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NZ736027B2 - Methods of treating cancer harboring hemizygous loss of tp53 - Google Patents
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NZ736027B2 - Methods of treating cancer harboring hemizygous loss of tp53 - Google Patents

Methods of treating cancer harboring hemizygous loss of tp53 Download PDF

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Publication number
NZ736027B2
NZ736027B2 NZ736027A NZ73602716A NZ736027B2 NZ 736027 B2 NZ736027 B2 NZ 736027B2 NZ 736027 A NZ736027 A NZ 736027A NZ 73602716 A NZ73602716 A NZ 73602716A NZ 736027 B2 NZ736027 B2 NZ 736027B2
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New Zealand
Prior art keywords
polr2a
cancer
cells
gene
cell
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NZ736027A
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NZ736027A (en
Inventor
Yunhua Liu
Xiongbin Lu
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Board Of Regents The University Of Texas System
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Publication date
Application filed by Board Of Regents The University Of Texas System filed Critical Board Of Regents The University Of Texas System
Priority claimed from PCT/US2016/020687 external-priority patent/WO2016141185A1/en
Publication of NZ736027A publication Critical patent/NZ736027A/en
Publication of NZ736027B2 publication Critical patent/NZ736027B2/en

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    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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Abstract

Provided herein are methods of treating a patient having a cancer that exhibits (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a decreased level of expression of a POLR2A gene product relative to a reference (i.e., control) expression level. The methods comprise administering a therapeutically effective amount of a POLR2A inhibitor (e.g., a nucleic acid that inhibits the expression of a POLR2A protein, an amatoxin, alpha-amanitin, or alpha-amanitin conjugated to a cell targeting moiety, such as an EpCAM antibody) to a patient having or determined to have (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a decreased level of expression of a POLR2A gene product relative to a reference (i.e., control) level.

Description

DESCRIPTION METHODS OF TREATING CANCER HARBORING HEMIZYGOUS LOSS OF BACKGROUND OF THE INVENTION id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1" id="p-1"
[0001] This application claims benefit of priority to US ional ation Serial No. 62/128,480, filed March 4, 2015, the entire contents of which are hereby incorporated by nce The invention was made with government support under Grant Nos. R01 CA136549 and U54 CA151668 awarded by the National Institutes of Health. The government has certain rights in the invention. 1. Field of the Invention The present invention relates generally to the fields of medicine and cancer biology. More particularly, it concerns methods of treating cancers harboring hemizygous loss of TP53. 2. Description of Related Art TP53, a well-known tumor suppressor gene, is frequently inactivated by mutation or deletion in a majority of human tumors gean et al., 2007; Vazquez et al., 2008). A tremendous effort has been made to restore p53 activity in cancer therapies (Chene, 2003; Wade et al., 2013). Whereas gene therapy using adenoviral vectors expressing pe p53 has shown activity in l clinical trials, the le and insufficient gene delivery to every tumor cell and the presence of a host antibody to adenovirus d its clinical use (Lane et al., 2010; Haupt and Haupt, 2004). A number of small chemical compounds that boost p53 activity have also been developed. However, they can only be applied in human cancers possessing wildtype p53 (Cheok et al., 2011; Goldstein et al., 2011). However, no effective sed therapy has been successfully translated into clinical cancer ent due to the complexity of p53 regulators and their poor drugability. As such, new strategies to treat p53-deficient cancers are needed.
SUMMARY OF THE INVENTION In some embodiments, there are provided methods of ng a patient having cancer cells that exhibit (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a decreased level of expression of a POLR2A gene t relative to a reference expression level (e. g., an sion level in a non-cancerous sample), the method comprising administering a therapeutically effective amount of a POLR2A inhibitor to such a patient. In certain aspects, the patient has cancer cells that t a hemizygous loss of the TP53 gene. In certain aspects, the patient has cancer cells that exhibit a hemizygous loss of the POLR2A gene. In certain aspects, the patient has cancer cells that exhibit a decreased level of expression of a POLR2A gene product relative to a reference expression level.
In certain aspects, a POLR2A inhibitor comprises a c acid that inhibits the expression of a POLR2A protein. In certain s, a POLR2A inhibitor comprises alpha-amanitin. In some aspects, the alpha-amanitin is conjugated to an antibody, such as an antibody that targets a cancer cells (e. g., a tumor-associated antigen antibody). In some s, the antibody may be an EpCAM-specific antibody.
In certain aspects, the POLR2A gene product is an mRNA. A level of expression of an mRNA may be determined by Northern blotting, reverse transcriptionquantitative real-time PCR (RT-qPCR), nuclease protection, transcriptome analysis, a hybridization assay, a chip-based expression platform, or an invader RNA assay platform. In certain aspects, the POLR2A gene product is a protein. A level of expression of a n may be determined by mass spectrometry, western blot, ELISA, immunoprecipitation, immunohistochemistry, or radioimmunoassay. In certain s, a genomic copy number is detected to determine the hemizygous loss of the TP53 gene or the hemizygous loss of the POLR2A gene. In some aspects, genomic copy number is determined by a c hybridization technique (e.g. FISH analysis), PCR analysis (e. g., real-time PCR), or restriction fragment is.
In certain aspects, the cancer is a lung cancer, brain cancer, breast cancer, liver cancer, ovarian , colorectal , prostate cancer, or pancreatic cancer. In certain aspects, the cancer is metastatic, recurrent, or multi-drug resistant.
In certain aspects, the methods further comprise administering at least a second anticancer therapy to the subject. In certain aspects, the second anticancer therapy is a surgical therapy, chemotherapy, radiation therapy, cryotherapy, hormonal therapy, toxin therapy, immunotherapy, or cytokine therapy. In certain aspects, the chemotherapy is 5- ?uorouracil, oxaliplatin, or SN-38.
In certain aspects, the t is a human. In certain aspects, the patient is a non-human mammal.
In certain aspects, the patient is treated at least a second time. In certain aspects, the patient is treated over a period of 1 week to 6 months. In certain s, the patient has previously undergone at least one round of anti-cancer therapy In some embodiments, there are provided methods of ng a patient having cancer comprising (a) selecting a patient determined to have cancer cells comprising (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a sed level of expression of a POLR2A gene product relative to a reference level; and (b) administering a therapeutically effective amount of a POLR2A inhibitor to the patient.
In certain aspects, selecting a patient ses obtaining a sample of the cancer and determining r cells of the cancer comprise (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a decreased level of expression of a POLR2A gene product relative to a reference level. In certain aspects, the methods further se providing a report of the determining. In certain aspects, the report is a written or electronic report. In certain aspects, the report is provided to the patient, a health care payer, a physician, an insurance agent, or an onic system.
In certain s, ing a patient comprises ing results for a test that determines whether the cells of the cancer comprise (i) a gous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a decreased level of expression of a POLR2A gene product relative to a reference level.
In certain aspects, the cells of the cancer comprise a hemizygous loss of the TP53 gene. In certain aspects, the cells of the cancer comprise a hemizygous loss of the POLR2A gene. In certain s, the cells of the cancer comprise a decreased level of sion of a POLR2A gene product ve to a reference level (e. g., an expression level in a non-cancerous sample).
In some embodiments, there are provided methods of selecting a drug therapy for a cancer patient comprising (a) obtaining a sample of the cancer; (b) ing the presence of (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; and/or (iii) a decreased level of expression of a POLR2A gene product relative to a reference level in the cells of the cancer; and (c) selecting a POLR2A tor if (i) a hemizygous loss of the TP53 gene is detected; (ii) a hemizygous loss of the POLR2A gene is detected; and/or (iii) a decreased level of expression of a POLR2A gene product relative to a reference level is detected in the cells of the cancer.
In certain aspects, the methods further se stering a therapeutically effective amount of a POLR2A inhibitor to the patient.
In certain aspects, the cells of the cancer comprise a hemizygous loss of the TP53 gene. In certain aspects, the cells of the cancer comprise a hemizygous loss of the POLR2A gene. In certain aspects, the cells of the cancer comprise a decreased level of expression of a POLR2A gene product relative to a reference level (e. g., an expression level in a non-cancerous sample).
In some embodiments, compositions are provided comprising a POLR2A inhibitor for use in the ent of a cancer in a subject, wherein cells of the cancer have been determined to comprise (i) a hemizygous loss of the TP53 gene; (ii) a gous loss of the POLR2A gene; or (iii) a decreased level of expression of a POLR2A gene product relative to a reference level (e. g., an expression level in cells of a non-cancerous sample).
In some embodiments, there is provided the use of a POLR2A inhibitor in the cture of a medicament for the treatment of a cancer, wherein cells of the cancer have been determined to comprise (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; or (iii) a decreased level of expression of a POLR2A gene product relative to a reference level (e. g., an expression level in cells of a non-cancerous sample).
As used herein, "essentially free," in terms of a specified component, is used herein to mean that none of the specified component has been efully formulated into a composition and/or is present only as a inant or in trace amounts. The total amount of the specified component resulting from any unintended contamination of a composition is therefore well below 0.05%, preferably below 0.01%. Most preferred is a composition in which no amount of the specified component can be detected with standard ical methods.
As used herein the specification, "a" or "an" may mean one or more. As used herein in the claim(s), when used in conjunction with the word "comprising," the words "a" or "an" may mean one or more than one.
The use of the term "or" in the claims is used to mean "and/or" unless explicitly indicated to refer to alternatives only or the alternatives are ly exclusive, although the disclosure supports a definition that refers to only alternatives and "and/or." As used herein "another" may mean at least a second or more.
Throughout this application, the term "about" is used to te that a value includes the inherent variation of error for the device, the method being employed to determine the value, or the variation that exists among the study subjects. id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25" id="p-25"
[0025] Other objects, features and advantages of the present invention will become nt from the following detailed description. It should be understood, however, that the detailed description and the specific examples, while indicating preferred embodiments of the invention, are given by way of ration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description.
BRIEF DESCRIPTION OF THE DRAWINGS The following drawings form part of the present specification and are included to further trate certain aspects of the present invention. The invention may be better understood by reference to one or more of these drawings in ation with the detailed description of specific embodiments presented herein.
FIGS. 1A-H. Expression of POLR2A, but not TP53, is correlated with the gene copy number. () Analysis of TCGA se shows the frequencies of hemizygous deletion of the TP53 locus in a variety of human cancers. () Schematic m of genes adjacent to TP53 in human genome. () Concomitant deletion of POLR2A in colorectal tumors harbouring hemizygous loss of TP53. () Scatterplots of POLR2A copy number versus mRNA expression for clinical colorectal tumors in TCGA and cancer cell lines in CCLE. A linear regression and the Pearson correlation coefficient (r) are displayed. () Quantification of POLR2A protein levels in d normal and CRC tissue samples (neutral or hemizygous-loss ). () Copy number variations of POLR2A and TP53 in human CRC cell lines. Left columns are TP53; right columns are POLR2A. () Relative expression levels of POLR2A in CRC cell lines (normalized to HCT116). () Protein levels of POLR2A, p53 and B-Actin in CRC cell lines.
FIGS. 2A-J. POLR2Al°SS cells are highly sensitive to the POLR2A inhibition. () POLR2Aloss cells (SW837, SNU283) are significantly more sensitive to (x-Amanitin treatment than POLRZAHC'ltral cells (HCT116, SW480). Crystal violet staining of cells is shown. () Cell proliferation of POLRZAHC'ltral and POLR2Aloss cells with 0t- Amanitin treatment. () Dox-induced suppression of POLR2A inhibited the proliferation of SNU283 cells, but not of HCTll6 cells. () ation between POLR2A mRNA expression and cell proliferation in HCTll6 and SNU283 cells expressing ducible POLR2A shRNAs. Data represents mean i SD. Left columns are POLR2A mRNA; right columns are Proliferation. (FIGS. 2E and 2F) Survival curves of SUN283 and SW837 cells in response to increasing doses of (x-Amanitin treatment after ection with increasing amounts of PORLZA expression vector DNA. () POLR2Aloss HCTll6 cells are icantly more ive to (x-Amanitin treatment than the parental POLRZAHC'ltral HCTll6 cells. Crystal violet staining of cells is shown. () Cell proliferation of POLRZAHC'ltral and POLR2Aloss HCTll6 cells treated with (x-Amanitin. (FIGS. 21 and 2J) Correlation between POLR2A mRNA expression and cell proliferation (; left columns are POLR2A mRNA; right columns are Proliferation) or apoptosis (; left columns are sthrl; middle columns are shRNA-l; right columns are shRNA-2) in HC'ltral and POLR2Aloss HCTll6 cells. Data represents mean i SD.
