NZ625887B2

NZ625887B2 - Compositions comprising c5 and c6 monosaccharides

Info

Publication number: NZ625887B2
Application number: NZ625887A
Authority: NZ
Inventors: Daniel Clay Floyd; Kiran Kadam; Srinivas Kilambi
Original assignee: Renmatix Inc
Priority date: 2011-12-30
Filing date: 2012-12-03
Publication date: 2016-08-02

Abstract

Disclosed are compositions comprising C6 monosaccharides of varying degrees of polymerization and low levels of undesirable impurities, such as compounds containing aluminium, calcium, iron and sulfur. The compositions are processed from lignocellulosic biomass using supercritical, subcritical, and/or near critical fluid extraction. or near critical fluid extraction.

Description

COMPOSITIONS COMPRISING C5 AND C6 MONOSACCHARIDES CROSS REFERENCE TO RELATED APPLICATIONS This application claims the benefit of: U.S. Application No. 61/581,922 filed December 30, 2011; U.S. Application No. 61/581,907 filed er 30, 2011; U.S. Application No. 61/581,878 filed December 30, 2011; U.S. Application No. 61/581,890 filed December 30, 2011; U.S. Application No.13/649,437 filed October 11, 2012; U.S. Application No. 13/649,343 filed October 11, 2012; U.S. Application No. 13/649,294 filed October 11, 2012; and U.S. Application No.13/649,395 filed October 11, 2012; the entire disclosures of which are orated herein by reference.

FIELD OF THE INVENTION The present invention generally relates to itions comprising C5 and C6 monosaccharides containing maximum levels of undesirable impurities, such as compounds containing sulfur, nitrogen, or metals, ally those processed from lignocellulosic biomass using supercritical, subcritical, and/or near al fluid extraction.

OUND OF THE INVENTION There are a number of processes for converting lignocellulosic biomass into liquid s of various fermentable . Certain red processes are based on supercritical water (SCW) or hot compressed water (HCW) technology, which offer several advantages ing high throughputs, use of mixed feedstocks, separation of sugars, and avoidance of concentrated acids, microbial cultures, and enzymes. Processes using hot compressed water may have two distinct operations: pre-treatment and cellulose hydrolysis. The pre-treatment process hydrolyzes the hemicellulose component of the ellulosic biomass and cellulose hydrolysis (CH) process, as its name infers, hydrolyzes the cellulose fibers. The resultant five 2012/067538 carbon (C5) and six carbon (C6) sugar streams are recovered separately. The remaining solids, which consist mostly of lignin, are preferably red, such as through filtration, and may be used as a fuel to provide thermal energy to the process itself or for other processes.

Among their many uses, the sugar streams may be converted to ethanol through fermentation using yeast or bacteria that feed on the sugars. As the sugars are consumed, ethanol and carbon dioxide are produced.

The invention is directed to these compositions, as well as and other important ends.

SUMMARY OF THE INVENTION In a first embodiment, the invention is directed to compositions, comprising C6 monosaccharides. In particular, the compositions comprise: at least one soluble C6 monosaccharide hydrolysate; and less than about 6750 ppm in total by weight, based on total weight of water- e C6 saccharide hydrolysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In n embodiments, the compositions comprise: at least one water-soluble C6 monosaccharide hydrolysate; less than about 10 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of aluminum; less than about 350 ppm by weight, based on total weight of water-soluble C6 ride hydrolysate in said ition, of calcium; less than about 425 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of iron; and less than about 4500 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of sulfur.

In certain ments, the compositions comprise: at least one water-soluble C6 monosaccharide hydrolysate; and less than about 10 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of um.

In certain embodiments, the compositions se: at least one water-soluble C6 monosaccharide hydrolysate; and less than about 350 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said ition, of calcium.

In certain embodiments, the compositions comprise: at least one water-soluble C6 monosaccharide hydrolysate; and less than about 425 ppm by , based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of iron.

In certain embodiments, the compositions comprise: at least one water-soluble C6 monosaccharide hydrolysate; and less than about 4500 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of sulfur.

In other embodiments, the invention is directed to compositions, comprising C5 monosaccharides. In particular, the compositions comprise: at least one water-soluble C5 ccharide hydrolysate; and less than about 1950 ppm in total by weight, based on total weight of water- e C5 saccharide hydrolysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In certain embodiments, the compositions comprise: at least one water-soluble C5 monosaccharide ysate; less than about 5 ppm by weight, based on total weight of water-soluble C5 ride hydrolysate in said composition, of aluminum; less than about 300 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of calcium; less than about 10 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of iron; and less than about 1000 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of sulfur.

In certain embodiments, the compositions comprise: at least one water-soluble C5 monosaccharide ysate; and less than about 5 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of aluminum.

In certain embodiments, the compositions comprise: at least one water-soluble C5 monosaccharide hydrolysate; and less than about 300 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of calcium.

