NZ615694B2 - Antibodies against kidney associated antigen 1 and antigen binding fragments thereof - Google Patents

Abstract

Discloses antibodies and antigen binding fragments that bind to KAAG1 and associated methods and uses in treatment, detection and diagnosis of cancers comprising KAAG1 expressing cells, comprising heavy chain CDR sequences: GYTFTDDYMS; DINPYNGDTN; DPGAMDY; and light chain CDR sequences: RSSQSLLHSNGNTYLE; TVSNRFS; FQGSHVPLT. TYLE; TVSNRFS; FQGSHVPLT.

Description

ANTIBODIES AGAINST KIDNEY ASSOCIATED ANTIGEN 1 AND ANTIGEN BINDING FRAGMENTS THEREOF FIELD OF THE INVENTION The present invention relates to specific antibodies or antigen binding fragments that specifically bind to kidney associated n 1 (KAAG1) and their use for the treatment, detection and sis of cancer. Delivery of a therapeutic agent to cells with these antibodies or antigen binding fragments is particularly contemplated.

BACKGROUND OF THE ION Among gynecologic malignancies, ovarian cancer accounts for the highest tumor— related mortality in women in the United States (Jemal et al., 2005). It is the fourth leading cause of cancer-related death in women in the US (Menon et al., 2005). The American Cancer Society ted a total of 22,220 new cases in 2005 and attributed 16,210 deaths to the e (Bonome et al., 2005). For the past 30 years, the statistics have remained largely the same - the majority of women who develop ovarian cancer will die of this disease (Chambers and Vanderhyden, 2006). The e carries a 1:70 lifetime risk and a ity rate of >60% (Chambers and Vanderhyden, 2006). The high mortality rate is due to the difficulties with the early detection of ovarian cancer when the malignancy has already spread beyond the ovary. Indeed, >80% of ts are diagnosed with advanced staged disease (stage III or IV) (Bonome et al., 2005). These patients have a poor prognosis that is reflected in <45% 5—year survival rate, although 80% to 90% will initially respond to chemotherapy (Berek et al., 2000). This sed success compared to 20% 5-year survival rate years earlier is, at least in part, due to the ability to optimally debulk tumor tissue when it is confined to the ovaries, which is a significant prognostic factor for ovarian cancer (Bristow R. E., 2000; Brown et al., 2004). In patients who are sed with early disease (stage I), the 5-yr survival ranges from >90 (Chambers and Vanderhyden, 2006).

Ovarian cancer comprises a heterogeneous group of tumors that are derived from the surface epithelium of the ovary or from surface inclusions. They are classified into serous, mucinous, endometrioid, clear cell, and r (transitional) types corresponding to the different types of lia in the organs of the female reproductive tract (Shih and Kurman, 2005). Of these, serous tumors account for ~60% of the ovarian cancer cases diagnosed. Each histologic subcategory is further divided into three groups: benign, intermediate (borderline tumor or low ancy potential , and malignant, reflecting their clinical behavior (Seidman et al., 2002). LMP represents 10% to 15% of tumors diagnosed as serous and is a conundrum as they display atypical nuclear structure and metastatic behavior, yet they are considerably less aggressive than rade serous tumors. The 5-year survival for patients with LMP tumors is 95% in contrast to a <45% survival for advanced high-grade e over the same period (Berek et al., 2000).

Presently, the diagnosis of ovarian cancer is accomplished, in part, through routine analysis of the medical history of patients and by performing physical, ultrasound and x-ray examinations, and hematological ing. Two alternative strategies have been reported for early hematological detection of serum biomarkers. One approach is analysis of serum samples by mass spectrometry to find ns or protein fragments of unknown identity that detects the presence or absence of cancer (Mor et al., 2005; Kozak et al., 2003). However, this strategy is expensive and not broadly available. atively, the presence or absence of known proteins/peptides in the serum is being detected using antibody rrays, ELISA, or other r approaches. Serum testing for a protein biomarker called CA—125 (cancer antigen- 125) has long been widely performed as a marker for n cancer. However, although ovarian cancer cells may produce an excess of these protein les, there are some other cancers, ing cancer of the fallopian tube or endometrial cancer (cancer of the lining of the uterus), 60% of people with pancreatic cancer, and %-25% of people with other malignancies with ed levels of CA-125. The CA- 125 test only returns a true positive result for about 50% of Stage l ovarian cancer patients and has a 80% chance of returning true positive results from stage II, ill, and iv ovarian cancer patients. The other 20% of ovarian cancer patients do not show any increase in CA-125 concentrations. In addition, an elevated CA—125 test may indicate other benign activity not associated with cancer, such as menstruation, pregnancy, or endometriosis. Consequently, this test has very limited clinical ation for the detection of early stage disease when it is still treatable, ting a positive predictive value (PPV) of <10%. Even with the addition of ultrasound screening to CA- 125, the PPV only improves to around 20% (Kozak et al., 2003). Thus, this test is not an effective screening test.

Despite improved knowledge of the etiology of the disease, aggressive cytoreductive surgery, and modern combination chemotherapy, there has been only little change in mortality. Poor outcomes have been attributed to (1) lack of adequate screening tests for early disease detection in combination with only subtle presentation of symptoms at this stage - diagnosis is frequently being made only after progression to later stages, at which point the peritoneal dissemination of the cancer limits effective treatment and (2) the frequent development of resistance to standard chemotherapeutic strategies limiting improvement in the 5-year survival rate of patients. The initial chemotherapy regimen for ovarian cancer includes the combination of latin (Paraplatin) and paclitaxel ). Years of clinical trials have proved this ation to be most ive after effective surgery - reduces tumor volume in about 80% of the women with newly diagnosed ovarian cancer and 40% to 50% will have complete regression - but studies continue to look for ways to improve patient response. Recent nal infusion of chemotherapeutics to target hard-to-reach cells in combination with intravenous delivery has increased the effectiveness. However, severe side s often lead to an lete course of treatment. Some other chemotherapeutic agents include doxorubicin, cisplatin, cyclophosphamide, cin, etoposide, stine, topotecan hydrochloride, ifosfamide, 5-fluorouracil and melphalan. More recently, clinical trials have demonstrated that intraperitoneal administration of cisplatin confers a survival advantage compared to systemic intravenous chemotherapy stra and McGuire, 2007). The excellent al rates for women with early stage disease receiving chemotherapy provide a strong rationale for research efforts to develop gies to improve the detection of ovarian cancer. Furthermore, the discovery of new ovarian cancer-related biomarkers will lead to the development of more effective therapeutic strategies with l side effects for the future treatment of ovarian cancer.

Notwithstanding these recent advances in the understanding and the treatment for ovarian cancer, the use of chemotherapy is invariably associated with severe adverse reactions, which limit their use. Consequently, the need for more specific strategies such as combining n tissue specificity with the selectivity of monoclonal antibodies should permit a significant ion in rget-associated side effects.

The use of monoclonal antibodies for the therapy of ovarian cancer is beginning to emerge with an increasing number of ongoing clinical trials (Oei et al., 2008; Nicodemus and berek, 2005). Most of these trials have examined the use of monoclonal antibodies conjugated to radioisotopes, such as yttrium-90, or antibodies that target tumor antigens already identified in other cancer types. An example of this is the use of bevacizumab, which targets ar endothelial growth factor (Burger, 2007). There are very few ovarian cancer specific antigens that are currently under investigation as therapeutic targets for monoclonal antibodies. Some examples include the use of a protein termed B7-H4 (Simon et al., 2006) and more recently folate receptor-alpha (Ebel et al., 2007), the latter of which has ly entered Phase ll clinical trials.

Kidney associated antigen 1 (KAAG1) was originally cloned from a cDNA library derived from a histocompatibility leukocyte antigen-B7 renal carcinoma cell line as an antigenic peptide presented to cytotoxic T lymphocytes (Van den Eynde et al., 1999; Genebank accession no. Q9UBP8, SEQ ID NOs.:28; 29). The locus containing KAAG1 was found to encode two genes transcribed on opposite DNA strands. The sense strand was found to encode a transcript that encodes a protein termed DCDC2. Expression studies by these authors found that the KAAG1 antisense transcript was tumor specific and exhibited very little expression in normal tissues s the DCDC2 sense ript was ubiquitously expressed (Van den Eynde et al., 1999). The expression of the KAAG1 transcript in cancer, and in particular ovarian cancer, renal cancer, lung , colon cancer, breast cancer and melanoma was disclosed in the published patent application No. (the entire content of which is incorporated herein by nce). Van den Eynde et al., also observed RNA expression in renal carcinomas, colorectal omas, melanomas, sarcomas, leukemias, brain tumors, thyroid tumors, mammary carcinomas, tic carcinomas, oesophageal carcinomas, bladder tumor, lung carcinomas and head and neck tumors. Recently, strong genetic evidence obtained through linkage disequilibrium studies found that the VMP/DCDCZ/KAAG1 locus was associated with dyslexia (Schumacher et al., 2006; Cope et al., 2005). One of these reports pointed to the DCDCZ marker as the culprit in dyslexic ts since the function of this n in cortical neuron migration was in accordance with symptoms of these patients who often display abnormal neuronal migration and maturation (Schumacher et al., 2006).

SUMMARY OF THE INVENTION The invention relates to specific AAG1 antibodies and antigen binding fragments and their use for the treatment, detection and diagnosis of cancer comprising tumor cells expressing KAAG1 or a KAAG1 t. Exemplary embodiments of such cancer includes, for e, ovarian cancer, skin cancer, renal cancer, colorectal cancer, a, ia, brain cancer, cancer of the thyroid, breast cancer, prostate , cancer of the oesophagus, bladder cancer, lung cancer and head and neck cancer.

The antibodies or antigen binding fragments may be ularly effective at targeting KAAG1 or KAAG1 variant expressed at the surface of the tumor cells.

In fact, the antibodies and n binding fragments of the present invention appear to have improved ability to bind to KAAG1-expressing tumor cells in comparison with, for example, the 3D3 and 3G10 antibodies disclosed in . These antibodies and antigen binding fragments are also internalized and may ore be useful to deliver therapeutic agents to tumor cells. Our results suggest that antibodies and antigen binding nts having the desired characteristics (e.g., improved binding and internalization) generally bind to a C-terminal region of KAAG1 ted by amino acids 61 to 84. However, although both the 3A4 and 3G10 antibodies bind to the same region, the 3A4 antibody appears to bind to the surface of tumor cells more ently than the 3G10 antibody. In ular, cancer cells that express the KAAG1 antigen require approximately 10-fold less 3A4 compared to 3G10 in flow cytometry experiments, an approach that measures the direct binding of the antibodies to the surface of the cells. In addition, in binding experiments using surface plasmon resonance, it was discovered that the affinity constant (KD) of 3A4 for KAAG1 is below 10 picomolar, s antibodies 3D3 and 3G10 exhibited affinity constants (KD) greater than 200 picomolar (20-fold lower affinity). Therefore, these increases in binding ability of 3A4 are expected to translate into ed therapeutic activity.

The t invention provides in one aspect thereof, an isolated or substantially purified dy or antigen binding fragment which may be capable of specific binding to a sequence which is identical to at least 10 (e.g., 10 to 20 or more) consecutive amino acids located between amino acids 61 to 84 of KAAG1 (SEQ ID NO.:29) The present invention also provides isolated antibodies or antigen binding fragments capable of competing with the dy or antigen binding fragment described herein.

In a further aspect, the invention relates to specific antibodies or antigen binding fragments having the amino acid sequences described herein. Such antibodies or antigen binding nts may be in the form of monoclonal antibodies, polyclonal antibodies, chimeric antibodies, humanized antibodies and human antibodies (isolated) as well as antigen binding fragments having the characteristics described herein. Antibodies or antigen binding fragments encompassing permutations of the light and/or heavy chains between a monoclonal, ic, humanized or human antibody are also encompassed th.

The antibodies or antigen g fragments of the present invention may thus se amino acids of a human constant region and/or framework amino acids of a human dy.

The term "antibody” refers to intact antibody, monoclonal or polyclonal antibodies.

The term “antibody” also encompasses multispecific antibodies such as bispecific antibodies. Human antibodies are usually made of two light chains and two heavy chains each comprising variable regions and constant regions. The light chain variable region comprises 3 CDRs, identified herein as CDRL1 or L1, CDRL2 or L2 and CDRL3 or L3 flanked by framework regions. The heavy chain variable region comprises 3 CDRs, identified herein as CDRH1 or H1, CDRH2 or H2 and CDRH3 or H3 d by framework regions. The CDRs of the humanized antibodies of the present invention have been identified using the Kabat and Chotia definitions (e.g., CDRH2 set forth in SEQ ID NO.:56). However. others (Abhinandan and Martin, 2008) have used modified approaches based loosely on Kabat and Chotia resulting in the delineation of shorter CDRs (e.g., CDRH2 set forth in SEQ ID NO.:6).

The term "antigen-binding fragment", as used herein, refers to one or more fragments of an antibody that retain the ability to bind to an antigen (e.g., KAAG1, secreted form of KAAG1 or ts thereof). It has been shown that the antigen-binding on of an antibody can be performed by fragments of an intact antibody. Examples of binding fragments encompassed within the term "antigen-binding fragment" of an antibody e (i) a Fab fragment, a lent fragment ting of the VL, VH, CL and CH1 domains; (ii) a F (ab')2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CH1 domains; (iv) a Fv fragment ting of the VL and VH domains of a single arm of an antibody, (v) a dAb fragment (Ward et al., (1989) Nature 341:544-546), which consists of a VH domain; and (vi) an isolated complementarity determining region (CDR), e.g., VH CDR3. Furthermore, although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they can be joined, using recombinant methods, by a tic linker that enables them to be made as a single polypeptide chain in which the VL and V... regions pair to form monovalent molecules (known as single chain Fv (scFv); see e.g., Bird et al. (1988) Science 3-426; and Huston et at. (1988) Proc. Natl. Acad. Sci. USA 85:5879-5883). Such single chain antibodies are also intended to be encompassed within the term "antigen—binding fragment" of an antibody. Furthermore, the antigen- binding fragments include binding-domain immunoglobulin fusion proteins comprising (i) a binding domain polypeptide (such as a heavy chain variable region, a light chain variable region, or a heavy chain variable region fused to a light chain variable region via a linker peptide) that is fused to an immunoglobulin hinge region polypeptide, (ii) an immunoglobulin heavy chain CH2 constant region fused to the hinge region, and (iii) an immunoglobulin heavy chain CH3 constant region fused to the CH2 constant region. The hinge region may be modified by ing one or more cysteine residues with serine es so as to prevent dimerization. Such binding-domain immunoglobulin fusion ns are further disclosed in US 2003/0118592 and US 2003/0133939. These dy fragments are ed using conventional techniques known to those with skill in the art, and the fragments are screened for utility in the same manner as are intact antibodies.

The term ized antibody” encompasses futiy humanized antibody (i.e., frameworks are 100% humanized) and partially humanized antibody (e.g., at least one variable domain contains one or more amino acids from a human antibody, while other amino acids are amino acids of a non—human parent antibody). Typically a “humanized antibody” contains CDRs of a man parent antibody (e.g., mouse, rat, rabbit, man primate, etc.) and frameworks that are cal to those of a natural human antibody or of a human antibody consensus. In such instance, those ized antibodies" are characterized as fuiiy humanized. A “humanized antibody” may also contain one or more amino acid substitutions that have no correspondence to those of the human antibody or human antibody consensus. Such substitutions include, for example, back-mutations (e.g., roduction of non-human amino acids) that may preserve the antibody characteristics (e.g., affinity, specificity etc.). Such substitutions are usually in the framework region. A “humanized antibody” optionaIiy also comprise at least a portion of a constant region (Fc) which is typically that of a human antibody. Typically, the nt region of a “humanized antibody” is identical to that of a human antibody.

The term “natural human antibody” refers to an antibody that is encoded (encodable) by the human antibody repertoire, i.e., germline sequence.

The term ric antibody” refers to an antibody having non-human variable region(s) and human constant .

The term “hybrid antibody” refers to an antibody comprising one of its heavy or light chain variable region (its heavy or light chain) from a certain types of antibody (e.g., humanized) while the other of the heavy or light chain variable region (the heavy or light chain) is from another type (e.g., murine, ic).

In some embodiments, the heavy chain and/or light chain framework region of the humanized antibody may comprises from one to thirty amino acids from the non— human antibody which is sought to be humanized and the remaining portion being from a natural human dy or a human antibody consensus. In some instances, the humanized antibody may comprise from 1 to 6 non-human CDRs and often the six CDRs are non-human.

The natural human dy selected for zation of the non—human parent antibody may comprise a variable region having a three-dimensional structure similar to that of (superimposable to) a (modeled) variable region of the non—human parent antibody. As such, the zed antibody has a greater chance of having a three- dimensional ure similar to that of the non—human parent antibody.

The light chain variable region of the natural human antibody selected for humanization purposes, may have, for example an overall (over the entire light chain le region) of at least 70%, 75%, 80%, etc. identity with that of the non-human parent antibody. Alternatively, the light chain framework region of the natural human dy selected for humanization purposes, may have, for example, at least 70% 75%, 80%, 85% etc. sequence identity with the light chain framework region of the non-human parent antibody. In some embodiments, the natural human antibody selected for humanization purposes may have the same or substantially the same number of amino acids in its light chain complementarity determining region to that of a light chain complementarity determining region of the non-human parent antibody.

The heavy chain variable region of the l human antibody selected for humanization purposes, may have, for example an overall (over the entire heavy chain variable region) of at least 60%, 70%, 75%, 80%, etc. identity with that of the non-human parent antibody. Also in accordance with the t invention, the human framework region amino acid residues of the humanized antibody heavy chain may be from a l human antibody heavy chain framework region having at least 70%, 75%, 89% etc. identity with a heavy chain framework region of the non- human parent antibody. In some embodiments, the natural human antibody selected for humanization purposes may have the same or substantially the same number of amino acids in its heavy chain mentarity ining region to that of a heavy chain complementarity determining region of the non-human parent antibody.

The natural human antibody that is selected for zation of the non-human parent antibody may comprise a variable region having a three-dimensional structure similar to that of (superimposable to) a (modeled) variable region of the non-human parent antibody. As such, the humanized or hybrid antibody has a greater chance of having a three-dimensional structure similar to that of the non—human parent antibody.

For example, the natural human dy heavy chain variable region which may be selected for humanization purposes may have the ing characteristics: a) a three—dimensional structure similar to or identical imposable) to that of a heavy chain of the non-human antibody and/or b) a framework region having an amino acid sequence at least 70% identical to a heavy chain framework region of the non-human antibody. Optionally, (a number of) amino acid residues in a heavy chain CDR (e.g., all three CDRs) is the same or substantially the same as that of the non-human heavy chain CDR amino acid residues.

Alternatively, the l human antibody light chain variable region which may be selected for humanization purposes may have the ing teristics: a) a three-dimensional structure similar to or identical (superimposable) to that of a light chain of the non—human antibody, and/or b) a framework region having an amino acid sequence at least 70% identical to a light chain framework region of the non-human antibody. Optionally, (a number of) amino acid residues in a light chain CDR (e.g., all three CDRs) that is the same or substantially the same as that of the non-human light chain CDR amino acid residues.

A typical antigen binding site is comprised of the variable regions formed by the pairing of a light chain immunoglobulin and a heavy chain immunoglobulin. The structure of the antibody variable regions is very consistent and exhibits very similar structures. These variable regions are typically sed of relatively homologous framework regions (FR) interspaced with three hypervariable regions termed Complementarity Determining Regions (CDRs). The overall binding activity of the antigen binding fragment is often dictated by the sequence of the CDRs. The FRs often play a role in the proper positioning and alignment in three dimensions of the CDRs for optimal antigen binding.

Antibodies and/or antigen binding fragments of the t invention may originate, for example, from a mouse, a rat or any other mammal or from other s such as through recombinant DNA technologies.

In another aspect, the invention provides an antibody or antigen binding fragment f e of specific binding to Kidney associated n 1 (KAAG1) having a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO.:5, SEQ ID NO.:6 and SEQ ID NO.:7 and a light chain variable region comprising the amino acid ce set forth in SEQ ID NO.: 8, SEQ ID NO.:9 and SEQ ID NO.:10.

In another aspect, the invention provides an antibody or antigen binding fragment thereof that specifically binds to kidney associated antigen 1 (KAAG1) and has an ty constant (KD) of less than 10 picomolar.

In another aspect, the invention provides an antibody or antigen binding fragment thereof capable of competing with the antibody or antigen binding fragment thereof of the invention and having an affinity constant (KD) of less than 1nM, provided that said antibody is not the 3G10 antibody or antigen binding fragment thereof.

In r aspect, the invention provides a nucleic acid encoding a light chain variable region and/or a heavy chain variable region of the antibody or antigen binding fragment of the invention.

In another aspect, the invention provides a vector comprising the c acid of the invention.

In another , the invention provides an ed cell comprising the nucleic acid of the invention, the vector of the invention or the antibody or antigen binding nt of the invention.

In another aspect, the ion provides a pharmaceutical composition comprising the antibody or antigen binding fragment of the ion, and a pharmaceutically acceptable carrier.

In r aspect, the ion provides a method for detecting KAAG1 or a KAAG1 variant, the method comprising contacting an isolated cell expressing KAAG1 or the KAAG1 variant or a sample comprising or suspected of comprising KAAG1 or the KAAG1 variant with the antibody or n binding fragment thereof of the invention and measuring binding.

In another aspect, the invention provides a composition comprising the antibody or antigen binding nt of the invention, and a carrier.

In another aspect, the invention provides a kit comprising the antibody or antigen binding fragment of the ion.

In another , the invention provides use of the dy or antigen binding fragment of the invention, the pharmaceutical composition of the invention or the composition of the ion in the manufacture of a medicament for detection, diagnosis or treatment of cancer comprising cells expressing KAAG1 or a KAAG1 variant.

In another aspect, the ion provides use of the antibody or antigen binding fragment of the invention, the pharmaceutical composition of the invention or the composition of the ion in the detection of a tumor ex vivo or in the diagnosis of cancer ex vivo, wherein the tumor or cancer comprises cells expressing KAAG1 or a KAAG1 variant.

In another aspect, the invention provides a method for obtaining an antibody or antigen binding fragment thereof, suitable for use as an antibody-drug conjugate for the treatment of cancer comprising cells sing KAAG1 or a KAAG1 variant, the method comprising: a. providing an antibody or antigen binding fragment thereof which specifically binds to an epitope comprised between amino acids 61 and 84 of KAAG1 or a KAAG1 variant; b. testing alization of the antibody or antigen binding fragment within an isolated cell expressing KAAG1 or a KAAG1 variant or in a non-human animal and; c. isolating an antibody or an antigen binding nt which is internalized.

