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NZ749473B2 - Benzo-n-hydroxy amide compounds having antitumor activity - Google Patents
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NZ749473B2 - Benzo-n-hydroxy amide compounds having antitumor activity - Google Patents

Benzo-n-hydroxy amide compounds having antitumor activity Download PDF

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Publication number
NZ749473B2
NZ749473B2 NZ749473A NZ74947317A NZ749473B2 NZ 749473 B2 NZ749473 B2 NZ 749473B2 NZ 749473 A NZ749473 A NZ 749473A NZ 74947317 A NZ74947317 A NZ 74947317A NZ 749473 B2 NZ749473 B2 NZ 749473B2
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New Zealand
Prior art keywords
phenyl
hydroxycarbamoyl
amino
propanamide
propenoyl
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NZ749473A
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NZ749473A (en
Inventor
Gianluca Fossati
Maria Lattanzio
Paolo Mascagni
Daniela Modena
Pietro Samuele Pozzi
Giovanni Sandrone
Christian Steinkuhler
Andrea Stevenazzi
Barbara Vergani
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Italfarmaco Spa
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Priority claimed from IT102016000074606A external-priority patent/IT201600074606A1/en
Application filed by Italfarmaco Spa filed Critical Italfarmaco Spa
Publication of NZ749473A publication Critical patent/NZ749473A/en
Publication of NZ749473B2 publication Critical patent/NZ749473B2/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/16—Amides, e.g. hydroxamic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/16—Amides, e.g. hydroxamic acids
    • A61K31/165—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/357—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having two or more oxygen atoms in the same ring, e.g. crown ethers, guanadrel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/357—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having two or more oxygen atoms in the same ring, e.g. crown ethers, guanadrel
    • A61K31/36—Compounds containing methylenedioxyphenyl groups, e.g. sesamin
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A61K31/403—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
    • A61K31/4035—Isoindoles, e.g. phthalimide
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    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/438—The ring being spiro-condensed with carbocyclic or heterocyclic ring systems
    • A—HUMAN NECESSITIES
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    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4406—Non condensed pyridines; Hydrogenated derivatives thereof only substituted in position 3, e.g. zimeldine
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    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
    • A61K31/443—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with oxygen as a ring hetero atom
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    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • A61P35/04—Antineoplastic agents specific for metastasis
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    • C07C259/04—Compounds containing carboxyl groups, an oxygen atom of a carboxyl group being replaced by a nitrogen atom, this nitrogen atom being further bound to an oxygen atom and not being part of nitro or nitroso groups without replacement of the other oxygen atom of the carboxyl group, e.g. hydroxamic acids
    • C07C259/10—Compounds containing carboxyl groups, an oxygen atom of a carboxyl group being replaced by a nitrogen atom, this nitrogen atom being further bound to an oxygen atom and not being part of nitro or nitroso groups without replacement of the other oxygen atom of the carboxyl group, e.g. hydroxamic acids having carbon atoms of hydroxamic groups bound to carbon atoms of six-membered aromatic rings
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    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
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    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/24—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D213/54—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D213/56—Amides
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    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/62—Oxygen or sulfur atoms
    • C07D213/63—One oxygen atom
    • C07D213/64—One oxygen atom attached in position 2 or 6
    • C07D213/643—2-Phenoxypyridines; Derivatives thereof
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    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
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    • C07D215/12—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D215/14—Radicals substituted by oxygen atoms
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    • C07D221/02—Heterocyclic compounds containing six-membered rings having one nitrogen atom as the only ring hetero atom, not provided for by groups C07D211/00 - C07D219/00 condensed with carbocyclic rings or ring systems
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    • C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
    • C07D233/64—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with substituted hydrocarbon radicals attached to ring carbon atoms, e.g. histidine
    • C—CHEMISTRY; METALLURGY
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    • C07D317/02—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 2
    • C07D317/06—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 2 condensed with carbocyclic rings or ring systems
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    • C07D317/08—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3
    • C07D317/44—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3 ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D317/46—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3 ortho- or peri-condensed with carbocyclic rings or ring systems condensed with one six-membered ring
    • C07D317/48—Methylenedioxybenzenes or hydrogenated methylenedioxybenzenes, unsubstituted on the hetero ring
    • C07D317/50—Methylenedioxybenzenes or hydrogenated methylenedioxybenzenes, unsubstituted on the hetero ring with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to atoms of the carbocyclic ring
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Abstract

The present invention relates to new benzo-N-hydroxy amide compounds of formula (I) and pharmaceutically acceptable salts thereof, which show a significant inhibitory activity on the proliferation of tumor cells and specifically of cancer stem cells.

Description

Title BENZO-N-HYDROXY AMIDE COMPOUNDS HAVING ANTITUMOR ACTIVITY Description Field of the invention The present invention relates to new benzo-N-hydroxy amide compounds having an alpha- amino acidic scaffold carrying an alpha-beta unsaturated yl moiety on the amino-group and to the pharmaceutical compositions thereof.
The molecules have antitumor ty and are ularly active on cancer stem cells.
Background of the invention Partial or even complete cancer regression can be achieved in some patients with current cancer treatments. However, such initial responses are almost always followed by relapse, with the recurrent cancer being resistant to further ents. The discovery of therapeutic approaches that counteract relapse is, therefore, essential for advancing cancer medicine.
Cancer cells are ely heterogeneous, even in each individual patient, in terms of their malignant ial, drug sensitivity, and their potential to metastasize and cause relapse.
Indeed, hypermalignant cancer cells, termed cancer stem cells or tumor-initiating cells, that are highly tumorigenic and metastatic have been isolated from cancer patients with a variety of tumor types. er, such stemness-high cancer cells are resistant to conventional chemotherapy and radiation.
Therefore, development of specific therapies targeted at cancer stem cells holds hope for improvement of survival and y of life of cancer patients, especially for patients with metastatic disease.
US7635788 ses hydroxamic acid derivatives containing an alpha-aminoacyl moiety and having inhibitory activity on the proinflammatory cytokines production, in particular TNFG.
Such compounds are useful in the treatment of inflammatory es and other disorders characterised by overproduction of TNFOt or other proinflammatory cytokines. Said compounds furthermore exhibit a cytotoxicity activity in in vitro testing on human hepatoma cell line Hep-G2.