In certain embodiments, the compositions comprise: at least one water-soluble C5 monosaccharide hydrolysate; and less than about 10 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of iron.

In certain embodiments, the compositions comprise: at least one water-soluble C5 ccharide hydrolysate; and less than about 1000 ppm by weight, based on total weight of water-soluble C5 saccharide ysate in said composition, of sulfur.

In certain embodiments, the ion is directed to methods of reducing the level of enzyme required for enzymatically hydrolyzing first soluble C6 saccharides having an average degree of polymerization to about 2 to about 15, preferably about 2 to about 10, and more preferably about 2 to about 6, to second water-soluble C6 saccharides having a lower average degree of rization than said average degree of rization of said first water-soluble C6 saccharides, comprising: providing a hydrolysate sing said first water-soluble C6 saccharides and less than about 5250 ppm in total by weight, based on total weight of water- soluble C6 saccharide hydrolysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In certain embodiments, the invention is directed to methods of reducing the level of enzyme required for enzymatically hydrolyzing first water-soluble C5 saccharides having an average degree of polymerization to about 2 to about 28, preferably about 2 to about 15, more ably about 2 to about 13, even more preferably about 2 to about 6, to second water-soluble C5 saccharides having a lower average degree of rization than said average degree of polymerization of said first water-soluble C5 saccharides, comprising: providing a hydrolysate comprising said first water-soluble C5 saccharides and less than about 3700 ppm in total by weight, based on total weight of water- soluble C5 saccharide hydrolysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

DETAILED DESCRIPTION OF THE INVENTION As employed above and throughout the disclosure, the following terms, unless othenNise indicated, shall be understood to have the following gs.

As used herein, the singular forms “a, an,” and “the” include the plural reference unless the context clearly indicates otherwise.

While the present invention is capable of being embodied in various forms, the description below of several ments is made with the understanding that the present disclosure is to be considered as an exemplification of the invention, and is not ed to limit the invention to the specific embodiments illustrated.

Headings are provided for convenience only and are not to be construed to limit the invention in any . Embodiments illustrated under any heading may be combined with ments illustrated under any other heading.

The use of numerical values in the various quantitative values specified in this application, unless expressly indicated otherwise, are stated as approximations as though the minimum and maximum values within the stated ranges were both preceded by the word “about.” In this , slight variations from a stated value can be used to achieve substantially the same results as the stated value. Also, the disclosure of ranges is intended as a continuous range including every value between the minimum and m values recited as well as any ranges that can be formed by such values. Also sed herein are any and all ratios (and ranges of any such ratios) that can be formed by dividing a recited numeric value into any other recited c value. Accordingly, the skilled person will appreciate that many such ratios, ranges, and ranges of ratios can be unambiguously derived from the numerical values presented herein and in all instances such ratios, ranges, and ranges of ratios represent s embodiments of the present invention.

As used herein, the phrase “substantially free” means have no more than about 1%, preferably less than about 0.5%, more preferably, less than about 0.1%, by weight of a component, based on the total weight of any composition containing the component.

A supercritical fluid is a fluid at a temperature above its critical temperature and at a pressure above its critical pressure. A supercritical fluid exists at or above its “critical point,” the point of highest temperature and pressure at which the liquid and vapor (gas) phases can exist in equilibrium with one another. Above critical pressure and critical temperature, the distinction between liquid and gas phases disappears. A supercritical fluid possesses approximately the penetration properties of a gas simultaneously with the solvent properties of a liquid. Accordingly, supercritical fluid tion has the benefit of high penetrability and good solvation.

Reported critical temperatures and pressures include: for pure water, a critical temperature of about 374.2°C, and a critical pressure of about 221 bar; for carbon dioxide, a critical ature of about 31°C and a al pressure of about 72.9 atmospheres (about 1072 psig). Near critical water has a temperature at or above about 300°C and below the critical temperature of water (374.2°C), and a pressure high enough to ensure that all fluid is in the liquid phase. Sub-critical water has a temperature of less than about 300°C and a pressure high enough to ensure that all fluid is in the liquid phase. Sub-critical water temperature may be greater than about 250°C and less than about 300°C, and in many instances sub-critical water has a temperature between about 250°C and about 280°C. The term “hot ssed water” is used interchangeably herein for water that is at or above its critical state, or defined herein as near-critical or sub-critical, or any other temperature above about 50°C (preferably, at least about 100°C) but less than subcritical and at pressures such that water is in a liquid state As used herein, a fluid which is “supercritical” (e.g. supercritical water, supercritical C02, etc.) indicates a fluid which would be supercritical if present in pure form under a given set of temperature and pressure conditions. For example, “supercritical water” indicates water present at a temperature of at least about 374.2°C and a pressure of at least about 221 bar, r the water is pure water, or present as a mixture (e.g. water and ethanol, water and C02, etc). Thus, for example, “a mixture of sub-critical water and supercritical carbon dioxide” indicates a mixture of water and carbon dioxide at a temperature and pressure above that of the critical point for carbon dioxide but below the critical point for water, regardless of r the supercritical phase contains water and regardless of whether the water phase contains any carbon dioxide. For example, a mixture of itical water and supercritical C02 may have a temperature of about 250°C to about 280°C and a pressure of at least about 225 bar.