In another aspect, the invention provides a method of making the antibody or antigen binding fragment thereof of the invention, sing culturing an isolated cell of the invention so that the antibody or n g fragment f is produced.

Further scope, ability and advantages of the present invention will become apparent from the non-restrictive detailed description given after. It should be understood, however, that this detailed description, while ting exemplary embodiments of the invention, is given by way of example only, with reference to the accompanying drawings.

Any discussion of documents, acts, als, devices, articles or the like which has been included in the present specification is not to be taken as an admission that any or all of these matters form part of the prior art base or were common general knowledge in the field relevant to the present disclosure as it existed before the priority date of each claim of this application.

Throughout this specification the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the ion of a stated t, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 shows the results from the ELISA that compares the binding of the 3A4 chimeric anti-KAAG1 antibody with a control antibody when incubated with increasing concentrations of recombinant human KAAG1. The binding curve of 3A4 is shown by the lighter colored line.

Figure 2 shows a histogram that describes the results from ELISA analyses to map the epitope specificity of the 3A4 anti-KAAG1 antibody. The results showed that 3A4 interacted with a sequence of amino acids contained in the carboxy-terminus of KAAG1 between amino acids 61 – 84. The binding of 3A4 was compared with 3C4, 3D3, and 3G10 anti-KAAG1 antibodies that were known to interact with regions 1 – , 36 – 60, and 61 – 84 of KAAG1, tively.

Figure 3A shows the results of flow cytometry performed on SKOV-3 and TOV-21G ovarian cancer cells with the 3A4 anti-KAAG1 antibody (darker line) compared with a control IgG (lighter line).

Figure 3B shows the results of flow cytometry med on 293E human kidney cells with the 3A4 anti—KAAG1 antibody r line) compared with a control lgG (lighter line).

Figure 4 represents the detection of the KAAG1 antigen on the surface of SKOV-3 cells by flow cytometry with the 3A4 anti-KAAG1 antibody. The fluorescence signal decreases with time when the cells were incubated at 37 C, which suggests that the antibody complex was internalized during the incubation when the cells were incubated with 3A4.

Figure 5 shows the internalization of 3A4 anti-KAAG1 antibody and its co-localization with LAMP1, a protein associated with endosomal and lysosomal membranes.

Figure 6A and BB are graphs representing FACs analysis of tumor cells exposed to different anti—KAAG1 antibodies.

Figure 7 are schematics enting 2 likely representation of the KAAG1 orientation in the cell membrane.

Figure 8 is a molecular model n diagram) of the murine 3A4 variable domain.

CDR loops are colored in black and labelled L1. L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray.

Figure 9a is a molecular models of humanized antibody Lh1Hh1 (i.e., humanized light chain 1 and humanized heavy chain 1) of 3A4 variable domains. CDR loops are colored in black and ed L1, L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray. The side-chains of residues mutated from murine framework to human framework are rendered in ball-and-stick representation. Lh1 ated the humanized light chain of variant 1 and HM designated the heavy chain of variant 1.

Figure 9b is a molecular models of humanized antibody Lh1Hh2 (i.e., humanized light chain 1 and humanized heavy chain 2) of 3A4 variable domains. CDR loops are colored in black and labelled L1, L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray. The side-chains of residues mutated from murine framework to human framework are rendered in nd-stick entation. Lh1 designated the humanized light chain of variant 1 and Hh2 designated the heavy chain of variant 2.

Figure 9c is a molecular models of humanized antibody Lh1Hh3 (i.e., humanized light chain 1 and humanized heavy chain 3) of 3A4 le domains. CDR loops are colored in black and labelled L1, L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray. The side—chains of residues mutated from murine ork to human framework are rendered in ball-and-stick representation. Lh1 designated the humanized light chain of variant 1 and Hh3 designated the heavy chain of variant 3.

Figure 9d is a molecular models of humanized antibody Lh1Hh4 (i.e., humanized light chain 1 and humanized heavy chain 4) of 3A4 variable domains. CDR loops are colored in black and labelled L1, L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray. The side-chains of residues mutated from murine framework to human framework are rendered in ball-and-stick entation. Lh1 designated the zed light chain of variant 1 and HM designated the heavy chain of variant 4.

Figure 9e is a molecular models of humanized antibody Lh2Hh1 (i.e., humanized light chain 2 and humanized heavy chain 1) of 3A4 variable domains. CDR loops are colored in black and labelled L1, L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray. The side-chains of residues mutated from murine framework to human framework are ed in nd-stick representation. Lh2 designated the humanized light chain of variant 2 and HM designated the heavy chain of variant 1.

Figure 9f is a molecular models of humanized antibody Lh2Hh2 (i.e., humanized light chain 2 and humanized heavy chain 2) of 3A4 variable domains. CDR loops are colored in black and ed L1, L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray. The side-chains of residues mutated from murine framework to human framework are rendered in ball-and-stick representation. Lh2 designated the humanized light chain of variant 2 and Hh2 designated the heavy chain of variant 2.

Figure 99 is a molecular models of humanized antibody Lh2Hh3 (i.e., humanized light chain 2 and zed heavy chain 3) of 3A4 variable domains. CDR loops are colored in black and ed L1, L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray. The side-chains of residues mutated from murine ork to human framework are rendered in ball-and-stick representation. Lh2 designated the humanized light chain of variant 2 and Hh3 designated the heavy chain of variant 3.

Figure 9h is a molecular models of humanized antibody Lh2Hh4 (i.e., humanized light chain 2 and humanized heavy chain 4) of 3A4 variable domains. CDR loops are d in black and labelled L1, L2 and L3 in the light chain and H1, H2 and H3 in the heavy chain. The framework region is shown in gray. The side-chains of residues mutated from murine ork to human framework are rendered in ball-and-stick representation. Lh2 designated the humanized light chain of variant 2 and HM designated the heavy chain of variant 4.

Figure 10a is an amino acid sequence alignment of the 3A4 variable domains of the murine and humanized light chains. The light chain has two humanized variants (Lh1 an Lh2). The CDRs are shown in bold and indicted by CDRL1, CDRL2 and CDRL3.

Back ons in the human framework regions that are murine amino acids are underlined in the humanized sequences.

Figure 10b is an amino acid ce alignment of the 3A4 variable s of the murine and humanized heavy chains. The heavy chain has four zed variants (Hh1 to Hh4). The CDRs are shown in bold and indicted by CDRH1, CDRH2 and CDRH3. Back mutations in the human framework regions that are murine amino acids are underlined in the humanized sequences.

Figure 11A is an ent of murine 3A4 light chain variable region (SEQ ID NO.:4) with a light chain variable region variant (SEQ ID NO.:33) using the ClustalW2 program (Larkin M.A., et al., (2007) ClustalW and ClustalX version 2. Bioinformatics 2007 : 2947-2948) where an “*" (asterisk) indicates positions which have a single, fully conserved residue, wherein (colon) indicates conservation between groups of strongly similar properties - scoring > 0.5 in the Gonnet PAM 250 matrix and where (period) indicates conservation between groups of weakly similar properties - g =< 0.5 in the Gonnet PAM 250 matrix.

Figure 113 is an alignment of murine 3A4 heavy chain variable region (SEQ ID N02) with a light chain variable region variant (SEQ ID NO.:38) using the ClustalW2 m (Larkin M.A., et al., (2007) ClustalW and ClustalX version 2. Bioinformatics 2007 23(21): 2947-2948) where an “*” (asterisk) indicates positions which have a single, fully ved residue, wherein (colon) indicates conservation between groups of strongly similar properties - scoring > 0.5 in the Gonnet PAM 250 matrix and where (period) indicates conservation between groups of weakly r properties - scoring =< 0.5 in the Gonnet PAM 250 .

Figure 12a represents plasmid Map of pKCR5—3A4-HC-Variant 1. The heavy chains of the humanized 3A4 variants were cloned in the same manner into the Hindlll site of pK-CR5. Consequently the resulting plasmids are identical to 3A4-HC variant 1 except for the sequence of the heavy chain immunoglobulin variable domain.

Figure 12b represents plasmid Map of pMPG—CR5-3A4-LC-Variant 1. The light chains of the humanized variants 1 and 2 of 3A4 antibody were cloned in the same manner into the BamHl site of pMPG-CRS. Consequently, the resulting plasmid is identical to pMPG-CR5-3A4-LC-Variant 1, except for the sequence of the light chain immunoglobulin variable domain.

Figure 13 represents an analysis of antibody tion after transient transfection in CHO cells. Supernatant (13 days post—transfection) of CHOcTA cells transfected with the different combinations of light and heavy chains of zed 3A4 antibody were analyzed by western blot. Quantification of antibody produced in the atants was determined after scanning the bands of the western blot t dilution of a known standard (human purified lgG antibody). Mr molecular weight marker (kDa).

Figure 14 is a graph of a ex GT5 gel filtration of recombinant KAAG1 sample.

KAAG1 was injected over the gel filtration and separated at 0.4 ml/min. The largest peak between fractions 15 — 19.

Figure 15 is a Table listing the rate and affinity constants for the murine and humanized variants of the 3A4 antibody.

Figure 16A is an histogram illustrating the association rates (Ka) of the humanized dies.

Figure 163 is an histogram rating the dissociation rates (Kd) of the humanized antibodies.

Figure 16C is an histogram illustrating the affinity constants (KB) of the humanized antibodies.

Figure 17a illustrates humanized 3A4 variants binding to KAAG1 in an ELISA. This figure shows the comparative binding of 3A4 humanized antibody variants and the murine 3A4. Concentration-dependent binding profiles of the humanized heavy chains (Hh1, Hh2, Hh3 and HM) assembled with the Lh1 light chain variant.

Figure 17b illustrates humanized 3A4 variants binding to KAAG1 in an ELISA. This figure shows the comparative binding of 3A4 humanized antibody variants and the murine 3A4. Concentration-dependent binding profiles of the humanized heavy chains (Hh1, Hh2, Hh3 and HM) assembled with the Lh2 light chain variant.

Figure 18 illustrates humanized 3A4 ts binding to KAAG1 on the surface of cancer cells. This illustration shows the comparative binding activity of the humanized and the murine 3A4 antibodies on the unpermeabilized SKOV—3 ovarian cancer cells.

DETAILED PTION OF THE INVENTION The expression and biological activity of KAAG1 in cancer cells The present invention relates to the use of antibodies to target tumors found in various cancer types, in particular n cancer. In order to direct the antibodies to the , the identification of tumor-specific antigens that are expressed at the cell surface of the cancer cells must be carried out. There are several technologies that are available to identify tumor-specific ns and the method that was used to identify KAAG1 in ovarian tumors, an innovative discovery platform called Subtractive Transcription-based ication of mRNA (STAR), is described in the published patent application No. published under No. WO/2007/147265 on December 27, 2007.

Analysis of the ovarian cancer STAR libraries yielded many genes that encode secreted and cell e proteins. One of these, termed AB-0447, contained an open reading frame that d a polypeptide of 84 amino acids, ponding to SEQ ID NO.:29 that was encoded by a cDNA of 885 base pairs with the nucleotide sequence shown in SEQ ID NO.:28. A search of publicly available databases revealed that the AB-0447 nucleotide ce was identical to that of a gene called KAAG1. Bioinformatic analysis ted a membrane-anchored protein that presents its functional domain to the extracellular tment. KAAG1 was originally cloned from a kidney cancer library as a cell surface antigen, a result that confirms its membrane zation. Additionally, our studies showed that the protein was processed at its amino-terminus, a result that was consistent with ge of a functional signal peptide at or between amino acids 30 and 34. Furthermore, transient sion of the full-length cDNA resulted in detection of cleaved KAAG1 in the culture medium. This last finding indicated that this membrane-anchored protein could be shed from the cells when sed at high levels. in contrast, expression of an amino-truncated mutant of KAAG1 resulted in intra-cellular retention of the protein.

There are currently no published reports that shed any light on its function and the over-expression of KAAG1 in ovarian cancer, as disclosed by this invention, has never been usly documented.

We have thus investigated whether KAAG1 could be used for antibody-based diagnostics and therapeutics.

Several ovarian cancer cell-based models have been established, such as TOV-21G, TOV-112D, OV-90, and others, and are ar to those skilled in the art. These cells are part of a collection of human ovarian cancer cell lines d from patients with ovarian tumors or ascites fluid. These cell lines have undergone an in-depth analysis, including global gene expression patterns on rrays that make them excellent cell-based models for human ovarian cancer. The growth properties, gene expression patterns, and response to chemotherapeutic drugs indicated that these cell lines are very representative of ovarian tumor behavior in vivo (Benoit et al., 2007). RT-PCR analysis of total RNA isolated from these ovarian cancer cell lines showed that the KAAG1 transcript was weakly expressed in the cell lines derived from primary tumors. in contrast, cell lines d from ascitic fluid contained high levels of KAAG1 expression. The increased expression of KAAG1 in cells from the ascitic fluid suggested that the nment of the cells influences the regulation of the KAAG1 gene. c cells are associated with advanced disease and this pattern of expression implies that increased KAAG1 levels are associated with anchorage- independent growth. In concordance with this latter suggestion, KAAG1 expression was found to significantly increase in cell lines derived from primary tumors when these cells were cultured as spheroids in 3D cultures. These spheroids have been extensively terized and were found to y many properties associated with tumors in vivo (Cody et al., 2008). Thus, expression of KAAG1 was found to be significantly increased in models that mimic tumor progression, in particular during the evolution of ovarian cancer.

With the demonstration that KAAG1 expression is ted in ovarian cancer cells, the function of this gene in ovarian cancer cell behavior was examined in cell-based assays. To that effect, RNA interference (RNAi) was used to knock down the expression of the endogenous KAAGi gene in the ovarian cancer cell lines and it was found that decreased expression of KAAG1 resulted in a significant reduction in the migration of the cells as ined in a standard cell ty assay, as exemplified by a wound healing (or h) assay. This type of assay measures the speed at which cells fill a denuded area in a confluent monolayer. sed expression of KAAG1 resulted in a reduction in the survival of ovarian cancer cell lines as ed by a clonogenic assay, such as a colony survival assay. Those skilled in the art may use other methods to evaluate the requirement of KAAG1 in the behavior of cancer cells, in particular ovarian cancer cells.

Based on the expression of KAAG1 in a large proportion of ovarian tumors, its limited expression in normal tissues, and a concordance between expression levels and increased malignancy, and a ve biological role for KAAG1 in the behavior of n cancer cell lines, KAAG1 was chosen as a therapeutic target for the development of antibodies for the detection, prevention, and treatment of n cancer. Expression of KAAG1 in cancers, other than ovarian cancer also lead the Applicant to the evaluation of eutic or diagnostic antibodies for other cancer indications.

The present invention therefore provides anti-KAAG1 antibodies and antigen binding fragments thereof which specifically target KAAG1 and which may be used, for example, as an antibody-drug conjugate.

Such antibodies and antigen binding fragments include for example, onal antibodies, polyclonal antibodies, chimeric antibodies, humanized antibodies, dy fragments, single chain antibodies, domain antibodies, and polypeptides having an antigen binding region.

Antibodies and_antigen binding fragments that binds to KAAG1 Antibodies were initially ed from Fab librairies for their specificity towards the antigen of interest.

The le regions of the antibodies or antigen binding fragments described herein may be fused with constant regions of a desired species thereby aliowing recognition of the dy by effector cells of the desired species. The constant region may originate, for example, from an IgG1, IgG2, lgG3, or lgG4 subtype. Cloning or synthesizing a constant region in frame with a variable region is well within the scope of a person of skill in the art and may be performed, for example, by recombinant DNA technology. Thus, antibodies comprising constant region of a human antibody as well as antibodies or antigen binding fragments comprising framework amino acids of a human antibody are also encompassed by the present invention.

The present invention therefore es in an exemplary embodiment, an isolated antibody or antigen binding fragment comprising a light chain variable region having; a. a CDRL1 sequence comprising SEQ ID NO.:8 or as set forth in SEQID NO.:8; b. a CDRL2 sequence comprising SEQ ID NO.:9 or as set forth in SEQ ID NO.:9, or; c. a CDRL3 sequence comprising SEQ ID NO.:10 or as set forth in SEQ ID NO.:10.

The isolated antibody or antigen binding fragment may also comprise a heavy chain variable region ; a. a CDRH1 sequence comprising SEQ ID NO.:5 or as set forth in SEQ ID NO.:5; b. a CDRH2 sequence comprising SEQ ID NO.:6 or as set forth in SEQ ID NO.:6, or; c. a CDRH3 sequence comprising SEQ ID N02? or as set forth in SEQ ID NO.:7.

In an exemplary embodiment, the antibody or antigen binding fragment may se any individual CDR or a combination of CDR1, CDR2 and/or CDR3 of the light chain variable region. The CDR3 may more particularly be selected. Combination may include for example, CDRL1 and CDRL3; CDRL1 and CDRL2; CDRL2 and CDRL3 and; CDRL1, CDRL2 and CDRL3.

In another exemplary embodiment, the antibody or antigen g fragment may comprise any dual CDR or a ation of CDR1, CDR2 and/or CDR3 of the heavy chain variable region. The CDR3 may more ularly be selected. ation may include for example, CDRH1 and CDRH3; CDRH1 and CDRH2; CDRH2 and CDRH3 and; CDRH1, CDRH2 and CDRH3.

In accordance with the present invention, the antibody or antigen binding fragment may comprise at least two CDRs of a CDRL1, a CDRL2 or a CDRL3.

Also in accordance with the present invention, the antibody or antigen binding fragment may comprise one CDRL1, one CDRL2 and one CDRL3.

Further in accordance with the present invention, the antibody or n binding nt may comprise: a. At least two CDRs of a CDRL1, CDRL2 or CDRL3 and; b. At least two CDRs of a CDRH1, one CDRH2 or one CDRH3.

The antibody or n binding fragment may more preferably comprise one CDRL1, one CDRL2 and one CDRL3.

The dy or antigen g fragment may also more preferably comprise one CDRH1, one CDRH2 and one CDRH3.

In accordance witht the present invention, the antibody or antigen binding fragment may comprise one CDRH1, one CDRH2 or one CDRH3.

In accordance witht the t invention, the antibody or antigen binding fragment may also comprise one CDRH1, one CDRH2 and one CDRH3.

When only one of the light chain variable region or the heavy chain variable region is available, an antibody or antigen-binding fragment may be reconstituted by screening a library of complementary variable regions using methods known in the art (Portolano et al. The Journal of Immunology (1993) 150:880-887, Clarkson et al., Nature (1991) 352:624-628).

Also encompassed by the present invention are polypeptides or antibodies comprising variable chains having at least one conservative amino acid substitution in at least one of the CDRs bed herein (in comparison with the original CDR).

The present invention also encompasses polypeptides or antibodies sing le chains having at least one conservative amino acid substitution in at least two of the CDRs (in comparison with the original CDRs).

The present invention also encompasses polypeptides or antibodies comprising le chains having at least one conservative amino acid substitution in the 3 CDRs (in ison with the original CDRs).

The present invention also encompasses polypeptides or antibodies comprising variable chains having at least two conservative amino acid substitutions in at least one of the CDRs (in comparison with the original CDRs).

The present invention also encompasses polypeptides or dies comprising le chains having at least two conservative amino acid substitutions in at least two of the CDRs (in comparison with the original CDRs).

The present invention also encompasses polypeptides or antibodies comprising variable chains having at least two conservative amino acid substitutions in the 3 CDRs (in ison with the original CDRs).

In another aspect, the present invention relates to a polypeptide, antibody or antigen binding fragment comprising (on a single polypeptide chain or on separate ptide chains) at least one cornplementarity-determining region of a light chain variable region and at least one complementarity—determining region of a heavy chain variable region of one of the antibodies or antigen binding fragment bed .

The present ion relates in another aspect thereof to anti-KAAG1 antibodies that may comprise (on a single polypeptide chain or on separate polypeptide chains) all six complementarity—determining regions (CDRs) of the antibody or antigen binding fragment described herein.

Variant antibody and antigen binding fragments The present invention also encompasses variants of the antibodies or antigen binding fragments described herein. t antibodies or antigen binding fragments included are those having a ion in the amino acid sequence. For example, variant antibodies or n binding nts included are those having at least one variant CDR (two, three, four, five or six variant CDRs or even twelve variant CDRs), a variant light chain variable region, a variant heavy chain variable region, a variant light chain and/or a t heavy chain. Variant antibodies or antigen binding fragments ed in the present invention are those having, for example, similar or improved binding affinity in comparison with the original antibody or antigen binding fragment.

As used herein the term “variant” s to any of the ce described herein and includes for example, a variant CDR (either CDRL1, CDRLZ, CDRL3, CDRH1, CDRH2 and/or CDRH3), a variant light chain variable region, a t heavy chain variable region, a variant light chain, a variant heavy chain, a t antibody, a variant antigen binding fragment and a KAAG1 variant.

Variant antibodies or n binding fragments encompassed by the present invention are those which may comprise an insertion, a deletion or an amino acid substitution (conservative or non—conservative). These variants may have at least one amino acid residue in its amino acid sequence removed and a different residue inserted in its place.

The antibody or antigen g fragment of the present invention may have a light chain variable region and/or heavy chain variable region as described above and may further comprise amino acids of a constant region, such as, for example, amino acids of a constant region of a human antibody.

In an exemplary embodiment, the antibody or antigen binding fragment of the present invention may comprise, for example, a human lgG1 constant region.

In accordance with r exemplary embodiment of the invention, the antigen binding fragment may be, for e, a scFv, a Fab, a Fab' or a (Fab'); A site of interest for substitutional mutagenesis includes the hypervariable regions (CDRs), but cations in the framework region or even in the constant region are also contemplated. Conservative substitutions may be made by exchanging an amino acid (of a CDR, variable chain, antibody, etc.) from one of the groups listed below (group 1 to 6) for another amino acid of the same group.

Other exemplary embodiments of conservative substitutions are shown in Table 1A under the heading of “preferred substitutions“. If such tutions result in a undesired property, then more substantial changes, denominated "exemplary substitutions" in Table 1A, or as further bed below in reference to amino acid classes, may be introduced and the products screened.

It is known in the art that variants may be generated by substitutional mutagenesis and retain the biological activity of the polypeptides of the present invention. These variants have at least one amino acid residue in the amino acid ce removed and a different residue inserted in its place. For example, one site of interest for substitutional mutagenesis may include a site in which particular residues obtained from various species are identical. Examples of substitutions identified as “conservative substitutions” are shown in Table 1A. If such substitutions result in a change not d, then other type of substitutions, denominated “exemplary tutions" in Table 1A, or as further described herein in reference to amino acid classes, are introduced and the products screened.