Definitions Unless otherwise defined, all terms of art, notations and other scientific terminology used herein are intended to have the meanings commonly understood by those of skill in the art to which this disclosure pertains. In some cases, terms with commonly understood gs are defined herein for clarity and/or for ready nce; thus, the inclusion of such definitions herein should not be construed to represent a substantial difference over what is generally understood in the art.
The term "halogen" herein refers to fluorine (F), ne (Cl), bromine (Br), or iodine (I).
The term “C1-C4 alkyl” herein refers to a branched or linear hydrocarbon containing from 1 to 4 carbon atoms. Examples of C1-C4 alkyl groups e but are not limited to methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl.
The term “aryl” herein refers to aromatic mono- and poly-carbocyclic ring s, wherein the individual carbocyclic rings in the poly-carbocyclic ring systems may be fused or attached to each other via a single bond. Suitable aryl groups include, but are not limited to, phenyl, naphthyl and biphenyl.
The term "aryloxy" herein refers to O-aryl group, wherein "aryl" is defined above.
The term "alkoxy" herein refers to O-alkyl group, wherein "alkyl" is defined above.
The term “arylalkyl” herein refers to an aryl radical, as d herein, attached to an alkyl l, as defined herein.
The term “arylcarbonyl” herein refers to —C(O)—aryl, wherein "aryl" is defined above.
The term “heterocycle” herein refers to a 4-, 5-, 6-, 7- or 8-membered monocyclic ring which is ted or unsaturated, and which consists of carbon atoms and one or more heteroatoms selected from N, O and S, and wherein the nitrogen and sulphur heteroatoms may optionally be oxidized, and the nitrogen heteroatom may ally be quaternized. The heterocycle ring may be attached to any heteroatom or carbon atom, provided that attachment results in the creation of a stable structure. The term also includes any bicyclic system in which any of the above heterocyclic rings is fused to an aryl or another heterocycle. When the heterocycle ring is an aromatic heterocycle ring it can be defined “heteroaromatic ring”.
The terms “comprising”, “having”, “including” and “containing” are to be construed as open- ended terms (i.e. meaning “including, but not d to”) and are to be considered as providing support also for terms as “consist essentially of”, “consisting essentially of”, “consist of” or sting of”.
The terms “consist essentially of”, “consisting essentially of” are to be construed as a semi- closed terms, meaning that no other ingredients and/or steps which materially affects the basic and novel characteristics of the invention are included (optional excipients may be thus included).
The terms “consists of”, “consisting of” are to be construed as a closed term.
The term “pharmaceutically acceptable salts” herein refers to those salts which possess the biological effectiveness and properties of the salified compound and which do not produce adverse reactions when stered to a mammal, ably a human. The pharmaceutically acceptable salts may be inorganic or organic salts; es of pharmaceutically acceptable salts include but are not d to: carbonate, hydrochloride, hydrobromide, sulphate, hydrogen sulphate, citrate, e, fumarate, acetate, trifluoroacetate, e, 2- naphthalenesulphonate, and para-toluenesulphonate. r information on pharmaceutically acceptable salts can be found in Handbook of pharmaceutical salts, P. Stahl, C. Wermuth, WILEY-VCH, 127-133, 2008, herein incorporated by reference.
The term “physiologically acceptable excipient” herein refers to a substance devoid of any pharmacological effect of its own and which does not produce adverse reactions when administered to a mammal, preferably a human. Physiologically acceptable excipients are well known in the art and are disclosed, for ce in the Handbook of Pharmaceutical ents, sixth edition 2009, herein orated by reference.
The term “isomers” herein refers to structural (or constitutional) isomers (i.e. tautomers) and stereoisomers (or l isomers) i.e diastereoisomers and enantiomers. ption of the ion It has now been found that benzo-N—hydroxy amide compounds having an alpha—amino acidic ld carrying an alpha—beta unsaturated carbonyl moiety on the amino—group show a high inhibitory activity on the proliferation of tumor cells and of staminal tumor cells. These compounds are particularly active on cancer stem cells, making them useful in the treatment of human neoplasia.
The present invention provides compounds of the formula (1): O ( R4 n H \ \ \ N f N o n ‘x/ \OH and pharmaceutically acceptable salts, isomers and prodrugs thereof, wherein: n is 0, l or 2; A is absent or is a mono or di—carbocyclic residue, optionally partially or totally unsaturated, comprising carbon atoms and optionally one or more heteroatoms selected from N, S or O; SUBSTITUTE SHEET (RULE 26) R1 and R2 are independently selected from the group comprising –H, –OH, -OMe, -CN, -NH2, -NO2, -Cl, -COOH, -halogen, -CF3, -N(Ra)2, linear or branched C1-C4 alkyl, aryl, arylalkyl, arylcarbonyl, alkoxy, aryloxy residue, nylamino and -CH2N(CH2CH3)2; Ra is a linear or ed C1-C3 alkyl residue; X can be C or N; R3 and R4 are independently selected from the group comprising –H, -OMe, -OPh, -NO2, - NMe2, -NH2, -halogen, -CF3, -N(Ra)2, linear or branched C1-C4 alkyl, aryl, arylalkyl, rbonyl, alkoxy, aryloxy residue and sulphonylamino, or R3 and R4 together can form a heteropentacyclic moiety (-OCH2O-).
In a particular aspect, the present invention provides a compound of the formula (I) and pharmaceutically acceptable salts and stereoisomers thereof, wherein: n is 0, 1 or 2; A is H, provided that when A is H, R1 and R2 are absent, or is a mono or di-carbocyclic residue, optionally lly or totally unsaturated, comprising carbon atoms and optionally one or more heteroatoms selected from N, S or O; R1 and R2 are independently selected from the group consisting of –H, –OH, -OMe, -CN, - NH2, -NO2, -Cl, -COOH, -halogen, -CF3, -N(Ra)2, linear or branched C1-C4 alkyl, aryl, arylalkyl, rbonyl, alkoxy, y residue, sulphonylamino and -CH2N(CH2CH3)2; Ra is a linear or branched C1-C3 alkyl residue; [FOLLOWED BY PAGE 5a] X is C or N; R3 and R4 are independently selected from the group consisting of –H, -OMe, -OPh, -NO2, - NMe2, -NH2, en, -CF3, -N(Ra)2, linear or branched C1-C4 alkyl, aryl, arylalkyl, arylcarbonyl, , aryloxy residue and nylamino, or R3 and R4 together form a heteropentacyclic moiety comprising (-OCH2O-).