FIGS. 3A-E. The sensitivity of POLR2Al°SS cells to POLR2A inhibition is independent of p53. () Protein levels of POLR2A, p53, EpCAM and B-Actin in a panel of ic human primary colorectal cancer xhCRC cell lines. () Growth curve of HC'ltral and loss xhCRC cells. () Cell proliferation of POLRZAHC'ltral and POLR2Aloss xhCRC cells treated with (x-Amanitin. () Sensitivity of POLRZAHC'ltral and POLR2Aloss xhCRC cells to 5-FU, Oxaliplatin (Oxa) or SN-38 treatment combined with or without (x-Amanitin ent. () Cell proliferation of POLR2AHC'ltral and POLR2Aloss xhCRC cells treated with ama-HEA125 (anti-EpCAM) antibody-drug conjugate at various concentrations.
FIGS 4A-E. Suppression of POLR2A selectively inhibits the POLR2Aloss tumor growth. () Growth curves of aft tumors derived from subcutaneously implanted HCT116 (1 x 106 cells) and SNU283 (2 x 106 cells) cells. Both cell lines express control or Dox-inducible POLR2A shRNAs. After initial establishment of tumors (100 mm3), mice were treated with 1 ug ml'1 of Dox in drinking water. 11 = 5 mice per group. (FIGS. 4B and 4C) Tumor growth curves () of aft tumors derived from orthotopically implanted POLR2AHC'ltral and POLR2Aloss HCT116 cells (1 x 106 cells injected) expressing Dox-inducible control or POLR2A shRNA. After initial establishment of , mice were treated with lug ml"1 Dox in drinking water. Tumor weights () were ed (11 = 5 mice per group). **p < 0.01. (FIGS. 4D and 4E) Tumor growth curves of xenografted tumors derived from orthotopically implanted POLR2AHC'ltral and POLR2Aloss HCT116 ( 4D; 1.0 x 106 cells injected) or xhCRC (; 0.5 x 106 cells ed) cells that received dual intraperitoneal injections of anti-EpCAM antibody (3.6 mg kg'l) or A125 antibody-drug conjugate (3, 10, 30 and 90 ug kg'l, corresponding to 0.12, 0.4, 1.2 and 3.6 mg IgG kg'l). n = 10 mice per group.
FIGS. SA-B. Expression of POLR2A correlates with its gene copy number in human colon tumors. Double-color FISH analysis was med using a probe for chromosome 17 centromere and locus-specific probe for POLR2A on a human colon tissue rray. Hemizygous loss of the POLR2A gene was determined and the results are shown in Table 2. () Quantification of POLR2A expression in human colon normal, POLR2A-neutral or -loss tumor tissue s. Error bars,s.d. () Protein levels of POLR2A and B-Actin in matched normal and CRC tissue samples.
FIGS. 6A-C. Expression of TP53 is not associated with its gene copy number. (FIGS. 6A and 6B) Scatterplots of TP53 copy number versus protein expression () or mRNA expression () in colorectal tumors in TCGA database. The n correlation coefficient (r) and p value are displayed. () Relative mRNA expression of TP53 in colorectal cancer cell lines (normalized to that in HCT116 cell line).
Data are mean and s.d. of three independent experiments.
FIGS. 7A-G. POLRZAIOSS cells are highly sensitive to POLR2A inhibition.
() Cell proliferation of POLRZAHC'ltral and POLR2Aloss cells treated with actinomycin D. () Knockdown efficiency of POLR2A-specific shRNAs in HCT116, SW480, SW837 and SNU283 cells. () Effect of POLR2A knockdown on the proliferation of four colorectal cancer cell lines. Cells expressing GFP and control or -specific shRNAs were sorted and mixed with control GFP-negative cells (1:1) and the GFP positive cells were quantified at e 2, 4 and 6. **p < 0.01, us: not icant. () Protein levels of POLR2A and B-Actin in HCT116 and SNU283 cells sing Dox-inducible POLR2A shRNAs (1.0 ug ml'1 Dox). () Cell proliferation of HCT116 and SNU283 cells expressing Dox-inducible POLR2A shRNA in the presence of 300 ng ml"1 Dox. **p < 0.01. (FIGS. 7F and 7G) Cell cycle profiles (; top portion of each column is G2/M; middle portion of each column is S; bottom portion of each column is G0/G1) and apoptosis () of control or POLR2A expressing HCT116 and SNU283 cells. ** 0.01. Data are mean and s.d. of three independent ments. id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34" id="p-34"
[0034] FIGS. 8A-B. Ectopic expression of POLR2A restores the resistance of POLRZAIOSS cells to a-Amanitin treatment. () Protein levels of POLR2A and B- Actin in SNU283 and SW837 cells expressing increasing amounts of exogenous POLR2A.
() Crystal violet staining of SNU283 and SW837 cells treated with increasing doses of (x-Amanitin after transfection with sing amounts of POLR2A expression vector DNA.
FIGS. 9A-H. Mono-allelic knockout of POLR2A sensitizes HCT116 cells to POLR2A inhibition. () Schematic illustration of the Cas9/ngNA-targeting sites in the POLR2A gene. Two ngNA-targeting sequences are shown (the arrow indicates ionality; sequences are provided as SEQ ID NOs: 25-26) and the protospacer—adjacent motif (PAM) ces are the last three nucleotides of each sequence. () Efficiency of the Cas9-mediated cleavage of POLR2A in HCT116 cells measured by the Surveyor assay.
() Sequences of mutant POLR2A alleles in the cells of colonies 14 and 5 (sequences are provided as SEQ ID NOs: 27-30). () Protein levels of POLR2A and B-Actin in POLRZAHC'ltral and loss HCT116 cells. () Growth curves of POLRZAHC'ltral and POLR2Aloss HCT116 cells. () Relative proliferation of POLRZAHC'ltral and POLR2Aloss cells treated with actinomycin D. () Effect of POLR2A knockdown on the POLRZAHC'ltral and POLR2Aloss HCT116 cells. Experiments were performed as described in . **p < 0.01, ns: not significant. () Dox-induced partial suppression of POLR2A inhibited the growth of POLR2Aloss HCT116 cells, but not of parental POLRZAHC'ltral HCT116 cells. Data are mean and s.d. of three independent experiments.
FIGS. 10A-G. Sensitivity of POLRZAIOSS cells to POLR2A inhibition is ndent of p53. (A) Schematic illustration of the Cas9/ngNA-targeting sites in the TP53 gene. Two ngNA-targeting sequences are shown (the arrow indicates directionality; sequences are provided as SEQ ID NOs: 31-32) and the PAM sequences are the last three nucleotides of each sequence. (B) Efficiency of the Cas9-mediated cleavage of TP53 in HCT116 cells measured by Surveyor assay. (C) Protein levels of , p53 and B-Actin in a panel of isogenic HCT116 cells. (D) Growth curves of POLRZAHC'ltral and POLR2Aloss HCT116 cells. (FIGS. 10E and 10F) Crystal staining images (E) and cell survival curves (F) of POLRZAHC'ltral and POLR2Aloss HCT116 cells after (x-Amanitin treatment. (G) Cell survival curves of POLRZAHC'ltral and POLR2Aloss HCT116 cells in response to the treatment of ama-HEA125. id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37" id="p-37"
[0037] FIGS. 11A-C. Dose-dependent suppression of POLR2A inhibits tumorigenesis in POLRZAIOSS, but not neutral tumors. (A) Quantification of POLR2A mRNA expression levels in subcutaneously xenografted HCT116 and SNU283 tumors expressing l or POLR2A shRNA (n = 5 mice per group). ** p < 0.01. (B) Cells positive for Ki67 (cell proliferation) or cleaved caspase-3 (apoptosis) per field and POLR2A expression by immunohistochemical staining were quantified. ** p < 0.01. Data are mean and s.d. (C) Quantification of tumor sizes of xenograft tumors derived from aneously implanted HC'ltral and POLR2Aloss HCT116 cells (1 x 106 cells injected). Both cell lines express control or Dox-inducible POLR2A shRNAs. After the initial establishment of tumors (100 m3), mice were treated with (0.5, 1, and 2 ug ml'l) Dox in drinking water. 11 = 5 mice per group. Data are mean and s.d.
FIGS. 12A-F. Suppression of POLR2A with DOPC-encapsulated POLR2A siRNA inhibits the growth of POLRZAIOSS tumors. (A) Western blots for POLR2A and B-Actin following transfection of control siRNA or POLR2A siRNAs (#1 and #2) in HCT116 cells. (B) tic ration of orthotopic injection of HCT116 cells (1 x 106 cells) followed by DOPC nanoliposome treatment intervals. (FIGS. 12C and 12D) Tumor growth curves of orthotopic aft tumors derived from POLRZAHC'ltral and POLR2Aloss HCT116 cells (C is siPolZ-l; D is siP012-2) that received intraperitoneal injections of l (1,000 ug kg'l) or POLR2A siRNAs (125, 250, 500 and 1,000 ug kg'l) twice weekly. n = 10 mice per group. (FIGS. 12E and 12F) Representative protein levels of POLR2A and n in aft tumors following control or POLR2A siRNA treatment (E is -1; F is siPol2-2).
FIGS. 13A-D Suppression of POLR2A selectively inhibits the POLR2Aloss tumor growth (FIGS. 13A AND 13B) Tumor weights of orthotopically implanted HCT116 (A) and XhCRC (B) tumors were measured (11 = 10 mice per group). (FIGS. 13C AND 13D) Body weights (C) and liver enzymes (D) including alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline atase in peripheral blood were recorded as described in Methods. Data shown are the means of five mice in each group.
FIGS. 14A-F. Suppression of POLR2A by ama-HEA125 inhibits the growth of POLR2Aloss tumors. (FIGS. 14A, 14C, and 14E) Protein levels of POLR2A and B-Actin in HCT116 (A), SW480 (C), or SW837 (E) cells. These cell lines are POLR2A-neutral, -loss, or POLR2A-restored. (FIGS. 14B, 14D, and 14F) Tumor growth curves of orthotopic xenograft tumors derived from the corresponding cells as indicated. All of them received dual eritoneal injections of pCAM antibody (3.6 mg kg'l) or A125 antibody-drug conjugate (10 and 90 ug kg'l, corresponding to 0.4 and 3.6 mg IgG kg'l). n = 10 mice per group.
DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS The studies provided herein demonstrate that genomic deletion of TP53 frequently encompasses neighbouring essential genes, rendering cancer cells with hemizygous TP53 deletion vulnerable to further suppression of such genes. POLR2A is identified as such an essential house-keeping gene that is virtually co-deleted with TP53 in many types of human cancer. It encodes the t and catalytic subunit of RNA polymerase II complex, which is specifically inhibited by (x-amanitin (Bensaude, 2011; Lindell et al., 1970). As such, d of pharmacologic intervention of p53 or its regulators, the principle of collateral vulnerability to POLR2A inhibition provides a brand-new strategy for cancer therapy. id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42" id="p-42"
[0042] The present analysis of clinical samples and cancer cell lines in The Cancer Genome Atlas (TCGA) and Cancer Cell Line Encyclopaedia (CCLE) reveals that POLR2A sion levels are y correlated with its gene copy numbers in human colorectal tumors. Suppression of POLR2A with (x-amanitin, small ering RNAs, or short-hairpin RNAs, selectively inhibited proliferation, survival, and tumorigenic ial of colorectal cancer cells with hemizygous TP53 loss in a dependent manner. Moreover, the present preclinical studies with (x-amanitin predict therapeutic efficacy of (x-amanitin-based drugs or inhibitors against POLR2A for treating colorectal cancer and it should be applicable to many types of human cancers with gous loss of TP53.