As used herein, “lignocellulosic biomass or a component part f’ refers to plant biomass containing cellulose, hemicellulose, and lignin from a variety of sources, including, without limitation (1) agricultural residues (including corn stover and sugarcane e), (2) dedicated energy crops, (3) wood residues (including hardwoods, softwoods, sawmill and paper mill discards), and (4) municipal waste, and their constituent parts including without limitation, ellulose biomass itself, lignin, C5 saccharides (including cellulose, cellobiose, C5 oligosaccharides, C5 ccharides, C5 saccharides (including hemicellulose, C5 oligosaccharides, and C5 monosaccharides), and mixtures thereof.

As used herein, “ash” refers to the non-aqueous residue that remains after a sample is burned, and consists mostly of metal oxides. Ash content may be measured in accordance with ASTM Standard Method No. 01 “Standard Method for the Determination of Ash in Biomass.” This test method covers the determination of ash, sed as the percentage of residue remaining after dry oxidation at 550 to 600°C. All results are reported relative to the 105°C oven dry weight of the sample. See also: http://www.nrel.gov/biomass/pdfs/42622.pdf and http://www.astm.org/Standards/ E1755.htm, which are both incorporated herein by reference in their entirety.

As used herein, “degree of polymerization” refers to the number of monomeric units in a macromolecule or polymer or oligomer molecule, including those monomeric units that are not identical (such as in a oligomer with different monomeric residues). The degree of polymerization (DP) of the various saccharides in the compositions of the invention may be measured using gel permeation chromatography (GPC), high pressure liquid tography (HPLC), such as DIONEX with an electrochemical detector, -assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, or other conventional lar weight determination methods.

C6 ccharides Accordingly, in one embodiment, the invention is directed to compositions, comprising C6 monosaccharides. In particular embodiments, the compositions comprise: at least one water-soluble C6 ccharide hydrolysate; and less than about 6750 ppm in total by weight, based on total weight of water- soluble C6 saccharide ysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In certain red embodiments, said elements are present at a level of less than about 6600 ppm in total by weight, based on total weight of water-soluble C6 ride hydrolysate in said composition, of said elements.

In certain embodiments, the compositions comprise: at least one water-soluble C6 monosaccharide hydrolysate; less than about 10 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of aluminum; less than about 350 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said ition, of calcium; less than about 425 mg, based on total weight of water-soluble C6 saccharide ysate in said composition, of iron; and less than about 4500 ppm by weight, based on total weight of water-soluble C6 ride hydrolysate in said composition, of sulfur.

In certain embodiments, the compositions comprise: at least one water-soluble C6 monosaccharide hydrolysate; and less than about 10 ppm by , based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of um.

In certain embodiments, the compositions comprise: at least one water-soluble C6 monosaccharide hydrolysate; and less than about 350 ppm by , based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of calcium.

In certain embodiments, the compositions se: at least one water-soluble C6 ccharide hydrolysate; and less than about 425 mg, based on total weight of water-soluble C6 saccharide hydrolysate in said ition, of iron.

In certain preferred embodiments of the compositions comprising the water- soluble C6 monosaccharide hydrolysate, the compositions comprise: less than about 6750 ppm in total by weight, based on total weight of water- soluble C6 saccharide hydrolysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In certain red embodiments, said elements are t at a level of less than about 6600 ppm in total by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of said elements.

In certain embodiments, the water-soluble C6 monosaccharide hydrolysate is ted from lignocellulosic biomass. In certain ments, the soluble C6 monosaccharide hydrolysate is processed from lignocellulosic biomass using ritical, subcritical, or near critical fluid extraction, or a combination thereof.

In n embodiments, the itions further comprise water.

In certain embodiments, the water-soluble C6 monosaccharide hydrolysate is present at a concentration of at least about 0.5 g/L.

In certain embodiments, the C6 monosaccharide is glucose, galactose, mannose, fructose, or a mixture thereof.

In certain preferred embodiments of the compositions comprising the water- soluble C6 monosaccharide hydrolysate, the compositions further comprise less than about 10 ppm by , based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of aluminum, preferably less than about 7.5 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of aluminum.

In certain preferred embodiments of the compositions comprising the water- soluble C6 monosaccharide hydrolysate, the compositions further comprise less than about 350 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of calcium, preferably less than about 325 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said ition, of calcium.