The compounds of the present invention can exist in different isomeric forms: the cis-form and the trans-form.
The preferred isomeric form is the trans-form.
The compounds of the invention contain one or more chiral centers (asymmetric carbon atoms) and may thus exist in enantiomeric and/or reoisomeric forms; all possible optical isomers, alone or mixed with one another, fall within the scope of the present invention. gs of compounds of formula (I) are included in this ion. Such prodrugs are bioreversible derivatives of compounds (I). They are pharmacologically inactive derivatives, which can undergo in vivo metabolic transformation to afford an active compound included in the general a of this invention. Many different prodrugs are known in the art [Prodrug approach: an effective solution to overcome side-effects, Patil S.J., Shirote P.J., International Journal of Medical and Pharmaceutical Sciences, 2011,1-13; ate Prodrug Concept for Hydroxamate HDAC Inhibitors, Jung, Manfred et al., ChemMedChem, 2011, 1193-1198].
Some compounds of a (I) present one or more basic or acidic moieties, which can undergo salification by tional chemical methods, generally treatment of the compounds with organic or inorganic acid, organic or inorganic base, or by ion-exchange [FOLLOWED BY PAGE 6] chromatography. Examples of pharmaceutically acceptable salts include, but are not limited to, those deriving from nic acids such as hydrochloric, hydrobromic, sulfuric, phosphoric, nitric and the like, or from organic acids such as acetic, maleic, propionic, succinic, glycolic, stearic, malic, tartaric, citric, ascorbic, pamoic and the like. Further examples of pharmaceutically able salts include, but are not d to, those deriving from bases such as aluminium, um, calcium, copper, ferric, lithium, magnesium, potassium, sodium, zinc and the like. Pharmaceutically acceptable salts are well known in the art. Their preparation is well described by Berg et al. in “Pharmaceutica salts”, J. Pharm. Sci., 1977, 66, 1-19.
One class of preferred compounds comprises nds of the formula (I) and pharmaceutically acceptable salts, isomers and prodrugs f, in which: n is 0, l or 2; A is absent or is a mono or di-carbocyclic residue, optionally partially or totally unsaturated, comprising carbon atoms and optionally one or more heteroatoms selected from N, S or O; R1 and R2 are independently selected from the group comprising —H, —OH, -OMe, -CN, -NH2, -NOz, -Cl, -COOH and -CH2N(CH2CH3)2; X can be C or N; R3 and R4 are independently selected from the group comprising —H, -OMe, -OPh, -NOz, - NMez and -NH2, or R3 and R4 together can form a heteropentacyclic moiety (-OCHzO-).
Preferably, A is absent or is a mono or di-carbocyclic residue ed from the group comprising phenyl, naphthyl, pyridyl, indanyl, quinolyl, imidazolyl, indolyl, thiazolyl, benzothiophenyl. r class of more preferred compounds ses nds of the formula (I) and pharmaceutically acceptable salts, isomers and prodrugs thereof in which: n is l, X is C; R1 and R2 are independently —H, -Cl or —OMe; R3 and R4 are independently —H, -NMe2, or R3 and R4 together can form a heteropentacyclic moiety (-OCHzO-).
The following compounds of the formula (I) are particularly preferred: - (28)[[(E)(l,3-benzodioxolyl)propenoyl]amino]-N-[4- xycarbamoyl)phenyl](4-methoxyphenyl)propanamide (l D) ; - (ZS)-N-[4-(hydroxycarbamoyl)phenyl](4-methoxyphenyl)[[(E)phenylprop enoyl]amino]propanamide (2D); - -[[(E)(l,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-propanamide (4D); - (ZS)-N- [4-(hydroxycarbamoyl)phenyl]-3 -phenyl [ [(E)-3 -phenylprop enoyl]amino]propanamide (5D); - (ZS)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](4-hydroxyphenyl)propanamide (7D); - (E)(2,5-dimethoxyphenyl)-N-[(lR)[4-(hydroxycarbamoyl)anilino]-l-indanyl- ethyl]propenamide (8D); - (ZS)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-butanamide (9D); - (ZS)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-propanamide (10D); - (ZS)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](2-naphthyl)propanamide (1 1D); - (E)-3 -(2,5 -dimethoxyphenyl)-N- [2- [4-(hydroxycarbamoyl)anilino]oxo-ethyl]prop- 2-enamide (12D); - (28)[[(E)(2,5 -dimeth0xypheny1)pr0p—2-en0y1]amin0]—N- [4- (hydroxycarbam0y1)pheny1]pr0panamide (13D); - (28)[[(E)(2,5 -dimeth0xypheny1)pr0p—2-en0y1]amin0]—N- [4- (hydroxycarbam0y1)phenyl](3-quin01y1)pr0panamide (14D); - (28)[[(E)(2,5 -dimeth0xypheny1)pr0p—2-en0y1]amin0]—N- [4- (hydroxycarbam0y1)phenyl](3-pyridy1)pr0panamide (15D); - (28)[[(E)(2,5 -dimeth0xypheny1)pr0p—2-en0y1]amin0]—N- [4- (hydroxycarbam0y1)phenyl](4-nitrophenyl)pr0panamide (16D); - (ZS)[[(E)(4-aminophenyl)pr0pen0y1]amin0]-N- [4- (hydroxycarbam0y1)phenyl]pheny1—pr0panamide (17D); - (ZS)(4-amin0phenyl)[[(E)-3 -(2,5 -dimeth0xyphenyl)pr0pen0y1]amin0]-N- [4- (hydroxycarbam0y1)pheny1]pr0panamide (18D); - -(4-cyan0pheny1)[[(E)(2,5-dimeth0xyphenyl)pr0pen0y1]amin0]-N-[4- (hydroxycarbam0y1)pheny1]pr0panamide (19D); - (ZS)-N- [4-(hydroxycarbam0y1)pheny1] [ [(E)-3 -(4-nitr0pheny1)propen0y1] amino]pheny1—pr0panamide (20D); - (28)[[(E)(2,5 h0xypheny1)pr0p—2-en0y1]amin0]—N- [4- (hydroxycarbamoy1)pheny1] -3 -(1H-imidaz01—5 opanamide (21D); - - [ [(E)-3 -(3 ,4-dimeth0xyphenyl)pr0pen0y1]amin0]—N- [4- (hydroxycarbam0y1)phenyl](3-pyridy1)pr0panamide (22D); - (ZS) [ [(E)-3 -(3 ,5 -dimeth0xypheny1)pr0p—2-en0y1]amin0]—N- [4- (hydroxycarbam0y1)phenyl](3-pyridy1)pr0panamide (23D); - (28)[[(E)(1,3 -benz0di0x01—5-y1)pr0pen0y1]amin0]-N- [4- (hydroxycarbam0y1)phenyl](3-pyridy1)pr0panamide (24D); - (ZS)[[(E)(1,3 -benz0di0x01—5-y1)pr0pen0y1]amin0](benz0thi0phenyl)-N- [4-(hydroxycarbam0y1)phenyl]pr0panamide (25 D); - (ZS)[[(E)(1,3 -benz0di0x01—5-y1)pr0pen0y1]amin0]-N- [4- (hydroxycarbam0y1)pheny1]thiaz01—4-y1—pr0panamide (26D); - (ZS)[[(E)(1,3 di0x01—5-y1)pr0pen0y1]amin0]-N- [4- (hydroxycarbam0y1)pheny1]pheny1-butanamide (27D); - (ZS) [ [(E)-3 -(3 ,4-dimeth0xypheny1)pr0p—2-en0y1]amin0]—N- [4- (hydroxycarbam0y1)pheny1]pheny1-pr0panamide (28D); - (ZS)[[(E)(1,3 -benz0di0x01—5-y1)pr0pen0y1]amin0](4-cyan0pheny1)-N-[4- (hydroxycarbam0y1)pheny1]pr0panamide (29D); - (ZS) [ [(E)-3 -(3 ,4-dimeth0xypheny1)pr0p—2-en0y1]amin0]—N- [4- (hydroxycarbam0y1)pheny1]-3 -(1H-ind01—3 -y1)pr0panamide (30D); - -[[(E)(1,3 -benz0di0x01—5-y1)pr0pen0y1]amin0]-N- [4- (hydroxycarbam0y1)pheny1]-3 -(1H-ind01—3 -y1)pr0panamide (31D); - (ZS)[[(E)(1,3 -benz0di0x01—5-y1)pr0pen0y1]amin0]-N- [4- (hydroxycarbam0y1)pheny1](4-nitrophenyl)pr0panamide (32D); - (ZS)[[(E)(1,3 -benz0di0x01—5-y1)pr0pen0y1]amin0](4-ch10r0pheny1)-N-[4- xycarbam0y1)pheny1]pr0panamide (33D); - (ZS)(4-amin0pheny1)[[(E)(3,4-dimeth0xypheny1)pr0pen0y1]amin0]-N-[4- (hydroxycarbam0y1)pheny1]pr0panamide (34D); - (ZS)(4-amin0pheny1)[[(E)(1,3-benz0di0x01—5-y1)pr0pen0y1]amin0]-N-[4- (hydroxycarbam0y1)pheny1]pr0panamide (35D); - 4-[(2$)[[(E)(1,3-benz0di0x01—5-yl)pr0pen0y1]amin0]—3-[4- (hydroxycarbamoyl)anilin0]0X0—pr0py1]benz0ic acid (36D); - (ZS)[[(E)(l ,3 dioxolyl)propenoyl]arnino]—3-(3 ,4-dichlorophenyl)-N- [4-(hydroxycarbamoyl)phenyl]propanarnide (37D); - (ZS)-N-[4-(hydroxycarbamoyl)phenyl](4-methoxyphenyl)[[(E)phenylprop enoyl]amino]propanamide (38D); - (ZS)[[(E)(4-dimethylaminophenyl)propenoyl]amino]—N-[4- (hydroxycarbamoyl)phenyl]—3-phenyl-propanamide (39D); - (ZS)[[(E)(4-dimethylaminophenyl)propenoyl]amino]—N-[4- xycarbamoyl)phenyl]—3-(4-methoxyphenyl)propanarnide; 2,2,2-trifluoroacetic acid (40D); - (E)-N-[2-[[4-(hydroxycarbamoyl)phenyl]methylamino]oxo-ethyl]phenyl-prop enamide (41D); - (E)(l,3-benzodioxolyl)-N-[2-[[4-(hydroxycarbamoyl)phenyl]methylamino] oxo-ethyl]propenamide (43 D); - (ZS)-3 - [4-(diethylaminomethyl)phenyl]-N- [4-(hydroxycarbamoyl)phenyl] [ [(E)-3 - phenylpropenoyl]amino]propanamide; 2,2,2-trifluoroacetic acid (45D); - (28)[[(E)(l,3-benzodioxolyl)propenoyl]arnino]—3-[4- (diethylaminomethyl)phenyl]-N-[4-(hydroxycarbamoyl)phenyl]propanamide; 2,2,2- trifluoroacetic acid (46D); - (ZS)-N-[4-(hydroxycarbamoyl)phenyl](2-naphthyl)[[(E)(6-phenoxy pyridyl)propenoyl]amino]propanamide (47D).
The following nds of the formula (I) are more particularly preferred: - (28)[[(E)(l,3-benzodioxolyl)propenoyl]arnino]—N-[4- (hydroxycarbamoyl)phenyl]—3-(4-methoxyphenyl)propanamide (l D) ; - (ZS)-N-[4-(hydroxycarbamoyl)phenyl](4-methoxyphenyl)[[(E)phenylprop enoyl]amino]propanamide (2D); - (28)[[(E)(l,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-propanamide (4D); - (ZS)-N- [4-(hydroxycarbamoyl)phenyl]-3 -phenyl [ [(E)-3 lprop enoyl]amino]propanamide (5D).
The compounds of the invention may be prepared using methods known to the person skilled in the art.
All starting als, building blocks, reagents, acids, bases, solvents and catalysts used to synthesize nds of the present invention are commercially available.
