NZ626188B2 - Chimeric therapeutic anti - cd37 antibody hh1 - Google Patents

Abstract

The present invention relates an antibody molecule that binds to human CD37 and that is a chimeric antibody wherein the variable sequences are: a variable heavy chain comprising the amino acid sequence shown in SEQ ID NO: 1; and a variable light chain comprising the amino acid sequence shown in SEQ ID NO :3 (sequences as defined within the specification). ID NO :3 (sequences as defined within the specification).

Description

Chimeric therapeutic anti-CD37 antibody HH1 Technical field of the invention The present invention relates to immunotherapy and radioimmunotherapy of hematologic cancer with a chimeric or humanized antibody with an unexpectedly high cytotoxicity as well as various applications of the antibodies Background of the invention The present invention relates to chimeric and humanized anti-CD37 dies as well as the production and applications hereof.

The present invention furthermore relates to immunotherapies and radioimmunotherapies that are based on B-cell depletion.

In particular, the present invention relates to D37 antibody molecules for use in such therapies, e.g. in the ent of B cell ancies and autoimmune conditions.

Immunotherapy using monoclonal antibodies (mAbs) has been emerging as a safe and selective method for the treatment of cancer and other diseases.

In particular, the role of monoclonal dies in therapies that are based on B- cell depletion, e.g. in the treatment of B-cell ancies, has expanded since the introduction of rituximab, an antibody that is directed against the CD20 n on the B—celi surface.

The CD37 antigen is a cell surface antigen that has not been considered as a target for B cell malignancies to the same extent as the B-cell n CD20.

CD37, a member of the tetraspanin superfamily, is a heavily glycosylated cell surface molecule with four transmembrane domains and two extracellular loops.

CD37 expression is observed in normal B—cells, non—Hodgkin's lymphoma (NHL), including mantle cell lymphoma (MCL), Burkitts Lymphoma (BL), small cytic lymphoma (SLL) and follicular lymphoma (FL), marginal zone lymphoma (MZL), Diffuse large B-cell lymphoma (DLBCL), lymphoblastic lymphoma (LL), and chronic lymphoid leukemia (CLL).

This expression pattern makes CD37 an attractive target for antibody-mediated cancer therapy.

CD37 was first described in 1986 and terized by the murine monoclonal antibody MB-l (Link et al, 1986).

The physiological role of CD37 is unknown.

Binding of a CD37-specific mAb to cancer cells may trigger various isms of action: First, after the antibody binds to the extracellular domain of the CD37 antigen, it may activate the complement cascade and lyse the ed cell.

Second, an D37 dy may e antibody—dependent ediated cytotoxicity (ADCC) to the target cell, which occurs after the Fc portion of the bound antibody is recognized by appropriate receptors on cytotoxic cells of the immune system.

Third, the antibody may alter the ability of B-cells to respond to antigen or other Stimuli.

Finally, anti—CD37 antibody may initiate programmed cell death (apoptosis).

D37 mAb MB-l was evaluated in two radio-immunotherapy trials in B-NHL patients (B—cell non—Hodgkin’s lymphoma; Press et al., 1989; Kaminski et al., 1992).

Others have also disclosed anti—CD37 mABs that show potential (e.g. WO 2009/019312 by Heider et al. and by the present inventors) but there is still a long way to go before CD37 is proven the ideal alternative to CD20 for ng B-cell malignancies.

In conclusion, it has been shown that the CD37 antigen is frequently expressed on tumor cells in several human B—cell ancies and on mature normal B—lymphocytes and that anti—CD37—based y may be a promising approach for treating B cell malignancies.

Although the anti-CD37 antibodies or antibody—like les bed above (e.g. MB—l) have shown umor efficacy in B-cell malignancies and the potential to target CD37, there is a need for alternate anti-CD37 antibodies to improve therapies based on B-cell depletion.

Hence, an improved anti-CD37 antibody would be advantageous in the pursuit against new treatments against B—cell malignancies.

Summary of the invention An object of the present invention s to a chimeric or humanized antibody derived from the mouse monoclonal antibody HH1.

In particular, it is an object of the present invention to provide a chimeric or humanized antibody, or to at least provide a useful ative.

In a first aspect, the present invention provides an antibody molecule that binds to human CD37 and that is a chimeric antibody wherein the variable sequences are: (i) a variable heavy chain comprising the amino acid sequence shown in SEQ ID NO: 1; and (ii) a variable light chain comprising the amino acid sequence shown in SEQ ID NO :3. 3O One aspect of the present invention relates to an antibody molecule that binds to human CD37 and that is derived from a) a murine monoclonal antibody that is defined by i) a variable heavy chain comprising the amino acid sequence shown in SEQ ID NO: 1; and ii) a variable light chain comprising the amino acid sequence shown in SEQ ID NO :3, or from b) a non—human antibody izing the same epitope of human CD37 as the antibody defined in a) or recognizing an epitope that is close to or overlaps with said epitope; wherein said antibody molecule is a chimeric or a humanized antibody.

PCT/1132012/057230 Another aspect of the present invention relates to a DNA molecule encoding the antibodies of the present invention.

Yet another aspect of the present invention s to a host cell carrying one or more DNA molecules encoding the antibodies of the present invention.

Still another aspect of the present invention relates to a method for producing an antibody of the present invention, sing transfecting a mammalian host cell with one or more vectors encoding the antibodies of the present invention, culturing the host cell and recovering and purifying the antibody molecule.

Another aspect of the present ion relates to a pharmaceutical composition comprising, as the active ingredient, one or more antibodies of the present invention, and a ceutically acceptable carrier.

Another aspect of the present invention relates to a pharmaceutical composition for use in the ent of B-cell malignancies.

Yet another aspect of the present invention relates to method for treating a patient suffering from a B—cell malignancy ed from the group consisting of B- cell non—Hodgkins lymphoma, B—cell chronic lymphocytic ia, hairy cell leukemia, lymphoplasmacytic ma and multiple myeloma, comprising administering to said patient an effective amount of a pharmaceutical composition of the t invention.

Still r aspect of the present invention relates to a radioimmunoconjugate that binds human CD37 comprising a) an antibody of the present invention, b) a linker, and c) a radionuclide ed from the group consisting of 211At, 213Eii, 2128i, 212Pb, 225AC, 227Th, 90y, 186Re, 188Re, 199Au, 1941],, 166H0, 159Gd, 153Sm, 149Pm, 142Pr, lllAg, 109Pd, 77AS, 67CU, 475C, and 177LU- Another aspect of the present invention relates to a pharmaceutical composition comprising a radioimmunoconjugate as described above.

PCT/132012/057230 Another aspect of the present invention relates to the pharmaceutical as described above for use in the treatment of a B-cell malignancy selected from the group consisting of B-cell non-Hodgkins ma, B-cell chronic lymphocytic leukemia, hairy cell leukemia, lymphoplasmacytic lymphoma and multiple myeloma.

A further aspect of the present invention s to a method for treatment of a B- cell malignancy selected from the group consisiting of B—cell non—Hodgkins lymphoma, B—cell chronic lymphocytic leukemia, hairy cell leukemia, lymphoplasmacytic ma and multiple myeloma, comprising administration of an effective amount of a pharmaceutical composition of the t invention.

Yet another aspect of the present invention relates to a kit for the production of the radioimmunoconjugate of the present invention, comprising two or more vials, n one vial contains a conjugate comprising a chelator linked to an antibody of the present invention; and a second vial ns a radionuclide.

Also an aspect is the use of the chimeric antibody to block CD37 antigen in normal s before using radiolabeled murine or chimeric HH1 for therapy.

Brief description of the figures Figure 1 shows the flow cytometry clot plot for gating on the Daudi cells and the histogram for binding of anti—CD37 chHH1.1 (IgGl isotype) and no g of anti- NIP antibody (negative control). Figure 1 shows a significant antigen binding of chHHl.

Figure 2 shows the cellular binding of chHH1.1 t Daudi lymphoma cells. It shows a rapid and efficient binding to the target.

Figure 3 shows that chHHl has a similar ADCC as rituximab despite fewer antigens and a cant internalization of CD37 in the Daudi lymphoma target cells.

Figure 4 shows ADCC with IL-2 stimulated PBMC as effector cells and Rec-1 cells as target cells at three different ratios of effector to target cells. Mean and SD of three individuals.

Figure 5 shows CDC with 10 % serum from three individuals on Rec—1 cells labeled with murine HHl, mab, chHH1.1 or a combination of rituximab and ChHH1.1.

Figure 6 shows biodistribution (% I.D./g) of 125I—chHH1.3 in female nude mice 24 and 48 hours after injection.

Figure 7 shows tage specific lysis by ADCC in Daudi cells with NK cells as effector. The blood was taken from two individuals and one experiment was done with a ratio of 10 NK cells to 1 Daudi cells and the other experiment with a 15:1 ratio.

The present invention will now be described in more detail in the following.

Detailed description of the invention The present invention relates to zed or chimeric antibodies derived from the mouse monoclonal antibody HHl.

Humanized or chimeric antibodies are antibodies from man species whose protein sequences have been modified to increase their rity to antibody variants produced naturally in humans.

The process of "humanization" is usually d to monoclonal antibodies developed for stration to humans.

Humanization can be necessary when the process of developing a specific antibody involves generation in a non-human immune system (such as that in mice).

The protein sequences of antibodies produced in this way are partially distinct from homologous antibodies occurring naturally in humans, and are therefore potentially immunogenic when administered to human patients.

Not all monoclonal antibodies designed for human administration need be humanized since many therapies are short-term interventions.

Humanization is usually seen as distinct from the on of a mouse-human antibody chimera.

So, although the creation of an antibody chimera is normally undertaken to e a more human—like antibody (by substituting the mouse Fc region of the antibody with that from human) simple chimeras of this type are not usually referred to as humanized.