In certain preferred embodiments of the compositions comprising the water- soluble C6 monosaccharide hydrolysate, the compositions further comprise less than about 425 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of iron, preferably less than about 420 ppm by , based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of iron.

In certain preferred embodiments of the compositions comprising the water- soluble C6 monosaccharide hydrolysate, the itions r comprise less than about 4500 ppm by weight, based on total weight of water-soluble C6 saccharide ysate in said composition, of sulfur, preferably less than about 4425 ppm by weight, based on total weight of water-soluble C6 saccharide hydrolysate in said composition, of sulfur.

In certain preferred embodiments of the compositions comprising the water- soluble C6 monosaccharide hydrolysate, the weight ratio of said water-soluble C6 monosaccharide hydrolysate to said elements is greater than about 25:1, preferably greater than about 30:1.

In n red embodiments of the compositions comprising the water- soluble C6 monosaccharide hydrolysate, the compositions r comprise at least one water-soluble C6 oligosaccharide having a degree of polymerization of about 2 to about 15, preferably about 2 to about 13, more preferably about 2 to about 10, and even more preferably about 2 to 6.

In certain preferred embodiments of the compositions comprising the water- soluble C6 ccharide ysate, the compositions further comprise at least one water-soluble C5 saccharide having a degree of polymerization of about 1 to about 28, preferably about 1 to 15, more preferably about 1 to about 10, and even more preferably about 1 to about 6.

In n embodiments, the water-soluble C6 monosaccharide hydrolysate is processed from Iignocellulosic biomass using supercritical, subcritical, or near critical fluid extraction, or a combination thereof.

In certain embodiments, the level of said elements are measured by ively coupled plasma emission spectroscopy.

In other embodiments, the compositions comprise less than about 1500 mg of nitrogen per kg of total weight of water-soluble C6 saccharides, preferably less than about 1450 mg of nitrogen per kg of total weight of water-soluble C6 rides. en may be measured by thermal conductivity ion after combustion and reduction.

In yet other embodiments, the compositions further comprise a weight ratio of the total mass of hydrogen and nitrogen to carbon of less than about 0.17.

In certain other embodiments, the compositions comprising the C6 monosaccharides further comprise less than a maximum of any of the ts, individually or in combination, in the table listed below: Level less than about (ppm or mg of element/kg of C6 saccharides Level less than about (ppm or mg of element/kg of C6 saccharides C5 monosaccharides Accordingly, in one embodiment, the invention is directed to compositions, sing C5 monosaccharides. In particular, the compositions comprise: at least one water-soluble C5 monosaccharide hydrolysate; and less than about 1950 ppm in total by weight, based on total weight of water- soluble C5 saccharide hydrolysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In certain red embodiments, the elements are t at a level of less than about 1925 ppm in total by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition.

In certain embodiments, the compositions comprise: at least one water-soluble C5 monosaccharide hydrolysate; less than about 5 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of aluminum; less than about 300 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said ition, of calcium; less than about 10 mg based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of iron; and less than about 1000 ppm by weight, based on total weight of soluble C5 saccharide hydrolysate in said composition, of sulfur.

In certain embodiments, the itions comprise: at least one water-soluble C5 monosaccharide hydrolysate; and less than about 5 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of aluminum.

In n embodiments, the compositions comprise: at least one water-soluble C5 monosaccharide hydrolysate; and less than about 10 mg, based on total weight of water-soluble C5 saccharide ysate in said composition, of iron.

In certain embodiments, the compositions comprise: at least one water-soluble C5 monosaccharide hydrolysate; and less than about 1000 ppm by weight, based on total weight of water-soluble C5 saccharide ysate in said composition, of sulfur.

In certain embodiments, the compositions further comprise: less than about 1950 ppm in total by weight, based on total weight of water- soluble C5 saccharide hydrolysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In certain preferred embodiments, the elements are t at a level of less than about 1925 ppm in total by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said ition.

In certain preferred embodiments of the compositions comprising the water- soluble C5 monosaccharide hydrolysate, the compositions further comprise less than about 5 ppm by weight, based on total weight of soluble C5 saccharide ysate in said composition, of aluminum, preferably less than about 2 ppm by , based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of aluminum.

In certain red embodiments of the compositions comprising the water- soluble C5 monosaccharide hydrolysate, the compositions r comprise less than about 300 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of calcium, preferably less than about 280 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of calcium.

In certain preferred ments of the compositions comprising the water- soluble C5 monosaccharide hydrolysate, the compositions further comprise less than about 10 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of iron, preferably less than about 5 ppm by , based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of iron.

In certain preferred embodiments of the compositions comprising the water- soluble C5 monosaccharide hydrolysate, the compositions r comprise less than about 1000 ppm by weight, based on total weight of water-soluble C5 saccharide hydrolysate in said composition, of sulfur, preferably less than about 975 ppm by weight, based on total weight of soluble C5 ride hydrolysate in said composition, of sulfur.