Compounds of a (I) can be prepared both by solid phase synthesis (scheme 1) and by solution synthetic method (scheme 2). In some cases it could be necessary to synthesize the compound in a protected species and to add one or more step for the de-protection.
Reactions are red by HPLC or LC-MS analysis.
Final products are generally purified by preparative HPLC-MS chromatography or by SPE on reverse phase filled cartridge.
Solid Phase Smthesis Compounds of formula (I) can be sised by SPS following scheme 1.
N-fmocaminobenzoic acid is loaded on hydroxylamine Wang resin after classical tion with HATU, HOAt and DIPEA. The Fmoc-deprotection is performed by treating the resin with piperidine 20% solution in DMF for 15 minutes. A fmoc-amino acid is then coupled to the aromatic amine, after activation with HATU, HOAt and DIPEA. Another fmoc-deprotection step follows and finally the amine group is acylated by treatment of the resin with an activated cinnamic acid tive. Cleavage of the product from the resin is obtained by treatment with TFA 50% solution in DCM.
Final products are usually purified by preparative LC-MS chromatography or by SPE on C18 filled cartridge.
Scheme 1 HATU O 1‘ pipendine 20% H0 #31:: DMF fl Fmoc / Fmoc 1 g N/ R 2‘ a H R“ Fmoc OH H o HATU; HOAt DIPEA, DMF 1‘ plpendine 20%. . . in DMF X R O N O / E R4 0 H 2 H l N N \Fmoc WOH \ \ H R3 X E R4 ) HATU HOAt R n , ) o DIPEA, DMF n R‘ K1 IFA 50% in DC fl Solution Phase Sflthesis Compounds of formula (D can also be prepared by cal solution synthesis, as shown in scheme 2. The ion of the amide can be performed using one of the method known to one of skill in the art. For example, after activation of carboxylic moiety with HATU and DIPEA, a Fmoc-amino acid is reacted with ethyl—4-aminobenzoate. The obtained compound A is deprotected by treatment with piperidine 20% in DMF and then acylated by on with activated ic acid derivative to give the intermediate B. The final hydroxamic compound can be obtained following different synthetic route. It is possible to perform a direct hydroxylaminolysis by treatment of the ester intermediate with hydroxylamine and NaOH in methanol.
SUBSTITUTE SHEET (RULE 26) Otherwise it is possible to hydrolyse the ester function with NaOH and then to transform the obtained carboxylic acid in hydroxamic acid by reaction with hydroxylamine after activation via HATU.
In some cases it is preferably to transform the carboxylic acid in a protected form of hydroxamate, in order to perform a purification of a nd easier to . In this case the final compound is obtained by deprotection with a TFA solution in Methanol.
Scheme 2 R' 1. piperidine 20% R 1 in DMF R“ R4 o ( liHATU ( 2 W0” n DlPEA RzX Fmoo El Fmoc\ on k \N HATU, MF N H —» H HEN [:HO\/ O O or O R4 O 2' \/ \ C1 O R1 X R1 R1 R3 R2 0 l o ( n R4 H MOIIIN, R4 N g THF/EtOH \ \ —> \ \ N N | H | H o 0v / 0 B 3 X Lt R: X/ R HATU, DlPEA NHZOH 50% HATU, DIPEA, NaOH 1N anon 3M, HMO00 VleOH O ( n R4 I I TFA N MeOH WU / O N \00 z R X O wherein A, R1, R2, R3, R4 and X have the same meanings reported above.
Preparative HPLC-MS chromatography SUBSTITUTE SHEET (RULE 26) The purification was performed using a Waters preparative HPLC system, equipped with a mass ometer detector (ZQZOO). Three different methods have been used for purification, depending on the nature of R1, R2, R3 and R4 groups.
Crude product was dissolved in DMSO. The solution was filtered through a 0.45 um PTFE ne and injected in the preparative system. ons corresponding to the peak associated with the expected molecular ion ([M+H]+)were collected and concentrated to dryness.
Operating conditions: 0 Column: Waters SunFireTM Prep C18 OBDTM 5 m, 19X100mm 0 Solv. A H20 0 Solv. B ACN 0 Solv. C 1% formic acid in H20 HPLC method 1: Flow late Time (min) Solv.A Solv. B Solv. C (ml/min) 0 55% 35% 10% 20 45% 45% 10% 20 22 0% 90% 10% 20 24 0% 90% 10% 20 26 55% 45% 10% 20 55% 45% 10% 20 HPLC method 2: Flow late Time (min) Solv.A Solv. B Solv. C 0 70% 20% 10% 20 50% 40% 10% 20 22 0% 90% 10% 20 24 0% 90% 10% 20 26 70% 20% 10% 20 70% 20% 10% 20 HPLC method 3: Flow late Time (min) Solv.A Solv. B Solv. C (ml/min) 0 75% 15% 10% 20 55% 35% 10% 20 22 0% 90% 10% 20 24 0% 90% 10% 20 26 75% 15% 10% 20 75% 15% 10% 20 MS method: 0 Centroid ES+ ionisation, 0 Scan time 30 min, 0 m/z scan 100-1000, 0 Cone e 15V, 0 Source temperature 1500C, 0 Desolvation temperature 280 0C.
Solid Phase Extraction The purification has been performed on reverse phase pre-filled SPE cartridge (Phenomenex Strata C18-E, 55 mm, 70A).
Crude product was dissolved in DMF and loaded on the cartridge. The t was eluted with ter mixtures at different ratios, depending on the nature of R1, R2, R3 and R4 groups. Eluted fractions which showed a HPLC purity area over 85% were collected and concentrated to dryness.
The compounds of the present invention showed high inhibitory activity on the proliferation of cancer stem cells in vitro, with IC50 values of nanomolar order.
In vivo studies were also carried out, monitoring the capability of compounds to reduce tumor size and weight as described, for example, in e 7.
These nds may accordingly be used, alone or together with other antitumor drugs, in the prevention and/or treatment of cancer. 2017/067850 The compounds of the invention are preferably useful for the prevention and/or treatment of solid tumors such as colorectal cancer, lung, brain, prostate or gynecological cancers or hematologic malignancies.
The compounds of the invention are particularly active on cancer stem cells. Therefore, said compounds are ably useful for the tion and/or treatment of metastatic, ent and drug-resistant diseases.