Substantial modifications in function or immunological identity are accomplished by selecting tutions that differ significantly in their effect on maintaining (a) the structure of the polypeptide backbone in the area of the substitution, for e, as a sheet or helical conformation. (b) the charge or hydrophobicity of the molecule at the target site, or (c) the bulk of the side chain. Naturally occurring residues are divided into groups based on common side chain properties: (group 1) hydrophobic: norleucine, methionine (Met), Alanine (Ala), Valine (Val), e (Leu), lsoieucine (lle) (group 2) neutral hydrophilic: ne (Cys), Serine (Ser), Threonine (Thr) (group 3) acidic: Aspartic acid (Asp), Glutamic acid (Glu) (group 4) basic: Asparagine (Asn), Glutamine (Gln), Histidine (His), Lysine (Lys), ne (Arg) (group 5) residues that influence chain orientation: Glycine (Gly), Proline (Pro); and (group 6) aromatic: Tryptophan (Trp), Tyrosine (Tyr), Phenylalanine (Phe) Non-conservative substitutions will entail exchanging a member of one of these classes for another.

Table 1A. Amino acid substitution Original residue 7 Exemplary substitution Conservative substitution Ala (A) lVal, Leu, lle Val Arg (R) ‘ Lys, Gln, Asn Lys Asn (N) lGln, His, Lys, Arg, Asp Gln IEys (C)Asp (D) , AlaGlu, Asn Glu JSer Original residue Exemplary substitution Conservative substitution lle(| Leu, Val, Met, Ala, Phe, norieucine Norleucine, lle, Val, Met, lle Ala, Phe Arg, Gln, Asn Arg Leu, Phe, lle Leu Leu, Val, lle, Ala, Tyr Tyr Ser (8) Thr Thr (T) Ser Trp (W) Tyr, Phe Tyr -norieucineVal (V) lle, Leu, Met, Phe, Ala, Variant antibody or antigen binding fragment may have substantial sequence similarity and/or ce ty in its amino acid ce in comparison with that the original antibody or antigen binding fragment amino acid sequence. The degree of similarity between two ces is based upon the percentage of identities (identical amino acids) and of conservative substitution.

Generally, the degree of similarity and ty between variable chains has been determined herein using the Blast2 ce program (Tatiana A. Tatusova, Thomas L. Madden (1999), "Blast 2 sequences - a new tool for comparing protein and nucleotide sequences", FEMS Microbiol Lett. 174:247-250) using default settings, i.e., blastp program, BLOSUMBZ matrix (open gap 11 and extension gap penalty 1; gapx dropoff 50, expect 10.0, word size 3) and activated filters.

Percent identity will therefore be indicative of amino acids which are identical in ison with the original peptide and which may occupy the same or similar position.

Percent similarity will be indicative of amino acids which are identical and those which are replaced with conservative amino acid substitution in comparison with the original peptide at the same or similar position.

Variants of the present invention therefore comprise those which may have at least 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence identity with an original sequence or a portion of an original sequence.

Exemplary embodiments of variants are those having at least 81% sequence identity to a sequence described herein and 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% ce similarity with an original sequence or a portion of an original sequence.

Other exemplary embodiments of variants are those having at least 82% sequence identity to a sequence described herein and 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence similarity with an al ce or a portion of an original sequence. r exemplary embodiments of variants are those having at least 85% sequence identity to a sequence described herein and 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence similarity with an al sequence or a portion of an original sequence.

Other exemplary embodiments of variants are those having at least 90% sequence identity to a sequence bed herein and 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence similarity with an original sequence or a portion of an original sequence.

Additional ary embodiments of ts are those having at least 95% sequence ty to a sequence described herein and 95%, 96%, 97%, 98%, 99% or 100% sequence similarity with an original sequence or a portion of an original sequence.

Yet additional exemplary embodiments of variants are those having at least 97% sequence identity to a sequence described herein and 97%, 98%, 99% or 100% sequence similarity with an original sequence or a n of an original sequence.

For a e of concision the applicant provides herein a Table IB illustrating exemplary embodiments of individual variants encompassed by the present invention and comprising the ied % ce identity and % sequence rity. Each “X” is to be construed as defining a given variant.

Table 1 B t (%) sequence identity -m--m-----mmmmmmmmm X X -------------- --------------—------ -------------------- g 84 X --------------- E X X X - - - a X X X IX - II.- 3 x x x x x g x x x x x x x 3 x x x x x x x x - 3 x x x x x x x x x x § x x x x x x x x x x x '-- L; x x x x x x x x x x x 8 x x x x x x x x x x x I a T— x x x x x x x x x x x x a x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x |x x x x J x \x g x x x x x x X x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x The present invention encompasses CDRs, light chain variable regions, heavy chain variable regions, light , heavy chains, antibodies and/or antigen binding fragments which comprise at least 80% identity with the sequence described .

Exemplary embodiments of the antibody or antigen binding fragment of the present invention are those comprising a light chain variable region comprising a sequence at least 70%, 75%, 80% identical to SEQ ID NO.:4.

These light chain variable region may comprise a CDRL1 sequence at least 80 % identical to SEQ ID NO.:8, a CDRL2 sequence at least 80 % identical to SEQ ID NO.:9 and a CDRL3 sequence at least 80 % identical to SEQ ID NO.:10.

In an exemplary ment of the present invention, any of the antibodies provided herein may comprise a CDRL1 sequence which may be at least 90 % identical to SEQ ID NO.:8.

In another exemplary embodiment of the present invention, any of the antibodies provided herein may comprise a CDRL1 sequence which may be 100% cal to SEQ ID NO.:8.

In another exemplary embodiment of the present invention, any of the antibodies provided herein may comprise a CDRL2 ce at least 90 % cal to SEQ ID NO.:9.

In yet another exemplary embodiment of the present invention, any of the antibodies provided herein may comprise a CDRL2 sequence which may be 100% identical to SEQ ID NO.:9.

In another exemplary embodiment of the present invention, any of the antibodies provided herein may comprise a CDRL3 sequence which may be at least 90 % identical to SEQ ID NO.:10.

In an additional exemplary ment of the present invention, any of the antibodies provided herein may comprise a CDRL3 sequence which may be 100% identical to SEQ ID NO.:10.

In an exemplary ment, the antibody or n binding fragment may comprise a heavy chain variable region comprising a ce at least 70%, 75%, 80% identical to SEQ ID NO.:2.

These heavy chain variable regions may comprise a CDRH1 sequence at least 80 % cal to SEQ ID NO.:5, a CDRH2 sequence at least 80 % identical to SEQ ID NO.:6 and a CDRH3 sequence at least 80 % identical to SEQ ID NO.:7.

In an exemplary embodiment of the present invention, any of the antibodies provided herein may comprise a CDRH1 sequence which may be at least 90 % identical to SEQ ID N05.

In another exemplary embodiment of the present invention, any of the dies provided herein may comprise a CDRH1 sequence which may be 100% identical to SEQ ID NO.:5.

In yet another exemplary embodiment of the present invention, any of the antibodies provided herein may comprise a CDRH2 sequence which may be at least 90 % identical to SEQ ID N056.

In a further exemplary embodiment of the present invention, any of the antibodies provided herein may comprise a CDRH2 sequence which may be 100% identical to SEQ ID NO.:6.

In yet a further exemplary embodiment of the t invention, any of the antibodies provided herein may comprise a CDRH3 sequence which may be at least 90 % identical to SEQ ID NO.:7.

In an onal exemplary embodiment of the present invention, any of the antibodies provided herein may comprise a CDRH3 sequence which may be 100% identical to SEQ ID NO.:7.

In some ces, the variant antibody heavy chain variable region may comprise amino acid deletions or additions (in combination or not with amino acid substitutions). Often 1, 2, 3, 4 or 5 amino acid deletions or additions may be tolerated.

Exemplary ments of variant antibody or antigen g fragments include those having a light chain le region as set forth in SEQ ID NO.:30: SEQ ID NO.:30 DXVMTQTPLSLXVXXGXXASlSCRSSQSLLHSNGNTYLEWYLQKPGQSPXLLIHTVS NRFSGVPDRFSGSGSGTDFTLKISRVEAEDXGWYCFQGSHVPLTFGXGTXLEXK, wherein at least one of the amino acids identified by X is an amino acid substitution (conservative or non-conservative) in ison with a corresponding amino acid in the polypeptide set forth in SEQ ID NO.:4. The amino acid substitution may be, for example, an amino acid found at a corresponding position of a natural human antibody or a human antibody consensus. The amino acid substitution may be, for example conservative.

Another ary embodiment of a variant antibody or antigen binding fragment include those having a light chain variable region as set forth in SEQ ID NO.:31: SEQ ID NO.:31 DXa1VMTQTPLSLXa2VX33Xa4GXa5XasASISCRSSQSLLHSNGNTYLEWYLQKPGQSP Xa7LLlHTVSNRFSGVPDRFSGSGSGTDFTLKlSRVEAEDXasGWYCFQGSHVPLTF GXaeGTXaioLEXanK, Wherein X31 may be a hydrophobic amino acid; Wherein X32 may be A or P; Wherein X33 may be neutral hilic amino acid; Wherein X34 may be L or P; n X35 may be an acidic amino acid; Wherein Xaa may be Q or P; Wherein X37 may be a basic amino acid; Wherein X38 may be a hydrophobic amino acid; Wherein Xag may be A or Q; Wherein X310 may be a basic amino acid; or Wherein X311 may be a hydrophobic amino acid, wherein at least one of the amino acid identified by X is an amino acid substitution (conservative or non-conservative) in comparison with a ponding amino acid in the polypeptide set forth in SEQ ID NO.:4.

An additional exemplary embodiment of a variant antibody or antigen binding fragment include those having a light chain le region as set forth in SEQ ID NO.:32: SEQ ID NO.:32 DXA1VMTQTPLSLXAZVXA3XA4GXA5XA5ASISCRSSQSLLHSNGNTYLEWYLQKPGQSP XA7LLIHTVSNRFSGVPDRFSGSGSGTDFTLKlSRVEAEDXAaGWYCFQGSHVPLTF GXAQGTXMOLEXAHK Wherein XA1 may be V or | Wherein XAZ may be A or P Wherein XA3 may be S or T Wherein XA4 may be L or P Wherein XA5 may be D or E Wherein XAe may be Q or P n XA7 may be K or Q Wherein XAB may be L or V Wherein XAg may be A or Q Wherein Xmo may be R or K or Wherein XA11 may be L or I, wherein at least one of the amino acid identified by X is an amino acid substitution (conservative or non-conservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ ID NO.:4. in accordance with an embodiment, the light chain variable domain variant may have a sequence as set forth in SEQ lD NO.:33 or 34: SEQ ID NO.:33 TPLSLPVTPGEPASISCRSSQSLLHSNGNTYLEWYLQKPGQSPQLLlYTVS NRFSGVPDRFSGSGSGTDFTLKISRVEAEDVGWYCFQGSHVPLTFGQGTKLEIK.

SEQ ID NO.:34 DVVMTQTPLSLPVTPGEPASISCRSSQSLLHSNGNTYLEWYLQKPGQSPKLLIYTVS NRFSGVPDRFSGSGSGTDFTLKISRVEAEDVGWYCFQGSHVPLTFGQGTKLElK.

Exemplary ments of variant antibody or antigen binding fragments include those having a heavy chain variable region as set forth in SEQ ID NO.:35.

SEQ ID NO.:35 QXQLVQSGXEXXKPGASVKXSCKASGYTFTDDYMSWVXQXXGXXLEWXGDINPY NGDTNYNQKFKGXXXXTXDXSXSTAYMXLXSLXSEDXAWYCARDPGAMDYWGQ GTXVTVSS, wherein at least one of the amino acid identified by X is an amino acid substitution (conservative or nservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ lD N02. The amino acid substitution may be, for example, an amino acid found at a corresponding position of a l human antibody or a human antibody consensus. The amino acid substitution may be, for example conservative.

Another exemplary embodiment of a variant antibody or antigen binding fragment include those having a heavy chain variable region as set forth in SEQ ID NO.:36: SEQ ID NO.:36 QXmQLVQSGngEngxmKPGASVKXb5$CKASGYTFTDDYMSWVszQXwngGngXM oLEWXm1GDINPYNGDTNYNQKFKGXMZXM3Xb14Xb15TXb15DXb1ysXmSTAYMXmgLsz OSLXb21SEDszzAWYCARDPGAMDYWGQGTXb23VTVSS, Wherein Xb1 may be a hydrophobic amino acid; Wherein sz may be P or A; Wherein Xb3 may be a hydrophobic amino acid; n Xb4 may be V or K; Wherein sz may be a hydrophobic amino acid; Wherein sz may be a basic amino acid; Wherein be may be S or A; Wherein Xba may be H or P; Wherein ng may be a basic amino acid; Wherein Xbm may be S or G; n XW may be a hydrophobic amino acid; Wherein Xb12 may be a basic amino acid; Wherein Xm may be a hydrophobic amino acid; n Xb14 may be I or T; n Xb15 may be a hydrophobic amino acid; Wherein Xms may be a hydrophobic amino acid; Wherein an may be K or T; Wherein Xm may be a neutral hydrophiiic amino acid; Wherein Xb19 may be Q or E; Wherein szo may be N or S; Wherein Xb21 may be T or R; Wherein szz may be a neutral hydrophiiic amino acid; or Wherein Xb23 may be S or L, wherein at least one of the amino acid identified by X is an amino acid tution (conservative or non-conservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ ID NO.:2.

An additional exemplary embodiment of a variant antibody or antigen binding fragment include those having a heavy chain variable region as set forth in SEQ ID NO.:37: SEQ ID NO.:37 QXB1QLVQSGXBzEX33XB4KPGASVKXBssCKASGYTFTDDYMSWVXBGQXmXBaGXBgX B10LEWXB11GDlNPYNGDTNYNQKFKGXB12X313XB14XB15TXB1BDXB17SXB188TAYMXB19 LXBZOSLXBmSEDXBZZAVYYCARDPGAMDYWGQGTX523VTVSS Wherein X31 may be I or V; Wherein X32 may be P or A; Wherein X33 may be M or V; Wherein X34 may be V or K; Wherein X35 may be M or V; Wherein X36 may be K or R; Wherein X37 may be S or A; Wherein X38 may be H or P; Wherein X39 may be K or Q; Wherein X510 may be S or G; Wherein X311 may be I or M; Wherein X312 may be K or R; Wherein X313 may be A or V; n X314 may be I or T; Wherein X315 may be L or I; Wherein X316 may be V or A; n X317 may be K or T; Wherein X315 may be S or T; Wherein X319 may be Q or E; n X320 may be N or S; Wherein X321 may be T or R; Wherein X522 may be S or T; or Wherein X323 is S or L, wherein at least one of the amino acid identified by X is an amino acid substitution (conservative or non-conservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ ID NO.:2.

In accordance with an embodiment, the heavy chain variable domain variant may have a sequence as set forth in any one of SEQ ID No.38 to 41: SEQ ID NO.:38 QVQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWMGD| N PY NGDTNYNQKFKGRVTITADTSTSTAYMELSSLRSEDTAWYCARDPGAMDYWGQG TLVTVSS.

SEQ ID NO.:39 QIQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWMGDINPY NGDTNYNQKFKGRVTITADKSTSTAYMELSSLRSEDTAWYCARDPGAMDYWGQG TLVTVSS.

SEQ ID NO.:40 QIQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWIGDINPYN GDTNYNQKFKGRATLTVDKSTSTAYMELSSLRSEDTAVYYCARDPGAMDYWGQG TLVTVSS.

SEQ ID NO. :41 QIQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVKQAPGQGLEWIGDINPYN GDTNYNQKFKGKATLTVDKSTSTAYMELSSLRSEDTAWYCARDPGAMDYWGQGT LVTVSS.

Production of the antibodies in cells The AAG1 antibodies that are disclosed herein can be made by a variety of methods familiar to those skilled in the art, such as hybridoma methodology or by recombinant DNA methods.

In an exemplary embodiment of the invention, an anti—KAAG1 antibodies (e.g., an antibody which can compete with the antibodies disclosed herewith) may be produced by the conventional hybridoma technology, where a mouse is immunized with an antigen, spleen cells ed and fused with myeloma cells lacking HGPRT expression and hybrid cells ed by hypoxanthine, aminopterin and thymine (HAT) containing media.

In an additional exemplary embodiment of the invention, the anti-KAAG1 dies may be produced by inant DNA methods. in order to express the anti-KAAG1 dies, nucleotide sequences able to encode any one of a light and heavy immunoglobulin chains described herein or any other may be inserted into an expression vector, Le, a vector that contains the elements for transcriptional and ational control of the ed coding sequence in a particular host. These elements may include regulatory ces, such as enhancers, constitutive and inducible promoters, and 5' and 3' un-translated regions. Methods that are well known to those skilled in the art may be used to construct such expression vectors. These methods include in vitro recombinant DNA techniques, synthetic ques, and in vivo genetic recombination.

A y of expression vector/host cell systems known to those of skill in the art may be utilized to express a polypeptide or RNA derived from nucleotide sequences able to encode any one of a light and heavy immunoglobulin chains described .

These include, but are not limited to, microorganisms such as bacteria transformed with recombinant bacteriophage, plasmid, or cosmid DNA expression s; yeast transformed with yeast expression vectors; insect cell systems infected with baculovirus vectors; plant cell systems transformed with viral or bacterial sion s; or animal cell systems. For long-term production of recombinant proteins in mammalian systems, stable expression in cell lines may be effected. For example, nucleotide sequences able to encode any one of a light and heavy immunoglobulin chains described herein may be transformed into cell lines using expression vectors that may contain viral origins of replication and/or endogenous expression ts and a selectable or visible marker gene on the same or on a separate vector. The invention is not to be limited by the vector or host cell employed. In n embodiments of the present invention, the nucleotide sequences able to encode one of a light and heavy immunoglobulin chains described herein may each be ligated into a separate expression vector and each chain expressed separately. In another embodiment, both the light and heavy chains able to encode any one of a light and heavy immunoglobulin chains described herein may be ligated into a single expression vector and sed simultaneously.

Alternatively, RNA and/or polypeptide may be expressed from a vector comprising nucleotide sequences able to encode any one of a light and heavy immunoglobulin chains described herein using an in vitro transcription system or a coupled in vitro transcription/translation system respectively. of a in general, host cells that contain nucleotide sequences able to encode any one light and heavy immunoglobulin chains described herein and/or that express a polypeptide encoded by the nucleotide ces able to encode any one of a light and heavy immunoglobulin chains described herein, or a portion thereof, may be identified by a variety of procedures known to those of skill in the art. These procedures include, but are not limited to, DNA/DNA or DNA/RNA hybridizations, PCR amplification, and protein bioassay or immunoassay techniques that include membrane, solution, or chip based technologies for the detection and/or quantification of nucleic acid or amino acid sequences. Immunological methods for detecting and measuring the expression of polypeptides using either specific polyclonal or monoclonal antibodies are known in the art. Examples of such techniques include enzyme-linked immunosorbent assays (ELISAs), radioimmunoassays (RlAs), and fluorescence ted cell sorting (FACS). Those of skill in the art may readily adapt these methodologies to the present invention.

Host cells comprising nucleotide sequences able to encode any one of a light and heavy immunoglobulin chains described herein may thus be cultured under conditions for the transcription of the corresponding RNA (mRNA, etc.) and/or the expression of the polypeptide from cell e. The polypeptide produced by a cell may be secreted or may be ed intracellularly depending on the ce and/or the vector used.

In an exemplary embodiment, expression s ning tide sequences able to encode any one of a light and heavy immunoglobulin chains described herein secretion of the polypeptide may be designed to contain signal ces that direct through a prokaryotic or otic cell membrane.

Due to the inherent degeneracy of the genetic code, other DNA sequences that encode the same, substantially the same or a onally equivalent amino acid sequence may be produced and used, for e, to express a polypeptide encoded by nucleotide sequences able to encode any one of a light and heavy immunoglobulin chains described herein. The nucleotide ces of the present invention may be engineered using methods generally known in the art in order to alter the tide sequences for a variety of purposes including, but not limited to, modification of the cloning, processing, and/or expression of the gene product. DNA shuffling by random fragmentation and PCR reassembly of gene fragments and synthetic oligonucleotides may be used to engineer the nucleotide ces. For example, oligonucleotide-mediated site-directed mutagenesis may be used to introduce mutations that create new restriction sites, alter glycosylation patterns, change codon preference, produce splice variants, and so forth.

In addition, a host cell strain may be chosen for its ability to te expression of the inserted ces or to process the expressed polypeptide in the desired fashion. Such modifications of the polypeptide include, but are not d to, acetylation, carboxylation, glycosylation, phosphorylation, lipidation, and acylation. In an exemplary embodiment, anti-KAAG1 antibodies that contain particular glycosylation ures or patterns may be desired. Post-translational processing, which cleaves a “prepro” form of the polypeptide, may also be used to specify protein targeting, folding, and/or activity. Different host cells that have specific cellular machinery and characteristic mechanisms for ranslational activities (e.g., CHO, HeLa, MDCK, HEK293, and W138) are available commercially and from the American Type e Collection (ATCC) and may be chosen to ensure the correct modification and processing of the expressed polypeptide.

Those of skill in the art wi|l readily appreciate that natural, modified, or recombinant nucleic acid sequences may be ligated to a heterologous sequence resulting in translation of a fusion polypeptide containing heterologous polypeptide moieties in any of the entioned host s. Such heterologous polypeptide moieties may facilitate purification of fusion polypeptides using commercially available affinity es. Such moieties include, but are not limited to, glutathione S-transferase (GST), e g protein, thioredoxin, calmodulin binding peptide, 6-His (His), FLAG, c-myc, utinin (HA), and antibody epitopes such as monoclonal antibody epitopes.

In yet a further aspect, the present invention relates to a cleotide which may comprise a nucleotide sequence encoding a fusion protein. The fusion protein may comprise a fusion partner (e.g., HA, Fc, etc.) fused to the polypeptide (e.g., complete light chain, complete heavy chain, variable regions, CDRs etc.) described .

Those of skill in the art will also y recognize that the nucleic acid and polypeptide sequences may be synthesized, in whole or in part, using chemical or enzymatic methods well known in the art. For example, peptide synthesis may be performed using various solid-phase techniques and machines such as the ABI 431A Peptide synthesizer (PE Biosystems) may be used to automate synthesis. If desired, the amino acid sequence may be altered during sis and/or combined with sequences from other proteins to produce a variant protein.