In certain preferred embodiments, the weight ratio of said C5 monosaccharide to said elements is greater than about 90:1, preferably greater than about 95:1.

In certain embodiments, the compositions further comprise water.

In certain embodiments, the water-soluble C5 monosaccharide hydrolysate is present at a concentration of at least 0.5 g/L.

In certain embodiments, the C5 monosaccharide is xylose, arabinose, lyxose, ribose, or a mixture thereof.

In certain preferred embodiments of the compositions comprising the water- soluble C5 monosaccharide hydrolysate, the compositions r comprise at least one water-soluble C5 oligosaccharide having a degree of polymerization of about 2 to about 28, preferably about 2 to about 15, more preferably about 2 to about 10, and even more preferably about 2 to 6.

In certain red embodiments of the compositions comprising the water- soluble C5 monosaccharide hydrolysate, the compositions further comprise at least one water-soluble C6 saccharide having a degree of polymerization of about 1 to about 15, preferably about 1 to 13, more preferably about 1 to about 10, and even more ably about 1 to about 6.

In n preferred embodiments of the compositions comprising the water- soluble C5 monosaccharide hydrolysate, the itions r comprise at least one water-soluble C6 saccharide having a degree of polymerization of about 1 to about 15, preferably about 1 to about 13, more preferably about 1 to about 10, and even more preferably about 1 to 6.

In certain embodiments, the water-soluble C5 ccharide hydrolysate is processed from lignocellulosic biomass using supercritical, subcritical, or near critical fluid extraction, or a combination thereof.

In certain ments, the level of said elements are measured by inductively coupled plasma emission spectroscopy.

In other embodiments, the compositions comprise less than about 350 mg of nitrogen per kg of total weight of soluble C5 rides, preferably less than about 325 mg of nitrogen per kg of total weight of water-soluble C5 saccharides. Nitrogen may be measured by thermal conductivity detection after combustion and reduction. 2012/067538 In yet other embodiments, the itions further comprise a weight ratio of the total mass of hydrogen and nitrogen to carbon of less than about 0.17.

Carbon, en, and nitrogen levels may be measured by thermal conductivity detection after combustion and reduction.

In certain other embodiments, the compositions comprising the C5 monosaccharides further comprise less than a maximum of any of the elements, individually or in combination, in the table listed below: mLevel less than about (ppm or mg of element/kg of C5 saccharides -§- 1.0 -§_ 0.25 -§_ 0.1 0-25 0.25 -_ 0.15 -i_ 0.25 0.20 0.25 0-25 1.25 0-25 Further ments In further embodiments, the compositions further comprise less than about 0.5% by weight, based on the total weight of said C5 ccharides or C6 monosaccharides, of organic solvent, such as alcohols, including water miscible lower aliphatic C1-C4 alcohols (e.g., methanol, ethanol, isopropanol, t—butanol). In preferred embodiments, the compositions contain less than about 0.1% by weight, based on the total weight of said of said C5 monosaccharides or C6 monosaccharides of organic solvent. In more preferred embodiments, the itions contain substantially no organic solvent.

The compositions of the invention are preferably prepared from biomass by processes employing supercritical, subcritical, and/or near critical water, preferably without the addition of acid. The processes may include pretreatment step or steps using ritical or near critical water to separate the C5 sugars (monomers and/or oligomers) from cellulose and lignin. In the pretreatment step, suitable temperatures are about 130°C to about 250°C, suitable pressures are about 4 bars to about 100 bars, and suitable residence times are about 0.5 minutes to about 5 hours. The processes may also include a cellulose ysis step or steps using supercritical or near critical water to separate the ose (which may processed to form C6 ric and/or oligomeric sugars) from the lignin. In the hydrolysis step(s), suitable atures are about 250°C to about 450°C, suitable pressures are about 40 bars to about 260 bars, and suitable residence times are about 0.1 seconds to about 3 minutes. The C5 monomers in the pretreatment step are formed by autohydrolysis both from xylan ly and/or from C5 oligomers. Similarly, the C6 monomers are formed in the supercritical hydrolysis step directly from the cellulose and/or from C6 oligomers. The pretreatment step also produces small amounts of C6 sugars in oligomeric and/or monomeric form. The C5 and C6 oligomers may be hydrolyzed to their respective monomers using acid, such as sulfuric acid. The compositions may be prepared in any suitable reactor, including, but not limited to, a tubular reactor, a digester (vertical, horizontal, or ed), or the like. Suitable digesters include the digester system described in US—B-8,057,639, which include a er and a steam explosion unit, the entire disclosure of which is incorporated by reference.