Rather, the protein sequence of a humanized antibody is essentially identical to that of a human variant, despite the non-human origin of some of its complementarity determining region (CDR) segments responsible for the ability of the antibody to bind to its target antigen.

However, in the present context the term chimeric antibody and humanized dy is used interchangeably as referring to an dy that has been genetically ered and is derived from the mouse monoclonal antibody HH1.

Chimeric or humanized antibodies derived from the monoclonal dy HH1 The immunoglobulin heavy chain (IgH) is the large polypeptide subunit of an dy oglobulin).

A typical antibody is composed of two globulin (Ig) heavy chains and two Ig light chains.

Several different types of heavy chain exist that define the class or isotype of an anfibody.

These heavy chain types vary n different animals.

The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin).

There are two types of light chain in humans (as in other mammals), kappa (K) chain, encoded by the immunoglobulin kappa locus on chromosome 2 and the lambda (A) chain, encoded by the immunoglobulin lambda locus on chromosome 22.

Antibodies are produced by B lymphocytes, each expressing only one class of light chain.

Once set, light chain class remains fixed for the life of the B lymphocyte.

In a healthy individual, the total kappa to lambda ratio is roughly 2:1 in serum ring intact whole antibodies) or 1:1.5 if measuring free light chains, with a highly divergent ratio indicative of neoplasm.

The exact normal ratio of kappa to lambda ranges from 0.26 to 1.65.

Both the kappa and the lambda chains can increase proportionately, maintaining a normal ratio.

Both variable and constant chains in a chimeric or humanized antibody derived from the mouse monoclonal antibody HH1 can differ from known sequences.

Examples of such ions are clear from the present disclosure and include 3O selection of constant chains, genetic variation of variable chains and variations of the Fc domain in order to modulate of effector functions.

The present inventors have cally ered chimeric, zed antibodies derived from the mouse onal antibody HH1.

These antibodies show a promising effect in the search for optimal treatment of B- cell related malignancies.

These effects are shown in the ments of the present disclosure.

Thus, an aspect of the present invention relates to an dy molecule that binds to human CD37 and that is derived from a) a murine monoclonal dy that is defined by i) a variable heavy chain comprising the amino acid sequence shown in SEQ ID NO: 1; and ii) a variable light chain comprising the amino acid ce shown in SEQ ID NO :3, or from b) a non-human antibody recognizing the same epitope of human CD37 as the antibody defined in a) or recognizing an epitope that is close to or overlaps with said epitope; n said antibody molecule is a chimeric or a humanized antibody.

In an embodiment of the present invention is the chimeric antibody defined by i) a variable heavy chain comprising the amino acid sequence shown in SEQ ID NO: 1; ii) a variable light chain comprising the amino acid sequence shown in SEQ ID NO:3, iii) constant heavy and light chains that are of human origin.

In another ment of the present invention is the chimeric antibody defined by i) the constant heavy chain is selected from the group consisting of IgG1, 1962, 1963 and IgG4 chain, and ii) the constant light chain is a kappa or a lambda chain.

In a further embodiment of the present invention is the constant heavy chain defined by i) comprising the amino acid sequence encoded by SEQ ID NO:5, SEQ ID N026 and/or SEQ ID N017 and wherein the constant light chain ii) comprises the amino acid sequence encoded by SEQ ID NO:9.

In a further embodiment of the present invention is the nt heavy chain defined by i) comprising the amino acid ce shown in SEQ ID NO:11 and/or SEQ ID NO:14 and wherein the nt light chain ii) comprises the amino acid sequence shown in SEQ ID NO:13.

PCT/IBZOlZ/057230 DNA 3 Fc sequence with mutation full length heavy chain can be seen as SEQ ID NO: 10.

Amino acid chHH1.3 Fc sequence with mutation full length light chain can be seen as SEQ ID NO:11.

DNA chHH1.3 Fc sequence with mutation full length heavy chain can be seen as SEQ ID NO: 12.

Amino acid chHH1.3 Fc sequence with mutation full length light chain can be seen as SEQ ID NO:13. chHH1.3 Fc sequence without mutation (constant region) can be seen as SEQ ID NO: 14.

Nucleic acid molecules encoding the dies of the present invention The humanization processes takes advantage of the fact that production of monoclonal antibodies can be accomplished using recombinant DNA to create constructs capable of expression in mammalian cell culture.

That is, gene segments capable of producing antibodies are isolated and cloned into cells that can be grown in a tank such that antibody proteins produced from the DNA of the cloned genes can be harvested en masse.

The step involving recombinant DNA provides an ention point that can be readily exploited to alter the protein sequence of the expressed antibody.

The alterations to antibody ure that are achieved in the humanization process are therefore all uated through ques at the DNA level.

Thus, an aspect of the present invention relates to a DNA molecule encoding the humanized or chimeric antibodies of the present invention.

In one embodiment of the t invention encodes the DNA molecule a region encoding the variable heavy chain of a humanized or chimeric antibody of the present invention.

In another embodiment of the present invention is this variable heavy chain encoding region fused to a region encoding a constant heavy chain of human origin.

Such human constant heavy chain can be selected from the group consisting of 1961, 1962, IgG3 and IgG4.

In one embodiment of the present invention is the IgGl encoded by the sequence shown in SEQ ID NO:5, SEQ ID NO:6 and/or SEQ ID NO:7 In one embodiment of the present invention is the 1963 heavy chain encoded by the sequence shown in SEQ ID NO:10.

In one embodiment of the present invention is the 1963 light chain encoded by the sequence shown in SEQ ID NO:12.

In one embodiment of the present invention is the 1963 with H435 tution on encoded by the sequence shown in SEQ ID NO:12.

In a further embodiment of the present invention comprises the human nt heavy chain one or more substitutions in the Fc region.

Another embodiment of the present invention relates to a DNA molecule comprising a region encoding the variable light chain of the ic or zed antibody of the present invention.

Such variable light chain encoding region may be fused to a region encoding a constant light chain of human .

The constant light chain may be a kappa or a lambda chain.

In one embodiment of the present invention is the kappa light chain encoded by a sequence shown in SEQ ID N029.

The DNA molecules can be constructed and optimized for sion in a target cell.

An expression vector, otherwise known as an expression construct, is lly a plasmid that is used to introduce a specific gene (in the present context the DNA molecule of the t invention) into a target cell.

Once the expression vector is inside the cell, the protein that is encoded by the gene is produced by the cellular—transcription and translation machinery ribosomal complexes.

The plasmid is ntly engineered to contain regulatory ces that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector.

The goal of a well—designed expression vector is the production of large amounts of stable messenger RNA, and therefore ns.

Expression vectors are basic tools for biotechnology and the production of proteins such as insulin that are important for medical treatments of specific es like diabetes.

After expression of the gene product, the purification of the protein is required; but since the vector is introduced to a host cell, the protein of interest should be purified from the proteins of the host cell. 3O Therefore, to make the purification process easy, the cloned gene should have a tag. This tag could be histidine (His) tag or any other marker peptide.

Thus, an aspect of the t invention therefore s to an expression vector comprising a DNA molecule as described above.

ZOIZ/057230 Cells comprising and expressing the antibodies of the present invention The above described DNA molecules or expression vectors can be introduced into host cells.

Such cells can through oma technology become alized cells that produce the chimeric or humanized antibodies.

Hybridoma technology is a logy of forming hybrid cell lines (called hybridomas) by fusing a specific dy—producing B cell with a myeloma (B cell ) cell that is selected for its y to grow in tissue culture and for an absence of antibody chain synthesis.

The antibodies produced by the hybridoma are all of a single specificity and are therefore monoclonal antibodies (in contrast to polyclonal antibodies).

Thus, one aspect of the t invention relates to a host cell carrying one or more vectors or DNA molecules of the present invention.

One embodiment of the present invention s to a host cell carrying an expression vector comprising a DNA molecule encoding the variable heavy chain of HHl, and a second expression vector comprising a DNA molecule encoding the variable light chain of the present invention.

In one embodiment of the present invention is the host cell a mammalian cell.

In another embodiment of the present invention is the cell a hybridoma cell.

Methods for producing the antibodies of the present invention The chimeric or humanized antibodies of the present invention can be produced by several methods.

One method for producing such antibodies comprises transfecting a mammalian host cell with one or more vectors of the present invention, culturing the host cell and recovering and purifying the antibody le.

Another method for producing such antibodies comprising construction of hybridoma cells that produce the chimeric or humanized antibodies of the present invention.

Sequence ty As commonly defined "identity" is here defined as sequence identity n genes or proteins at the tide or amino acid level, respectively.

Thus, in the present context "sequence ty" is a measure of identity between proteins at the amino acid level and a measure of identity between nucleic acids at nucleotide level.

The n sequence identity may be determined by comparing the amino acid sequence in a given on in each sequence when the sequences are aligned.

Similarly, the nucleic acid sequence identity may be determined by comparing the nucleotide ce in a given position in each sequence when the sequences are aHgned.

To determine the percent identity of two nucleic acid sequences or of two amino acids, the sequences are aligned for optimal comparison purposes (e.g., gaps may be introduced in the ce of a first amino acid or nucleic acid sequence for optimal alignment with a second amino or nucieic acid ce). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared.

When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent identity between the two PCT/[82012/057230 sequences is a function of the number of identical positions shared by the sequences (i.e., % identity = # of identical positions/total # of positions (e.g., overlapping positions) x 100). In one ment the two sequences are the same length.