Antibody coniugates The antibody or antigen binding fragment of the t invention may be conjugated with a detectable moiety (i.e., for ion or diagnostic purposes) or with a therapeutic moiety (for therapeutic purposes).

A table moiety" is a moiety detectable by spectroscopic, photochemical, mical, immunochemical, al and/or other physical means. A detectable moiety may be d either directly and/or indirectly (for example via a linkage, such as, without limitation, a DOTA or NHS linkage) to antibodies and antigen binding nts thereof of the present invention using methods well known in the art. A wide variety of detectable moieties may be used, with the choice depending on the sensitivity required, ease of conjugation, stability requirements and available instrumentation. A suitable detectable moiety include, but is not limited to, a fluorescent label, a radioactive label (for example, t limitation, 125l, Inm, T099, l131 and including positron emitting isotopes for PET scanner etc), a nuclear magnetic resonance active label, a luminiscent label, a uminescent label, a chromophore label, an enzyme label (for example and without limitation horseradish peroxidase, alkaline phosphatase, etc.), quantum dots and/or a nanoparticle. Detectable moiety may cause and/or produce a detectable signal thereby ng for a signal from the detectable moiety to be detected.

In r ary embodiment of the invention, the antibody or antigen binding nt thereof may be coupled (modified) with a therapeutic moiety (e.g., drug, cytotoxic moiety, anti-cancer agent).

In an exemplary embodiment, the anti-KAAG1 antibodies and antigen binding fragments may comprise a chemotherapeutic, a cytotoxic agent or an anti-cancer drug (e.g., small molecule). Such chemotherapeutic or cytotoxic agents include, but are not limited to, Yttrium-90, Scandium-47, m-186, Iodine-131, Iodine-125, and many others recognized by those skilled in the art (e.g., lutetium (e.g., Lum), bismuth (e.g., Bi213), copper (e.g., Cu67)). In other instances, the chemotherapeutic, cytotoxic agent or anti-cancer drug may be comprised of, among others known to those skilled in the art, 5-fluorouracil, adriamycin, irinotecan, taxanes, pseudomonas endotoxin, ricin, auristatins (e.g., monomethyl auristatin E, monomethyl auristatin F), maytansinoids (e.g., mertansine) and other toxins.

Alternatively, in order to carry out the methods of the present invention and as known in the art, the antibody or antigen binding fragment of the present invention (conjugated or not) may be used in ation with a second molecule (e.g., a secondary antibody, etc.) which is able to specifically bind to the antibody or antigen binding fragment of the present invention and which may carry a desirable detectable, diagnostic or therapeutic .

Pharmaceutical compositions of the antibodies and their use Pharmaceutical compositions of the anti-KAAG1 antibodies or antigen binding fragments (conjugated or not) are also encompassed by the present invention. The pharmaceutical ition may comprise an anti-KAAG1 antibody or an antigen binding nt and may also contain a pharmaceutically acceptable carrier.

Other s of the invention relate to a ition which may comprise the antibody or antigen binding fragment described herein and a carrier.

The present invention also relates to a pharmaceutical composition which may comprise the antibody or antigen g fragment described herein and a pharmaceutically acceptable carrier.

In addition to the active ingredients, a pharmaceutical composition may contain pharmaceutically able carriers comprising water, PBS, salt solutions, gelatins, oils, alcohols, and other excipients and auxiliaries that facilitate processing of the active compounds into preparations that may be used pharmaceutically. In other ces, such preparations may be sterilized.

As used herein, "pharmaceutical composition" means therapeutically effective amounts of the agent er with pharmaceutically able diluents, preservatives, solubilizers, emulsifiers, adjuvant and/or carriers. A "therapeutically ive amount" as used herein refers to that amount which es a therapeutic effect for a given condition and administration n. Such compositions are liquids or lized or otherwise dried formulations and include diluents of various buffer content (e.g., Tris-HCI., acetate, phosphate), pH and ionic strength, additives such albumin or gelatin to prevent absorption to surfaces, detergents (e.g., Tween 20, Tween 80, Pluronic F68, bile acid . Solubilizing agents (e.g., glycerol, hylene glycerol), anti-oxidants (e.g., ascorbic acid, sodium metabisulfite), preservatives (e.g., thimerosal, benzyl alcohol, parabens), bulking substances or tonicity modifiers (e.g., lactose, mannitol), covalent attachment of polymers such as polyethylene glycol to the n, xation with metal ions, or incorporation of the material into or onto particulate preparations of ric compounds such as ctic acid, polyglycolic acid, hydrogels, etc, or onto liposomes, microemulsions, micelles, unilamellar or multilamellar vesicles, erythrocyte ghosts, or spheroplasts.

Such compositions will influence the physical state, solubility, stability, rate of in vivo release, and rate of in vivo clearance. Controlled or sustained release compositions include formulation in lipophilic depots (e.g., fatty acids, waxes, oils). Also comprehended by the invention are particulate compositions coated with polymers (e.g., poloxamers or poloxamines). Other embodiments of the compositions of the invention incorporate particulate forms protective gs, protease inhibitors or tion enhancers for various routes of administration, including parenteral, pulmonary, nasal, oral, vaginal, rectal routes. In one embodiment the pharmaceutical composition is administered parenterally, paracancerally, transmucosally, transdermally, intramuscularly, intravenously, intradermally, subcutaneously, intraperitonealy, intraventricularly, intracranially and intratumorally.

Further, as used herein "pharmaceutically acceptable carrier" or "pharmaceutical carrier" are known in the art and include, but are not limited to, 0.01-O.1 M or 0.05 M phosphate buffer or 0.8 % saline. Additionally, such pharmaceutically acceptable carriers may be aqueous or non—aqueous solutions, suspensions, and emulsions.

Examples of ueous solvents are propylene , polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl .

Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, ing saline and buffered media. Parenteral es include sodium chloride on, Ringer's dextrose, dextrose and sodium de, lactated Ringer's d oils. intravenous es include fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose, and the like. Preservatives and other additives may also be present, such as, for example, antimicrobials, antioxidants, collating agents, inert gases and the like.

For any compound, the therapeutically effective dose may be estimated initially either in cell culture assays or in animal models such as mice, rats, rabbits, dogs, or pigs.

An animal model may also be used to determine the concentration range and route of administration. Such information may then be used to determine useful doses and routes for administration in humans. These techniques are well known to one skilled in the art and a therapeutically effective dose refers to that amount of active ingredient that ameliorates the symptoms or condition. Therapeutic cy and toxicity may be determined by standard pharmaceutical procedures in cell cultures or with experimental animals, such as by calculating and contrasting the E050 (the dose therapeutically effective in 50% of the population) and LD5o (the dose lethal to 50% of the population) statistics. Any of the eutic compositions described above may be applied to any subject in need of such therapy, including, but not limited to, mammals such as dogs, cats, cows, horses, rabbits, monkeys, and humans.

The pharmaceutical compositions utilized in this invention may be stered by any number of routes including, but not limited to, oral, intravenous, uscular, intra—arterial, edullary, intrathecal, entricular, transdermal, aneous, intraperitoneal, intranasal, l, topical, sublingual, or rectal means.

The term "treatment" for purposes of this disclosure refers to both therapeutic treatment and prophylactic or preventative measures, wherein the object is slow down (lessen) the targeted pathologic condition or disorder. Those in need of treatment include those already with the disorder as well as those prone to have the disorder or those in whom the disorder is to be prevented. Particularly, subjects in need include subjects with an elevated level of one or more cancer markers.

The anti-KAAG1 antibodies and antigen g fragments thereof may have eutic uses in the treatment of various cancer types, such as ovarian cancer, renal cancer, colon cancer, lung cancer, melanoma, etc. In an ary embodiment, the antibodies and fragments have therapeutic uses in ovarian cancer.

In a more particular embodiment the subject may have, for example, a recurrent ovarian cancer. in yet another embodiment, the subject may have, for example, a metastatic cancer. in certain ces, the AAG1 antibodies and fragments may block the interaction of KAAG1 with its protein partners. The anti-KAAG1 antibodies of the present invention may particularly be used to deliver a eutic moiety to a cell expressing KAAGt.

The anti-KAAGt dies and antigen binding fragments thereof may have therapeutic uses in the treatment of various types of ovarian cancer. Several different cell types may give rise to ent ovarian cancer histotypes. The most common form of ovarian cancer is comprised of tumors that originate in the epithelial cell layer of the ovary or the fallopian tube. Such epithelial ovarian cancers include serous tumors, endometroid tumors, mucinous tumors, clear cell tumors, and borderline tumors. In other embodiments, the anti-KAAG1 antibodies and antigen binding fragments thereof have uses in the ent of other types of ovarian cancer such as germ line and sex cord ovarian cancer.

In n instances, the AAG1 antibodies and n binding nts thereof may be administered concurrently in combination with other treatments given for the same condition. As such, the antibodies may be administered with anti-mitotics (eg., taxanes), platinum-based agents (eg., cisplatin), DNA damaging agents (eg.

Doxorubicin) and other anti-cancer therapies that are known to those skilled in the art.

In other instances, the anti-KAAG1 antibodies and antigen binding fragments thereof may be administered with other therapeutic antibodies. These include, but are not limited to, antibodies that target EGFR, CD—20, and Her2.

The present invention relates in a further aspect thereof to a method for inhibiting the growth of a KAAG1-expressing cell, the method which may comprise contacting the cell with an effective amount of the antibody or antigen binding fragment described herein.

The present invention also asses method of ng cancer or inhibiting the growth of a KAAG1 expressing cells in a mammal, the method may comprise administering the antibody or antigen binding fragment, for example, conjugated with a eutic moiety described herein to a subject in need.

In further aspects, the present invention provides method of treatment, stic methods and method of detection using the antibody or antigen binding fragment of the present invention and the use of these dies or antigen binding fragment in the manufacture of a pharmaceutical composition or drug for such es.

The invention therefore relates to the use of the ed antibody or antigen binding fragment described herein in the (manufacture of a pharmaceutical ition for) treatment of cancer.

The antibody or antigen g fragment may more particularly be applicable for malignant tumors including, for example, a malignant tumor having the ability to metastasize and/or tumor cells characterized by age-independent growth.

The antibody or antigen binding fragment of the present invention may also be used in the diagnosis of cancer. The sis of cancer may be performed in vivo by administering the antibody or antigen binding fragment of the present invention to a mammal having or suspected of having a cancer. The diagnosis may also be performed ex vivo by contacting a sample obtained from the mammal with the antibody or antigen binding fragment and determining the presence or absence of cells (tumor cells) expressing KAAGl or a KAAG1 variant.

The present invention ore also encompasses method of detecting cancer or ing a KAAGl expressing cells in a , the method may comprise administering the antibody or antigen binding nt described herein to a subject in need.

The present invention relates in another aspect thereof to a method for detecting a cell sing KAAG1 or a KAAG1 variant, the method may comprise contacting the cell with an antibody or antigen g fragment described herein and detecting a complex formed by the antibody and the or KAAG1 variant-expressing cell.

Exemplary embodiments of antibodies or antigen binding fragments used in detection methods are those which are capable of binding to the ellular region of KAAG1.

Other exemplary embodiments of antibodies or antigen binding fragments used in detection methods are those which bind to KAAG1 or KAAG1 variant expressed at the surface of a tumor cells.

Subject in need which would benefit from treatment, ion or diagnostic methods described herein are those which have or are suspected of having cancer, e.g., ovarian cancer (e.g., serous, endometroid, clear cell or mucinous), skin cancer (e.g., mas, squamous cell carcinomas), renal cancer (e.g., papillary cell carcinomas, clear cell carcinomas), colorectal cancer (e.g., colorectal carcinomas), sarcoma, leukemia, brain tumor, thyroid tumor, breast cancer (e.g., mammary carcinomas), prostate cancer (e.g., prostatic carcinomas), oesophageal tumor, bladder tumor, lung tumor (e.g., lung carcinomas) or head and neck tumor and especially when the cancer is characterized as being malignant and/or when the cells sing KAAG1 or a KAAG1 variant are characterized by anchorage-independent growth.

Subjects having cancer may be identified by imaging, tissue biopsy, genetic testing.

Alternatively, subjects having cancer may be identified by the presence of cancer markers in their bodily fluids using standard assays (e.g., ELISA and the like).

Especially encompassed by the present invention are patients having or susceptible of having ovarian cancer (e.g., serous, troid, clear cell or mucinous), skin cancer (e.g., melanomas, squamous cell carcinomas) or renal cancer (e.g., papillary cell carcinomas) and especially when the cancer is characterized as being malignant and/or when the cells expressing KAAG1 or a KAAG1 variant are characterized by anchorage-independent growth.

Another aspect of the invention s to a method for detecting KAAG1 (SEQ ID NO.:29), a KAAG1 variant having at least 80% sequence identity with SEQ ID NO.:29 or a secreted form of circulating form of KAAG1 or KAAG1 variant, the method may comprise ting a cell expressing KAAG1 or the KAAG1 variant or a sample (biopsy, serum, plasma, urine etc.) comprising or suspected of comprising KAAG1 or the KAAG1 variant with the dy or antigen binding fragments described herein and measuring binding. The sample may originate from a mammal (e.g., a human) which may have cancer (e.g., ovarian , a atic cancer) or may be suspected of having cancer (e.g., ovarian , a metastatic cancer). The sample may be a tissue sample obtained from the mammal or a cell culture supernatant. in accordance with the invention the sample may be a serum sample, a plasma sample, a blood sample, semen or ascitic fluid obtained from the mammal. The antibody or antigen binding fragment described herein may advantageously detect a secreted or circulating form (circulating in blood) of KAAG1.

The method may comprise fying the complex formed by the antibody or n binding fragment bound to KAAG1 or to the KAAG1 variant.

The binding of an antibody to an antigen will cause an increase in the expected molecular weight of the n. A physical change therefore occurs upon specific binding of the dy or antigen binding fragment and the antigen.

Such changes may be detected using, for example, electrophoresis followed by Western blot and coloration of the gel or blot, mass spectrometry, HPLC coupled with a computer, FACS or else. Apparatus capable of computing a shift in molecular weight are known in the art and include for example, PhosphorimagerTM.

When the antibody comprises for example a detectable label, the antigen-antibody complex may be ed by the fluorescence emitted by the label, radiation emission of the label, enzymatic activity of a label provided with its substrate or else. ion and/or measurement of binding between an antibody or antigen binding nt and an antigen may be performed by s methods known in the art.

Binding between an antibody or antigen binding fragment and an antigen may be monitored with an apparatus capable of ing the signal emitted by the detectable label (radiation emission, fluorescence, color change etc.). Such apparatus provides data which indicates that binding as ed and may also provide indication as to the amount of antibody bound to the n. The apparatus (usually coupled with a computer) may also be capable of calculating the difference between a background signal (e.g., signal ed in the absence of antigen-antibody binding) or ound noise and the signal obtained upon specific antibody—antigen binding.

Such apparatuses may thus provide the user with indications and conclusions as to whether the antigen has been detected or not.

Additional aspects of the invention relates to kits which may include one or more ner containing one or more antibodies or antigen binding fragments bed herein.

Nucleic acids, vectors and cells Antibodies are usually made in cells allowing expression of the light chain and heavy chain expressed from a vector(s) comprising a nucleic acid ce encoding the light chain and/or heavy chain.

The present therefore encompasses nucleic acids capable of encoding any of the CDRs, light chain variable regions, heavy chain variable regions, light chains, heavy chains described herein.

The present invention therefore relates in a further aspect to a c acid encoding a light chain variable region and/or a heavy chain variable region of an antibody which is e of ic binding to KAAG1.

Exemplary embodiments of nucleic acids of the present invention include nucleic acids encoding a light chain variable region comprising: a. a CDRL1 as set forth in SEQ ID NO.:8 or comprising SEQ ID NO.:8; b. a CDRL2 as set forth in SEQ ID NO.:9 or comprising SEQ ID NO.:9, c. a CDRL3 sequence as set forth in SEQ ID NO.:10 or comprising SEQ ID NO.:10.

In accordance with the present invention, the nucleic acid may encode a light chain variable region which may se at least two CDRs of a CDRL1, a CDRL2 or a CDRL3.

Also in accordance with the present invention, the nucleic acid may encode a light chain le region which may comprise one CDRL1, one CDRL2 and one CDRL3.

The present invention also relates to a nucleic acid encoding a heavy chain variable region comprising: a. a CDRH1 sequence as set forth in SEQ ID NO.:5 or comprising SEQ ID NO.:5; b. a CDRH2 sequence as set forth in SEQ ID NO.:6 or comprising SEQ ID NO.:6, or; c. a CDRH3 sequence as set forth in SEQ ID NO.:7 or comprising SEQ ID NO.:7.

In accordance with the present invention, the nucleic acid may encode a heavy chain le region which may comprise at least two CDRs of a CDRH1, a CDRH2 or a CDRH3.

In accordance with the present invention, the nucleic acid may encode a heavy chain le region which may comprise one CDRH1, one CDRH2 and one CDRH3.

Also encompassed by the present invention are nucleic acids encoding antibody variants having at least one conservative amino acid substitution.

In accordance with the present invention, the nucleic acid may encode a CDR comprising at least one conservative amino acid substitution.

In accordance with the present invention, the nucleic acid may encode a CDR comprising at least one conservative amino acid substitution in at least two of the CDRs.

In accordance with the present invention, the nucleic acid may encode a CDR comprising at least one conservative amino acid substitution in the 3 CDRs.

In accordance with the present invention, the nucleic acid may encode a CDR comprising at least two conservative amino acid substitutions in at least one of the CDRs.

In accordance with the present invention, the nucleic acid may encode a CDR comprising at least two conservative amino acid substitutions in at least two of the CDRs.

In accordance with the present invention, the nucleic acid may encode a CDR comprising at least two conservative amino acid substitutions in the 3 CDRs.

Other aspects of the invention relate to a nucleic acid encoding a light chain le region having at least 70%. 75%, 80% sequence identity to SEQ ID NO.:4.

Yet other aspects of the invention relate to a nucleic acid encoding a heavy chain variable region having at least 70%. 75%, 80% sequence identity to SEQ ID NO.:2.

In yet another aspect, the present ion relates to a vector comprising the nucleic acids bed .

In accordance with the present invention, the vector may be an expression .

Vector that ns the elements for riptional and translational control of the inserted coding sequence in a particular host are known in the art. These elements may include regulatory sequences, such as enhancers, tutive and inducible promoters, and 5' and 3' un-translated regions. Methods that are well known to those skilled in the art may be used to construct such expression vectors. These methods include in vitro inant DNA techniques, synthetic techniques, and in vivo genetic recombination.

In r aspect the present invention relates to an isolated cell which may comprise the nucleic acid, dies or antigen binding fragment described herein.

The ed cell may comprise a nucleic acid encoding a light chain le region and a nucleic acid encoding a heavy chain variable region either on separate vectors or on the same . The isolated cell may also comprise a nucleic acid encoding a light chain and a nucleic acid encoding a heavy chain either on separate vectors or on the same vector.

In accordance with the present invention, the cell may be capable of expressing, assembling and/or secreting an dy or antigen g fragment thereof.

In another aspect, the present invention provides a cell which may comprise and/or may express the antibody described herein.

In accordance with the invention, the cell may comprise a nucleic acid ng a light chain variable region and a nucleic acid encoding a heavy chain variable region.

The cell may be capable of expressing, assembling and/or secreting an antibody or antigen binding fragment thereof.

The examples below are presented to further outline details of the present invention.

EXAMPLES Example 1 This example describes the binding of antibody 3A4 to KAAG1.

The antibodies that bind KAAG1 were generated using the Alere phage y technology. A detailed ption of the technology and the methods for generating these antibodies can be found in the US. Patent No. 6,057,098. In addition, a detailed description of the generation of antibodies against KAAG1 can be found in 2009/001586. Briefly, the technology es stringent panning of phage libraries that display the antigen binding nts (Fabs). After a several rounds of panning, a library, termed the Omniclonal, was obtained that was enriched for recombinant Fabs containing light and heavy chain variable regions that bound to KAAGI with very high affinity and specificity. From this library, more precisely designated Omniclonal AL0003 AZZB, 96 individual recombinant monoclonal Fabs were prepared from E. coli and tested for KAAG1 binding. The monoclonal designated 3A4 was derived from this 96-well plate of onal antibodies based on its high binding activity for recombinant KAAG1 and its affinity for KAAG1 on the surface of ovarian cancer cells.

The nucleotide sequences of the variable regions of the heavy and light chain immunoglobulin chains are shown in SEQ ID NOS.:1 and 3, respectively and the polypeptide sequences of the variable regions of the heavy and light chain immunoglobulin chains are shown in SEQ ID NOS.:2 and 4, tively. The complementarity determining regions (CDRs) of the 3A4 heavy chain immunoglobulin are shown in SEQ ID NOS.:5, 6 and 7, respectively and the CDRs of the 3A4 light chain immunoglobulin are shown in SEQ ID NOS.:8, 9 and 10, respectively.

Aside from the possibility of conducting interaction s between the Fab monoclonals and the KAAG1 protein, the use of Fabs is limited with t to conducting meaningful in vitro and in vivo studies to te the biological on of the antigen. Thus, it was necessary to transfer the light and heavy chain variable regions contained in the 3A4 Fabs to full dy scaffolds, to generate mouse— human chimeric IgG1. The expression vectors for both the light and heavy immunoglobulin chains were constructed such that i) the original bacterial signal peptide sequences upstream of the Fab expression vectors were replaced by mammalian signal peptides and ii) the light and heavy chain constant regions in the mouse antibodies were replaced with human constant regions. The methods to accomplish this er utilized standard molecular biology techniques that are familiar to those skilled in the art. A brief overview of the methodology is described here.

Light chain expression vector — an existing mammalian expression pIasmid, called pTTVHBG (Durocher et al., 2002), ed to be used in the 293E transient transfection system was modified to accommodate the mouse light chain variable region. The resuIting mouse-human chimeric light chain contained a mouse variable region followed by the human kappa constant . The cDNA sequence encoding the human kappa constant domain was amplified by PCR with s OGS1773 and OGS1774 (SEQ ID NOS:11 and 12, respectively). The nucleotide sequence and the ponding amino acid sequence for the human kappa constant region are shown in SEQ ID NOS:13 and 14, respectively. The resulting 321 base pair PCR product was ligated into pTTVH8G ately downstream of the signal peptide sequence of human VEGF A (NM_003376). This cloning step also positioned unique restriction endonuclease sites that permitted the precise positioning of the cDNAs encoding the mouse light chain variable regions. The sequence of the final expression plasmid, called pTTVK1, is shown in SEQ ID NO.:15. Based on the 3A4 light chain variable sequence shown in SEQ lD NO.:3, a PCR primer specific for the light chain variable region was designed that incorporated, at its 5’-end, a sequence cal to the last base pairs of the VEGF A signal peptide. The sequence of this primer is shown in SEQ ID NO:16. A reverse primer (SEQ ID NO.:17) incorporated, at its 3’-end, a sequence identical to the first 20 base pairs of the human kappa constant domain.