Previous clinical applications of (x-amanitin have been limited due to its liver toxicity (Letschert et al., 2006). Free (x-amanitin is toxic to liver because it is specifically bound by OATP1B3, a transporter exclusively expressed on the membrane of hepatocytes (Letschert et al., 2006). However, (x-amanitin, when conjugated with specific antibodies, is no longer a substrate for OATP1B3 (Letschert et al., 2006; Moldenhauer et al., 2012; Faulstich and Fiume, 1985). Here, it is shown that low doses of an (x-amanitin-antibody conjugate (e. g., (x-Amanitin-conjugated anti-EpCAM (Epithelial Cell Adhesion Molecule) antibody) lead to tumor regression in murine models of human colorectal cancer with hemizygous deletion of POLR2A. Inhibiting POLR2A is a novel therapeutic approach for cancers harbouring such common genomic alterations.
I. Cell-targeting Conjugates It may be desired to ate an nitin molecule (or any amatoxin) to at least one cell-targeting agent to enhance the utility of (x-amanitin and reduce liver toxicity.
Such a conjugate may be termed an "immunotoxin.73 For example, in order to increase the cy and utility of (x-amanitin as a therapeutic agent, it may be conjugated or covalently bound to a d cell targeting moiety. Such a moiety may be any moiety with sufficient selectivity, icity, or affinity for targeting a desired cell type by binding to an external receptor or binding site on said cell types, such as a cancer cell (US. Patent Publn. No. 2009/0304666). Examples of such es include, but are not limited to, antibodies or antigen-binding fragments thereof, antibody-like proteins, and aptamers. Examples of antigen-binding antibody fragments include without limitation: (i) the Fab nt, consisting of VL, VH, CL and CH1 domains; (ii) the "Ed" fragment consisting of the VH and CH1 s; (iii) the "EV" fragment consisting of the VL and VH domains of a single antibody; (iv) the "dAb" fragment, which consists of a VH domain; (v) isolated CDR regions; (vi) F(ab')2 fragments, a bivalent nt comprising two linked Fab fragments; (vii) single chain Fv molecules ("scFv"), wherein a VH domain and a VL domain are linked by a peptide linker that allows the two domains to associate to form a binding domain; (viii) bi-specific single chain Fv dimers (see U.S. Patent No. 5,091,513) and (ix) diabodies, multivalent or multispecific fragments constructed by gene fusion (U.S. Patent Publn. No. 2005/0214860).
Fv, scFv or diabody molecules may be stabilized by the incorporation of disulfide bridges linking the VH and VL domains. A cell targeting moiety may specifically bind to any tumor- associated antigen, such as, for example, CD19, CD20, CD30, CD33, CD52, EpCAM, carcinoembryonic antigen, alphafetoprotein, gpA33, Mucins, CA-125, MUC-1, CD56, EGFR, ERBB2, ERBB3, c-Met, IGFlR, EPHA3, TRAILRl, TRAILR2, RANKL, FAP, Tenascin, AKT, Her2/neu, Her3, epithelial tumor antigen, melanoma-associated antigen, d p53, mutated ras, Dectin-1, gp100, MART-l/MelanA, TRP-l (gp75), Tyrosinase, TAG-72, CAIX, PSMA, Folate-binding protein, gangliosides (e. g., GD2, GD3, GM2), MAGE-l, MAGE-3, BAGE, GAGE-1, GAGE-2, N-acetylglucosaminyltransferase-V, p15, B- catenin, MUM-1, CDK4, HPV-E6, HPV-E7, ZFP161, Ubiquilin-l, HOX-B6, IFI27, YB-1, KIAA0136, Osteonectin, F-box only n 21, ILF3, SSX-2, PSMA, NY—ESO-l, FRAME, mesothelin, VEGF, VEGFR, Integrin alphaVbeta3, Integrin alpha5beta1, or PLKl.
Several methods are known in the art for the attachment or conjugation of a cell targeting moiety (e. g., a associated antigen directed dy) to a conjugate (e. g., (x-amanitin) (see PCT Publn. WO2012/119787, which is incorporated herein by reference in its entirety). For example, an immunotoxin may employ a cleavable ide linker well known in the art. The toxin may be conjugated to the cell targeting moiety by treating the toxin to provide sulfhydryl groups and the cell targeting moiety to provide l disulfide residues. Other linkages that are commonly utilized and expected to be useful for conjugating a toxin to cell targeting moieties are imminothiolane/succinimidyl 4-(N-maleimidomethyl) cyclohexane- 1-carboxylate and carbodiimide es. Many other es for conjugating proteins of varying lengths, stability, ?exibility and chemical reactivity are well known in the art and in many cases are commercially available. Some es of attachment methods involve the use of a disulfide ge reaction; by forming a thioether bond; a metal chelate complex employing, for example, an organic chelating agent, such a diethylenetriaminepentaacetic acid anhydride (DTPA); netriaminetetraacetic acid; N- -p-toluenesulfonamide; and/or tetrachloro60t-diphenylglycouril-3 attached to the cell targeting moiety. Cell ing moieties may also be reacted with an enzyme in the presence of a ng agent such as glutaraldehyde or periodate. It is desirable that any (x-amanitin- cell targeting moiety conjugate be stable upon extended exposure to serum. As such, 0t- amanitin may be ated to a lysine residue of a cell targeting moiety by way of a stable urea linker that is serum-stable but will release free nitin inside of a targeted cell following lysosomal degradation of the cell targeting moiety. 11. Treatment of Disease Certain aspects of the present embodiments can be used to prevent or treat a disease or disorder associated with gous loss of TP53 and associated loss of POLR2A.
Functioning of POLR2A may be reduced by any suitable substances to treat a cancer harboring gous loss of TP53 and/or . Such exemplary substances can be any amatoxin, (x-amanitin, or cell-targeting moieties conjugated to any amatoxin or (x-amanitin.
A cancer harboring hemizygous loss of TP53 and/or POLR2A may not be homogenous with regard to the loss of TP53 and/or POLR2A. In various aspects, about 5%, %, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100% of the cells that comprise the cancer may harbor a gous loss of TP53 and/or . Thus, in some aspects, about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 99% of the cells that se the cancer may comprise both copies of TP53 and/or POLR2A. In other aspects, various percentages of cells comprising the cancer may harbor a homozygous loss of TP53 and a gous loss of POLR2A. id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48" id="p-48"
[0048] "Treatment" and "treating" refer to administration or application of a therapeutic agent to a subject or performance of a procedure or modality on a subject for the purpose of obtaining a therapeutic benefit of a disease or health-related condition. For example, a treatment may e administration of a pharmaceutically effective amount of an substance that inhibits the function POLR2A. id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49" id="p-49"
[0049] A "subject" refers to either a human or non-human, such as primates, mammals, and vertebrates. In particular embodiments, the subject is a human.
The term "therapeutic benefit" or "therapeutically effective" as used throughout this application refers to anything that promotes or enhances the well-being of the subject with respect to the medical treatment of this condition. This includes, but is not d to, a reduction in the frequency or ty of the signs or symptoms of a disease. For example, treatment of cancer may involve, for example, a reduction in the size of a tumor, a reduction in the veness of a tumor, reduction in the growth rate of the cancer, or prevention of metastasis. Treatment of cancer may also refer to prolonging survival of a subject with cancer.
An (x-amanitin ate may be administered to treat a cancer. Cancers for which the present treatment methods are useful include any malignant cell type, such as those found in a solid tumor or a hematological tumor. Exemplary solid tumors can e, but are not limited to, a tumor of an organ selected from the group consisting of pancreas, colon, cecum, stomach, brain, head, neck, ovary, kidney, larynx, sarcoma, lung, bladder, melanoma, prostate, and breast. Exemplary logical tumors include tumors of the bone marrow, T or B cell malignancies, leukemias, lymphomas, blastomas, myelomas, and the like. Further examples of cancers that may be treated using the methods provided herein include, but are not d to, lung cancer (including small-cell lung cancer, non-small cell lung cancer, adenocarcinoma of the lung, and squamous carcinoma of the lung), cancer of the neum, gastric or stomach cancer (including intestinal cancer and gastrointestinal stromal cancer), atic cancer, cervical cancer, ovarian cancer, liver cancer, bladder cancer, breast cancer, colon cancer, colorectal cancer, endometrial or uterine carcinoma, salivary gland carcinoma, kidney or renal cancer, prostate cancer, vulval cancer, thyroid cancer, various types of head and neck cancer, and melanoma. id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52" id="p-52"
[0052] The cancer may specifically be of the following histological type, though it is not limited to these: neoplasm, malignant; carcinoma; carcinoma, undifferentiated; giant and spindle cell carcinoma; small cell carcinoma; papillary carcinoma; squamous cell carcinoma; lymphoepithelial oma; basal cell carcinoma; pilomatrix carcinoma; transitional cell carcinoma; papillary transitional cell carcinoma; adenocarcinoma; noma, malignant; cholangiocarcinoma; hepatocellular carcinoma; combined cellular carcinoma and cholangiocarcinoma; trabecular adenocarcinoma; adenoid cystic carcinoma; adenocarcinoma in adenomatous polyp; adenocarcinoma, familial polyposis coli; solid carcinoma; carcinoid tumor, malignant; iolo-alveolar adenocarcinoma; ary adenocarcinoma; chromophobe carcinoma; acidophil carcinoma; oxyphilic adenocarcinoma; basophil carcinoma; clear cell adenocarcinoma; granular cell carcinoma; follicular adenocarcinoma; papillary and follicular arcinoma; nonencapsulating sclerosing carcinoma; adrenal cortical oma; endometroid carcinoma; skin age carcinoma; apocrine adenocarcinoma; sebaceous adenocarcinoma; ceruminous adenocarcinoma; mucoepidermoid carcinoma; cystadenocarcinoma; papillary cystadenocarcinoma; papillary serous cystadenocarcinoma; mucinous cystadenocarcinoma; mucinous adenocarcinoma; signet ring cell oma; infiltrating duct carcinoma; medullary carcinoma; lobular carcinoma; atory carcinoma; paget's disease, mammary; acinar cell carcinoma; adenosquamous carcinoma; arcinoma w/squamous metaplasia; thymoma, malignant; ovarian stromal tumor, malignant; thecoma, ant; granulosa cell tumor, malignant; androblastoma, malignant; sertoli cell carcinoma; leydig cell tumor, malignant; lipid cell tumor, malignant; paraganglioma, malignant; extra-mammary paraganglioma, malignant; pheochromocytoma; glomangiosarcoma; malignant melanoma; amelanotic melanoma; superficial spreading melanoma; lentigo malignant melanoma; acral lentiginous melanomas; nodular melanomas; ant ma in giant pigmented nevus; epithelioid cell melanoma; blue nevus, malignant; sarcoma; fibrosarcoma; fibrous histiocytoma, malignant; myxosarcoma; liposarcoma; leiomyosarcoma; rhabdomyosarcoma; embryonal rhabdomyosarcoma; alveolar rhabdomyosarcoma; stromal sarcoma; mixed tumor, malignant; mullerian mixed tumor; blastoma; hepatoblastoma; carcinosarcoma; mesenchymoma, malignant; brenner tumor, malignant; phyllodes tumor, malignant; synovial sarcoma; mesothelioma, malignant; dysgerminoma; embryonal carcinoma; teratoma, malignant; struma ovarii, malignant; choriocarcinoma; mesonephroma, ant; hemangiosarcoma; hemangioendothelioma, malignant; 's sarcoma; hemangiopericytoma, malignant; lymphangiosarcoma; osteosarcoma; juxtacortical osteosarcoma; osarcoma; chondroblastoma, malignant; mesenchymal osarcoma; giant cell tumor of bone; ewing's sarcoma; odontogenic tumor, malignant; ameloblastic odontosarcoma; ameloblastoma, ant; ameloblastic fibrosarcoma; pinealoma, malignant; ma; glioma, malignant; ependymoma; astrocytoma; protoplasmic astrocytoma; fibrillary astrocytoma; astroblastoma; glioblastoma; oligodendroglioma; oligodendroblastoma; primitive neuroectodermal; cerebellar sarcoma; ganglioneuroblastoma; lastoma; retinoblastoma; olfactory neurogenic tumor; meningioma, ant; neurofibrosarcoma; neurilemmoma, malignant; granular cell tumor, malignant; malignant lymphoma; hodgkin's e; hodgkin's; paragranuloma; malignant lymphoma, small lymphocytic; malignant lymphoma, large cell, diffuse; malignant lymphoma, follicular; s des; other specified non-hodgkin's lymphomas; B-cell lymphoma; low grade/follicular non-Hodgkin's lymphoma (NHL); small lymphocytic (SL) NHL; intermediate grade/follicular NHL; intermediate grade diffuse NHL; high grade immunoblastic NHL; high grade lymphoblastic NHL; high grade small non-cleaved cell NHL; bulky disease NHL; mantle cell lymphoma; AIDS-related lymphoma; Waldenstrom's macroglobulinemia; malignant histiocytosis; le myeloma; mast cell sarcoma; immunoproliferative small intestinal disease; leukemia; lymphoid leukemia; plasma cell leukemia; erythroleukemia; lymphosarcoma cell leukemia; d leukemia; basophilic leukemia; eosinophilic leukemia; monocytic leukemia; mast cell leukemia; megakaryoblastic ia; myeloid sarcoma; hairy cell ia; chronic lymphocytic leukemia (CLL); acute lymphoblastic leukemia (ALL); acute myeloid leukemia (AML); and chronic myeloblastic leukemia.