The compositions of the invention comprising C5 monosaccharides or C6 monosaccharides may be utilized in a wide variety of applications, where C5 and C6 sugars are conventionally utilized, including, but not limited to, the production of various chemicals and fuels using fermentative, enzymatic, catalytic, and non- catalytic (e.g., thermal decomposition) processes. Such processes are useful for preparing feedstocks for the ation of the following non-exhaustive list: fuels (such as ne, jet fuel, butanol, and the like); chemicals (such as acetic acid, acetic anhydride, acetone, acrylic acid, adipic acid, benzene, ethanol, ethylene, ethylene , ethylene oxide, methanol, opylene, thalic acid, toluene, xylene, 1,3-propanediol, 1,4-butanediol, and the like); pharmaceuticals and foods (such as acetoin, e, arabitol, ascorbic acid, ic acid, citric acid, coumaric acid, fumaric acid, glycerol, glycine, kojic acid, lactic acid, , malonic acid, proline, propionic acid, serine, sorbitol, succinic acid, threonine, l, sugar acids (glucaric acid, gluconic acid, xylonic acids), and the like); specialty chemicals (such as acontic acid, glutamic acid, malic acid, oxalic acid, and the like); textile applications (such as formic acid and the like); and industrial intermediates (acetaldehyde, oxypropionic acid, 2,5-furan dicarboxylic acid, furfural, glutaric acid, itaconic acid, levulinic acid, and the like).

In certain embodiments, the invention is directed to methods of reducing the level of enzyme required for enzymatically hydrolyzing first water-soluble C6 saccharides having an average degree of polymerization to about 2 to about 15, preferably about 2 to about 10, and more preferably about 2 to about 6, to second water-soluble C6 saccharides having a lower average degree of polymerization than said average degree of rization of said first water-soluble C6 saccharides, comprising: providing a hydrolysate comprising said first water-soluble C6 saccharides and less than about 5250 ppm in total by weight, based on total weight of water- e C6 saccharide hydrolysate in said composition, of elements; 2012/067538 wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In n embodiments, the C6 saccharides are ted from Iignocellulosic biomass. In other embodiments, the C6 saccharides are processed from Iignocellulosic biomass using supercritical, subcritical, or near critical fluid extraction, or a combination thereof.

In certain embodiments, the invention is directed to methods of reducing the level of enzyme required for tically yzing first water-soluble C5 saccharides having an average degree of polymerization to about 2 to about 28, preferably about 2 to about 15, more preferably about 2 to about 13, even more preferably about 2 to about 6, to second water-soluble C5 saccharides having a lower average degree of rization than said average degree of polymerization of said first water-soluble C5 rides, comprising: providing a hydrolysate comprising said first water-soluble C5 saccharides and less than about 3700 ppm in total by weight, based on total weight of water- soluble C5 saccharide hydrolysate in said composition, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

In certain ments, the C5 saccharides are extracted from Iignocellulosic biomass. In other embodiments, the C5 saccharides are processed from Iignocellulosic biomass using supercritical, subcritical, or near critical fluid extraction, or a combination thereof.

The present invention is further defined in the following Examples, in which all parts and percentages are by weight, unless otherwise stated. It should be understood that these examples, while indicating preferred embodiments of the invention, are given by way of illustration only and are not to be construed as limiting in any manner. From the above sion and these examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various s and modifications of the invention to adapt it to various usages and conditions. 2012/067538 e 1: Preparation of monosaccharide compositions The C5 ccharide and C6 monosaccharide compositions of the invention were prepared using supercritical, tical, and near critical water extraction in a two stage process followed by acid hydrolysis. Particulate lignocellulosic biomass consisting of mixed hardwood chips of 140 mesh or less was mixed with water to form a slurry (about 20% by weight solids). The slurry was heated to a temperature of about 170-245°C and then feed into a pretreatment reactor for about 1-120 minutes under sufficient pressure to keep the water in the liquid phase. The pretreated slurry was then cooled to a temperature less than about 100°C under little (less than about 10 bar) or no pressure. The pretreated solids were then separated from the liquid stream using a filter press. Alternatively, the solids may be separated using a centrifugal filter pressor. The ated solids were then mixed with water to form a slurry and the slurry was heated to a temperature of about 150-250°C. The slurry was then subjected to supercritical water at about 374-600°C in a hydrolysis reactor for about 0.05-10 seconds under a pressure of about 230-300 bar. After exiting the hydrolysis reactor, the hydrolyzed slurry was quenched with water and then flashed to about ambient temperature and re to remove water. The lignin solids were then separated from the liquid stream using a centrifugal decanter and air dried.

The C5 oligosaccharides and the C6 oligosaccharides streams were first concentrated to about 200 g/L, adjusted to about pH 3-4 and filtered using 0.45 micron filter.