Both the PCR fragments and the digested pTTVK1 were d with the 3’ — 5’ lease activity of T4 DNA polymerase resulting in complimentary ends that were joined by annealing. The annealing reactions were transformed into ent E. coli and the expression plasmids were verified by sequencing to ensure that the mouse light chain variable regions were properly inserted into the pTTVK1 expression vector.

Heavy chain expression vector— the expression vector that produced the 3A4 heavy chain immunoglobulin was designed in a similar manner to the pTTVK1 described above for production of the light chain immunoglobulins. Plasmid pYD11 her et al., 2002), which contains the human lgGK signal peptide sequence as well as the CH2 and CH3 s of the human Fc domain of lgG1, was modified by ligating the cDNA sequence encoding the human constant CH1 region. PCR primers OGS1769 and OGS1770 (SEQ ID NOS:18 and 19), designed to contain unique restriction endonuclease sites, were used to amplify the human lgG1 CH1 region containing the nucleotide sequence and corresponding amino acid sequence shown in SEQ ID NOS:2O and 21. Following ligation of the 309 base pair fragment of human CH1 ately downstream of the lgGK signal e sequence, the modified plasmid (SEQ ID ) was designated pYD15. When a selected heavy chain variable region is ligated into this vector, the ing plasmid encodes a full IgG1 heavy chain immunoglobulin with human constant regions. A PCR primers ic for the heavy chain variable region of antibody 3A4 (SEQ ID NOS:1) was designed that incorporated, at its 5’-end, a sequence identical to the last 20 base pairs of the lgGK signal peptide. The sequence of this primers is shown in SEQ ID NOS:23. A reverse primer (SEQ lD ) incorporated, at its 3’-end, a sequence identical to the first 20 base pairs of the human CH1 constant . Both the PCR fragments and the digested pYDi5 were treated with the 3’ — 5’ exonuclease activity of T4 DNA polymerase resulting in complimentary ends that were joined by annealing. The annealing reactions were transformed into competent E. coli and the expression plasmids were verified by sequencing to ensure that the mouse heavy chain variable regions were properly inserted into the pYD15 expression vector.

Expression of human 3A4 chimeric [961 in 293E cells — The sion vectors prepared above that encoded the light and heavy chain globulins were sed in 293E cells using the transient transfection system (Durocher et al., 2002). The ratio of light to heavy chain was zed in order to achieve the most yield of antibody in the tissue culture medium and it was found to be 9:1 (L2H).

Binding of chimeric 3A4 to KAAG1 — To measure the ve binding of the 3A4 monoclonal antibody, recombinant human KAAG1 was produced in 293E cells using the large-scale transient transfection technology (Durocher et al., 2002; Durocher, 2004). The expression and purification of human recombinant KAAG1 as an F0 fusion protein is found in . To carry out the binding of Fc-KAAG1 to the antibody preparation, the Fc—KAAG1 was biotinylated with NHS-biotin (Pierce, Rockford, IL) and 10 ng/well was coated in a streptavidin 96-well plate for 1h at room temperature. Purified chimeric 3A4 was added at increasing concentrations and incubated at room temperature for 30 minutes. Bound dy was detected with HRP-conjugated human anti-kappa light chain antibody in the presence of TMB liquid substrate (Sigma-Aldrich Canada Ltd., Oakville, ON) and readings were conducted at 450 nm in microtiter plate reader. As shown in Figure 1, 3A4 interacted with the immobilized KAAG1 protein in a dose-dependent manner. When the control unrelated lgG was incubated with the recombinant KAAG1, no g activity was observed, even at the very highest tration. This result demonstrated that 3A4 binds to human KAAGl. The binding of 3A4 was compared to the binding of the chimeric 3D3 (described in Tremblay and Filion (2009)), that has different epitope city (see Example 2). The binding ty of 3A4 is very r to 3D3 in this type of assay (see Figure 1).

Example 2 This example describes the epitope g studies to determine which region of KAAG1 the 3A4 antibody binds to.

To further delineate the regions of KAAG1 that are bound by the 3A4 antibody, truncated mutants of KAAG1 were expressed and used in the ELISA. As for the full length KAAG1, the truncated versions were amplified by PCR and ligated into BamHI/HindIII digested pYD5. The primers that were used combined the forward ucleotide with the sequence shown in SEQ ID NO.:25 with primers of SEQ ID NOS:26 and 27, to produce Fc-fused fragments that ended at amino acid number 60 and 35 of KAAG1, respectively. The expression of these recombinant mutants was conducted as was described above for the full length Fc-KAAG1 and purified with Protein-A agarose.

Based on the teachings of Tremblay and Filion (2009), it was known that other antibodies interacted with specific regions of recombinant KAAG1. Thus, anti-KAAG1 dy 3C4, 3D3, and 3G10 interacted with the regions 1 - 35, 36 — 60, and 61 ~ 84 of KAAG1, tively. These binding results were reproduced and are shown in Figure 2. In order to determine the region in KAAG1 that is bound by the 3A4 antibody, the ELISA was performed using the KAAG1 truncated Fc-fusions according to a similar protocol that was described in Example 1. The only modifications were the use of different biotinylated Fc-KAAG1 truncated mutants. The results show that the binding specificity of 3A4 is similar to 3G10. In KAAG1 mutants that do not have amino acids sequences beyond amino acid 60, the binding of 3A4 to KAAG1 does not occur. This indicates that 3A4 interacts with a region delineated by amino acids 61 — 84 of human KAAG1. The observation that 3A4 and 3D3 have lly cal binding activity as measured by ELISA le 1) but have very different epitope specificity suggests that the binding ties of 3A4 is quite distinct of 3D3.

Example 3 This example describes the ability of 3A4 to bind to KAAG1 on the surface of cancer cell lines Flow cytometry was used to detect KAAG1 on the surface of cell lines. Based on RT- PCR expression analyses using KAAG1 mRNA specific primers, selected cancer cell lines were expected to express KAAG1 protein. To verify this, ovarian cancer cells 3 and TOV-21G) and a control cell lines that showed very little KAAG1 expression (293E). The cells were harvested using 5 mM EDTA, counted with a hemocytometer, and resuspended in FCM buffer (0.5% BSA, 10 ug/ml goat serum in 1x PBS) at a cell density of 2 x 106 cells/ml. Chimeric 3A4 or a l IgG were added to 100 pl of cells at a final concentration of 5 pig/ml and incubated on ice for 2h.

The cells were washed in cold PBS to remove unbound antibodies, resuspended in 100 pl FCM buffer containing anti—human lgG conjugated to FITC (diluted 1:200) as a secondary antibody and ted on ice for 45min on ice. Following another washing step in cold PBS, the cells were resuspended in 250 pl FCM buffer and analyzed with a flow cytometer. The results from this experiment are shown in Figure 3A and 38.

Incubation of the cell lines with the control antibody resulted in histograms that corresponded to the signal that was typically obtained when the antibody was omitted from the cells. This established the background signal of these FCM values (Figures 3A and 38). By contrast, incubation of the SKOV-3, TOV—21G with the 3A4 chimeric antibody resulted in a strong fluorescence signal (Figures 3A). This indicated that the dy ently detects KAAG1 on the e of these cancer cells. The 293E cells, a human kidney cell line, was expected to show very little KAAG1 expression and indeed, FCM histogram showed almost no shift compared to the control antibody (see Figure 38). Therefore, 3A4 specifically ed KAAG1 on the surface of cancer cells. The activity of 3A4 was compared to the 303, an AAG1 antibody described in the teachings of Tremblay and Filion (2009). Based on this patent application, it was known that 3D3 could detect KAAG1 on the surface of cancer cells as measured by FCM. This was confirmed when the 3D3 was incubated in the presence of SKOV-3 and TOV-21G cells (see Figure 3A). The fluorescence signal was not as high compared to the 3A4, indicating that 3A4 has different and increased ability to detect KAAG1 on the surface of n cancer cells. Other results obtained in our laboratory indicate that 3A4 could detect KAAG1 on the surface of cancer cells under conditions where 3D3 exhibited no activity in this assay ts not .

Taken together, these observations and the difference in epitope specificity of 3A4 compared to 3D3 suggests that these antibodies have distinct anti-KAAG1 properties.

Example 4 Methods for use of the 3A4 anti-KAAG1 antibody as an dy conjugate As demonstrated above, the KAAG1 antigen was detected by 3A4 on the surface of cancer cells using flow cytometry. There are several different molecular events that can occur upon binding of an dy to its target on the surface of cells. These include i) blocking accessibility to another cell-surface antigen/receptor or a ligand, ii) formation of a relatively stable antibody-antigen complex to allow cells to be ed via ADCC or CDC, iii) signalling events can occur as exemplified by agonistic antibodies, iv) the complex can be internalized, or v) the x can be shed from the cell surface. To address this question we wished to examine the behavior of the 3A4 antibody-KAAG1 complex on the surface of the cells. SKOV-3 cells were plated, washed, and incubated with 5 ug/ml chimeric 3A4 antibody as described in Example 3. After washing, complete OSE medium was added and the cells placed at 37 C for up to 90 minutes. The cells were removed at the indicated times (see Figure 4), rapidly cooled, prepared for cytometry with onjugated anti-human lgG and the results were expressed as the percentage of mean fluorescence ity (Mean fluorescence intensity, %) remaining. As illustrated in Figure 4, the fluorescence signal ses rapidly over a period of 30 - 45 minutes. This result indicates that the 3A4/KAAG1 complex disappeared from the cells, which indicated that an internalization of the complex likely occurred. Preliminary studies to elucidate the ism responsible for this decrease in urface fluorescence have revealed that the complex appears to be internalized.

These findings were further confirmed by conducting immunofluorescence on live cells to see if this internalization could be copically observed. SKOV-3 cells were seeded on cover slips in full medium (OSE medium (Wisent) containing 10% FBS, 2 mM glutamine, 1 mM sodium-pyruvate, 1X non-essential amino acids, and antibiotics). Once the cells were properly d, fresh medium was added containing the 3A4 anti-KAAG1 chimeric antibody at 10 ug/ml and incubating at 37 C for 4h. The cells were washed in PBS then fixed in 4% paraformaldehyde (in PBS) for 20 min. After washing, the cells were permeabilized with 0.1% Triton X-100 in PBS for 5 min. Blocking was performed with 1.5% dry milk in PBS for 1h. Lysosomal- associated membrane protein 1 , Chang et al., 2002) was detected by incubating with anti—LAMP1 (Santa Cruz, sc-18821, diluted 1:100) in 1.5 % milk in PBS for 2h. After washing in PBS, the secondary antibodies were added together in 1.5% milk and incubated for 1h. For the anti—KAAG1 chimeric antibodies the secondary antibody was a ine Red-X conjugated donkey uman lgG (H+L) diluted 1:300. For the anti-LAMP1 antibody the secondary antibody was a DyLight488-conjugated goat anti-mouse lgG (H+L) diluted 1:300. Both secondary antibodies were from Jackson ImmunoResearch. The coverslips were washed in PBS and mounted in ProLong Gold antifade t with DAPl. As seen in Figure 5A, after 4 hours of incubation at 37 C in the presence of SKOV-3 ovarian cancer cells, the 3A4 antibody was able to be ed in complexes predominantly near the peri-nuclear area (arrows, see red staining in the left panel in Figure 5A), which is typical of endosomal-Iysosomal-based internalization pathways. This observation was further confirmed when a mal marker, LAMP1 was visualized and was found to be also expressed in these areas s, see green staining in the middle panel in Figure 5A). Importantly, the merging of the two images resulted in the 3A4 and the anti-LAMP1 appearance of yellow-orange structures indicating that the antibodies were present in the same structures s, see yellow ng in the right panel in Figure 5A). The co-localization of 3A4, which binds to KAAG1 on the surface of cancer cells, with LAMP1, a marker of late endosomes/lysosomes, shows that the antibody/antigen complex was internalized and that it follows a pathway that is amenable for the release of a payload that would be conjugated to the 3A4 antibody. Identical results were observed in another cancer cell line, TOV-21G (see Figure SB).

Taken together, these studies demonstrated that dies specific for KAAG1 such as 3A4 might have uses as an antibody-drug conjugate (ADC). Thus, the high level of ovarian cancer specificity of KAAG1 coupled with the capacity of this target to be internalized in cells would support the development of applications as an ADC.

Example 5 Preferential detection of KAAG1 on the surface of cancer cells.

Although l antibodies cting with different epitopes of the KAAG1 protein were developed, the accessibility of these epitopes when KAAG1 is expressed on the surface of intact cancer cells was not fully elucidated. ormatics analysis of the y amino acid structure of KAAG1 (total number of amino acids in the human protein is 84) did not reveal any s sequences that might correspond to a trans- membrane domain and therefore how KAAG1 was anchored to the cell membrane was not fully known.

The antibodies generated against KAAG1 were found to bind to three different regions in the KAAG1 protein (see ). Most of the antibodies interact with amino acids 35 — 60 in the KAAGl protein and are exemplified by antibodies 3D3 and 3612 in this application. Antibodies that interact with the carboxy-terminal end of KAAG1 between amino acids 61 — 84 are exemplified by antibody 3A4. Finally, antibodies that interact with the amino-terminal region of the protein, as exemplified by 304, showed very little binding to cells that express KAAG1.

This ation shows that when KAAG1 is expressed in cells, the carboxy-terminal region (amino acids 61 - 84) is exposed to the extracellular space and that antibodies that target this region are the most efficient at detecting and potentially treating KAAGl-positive cells. Antibodies that bind to the middle region of KAAGl (amino acids 35 — 60) can also detect KAAG1 on the cells surface but to a lesser extent than antibodies that interact with the carboxy-terminus.

Ovarian cancer cell lines such as SKOV-3, are positive for KAAG1 expression. These cells were used to detect the expression KAAG1 by flow cytometry, which is a method that allows the detection of cell surface proteins and is well known by those skilled in the art. Briefly, for each sample 100,000 cells were incubated on ice for 1h with the y antibody (either AAG1, or the control antibody) at a concentration of 1 ug/ml. After several washes with ice-cold PBS, the stained cells were incubated with the secondary antibody that was conjugated to a fluorochrome (FlTC) which detects the presence of the primary antibody bound to the cells. After several additional washes, the cells were analyzed with a flow cytometer. The s expressed in Figure 6 show the Y-axis representing the number of counts (cells) and the X-axis representing the quantity of fluorescence (fluorescence signal). When SKOV-3 cells were incubated with the 3A4 antibody, a large shift in fluorescence was observed indicating that there was nt KAAG1 protein on the surface of the cells (Figure 6A) and that it was efficiently recognized by this antibody. Under identical conditions, the antibodies that interact with the middle region of KAAG1, 3612 and 3D3 (Figure 6A) were significantly less efficacious for detecting KAAGl.

When the cells were incubated with increased concentration of 3612 or 3D3, KAAG1 could be detected on the cell surface (not shown). When the cells were ted with either the l lgG (Figure 6A) or the 3C4, an antibody against the amino terminus of KAAG1 (Figure 6A), no signal was observed. These results indicate that antibodies that interact with the carboxy-terminus of KAAG1 can detect the antigen on the surface of cancer cells more ently then antibodies directed against other regions of KAAG1. This implied that the carboxy-terminus of KAAG1 is d to the extracellular (outside) space of the cell. Similar results were ed for other cancer cell lines that express KAAG1.

The experiment was also med in SKOV-3 cells that were permeabilized with Triton X-100. Triton X-100 is typically used to permeabilize cell membranes and release membrane proteins. When the bilized cells were incubated with 3A4 and measured in the flow cytometer (see Figure 6B), the signal was similar to that obtained in intact cells. ngiy, when the permeabilized cells were ted with the 3G12 antibody that binds to the middle region of KAAG1 (Figure SB), the signal was as strong as the 3A4. These results indicate that the middle region of KAAG1 is likely present in the cell membrane or the inside of the cell. A similar result was obtained with the 303 antibody, another middle-region binder (Figure GB) but the signal obtained for 303 was not as strong. As before, igG control did not show any detectable signal in this assay (Figure 6B). interestingly, incubation of the cells with the 304 antibody which binds to the amino region of KAAG1, did not result in any detectable signal (Figure 6B). This last result suggested that the amino region of KAAG1 is likely cleaved off during the transport of the n to the cell ne.

Overall, these experiments provide much insight into the structure and orientation of the KAAG1 antigen when it is expressed on the surface of cancer cells. Based on these data, two models for the structure of KAAG1 at the cell surface is proposed (Figure 7). In the first model (Figure 7, Model A), the data suggests that the middle portion is actually the transmembrane region of KAAG1 that is only lly exposed to the extra—cellular space. This would make the carboxy-terminus of KAAG1 easily detectable and the middle region more difficult to bind. In the second model (Figure 7, Model B), KAAG1 is anchored to the membrane by another protein that itself is embedded in the cell membrane. Again, the carboxy-terminus would be easily accessible by antibodies such as 3A4 but the ction between KAAG1 and the protein partner would make access to the middle region difficult. The results g that antibodies consisting of both the y—terminai binders (as ified by 3A4) and middle-region binders (as exemplified by 3612 and 3D3) tested in the ce of permeabilized cells is in agreement with both models. The inability of the 304 antibody to bind to KAAG1 in intact or permeabilized cells is likely due to the lack of amino acids contained in the amino-terminus of the mature processed membrane form of KAAG1 and both models are in agreement with this.

These s imply that antibodies that target the carboxy-terminus of KAAG1 in cancer cells, in particular the region spanned by amino acids 61 — 84, are the most appropriate for the development of antibodies for uses as therapeutics for the treatment of carcinomas that express KAAG1. In addition, other uses for the KAAG1 antibodies that bind to the carboxy-terminai region include diagnostic reagents for the detection of carcinomas that express KAAG1.

Antibodies or antigen binding fragments having a binding specificity similar to the 3A4 antibody may be generated or isolated by immunizing an animal with the C- terminal portion of KAAG1 according to methods known in the art, including hybridoma technology, by screening a library of antibody or antigen binding fragments with the C—terminal portion of KAAG1 and/or performing competition assay of isolated antibodies or antigen binding fragment with the 3A4 antibody described herein.

Example 6 Humanization by design of the 3A4 mouse monoclonal dy 3D modeling of the variable s of the mouse 3A4 monoclonal antibody.

This task was accomplished by homology modeling. The most similar template structures to the murine 3A4 variable region sequences of the light and heavy chains (SEQ ID NOs: 4 and 2) were identified by a BLAST search against PDB. To build an initial model of the mouse 3A4 variable region the following template ures were used (PDB codes): 2|PU (chain L) for the light chain, and 1F11 (chain B) for the heavy chain. Other suitable templates can be found in the PDB entry 2DDQ for the light chain, and in the PDB s 3lY3, 1KTR, 2VXT, 1A6T ad 1lGl for the heavy chain. Required mutations were operated on these template structures according to the murine 3A4 sequences: 7 mutations in the 2|PU light chain, and 17 mutations plus a due deletion in the 1F11 heavy chain. The mutated structures corresponding to the heavy and light chains of the murine 3A4 variable regions were assembled into two—chain antibody structures by mposing the heavy and light chains of the tive template structures. The resulting structure of the led 3A4 variable region was first refined by energy zation with the AMBER force— field and a stepwise release of constraints, ranging from the CDR loops that were relaxed first, to the ne heavy atoms of the framework region that were fully d only in the last stage. The CDR-H3 loop in each antibody variable region structure was then refined by Monte-Carlo-minimization (MCM) conformational ng, in which dihedral angles in the CDR-H3 region were sampled in each MCM cycle followed by energy minimization of a predefined region extending 10 A around the initial conformation of the CDR-H3 loop. A entation of the modeled variable region of the mouse 3A4 antibody is given in Figure 8. The structures of the human or humanized variable sequences most similar to each of the 3A4 variable sequences were also identified from PDB, and then superimposed onto the modeled structures of the murine 3A4 variable s. These structures include PDB entries 3QCT, 3AAZ, 1WT5 and 3M80 for the light chain, and PDB s 1|9R, 3NFP, 1T04, 1ZA6, 3HC4, 207T and 1WT5 for the heavy chain. These ures were used to assist in the modeling of mutations in the framework region in order to build the humanized 3D-structures starting from the modeled murine BD-structure.

Characterization of the mouse 3A4 amino-acid sequences and modeled ure.

This step was d out to estimate the humanness index, antigen contact propensity index, to delineate the CDRs, canonical residues, inter-chain packing (VHNL interface residues), variable-/constant—region packing (VH/CH and VL/CL interface residues), unusual framework residues, potential N- and O-glycosylation sites, buried residues, Vernier zone residues, and proximity to CDRs. Internet- available resources and local software were used to assess these properties. ion of the best human chain and heavy-chain frameworks for the mouse CDRs.

This was done by standard sequence gy comparison against a local copy of human germline databases (VBASE), t other sequence libraries (Genbank and SwissProt), as well as the set of human framework consensus sequences. BLAST searches were conducted to retrieve sequence matches with highest homology in the framework region only (thus excluding CDRs) while matching the length of the CDR loops. The human frameworks identified for the light and heavy chains of the 3A4 antibody correspond to the k2 and M classes, respectively. Several human germline framework sequences that are most r to the 3A4 framework sequences were retained in addition to the human sus sequences for these classes.

Identification of framework residues for back-mutations and design of multiple humanized variants.

This is an important step that flags amino-acid residues that should be mutated to the corresponding human sequences with particular care. These residues represent y candidates for back-mutations to the mouse sequences in case of affinity loss. It is the most difficult and unpredictable step of humanization by design, particularly in the absence of an experimental ure of the antibody-antigen complex. It relies on the identification of residues in one or more of the following categories: canonical, CDR—H3, Vernier zone, unusual, CDR-proximal (within 5 A), inter-chain packing, and glycosylation-site residues. Such residues might affect antigen-binding site and affinity directly or indirectly. The antigen contact sity index as well as amino-acid occurrence in human germline databases at each position are also extremely important in deciding whether a certain residue can be safely mutated from the mouse sequence to the human sequence. Humanization of the 3A4 dy light chain variable region involves 11 mutations to its proposed humanized framework for 100% framework humanization. Humanization of the 3A4 antibody heavy chain variable region involves 23 mutations to its proposed humanized framework for 100% framework humanization. These 100% humanized variable region sequences are labelled Lvh1 and th1, respectively (SEQ ID NOsz33 and 38). Additional humanized sequences were also designed in which several residues from the 3A4 mouse sequences were retained based on careful structural and ative sequence analyses that te a high probability of altering antigen-binding affinity if mutations are to be introduced at these positions. These sequences of the variable regions are labelled Lvh2, th2, th3 and th4 (SEQ ID N03: 34, 39, 40 and 41).