III. Combination Treatments id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53" id="p-53"
[0053] In certain embodiments, the compositions and methods of the present invention e cell-targeting moieties conjugated to (x-amanitin, in ation with a second or additional therapy. Such therapy can be applied in the treatment of any disease that is associated with hemizygous loss of TP53 and/or POLR2A. For e, the disease may be a cancer. id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54" id="p-54"
[0054] The methods and compositions ing combination therapies enhance the therapeutic or protective effect, and/or increase the eutic effect of another anti-cancer or anti-hyperproliferative therapy. Therapeutic and lactic s and compositions can be provided in a combined amount effective to achieve the desired effect, such as the killing of a cancer cell and/or the inhibition of cellular hyperproliferation. This process may involve contacting the cells with both a cell-targeting moiety conjugated to (x-amanitin and a second therapy. A tissue, tumor, or cell can be contacted with one or more compositions or pharmacological formulation(s) ing one or more of the agents (i.e., an anti-cancer agent), or by contacting the tissue, tumor, and/or cell with two or more distinct compositions or formulations, wherein one composition provides 1) a cell-targeting moiety conjugated to (x-amanitin; 2) an anti-cancer agent, or 3) both a cell-targeting moiety conjugated to 0t- amanitin and an anti-cancer agent. Also, it is contemplated that such a combination therapy can be used in conjunction with a chemotherapy, radiotherapy, surgical y, or immunotherapy.
The terms "contacted" and "exposed," when d to a cell, are used herein to be the process by which a therapeutic antibody and a chemotherapeutic or radiotherapeutic agent are delivered to a target cell or are placed in direct juxtaposition with the target cell. To achieve cell killing, for example, both agents are delivered to a cell in a combined amount effective to kill the cell or prevent it from dividing.
A cell-targeting moiety ated to (x-amanitin may be administered before, during, after or in various combinations relative to an anti-cancer treatment. The administrations may be in intervals ranging from concurrently to minutes to days to weeks.
In embodiments where the tor of gene expression is provided to a patient separately from an anti-cancer agent, one would lly ensure that a significant period of time did not expire between the time of each delivery, such that the two compounds would still be able to exert an advantageously combined effect on the patient. In such instances, it is contemplated that one may provide a patient with the cell-targeting moiety conjugated to 0t- amanitin and the anti-cancer therapy within about 12 to 24 or 72 h of each other and, more particularly, within about 6-12 h of each other. In some situations it may be desirable to extend the time period for treatment significantly where several days (2, 3, 4, 5, 6 or 7) to several weeks (1, 2, 3, 4, 5, 6, 7 or 8) lapse between respective administrations. id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57" id="p-57"
[0057] In certain embodiments, a course of treatment will last 1-90 days, or more (this such range includes intervening days). It is contemplated that one agent may be given on any day of day l to day 90 (this such range includes intervening days) or any ation thereof, and another agent is given on any day of day l to day 90 (this such range includes ening days) or any combination thereof. Within a single day (24-hour period), the patient may be given one or multiple administrations of the agent(s). Moreover, after a course of treatment, it is contemplated that there is a period of time at which no anti-cancer ent is administered. This time period may last 1-7 days, and/or 1-5 weeks, and/or 1-12 months or more (this such range includes intervening days), depending on the condition of the patient, such as their sis, strength, health, etc. It is expected that the treatment cycles would be repeated as necessary.
Various combinations may be employed. For the example below a cell- targeting moiety conjugated to nitin therapy is "A" and an anti-cancer therapy is "B": A/B/AB/A/BB/B/AA/A/BA/B/BB/A/AA/B/B/BB/A/B/B B/B/B/A B/B/A/B A/A/B/B A/B/A/BA/B/B/AB/B/A/A B/A/B/A B/A/A/B A/A/A/BB/A/A/AA/B/A/AA/A/B/A Administration of any compound or therapy of the present invention to a patient will follow general protocols for the administration of such compounds, taking into account the ty, if any, of the agents. Therefore, in some ments there is a step of monitoring toxicity that is attributable to combination therapy.
In specific aspects, it is contemplated that a standard y will e chemotherapy, radiotherapy, immunotherapy, al therapy or gene therapy and may be employed in combination with the inhibitory antibody, anti-cancer therapy, or both the inhibitory antibody and the anti-cancer therapy, as described herein.
A. Chemotherapy id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61" id="p-61"
[0061] A wide variety of chemotherapeutic agents may be used in accordance with the present invention. The term "chemotherapy" refers to the use of drugs to treat cancer. A "chemotherapeutic agent" is used to connote a compound or composition that is administered in the treatment of cancer. These agents or drugs are categorized by their mode of activity within a cell, for example, whether and at what stage they affect the cell cycle. Alternatively, an agent may be characterized based on its y to directly cross-link DNA, to intercalate into DNA, or to induce chromosomal and mitotic aberrations by ing nucleic acid synthesis. Most chemotherapeutic agents fall into the following categories: ting agents, antimetabolites, antitumor antibiotics, mitotic inhibitors, and nitrosoureas.
Examples of chemotherapeutic agents include alkylating agents such as thiotepa and cyclosphosphamide; alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including altretamine, triethylenemelamine, trietylenephosphoramide, triethiylenethiophosphoramide and trimethylolomelamine; enins (especially bullatacin and bullatacinone); a thecin (including the synthetic analogue can); bryostatin; callystatin; 5 (including its adozelesin, carzelesin and bizelesin synthetic analogues); cryptophycins (particularly phycin l and cryptophycin 8); dolastatin; duocarmycin (including the synthetic analogues, KW-2189 and CBl-TMl); eleutherobin; pancratistatin; a sarcodictyin; spongistatin; nitrogen mustards such as chlorambucil, aphazine, cholophosphamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, novembichin, phenesterine, prednimustine, trofosfamide, uracil mustard; nitrosureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, and ustine; antibiotics such as the enediyne antibiotics (e. g., calicheamicin, especially calicheamicin gammalI and calicheamicin omegaIl; dynemicin, including dynemicin A; bisphosphonates, such as clodronate; an esperamicin; as well as neocarzinostatin chromophore and related chromoprotein enediyne antiobiotic chromophores, aclacinomysins, actinomycin, authramycin, azaserine, cins, cactinomycin, cin, carminomycin, carzinophilin, chromomycinis, dactinomycin, ubicin, detorubicin, 6-diazooxo-L-norleucine, doxorubicin (including morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolino- doxorubicin and deoxydoxorubicin), epirubicin, esorubicin, idarubicin, marcellomycin, mitomycins such as mitomycin C, mycophenolic acid, rnycin, ycins, peplomycin, potfiromycin, cin, quelamycin, rodorubicin, onigrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; anti-metabolites such as methotrexate and 5- ?uorouracil (5-FU); folic acid analogues such as denopterin, pteropterin, trimetrexate; purine analogs such as ?udarabine, 6-mercaptopurine, thiamiprine, thioguanine; pyrimidine analogs such as ancitabine, azacitidine, 6-azauridine, ur, cytarabine, dideoxyuridine, doxi?uridine, enocitabine, ?oxuridine; androgens such as calusterone, dromostanolone propionate, epitiostanol, mepitiostane, testolactone; anti-adrenals such as mitotane, trilostane; folic acid replenisher such as frolinic acid; aceglatone; osphamide glycoside; evulinic acid; eniluracil; amsacrine; bucil; bisantrene; xate; defofamine; lcine; diaziquone; elformithine; elliptinium acetate; an epothilone; etoglucid; m nitrate; hydroxyurea; lentinan; lonidainine; maytansinoids such as maytansine and ansamitocins; mitoguazone; mitoxantrone; mopidanmol; rine; pentostatin; phenamet; bicin; losoxantrone; podophyllinic acid; 2-ethylhydrazide; procarbazine; PSKpolysaccharide complex; ne; rhizoxin; sizofiran; spirogermanium; tenuazonic acid; triaziquone; 2,2,2"-trichlorotriethylamine; trichothecenes (especially T-2 toxin, verracurin A, roridin A and anguidine); urethan; vindesine; dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine; arabinoside ("Ara-C"); cyclophosphamide; taxoids, e. g., paclitaxel and docetaxel gemcitabine; 6-thioguanine; mercaptopurine; platinum nation complexes such as cisplatin, oxaliplatin and carboplatin; vinblastine; platinum; ide (VP-l6); ifosfamide; mitoxantrone; vincristine; vinorelbine; novantrone; teniposide; edatrexate; daunomycin; aminopterin; xeloda; ibandronate; irinotecan (e. g., CPT- ll); topoisomerase inhibitor RFS 2000; di?uorometlhylornithine (DMFO); retinoids such as retinoic acid; capecitabine; carboplatin, bazine,plicomycin, gemcitabien, navelbine, famesyl-protein tansferase inhibitors, transplatinum, and pharmaceutically acceptable salts, acids or derivatives of any of the above.
Also included in this definition are anti-hormonal agents that act to regulate or inhibit hormone action on tumors such as anti-estrogens and selective estrogen receptor modulators (SERMs), ing, for example, tamoxifen, raloxifene, droloxifene, 4- hydroxytamoxifen, trioxifene, ene, LY117018, onapristone, and fene; aromatase inhibitors that inhibit the enzyme aromatase, which regulates estrogen tion in the adrenal glands, such as, for example, 4(5)-imidazoles, aminoglutethimide, megestrol acetate, exemestane, formestanie, fadrozole, vorozole, letrozole, and anastrozole; and anti-androgens such as ?utamide, nilutamide, bicalutamide, leuprolide, and goserelin; as well as troxacitabine (a 1,3-dioxolane side cytosine ); antisense oligonucleotides, particularly those which inhibit expression of genes in signaling ys implicated in nt cell proliferation, such as, for example, PKC—alpha, Ralf and H-Ras; ribozymes such as a VEGF expression inhibitor and a HER2 expression inhibitor; vaccines such as gene y vaccines and pharmaceutically acceptable salts, acids or derivatives of any of the above.