One may manually align the ces and count the number of identical c acids or amino acids. Alternatively, ent of two sequences for the ination of percent identity may be accomplished using a mathematical algorithm. Such an algorithm is incorporated into the NBLAST and XBLAST programs. BLAST nucleotide searches may be performed with the NBLAST program, score = 100, wordlength = 12, to obtain nucleotide sequences homologous to a nucleic acid molecules of the invention. BLAST protein searches may be performed with the XBLAST program, score = 50, wordlength = 3 to obtain amino acid sequences homologous to a protein molecule of the invention.

To obtain gapped alignments for comparison purposes, Gapped BLAST may be ed. Alternatively, PSI—Blast may be used to perform an iterated search which detects t relationships between molecules. When utilising the NBLAST, XBLAST, and Gapped BLAST programs, the default parameters of the respective programs may be used. See http://www.ncbi.nlm.nih.gov. Alternatively, sequence identity may be calculated after the sequences have been aligned e.g. by the BLAST program in the EMBL database (www.ncbi.nlm.gov/cgi—bin/BLAST).

Generally, the default settings with respect to e.g. “scoring matrix" and “gap penalty” may be used for alignment. In the context of the t invention, the BLASTN and PSI BLAST default gs may be advantageous.

The percent identity between two sequences may be determined using techniques similar to those described above, with or without allowing gaps. In ating 3O percent identity, only exact matches are counted.

An embodiment the invention relates to an isolated nucleic acid comprising a nucleic acid sequence g 80 % sequence identity with the HH1 antibody VH ce (SEQ ID NO: 2) and/or VL sequence (SEQ ID NO: 4). 2012/057230 An ment the invention relates to an isolated nucleic acid comprising a nucleic acid ce with the HH1 antibody VH sequence (SEQ ID NO: 2) and/or VL sequence (SEQ ID NO: 4).

In another embodiment of the invention the isolated nucleic acid comprises a nucleic acid sequence sharing at least 90 % sequence ty with the HH1 antibody VH sequence (SEQ ID NO: 2) and/or VL sequence (SEQ ID NO: 4), such as 90 % identity, 91 % identity, 92 % identity, 93 0/0 identity, 94 % identity, 95 % identity, 96 % identity, 97 % identity, 98 % identity, or 99 % identity. r embodiment of the invention relates to an antibody comprising a polypeptide sequence g 80 % sequence identity with the HH1 antibody VH sequence (SEQ ID NO: 1) and/or VL sequence (SEQ ID NO: 3).

Another embodiment of the invention relates to an dy comprising a polypeptide sequence with the HH1 antibody VH sequence (SEQ ID NO: 1) and/or VL sequence (SEQ ID NO: 3).

In another embodiment of the present invention, the antibody comprises a polypeptide sequence sharing at least 90 % sequence identity with the HH1 antibody VH sequence (SEQ ID NO: 1) and/or VL ce (SEQ ID NO: 3), such as 90 % identity, 91 % identity, 92 % identity, 93 % identity, 94 % identity, 95 % identity, 96 % identity, 97 % identity, 98 % ty, or 99 % identity.

In a preferred embodiment of the present invention are these antibodies a chimeric or humanized antibody d from the monoclonal antibody HH1.

Genetic variation Genetic variation is caused by variation in the order of bases in the nucleotides in genes. This variation cause mutations in the genes and subsequently in the ns that such genes encode.

These mutations can be either sense or missense mutations or substitutions. 2012/057230 An ment of the present invention relates to the isolated nucleic acid sequence of the HH1 monoclonal antibody VH chain (SEQ ID NO: 2) and/or VL chain (SEQ ID NO: 4) that ses at least 50, such as 20, such as 10, such as , such as 4, such as 3, such as 2, such as 1 sense mutations.

Another embodiment of the t invention s to the isolated nucleic acid sequence of the HH1 monoclonal antibody VH chain (SEQ ID NO: 2) and/or VL chain (SEQ ID NO: 4) that comprises 0-50, such as 1-50, such as 0-20, such as 1- , such as 0—10, such as 1—10, such as 0-5, such as 1-5, such as 3, such as 1 sense mutations.

A missense mutation (a type of non-synonymous mutation) is a point mutation in which a single nucleotide is changed, resulting in a codon that codes for a different amino acid ions that change an amino acid to a stop codon are considered nonsense mutations, rather than missense mutations). A missense on can render the resulting protein non—functional.

However, not all missense ons lead to appreciable protein changes. An amino acid may be replaced by an amino acid of very similar chemical properties, in which case, the protein may still function normally; this is termed a neutral, "quiet", or conservative mutation.

Alternatively, the amino acid substitution could occur in a region of the protein which does not significantly affect the protein secondary structure or function.

When an amino acid may be encoded by more than one codon (so—called "degenerate coding") a mutation in a codon may not produce any change in translation; this would be a synonymous mutation (a form of silent mutation) and not a se mutation.

An embodiment of the present invention relates to an antibody comprising a polypeptide sequence of the HH1 monoclonal antibody VH chain (SEQ ID NO: 2) and/or VL chain (SEQ ID NO: 4) that comprises at least 50, such as 20, such as , such as 5, such as 4, such as 3, such as 2, such as 1 missense mutations.

An embodiment of the present invention relates to an antibody comprising a polypeptide sequence of the HHl monoclonal antibody VH chain (SEQ ID NO: 2) and/or VL chain (SEQ ID NO: 4) that comprises 0-50, such as 1-50, such as 0-20, such as 1-20, such as 0-10, such as 1-10, such as 0-5, such as 1-5, such as 3, such as 1 missense mutations.

A conservative substitution is a substitution of one amino acid with another with generally similar properties such that the overall functioning is likely not to be seriously affected.

In another embodiment of the present invention are the missense mutations conservative mutations or substitutions.

A further embodiment of the present invention relates to an isolated nucleic acid sequence or a polypeptide sequence with 80% sequence identity to the variable heavy chain (SEQ ID NO: 1) and/or variable light chain (SEQ ID NO: 3) sequences af HHl, wherein the sequence ion is conservative substitutions.

In another embodiment of the t invention is the sequence identity 80% ty, such as 90% identity, 91 % ty, 92 % ty, 93 % identity, 94 % identity, 95 % identity, 96 % identity, 97 % identity, 98 % identity, or 99 % identity and the sequence variation is conservative substitutions.

In order to improve the radiolabeling step it may be cial to introduce extra lysine into e.g., the Fc portion of the chimeric or zed dy of the present invention.

This could reduce the probability of attaching lysine binding chelators into the antigen combining sites at the antibody, thereby ng the risk of compromizing immunoreactivity during radiolabeling.

Methods for introducing lysine into eg. the Fc n of HHl is known in the art e.g. from Hemminki et al., 1995.

An embodiment of the present invention relates to the radioimmunoconjugate of the present invention which has been modified by 10 Lys in the Fc portion of HH 1, such as 8 Lys, such as 6 Lys, such as 5 Lys, such as 4 Lys, such as 3 Lys, such as 2 Lys, such as 1 Lys.

Other variations of the Fc portion of the antibodies of the present invention can be chosen in order to optimize or modulate one or more effector functions.

These modulations of effector functions are made e.g. to increase in dy— dependent cell-mediated cytotoxicity (ADCC).

Such ions of the Fc portion are known in the art.

Thus, one aspect of the present invention relates to a antibody of the present invention that has one or more mutations in the Fc domain that modulate one or more effector functions.

Immonoconjugates Immunoconjugates are antibodies conjugated (joined) to a second molecule, usually a toxin, radioisotope or label.

Such immunoconjugates of chimeric and humanized HH1 are all s of the t invention.

One type is the chimeric and humanized HH1 of the present ion ted to or ated with a chelating linker.

Chelating linkers are discussed in the below section regarding 3O radioimmunoconjugates and the chelating linkers described therein are therefore all considered useful for conjugates comprising chimeric and humanized HH1 of the present invention connected to or associated with a chelating linker Radioimmunoconjugates An aspect of the present invention relates to a radioimmunoconjugate that binds human CD37 comprising a chimeric or humanized antibody d from the monoclonal antibody HH1 according to the present invention, a linker, and a radionuclide selected from the group consisting of 211At, 213Bi, 212Bi, 212%, 225Ac, 227Th, 90Y’ 186Re, 188Re’ 199AU, 194Ir, 166HO, lngd, 153Sm, 149Pm, 142Pl', lllAg, 109Pd, 77As, 67Cu, 47Sc, and 177Lu.

In an embodiment of the present ion the linker is a chelating .

In another embodiment of the present invention is the radionuclide selected from the group consisting of 177Lu, 225Ac, 227Th and 90Y.

In another embodiment of the present invention the radionuclide is 177Lu.

In another embodiment of the present invention the radionuclide is 212%.

In yet r embodiment the radionuclide is another beta-emitter or an alpha- emitter.

The radionuclide may be attached to the dy by first reacting a bifunctional chelator, e.g., p—SCN-bn—DOTA (Macrocyclics, TX, USA), with the antibody, followed by cation to remove unconjugated chelator, and then reaction of the chelator antibody conjugate with the radionuclide, followed by purification to remove any unconjugated uclide.

Alternatively, the chelator and the radionuclide can be combined firstly and uently conjugated to the antibody.

Chelating linkers like, e.g., p-SCN—bn-DOTA, can be used for conjugating other metal radionuclides to HH1 derived antibodies in similar fashion to that described for 177Lu.

Any type of linker with sufficient complexing ability and a functional group ng direct or indirect conjugation to a protein or a peptide could be used.

Examples of such linkers are described in the literature (e.g. Brechbiel, 2008; Liu, 2008). Some useful examples are bifunctional cyclic chelators like p—SCN—bn- DOTA, DOTA-NHS-ester, p-SCN—Bn-TCMC; bifunctional linear chelators like p- SCN-Bn-DTPA and CHX-A"-DTPA.