The present invention accordingly also provides pharmaceutical compositions comprising a therapeutically effective ty of the compounds of the formula (I) or of the pharmaceutically acceptable salts, isomers and prodrugs thereof together with at least one pharmaceutically acceptable excipient.
Such compositions may be liquid, suitable for enteral or parenteral administration, or solid, for example, in the form of capsules, tablets, coated tablets, powders or granules for oral administration, or in forms suitable for ous administration, such as for example creams or ointments, or inhalatory stration.
The pharmaceutical itions provided by the present invention may be prepared using known methods.
The following examples have the purpose of further illustrating the invention without however limiting it.
EXAMPLES The abbreviations below are used in the following Examples: ACN Acetonitrile Boc tert-Butyloxycarbonyl DCM dichloromethane DEA diethylamine DIPEAN,N-diisopropylethylamine DMF dimethylformamide methylsulfoXide EtOAc ethyl acetate EtOH Ethanol Et20 diethyl ether ES Electrospray Fmoc Fluorenylmethyloxycarbonyl HATU O-(7-azabenzotriazol-yl-)-N,N,N’ ,N’ -tetramethyluronium hexafluorophosphste HOAt l-hydroxy-7 -azabenzotriazole HPLC high pression liquid chromatography LC—MS HPLC system equipped with a mass spectrometer MeOH methanol PTFE Polytetrafluoroethylene RT Room Temperature SPE solid phase extraction SPS solid phase synthesis TFA roacetic acid THF tetrahydrofurane EXAMPLE 1 (Compound 4D) Synthesis of 4-(2-(3-(benzo[d][l,3]dioxolyl)acrylamido)phenylpropanamido)-N- hydroxybenzamide WO 15292 < \ N o n 0 \OH STEP A: Ethyl (2-((((9H-fluorenyl)methoxy)carbonyl)amino) phenylpropanamido)benzoate H2N H Fmoo N Fmoo OH + O\/ —’ H N o o H \/ O O 3g of Fmoc-L-Phenylalanine (7.74 mmol, 1 eq.) were ved in 15 ml of DMF and the solution was cooled at 00C. HATU (3.84 g, 1.3 eq.) and DIPEA (1.75 ml, 1.3 eq.) were added and the reaction mixture was stirred for half an hour. Ethyl 4-aminobenzoate (1.40g, 1 eq.) was then added and the mixture was stirred at RT for 1h. The reaction was monitored by HPLC analysis. When the reaction was complete, the on was poured in water (150ml).
The white solid precipitated was filtered and dried. 4g of pure product was obtained.
STEP B: ethyl (S)(2-aminophenylpropanamido)benzoate Fmoc\ N IZ —> H2N m 0 O\/ o O\/ Compound obtained in step A was treated with 4.6 ml of DEA (6 eq.) in THF at RT for one night, in order to remove the Fmoc-protection. THF was removed and the residue was dissolved in n-hexane until formation of a solid. The solvent was removed and the product was washed twice with fresh n-hexane. 2.2 g of product was obtained.
STEP C: ethyl (S,E)(2-(3-(benzo[l ,3]dioxolyl)acrylamido) phenylpropanamido)benzoate o \ H < OH + H \ N 0 < H 0 0v O o 0V (E)(l,3-benzodioxolyl)propenoic acid (430 mg, 1 eq.) was dissolved in 5 ml of DMF, cooled to 00C and HATU (1.3 eq.) and DIPEA (1.3 eq.) were added. After 30 s 700 mg of compound ed in step B was added. The reaction mixture was brought at RT and stirred for 1h. The solution was then poured in water (50 ml) and the product was extracted with EtOAc. After acidification the solvent was evaporated and the crude product was purified on silica gel column.
STEP D: (S ,E)(2-(3 -(benzo [ l ,3 ]dioxol-5 -yl) acrylamido)-3 -phenylpropanamido)benzoic acid H H o \ N <0 \©\'r0\/_> H 0 OH O <o:©/Vkm O 600 mg of compound obtained in step C were dissolved in 30 ml of EtOH/THF 1:2. NaOH lN (3 eq.) was added to the solution and the on mixture was stirred at reflux for 4h.
Solvents were removed and the crude was dissolved in water. The solution was acidified with HCl 6N and precipitation of t was observed. The filtered solid was suspended in EtzO and ed.
STEP E: (S,E)(2-(3-(benzo[d][l,3]dioxolyl)acrylamido)phenylpropanamido)-N- hydroxybenzamide o o H H o \ NJEEN o \ < H —> N/QEN H OH < O o n O 0 \OH 0 o 400 mg of nd obtained in step D (1 eq.) were dissolved in 2.5 ml of DMF. After cooling in ice bath, HATU (1.3 eq.) and DIPEA (1.3 eq.) were added and the e was stirred for 1h. Finally a solution of NHZOH 3M in DMF (3 eq.) was added and the reaction mixture was stirred at RT for 4 h. the mixture was then poured into water and the precipitated solid was filtered and suspended in dioxane and the mixture was stirred and warmed. The final product was obtained by tion.
EXAMPLE 2 (Compound 5D) Synthesis of (S)(2-cinnamamidophenylpropanamido)-N-hydroxybenzamide ON“ N o No STEP A: Ethyl (S)(2-((((9H-fluorenyl)methoxy)carbonyl)amino) phenylpropanamido)benzoate H2N H Fmoo\ N Fmoo\N OH + O\/ — ’ H o o H \/ O O 3 g of -Phenylalanine (7.74 mmol, 1 eq.) were dissolved in 15 ml of DMF and the solution was cooled at 00C. HATU (3.84 g, 1.3 eq.) and DIPEA (1.75 ml, 1.3 eq.) were added and the reaction mixture was stirred for half an hour. Ethyl 4-aminobenzoate (1.40g, 1 eq.) was then added and the mixture was stirred at RT for 1h. The reaction was monitored by HPLC analysis. When the reaction was complete, the solution was poured in water (150ml).
The precipitated white solid was filtered and dried. 4g of pure product was obtained.
STEP B: ethyl (S)(2-aminophenylpropanamido)benzoate Fmoc\ N H IZ —> H2N O O\/ o O\/ Compound ed in step A was treated with 4.6 ml of DEA (6 eq.) in THF at RT for one night, in order to remove the Fmoc-protection. THF was removed and the residue was dissolved in n-hexane until formation of a solid. The solvent was removed and the product was washed twice with fresh n-hexane. 2.2 g of product was obtained.