Sulfuric acid (0.3%) was added to the C5 oligosaccharides and the C6 oligosaccharides streams in a r reactor at a residence time of about 1 minute at about 200°C to hydrolyze the oligosaccharides to their respective monosaccharides. Alternatively, a batch autoclave at a lower temperature or a continuously stirred agitated tank reactor may be used. The glucose was cleaned with over liming and had acetic acid at a level of less than about 5 g/L. The xylose was cleaned by ting it with ion exchange resin and had acetic acid at a level of less than about 5 g/L.

Example 2: Analysis of monosaccharide compositions using inductively coupled plasma The dried compositions containing the C5 and C6 monosaccharides of Example 1 were analyzed using ively coupled plasma emission oscopy.

The results are shown in the table below: Monosaccharide Monosaccharide (glucose sample) (xylose sample) g/liter or ppm g/liter or ppm based on total C6 based on total C5 sacchaﬁdes sacchaﬁdes Monosaccharide Monosaccharide (glucose sample) (xylose ) r or ppm g/liter or ppm based on total C6 based on total C5 sacchaﬁdes sacchaﬁdes When ranges are used herein for physical properties, such as molecular weight, or chemical properties, such as chemical formulae, all combinations, and binations of ranges specific embodiments therein are ed to be included.

The disclosures of each patent, patent application, and publication cited or described in this document are hereby incorporated herein by reference, in their entirety.

Those skilled in the art will appreciate that numerous changes and modifications can be made to the red embodiments of the invention and that such changes and modifications can be made without departing from the spirit of the invention. It is, therefore, intended that the appended claims cover all such equivalent variations as fall within the true spirit and scope of the invention.

Claims

What is claimed is:

1. A composition, comprising: at least one water‐soluble C6 monosaccharide hydrolysate; at least one water‐soluble C6 oligosaccharide having a degree of polymerization of about 2 to about 15; and less than about 6750 ppm in total by weight, based on total weight of said ition comprising water‐soluble C6 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

2. A composition of claim 1, wherein said C6 monosaccharide hydrolysate is extracted from lignocellulosic biomass.

3. A composition of claim 2, wherein said C6 monosaccharide hydrolysate is processed from lignocellulosic biomass using supercritical, subcritical, or near critical fluid tion, or a combination thereof.

4. A composition of claim 1, r comprising: water.

5. A composition of claim 1, wherein said C6 monosaccharide hydrolysate is present at a concentration of at least about 0.5 g/L.

6. A composition of claim 1, wherein said C6 monosaccharide is e, galactose, mannose, fructose, or a mixture thereof.

7. A ition of claim 1, further comprising: 195105NZ_claims_20160317_PLH less than about 10 ppm by weight, based on total weight of said composition comprising water‐soluble C6 ride hydrolysate, of um; wherein said C6 monosaccharide hydrolysate is extracted from ellulosic biomass.

8. A composition of claim 1, r comprising: less than about 350 ppm by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of calcium; wherein said C6 monosaccharide hydrolysate is extracted from lignocellulosic biomass.

9. A composition of claim 1, further comprising: less than about 425 ppm by weight, based on total weight of said composition comprising water‐soluble C6 saccharide ysate, of iron; wherein said C6 monosaccharide hydrolysate is extracted from lignocellulosic

10. A composition of claim 1, further comprising: less than about 4500 ppm by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of sulfur; wherein said C6 monosaccharide hydrolysate is extracted from lignocellulosic biomass.

11. A composition of claim 1, wherein the weight ratio of said C6 monosaccharide hydrolysate to said elements is greater than about 25:1.

12. A composition of claim 1, wherein level of said elements are measured by inductively coupled plasma emission spectroscopy. 195105NZ_claims_20160317_PLH

13. A composition of claim 1, further comprising: less than about 1500 mg of nitrogen per kg of total weight of water‐soluble C6 saccharides.

14. A composition of claim 1, further sing: a weight ratio of the total mass of hydrogen and nitrogen to carbon of less than about 0.17.

15. A composition, comprising: at least one C6 monosaccharide hydrolysate; less than about 10 ppm by weight, based on total weight of said composition sing water‐soluble C6 saccharide hydrolysate, of aluminum; less than about 350 ppm by weight, based on total weight of said composition sing water‐soluble C6 saccharide hydrolysate, of calcium; less than about 425 ppm by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of iron; and less than about 4500 ppm by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of sulfur.

16. A ition of claim 15, further comprising: less than about 6750 ppm in total by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

17. A composition, sing: at least one C6 monosaccharide hydrolysate; and less than about 10 ppm by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of um.