The radionuclides in the present invention will preferably be ated to a targeting le by using bifunctional ors.

These could be cyclic, linear or branched chelators. Particular reference may be made to the polyaminopolyacid chelators which se a linear, cyclic or branched polyazaalkane backbone with acidic (e.g. carboxyalkyl) groups attached at backbone nitrogens.

Examples of suitable chelators include DOTA derivatives such as p- isothiocyanatobenzyl—1,4,7,lO-tetraazacyclododecane-1,4,7,10—tetraacetic acid (p-SCN-Bz-DOTA) or S—2-(4-Isothiocyanatobenzyl)-1,4,7,10—tetra(2— carbamoylmethyl)cyclododecane and DTPA derivatives such as p— isothiocyanatobenzyl—diethylenetriaminepentaacetic acid (p-SCN-Bz-DTPA), the first being cyclic ors, the latter linear chelators.

Metallation of the complexing moiety may be performed before or after conjugation of the complexing moiety to the targeting moiety.

The radiolabeling procedure will in general be more convenient in terms of time used etc if the chelator is conjugated to the antibody before the radiolabeling takes place.

The principles of preparing radiolabeled conjugates using chelators attached to antibodies are described broader in e.g. Liu, 2008.

Thus, HH1 derived chimieric or humanized antibodies can be used to prepare mmunoconjugates with differences in radiation ties and effective half- lives.

For example anti—CD37 radiolmmunoconjugate consisting of a chimeric or humanized antibody derived from the monoclonal dy HHl according to the present ion, a chelating linker and a beta or alpha emitting radionuclide including, but not limited to 177Lu, 211At, 2133i, 2128i, 212Pb, ZZSAC, 227Th, 9°Y, 186Re, 188Re, 199Au, 194D”, 166H0, 159Gd, 153Sm, 149Pm, 142Pr, lllAg, 109Pd, 77AS, 67CU, 475C can be prepared and used for preparing pharmaceutical preparations and used in eutic ations.

Immunotoxins An immunotoxin is a human—made protein that consists of a targeting portion linked to a toxin. When the protein binds to that cell, it is taken in through endocytosis, and the toxin kills the cell.

They are y used for the treatment of some kinds of cancer and a few viral infections.

These proteins are usually made of a modified antibody or antibody fragment, attached to a fragment of a toxin.

The targeting portion is composed of the Fv portion of an dy that targets a specific cell type.

The toxin is usually a cytotoxic protein derived from a ial or plant protein, from which the natural binding domain has been removed so that the Fv directs the toxin to the antigen on the target cell.

In another embodiment of the invention the toxin is a chemotherapeutic molecule, including, but not limited to alkylating agents (cisplatin, carboplatin, oxaliplatin, mechlorethamine, hosphamide, chlorambucil, ifosfamide), anti-metabolites (azathioprine, mercaptopurine, pyrimidines), alkaloids (vincristine, vlnblastine, cinorelbine, vindesine, paclitaxel, docetaxel, etoposide, teniposide), topoisomerase inhibitors tecan, topotecan, ine, etoposide, teniposide) and cytotoxic antibiotics (actinomycin, doxorubicin, daunorubicin, calrubicin, idarubicin, epirubicin, bleomycin, plicamycin, mitomycin).

In one embodiment of the invention doxorubicin is conjugated to the antibody via the cross—linker SMCC-hydrazide (4-[N—maieimidomethyl]cyclohexane—1 carboxylhydrazide).

The immunotoxin works by the antibody (or other targeting moiety) binding to an antigen on the target cell followed by toxin that enters and kills the cell.

Thus, an aspect of the present invention relates to an immunotoxin that comprises the antibody of the present invention or a fragment hereof.

Pharmaceutical compositions Antibodies are usually d in the treatment of diseases formultated in pharmaceutical compositions.

Such compositions are optimized for parameters such as logical tolerance and shelf—life.

Thus, relates and aspect of the t ion to a pharmaceutical composition comprising, as the active ingredient, one or more anti-CD37 antibody les of of the present invention (i.e. the chimeric or humanized antibody derived from HH1 or a fragment hereof), and a pharmaceutically acceptable carrier.

An ment of the present invention relates to a pharmaceutical composition as bed above, further comprising one or more additional therapeutic agents.

In one ment of the present invention are said one or more additional therapeutic agents are selected from agents that target a B-ceii antigen other than CD37.

Such n may be the B-cell antigen CD20.

In another embodiment of the t invention are said one or more onal therapeutic agents ed from agents that induce apoptosis.

A radioimmunotherapeutic product based on chimeric or humanized HH1 would typically be provided as a ceutical composition consisting of a radionuclide, according to the description above, linked via a chelator to the chimeric or humanized antibody HH1 dissolved in a buffer solution, which to a ntial degree maintain the chemical integrity of the radioimmunoconjugate and is being physiologically acceptable for infusion into patients.

Thus, an aspect of the present invention relates to a pharmaceutical composition comprising a radioimmunoconjugate of the present ion, and a pharmaceutically acceptable carrier and/or excipient.

Acceptable pharmaceutical carriers include but are not limited to non-toxic buffers, s, isotonic solutions, etc. More specifically, the pharmaceutical carrier can be but are not limited to normal saline (0.9 0/o), half-normal saline, Ringer’s lactate, 5 % Dextrose, 3.3 % Dextrose/0.3 % Saline. The logically acceptable carrier can contain a radiolytic stabilizer, e.g., ascorbic acid, which protect the integrity of the radiopharmaceutical during storage and shipment.

One embodiment of the present invention comprises the pharmaceutical composition of the present invention and one or more additional antibodies or radioimmunoconjugates. Antibodies include but are not limited to Rituximab, Epratuzumab, L19, F8, F16, Galiximab, Toralizumab, Alemtuzumab, umab, Veltuzumab, umab, momab, Reditux, Ibritumomab, , 37.1 and HH1. 3O Radioimmunoconjugates include but are not limited to Zevalin, Bexxar and Betalutin.

In another embodiment of the present invention one or more additional antibodies or radioimmunoconjugates target CD20. Antibodies e but are not limited to Rituximab, Veltuzumab, Ofatumumab, Afutuzumab, Tositumomab, Reditux and Ibritumomab. Radioimmunoconjugates include but are not limited to Zevalin and Bexxar.

A further embodiment of the present ion s to a ceutical composition of the present invention for treating B—cell malignant cells expressing the CD37 antigen.

In an embodiment of the present invention the pharmaceutical composition is for treatment of a B-cell malignancy ed from the group consisiting of B-cell non- Hodgkins lymphoma, B-cell c lymphocytic leukemia, hairy cell ia, small lymphoblastic lymphoma and multiple myeloma.

Treatment Therapeutic use of a pharmaceutical solution according to the present invention may be for treatment against malignant cells expressing the CD37 antigen, including but not limited to a B—cell ancy selected from the group consisiting of B-cell non-Hodgkins ma, B-cell chronic lymphocytic leukemia, hairy cell leukemia, lymphoplasmacytic lymphoma and le myeloma.

Other uses could be treatment of autoimmune es and treatment of transplantation related effects.

The therapy could be based on, but are not limited to, article-radiation or alpha-particle—radiation or a combination of these.

The therapy could be administered either as a monotherapy or in combination with other therapies, preferentially standard treatments. Such other therapies may be pretreatment, surgery, chemotherapy (including doxorubicin, vinblastin and gemcitabine), immunotherapy, photodynamic therapy, proteasome tor (including bortezomib), histone deacetylase inhibitors (including vorinostat and suberoylanilide hydroxamic acid), vitamin D3 and vitamin D3 analogs, cell cycle checkpoint inhibitors (including UCN-Ol and 2-(4—(4—Chlorophenoxy)phenyI)-1H- benzimidazole-S-carboxamide), hypoxic cell radiosensitizers (including PCT/IB201Z/057230 metronidazole and misonidazole), apoptosis inducers ding withaferin A) radiosensitizers, radioimmunotherapy or a ation of two or more of these.

By administered is meant intravenous infusion or intravenous injection. More specifically, the radioimmunoconjugate of the present invention can be administered directly in a vein by a peripheral a connected to a drip chamber that prevents air embolism and allows an estimate of flow rate into the patient.

In one embodiment the mmunoconjugate can be administered in a repeated fashion.

In another embodiment of the present invention the radioimmunoconjugate could be administered in a repeated n but with different uclides, e.g., beta- radioimmunotherapy could be followed by alpha-radioimmunotherapy or vice versa.

An aspect of the present invention relates to the use of the radioimmunoconjugate of the present invention for the treatment of B—cell malignancies.

An embodiment of the present invention relates to the use of the radioimmunoconjugate of the present invention administered in combination with or in addition to other therapy.

In an embodiment of the present invention the other therapies is ed from pretreatment, chemotherapy, monoclonal dy therapy, surgery, radiotherapy, and/or photodynamic therapy.

In another embodiment of the present invention the other therapies are bone 3O marrow transplantation or stem cell transplantation and/or therapy.

Another embodiment of the present invention comprises therapeutic pre- treatment using DZO and/or anti-CD37 monoclonal antibody prior to the treatment with the radioimmunoconjugate of the t invention.

PCT/IBZOIZ/057230 In an embodiment of the present ion is the pretreatment done by administering the chimeric antibody of the present invention ed by treatment by radioimmunoconjugates of the chimeric antibody of HH1 or radioimmunoconjugates of the antibody HH1.

An aspect of the present invention relates to a method for treatment of a B-cell malignancy selected from the group consisiting of B-cell non-Hodgkins ma, B—cell chronic Iymphocytic ia, hairy cell ia, plasmacytic lymphoma and multiple myeloma, sing administration of an effective amount of the pharmaceutical composition of the present invention.