B. Radiotherapy Other factors that cause DNA damage and have been used extensively include what are commonly known as y-rays, X-rays, and/or the directed delivery of radioisotopes to tumor cells. Other forms of DNA damaging factors are also contemplated, such as microwaves, proton beam irradiation (U.S. s 5,760,395 and 4,870,287) and UV- irradiation. It is most likely that all of these s affect a broad range of damage on DNA, on the precursors of DNA, on the replication and repair of DNA, and on the assembly and maintenance of chromosomes. Dosage ranges for X-rays range from daily doses of 50 to 200 roentgens for prolonged periods of time (3 to 4 wk), to single doses of 2000 to 6000 roentgens. Dosage ranges for radioisotopes vary , and depend on the half-life of the isotope, the strength and type of radiation d, and the uptake by the neoplastic cells.
The terms "contacted" and "exposed," when applied to a cell, are used herein to describe the process by which a therapeutic construct and a chemotherapeutic or radiotherapeutic agent are delivered to a target cell or are placed in direct juxtaposition with the target cell. To achieve cell killing, for example, both agents are delivered to a cell in a combined amount effective to kill the cell or prevent it from dividing.
C. Immunotherapy In the context of cancer ent, immunotherapeutics, generally, rely on the use of immune effector cells and molecules to target and destroy cancer cells. Trastuzumab ptinTM) is such an e. The immune effector may be, for example, an antibody specific for some marker on the surface of a tumor cell. The antibody alone may serve as an effector of therapy or it may recruit other cells to actually affect cell g. The antibody also may be conjugated to a drug or toxin (chemotherapeutic, radionuclide, ricin A chain, cholera toxin, pertussis toxin, etc.) and serve merely as a targeting agent. Alternatively, the effector may be a lymphocyte ng a surface molecule that interacts, either directly or ctly, with a tumor cell . Various effector cells include cytotoxic T cells and NK cells. The combination of therapeutic modalities, i.e., direct cytotoxic activity and inhibition or reduction of ErbB2 would provide therapeutic benefit in the treatment of ErbB2 overexpressing s.
Another immunotherapy could also be used as part of a combined therapy with gene silencing therapy discussed above. In one aspect of immunotherapy, the tumor cell must bear some marker that is amenable to targeting, i.e., is not t on the majority of other cells. Many tumor markers exist and any of these may be suitable for targeting in the context of the present invention. Common tumor markers include carcinoembryonic antigen, prostate specific antigen, urinary tumor associated antigen, fetal antigen, tyrosinase (p97), gp68, TAG-72, HMFG, Sialyl Lewis Antigen, MucA, MucB, PLAP, estrogen receptor, n receptor, erb B and p155. An alternative aspect of immunotherapy is to combine anticancer effects with immune stimulatory effects. Immune stimulating molecules also exist including cytokines, such as IL-2, IL-4, IL-12, GM-CSF, and gamma-IFN, chemokines, such as MIP—l, MCP-l, and IL-8, and growth factors, such as FLT3 ligand. Combining immune stimulating molecules, either as proteins or using gene delivery in combination with a tumor suppressor has been shown to enhance umor effects (Ju et al., 2000). Moreover, antibodies against any of these compounds can be used to target the anti-cancer agents discussed herein.
Examples of immunotherapies currently under investigation or in use are immune adjuvants e. g., cterium bovis, Plasmodium falciparum, dinitrochlorobenzene and aromatic compounds (U.S. Patents 5,801,005 and 169; Hui and oto, 1998; Christodoulides et al., 1998), cytokine therapy, e. g., interferons 0c, [3 and 7; IL-1, GM-CSF and TNF (Bukowski et al., 1998; Davidson et al., 1998; Hellstrand et al., 1998) gene therapy, e. g., TNF, IL-1, IL-2, p53 (Qin et al., 1998; Austin-Ward and Villaseca, 1998; U.S. Patents ,830,880 and 5,846,945) and monoclonal antibodies, e. g., anti-ganglioside GM2, anti-HER- 2, anti-p185 (Hanibuchi et al., 1998; U.S. Patent 311). It is contemplated that one or more anti-cancer therapies may be employed with the gene silencing therapies bed herein.
In active immunotherapy, an antigenic peptide, polypeptide or protein, or an autologous or allogenic tumor cell composition or "vaccine" is administered, generally with a distinct bacterial nt. In adoptive immunotherapy, the patient's ating lymphocytes, or tumor infiltrated lymphocytes, are isolated in vitro, activated by lymphokines, such as IL- 2, or transduced with genes for tumor necrosis, and re-administered.
D. Surgery imately 60% of persons with cancer will o surgery of some type, which includes preventative, diagnostic or staging, curative, and palliative surgery.
Curative surgery is a cancer treatment that may be used in conjunction with other therapies, such as the treatment of the present invention, herapy, radiotherapy, hormonal therapy, gene y, immunotherapy and/or alternative therapies.
Curative surgery includes ion in which all or part of cancerous tissue is physically removed, excised, and/or destroyed. Tumor resection refers to physical l of at least part of a tumor. In addition to tumor resection, treatment by surgery includes laser surgery, cryosurgery, electrosurgery, and microscopically controlled surgery (Mohs’ surgery). It is further contemplated that certain aspects of the present invention may be used in conjunction with removal of icial s, cers, or incidental amounts of normal tissue. id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72" id="p-72"
[0072] Upon excision of part or all of cancerous cells, tissue, or tumor, a cavity may be formed in the body. Treatment may be accomplished by perfusion, direct injection or local application of the area with an additional anti-cancer therapy. Such treatment may be repeated, for example, every 1, 2, 3, 4, 5, 6, or 7 days, or every 1, 2, 3, 4, and 5 weeks or every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 months. These treatments may be of varying dosages as well.
E. Other Agents It is contemplated that other agents may be used in combination with certain aspects of the present invention to improve the therapeutic efficacy of treatment. These additional agents include immunomodulatory agents, agents that affect the upregulation of cell surface receptors and GAP ons, cytostatic and differentiation , inhibitors of cell adhesion, agents that se the sensitivity of the hyperproliferative cells to apoptotic inducers, or other biological agents. modulatory agents include tumor is factor; interferon alpha, beta, and gamma; IL-2 and other cytokines; F42K and other cytokine analogs; or MIP-l, MIP-lbeta, MCP-l, RANTES, and other chemokines. It is further contemplated that the upregulation of cell surface receptors or their ligands such as Fas / Fas ligand, DR4 or DR5 / TRAIL (Apo-2 ligand) would potentiate the apoptotic inducing abilities of the present invention by establishment of an autocrine or paracrine effect on hyperproliferative cells. Increase of ellular signaling by elevating the number of GAP ons would increase the anti-hyperproliferative effects on the neighboring hyperproliferative cell population. In other embodiments, cytostatic or differentiation agents can be used in combination with certain aspects of the present invention to improve the anti- hyperproliferative efficacy of the treatments. Inhibitors of cell adhesion are contemplated to improve the efficacy of the present invention. Examples of cell adhesion inhibitors are focal adhesion kinase (FAKs) inhibitors and Lovastatin. It is further plated that other agents that increase the sensitivity of a hyperproliferative cell to apoptosis, such as the antibody c225, could be used in combination with certain aspects of the present invention to improve the treatment efficacy.
Hormonal therapy may also be used in conjunction with n aspects of the t invention or in combination with any other cancer therapy previously described. The use of hormones may be employed in the treatment of certain cancers such as breast, prostate, ovarian, or cervical cancer to lower the level or block the effects of certain hormones, such as terone or en. This ent is often used in combination with at least one other cancer therapy as a treatment option or to reduce the risk of metastases.
IV. Examples id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75" id="p-75"
[0075] The following examples are included to demonstrate preferred embodiments of the ion. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to on well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without ing from the spirit and scope of the invention.
Materials and Methods Cell culture, antibodies, and western blot analysis. HCT116, SW480, SW837, HT29, DLD1, and HT29 cell lines were ed from the American Type Culture Collection and cultured under standard conditions specified by the manufacturer. SNU283 and SNU1197 cell lines were obtained from the Korean Cell Line Bank and cultured in RMPI 1640 medium supplemented with 10% FBS and 2 mM L-Glutamine. Isolation, e and maintenance of xenografted human y CRC (XhCRC) cells were performed as previously described (Lu et al., 2013). Brie?y, the patient-derived xenografts were harvested under e conditions and mechanically dissociated, ed by 30 min of incubation in collagenase II at 370C. The specimen was filtered through a sterile 100-um er. Red blood cells were eliminated with a hypo-osmotic red blood cell lysis buffer (eBioscience).
Mouse cells in xenografted human CRC specimens were removed by negative selection using a mouse MHC class I molecule H-2K antibody followed by use of a ic bead purification kit (Miltenyi). Fibroblasts were removed by negative selection using a MACS magnetic bead separation kit (Miltenyi). The freshly ed XhCRC cells were maintained on Collagen-1 coated culture plates (BD Biosciences) and cultured in MEM supplemented with 10% FBS, vitamins (1x), nonessential amino acids (1x), Pen-Strep (1x), sodium pyruvate (1x), and L-glutamine (1x). All medium supplements were purchased from Sigma.
Anti-POLR2A antibodies were purchased from Santa Cruz (#sc-47701) and Abcam (#ab140509). Anti-Ki67 antibody (#D3B5) and anti-cleaved Caspase-3 (Asp175, #5A1E) antibody were obtained from Cell Signalling. Anti-p53 (#sc-126), anti-B-actin (#sc- 1616), HRP-anti-goat IgG 020), HRP-anti-rabbit IgG (#sc-2054), and HRP-anti-mouse IgG 055) antibodies were purchased from Santa Cruz. Cell lysate preparation, SDS- PAGE, and n blotting were performed as previously described (Liu et al., 2012). id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78" id="p-78"
[0078] RNA isolation and quantitative PCR. Total RNA was isolated using TRIzol reagent (Life Technologies) and then reverse-transcribed using iScript cDNA Synthesis Kit (Bio-Rad). The resulting cDNA was used for qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad) with gene-specific primers and the s were normalized to B-actin as a control. In RT-PCR assays, the primer sequences for TP53 are: 5’- GAGGTTGGCTCTGACTGTACC—3’ (SEQ ID NO: 1) and 5 ’ - TCCGTCCCAGTAGATTACCAC-3’ (SEQ ID NO: 2), and for POLR2A are: 5’- TTGTATCCGTACCCACAGCA-3’ (SEQ ID NO: 3) and 5 ’ - CATGATCAGCTCCCCATTCT-3’ (SEQ ID NO: 4). shRNA-mediated knockdown of POLR2A. -specific shRNA clones were obtained from the MD Anderson shRNA and ORFeome Core Facility nally from Open Biosystems). Twelve shRNAs targeting POLR2A were screened, of which two shRNAs knocked down protein levels by at least 50% in all four ctal cancer cell lines tested. The clone identification s and shRNA sequences are V3LHS_645674 (5’- TTAGCTTTGTTCTTCCCGA-3’ [SEQ ID NO: 5]) and V3LHS_64029 (5’- TGTTGTCCATCTCCTCCCC—3’ [SEQ ID NO: 6]). The hairpin sequences in the GIPZ vector were cloned into the TRIPZ vector (Dharmacon) using a protocol provided by the manufacturer. The TRIPZ vector is a Dox-inducible system with a red ?uorescent protein reporter.
Generation of cells stably expressing Box-inducible shRNAs. Recombinant lentiviral particles were produced from 293T cells. Brie?y, 72 ug of shRNA-encoding vector DNA, 54 ug of Delta 8.9 vector DNA and 18 ug of VSVG-encoding vector DNA were ected into 293T cells (plated in the 245-mm2 dish) using X-tremeGENE (Roche).
Supernatant containing virus particles was collected and filtered 72 h after transfection, concentrated by ultracentrifugation at 90,000g, and resuspended in cell growth medium. To generate stable Dox-inducible cells, HCT116 and SNU283 cells were infected with shRNA- expressing viral particles at the multiplicity of ion (MOI) of 1. Viral solutions were added to cell culture medium containing 4 ug/mL polybrene. Cells were selected 48 h after infection by 2 ug/ mL puromycin. Single colonies were ed and propagated, and colonies bearing a single copy of lentiviral DNA insert were identified and analyzed for own efficiency. id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81" id="p-81"
[0081] Competition assay using POLR2A shRNA. A single lentiviral copy expressing either l or shRNA-2 was sufficient to suppress POLR2A expression levels in all the four colorectal cell lines tested (). Cancer cells were ed with control or POLR2A shRNA-expressing lentiviruses (pGIPZ backbone expressing GFP) at the MOI of 2.