STEP C: ethyl (S)(2-cinnamamidophenylpropanamido)benzoate. ©/\)k o \ H CI \ H + N _> W” O O\/ 900 mg of t obtained in step B (1 eq.) were dissolved in DCM (25 ml) and the solution was cooled at 00C. TEA was added (1 eq.). Finally a solution of cinnamoyl chloride was added. The reaction mixture was d at room temperature for 1h. After the reaction was complete, the solution was washed with water, HCl 1N and NaHCO3 5%in water. The organic phase was dried with CaClz, ed and ated. The crude was purified on silica gel column using a mixture of toluene/EtOH as mobile phase.
STEP D: (S)(2-cinnamamidophenylpropanamido)benzoic acid O o H H \ Hg; , \ N1 EN 0 O\/ 0 OH 0 o Ethyl (S)(2-cinnamamidophenylpropanamido)benzoate ed in step C (400 mg, 1 eq.) was dissolved in a mixture of OH and treated with NaOH 1N (3 eq.) for 1h.
Solvent was removed and crude was dissolved in water. Solution was acidified with conc.
HCl. A solid is formed, filtered and used for the next step.
STEP E: cinnamamidophenylpropanamido)-N-((tetrahydro-2H-pyran yl)oxy)benzamide Wfléo o H H H H a Wfl’i N 0 OH 0 \or:o o o 400 mg of compound obtained in step D (1 eq.) were dissolved in 2.5 ml of DMF. After cooling in ice bath, HATU (1.3 eq.) and DIPEA (1.3 eq.) were added and the mixture was stirred for 1h. Finally O-(tetrahydro-2H-pyran—2-yl)hydroxylamine (3 eq.) was added and the reaction e was stirred at RT for 4 h. The mixture was then poured into water and the precipitated solid was filtered and purified on silica gel column with a mixture of DCM/MeOH/NH3 25:1:0.1 as mobile phase.
STEP F: (S)(2-cinnamamidophenylpropanamido)-N-hydroxybenzamide O O H H \ N H H —> H O n O N\OU ©M \OH O 0 Compound obtained in step E (200 mg, 1 eq.) was dissolved in MeOH (50 ml) and treated with TFA (1.5 ml). The reaction mixture was stirred at RT overnight. The solvent and the ing TFA were removed by evaporation and the crude was suspended in EtzO and filtered. 120 mg of pure product were obtained.
EXAMPLE 3 (Compound SDZ) Synthesis of (S,Z)-N-hydroxy(3-phenyl(3-phenylacrylamido)propanamido)benzamide EJLO\ N o No STEP A: Ethyl (S)(2-((((9H-fluorenyl)methoxy)carbonyl)amino) phenylpropanamido)benzoate H2N H Fmoo N Fmoo OH + \ O\/ —’ H H O o\/ O O 3 g of Fmoc-L-Phenylalanine (7.74 mmol, 1 eq.) were dissolved in 15 ml of DMF and the solution was cooled at 00C. HATU (3.84 g, 1.3 eq.) and DIPEA (1.75 ml, 1.3 eq.) were added and the on mixture was stirred for half an hour. Ethyl 4-aminobenzoate (1.40g, 1 eq.) was then added and the mixture was stirred at RT for 1h. The reaction was monitored by HPLC analysis. When the reaction was complete, the solution was poured in water (150ml).
The precipitated white solid was ed and dried. 4g of pure product was obtained.
STEP B: ethyl (S)(2-aminophenylpropanamido)benzoate Fmoc\N N H —> H2” 0 O\/ o O\/ Compound obtained in step A was treated with 4.6 ml of DEA (6 eq.) in THF at RT overnight, in order to remove the Fmoc-protection. THF was removed and the e was dissolved in n-hexane until formation of a solid. The solvent was removed and the product was washed twice with fresh n-hexane. 2.2 g of t was obtained.
STEP C: ethyl (S,Z)(3-phenyl(3-phenylacrylamido)propanamido)benzoate.
O H + N \ \ H2N H OH O 0 O\/ (Z)phenylacrylic acid (1 eq.) was dissolved in 5 ml of DMF and the solution was cooled at 00C. HATU (1.3 eq.) and DIPEA (1.3 eq.) were added and the reaction mixture was stirred for one hour. Ethyl (2-aminophenylpropanamido)benzoate (1 eq.) obtained in step B was then added and the mixture was stirred at RT overnight. The reaction was monitored by HPLC is. When the reaction was complete, the on was poured in water. Crude product was extracted with EtOAc and purified on silica gel column, using a mixture of toluene/EtOAC 6:4 as mobile phase.
STEP D: (S,Z)(3-phenyl(3-phenylacrylamido)propanamido)benzoic acid \ N H n —> \ o O\/ O OH Ethyl (S,Z)(3-phenyl(3-phenylacrylamido)propanamido)benzoate obtained in step C (250 mg, 1 eq.) was dissolved in a mixture of THF/EtOH and treated with NaOH lN (3 eq.) for 24h. Solvent was removed and crude was dissolved in water. Crude product was purified by silica gel column, eluting with a mixture of Toluene/EtOAc.
STEP E: 4-((S)phenyl((Z)phenylacrylamido)propanamido)-N-((tetrahydro-2H-pyran— xy)benzamide f o o \ 1L H , 1\ 11 H N N H H o OH o H \o o O O 200 mg of compound obtained in step D (1 eq.) were dissolved in 1 ml of DMF. After cooling in ice bath, HATU (1.3 eq.) and DIPEA (1.3 eq.) were added and the mixture was stirred for 1 h. Finally O-(tetrahydro-2H-pyranyl)hydroxylamine (1 eq.) was added and the reaction mixture was stirred at RT for 4 h. The mixture was then poured into water and the precipitated solid was filtered and purified on silica gel column with a mixture of EtOAc/Toluene 7:3 as mobile phase.
