8—aza-bicyclo[3.2.1]octyl)-9H-purine H NMR (CD30D): Method 7: C8- 7.55(1H, d), 7.48(1H, ange- d), 7.36(1H, d), Neutral 7.20(2H, m), 5.37(2H, brS), H, d), Retention 3-70(2H, d), 2-15(4H1 Time:1.18 min Mass (ES+): 432.5 lndolyl)—2-morpholinyl(3-oxa- 8—aza-bicyclo[3.2.1]octyl)-9H-purine 1H NMR (DMSO-ds): Method 7: C8- 11.66(1 H, brs), Broadrange- 11.26(1H, br), l 7.44(1 H, t), 7.35(2H, m), 7.12(1 H, t), Retention /\l 6.84(1H, d), 4.74(1H, Time:1.17 min \“k/ brs), 4.55(1H, brs), Mass (ES+): 4.41(1H, t), 4.14(1H, 433.5 t), H, d), 211,211.33.:4;.:;:.F:203pw:.::¥133rsm'mWWW_ yrro|o[2,3-d]pyrimi dine m), 3.51(4H, m), 3.16 (1H, m), 1.32 (3H, t), 1.20 (3H, m) 1H NMR (DMSO-ds): Method 7: C8- 11.52(1 H, brs), Broadrange- 11.26(1H, brs), Neutral 7.42(1H, brs), 7.34(2H, m), 7.1(1H, ion t), 6-85(2H, brS), Time:1.03 min 3.85(4H, m), 3.75(4H, Mass (ES+): m) 3.66(8H, m), 405 5 6-(1H-IndoIy|)-2,4-di-morpholin- 4-yI-7H- p rro|o[2,3-d]p rimidine \Interwoven\NRPortbl\DCC\REC\6598051_1.doc-14/08/2014 Table 4.

Example Test 1: Test 2: Test 3: Test 4: Number PI3K? IC50 (nM) mTOR IC50 (nM) - IC50 Autophagy EC50 (nM) 39 2530 160 146 2317 40 6470 350 841 >8000 41 3350 30 457 2901 42 4910 40 99 4627 43 1010 40 39 160 44 5950 140 388 395 45 7080 650 989 >400 46 580 30 100 171 47 60 60 111 461 48 4300 1010 1463 6410 49 4430 370 678 >500 50 4220 1200 920 8673 51 5940 220 228 284 52 420 50 88 386 53 >9100 190 528 782 54 5790 150 309 1253 55 1440 2670 71 1000 56 2010 2390 174 3651 The reference in this specification to any prior publication (or information derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or information derived from it) or known matter forms part of the common general knowledge in the field of endeavour to which this specification relates.

Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and ising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

Claims (1)

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:

1. A compound of formula (I), or a pharmaceutically acceptable salt thereof, X represents N or CH; R1 represents wherein R18 and R22 independently represent hydrogen, halogen, hydroxy or hydroxy-C1-3alkyl-; R19 and R21 independently represent hydrogen, amino, hydroxy, carboxy, C1-3alkoxy, amino-C1-3alkyl-, kyl-C(=O)-NH-, C1-3alkyl-S(=O)m-NH- or hydroxy-C1-3alkyl-; m ents 0, 1 or 2; R20 represents hydrogen, halogen or C1-3alkoxy; or R1 is selected from the group consisting of , , , H:\rec\Interwoven\NRPortbl\DCC\REC\6598051_1.doc-14/