In one embodiment of the present invention are the uses and s of treatment of the present invention performed in vitro or ex vivo.

In an embodiment of the present invention the antibody dosing is 1—1000 mg per patient, more preferably 5-50 mg per t, and 177Lu amounting to 1 — 200 , more preferably 10-100 MBq/kg of bodyweight.

The ceutical compositions of the present invention comprising the chimeric or humanized antibody of the present invention can be used in depleting B cells that express CD37 on their surface.

Such pharmaceutical compositions can be used in the treatment of B—cell malignancies.

These B-cell malignancies may be selected from the group consisting of B—cell non—Hodgkins lymphoma, B~cell chronic Iymphocytic leukemia, hairy cell leukemia, lymphoplasmacytic lymphoma and multiple myeloma.

An embodiment of the present invention relates to a pharmaceutical composition comprising the chimeric or humanized antibody of the present invention for use in the treatment of autoimmune or inflammatory diseases that involve B-cells in their pathology. r embodiment of the present invention relates to a method of depleting CD37 expressing B-cells from a population of cells, comprising administering to said population of cells an antibody molecule of the present invention a pharmaceutical composition containing such antibody le.

Yet another embodiment of the present invention s to a method for treating a patient suffering from a B-cell malignancy ed from the group consisiting of B-cell non-Hodgkins lymphoma, B—cell chronic lymphocytic leukemia and multiple myeloma, comprising administering to said patient an effective amount of a ceutical composition the chimeric or humanized antibody of the present invention.

In a special embodiment a chimeric or humanized HH1 is administered to a patient, prior to therapy with a radiolabeled or an immuntoxin version of , ic or zed HH1, to block normal tissue cells and improve tumor uptake.

The dosing should be sufficient to block normal tissue uptake but not excessive as this would reduce the tumor uptake. E.g. the chimeric or humanized HH1 should be given in a dosage between 0,5 mg and 1 g per patient.

It could be given e.g. one week before and /or 1—5 hours before the administration of the radioimmunoconjugate.

Kits An aspect of the present invention relates to a kit for the production of the radioimmunoconjugate of the present invention comprising two or more vials, wherein one vial contains a ate comprising a chelator linked to a murine monoclonal antibody HH1; and a second vial contains a radionuclide.

A kit may require some procedures to be performed, e.g., radiolabeling and/or purification to take place before infusion.

PCT/1132012/057230 An embodiment of the present invention relates to a kit of the present invention, wherein the content of one or l of the vials are either lyophilized or in a solution.

By mixing the contents of the two vials to generate the radioimmunoconjugate the final product will appear. Thus, in another embodiment of the present invention the radioimmunoconjugate is generated by mixing the content of the two vials.

This product may need purification prior to use.

It should be noted that embodiments and features described in the context of one of the aspects of the present invention also apply to the other aspects of the invenfion.

All patent and non-patent references cited in the t application, are hereby incorporated by reference in their entirety.

The invention will now be described in further details in the following non-limiting examples.

Examples Example 1 Generation of ic antibody A chimeric version of the HHl antibody was made using expression vectors for the V-region gene.

The vectors accept VH and VL chain genes obtained by RT—PCR.

The V-genes were then ced and new specific primers were designed to amplify the V-genes before they were cloned into the pLNOHZ vector ning the human IgG antibody constant region genes (Cy3, CV1 and CH1v3).

The pLNOHZ vector ing the constant regions l (SEQ ID NO. 5), Z (SEQ ID NO. 6), IgGlCH3 (SEQ ID NO. 7) is shown in SEQ ID NO. 8.

The heavy chain and the light chain of the vectors pLNOH2 (SEQ ID NO. 5) and pLNOK were combined to make the combi vector pLNOH2y1/K dCD37. The complete light chain gene (SEQ ID NO:9), the CMV promoter and polyA signal of pLNOK qCD37 were subcloned over to pLNOH2 hIgGl ClCD37.

In the combi vector, the heavy and light chains are expressed from their own CMV promoter.

Identification of constant domain The constant domains in the pLNOH2 sequence were identified from the ce of a human IgGl in the IMGT database (accession number: 217370).

The gene and protein sequence of the variable regions of the chHHl anti—CD37 antibody is as follows: VH dCD37 (aminoacid sequence: SEQ ID NO: 1 and c acid sequence: SEQ ID NO: 2) c acid sequence (SEQ ID NO: 2): gagatccagctgcagcagtctggacctgagctggtgaagcctggggcttcagtgaaggtatcctgcaaggcttctg gttactcattcactgactacaacatgtactgggtgaagcagagccatggaaagagccttgagtggattggatatatt gatccttacaatggtgatactacctacaaccagaagttcaagggcaaggccacattgactgttgacaagtcctccag cttcatccatctcaacagcctgacatctgaggactctgcagtctattactgtgcaagatccccttatggtcact atgctatggactactggggtcaaggaacctcagtcaccgtctcctca Amino acid sequence (SEQ ID NO: 1): EIQLQQSGPELVKPGASVKVSCKASGYSFTDYNMYWVKQSHGKSLEWIGYIDPYNGD'I‘I'YN QKFKGKATLTVDKSSSTAFIHLNSLTSEDSAVYYCARSPYGHYAMDYWGQGTSVTVSS 3O VL dCD37 (aminoacid sequence: SEQ ID NO: 3 and nucleic acid sequence: SEQ ID NO: 4) Nucleic acid sequence (SEQ ID NO: 4): gacattgtgatgacccagtctcacaaactcttgtccacatcagtaggagacagggtcagcatcacctgcaaggcca atgtgagtactgctgtagactggtatcaacagaaaccaggacaatctcctaaactactgattaactgggca tccacccggcacactggagtccctgatcgcttcacaggcagtggatctgggacagattatactctcaccatcagcagt WO 88363 atg caggctgaagacctggcactttattactgtcgacaacattatagcactccattcacgttcggctcggggacaaag ttggaaataaaa Amino acid sequence (SEQ ID NO: 3): DIVMTQSHKLLSTSVGDRVSITCKASQDVSTAVDWYQQKPGQSPKLLINWASTRHTGVPD RFI‘GSGSGTDYTLTISSMQAEDLALYYCRQHYSTPFTFGSGTKLEIK Example 2 Flow cytometry to evaluate antigen binding Analysis of l oaCD37 b(chHH1) binding to CD37 expressing Daudi cells Cells stained and fixated.

The constructed chHHl was analyzed for target binding by flow cytometry. Daudi cells express CD37, and the cells were stained with either chHHl or hIgGl aNIP.

In the left panel the intact Daudi cells are ed, and in the right panel the fluorescence histogram of these cells d are shown. chHHl bound to the CD37—expressing Daudi cells (solid line) while the chimeric oNIP 1961 did not (dotted line).

Example 3 Radiolabeling of ic HHl Iodination: Antibodies were labeled with 125I through indirect iodination using IODOGEN pre—coated iodination tubes (Pierce, Rockford, IL) according to the manufacturer’s description.

Labeling with 111m and 177Lu: Antibodies were first reacted with a or (p- SCN-Bn—DTPA or p—SCN—Bn-DOTA).

The DTPA or DOTA chelator was dissolved in 0.05 M HCI, and then added to the dy, which was pH-adjusted to approximately 8,5 by washing with carbonate buffer, in a 5:1 ratio. pH was then checked again and if necessary adjusted. The solution was shaken in 60 min at room temperature, and then the reaction was terminated by adding 50 pl 200 mM glycine solution (per mg antibody).

To remove free or the conjugated antibody was washed 4-5 times with PBS (PAA), and then adjusted to pH 5 by washing with ammonium acetate. 111In or 177Lu (Perkin Elmer, Boston, Ma, USA) was then added to 0.5 mg DOTA-Ab, and shaken for one hour at 420C.

Finally, the t was purified by elution on a gel filtration column, e.g., Sephadex G—25 PD10 (GE health) or similar. The overall labeling yield varied from 17 % to 63 %.

The quality of the mmunoconjugates was measured using ma cells and a modified Lindmo method (Example 4).

Example 4 Immunoreactivity Background: Immunoreactivity analysis was used to measure the antibody’s ability to bind to antigen-positive target cells.

Methods: Two parallelsof 5, 10, 40, 100 09 300 million cells pr.ml in approximately 0.3 ml were used.

Half of the tubes were added led antibody to a concentration of 0.5 mg/mi and incubated for 15 min. to block the antigen.

Thereafter, 5-20 ng/ml radioimmunoconjugate was added to all tubes and the suspension incubated at 40C using a shaker for approx. 2 hours.

Thereafter the tubes were centrifuged and the supernatant removed and stored for counting. The pellets were re—suspended in 1 ml PBS and centrifuged. This washing procedure was repeated twice and the atants pooled. Tubes containing the pellets or the pooled atants were counted on a gamma counter.

The specific bound activity was calculated using the following: Pellet counts nonblocked (PCN), combined supernatant counts (CSC) and the total applied (TA) for each sample. Specific binding (SB) was calculated as follows: A) — (PCN/TA)blocked = SB.

The SB values were calculated for each cell concentrations and plotted in a double reciprocal plot and the immunoreactive fraction at te antigen access was determined according Lindmo et al (J Immunol Methods. 1984 Aug 3;72(1):77- 89).

Results: Measurements of two batches of 177Lu-labeled chimeric HH1 gave immunoreactivities of 48.1% and 84.5% respectively. One batch of 125I-labeled chimeric HH1 gave an immunoreactivity of 67.6%. In conclusion these data are in good agreement with those lly found for the murine HH1 and that show that the chimeric HH1 has a relevant antigen-targeting ability.

Example 5 Binding to tumor cells in vitro Introduction: A binding assay was performed in order to determine the equilibrium binding constand (Kd) of chHHl, the e number of ns on Daudi cells (Bmax) and and the assoiciation rate constant (ka) of chHHl (Dahle et al., 2007).