Two days after infection, GFP-positive cells were sorted using a BD FACSJazzTM cell sorter (BD Biosciences) at the MD Anderson Flow Cytometry and Cellular Imaging Core Facility.
Next, GFP—positive cells were mixed with fected and GFP-negative cells at the ratio of 1:1 and cultured for six passages. The numbers of GFP-positive and total cells at each passage were ed and quantified by ?ow cytometry and the percentages of GFP-positive cells were calculated.
Generation of xpressing vectors and Surveyor assay. Bicistronic expression vector expressing Cas9 and ngNA was digested with BbsI and treated with ne phosphatase, and the ized vector was gel purified for cloning ngNA-encoding DNA (Cong et al., 2013). The pair of oligo DNA for each ngNA targeting TP53 or POLR2A was annealed, phosphorylated, and ligated to the linearized vector. The ces of oligo DNA are listed in Table 1. Surveyor assay was performed to test the genome editing efficacy as usly described (Ran et al., 2013; Guschin et al., 2010). , cells were seeded into six-well plates at a density of 2 x 105 cells per well. One day after initial seeding, cells were transfected with 2 ug of Cas9/ngNA-expressing vector DNA and harvested 48 h after transfection. Genomic DNA was isolated and a 1kb DNA fragment containing the ngNA- targeting site was amplified by high-fidelity PCR and digested by T7 endonuclease I.
Genomic DNA isolated from HCT116 cells transfected with control vector DNA was used as control. To allow complementary but mismatched strands to anneal, PCR products were incubated at 95 0C for 5 min, 95 0C to 85 0C at the rate of -2 0C s‘1 and 85 0C to 25 0C at the rate of -0.1 0C s‘l. T7 endonuclease I was added and samples were incubated at 37 0C for 60 min to digest the annealed PCR products at the mismatch sites. T7 clease sted PCR products were analyzed by agarose gel electrophoresis. Oligonucleotide sequences used for PCR amplification are listed in Table 1. The PCR products from positive clones were ligated to pGEM-T Easy Vector (Promega) and further confirmed by DNA sequencing.
Table 1. Sequence of oligonucleotides for ngNAs and Surveyor assays.
Genomic Sequence target POLR2A cacchGGCCTCCCTCAGTCGTCTC Pol-sle POLR2A aaacGAGACGACTGAGGGAGGCCGC Pol-sp2F POLR2A caccGGCCGCTGCCAAACATGTGC ngNA Pol-sp2R POLR2A aaacGCACATGTTTGGCAGCGGCC ngNA Pol- POLR2A AAAATCTCCATCTGGACACGAAAGG Surveyor Name Genomic Sequence SEQ ID Assay target NO: Pol- POLR2A AGCGCAAAACTTTCATTGTCTTCAC 12 Surveyor gDNAR p53- TP53 ATTGTTCAATATCGTCCG 13 ngNA spF1F p53- TP53 aaacCGGACGATATTGAACAATGGC 14 ngNA spRlR p53- TP53 GCAGCTACGGTTTCCGTC 15 ngNA spF2F p53- TP53 aaacGACGGAAACCGTAGCTGCCC 16 ngNA spR2R p53- TP53 GAGGAGCCGCAGTCAGATCCTA 17 Surveyor gDNAF p5 3- TP53 GATACGGCCAGGCATTGAAGTC 18 Surveyor gDNAR Cell proliferation and survival assay. Equal numbers of cells were plated in 12-well plates in triplicate. Cells were fixed with 10% methanol and stained with 0.1% crystal violet lved in 10% methanol) at indicated times. After staining, wells were washed three times with PBS and destained with acetic acid. The absorbance of the crystal violet solution was measured at 590 nm. For cell survival assay, cells were seeded at a concentration of 1,000 cells per well in 96-well plates and treated with indicated concentrations of (x-Amanitin or actinomycin D 24 hours later. Cell viability was quantified using WST-1 reagent (Roche) ing to the manufacturer's instructions. All ments were med in triplicate. sis and cell cycle analysis. The HCT116 and SNU283 cell lines were treated with or without (x-Amanitin for 2 d or doxycycline for 4 d at indicated concentrations and d with annexin V-PE and 7-AAD (Biovision). Apoptosis was analyzed by ?ow cytometry using a Guava EasyCyte Flow Cytometer (Millipore) according to the manufacturer’s protocol. Both optotic (annexin V-positive and 7-AAD-negative) and apoptotic (annexin V-positive and 7-AAD-positive) cells were included in the analyzes. For cell cycle analysis, cells were fixed in 75% ethanol at -20 oC overnight. The cells were washed with cold PBS, treated with 100 ug of RNase A (Qiagen), and d with 50 ug of propidium iodide (Roche). Cell cycle profiles were analyzed by ?ow cytometry using the Guava EasyCyte Flow Cytometer (Millipore).
Fluorescence in sita hybridization (FISH). FISH analysis was performed using Fluorescein-labelled POLR2A (red) and control centromere (Chr 17, green) probes from Empire Genomics. Hybridization and detection were performed according to the cturer’s protocols. The slides were rstained with DAPI, and the images were captured using a Nikon E800 microscope equipped with a -charge coupled device (CCD) camera. To determine hemizygous loss of the POLR2A gene, 100 individual nuclei were analyzed for each case. The interphase nuclei were captured and processed using the tative Image Processing System (Applied Imaging).
Patient samples. Matched normal and colorectal tumor tissue samples from patients were obtained from the MD Anderson Cancer Center (MDACC) through appropriate informed consents after approval by the institutional review board (IRB# PA11-0767). To determine the expression levels of POLR2A protein, tissue samples (20—40 mg) were placed in tubes containing ceramic beads and were homogenized using a Precellys 24 homogenizer device (Bertin Technologies). The lysates were spun-down twice (15 min, 16,000g) and the supernatant was collected.
Genomic DNA isolation and copy number validation. Total c DNA was extracted from human tissue specimens and cell lines using DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer’s purification instructions. All the DNA samples were stored at -200C. The copy number variations for POLR2A were determined using TaqMan probes (Hs02023849_cn and Hs01252684_cn) and standard TaqMan PCR kit on an Applied Biosystems 7900HT Sequence Detection System. And the reference gene TERT was simultaneously quantified in the same tube for each DNA .
Conjugation of a—Amanitin to pCAM antibody (HEA125). Antibody- drug conjugate A125 was constructed by coupling of oc-Amanitin to lysine residues of HEA125 antibody by a stable linker structure. HEA125 binds to EpCAM-expressing cells with high ty (KD of approximately 2.2 x 10‘9 M) and high specificity. HEA125 was purified by affinity chromatography using a protein A-Sepharose CL-4B column (GE Healthcare). (x-Amanitin was attached to immunoglobulin molecules by a plasma stable urea e intended to release free (x-Amanitin inside the tumor cell after lysosomal degradation of the antibody . The drug-antibody ratio (DAR) of the (x-AmanitinzlgG molecule was 4:1. Biochemical characteristics of A125 were evaluated by high performance liquid chromatography (HPLC) using a PlatinBlue HPLC system (Knauer). In addition, HEA125 and A125 were analyzed by reducing GE and Coomassie staining according to common procedures. Verification of drug-loading was done by anti-amanitin immunoblotting analysis of 30 ng HEA125 and ama-HEA125 using rd techniques.
Liposomal nanoparticle preparation. siRNAs for in vivo delivery were encapsulated into DOPC (1,2-dioleoyl-sn-glycerophosphatidylcholine). DOPC and siRNA were mixed in the presence of excess tertiary l at a ratio of 1:10 (w/w) DOPC.
Tween 20 was added to the mixture in a ratio of 1:19 Tween 20:siRNA/DOPC. The mixture was vortexed, frozen in an acetone/dry ice bath, and lyophilized. Prior to in vivo administration, this preparation was hydrated with PBS at room temperature at a concentration of 150-1000 ug siRNA/kg per injection (each mouse received 200 uL of DOPC:siRNA:PBS on by the intraperitoneal route).
Xenograft tumor studies. Four- to six-week-old female NOD/SCID mice were purchased from Jackson Laboratories and housed under pathogen-free conditions. All studies were approved and supervised by the Institutional Animal Care and Use Committee of MD Anderson Cancer Center. When used in a power calculation, the present sample size predetermination experiments indicated that 5 mice per group can identify the expected effect of POLR2A on tumor size and weight (p < 0.05) with 90% power. Animals were randomly divided to ent groups. Dox-inducible HCT116 (1 x 106) and SNU283 (2 x 106) cells in 50 ul growth medium (mixed with Matrigel at 1:1) were injected subcutaneously into the ?ank using a 100-ul Hamilton microliter syringe. Tumor size was measured every five days using a caliper, and tumor volume was ated using the rd formula: 0.5 x L x W2, where L is the longest diameter and W is the shortest diameter. For orthotopic mouse model, the SCID mice were anaesthetized and the skin was incised to expose cecum. Dox-inducible HCT116 cells (1 x 106) expressing luciferase were injected into the cecal wall using a 100-ul on microliter syringe, and then the incision was closed using wound clips. Tumors were monitored by the IVIS system after luciferin injection for 15 min. After initial establishment of tumor (100 mm3 for subcutaneous implants and 2 x 108 photons/second, total ?ux for orthotopic implants), mice were treated with 1 ug ml'1 doxycycline in ng water for 3 to 4 weeks. The doxycycline water was changed every other day.
For xenograft tumor studies using DOPC-encapsulated , isogenic pairs of HCT116 (1 x 106) cells were transplanted into the cecal wall using a 100-ul Hamilton microliter syringe. Ten days following cell injection, mice were randomly divided and assigned to e either control siRNA-DOPC or POLR2A siRNA-DOPC. The siRNA sequences are as follows: control siRNA (5’-UUCUCCGAACGUGUCACGU-3’ [SEQ ID NO: 19] and 5’-ACGUGACACGUUCGGAGAA-3’ [SEQ ID NO: 20]); POL2 siRNA-l (5’- CCAACAUGCUGACAGAUAU-3’ [SEQ ID NO: 21] and 5 ’ - AUAUCUGUCAGCAUGUUGG-3’ [SEQ ID NO: 22]) ; POL2 siRNA-2 (5 ’ - CCAAGAAGCGGCUCACACA-3’ [SEQ ID NO: 23] and 5 ’ - UGUGUGAGCCGCUUCUUGG-3’ [SEQ ID NO: 24]). A dose of 150 to 1000 ug siRNA/kg mouse was administrated intraperitoneally at twice weekly intervals. This range of concentrations ensures efficient delivery and own of target genes, as usly described (Pecot et al., 2014). id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92" id="p-92"
[0092] For xenograft tumor studies using 0t-Amanitin-HEA125 antibody-drug conjugate (ADC), isogenic pairs of HCT116 (1 x 106), XhCRC (0.5 x 106), SW480 (1 x 106) or SW837 (2 x 106) cells were lanted into the cecal wall using a 100-ul Hamilton microliter syringe. Mice bearing 10-day-old tumors were randomized to five groups (n = 10 mice/group) and received two intraperitoneal doses (1 week apart) of the ing: 1) l unconjugated HEA125 mAb at a dose of 3.6 mg kg'1 of body weight; 2) Ama- HEA125 at a dose of 90 ug kg'1 in terms of (x-Amanitin (corresponding to 3.6 mg kg'1 of IgG); 3) Ama-HEA125, 30 ug kg'1 in terms of (x-Amanitin (corresponding to 1.2 mg kg'1 of IgG); 4) Ama-HE125, 10 ug kg'1 in terms of (x-Amanitin (corresponding to 0.4 mg kg'1 of IgG); and 5) Ama-HEA125, 3 ug kg'1 in terms of (x-Amanitin (corresponding to 0.12 mg kg'1 of IgG). Tumors were monitored by the IVIS live imaging system twice a week after luciferin injection for 15 min. Body weights were recorded every four days. Blood was obtained by retro-orbital bleeding after anesthesia on day 21 and levels of blood liver enzymes (AST: aspartate amino transferase, ALT: amino alanine transferase and alkaline phosphatase) were determined at the Clinical Pathology, Veterinary Medicine and Surgery Core of MD Anderson Cancer Center.