The two humanized light chain variants (including the constant region) are fied herein as Lh1 (SEQ ID NO.: 43) and Lh2 (SEQ ID ). The four humanized heavy chain ts (including the constant region_ are identified herein as Hh1 (SEQ ID NO.:46), Hh2 (SEQ ID NO.:47), Hh3 (SEQ ID NO.:48) and HM (SEQ ID NO.:49). The two humanized light chain and 4 humanized heavy chain can be assembled into 8 humanized antibodies (Lh1Hh1, Lh1Hh2, Lh1Hh3, Lh1Hh4, Lh2Hh1, Lh2Hh2, Lh2Hh3, and Lh2Hh4). Molecular models for all these combinations were constructed by homology ng ng from the 3D model of the murine 3A4 le region, and are depicted in Figures 9a-9h.

In the case of 3A4 light-chain humanized ce Lvh2 (SEQ ID NO:34), ork residues Val-L2 and 5 were retained from the mouse sequence since residue L2 is semi—buried, contacts both CDR-L1 and CDR-L3, and has n—contacting propensity, while residue L45 approaches the heavy-chain. We note that both these murine residues may occur in human frameworks. In the case of 3A4 heavy-chain humanized sequence th2 (SEQ ID N0239), framework residues Ile-H2 and Lys-L73 were ed from the mouse sequence since residue H2 is semi-buried, contacts both CDR—H1 and CDR-H3, and has antigen-contacting propensity, while residue H73 belongs to the Vernier zone supporting CDR-H2, and both these murine residues may occur in human frameworks. In the case of 3A4 chain humanized sequence th3 (SEQ ID NO:40), lle-H2 and Lys-L73 back- mutations were retained and in addition to these, framework residues lle-H48, Ala- H67, 9 and Val-H71 were retained from the mouse ce since all these additional murine residues are buried residues and belong to the Vernier zone supporting CDR-H2, and also murine residue H71 may occur in human frameworks.

In the case of 3A4 heavy-chain humanized sequence th4 (SEQ ID NO:41), all 6 back—mutations of the th3 humanized variant were included plus additional two mouse framework residues Lys-H38 and Lys-H66 since they represent semi—buried residues close to . The resulting amino acid sequences of the murine and humanized chains are listed in Table 1. The alignment of the murine and humanized light chain variable regions is shown in Figure 10a and the alignment of the murine and humanized heavy chain variable regions is shown in Figure 10b.

Figure 11a and 11b is an alignment of the murine light chain variable region with the 100% humanized light chain variable region and the murine heavy chain variable region with the 100% humanized heavy chain variable region respectively. This figure illustrates the amino acids that are preserved and those that have been chosen for substitution.

Example 7.

Assembly and expression of 3A4 humanized variant antibodies The purpose of these investigations is to ine the kinetics ters of anti- clusterin antibodies. In particular, to determine whether the humanization of the 3A4 anti-KAAG1 monoclonal antibody s the kinetics parameters of its binding to human KAAG1. To this end, a kinetic analysis method was developed using the ProteOn XPR36 instrument from . Human KAAG1 was immobilized on a sensor chip. Full length antibodies or Fab fragments were injected and allowed to interact with the immobilized KAAG1.

Construction of d encoding the chimeric (murine) heavy and light chains of The heavy and light chains of the ic antibody were amplified by PCR from the original murine immunoglobulin chains using the following oligonucleotide primer pairs: heavy chain, 5’—oligo encoded by SEQ ID NO: 50 and 3’-oligo encoded by SEQ ID NO:51; light chain, 5'-oligo d by SEQ ID NO: 52 and 3’-oligo encoded by SEQ ID NO:53. The ing PCR ts were digested by Hind Ill and cloned into pK-CR5 (SEQ ID NO:21) previously digested with Hind Ill.

Construction of plasmids encoding the zed heavy chain 3A4 ts 1, 2, 3 and 4 The fragments coding for the humanized heavy chain region of the antibody 3A4 (Hh1, Hh2, Hh3 and HM) were ordered from GenScript (Piscataway, USA). The DNA fragments including the kozak and stop codon sequences were digested with Hindlll and cloned into the Hindlll site of plasmid pK-CR5 previously dephosphorylated with calf intestinal phosphatase (NEB) to prevent recircularization.

Figure 12a shows the map of the plasmid -3A4-HC-variant1. All heavy chain variants of the humanized 3A4 were constructed in a similar manner.

Construction of plasmids ng the zed light chain 3A4 variants 1 and 2 The fragments coding for the human light chain regions of the antibody 3A4 (Lh1 and Lh2) were ordered from GenScript. The DNA fragments including the kozak and stop codon sequences was digested with BamHl and cloned into the BamHl site of plasmid pMPG-CR5 (SEQ lD NO:55) previously dephosphorylated with calf intestinal phosphatase (NEB) to prevent recircularization. Figure 12b shows the map of the plasmid pMPG-CR5-3A4-LC-variant1. All light chain variants of the zed 3A4 were constructed in a similar manner.

Transient transfection study d DNA was isolated from small cultures of E. coli using the Mini-Prep kit (Qiagen Inc, sauga, ON) according to the manufacturer’s recommendation.

Briefly, 2 ml of LB medium containing 100 ug/ml of ampicillin were inoculated with a single colony picked after ligation and transformation. The cultures were incubated at 37°C overnight with vigorous shaking (250 RPM). The plasmid was then isolated from 1.5 ml of culture using the protocols, buffers, and columns provided by the kit.

The DNA was eluted using 50 ul of sterile water. Plasmid DNA was isolated from large culture of E. coli using the Plasmid Plus Maxi kit (Qiagen Inc, Mississauga, ON) according to the manufacturer’s endation. 200 mL of LB medium containing 100 ug/mL ampicillin were inoculated with a single fresh colony of E. coli and incubated overnight at 37°C with vigorous shaking (250 RPM). The bacteria (130 mL of culture for the heavy chain and 180 mL of culture for the light chain) were pelleted by centrifugation at 6000 x g, for 15 min, at 4°C and the plasmid was isolated using the protocols, buffers and columns provided by the kit. The pure plasmids was resuspended in e 50 mM Tris, pH8 and quantified by measuring the optical density at 260 nm. Before transfection the purified plasmid were sterilized by extraction with phenol/chloroform followed by ethanol precipitation. The plasmid were resuspended in sterile 50 mM Tris, pH 8 and quantified by optical density at 260 nm.

Before ection, the cells (CHO-cTA) were washed with PBS and ended at a concentration of 4.0 X 106 cell/ml in growth medium (CD-CHO, lnvitrogen) without dextran sulfate for 3 h in suspension e. For each plasmid combination, 45 ml of cells were ected by adding slowly 5 ml of CDCHO medium supplemented with pg/ml of each plasmid and 50 pg/ml of polyethylenimine (PEI Max; Polysciences).

The final concentration was 1 pg/ml of each plasmid and 5 ug/m1 of PEI. After 2 h, the cells were erred at 30°C. The next days, 50 ug/mL of dextran sulfate and 3.75 ml of each supplement (Efficient Feed A and B lnvitrogen) were added to the cells and they were incubated at 30°C for 13 days. 2.5 ml of Feed A and 2.5 ml of Feed B were added at day 4, 6, 8 and 11. On day 13, the supernatant was clarified by centrifugation and filtered through a 0.22 pM .

CHO cells (CHOcTA) were transfected with plasmids encoding the different variants of humanized heavy and light chains of the 3A4 antibody regulated by the CR5 promoter. Transfection with different combinations of light and heavy chains was performed. As control, cells were also transfected with ds ng the chimeric/murine antibody.

Purification of antibody ml of supernatant from the CH0 cell transfections were concentrated by centrifugation using the Amicon Ultra (Ultacell-50k) cassette at 1500 rpm. The concentrated antibody (550 pl) was purified using the Nab spin kit Protein A Plus (Thermo Scientific) according to the manufacture’s endations. The purified antibodies were then desalted using PBS and the concentrating Amicon Ultra (UltraceI-10K) cassette at 2500 rpm to a final volume of 250 pl. The purified antibody was quantified by reading the ODzeo using the op spectrophotometer and kept frozen at -20°C. An aliquote of the purified antibody was resuspended into an equal volume of Laemmli 2X and heated at 95°C for 5 min and chilled on ice. A standard curve was made using known amount of purified human lgG1 kappa from Human Myeloma plasma (Athens Research). The samples were separated on a polyacrylamide Novex 10% Tris-Glycine gel (lnvitrogen Canada Inc, Burlington, ON) and transferred onto a Hybond-N nitrocellulose membrane ham ence Corp, Baie e, QC) for 1 h at 275 mA. The membrane was blocked for 1 h in 0.15% Tween 20, 5% skimmed milk in PBS and incubated for 1 hr with an Goat anti- Human lgG (H+L) conjugated to Cy5 (Jackson, Cat# 109—176-099). The signal was revealed and quantified by scanning with the Typhoon Trio+ scanner (GE Healtcare).

As shown in Figure 13, all combinations of the 3A4 humanized antibody variants were sed in CHO cells.

Example 8.

Kinetic analysis of murine and humanized 3A4 antibody Supplies GLM sensorchips, the Biorad ProteOn amine coupling kit (EDC, sNHS and ethanolamine), and 10mM sodium acetate buffers were purchased from Bio-Rad Laboratories (Mississauga, ON). HEPES buffer, EDTA, and NaCl were purchased from from Sigma-Aldrich lle, ON). Ten percent Tween 20 solution was purchased from Teknova (Hollister, CA). The goat anti-human lgG Fc fragment specific antibody was purchased from Jackson ImmunoResearch. The gel filtration column Superdex 75 10/300 GL was purchased from GE care.

Gel filtration The KAAG1 protein at a concentration of 3.114 mg/ml and a volume of 220 uL was injected onto the Superdex G75 column. The separation was done at 0.4ml/min in HBST running buffer (see below) without Tween 20. The volume of the fractions collected was 500 uL. Concentration of KAAG1 in each fractions was determined by OD 280 using an extension coefficient of 5500 and a MW of 8969. Figure 14 represents the e of the gel filtration of KAAG1. A small peak of potential aggregate is eluting at around 11 ml. The protein g at 13 ml was used as e for the SPR assay (fractions 15 — 19).

SPR biosensor assays All surface plasmon resonance assays were carried out using a BioRad ProteOn XPR36 instrument (Bio-Rad Laboratories Ltd. ssauga, ON) with HBST running buffer (10mM HEPES, 150 mM NaCl, 3.4 mM EDTA, and 0.05% Tween 20 pH 7.4) at a ature of 25°C. The anti-mouse Fc capture surface was generated using a GLM sensorchip activated by a 1:5 dilution of the standard BioRad sNHS/EDC solutions injected for 300 s at 30 uL/min in the analyte (horizontal) direction.

Immediately after the activation, a 13 ug/mL solution of anti-human lgG Fc fragment specific in 10 mM NaOAc pH 4.5 was injected in the analyte direction at a flow rate of uL/min until approximately 8000 resonance units (RUs) were immobilized.

Remaining active groups were quenched by a 300 3 injection of 1M ethanolamine at uL/min in the analyte ion, and this also ensures mock—activated pots are created for blank referencing. The screening of the 3A4 variants for binding to KAAG1 occurred in two steps: an indirect capture of 3A4 variants from cell supernatant onto the anti-human lgG Fc fragment specific surface in the ligand direction(vertical) followed by a KAAG1 injection in the analyte direction. Firstly, one buffer ion for 30 s at 100 uL/min in the ligand direction was used to stabilize the baseline. For each 3A4 capture, unpurified 3A4 ts in cell-culture media were diluted to 4 °/o in HBST, or approximately 1.25 pg/mL of purifed 3A4 in HBST was used. Four to five 3A4 ts along with wild-type 3A4 were simultaneously injected in individual ligand channels for 240 s at flow 25 pL/min. This resulted in a saturating 3A4 capture of approximately 400-700 RUs onto the uman lgG Fc fragment specific surface. The first ligand l was left empty to use as a blank control if required. This 3A4 capture step was immediately followed by two buffer injections in the analyte direction to stabilize the baseline, and then the gel filtration purified KAAG1 was injected. For a typical screen, five KAAG1 concentrations (8, 2.66, 0.89, 0.29, and 0.098 nM) and buffer control were simultaneously injected in individual analyte channels at 50 uL/min for 120 s with a 600s dissociation phase, resulting in a set of binding sensorgrams with a buffer reference for each of the captured 3A4 variants. The anti-human lgG Fc fragment specific — 3A4 xes were regenerated by a 18 8 pulse of 0.85% phosphoric acid for 18 s at 100 pL/min to prepare the anti-human lgG Fc fragment specific surface for the next injection cycle.

Sensorgrams were aligned and double-referenced using the buffer blank ion and interspots, and the ing sensorgrams were analyzed using ProteOn Manager software v3.0. The c and affinity values were determined by fitting the referenced sensorgrams to the 1:1 Langmuir binding model using local Rmax, and affinity constants (KD M) were derived from the resulting rate constants (kd s'1/ k2,1 M'1s' Determination of rate and affinity constants Figure 15 summarizes the association (k3, 1/Ms) and dissociation (kd, 1/s) rate constants as well as affinity (KD, M) nts for the interaction of KAAG1 with purified murine 3A4, murine 3A4 transiently expressed as a chimeric and transiently expressed zed variants. These constants are graphically represented in Figure 16. The ation rate constant is very similar for the pure parental, chimeric and zed 3A4 variants (Figure 16a). The dissociation rate constants is similar for the transiently express chimeric as ed to the pure parental 3A4 with suggest that the transfection procedure did not alter the parameters of the interaction of KAAG1 with the antibody (Figure 16b). However all zed variants seem to have a slightly altered off rate, i.e. quicker dissociation rate (Figure 16b). This is reflected in the affinity constants (Figure 160). In summary, there is a linear correlation between the binding affinity (logKD) of the humanized variant and the number of back-mutations made in the parent antibody (Lch) with a decrease in the g affinity as the number of mutations is increasing. However, the difference in binding affinity is only 4 fold different between the worse variant (H1L1, 0.47 nM) which has no mouse residue retained and the best variant which has 10 mouse residues retained (H4L2, 0.1 nM). Finally, the binding affinity of all variants for KAAG1 was found to be sub-nanomolar and the best variant (H4L2, 0.1 nM) exhibited an affinity about 6-fold weaker than the murine (Lch, 0.057 nM). Overall, these results indicate that humanization was successful as all of the variants displayed very high affinity for KAAG1.

Example 9.

Binding of 3A4 humanized variants to KAAG1 in an ELISA ELISA methods were also used to e the g activity of the zed 3A4 variants to the murine 3A4 antibody. Recombinant human KAAG1 was coated in 96- well plates O/N, washed and incubated for 1h at RT with increasing quantities of murine or humanized 3A4 variants. Following another round of washing steps, an anti-human antibody conjugated to HRP was added to the wells and the bound 3A4 antibody was measured metrically at Abs450. As shown in Figure 17A, the humanized variants (Lh1Hh1, Lh1Hh2, Lh1Hh3 and Lh1Hh4) displayed very similar binding to KAAG1 when compared to the murine 3A4 (Lch). This result ted that all four zed heavy chain variants were comparable to the original h3A4 heavy chain when assembled with the L1 variant of the humanized light chain. Figure 178 shows the results when the heavy chain variants were assembled with Lh2 variant of the 3A4 humanized light chain. In this instance, there was a difference in the binding of the variants. For example, Lh2hh4 was the variant with the closest profile compared to the murine 3A4. This was in agreement with the SPR data (see Example 3), which showed that the variant 4 of the heavy chain had the highest affinity for KAAG1. Taken together, these binding results show that the humanized variants all interact with human KAAG1 in this assay. Although there were some subtle differences, the binding in ELISA was in concordance with the SPR results.

Example 10. g of 3A4 humanized variants on the surface of cancer cells Flow cytometry was used to evaluate the capacity of the humanized 3A4 variants to ct with KAAG1 expressed on the surface of cancer cells. To this end, SKOV-3 ovarian cancer cells, which we had previously showed were efficiently bound by 3A4 by flow try, were incubated with the eight zed variants and the original murine antibody. Briefly, SKOV-3 cells were detached from the plate with EDTA and incubated on ice with either 3.0 mg/ml, 0.3 mg/ml or 0.3 mg/ml of the antibodies for 1h. After three washing steps, the cells were incubated with the secondary antibody, anti-human lgG-conjugated to FITC for 1h on ice. Cell surface fluorescence was measured in a flow cytometer and the values ae shown in the histogram of Figure 18.

As depicted, all variants could detect KAAG1 on the surface on unpermeabilized and the est s were obtained at the highest concentration of 3A4 dies (3 mg/ml) and decreased as the concentration of the antibody was decreased. Among the different variants, the ones with the most murine back-mutations (Figure 18, see Lh1Hh4 and Lh2Hh4) interacted with KAAG1 on the surface of cells with the highest activity. In fact, Lh1Hh4 and Lh2hh4 appeared to be slight improved cell surface binding to KAAG1 compared to the murine 3A4 antibody (Lch).