Materials and methods: chHHl was labeled with 1251 (example 3). 5 million cells per ml in 0.4 ml medium were suspended in tubes. One parallel of cells was blocked with 0.5 mg/ml of unlabeled chHHl for 15 minutes before adding 1251- Iabeled chHHl. One parallel of cells was not blocked. Both parallels were incubated with 100, 1000, 5000 and 10000 ng/ml of 125I-HH1. The cells were incubated for 5, 10, 20, 30 minutes and 1, 1.5 and 2 hours. After tion the cells were washed with PBS with 5 % foetal calf serum. The cells and the supernatant and washes were counted in a gamma counter. The experiment was ed three times.

ResultszTypical binding curves are shown in figure 2. The Kd of chHHl was 3.0 :hO.3, the Bmax of Daudi cells was 330000 d: 4000 CD37 ns per cell and the ka of chHHl was 0.36 i 0.03 nM/h. The data was similar as for murine HH1 (Kd = 2.7 i0.3, Bmax = 340000 i 5000 CD37 antigens per cell and ka 0.72 i 0.03 nM/h).

PCT/lBZOlZ/057230 Example 6 Biodistribution and tumor targeting in mice Biodistribution of labeled chimeric HH1 was studied in male nude BaIb/C mice.

Materials and methods: The radiolabeling was performed using p—SCN—Bn—DOTA as a bifunctional chelating agent to complex the radionuclide to the antibody (see Example 3). The preparation was stered by tail vein injection of 100 pl solution to each .

Male nude BaIb/C mice with a body weight of 21-25 g were used.

An activity of 120 kBq was injected in each animal. Five animals were used per time point. ies were performed after cervical dislocation at various time points after injection. The weight of each tissue sample was determined, and 111In were measured by a calibrated gamma or (Cobra II auto-gamma detector, Packard Instrument Company, Meriden, CT, USA). Samples of the injectates were used as references in the measurement ures.

Results: The biodistribution of 177Lu—chimeric HH1 at 1 hour, 20 hours and six days after injection is ted in Table 1. The data shows that the antibody has a relevant biodistribution similar to that observed with radiolabeled rituximab (Dahle et al, 2006 Nucl Med Biol, 33, 271—279). In conclusion, biodistribution of radiolabeled chimeric HH1 indicate that the antibody has relevant properties for in vivo use.

Table 1 Biodistribution of 177Lu—Iabeled chimeric HH1 in male nude Balb/C mice as 3O percent of injected dose per gram i SD.

Tissue 1 h 20 h 6 days Blood 25.0 i 2.9 15.2 :i: 1.0 8.9 :l: 3.5 Lung 7.0 i 1.1 5.2 :i: 0.9 2.9 i 0.7 Heart 7.8 :i: 0.8 5.8 2‘: 1.5 3.0 i 1.0 Liver 10.8 i 2.3 4.9 i 0.1 3.9 i 0.2 PCT/IBZOIZ/057230 Spleen 9.6 i 3.4 4.7 :l: 0.2 4.8 :t 1.1 Kidney 8.0 i 1.6 5.0 i 0.4 3.3 d: 1.0 Femur 4.5 :l: 2.4 2.8 :l: 0.6 1.7 i 0.3 Stomach 1.3 i: 0.5 1.0 :t 0.3 1.0 i 0.5 Small intestine 3.1 :t 0.7 1.7 :l: 0.1 1.3 i 0.3 Large intestine 1.4 i 0.3 1.4 i 0.2 0.9 i 0.2 Brain 0.7 i 0.1 0.3 d: 0.3 0.3 i 0.1 Example 7 In vitro ADCC assay using flow cytometry viability measurements Background: The ADCC property of chimeric HH1 antibody was evaluated using Natural killer (NK) cells and Daudi ma cells.

Methods: Human blood was harvested from volunteers and treated with Lymphoprep and Dynabeads to obtained isolated NK cells. The Daudi cells were treated with DiOC18 to stain the cells.

Daudi target cells were labeled with 10pL of DiOC18 per 106 target cells/mL by incubating for for 30 minutes at 370C. Cells were washed two times with PBS and re-suspend to 1 ml in cell culture medium.

The DiOC18 d Daudi Cells were labeled with dy by adding either chimeric HH1 or as a comparison rituximab to a concentration of 10 pg/ml and incubated for 15 minutes on ice before centrifugation and resuspension to remove unbound antibody.

Various amounts of NK cells were diluted to yield concentrations of 4x105 /ml (40:1), 2x105/ml (20:1), 1x105/ml (10:1) and 5x104/ml (5:1) in cell culture 3O medium. Propidium Iodide counterstain was ed by adding 40 pL of PI/ml of culture medium to make counter-stain solution.

Labeled Daudi cells were dilluted to 104 /ml in culture medium. The assay was started by mixing 50 pL of ors and 50 uL of Daudi cells into a reaction tube.

ZOIZ/057230 To the reaction tube was added SOuL of PI solution. After two hours of incubation at 37°C the percent of surviving Daudi cells were evaluated using flow cytometry.

For chHHl 11 % of the Daudi cells died by ADCC, while for rituximab 15 % of the cells died by ADCC. The percentage induction of ADCC was not significantly different for the two antibodies (see figure 3).

Example 8 In vitro ADCC cell assay using interleukin stimulated PBMC Background: ADCC activity of murine and chimeric versions of mAb HH1 was evaluated by by CrSl release assay.

Materials and Methods: The y of murine HH1 and chimeric HH1 to mediate antibody—dependent ediated cytotoxicity (ADCC) was assessed using Daudi cells as target cells and IL2—stimulated human PBMCs as effector cells.

Daudi cells (Human Burkitt's lymphoma cell line) were grown in tissue culture flasks (175 cm 2 ) using RPMI-1640, supplemented with 10% heat-inactivated fetal bovine serum, 1 mM sodium pyruvat, 2 mM L-glutamine and 1% Pen/Strep.

The cultures were maintained at a cell concentration n 3x105 and 1.5x106/ml by sub-cultivation with fresh culture medium 2—3 times a week. An aliquot of the cell e at a cell y between 0.5x106/ml and 1.0 x106/ml is centrifuged (1200 rpm) for 5 min.

Cells were washed once with washing buffer (DPBS with 2 % FCS) and pelleted (1200 rpm; 5 min). Cell pellet was resuspended in assay medium [RPMI w/HEPES, % FCS] and cell count was determined. Cell concentration was adjusted to 1x106/ml for labeling with chromium-51.

Approximately 30—50 ml whole blood drawn on EDTA glass from healthy donors was used for the isolation of PBMC. Whole blood was diluted 1: 1 with DPBS (Hanks' Balanced Salt Solution w/o calcium and magnesium) in a 50 ml tube.

Diluted whole blood was layered on top of Lymphoprep or) in a ratio 2:1 in a 50 ml tube and centrifuged at 1000><g for 20 min with slow braking. The PCT/I82012/057230 mononuclear cells from the interface were aspirated and washed twice with DPBS (300xg, 10 min).

The pelleted cells were gently resuspended in culture medium (RPMI—1640 with 10% heat-inactivated fetal bovine serum, 1% Pen/Strep) using a pipette and the cell count was determined in the cell counter.

The PBMC concentration was adjusted to ml and maintained in culture medium supplemented with 25ng/ml IL-2 (eBiosciences) and were cultured in 6 wells culture dish at 37° C in CO 2 incubator for 3 days. IL—2 stimulated PBMC were collected, d and resuspended in assay medium [RPMI w/HEPES, 10% FCS] at a concentration of 1.5x 106/ml. 1><106 Daudi cells were labeled with 3,7 MBq chromium—51 (CrSl) in a volume of 1 ml at 37° C for 1 hour.

The d cells were washed in DPBS with 2 % FCS three times by centrifugation (2000 rpm at 5 min).

The labeled cells were aliquoted in equal volumes for g of murine and chimeric HH1 including a control with no dy present. All samples were incubated at 37° C for 10 min, and then washed twice in DPBS with 2 % FCS three times by centrifugation (2000 rpm at 5 min).

The co—cultivation of effector cells with target cells was performed in triplicates in l round-bottom microtiter plates in a final volume of 200 pl.

First 10.000 target cells in a volume of 100 pl in assay medium were plated, followed by effector cells in a volume of 100 pl in at different concentrations to achieve the effector: target ratios assayed.

As a control, target cells were cultivated either in assay medium alone (spontaneous lysis) or in assay medium mented with 1% Triton X-100 (maximal lysis).

PCT/[32012/057230 The co-culture was incubated at 37° C. in a humid CO 2 incubator for 4 hours. The cytotoxic effect was measured by Cr51 release into the supernatant. At the end of the incubation cells were d from the culture medium by centrifugation (1500 rpm; 5 min) at room temperature. Cell free atants (100 ul/well) were transferred into 96 corresponding 0.2 ml micro tubes. The amount of Cr51 release was measured by ng in a Packard Cobra II Gamma counter.

Results: The results of the experiment are ted in Table 2. As shown the cell lysis of Daudi cells pretreated with the chimeric HH1 was significant for all three blood donors.

Pretreatment with the murine HH1 did not seem to cause any significant lysis compared to Daudi cells not pretreated with antibody.

Table 2. In vitro cell lysis after treatment with interleukin stimulated PBMC effector cells versus Daudi lymphoma target cells with pretreatment with anti— CD37 antibody Effector to target ratio Chimeric HH1 (% lysis) Murine HH1 (% lysis) Mean Range Mean Range 40: 1 159.2% 208.9% 106.5% 99.4-111.8% :1 177.9% 133.8—235.5% 105.5% 100.7—111.3% Effector cells from three individuals. Data were normalized to the measured cell lysis without antibody which was chosen as 100%.