Mice were euthanized when they met the institutional asia criteria for tumor size and overall health condition. Tumors were removed, photographed and weighed.
The freshly dissected tumor tissues were fixed in 10% buffered formalin overnight, washed with PBS, erred to 70% ethanol, embedded in paraffin, sectioned and d with haematoxylin and eosin.The igators were blinded to the group allocation during the experiment and when assessing the outcome.
Immunohistochemistry and human colon tissue microarray. Colon cancer tissue microarray (BC051110a) was purchased from Biomax, including 110 colon tumor samples and 10 normal colon tissue samples. Samples were deparaffinized and ated.
Antigen was retrieved using 0.01 M sodium-citrate buffer (pH 6.0) at a sub-boiling temperature for 10 min after boiling in a microwave oven. To block endogenous peroxidase activity, the sections were incubated with 3% hydrogen peroxide for 10 min. After 1 h of pre- incubation in 5% normal goat serum to prevent nonspecific staining, the samples were incubated with antibody against POLRZA (#sc-47701, Santa Cruz), Ki67, (#D3B5, Cell Signaling), or cleaved Caspase-3 (#5A1E, Cell ing) at 4 oC overnight. The ns were ted with a biotinylated secondary antibody (4Plus Biotinylated ouse or anti-rabbit IgG, E) and then incubated with avidin—biotin peroxidase x on and developed using a DAB (diaminobenzidine) Substrate Kit (#550880, BD Biosciences) according to the manufacturer's protocol. Counterstaining color was carried out using Harris modified haematoxylin. All immunostained slides were scanned on the Automate Cellular Image System 111 (ACIS III) for fication by digital image analysis.
Bioinformatic analysis. The correlation n gene copy number and the corresponding gene sion was analyzed using data obtained from CCLE (on the world wide web at broadinstitute.org/ccle) and TCGA (on the world wide web at rtal.org/public-portal/) as previously described (Nijhawan et al., 2012). Enrichment of Pearson correlation coefficients was determined by permuting gene names. To determine whether a deleted gene functions as a housekeeping gene, its expression profiles in tumor and normal tissues as well as its general functions from literature were first analyzed. Second, gene conservation across species and lethality of the gene knockout were checked by searching available databases of model organisms (Saccharomyces Genome Database, WormBase, FlyBase, and Mouse Genome Informatics). Third, the proximity of the potential target gene to the TP53 gene was d and its co-deletion with TP53 in human cancers was analyzed. Finally, cancer cell lines with the deletion of TP53 and the target gene were identified to test the present hypothesis.
Statistical analysis. Each ment was repeated three times or more.
Unless otherwise noted, data are presented as mean and s.d. or s.e.m., and t’s t-test (unpaired, two-tailed) was used to compare two groups of independent samples. In an unpaired t-test, equal variance was assumed and no samples were excluded from the analysis.
Statistical methods used for TCGA data analysis are described above. p < 0.05 was considered statistically significant.
Example 1 - Expression of POLR2A, but not TP53, is correlated with gene copy number c deletion of a tumor suppressor gene often encompasses multiple oring genes that may not bute to cancer development, but are essential for cell proliferation and survival (Negrini et al., 2010). This partial loss of such eeping genes has been postulated to render cancer cells highly vulnerable to further inhibition of those genes (Nijhawan et al., 2012). Analysis of TCGA revealed that hemizygous deletion of the TP53 gene occurs frequently in a wide array of human cancers (). POLR2A was identified as a housekeeping gene in the proximity of TP53 (residing ~ 200 kilobases away in human genome) that is essential for cell survival (). Concomitant deletion of POLR2A occurs in virtually all the human colorectal tumors harbouring hemizygous deletion of TP53 ().
Among the twelve subunits in human RNA polymerase II complex, POLR2A s the largest subunit that is indispensable for the rase activity in mRNA synthesis (Shalem et al., 2014). Inhibiting POLR2A with a specific inhibitor, nitin, causes extensive cell death, and furthermore, homozygous deletion of POLR2A is lethal in human cells (Lindell et al., 1970; Shalem et al., 2014). It was found that 104 (53%) out of 195 colorectal cancer (CRC) cases bear partial loss of the 17p13 region, resulting in concomitant deletion of TP53 and POLR2A (). However, no gous deletion of POLR2A was observed, consistent with the notion that POLR2A is essential for cell survival.
Analysis of TCGA and CCLE databases revealed that expression of POLR2A is tightly correlated with its gene copy number (). This positive correlation was also validated in twenty pairs of matched normal and CRC tissue samples and human CRC tissue microarray (FIGS. 1E, 5A, and 5B and Table 2). The copy numbers of POLR2A were ined in a set of colorectal cancer cells () and found that all the three POLR2Aloss (hemizygous loss of POLR2A) cell lines expressed POLR2A proteins at significantly lower levels than POLR2AHC'ltral cell lines (FIGS. 1G and 1H). Unlike POLR2A, p53 levels are ined by a complexity of post-transcriptional and post- translational events (Toledo and Wahl, 2006). Despite a correlation between TP53 copy number and mRNA expression, p53 protein levels are not ated with the gene copy numbers in colorectal tumors and cell lines (FIGS. 6A-6C and 1H).
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Table 2 con’t .Adsemsnzrcsrimmx ?ximoeauwm Nemm mimic {same Ream? mienére {issue Teéemmi mimic- :rrrare Noam? whim. ?remmi c 'iiS?llQ‘ Example 2 - POLRZAloss cells are highly sensitive to POLRZA inhibition To assess the ivity of cells with or without TP53/POLR2A hemizygous loss to POLRZA inhibition, a panel of POLRZAHC'ltral (HCT116, SW480) and POLRZAloss , SNU283) cells were treated with (x-Amanitin. Treatment of (x-Amanitin at high concentrations (2 1 ug ml'l) drastically inhibited cell growth and caused complete cell death in all four cell lines. r, at concentrations from 0 to 1.0 ug ml'l, (x-Amanitin inhibition had significantly higher levels of cell-killing effect on the POLRZAloss cells than on the control POLRZAHC'ltral cells (FIGS. 2A and 2B). The half-maximum inhibitory concentration (ICso) was ~1.0 ug ml"1 for the POLRZAHC'ltral cells, which was 10-fold greater than that of the POLRZAloss cells. By contrast, the POLRZAloss cells did not show any greater sensitivity to the treatment of actinomycin D, a cific transcription inhibitor (). Next, the vulnerability of POLRZAloss cells was evaluated using direct competition assay. Cell proliferation rates were compared between control cells and GFP-positive cells expressing control or POLR2A-specific shRNA. Two independent shRNAs knocked down POLRZA expression by 50%-70% in all the tested cell lines (). After culturing for six passages, the POLRZAHC'ltral cells (HCT116, SW480) stably sing POLRZA shRNAs only had modestly reduced eration, in comparison with that of the corresponding cells expressing control shRNAs (). However, silencing POLR2A in the POLR2Aloss cells (SNU283, SW837) led to markedly reduced proliferation, suggesting that gous loss of POLR2A renders cancer cells more prone to further POLR2A inhibition. HCT116 and SNU283 cell lines stably expressing doxycycline (Dox)-inducible POLR2A shRNAs were generated (). Despite significant knockdown of POLR2A, HCT116 cells continued to proliferate, whereas SNU283 cells exhibited severe G1 cell cycle arrest and apoptosis (FIGS. 2C, 2D, and 7E-7G). Approximately 50% of decrease in POLR2A expression (with the on of -100 ng ml'1 of Dox) remarkably d the proliferation of SNU283 cells, but only had a modest effect on HCT116 cells (). Next, rescue experiments were performed in the POLR2Aloss SNU283 and SW837 cell lines. l re-expression of exogenous POLR2A in both cell lines restored their resistance to (x-Amanitin up to a level comparable to that of the POLRZAHC'ltral HCT116 and SW480 cells (FIGS. 2E, 2F, and 8A-8B).
To e the effects of various genetic backgrounds across cell lines, the CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 system was employed to generate an isogenic HCT116 cell line with gous loss of POLR2A (Wang et al., 2014; Wang et al., 2013). Two single-guide RNAs (ngNAs) were ed to target the second exon of the POLR2A gene (). Both of them efficiently targeted and disrupted the POLR2A gene, as shown in the Surveyor is (). Single colonies of HCT116 cells bearing mono-allelic deletion of POLR2A were ed and verified by DNA sequencing (). Small deletion in the targeted region led to open reading frame shift, producing only a short stretch of the N-terminal peptide without all functional domains of POLR2A. As a result, POLR2A expression was significantly reduced in the POLR2Aloss cells (). The POLR2Aloss and POLRZAHC'ltral HCT116 cells exhibited similar proliferation rates (), indicating that one allele of POLR2A is sufficient to maintain cell eration and survival. However, hemizygous deletion of POLR2A dramatically ized HCT116 cells to nitin ent with an IC50 of ~ 0.1 ug ml'l, which is 8- fold lower than that of parental HCT116 cells (FIGS. 2G and 2H). As a control, no substantial difference on their sensitivity to actinomycin D was observed (). Result of direct competition assays demonstrated that knockdown of POLR2A resulted in markedly reduced proliferation in the POLR2Aloss cells, but not in the isogenic POLRZAHC'ltral cells (). POLR2Aloss and POLR2Aneutra1HCT116 cells sing Dox-inducible POLR2A shRNA were also generated. Increased concentrations of Dox gradually reduced POLR2A expression. While a low-dose of Dox (100 ng ml'l) was sufficient to remarkably kill the POLR2Aloss cells, high doses of Dox only had minimal effects on the POLR2AHC'ltral cells (FIGS. 21, 2J, and 9H).
Example 3 - The sensitivity of POLR2Aloss cells to POLR2A inhibition is ndent of ] Blockage of RNA polymerase may lead to p53 accumulation and activation (Derheimer et al., 2007). To examine the effects of p53 on cell response to POLR2A inhibition, the concomitant deletion of TP53 and POLR2A was recapitulated in HCT116 and xhCRC cell lines (FIGS. 3A and 10A-10C). The xhCRC cell line (TP53+/+;POLR2A+/+) was established from a freshly isolated xenografted human colorectal tumor, which demonstrated enhanced tumorigenicity in viva (Lu et al., 2013).
Except for slightly sed cell proliferation, no significant changes in their sensitivity to 0t- Amanitin were observed in both xhCRC and HCT116 cells with hemizygous deletion of TP53. By contrast, hemizygous loss of POLR2A markedly sensitized these cells to 0t- Amanitin treatment less of their TP53 status (FIGS. 3B, 3C, and 10D-10F). The RNA polymerase II is in charge of mRNA synthesis, an essential function for any type of cells including therapy-resistant tumor cells. The drug sensitivity of POLR2Ane'ltral and POLR2Aloss cells to three major chemotherapy drugs for colorectal cancer (5-?uorouracil (5- FU), oxaliplatin, and SN-38) was examined. Inhibition of POLR2A by (x-Amanitin icantly enhanced cell-killing s of all three drugs in the POLR2Aloss xhCRC cells, but had no notable effects on the POLR2AHC'ltral cells (), suggesting therapeutic ability of POLR2Aloss colorectal tumors in cancer therapy. Free (x-Amanitin is toxic to liver because it is specifically bound by OATP1B3, a orter exclusively expressed on the membrane of hepatocytes (Letschert et al., 2006). r, (x-Amanitin, when conjugated with specific antibodies, is no longer a substrate for OATP1B3 (Letschert et al., 2006; hauer et al., 2012; Faulstich and Fiume, 1985). This gy overcomes the toxicity of (x-Amanitin for clinical applications. (x-Amanitin conjugated to a monoclonal antibody (HEA125) against EpCAM (ama-HEA125), a cancer antigen overexpressed in the ty of adenocarcinomas (Moldenhauer et al., 2012; Went et al., 2004), was used. The ama-HEA125 conjugate selectively killed the POLR2Aloss xhCRC and HCT116 cancer cells in a p53- independent manner and reduced the effective doses of (x-Amanitin by at least 10,000-fold (ICso ~ 0.01 ng ml'l) in vitro (FIGS. 3E and 10G).