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Sequences referred to in the description SEQ lD NO.:1 — 3A4 heavy chain variable region nucleotide sequence CAGATCCAGTTGGTGCAATCTGGACCTGAGATGGTGAAGCCTGGGGCTTCAGTGAAGATGTCCTGTAAG GCTTCTGGATACACATTCACTGACGACTACATGAGCTGGGTGAAACAGAGCCATGGAAAGAGCCTTGAG TGGATTGGAGATATTAATCCTTACAACGGTGATACTAACTACAACCAGAAGTTCAAGGGCAAGGCCATA TTGACTGTAGACAAATCCTCCAGCACAGCCTACATGCAGCTCAACAGCCTGACATCGGAAGACTCAGCA GTCTATTACTGTGCAAGAGACCCGGGGGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCC SEQ ID NO.:2 — 3A4 heavy chain variable region polypeptide sequence QIQLVQSGPEMVKPGASVKMSCKASGYTFTDDYMSWVKQSHGKSLEWIGDINPYNGDTNYNQKFKGKAI LTVDKSSSTAYMQLNSLTSEDSAVYYCARDPGAMDYWGQGTSVTVSS SEQ lD NO.:3 — 3A4 light chain variable region nucleotide sequence GATGTTGTGATGACCCAAACTCCACTCTCCCTGGCTGTCAGTCTTGGAGATCAAGCCTCCATCTCTTGC AGATCTAGTCAGAGCCTTCTACATAGTAATGGAAACACCTATTTAGAATGGTACCTTCAGAAACCAGGC CAGTCTCCAAAGCTCCTGATCCACACAGTTTCCAACCGATTTTCTGGGGTCCCAGACAGATTCAGTGGC AGTGGATCAGGGACAGATTTCACACTCAAGATCAGCAGAGTGGAGGCTGAGGATCTGGGAGTTTATTAC CAAGGTTCACATGTTCCGCTCACGTTCGGTGCTGGGACCAGGCTGGAGCTGAAA SEQ ID NO.:4 — 3A4 light chain variable region polypeptide sequence DVVMTQTPLSLAVSLGDQASISCRSSQSLLHSNGNTYLEWYLQKPGQSPKLLIHTVSNRFSGVPDRFSG FTLKISRVEAEDLGVYYCFQGSHVPLTFGAGTRLELK SEQ ID NO.:5 — 3A4 heavy chain CDR1 polypeptide ce GYTFTDDYMS SEQ ID NO.:6 — 3A4 heavy chain CDR2 polypeptide sequence GDTN SEQ ID NO.:7 — 3A4 heavy chain CDR3 polypeptide sequence DPGAMDY SEQ ID NO.:8 — 3A4 light chain CDR1 polypeptide sequence RSSQSLLHSNGNTYLE SEQ lD NO.:9 — 3A4 light chain CDR2 polypeptide sequence TVSNRFS SEQ ID NO.:10 — 3A4 light chain CDR3 polypeptide sequence FQGSHVPLT SEQ ID NO.:11 — OGS1773 GTAAGCAGCGCTGTGGCTGCACCATCTGTCTTC SEQ ID NO.:12 — OGS1774 GTAAGCGCTAGCCTAACACTCTCCCCTGTTGAAGC SEQ ID NO.:13 — human kappa constant nucleotide ce GCTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCT GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC CAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGC ACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGC CTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAG SEQ ID NO.:14 — human kappa nt polypeptide sequence AVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSS TLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC SEQ ID NO.:15 CTTGAGCCGGCGGATGGTCGAGGTGAGGTGTGGCAGGCTTGAGATCCAGCTGTTGGGGTGAGTACTCCC TCTCAAAAGCGGGCATTACTTCTGCGCTAAGATTGTCAGTTTCCAAAAACGAGGAGGATTTGATATTCA CCTGGCCCGATCTGGCCATACACTTGAGTGACAATGACATCCACTTTGCCTTTCTCTCCACAGGTGTCC ACTCCCAGGTCCAAGTTTAAACGGATCTCTAGCGAATTCATGAACTTTCTGCTGTCTTGGGTGCATTGG AGCCTTGCCTTGCTGCTCTACCTCCACCATGCCAAGTGGTCCCAGGCTTGAGACGGAGCTTACAGCGCT GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTT GTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAA TCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC CTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTG AGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGGGTACCGCGGCCGCTTCGAATGAGATC CCCCGACCTCGACCTCTGGCTAATAAAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAATTTTTTGT GTCTCTCACTCGGAAGGACATATGGGAGGGCAAATCATTTGGTCGAGATCCCTCGGAGATCTCTAGCTA GAGCCCCGCCGCCGGACGAACTAAACCTGACTACGGCATCTCTGCCCCTTCTTCGCGGGGCAGTGCATG TAATCCCTTCAGTTGGTTGGTACAACTTGCCAACTGGGCCCTGTTCCACATGTGACACGGGGGGGGACC AAACACAAAGGGGTTCTCTGACTGTAGTTGACATCCTTATAAATGGATGTGCACATTTGCCAACACTGA TTCATCCTGGAGCAGACTTTGCAGTCTGTGGACTGCAACACAACATTGCCTTTATGTGTAACT CTTGGCTGAAGCTCTTACACCAATGCTGGGGGACATGTACCTCCCAGGGGCCCAGGAAGACTACGGGAG GCTACACCAACGTCAATCAGAGGGGCCTGTGTAGCTACCGATAAGCGGACCCTCAAGAGGGCATTAGCA ATAGTGTTTATAAGGCCCCCTTGTTAACCCTAAACGGGTAGCATATGCTTCCCGGGTAGTAGTATATAC TATCCAGACTAACCCTAATTCAATAGCATATGTTACCCAACGGGAAGCATATGCTATCGAATTAGGGTT AGTAAAAGGGTCCTAAGGAACAGCGATATCTCCCACCCCATGAGCTGTCACGGTTTTATTTACATGGGG TCAGGATTCCACGAGGGTAGTGAACCATTTTAGTCACAAGGGCAGTGGCTGAAGATCAAGGAGCGGGCA GTGAACTCTCCTGAATCTTCGCCTGCTTCTTCATTCTCCTTCGTTTAGCTAATAGAATAACTGCTGAGT TGTGAACAGTAAGGTGTATGTGAGGTGCTCGAAAACAAGGTTTCAGGTGACGCCCCCAGAATAAAATTT GGACGGGGGGTTCAGTGGTGGCATTGTGCTATGACACCAATATAACCCTCACAAACCCCTTGGGCAATA AATACTAGTGTAGGAATGAAACATTCTGAATATCTTTAACAATAGAAATCCATGGGGTGGGGACAAGCC GTAAAGACTGGATGTCCATCTCACACGAATTTATGGCTATGGGCAACACATAATCCTAGTGCAATATGA TACTGGGGTTATTAAGATGTGTCCCAGGCAGGGACCAAGACAGGTGAACCATGTTGTTACACTCTATTT GTAACAAGGGGAAAGAGAGTGGACGCCGACAGCAGCGGACTCCACTGGTTGTCTCTAACACCCCCGAAA ATTAAACGGGGCTCCACGCCAATGGGGCCCATAAACAAAGACAAGTGGCCACTCTTTTTTTTGAAATTG TGGAGTGGGGGCACGCGTCAGCCCCCACACGCCGCCCTGCGGTTTTGGACTGTAAAATAAGGGTGTAAT AACTTGGCTGATTGTAACCCCGCTAACCACTGCGGTCAAACCACTTGCCCACAAAACCACTAATGGCAC CCCGGGGAATACCTGCATAAGTAGGTGGGCGGGCCAAGATAGGGGCGCGATTGCTGCGATCTGGAGGAC CACACACTTGCGCCTGAGCGCCAAGCACAGGGTTGTTGGTCCTCATATTCACGAGGTCGCTGA GAGCACGGTGGGCTAATGTTGCCATGGGTAGCATATACTACCCAAATATCTGGATAGCATATGCTATCC TAATCTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATA TCTGGGTAGTATATGCTATCCTAATTTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAG CTATCCTAATCTATATCTGGGTAGTATATGCTATCCTAATCTGTATCCGGGTAGCATATGCTA TCCTAATAGAGATTAGGGTAGTATATGCTATCCTAATTTATATCTGGGTAGCATATACTACCCAAATAT CTGGATAGCATATGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGC ATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGCTAT CCTAATTTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTA TATCTGGGTAGTATATGCTATCCTAATCTGTATCCGGGTAGCATATGCTATCCTCACGATGATAAGCTG TCAAACATGAGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTC ATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAA GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG GCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC ACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAC TCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTT GATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAG ATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC AGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTA GCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGT CTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCG TGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAA AGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAG AGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGA CTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCC TTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCT GTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGC GAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATT CATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTG AGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCTAGCTAGAGGTC GACCAATTCTCATGTTTGACAGCTTATCATCGCAGATCCGGGCAACGTTGTTGCATTGCTGCAGGCGCA GAACTGGTAGGTATGGCAGATCTATACATTGAATCAATATTGGCAATTAGCCATATTAGTCATTGGTTA ATAAATCAATATTGGCTATTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTA TATTGGCTCATGTCCAATATGACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAAT TACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCC TGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAAT AGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGT GTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCA GTACATGACCTTACGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGT GATGCGGTTTTGGCAGTACACCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCA CCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAA CCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTT AGTGAACCGTCAGATCCTCACTCTCTTCCGCATCGCTGTCTGCGAGGGCCAGCTGTTGGGCTCGCGGTT GAGGACAAACTCTTCGCGGTCTTTCCAGTACTCTTGGATCGGAAACCCGTCGGCCTCCGAACGGTACTC CGCCACCGAGGGACCTGAGCGAGTCCGCATCGACCGGATCGGAAAACCTCTCGAGAAAGGCGTCTAACC AGTCACAGTCGCAAGGTAGGCTGAGCACCGTGGCGGGCGGCAGCGGGTGGCGGTCGGGGTTGTTTCTGG CGGAGGTGCTGCTGATGATGTAATTAAAGTAGGCGGT SEQ ID NO.:16 — OGS18500 ATGCCAAGTGGTCCCAGGCTGATGTTGTGATGACCCAAACTCC SEQ ID NO:.17 — 4 GGGAAGATGAAGACAGATGGTGCAGCCACAGTCCG SEQ ID NO.:18 — OGS1769 GTAAGCGCTAGCGCCTCAACGAAGGGCCCATCTGTCTTTCCCCTGGCCCC SEQ ID NO.:19 — OGS1770 GTAAGCGAATTCACAAGATTTGGGCTCAACTTTCTTG SEQ ID NO.:20 — human globulin CH1 region nucleotide sequence GCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCA GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTG ACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTG ACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACC AAGGTGGACAAGAAAGTTGAGCCCAAATCTTGT SEQ ID NO.:21 — human immunoglobulin CH1 region ptide sequence ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVV TVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC VOJZZ CTTGAGCCGGCGGATGGTCGAGGTGAGGTGTGGCAGGCTTGAGATCCAGCTGTTGGGGTGAGTACTCCC TCTCAAAAGCGGGCATTACTTCTGCGCTAAGATTGTCAGTTTCCAAAAACGAGGAGGATTTGATATTCA CCTGGCCCGATCTGGCCATACACTTGAGTGACAATGACATCCACTTTGCCTTTCTCTCCACAGGTGTCC ACTCCCAGGTCCAAGTTTGCCGCCACCATGGAGACAGACACACTCCTGCTATGGGTACTGCTGCTCTGG GTTCCAGGTTCCACTGGCGGAGACGGAGCTTACGGGCCCATCTGTCTTTCCCCTGGCCCCCTCCTCCAA GAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGT GTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACT CTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGT GAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGAATTCACTCACAC ATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAA GGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCC TGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGA GCAGTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAA GGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAA GCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGT CAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCA GCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAA GCTCACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCT GCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCCGGGAAATGATCCCCCGACCTCGACCTCTG GCTAATAAAGGAAATTTATTTTCATTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGA CATATGGGAGGGCAAATCATTTGGTCGAGATCCCTCGGAGATCTCTAGCTAGAGCCCCGCCGCCGGACG AACTAAACCTGACTACGGCATCTCTGCCCCTTCTTCGCGGGGCAGTGCATGTAATCCCTTCAGTTGGTT GGTACAACTTGCCAACTGAACCCTAAACGGGTAGCATATGCTTCCCGGGTAGTAGTATATACTATCCAG ACTAACCCTAATTCAATAGCATATGTTACCCAACGGGAAGCATATGCTATCGAATTAGGGTTAGTAAAA GGGTCCTAAGGAACAGCGATGTAGGTGGGCGGGCCAAGATAGGGGCGCGATTGCTGCGATCTGGAGGAC AAATTACACACACTTGCGCCTGAGCGCCAAGCACAGGGTTGTTGGTCCTCATATTCACGAGGTCGCTGA GAGCACGGTGGGCTAATGTTGCCATGGGTAGCATATACTACCCAAATATCTGGATAGCATATGCTATCC TAATCTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATA TCTGGGTAGTATATGCTATCCTAATTTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAG CATATGCTATCCTAATCTATATCTGGGTAGTATATGCTATCCTAATCTGTATCCGGGTAGCATATGCTA TCCTAATAGAGATTAGGGTAGTATATGCTATCCTAATTTATATCTGGGTAGCATATACTACCCAAATAT CTGGATAGCATATGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGC ATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGCTAT CCTAATTTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTA TATCTGGGTAGTATATGCTATCCTAATCTGTATCCGGGTAGCATATGCTATCCTCACGATGATAAGCTG TCAAACATGAGAATTAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTC ATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAA CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG GCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC ACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAC TCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTT GATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAG ATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGC AGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTA GCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGT CTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCG TGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAA AGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAG CGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGA CGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCC CGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCT GTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGC GAGTCAGTGAGCGAGGAAGCGTACATTTATATTGGCTCATGTCCAATATGACCGCCATGTTGACATTGA ACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGC GTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATA ATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGG TAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGAC GGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTGGCAGTACATC TACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACACCAATGGGCGTGGATAGCGG TTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAAT CAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGG TGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCCTCACTCTCTTCCGCATCGCTGTC TGCGAGGGCCAGCTGTTGGGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCAGTACTCTTGGATC CCGTCGGCCTCCGAACGGTACTCCGCCACCGAGGGACCTGAGCGAGTCCGCATCGACCGGATC GGAAAACCTCTCGAGAAAGGCGTCTAACCAGTCACAGTCGCAAGGTAGGCTGAGCACCGTGGCGGGCGG CAGCGGGTGGCGGTCGGGGTTGTTTCTGGCGGAGGTGCTGCTGATGATGTAATTAAAGTAGGCGGT SEQ 23 — OGS1879 GGGTTCCAGGTTCCACTGGCCAGATCCAGTTGGTGCAATCTGG EQ ID NO.:24 — OGS1810 GGGGCCAGGGGAAAGACAGATGGGCCCTTCGTTGAGGC SEQ ID NO.:25 GTAAGCGGATCCATGGATGACGACGCGGCGCCC SEQ ID NO.:26 GTAAGCAAGCTTAGGCCGCTGGGACAGCGGAGGTGC SEQ ID NO.:27 GTAAGCAAGCTTGGCAGCAGCGCCAGGTCCAGC SEQ ID NO.:28 .

GAGGGGCATCAATCACACCGAGAAGTCACAGCCCCTCAACCACTGAGGTGTGGGGGGGTAGGGATCTGC TCATATCAACCCCACACTATAGGGCACCTAAATGGGTGGGCGGTGGGGGAGACCGACTCACTT GAGTTTCTTGAAGGCTTCCTGGCCTCCAGCCACGTAATTGCCCCCGCTCTGGATCTGGTCTAGCTTCCG GATTCGGTGGCCAGTCCGCGGGGTGTAGATGTTCCTGACGGCCCCAAAGGGTGCCTGAACGCCGCCGGT CACCTCCTTCAGGAAGACTTCGAAGCTGGACACCTTCTTCTCATGGATGACGACGCGGCGCCCCGCGTA GAAGGGGTCCCCGTTGCGGTACACAAGCACGCTCTTCACGACGGGCTGAGACAGGTGGCTGGACCTGGC GCTGCTGCCGCTCATCTTCCCCGCTGGCCGCCGCCTCAGCTCGCTGCTTCGCGTCGGGAGGCACCTCCG CTGTCCCAGCGGCCTCACCGCACCCAGGGCGCGGGATCGCCTCCTGAAACGAACGAGAAACTGACGAAT CCACAGGTGAAAGAGAAGTAACGGCCGTGCGCCTAGGCGTCCACCCAGAGGAGACACTAGGAGCTTGCA GGACTCGGAGTAGACGCTCAAGTTTTTCACCGTGGCGTGCACAGCCAATCAGGACCCGCAGTGCGCGCA CCACACCAGGTTCACCTGCTACGGGCAGAATCAAGGTGGACAGCTTCTGAGCAGGAGCCGGAAACGCGC GGGGCCTTCAAACAGGCACGCCTAGTGAGGGCAGGAGAGAGGAGGACGCACACACACACACACACACAA ATATGGTGAAACCCAATTTCTTACATCATATCTGTGCTACCCTTTCCAAACAGCCTA SEQ ID NO.:29 MDDDAAPRVEGVPVAVHKHALHDGLRQVAGPGAAAAHLPRWPPPQLAASRREAPPLSQRPHRTQGAGSP PETNEKLTNPQVKEK SEQ ID NO.:30 (variant light chain variable region) DXVMTQTPLSLXVXXGXXASISCRSSQSLLHSNGNTYLEWYLQKPGQSPXLLIHTVSNRFSG VPDRFSGSGSGTDFTLKlSRVEAEDXGWYCFQGSHVPLTFGXGTXLEXK wherein at least one of the amino acids identified by X is an amino acid substitution rvative or non-conservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ ID NO.:4. The amino acid substitution may be, for example conservative.

SEQ ID NO.:31 (variant light chain variable region) DXa1VMTQTPLSLXaZVXa3Xa4GX35XasASISCRSSQSLLHSNGNTYLEWYLQKPGQSPXa7LLIHT VSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDXaaGVYYCFQGSHVPLTFGXagGTXa1oLEXa11 n X31 may be a hobic amino acid; Wherein Xag may be A or P; Wherein X33 may be neutral hydrophilic amino acid; Wherein Xa4 may be L or P; Wherein X35 may be an acidic amino acid; Wherein X35 may be Q or P; n X37 may be a basic amino acid; Wherein X38 may be a hydrophobic amino acid; Wherein X39 may be A or Q; Wherein X310 may be a basic amino acid; or Wherein X311 may be a hobic amino acid, wherein at least one of the amino acid identified by X is an amino acid substitution (conservative or non-conservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ ID NO.:4.

SEQ ID NO.:32 (variant light chain variable region) DXA1VMTQTPLSLXAZVXA3XA4GXA5XA5ASISCRSSQSLLHSNGNTYLEWYLQKPGQSPXMLLIH TVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDXAaGWYCFQGSHVPLTFGXAgGTXA1OLEXA Wherein XA1 may be V or I Wherein XA2 may be A or P Wherein XA3 may be S or T Wherein XA4 may be L or P Wherein XA5 may be D or E Wherein XAG may be Q or P n XA7 may be K or Q Wherein XAB may be L or V Wherein XAg may be A or Q Wherein XA10 may be R or K or Wherein XA11 may be L or I, wherein at least one of the amino acid identified by X is an amino acid substitution rvative or non-conservative) in comparison with a ponding amino acid in the polypeptide set forth in SEQ ID NO.:4.

SEQ ID NO.:33 (variant 1 light chain variable region: Lvh1) DIVMTQTPLSLPVTPGEPAS|SCRSSQSLLHSNGNTYLEWYLQKPGQSPQLLIYTVSNRFSGV PDRFSGSGSGTDFTLKISRVEAEDVGWYCFQGSHVPLTFGQGTKLEIK SEQ ID NO.:34 (variant 2 light chain variable region: Lvh2) DWMTQTPLSLPVTPGEPASISCRSSQSLLHSNGNTYLEWYLQKPGQSPKLLIYTVSNRFSG VPDRFSGSGSGTDFTLKlSRVEAEDVGWYCFQGSHVPLTFGQGTKLEIK SEQ ID NO.:35 (variant heavy chain variable region) QXQLVQSGXEXXKPGASVKXSCKASGYTFTDDYMSWVXQXXGXXLEWXGDINPYNGDTNY NQKFKGXXXXTXDXSXSTAYMXLXSLXSEDXAVYYCARDPGAMDYWGQGTXVTVSS wherein at least one of the amino acid identified by X is an amino acid substitution (conservative or non-conservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ ID N02. The amino acid substitution may be, for example conservative.

SEQ ID NO.:36 (variant heavy chain le ) QXmQLVQSGszEXb3Xb4KPGASVKXb5SCKASGYTFTDDYMSWVszQXWngGngXmoLEWXb 11GDINPYNGDTNYNQKFKGXM2Xb13Xb14Xb15TXb15DXWSXD188TAYMXb19LszoSLXb21SEDsz 2AVYYCARDPGAMDYWGQGTXmVTVSS Wherein Xm may be a hydrophobic amino acid; Wherein sz may be P or A; Wherein Xb3 may be a hydrophobic amino acid; Wherein Xb4 may be V or K; Wherein Xb5 may be a hydrophobic amino acid; Wherein sz may be a basic amino acid; Wherein Xb7 may be S or A; Wherein ng may be H or P; Wherein ng may be a basic amino acid; Wherein Xmo may be S or G; Wherein an may be a hydrophobic amino acid; Wherein Xm may be a basic amino acid; Wherein Xm may be a hydrophobic amino acid; Wherein XW may be i or T; Wherein Xb15 may be a hydrophobic amino acid; Wherein Xma may be a hydrophobic amino acid; Wherein Xb17 may be K or T; Wherein Xm may be a neutral hydrophilic amino acid; Wherein Xb19 may be Q or E; Wherein ngo may be N or S; n Xb21 may be T or R; Wherein Xm may be a neutral hydrophilic amino acid; or Wherein sza may be S or L, wherein at least one of the amino acid identified by X is an amino acid substitution (conservative or non-conservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ ID NO.:2.

SEQ ID NO.:37 nt heavy chain variable region) QXB1QLVQSGXBZEX33XB4KPGASVKXB5SCKASGYTFTDDYMSWVXBGQXB7XBBGX39XB10LEW X311GDINPYNGDTNYNQKFKGXB12XB13XB14XB15TXB16DXB17SXB188TAYMXB19LXBZOSLX321SE DXBzzAWYCARDPGAMDYWGQGTXmVTVSS n X31 may be I or V; Wherein X32 may be P or A; Wherein X33 may be M or V; Wherein X34 may be V or K; Wherein X35 may be M or V; Wherein X35 may be K or R; Wherein X37 may be S or A; Wherein X38 may be H or P; Wherein X39 may be K or Q; Wherein X510 may be S or G; Wherein X311 may be i or M; Wherein X312 may be K or R; Wherein X313 may be A or V; Wherein X314 may be I or T; n X315 may be L or I; Wherein X316 may be V or A; Wherein X517 may be K or T; Wherein X313 may be S or T; Wherein X319 may be Q or E; Wherein X320 may be N or S; Wherein X321 may be T or R; Wherein X322 may be S or T; or Wherein X323 may be S or L, wherein at least one of the amino acid identified by X is an amino acid substitution (conservative or non-conservative) in comparison with a corresponding amino acid in the polypeptide set forth in SEQ ID NO.:2.