18. A composition of claim 17, further comprising: 195105NZ_claims_20160317_PLH less than about 6750 ppm in total by , based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of elements; wherein said ts are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

19. A composition, comprising: at least one C6 monosaccharide hydrolysate; less than about 350 ppm by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of calcium; and less than about 6750 ppm in total by weight, based on total weight of said ition comprising water‐soluble C6 saccharide hydrolysate, of elements; n said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

20. A composition of claim 19, further comprising: less than about 6600 ppm in total by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

21. A composition, comprising: at least one C6 ccharide hydrolysate; less than about 425 ppm, based on total weight of said composition comprising water‐soluble C6 saccharide ysate, of iron; and less than about 6750 ppm in total by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

22. A composition of claim 21, further comprising: 195105NZ_claims_20160317_PLH less than about 6600 ppm in total by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

23. A composition, comprising: at least one C6 monosaccharide hydrolysate; and less than about 4500 ppm by weight, based on total weight of said composition comprising soluble C6 saccharide hydrolysate, of sulfur.

24. A composition of claim 23, further comprising: less than about 6750 ppm in total by weight, based on total weight of said composition comprising water‐soluble C6 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

25. A composition, comprising: at least one soluble C5 monosaccharide hydrolysate; and less than about 1950 ppm in total by weight, based on total weight of said composition comprising water‐soluble C5 saccharide ysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

26. A composition of claim 25, wherein said water‐soluble C5 ccharide hydrolysate is extracted from lignocellulosic biomass.

27. A ition of claim 26, wherein said water‐soluble C5 monosaccharide hydrolysate is processed from lignocellulosic biomass using ritical, subcritical, or near critical fluid extraction, or a combination thereof. 195105NZ_claims_20160317_PLH

28. A composition of claim 25, further comprising: water.

29. A composition of claim 25, wherein said C5 monosaccharide is present at a concentration of at least 0.5 g/L.

30. A composition of claim 25, wherein said water‐soluble C5 monosaccharide hydrolysate is xylose, arabinose, lyxose, , or a mixture thereof.

31. A composition of claim 25, further comprising: less than about 5 ppm by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of um.

32. A ition of claim 25, further comprising: less than about 300 ppm by , based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of calcium.

33. A composition of claim 25, further sing: less than about 10 ppm by , based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate of iron.

34. A composition of claim 25, further comprising: less than about 1000 ppm by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of sulfur.

35. A composition of claim 25, wherein the weight ratio of said C5 monosaccharide to said elements is greater than about 90:1. 195105NZ_claims_20160317_PLH

36. A composition of claim 25, wherein the level of said elements are measured by inductively coupled plasma emission oscopy.

37. A composition of claim 25, further comprising: less than about 350 mg of nitrogen per kg of total weight of water‐soluble C5 saccharides.

38. A ition of claim 25, further comprising: a weight ratio of the total mass of hydrogen and nitrogen to carbon of less than about 0.17.

39. A ition of claim 25, further comprising: at least one water‐soluble C5 oligosaccharide having a degree of polymerization of about 2 to about 15.

40. A composition, comprising: at least one water‐soluble C5 monosaccharide hydrolysate; less than about 5 ppm by weight, based on total weight of said ition comprising water‐soluble C5 saccharide hydrolysate, of aluminum; less than about 300 ppm by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of calcium; less than about 10 ppm, based on total weight of said ition comprising water‐soluble C5 saccharide ysate, of iron; and less than about 1000 ppm by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of sulfur.

41. A composition of claim 40, further comprising: less than about 1950 ppm in total by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of elements; 195105NZ_claims_20160317_PLH wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn.

42. A composition, comprising: at least one water‐soluble C5 monosaccharide hydrolysate; and less than about 5 ppm by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of aluminum.

43. A composition of claim 42, further comprising: less than about 1950 ppm in total by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn; and wherein said water‐soluble C5 monosaccharide hydrolysate is ted from lignocellulosic biomass.

44. A ition, comprising: at least one water‐soluble C5 monosaccharide hydrolysate; and less than about 300 ppm by weight, based on total weight of said composition comprising water‐soluble C5 ride hydrolysate, of calcium.

45. A composition of claim 44, r comprising: less than about 1950 ppm in total by weight, based on total weight of said ition comprising water‐soluble C5 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn; and wherein said water‐soluble C5 monosaccharide hydrolysate is extracted from ellulosic s.

46. A composition, comprising: at least one water‐soluble C5 monosaccharide hydrolysate; and 195105NZ_claims_20160317_PLH less than about 10 ppm by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of iron.

47. A ition of claim 46, further sing: less than about 1950 ppm in total by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn; and wherein said water‐soluble C5 monosaccharide hydrolysate is extracted from lignocellulosic biomass.

48. A composition, sing: at least one water‐soluble C5 monosaccharide ysate; and less than about 1000 ppm by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of sulfur.

49. A composition of claim 48, further comprising: less than about 1950 ppm in total by weight, based on total weight of said composition comprising water‐soluble C5 saccharide hydrolysate, of elements; wherein said elements are Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, Ni, P, Pb, S, Sb, Se, Si, Sn, Sr, Ti, Tl, V, and Zn; and wherein said water‐soluble C5 monosaccharide hydrolysate is extracted from ellulosic biomass. 195105NZ_claims_20160317_PLH