Example 4 - Suppression of POLRZA selectively inhibits POLRZAIOSS tumor growth To test the anti-tumor effect of POLR2A inhibition in vivo, HCT116 and SNU283 cells expressing Dox-inducible POLR2A shRNA were ed subcutaneously into immunocompromised SCID BALB/C mice. Following initial tumor ishment, administration of Dox in drinking water suppressed POLR2A expression and consequently inhibited the growth of SNU283-derived tumors (). However, no substantial differences were observed between control and POLR2A-knockdown HCT116-derived tumors. Histopathologic analyzes demonstrated that -knockdown (Dox: 1.0 pg ml" 1) SNU283 tumors had significantly reduced cell proliferation (Ki67 staining), but more apoptotic cells (cleaved caspase-3 staining), as compared with the corresponding control tumors (FIGS. 11A-11B). By contrast, no significant changes were ed in the control or POLR2A-knockdown HCT116 . However, heterozygous deletion of POLR2A ized POLR2A+/— HCT116-derived tumors to the suppression of Dox-inducible POLR2A shRNA (C). Next, an orthotopic tumor model was employed to test the effect of POLR2A inhibition. POLR2AHC'ltral and POLR2Aloss HCT116 cells were injected into the cecal wall of SCID mice. Once orthotopic tumors were established, mice were strated Dox (1.0 pg ml'l) in the drinking water. In vivo bioluminescent imaging of tumors demonstrated that Dox-induced POLR2A inhibition led to a significant decrease in tumor growth kinetics in the POLR2Aloss tumors, but not in the control HC'ltral tumors (FIGS. 4B-C). After 3-week ent of Dox, gross tumors were visible in the POLR2AHC'ltral tumor group, whereas significantly reduced tumor growth or no visible tumor was observed in the POLR2Aloss group. To further test the efficacy of POLR2A silencing in vivo, a posomal delivery rm, DOPC (1,2-dioleoyl-sn-glycero phosphatidylcholine), was used for systemic delivery of POLR2A siRNAs (Pecot et al., 2014). Two specific POLR2A siRNAs had greater than 80% knockdown of POLR2A protein in isogenic HCT116 cell lines (A). Ten days following orthotopic injection of 1.0 x 106 isogenic HCT116 cells, mice were randomly ed to the treatment groups (n = 10 mice/group). weekly treatment of siRNAs incorporated into DOPC nanoliposomes was commenced (B). ing four weeks of systemic therapy, compared with control siRNA-DOPC treatment, mice in the 125 pg kg"1 of POLR2A siRNA-DOPC treatment groups had pronounced growth reduction of POLR2Aloss tumors, while POLR2AHC'ltral tumors only had significant growth inhibition at the dose of 1,000 pg kg"1 of POLR2A siRNA-DOPC (FIGS. 12C-F).
The ama-HEA125 conjugate exhibited a high level of selectivity on the POLR2Aloss cancer cells in vitro (FIGS. 3E and 10G). Next, the anti-tumor activity of ama- HEA125 in orthotopic tumor models established by isogenic pairs of TP5 3/POLR2AHC'ltral and TP53/POLR2Aloss xhCRC and HCT116 cells was investigated (FIGS. 4D-4E and 13A-13B).
In a dose tion experiment, ama-HEA125 was administered to the tumor-bearing mice as dual intraperitoneal injections at the dose of 3, 10, 30, or 90 ug with respect to (x-Amanitin per kg of mouse body weight (n = 10 mice per group). Control mice received unconjugated HEA125 mAb (n = 10 mice). In the mice bearing POLR2Aloss tumors, control HEA125- treated mice showed uous tumor growth within 25 days after antibody injection. The ent of ama-HEA125 showed strong inhibition on tumor growth even at the lowest dose of 3 ug kg'l. All the loss tumors responded to the ama-HEA125 treatment, and tumor volume sed dramatically (FIGS. 4D-4E). Complete tumor regression was ed in of 10 (90 ug kg'l), 8 of 10 (30 ug kg'l), and 6 of 10 (10 ug kg'l) mice bearing POLR2Aloss HCT116 tumors 25 days after the first administration of ama-HEA125 (). By contrast, significant tumor tion was observed in the mice bearing POLR2AHC'ltral tumors only at the highest dose of 90 ug kg'l, but not at the doses of 3-30 ug kg'l. Similar results were observed in the mice bearing xhCRC-derived tumors (). Consistent with previous studies nhauer et al., 2012), treatment of ama-HEA125 at the tested doses had no notable toxicity in viva. Analysis of body weights and blood liver enzymes did not reveal any substantial differences between the ama-HEA125-treated group and the control HEA125-treated group (FIGS. 13C and 13D), suggesting negligible systemic ty of the ama-HEA125 conjugate. In addition, the anti-tumor activity of ama-HEA125 was further tested in orthotopic tumors derived from the POLR2AHC'ltral SW480 cells and the POLR2Aloss SW837 cells (FIGS. F). Treatment of ama-HEA125 at a dose of 10 ug kg"1 was sufficient to inhibit tumor growth in all the POLR2Aloss tumors, while POLR2Ane'ltral ) or POLR2A-reintroduced (SW837 expressing exogenous POLR2A) POLR2Aloss tumors were only significantly inhibited by ama-HEA125 at the dose of 90 ug kg'l.
All of the s disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations may be applied to the methods and in the steps or in the sequence of steps of the method described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be nt that certain agents which are both chemically and physiologically related may be tuted for the agents described herein while the same or similar results would be achieved. All such r substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.
REFERENCES The ing references, to the extent that they provide exemplary procedural or other details supplementary to those set forth herein, are specifically incorporated herein by reference.
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Claims (17)

1. Use of a POLR2A inhibitor in the manufacture of a medicament for the ent of a cancer, wherein cells of the cancer have been determined to comprise (i) a hemizygous loss of the TP53 gene; (ii) a hemizygous loss of the POLR2A gene; or (iii) a sed level of expression of a POLR2A gene product relative to a reference level, wherein the POLR2A inhibitor comprises an amatoxin, and wherein the amatoxin is ated to an antibody which specifically binds to a tumor-associated antigen, wherein said medicament comprises a therapeutically ive amount of said POLR2A inhibitor.
2. The use according to claim 1, wherein the amatoxin is alpha-amanitin.
3. The use according to claim 1, n the POLR2A gene product is an mRNA.
4. The use according to claim 3, wherein the level of expression of the mRNA is determined by Northern blotting, reverse transcription-quantitative real-time PCR (RT- qPCR), se protection, transcriptome analysis, a hybridization assay, a chip-based expression platform, or an r RNA assay platform.
5. The use according to claim 1, wherein the POLR2A gene product is a protein.
6. The use according to claim 5, wherein the level of expression of the protein is determined by mass spectrometry, western blot, ELISA, immunoprecipitation, immunohistochemistry, or radioimmunoassay.
7. The use according to claim 1, wherein the reference expression level is an expression level in non-cancerous cells.
8. The use according to claim 1, wherein the hemizygous loss of the TP53 gene or the gous loss of the POLR2A gene is determined by a genomic hybridization technique, PCR analysis, or restriction fragment analysis.
9. The use according to claim 1, wherein the cancer cells exhibit a hemizygous loss of the TP53 gene and/or a hemizygous loss of the POLR2A gene.
10. The use ing to claim 1, wherein the cancer cells exhibit a sed level of expression of a POLR2A gene product relative to a reference expression level.
11. The use according to claim 1, wherein the cancer cells are lung cancer, brain , breast cancer, liver cancer, ovarian cancer, colorectal cancer, te cancer, or pancreatic cancer cells, and/or wherein the cancer cells are atic, ent, or multi-drug resistant.
12. The use according to claim 1, wherein the medicament further comprises at least a second anticancer therapeutic, preferably wherein the second anticancer therapeutic is a chemotherapeutic agent, and more preferably n the chemotherapeutic agent is 5-fluorouracil, oxaliplatin, or SN-38.
13. The use according to claim 1, wherein the cancer is in a patient that has previously undergone at least one round of anti-cancer therapy.
14. The use according to claim 1, wherein the treatment of a cancer further comprises at least a second anticancer therapy, ably wherein the second anticancer therapy is a al therapy, herapy, radiation therapy, cryotherapy, hormonal therapy, toxin therapy, immunotherapy, or cytokine therapy.
15. An in vitro method of selecting a drug therapy for a cancer patient comprising: (a) providing a sample of said cancer previously obtained from said patient; (b) detecting the presence of (i) a hemizygous loss of the TP53 gene; (ii) a gous loss of the POLR2A gene; or (iii) a decreased level of expression of a POLR2A gene product relative to a reference level in cells of the cancer; and (c) selecting a POLR2A inhibitor if (i) a hemizygous loss of the TP53 gene is detected; (ii) a hemizygous loss of the POLR2A gene is detected; or (iii) a decreased level of expression of a POLR2A gene product relative to a nce level is detected in cells of the cancer, wherein the POLR2A inhibitor ses an amatoxin, and wherein the amatoxin is conjugated to an antibody which specifically binds to a tumorassociated antigen.
16. The method of claim 15, wherein cells of the cancer comprise a hemizygous loss of the TP53 gene and/or a hemizygous loss of the POLR2A gene.
17. The method of claim 15, n cells of the cancer comprise a decreased level of expression of a POLR2A gene product relative to a reference level. nocmnmg 6?u% r0«U% 5:533 3andwm Ch??? $§NP3--+ "n’ ...."up "up ‘nnnn POLRRA 2 SHBG T953 EGQKb VEGA Gama {:3mg?} ?.nu §u mu9».A .ba“mm T:p. ,3Qu RVan wm Hemkygmw tie¥atim COLE (“:3 343) TCGA scion {nz395} § 6.8 - s E3 ‘3 6.5 $3 @255 N 3 g3 5.5 'V :3. E q} 0.... I} g 45 g; g 9 $3 3.5 3 4.5 m (B X :3: § 2.5 g 4.0 ~12 ~38 ~84 9,0 0.4 ~10 4.5 ~19 «(3.5 0.6 0.5 m GNA copy Rumba? {Log2 ratio} DNA cepy number {Logz ratio} : p <1 {3.885 0 2.8 W 32* m Eta is a: 3-. fr: a. g {3.8 ?eutrai Loss “3&3”; 2.3 $3 is am um?aaam a; am an.»5 ¥3GLR2A 4: RN «2% s: .3 ag?am an5 {:«Amaniiia {gngfi’?} \E§$§§$§w?%%%‘33,. {av _. a Q. Q Q} {2}“ \. M\ \\ Absarbance SNL¥ 283 m MM ssw 83'? ..... \ - MM SW £386 ... MS"? as Relative m?xmaai?n {gym} ‘ SS3233 Pmiiferaii?n W POLRZé mRN?x 12 ‘3)? 1.3 § * 3 x 9.3 0 at ‘3 § 3: {L8 g 3 9.4 .2 ‘3 E - {3.2 g Q ”3 “$3 rt?,§@“(ngimi} HCT‘338 SNUESS SW83? W 1.8 pg 8 “W“ {3.5 §3§ g. ‘3 MM {3.25 gg § ‘33 MM»: 8?? 3 M "" Ram ......... <‘M‘ M “ g 3.2 ?§§%§‘§§a§§’ \M‘?‘ mkmanitin {yg?mi} «MM -3% ”LE §.¥.§- g \\- 8’5 i ‘3 §v§g 3 MM £3.25 ggg {3 g ..... .a ;. a» BMW .3 {3x2 ........... EE-
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