SEQ ID NO.:38 (variant 1 heavy chain variable region: th1) QVQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWMGDINPYNGDTN YNQKFKGRVTITADTSTSTAYMELSSLRSEDTAWYCARDPGAMDYWGQGTLVTVSS SEQ ID NO.:39 (variant 2 heavy chain variable region: th2) Q|QLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWMGDINPYNGDTNY RVTITADKSTSTAYMELSSLRSEDTAWYCARDPGAMDYWGQGTLVTVSS SEQ ID NO.:40 (variant 3 heavy chain variable region: th3) QIQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWIGDINPYNGDTNY NQKFKGRATLTVDKSTSTAYMELSSLRSEDTAWYCARDPGAMDYWGQGTLVTVSS SEQ ID NO.:41 (variant 4 heavy chain le region: th4) QIQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVKQAPGQGLEWIGDINPYNGDTNY NQKFKGKATLTVDKSTSTAYMELSSLRSEDTAVYYCARDPGAMDYWGQGTLVTVSS SEQ ID NO: 42 3A4 murine light (kappa) chain DWMTQTPLSLAVSLGDQASISCRSSQSLLHSNGNTYLEWYLQKPGQSPKLLIHTVSNRFSG VPDRFSGSGSGTDFTLKISRVEAEDLGWYCFQGSHVPLTFGAGTRLELKRTVAAPSVFIFPP SGTASWCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTL SKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC SEQ ID NO:43 3A4 humanized light (kappa) chain variant 1; Lh1 TPLSLPVTPGEPASISCRSSQSLLHSNGNTYLEWYLQKPGQSPQLLIYTVSNRFSGV PDRFSGSGSGTDFTLKISRVEAEDVGWYCFQGSHVPLTFGQGTKLEIKRTVAAPSVFIFPPS DEQLKSGTASWCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLS KADYEKHKVYACEVTHQGLSSPVTKSFNRGEC SEQ ID NO:44 3A4 humanized light (kappa) chain variant 2; Lh2 DWMTQTPLSLPVTPGEPASISCRSSQSLLHSNGNTYLEWYLQKPGQSPKLLIYTVSNRFSG VPDRFSGSGSGTDFTLKISRVEAEDVGWYCFQGSHVPLTFGQGTKLEIKRTVAAPSVFIFPP SDEQLKSGTASWCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTL SKADYEKHKWACEVTHQGLSSPVTKSFNRGEC SEQ ID NO:45 3A4 murine heavy (lgg1) chain QIQLVQSGPEMVKPGASVKMSCKASGYTFTDDYMSWVKQSHGKSLEWIGD!NPYNGDTNY NQKFKGKAILTVDKSSSTAYMQLNSLTSEDSAWYCARDPGAMDYWGQGTSVTVSSASTKG PSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSS WTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPK PKDTLMISRTPEVTCVWDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLT VLHQDWLNGKEYKCKVSNKALPAPiEKTISKAKGQPREPQWTLPPSRDELTKNQVSLTCLV KGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMH EALHNHYTQKSLSLSPGK SEQ ID NO:46 3A4 humanized heavy (Igg1) chain variant 1; HM SGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWMGDINPYNGDTN YNQKFKGRVTITADTSTSTAYMELSSLRSEDTAVYYCARDPGAMDYWGQGTLVTVSSASTK GPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLS SSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPP MISRTPEVTCWVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQWTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVM HEALHNHYTQKSLSLSPGK SEQ ID NO:47 3A4 humanized heavy (Igg1) chain variant 2; Hh2 QlQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWMGDINPYNGDTNY NQKFKGRVTITADKSTSTAYMELSSLRSEDTAVYYCARDPGAMDYWGQGTLVTVSSASTKG PSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSS WTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPK PKDTLMISRTPEVTCWVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVLT VLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLV KGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMH EALHNHYTQKSLSLSPGK SEQ ID NO:48 3A4 humanized heavy (lgg1) chain variant 3; Hh3 QlQLVQSGAEVKKPGASVKVSCKASGYTFTDDYMSWVRQAPGQGLEWIGDINPYNGDTNY NQKFKGRATLTVDKSTSTAYMELSSLRSEDTAWYCARDPGAMDYWGQGTLVTVSSASTK GPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLS SVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPP MISRTPEVTCVWDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQWTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVM HEALHNHYTQKSLSLSPGK SEQ ID NO:49 3A4 humanized heavy (Igg1) chain variant 4: HM SGAEVKKPGASVKVSCKASGYTFTDDYMSWVKQAPGQGLEWIGDINPYNGDTNY NQKFKGKATLTVDKSTSTAYMELSSLRSEDTAWYCARDPGAMDYWGQGTLV‘WSSASTK GPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLS SWTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPP KPKDTLMISRTPEVTCWVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRWSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQWTLPPSRDELTKNQVSLTCL VKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVM HEALHNHYTQKSLSLSPGK SEQ ID NO:50 ATACCCAAGCTTGCCACCATGGAGACAGACACAC SEQ ID NO:51 ATACCCAAGCTTCATTTCCCGGGAGACAGGGAG SEQ ID NO:52 ATACCCAAGCTTGGGCCACCATGAACTTTCTGCTGTCTTGG SEQ ID NO:53 ATACCCAAGCTTCTAACACTCTCCCCTGTTGAAG SEQ ID NO:54 pK-CR5 CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCAT TTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGA TAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCA ACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCC TAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG ATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAA GAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGT AACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTC AGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGC TGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCA GTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC GAATTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCACATCGGCGC GCCAAATGATTTGCCCTCCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACAC ACTATTGCAATGAAAATAAATTTCCTTTATTAGCCAGAGGTCGAGATTTAAATAAGCTTGC TAGCAGATCTTTGGACCTGGGAGTGGACACCTGTGGAGAGAAAGGCAAAGTGGATGTCA TTGTCACTCAAGTGTATGGCCAGATCGGGCCAGGTGAATATCAAATCCTCCTCGTTTTTG GAAACTGACAATCTTAGCGCAGAAGTAATGCCCGCTTTTGAGAGGGAGTACTCACCCCA ACAGCTGGATCTCAAGCCTGCCACACCTCACCTCGACCATCCGCCGTCTCAAGACCGCC TACTTTAATTACATCATCAGCAGCACCTCCGCCAGAAACAACCCCGACCGCCACCCGCT GCCGCCCGCCACGGTGCTCAGCCTACCTTGCGACTGTGACTGGTTAGACGCCTTTCTC GAGAGGTTTTCCGATCCGGTCGATGCGGACTCGCTCAGGTCCCTCGGTGGCGGAGTAC CGTTCGGAGGCCGACGGGTTTCCGATCCAAGAGTACTGGAAAGACCGCGAAGAGTTTG TCCTCAACCGCGAGCCCAACAGCTGGCCCTCGCAGACAGCGATGCGGAAGAGAGTGAC CGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCG TCTCCAGGCGATCTGACGGTTCACTAAACGAGCTCTGCTTATATAGGCCTCCCACCGTA CACGCCTACCTCGACCCGGGTACCAATCTTATAATACAAACAGACCAGATTGTCTGTTTG TTATAATACAAACAGACCAGATTGTCTGTTTGTTATAATACAAACAGACCAGATTGTCTGT TTGTTATAATACAAACAGACCAGATTGTCTGTTTGTTATAATACAAACAGACCAGATTGTC TGTTTGTTATAATACAAACAGACCAGATTGTCTGTTTGTTAAGGTTGTCGAGTGAAGACG AAAGGGTTCATTAAGGCGCGCCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTG TTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGT GTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAA AGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCG AGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGG GAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCT CGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATC CACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCC CGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGA GCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTT ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACG CTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG GTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGA AGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGG TAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCA TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTC TGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAG GATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATG AGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCT GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGG AGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGC TCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCT GCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGT GTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACG CTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACAT GATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGA AGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACT GTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGA GAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGC GCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACT CTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAA ATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTT TTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATG TATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCAC SEQ ID NO:55 pMPG-CR5 GTCGACGATACCGTGCACTTAATTAAGCGCGCTCGACCAAATGATTTGCCCTCCCATATG TCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTGCAATGAAAATAAATTTCCT TTATTAGCCAGAGGTCGAGGTCGGGGGATCCGTTTAAACTTGGACCTGGGAGTGGACAC CTGTGGAGAGAAAGGCAAAGTGGATGTCATTGTCACTCAAGTGTATGGCCAGATCGGGC CAGGTGAATATCAAATCCTCCTCGTTTTTGGAAACTGACAATCTTAGCGCAGAAGTAATG CCCGCTTTTGAGAGGGAGTACTCACCCCAACAGCTGGATCTCAAGCCTGCCACACCTCA CCTCGACCATCCGCCGTCTCAAGACCGCCTACTTTAATTACATCATCAGCAGCACCTCC GCCAGAAACAACCCCGACCGCCACCCGCTGCCGCCCGCCACGGTGCTCAGCCTACCTT GCGACTGTGACTGGTTAGACGCCTTTCTCGAGAGGTTTTCCGATCCGGTCGATGCGGAC TCGCTCAGGTCCCTCGGTGGCGGAGTACCGTTCGGAGGCCGACGGGTTTCCGATCCAA TGGAAAGACCGCGAAGAGTTTGTCCTCAACCGCGAGCCCAACAGCTGGCCCT CAGCGATGCGGAAGAGAGTGACCGCGGAGGCTGGATCGGTCCCGGTGTCTT CTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGGTTCACTAAACG AGCTCTGCTTATATAGGCCTCCCACCGTACACGCCTACCTCGACCCGGGTACCAATCTT ATAATACAAACAGACCAGATTGTCTGTTTGTTATAATACAAACAGACCAGATTGTCTGTTT GTTATAATACAAACAGACCAGATTGTCTGTTTGTTATAATACAAACAGACCAGATTGTCTG TTTGTTATAATACAAACAGACCAGATTGTCTGTTTGTTATAATACAAACAGACCAGATTGT CTGTTTGTTAAGGTTGTCGAGTGAAGACGAAAGGGTTAATTAAGGCGCGCCGTCGACTA GCTTGGCACGCCAGAAATCCGCGCGGTGGTTTTTGGGGGTCGGGGGTGTTTGGCAGCC ACAGACGCCCGGTGTTCGTGTCGCGCCAGTACATGCGGTCCATGCCCAGGCCATCCAA AAACCATGGGTCTGTCTGCTCAGTCCAGTCGTGGACCAGACCCCACGCAACGCCCAAAA TAATAACCCCCACGAACCATAAACCATTCCCCATGGGGGACCCCGTCCCTAACCCACGG TGGCTATGGCAGGGCCTGCCGCCCCGACGTTGGCTGCGAGCCCTGGGCCTT CACCCGAACTTGGGGGGTGGGGTGGGGAAAAGGAAGAAACGCGGGCGTATTGGCCCC AATGGGGTCTCGGTGGGGTATCGACAGAGTGCCAGCCCTGGGACCGAACCCCGCGTTT ATGAACAAACGACCCAACACCCGTGCGTTTTATTCTGTCTTTTTATTGCCGTCATAGCGC GGGTTCCTTCCGGTATTGTCTCCTTCCGTGTTTCAGTTAGCCTCCCCCATCTCCCCTATT CCTTTGCCCTCGGACGAGTGCTGGGGCGTCGGTTTCCACTATCGGCGAGTACTTCTACA CAGCCATCGGTCCAGACGGCCGCGCTTCTGCGGGCGATTTGTGTACGCCCGACAGTCC CGGCTCCGGATCGGACGATTGCGTCGCATCGACCCTGCGCCCAAGCTGCATCATCGAA ATTGCCGTCAACCAAGCTCTGATAGAGTTGGTCAAGACCAATGCGGAGCATATACGCCC GGAGCCGCGGCGATCCTGCAAGCTCCGGATGCCTCCGCTCGAAGTAGCGCGTCTGCTG CTCCATACAAGCCAACCACGGCCTCCAGAAGAAGATGTTGGCGACCTCGTATTGGGAAT CCCCGAACATCGCCTCGCTCCAGTCAATGACCGCTGTTATGCGGCCATTGTCCGTCAGG ACATTGTTGGAGCCGAAATCCGCGTGCACGAGGTGCCGGACTTCGGGGCAGTCCTCGG CCCAAAGCATCAGCTCATCGAGAGCCTGCGCGACGGACGCACTGACGGTGTCGTCCAT CACAGTTTGCCAGTGATACACATGGGGATCAGCAATCGCGCATATGAAATCACGCCATG TAGTGTATTGACCGATTCCTTGCGGTCCGAATGGGCCGAACCCGCTCGTCTGGCTAAGA TCGGCCGCAGCGATCGCATCCATGGCCTCCGCGACCGGCTGCAGAACAGCGGGCAGTT CGGTTTCAGGCAGGTCTTGCAACGTGACACCCTGTGCACGGCGGGAGATGCAATAGGT CAGGCTCTCGCTGAATTCCCCAATGTCAAGCACTTCCGGAATCGGGAGCGCGGCCGAT GCAAAGTGCCGATAAACATAACGATCTTTGTAGAAACCATCGGCGCAGCTATTTACCCGC AGGACATATCCACGCCCTCCTACATCGAAGCTGAAAGCACGAGATTCTTCGCCCTCCGA GAGCTGCATCAGGTCGGAGACGCTGTCGAACTTTTCGATCAGAAACTTCTCGACAGACG TCGCGGTGAGTTCAGGCTTTTTCATATCTCATTGCCCGGGATCTGCGGCACGCTGTTGA TAAGCGGGTCGCTGCAGGGTCGCTCGGTGTTCGAGGCCACACGCGTCACCTT AATATGCGAAGTGGACCTGGGACCGCGCCGCCCCGACTGCATCTGCGTGTTCGAATTC GCCAATGACAAGACGCTGGGCGGGGTTTGTGTCATCATAGAACTAAAGACATGCAAATA TATTTCTTCCGGGGACACCGCCAGCAAACGCGAGCAACGGGCCACGGGGATGAAGCAG GGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGG ATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCA GGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTC GCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGC CGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTT GTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGG AAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCT TGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCC ATCTCCAGCAGCCGCACGCGGCGCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTT GTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAG CTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG GTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCA CTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGC GCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACA AACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA AAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTT AAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAG TTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCC AGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAA CCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATC CAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGC AACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTC ATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAA AGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTAT CACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCT TTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGA GTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAA ATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTG AGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTC ACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAG GGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTAT CAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAG GGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCA TGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTCTCAT GTTTGACAGCTTATCTCTAGCAGATCCGGAATTCCCCTCCCCAATTTAAATGAGGACCTA ACCTGTGGAAATCTACTGATGTGGGAGGCTGTAACTGTACAAACAGAGGTTATTGGAATA ACTAGCATGCTTAACCTTCATGCAGGGTCACAAAAAGTGCATGACGATGGTGGAGGAAA ACCTATTCAAGGCAGTAATTTCCACTTCTTTGCTGTTGGTGGAGACCCCTTGGAAATGCA GGGAGTGCTAATGAATTACAGGACAAAGTACCCAGATGGTACTATAACCCCTAAAAACCC AACAGCCCAGTCCCAGGTAATGAATACTGACCATAAGGCCTATTTGGACAAAAACAATGC TTATCCAGTTGAGTGCTGGGTTCCTGATCCTAGTAGAAATGAAAATACTAGGTATTTTGG GACTTTCACAGGAGGGGAAAATGTTCCCCCAGTACTTCATGTGACCAACACAGCTACCA CAGTGTTGCTAGATGAACAGGGTGTGGGGCCTCTTTGTAAAGCTGATAGCCTGTATGTTT CAGCTGCTGATATTTGTGGCCTGTTTACTAACAGCTCTGGAACACAACAGTGGAGAGGC CTTGCAAGATATTTTAAGATCCGCCTGAGAAAAAGATCTGTAAAGAATCCTTACCTAATTT CCTTTTTGCTAAGTGACCTTATAAACAGGAGAACCCAGAGAGTGGATGGGCAGCCTATG TATGGTATGGAATCCCAGGTAGAAGAGGTTAGGGTGTTTGATGGCACAGAAAGACTTCC AGGGGACCCAGATATGATAAGATATATTGACAAACAGGGACAATTGCAAACCAAAATGCT TTAAACAGGTGCTTTTATTGTACATATACATTTAATAAATGCTGCTTTTGTATAAGCCACTT TTAAGCTTGTGTTATTTTGGGGGTGGTGTTTTAGGCCTTTTAAAACACTGAAAGCCTTTAC ACAAATGCAACTCTTGACTATGGGGGTCTGACCTTTGGGAATGTTCAGCAGGGGCTGAA GAGACTTGGGAAGAGCATTGTGATTGGGATTCAGTGCTTGATCCATGTCCAGA GTCTTCAGTTTCTGAATCCTCTTCTCTTGTAATATCAAGAATACATTTCCCCATGCATATAT TATATTTCATCCTTGAAAAAGTATACATACTTATCTCAGAATCCAGCCTTTCCTTCCATTCA ACAATTCTAGAAGTTAAAACTGGGGTAGATGCTATTACAGAGGTAGAATGCTTCCTAAAC CCAGAAATGGGGGATCTGC SEQ ID NO.:56— 3A4 humanized heavy chain CDR2 polypeptide sequence DINPYNGDTNYNQKFKG

Claims (40)

1. An antibody or antigen binding fragment thereof capable of specific g to Kidney associated antigen 1 (KAAG1) having a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO.:5, SEQ ID NO.:6 and SEQ ID NO.:7 and a light chain variable region comprising the amino acid sequence set forth in SEQ ID NO.: 8, SEQ ID NO.:9 and SEQ ID NO.:10.

2. The antibody or antigen g fragment thereof of claim 1, wherein the heavy chain le region is as set forth in SEQ ID NO.: 35, SEQ ID NO.:36, or SEQ ID NO.:37 and wherein the light chain variable region is as set forth in SEQ ID NO.:30, SEQ ID NO.:31, or SEQ ID NO.:32.

3. The antibody or antigen binding fragment thereof of claim 1, wherein the heavy chain variable region is as set forth in SEQ ID NO.:38, SEQ ID NO.:39, SEQ ID NO.:40, SEQ ID NO.:41 or SEQ ID NO.:2 and wherein the light chain variable region is as set forth in SEQ ID NO.:33, SEQ ID NO.:34 or SEQ ID NO.:4.

4. The antibody or antigen g fragment thereof of any one of claims 1 to 3, wherein the dy comprises the heavy chain set forth in SEQ ID NO.: 49, SEQ ID NO.:46, SEQ ID NO.:47, SEQ ID NO.:48 or SEQ ID NO.:45 and/or the light chain set forth in SEQ ID NO.: 43, SEQ ID NO.:44 or SEQ ID NO.:

5. The antibody or antigen g fragment thereof of claim 3, n said antibody or antigen binding fragment thereof comprises: a. the heavy chain variable region as set forth in SEQ ID NO.:41 and the light chain variable region as set forth in SEQ ID NO.:33; b. the heavy chain as set forth in SEQ ID NO.:49 and the light chain as set forth in SEQ ID NO.:43; c. the heavy chain variable region as set forth in SEQ ID NO.:38 and the light chain le region as set forth in SEQ ID NO.:33; d. the heavy chain as set forth in SEQ ID NO.:46 and the light chain as set forth in SEQ ID NO.:43; e. the heavy chain variable region as set forth in SEQ ID NO.:39 and the light chain variable region as set forth in SEQ ID NO.:33; f. the heavy chain as set forth in SEQ ID NO.:47 and the light chain as set forth in SEQ ID NO.:43; g. the heavy chain variable region as set forth in SEQ ID NO.:40 and the light chain variable region as set forth in SEQ ID NO.:33; h. the heavy chain as set forth in SEQ ID NO.:48 and the light chain as set forth in SEQ ID NO.:43; i. the heavy chain variable region as set forth in SEQ ID NO.:41 and the light chain variable region as set forth in SEQ ID NO.:34; j. the heavy chain as set forth in SEQ ID NO.:49 and the light chain as set forth in SEQ ID ; k. the heavy chain variable region as set forth in SEQ ID NO.:38 and the light chain variable region as set forth in SEQ ID NO.:34; l. the heavy chain as set forth in SEQ ID NO.:46 and the light chain as set forth in SEQ ID NO.:44; m. the heavy chain variable region as set forth in SEQ ID NO.:39 and the light chain variable region as set forth in SEQ ID NO.:34; n. the heavy chain as set forth in SEQ ID NO.:47 and the light chain as set forth in SEQ ID NO.:44; o. the heavy chain variable region as set forth in SEQ ID NO.:40 and the light chain variable region as set forth in SEQ ID NO.:34; p. the heavy chain as set forth in SEQ ID NO.:48 and the light chain as set forth in SEQ ID NO.:44; q. the heavy chain variable region set forth in SEQ ID NO.:2 and the light chain variable region set forth in SEQ ID NO.:4; or r. the heavy chain set forth in SEQ ID NO.:45 and the light chain set forth in SEQ ID NO.:42.

6. An antibody or antigen binding fragment thereof that specifically binds to kidney associated antigen 1 (KAAG1) and has an affinity constant (KD) of less than 10 picomolar.

7. The antibody or antigen binding fragment thereof of claim 6, wherein said antibody or antigen binding fragment thereof competes with the dy or antigen binding fragment thereof of claim 1 to 5.

8. The antibody or antigen g fragment thereof of any one of claims 1 to 3, 6 or 7, wherein the antibody comprises a constant region of a human IgG1 antibody.

9. An antibody or antigen binding fragment thereof capable of competing with the antibody or n binding fragment thereof of any one of claims 1 to 5 and having an affinity constant (KD) of less than 1nM, provided that said antibody is not the 3G10 dy or antigen binding fragment thereof.

10. The dy or antigen binding fragment thereof of claim 1, having a light chain variable region at least 70% identical to SEQ ID NO.:4 and/or a heavy chain variable region at least 70% identical to SEQ ID NO.:2 and sing at least one amino acid substitution e of a complementarity determining region (CDR) in comparison with SEQ ID NO.:4 or SEQ ID NO.:2..

11. The antibody or antigen g fragment thereof of any one of claims 1 to 3 or 6 to 10, wherein the antibody is a monoclonal antibody, a chimeric dy, an hybrid antibody, a humanized antibody or a human antibody or an antigen binding fragment thereof.

12. The antibody or antigen binding fragment of any one of claims 1 to 11, wherein the antibody or antigen binding fragment thereof is conjugated with a therapeutic moiety or with a detectable moiety.

13. The antibody or antigen binding fragment of any one of claims 1 to 11, wherein the antibody or antigen binding fragment thereof is conjugated with a cytotoxic agent.

14. The antibody or antigen binding fragment thereof of claim 13, n the cytotoxic agent comprises an auristatin.

15. The antibody or antigen binding fragment thereof of claim 14, wherein the auristatin comprises monomethyl auristatin E or monomethyl auristatin F.

16. A nucleic acid encoding a light chain le region and/or a heavy chain variable region of the antibody or antigen g fragment of any one of claims 1 to 11.

17. A vector comprising the c acid of claim 16.

18. The vector of claim 17, wherein said vector is an expression vector.

19. An isolated cell comprising the nucleic acid of claim 16, the vector of claim 17 or the antibody or antigen binding fragment of any one of claims 1 to 15.

20. The isolated cell of claim 19, n said cell comprises a nucleic acid encoding a light chain variable region and a nucleic acid ng a heavy chain variable region.

21. The isolated cell of claim 20, wherein said cell is capable of expressing, ling and/or secreting an antibody or antigen binding fragment thereof.

22. A pharmaceutical composition comprising the antibody or antigen binding fragment of any one of claims 1 to 15, and a pharmaceutically acceptable

23. A composition comprising the antibody or antigen binding fragment of any one of claims 1 to 15, and a carrier.

24. A method for detecting KAAG1 or a KAAG1 variant, the method comprising contacting an isolated cell expressing KAAG1 or the KAAG1 variant or a sample comprising or suspected of comprising KAAG1 or the KAAG1 variant with the antibody or antigen binding fragment thereof of any one of claims 1 to 15 and measuring binding.

25. The method of claim 24, wherein the sample is from a subject having or suspected of having cancer.

26. The method of claim 25, wherein the cancer is metastatic.

27. The method of any one of claims 24 to 26, wherein the sample is a serum , a plasma sample or a blood sample obtained from a mammal.

28. The method of any one of claims 24 to 26, n the sample is a tissue sample obtained from a mammal.

29. The method of any one of claims 24 to 26, wherein the sample is a cell e or a supernatant.

30. The method of any one of claims 24 to 29, comprising quantifying the amount of antibody bound to KAAG1 or the KAAG1 variant.

31. A kit comprising the antibody or antigen binding nt of any one of claims 1 to 15.

32. Use of the dy or antigen binding fragment of any one of claims 1 to 15, the pharmaceutical composition of claim 22 or the composition of claim 23 in the manufacture of a medicament for detection, diagnosis or treatment of cancer comprising cells expressing KAAG1 or a KAAG1 variant.

33. Use of the antibody or antigen g fragment of any one of claims 1 to 15, the ceutical composition of claim 22 or the composition of claim 23 in the detection of a tumor ex vivo or in the diagnosis of cancer ex vivo, wherein the tumor or cancer comprises cells expressing KAAG1 or a KAAG1 variant.

34. The use as d in claim 32 or claim 33, wherein the cancer is selected from the group consisting of ovarian cancer, skin cancer, renal cancer, colorectal , sarcoma, leukemia, brain cancer, d cancer, breast cancer, prostate cancer, oesophageal cancer, bladder cancer, lung cancer and head and neck cancer.

35. The use as defined in claim 34, wherein the cancer is ovarian cancer.

36. The use as defined in claim 35, wherein the cancer is recurrent n cancer.

37. The use as defined in any one of claims 32 to 36, wherein the cancer is metastatic.

38. A method for obtaining an antibody or antigen binding fragment thereof, le for use as an antibody-drug conjugate for the treatment of cancer sing cells expressing KAAG1 or a KAAG1 variant, the method comprising: a. providing an antibody or antigen binding fragment thereof which specifically binds to an epitope comprised between amino acids 61 and 84 of KAAG1 or a KAAG1 variant; b. testing internalization of the antibody or n binding fragment within an isolated cell expressing KAAG1 or a KAAG1 variant or in a non-human animal and; c. isolating an antibody or an antigen binding fragment which is internalized.

39. A method of making the antibody or antigen binding fragment thereof of any one of claims 1 to 11, comprising culturing an isolated cell as defined in any one of claims 19 to 21 so that the antibody or antigen binding fragment thereof is produced.

40. The method of claim 39, comprising conjugating the antibody or n binding nt f with a therapeutic moiety or with a detectable moiety. ‘vn an1