Conclusion: When assayed using human PBMC in vitro, chimeric HH1 causes a icant ADCC in terms of cell lysis on lymphoma cells in vitro while murine HH1 did not affect any significant cell lysis effect compared to no treatment.

Example 9 g comparison between chimeric and murine HH1 To evaluate whether the chimeric HH1 had retained the antigen and epitope binding capability cells were saturated with chimeric and murine HH1 and then the ability to block the binding of radiolabeled HH1 was assessed. als and methods: A cell suspension of Daudi cells was distributed among 6 tubes with 1.7 million cells in 0.2 ml PBS in each tube. Tubes 1 and 2 were kept unblocked, tubes 3 and 4 were blocked by adding 3 ug of HH1 in 5 pi and tubes 5 and 6 were added 3 ug of chimeric HH1 in 5 ul.

The tubes were incubated for 10 minutes and fter added 2 ng of 1251 labeled HH1 and further incubated for 40 minutes at room temperature using a cell shaker.

The total activity in each tube was ed using a LKB gamma counter and thereafter the cells were washed three times with 1 ml PBS with 0.5% BSA and 1 mM DTPA. The final pellets were counted for radioactivity to determine the cell bound activity.

Results: It was found that the non—blocked cells retained 42.7% of the activity (range 42.7%-42.7%). Cells d with HH1 retained 1.6% of the activity (range 1.4%-1.8%). Cells blocked with chimeric HH1 retained 1.3% of the activity (range 1.1%-1.5%).

In conclusion, the binding of radiolabeled murine HH1 is blocked highly effective with chimeric HH1 and equal to that of blocking with murine HH1 indicating that the antigen and epitope g ability is maintained for the chimeric version of HH1.

This also indicate that the chimeric HH1 could be useful as pretreatment for blocking CD37 antigen in normal tissues prior to therapy with radioimmunoconjugate or oxin versions of HH1. 3O Example 10: ADCC for chHH1.1 with Rec-1 cells as target cells Materials and methods: Blood was drawn from three y individuals into EDTA glasses and used for peripheral blood mononuclear cells (PBMC) isolation. Whole blood was diluted 1:1 with DPBS (DPBS, Hyclone, Thermo ific, USA) in a 50 ml tube. Diluted whole blood was d on top of Lymphoprep (Medinor, Norway) in a ratio 2:1 in a 50 ml tube and centrifuged at 1000xg for 20 min with slow braking.

The mononuclear cells from the interface were aspirated and washed twice with DPBS (300xg, 10 min). The pelleted cells were gently resuspended in culture medium (RPMI-1640 with 10% heat-inactivated fetal bovine serum) using a pipette and the cell count was determined in the cell counter.

PBMC were stimulated with 25 ng/ml human recombinant IL-2 (eBiosciences) for 3 days.

Rec—1 mantle cell lymphoma cells (LG Standards, Boras, Sweden) were used as target cells in the xity assay. They were grown in tissue culture flasks (175 cm2) using RPMI-164O ne, Thermo Scientific, USA), supplemented with 10 % heat-inactivated fetal bovine serum (PAA, Thermo Scientific, USA).

Cell pellet was resuspended in assay medium (RPMI, 10% FCS) and cell count determined. Cell concentration was adjusted to 5><106 /ml for labeling with chromium-51. 5x106 cells were labeled with 3,7 MBq chromium—51 (51Cr) (PerkinElmer, lands) in a volume of 1 ml at 37° C for 1 hour. The labeled cells were washed three times in DPBS with 2 % FCS three times by centrifugation (2000 rpm at 5 min).

The labeled cells were aliquoted in equal volumes for binding of 20 ug/ml murine HHl, 20 ug/ml rituximab, 20 ug/ml chHH1.1 (IgGl isotype) or a ation of ug/ml mab and 20 ug/ml chHH1.1 including a control with no antibody present. All samples were incubated at 37° C for 10 min, and then washed twice in DPBS with 2 % FCS three times by centrifugation (2000 rpm at 5 min).

The effector cells were cultivated with target cells in a ratio of 40:1, 20:1 or 10:1 in l round-bottom microtiter plates in a final volume of 200 pl. First 10.000 target cells in a volume of 100 pl in assay medium are plated, followed by human PBMC in a volume of 100 pl. As controls, target cells were cultivated in assay PCT/IBZOlZ/057230 medium alone (spontaneous lysis) and in assay medium mented with 1% Triton X-100 (maximal lysis; total release).

All samples were run in triplicates. The co—culture was incubated at 37° C in a humid CO 2 incubator for 4 hours. The cytotoxic effect was measured by 51Cr release into the supernatant. At the end of the incubation cells were removed from the culture medium by centrifugation (1500 rpm; 5 min) at room temperature. Cell free supernatants (150 uI/well) were transferred into 96 corresponding 0.2 ml micro tubes. The amount of 51Cr released was measured by a gamma r (Cobra II, Packard, land). o/o specific lysis was calculated with the following equation: 100 x (experimental release — spontaneous release) /(total release — neous release).

Experimental release being the mean value of the replicates of a sample treatment. Triplicate samples for spontane release and total release were run for each dy treatment group, and the mean values were used for the experimental samples of the respective dy treatment.

Results: Figure 4 show that chHH1.1 was equally good as mab and slightly better than the murine HH1 in inducing specific lysis by ADCC in Rec-1 cells. The combination of chHHl and rituximab gave a slightly higher specific lysis than treatment with the two antibodies alone.

Conclusion: ADCC is an active mechanism of action for chHH1.1 in Rec-1 cells. Combination treatment with rituximab and chHH1.1 gave slightly higher ADCC than either antibody alone. 3O Example 11: CDC for 1 with Rec-1 cells as target cells Materials and s: Blood was drawn from three healthy individuals and serum was isolated by centrifugation.

Rec—1 mantle cell lymphoma cells (LG Standards, Boras, Sweden) were used as target cells in the cytotoxity assay. They were grown in tissue culture flasks (175 cm2) using RPMI—1640 ne, Thermo Scientific, USA), mented with 10 % heat-inactivated fetal bovine serum (PAA, Thermo ific, USA).

Cell pellet was resuspended in assay medium (RPMI, 10% FCS) and cell count determined. Cell tration was ed to 5x106/ml for labeling with chromium—51. 5x106 cells were labeled with 3, 7 MBq chromium-51 (51Cr) (PerkinElmer, Netherlands) in a volume of 1 ml at 37° C for 1 hour. The labeled cells were washed three times in DPBS with 2 % FCS three times by centrifugation (2000 rpm at 5 min). The labeled cells were aliquoted in equal volumes for binding of 20 ug/ml murine HH1, 20 ug/ml rituximab, 20 ug/ml chHH1.1 (IgGl isotype) or a combination of 20 ug/ml rituximab and 20 ug/ml chHH1.1 including a control with no antibody present. All samples were ted at 37° C for 10 min, and then washed twice in DPBS with 2 % FCS three times by centrifugation (2000 rpm at 5 min).

The target cells were cultivated with 10 % serum in 96-well round—bottom microtiter plates in a final volume of 200 pl. As controls, target cells were cultivated in assay medium alone (spontaneous lysis) and in assay medium supplemented with 1% Triton X-100 (maximal lysis; total release).

All samples were run in triplicates. The co-culture was incubated at 37° C in a humid CO 2 incubator for 2 hours. The cytotoxic effect was measured by SlCr release into the supernatant. At the end of the incubation cells were removed from the culture medium by centrifugation (1500 rpm; 5 min) at room temperature. Cell free supernatants (150 ul/well) were transferred into 96 3O corresponding 0.2 ml micro tubes. The amount of 51Cr released was measured by a gamma r (Cobra II, Packard, land). % specific lysis was calculated with the following on: 100 x (experimental release — spontaneous release) /( totalt release — spontaneous release).

Experimental release being the mean value of the replicates of a sample treatment. Triplicate s for spontane release and total release were run for each antibody treatment group, and the mean values were used for the experimental samples of the respective antibody treatment.

Results: Figure 5 shows that chHH1.1 do not work via a CDC ism alone, but chHH1.1 and mab together had a larger effect on specific lysis than the added effect of rituximab and chHH1.1 alone.

Conclusion: CDC is not an active mechanism of action for chHH1.1 alone. There was, however, a synergistic effect of atment with chHH1.1 and rituximab.

Example 12: Binding of 125I-chHH1.3 to Ramos cells Materials and methods: Chimeric HH1 with isotype IgG3 (chHH1.3) and murine HH1 (mHHl) were radiolabelled with 1251 using ct iodination method according to method supplied by manufacturer of iodination tubes (Pierce, UK). In one experiment Ramos cells were blocked with 20 mg 3 or mHHl and radiolabelled with 125I-chHH1.3 in order to measure non-specific binding.

In another ment Ramos cells were blocked with 20 mg ch HH1.3, mHHl or chHH1.1 and subsequently radiolabelled with 125I-mHHl to e non—specific binding. Binding to unblocked cells were used in both experiments to measure total binding.

DNA chHH1.3 Fc sequence with mutation full length heavy chain can be seen as SEQ ID NO: 10.

Amino acid chHH1.3 Fc sequence with mutation full length light chain can be seen as SEQ ID NO:11.

DNA chHH1.3 Fc sequence with mutation full length heavy chain can be seen as SEQ ID NO: 12.

PCT/132012/057230 Amino acid 3 Fc sequence with on full length light chain can be seen as SEQ ID NO:13. chHH1.3 Fc sequence without mutation (constant region) can be seen as SEQ ID NO: 14.

Results: When unblocked Ramos cells were radiolabeld with 125I-chHH1.3 the total binding was 81 % and when Ramos cells were radiolabeled with 125I-mHH1 the total binding was 77 °/o. However, when cells blocked with chHH1.3 or mHH1 were abeled with 125I—chHH1.3 the non-specific binding was 0.8 % and 44 % respectively, indicating that the chHH1.3 has higher affinity than mHH1 for the CD37 antigen. Furthermore, when cells blocked with chHH1.3, mHH1 or chHH1.1 were radiolabeled with 125I—mHHl the non-specifc binding was 0.3 %, 0.5 % and 0.7 %, respectively, indicating that the three antibodies bind to the same e on the CD37 antigen.

Table 3. Binding of 125I-chHH1.3 and 125I—mHH1 to Ramos cells.

RIC Non-blocked Blocked with 20 pg/ml cold antibody mHH1 chHH1.1 chHH1.3 1251-chHH1.3 81 % 44 % Not done 0.8 % 125I—mHHl 77 % 0.5 % 0.7 % 0.3 % Conclusion: The results ted that mHH1, chHH1.1 and chHH1.3 bind to the same epitope of CD37. ctedly, the affinity of 3 was higher than for mHH1.

Example 13: Biodistribution of 125I—chHH1.3 in nude mice Materials and methods: Chimeric HHl with isotype IgG3 (chHH1.3) was radiolabelled with 1251 using indirect iodination method according to method supplied by manufacturer of iodination tubes (Pierce, UK).

The ations were administered by tail vein injection of 100 pl solution to each animal. An activity of 600 kBq was injected per mice. Two s were used per time point. Autopsies were performed after cervical dislocation 24 hours and 48 hours after ion. The weight of each tissue sample was determined, and the activity of 1251 in each organ sample was measured by a calibrated gamma detector (Cobra 11 auto—gamma detector, Packard Instrument Company, Meriden, CT, USA). Samples of the injectates were used as references in the measurement ures. The decay corrected tages of the injected dose per gram tissue (o/oID/g) was calculated for each time point.

Results and conclusion: The chHH1.3 antibody showed a relevant distribution in nude mice (Figure 6).

Figure 6. Biodistribution (% I.D./g) of 125I—chHH1.3 in female nude mice 24 and 48 hours after ion.

Example 14: ADCC and CDC activity of chHH1.3 on Daudi cells Materials and methods: ADCC and CDC experiments were performed using the same procedures as described in Example 10 and 11, except that a commercially ble serum was used for the CDC assay and that the PBMC were stimulated over night with 25 ng/ml human recombinant IL—2 (eBiosciences) before ion of natural killer cells using the same assay as described in Example 7. For isolation of PBMC, blood was drawn from two individuals into EDTA tubes.

Daudi lymphoma cells (LG standards, Boras, Sweden) (target cells) were used as target cells in the cytotoxity assay. They were grown in tissue culture flasks (175 cm2) using RPMI—1640 (Hyclone, Thermo Scientific, USA), supplemented with 10 3O % heat—inactivated fetal bovine serum (PAA, Thermo Scientific, USA).

Cell pellet was resuspended in assay medium (RPMI, 10% FCS) and cell count determined. Cell concentration was adjusted to 5x106/ml for labeling with chromium-51.

PCT/[82012/057230 5x106 Daudi cells were labeled with 4 MBq chromium—51 (51Cr) (PerkinElmer, Netherlands) in a volume of 1 ml at 37° C for 1 hour. The labeled cells were washed three times in DPBS with 2 % FCS three times by centrifugation (2000 rpm at 5 min). The labeled cells were aliquoted in equal volumes for g of 20 mg/ml chHH1.3 dy or a combination of chHH1.3 and rituximab, both 20 mg/ml, and a control without antibody. All s were incubated at 37° C for min, and then washed twice in DPBS with 2 % FCS three times by centrifugation (2000 rpm at 5 min).

For the CDC assay the Daudi target cells were ated with 10 and 30 % serum in 96—well round-bottom microtiter plates in a final volume of 200 pl. For ADCC the NK effector cells were cultivated with Daudi target cells in a ratio of 10:1 (individual 1) or 15:1 (individual 2) in 96—well round—bottom microtiter plates in a final volume of 200 pl.

As controls, target cells were cultivated in assay medium alone (spontaneous lysis) and in assay medium supplemented with 1% Triton X-100 (maximal lysis; total release).

All samples were run in cates. The co-culture was incubated at 37° C in a humid C02 incubator for 2 hours. The cytotoxic effect was measured by 51Cr release into the supernatant. At the end of the incubation cells were removed from the e medium by centrifugation (1500 rpm; 5 min) at room temperature. Cell free atants (150 ul/well) were transferred into 96 corresponding 0.2 ml micro tubes. The amount of 51Cr released was measured by a gamma counter (Cobra II, Packard, land). % specific lysis was calculated with the following equation: 100 x (experimental e — spontaneous release) /( totalt release — spontaneous release).

Experimental release being the mean value of the replicates of a sample treatment. Triplicate samples for spontane release and total release were run for each antibody treatment group, and the mean values were used for the experimental samples of the respective antibody treatment.

Results: The chHH1.3 antibody did not induce specific lysis in Daudi B-lymphoma cells by the CDC ism. There was, however, a clear effect of the chHH1.3 antibody on specific lysis in Daudi ceiis by the ADCC mechanism (Figure 7).

Conclusion: CDC is not an active mechanism while ADCC is an active mechanism for chHH1.3 with Daudi cells as target.

Claims (21)

What is claimed is

1. An antibody molecule that binds to human CD37 and that is a chimeric antibody wherein the variable sequences are: i) a variable heavy chain comprising the amino acid sequence shown in SEQ ID NO: 1; and ii) a variable light chain comprising the amino acid sequence shown in SEQ ID NO :3.

2. The antibody molecule of claim 1 that is a ic antibody defined by 10 i) a le heavy chain comprising the amino acid sequence shown in SEQ ID NO: 1; ii) a le light chain comprising the amino acid sequence shown in SEQ ID NO:3, iii) constant heavy and light chains that are of human origin.

3. The antibody of claim 2, wherein i) the constant heavy chain is selected from the group consisting of 1961, IgGZ, IgG3 and IgG4 chain, and ii) the constant light chain is a kappa or a lambda chain.

4. The antibody of claim 3, wherein said constant heavy chain i) comprises the amino acid sequence encoded by SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 and/or shown in SEQ ID NO:11 and wherein said constant light chain ii) ses the amino acid sequence encoded by SEQ ID NO:9 or shown in SEQ ID 25 NO:13.

5. A DNA molecule comprising a region encoding the variable heavy chain of an antibody of any one of claims 1-4. 3O

6. The DNA molecule of claim 5, wherein said le heavy chain encoding region is fused to a region encoding a constant heavy chain of human , and wherein said human constant heavy chain is selected from the group consisting of IgGl, 1962, 1963 and IgG4.

7. The DNA molecule of claim 6, wherein said 1961 is encoded by a sequence shown in SEQ ID NO:5, SEQ ID NO:6 and/or SEQ ID NO:7 or said 1963 is encoded by a ce shown in SEQ ID NO:10 and/or SEQ ID NO:12.

8. A DNA molecule comprising a region encoding the variable light chain of an antibody of any one of claims 1—5.

9. An expression vector comprising a DNA molecule of claim 7 and/or a DNA molecule of claim 8.

10 10. A host cell carrying one or more vectors of claim 9, provided that if the cell is a human cell, it is eX vivo.

11. A method for producing an antibody of any one of claims 1 to 4, sing transfecting a ian host cell with one or more vectors of claim 9, culturing 15 the host cell and recovering and purifying the antibody molecule, ed that if the cell is a human cell, it is ex Wm.

12. A pharmaceutical composition comprising, as the active ingredient, one or more anti-CD37 antibody molecules of any one of claims 1 to 4, and a 20 pharmaceutically acceptable carrier.

13. Use of an dy molecule of any one of claims 1 to 4 or a pharmaceutical composition containing such antibody molecule according to claim 12 in the manufacture of a medicament for depleting CD37 expressing B—cells from a 25 population of cells.

14. A radioimmunoconjugate that binds human CD37 comprising: a) an antibody according to any one of claims 1—4, b) a linker, and 30 c) a radionuclide selected from the group ting of 211At, 213Bi, 212Bi, lepb, 225AC, 227Th, 90Y, 186Re, 188Re, 199AU, 194Ir, 166HO, lngd, 153Sm, 149Pm, 142Pl‘, lllAg, lOgPd, 77AS, 67CU, 475C, and 177LU.

15. A pharmaceutical composition comprising a radioimmunoconjugate according 35 to claim 14, and a pharmaceutically acceptable carrier.

16. The pharmaceutical composition according to claim 12 or 15, for treating B- cell malignancies.

17. Use of a pharmaceutical composition according to claim 12 or 15 in the manufacture of a medicament for treatment of a B-cell malignancy selected from the group consisiting of B-cell non~Hodgkins lymphoma, B—cell chronic lymphocytic leukemia, hairy cell leukemia, lymphoplasmacytic ma and multiple myeloma.

18. A kit for the production of the radioimmunoconjugate according to of claim 14 comprising two or more vials, wherein one vial contains a conjugate comprising a chelator linked to an antibody according to any one of claims 1—4; and a second vial contains a radionuclide.

19. An injectable preparation of chimeric or zed HHl dy for use in pretreatment of a B—cell malignancy, n CD37 is blocked in normal tissues before radioimmunotherapy with radiolabeled or immunotoxic versions of HH1.

20 20. The preparation according to claim 19, wherein the amount of HHl antibody is at least 0.5 mg and not more than 1 g of antibody.

21. The dy of any one of claims 14, substantially as herein described with reference to any one of the es and/or