STEP F: (S,Z)-N-hydroxy(3 -phenyl(3 lacrylamido)propanamido)benzamide i O H O \ N ZI N \ H —> N O N\ H H O O Compound obtained in step E (110 mg, 1 eq.) was dissolved in MeOH (25 ml) and treated with TFA (1 ml). The reaction mixture was stirred at RT for 4 hours. The solvent and the exceeding TFA were removed by evaporation and the crude was suspended in EtzO and filtered. 80 mg of pure product were obtained.
EXAMPLE 4 und 1D) Synthesis of 4-(2-(3-(benzo[1,3]dioxolyl)acrylamido)(4- methoxyphenyl)propanamido)-N-hydroxybenzamide o \meo STEP A: Ethyl (S)(2-((((9H-fluorenyl)methoxy)carbonyl)amino) phenylpropanamido)benzoate H2N H Fmoo N Fmoo OH + \ O\/ —’ H N o o H \/ O O 3 g of Fmoc-L-Phenylalanine (7.74 mmol, 1 eq.) were dissolved in 15 ml of DMF and the solution was cooled at 00C. HATU (3.84 g, 1.3 eq.) and DIPEA (1.75 ml, 1.3 eq.) were added and the reaction mixture was stirred for half an hour. Ethyl 4-aminobenzoate (1.40g, 1 eq.) was then added and the mixture was d at RT for 1h. The reaction was monitored by HPLC analysis. When the reaction was complete, the solution was poured in water (150ml).
The precipitated white solid was filtered and dried. 4g of pure product was obtained.
STEP B: ethyl (S)(2-aminophenylpropanamido)benzoate Fmoc\N N H , H2N O O\/ o O\/ Compound obtained in step A was treated with 4.6 ml of DEA (6 eq.) in THF at RT overnight, in order to remove the Fmoc-protection. THF was removed and the residue was dissolved in n-hexane until ion of a solid. The t was removed and the product was washed twice with fresh n-hexane. 2.2 g of t were obtained.
STEP C: ethyl (S,E)(2-(3-(benzo[1,3]dioxolyl)acrylamido)—3— phenylpropanamido)benzoate <0 \ + H \ H o <0 u o 0V O o O\/ (E)—3—(1,3—benzodioxol—5—yl)prop—2—enoic acid (430 mg, 1 eq.) was dissolved in 5 ml of DMF, cooled to 0°C and reacted with HATU (1.3 eq.) and DIPEA (1.3 eq.). After 30 minutes 700 mg of compound obtained in step B was added. The reaction mixture was brought at RT and stirred for 1h. The solution was then poured in water (50 ml) and the product was extracted with EtOAc. After acidification the solvent was ated and the crude product was purified on silica gel column.
STEP D: (S,E)(2—(3 -(benzo[1,3]dioxol-5 -yl)acrylamido)-3 -(4— methoxyphenyl)propanamido)—N-hydroxybenzamide O H O \ H N il <0 H 0 mov_><Oj©/\)J\m o N‘OH A solution of compound obtained in step D in MeOH was cooled in ice bath. A solution of hydroxylamine 50% in water (15 eq.) and NaOH 1N (10 eq.) were added and the reaction mixture was stirred at RT for 1h. The solution was then lised with HCl 1N. A precipitation was observed. The pure product was obtained by simple filtration.
EXAMPLE 5 (Compound 7D) sis of (S,E)(2-(3-(2,5-dimethoxyphenyl)acrylamido)—3-(4- hydroxyphenyl)propanamido)—N—hydroxybenzamide SUBSTITUTE SHEET (RULE 26) ”WM Nm0 N\ (I) O STEP A: loading of (9H-fluorenyl)methyl (4-(hydroxycarbamoyl)phenyl)carbamate on hydroxylamine Wang resin N \Fmoc /NH2 Fmoo 0 + —> The reaction was performed in an empty SPE plastic filter tube, using an Activotec PLS 4 X 6 Organic Synthesizer.
After swelling of the resin with DCM and DMF, a solution of 4-((((9H-fluoren—9- yl)methoxy)carbonyl)amino)benzoic acid (4 eq.), HATU (4 eq.), HOAt (4 eq.) and DIPEA (8 eq.) in DMF was added. The reaction e was shaken at RT overnight. The resin was then filtered and washed with DMF and DCM.
STEP B: first coupling /©/ \Boc0 N ‘ Fmoc Resin from step A (200 mg, 1 eq.) was swelled in DMF, then filtered. Fmoc-deprotection was carried out by a double treatment of the resin with a solution of piperidine 20% in DMF for 30 minutes. The solution was filtered off and the resin was washed with DMF. A solution of (S)- 9H-fluorenyl)methoxy)carbonyl)amino)(4-((tert— butoxycarbonyl)oxy)phenyl)propanoic acid (4 eq.), HATU (4 eq.), HOAt (4 eq.) and DIPEA (8 eq.) in DMF was added to the resin. The on mixture was shaken at RT overnight. The resin was filtered and washed with DMF and DCM.
STEP C: second coupling Boo/0O YET Wm” *Fmon H O/N O Resin from step B (1 eq.) was swelled in DMF, then filtered. eprotection was performed by a double treatment of the resin with a solution of piperidine 20% in DMF for 30 minutes. The solution was d off and the resin was washed with DMF. A solution (E)—3— (2,5—dimethoxyphenyl)acrylic acid (4 eq.), PLATU (4 eq.), HOAt (4 eq.) and DIPEA (8 eq.) in DMF was added to the resin. The reaction mixture was shaken at RT overnight. The resin was filtered and washed with DMF and DCM.
STEP D: cleavage Cleavage of the product from the resin was obtained by treatment with TFA 50% in DCM at RT for 30 minutes. During this step the Boo—protection was also removed.
Crude was purified on SPE cartridge.
EXAMPLE 6 The following compounds were ed using the procedure described in example 5: SUBSTITUTE SHEET (RULE 26) 1 \ 21 \ H H H CED/TH” I H OH N\OH Oorrpound 2D Oorrpound 27D <3 0 ' H H 2 \ 22 \ I mmn‘m. H H N\OH Oorrpound 8D Oorrpound 28D H H 3 23 \ H “GYMH < H / o oo ndQD o oo nd293 o/ o H O \ H 4 24 \ H ”N\ H H \ N\OH / und 10D Oorrpound 30:) CO H O I/ H O \ 25 H H l \ O N\OH < HN\ I 0” Compound 11D Oon'pound 31D H k /0 \ 6 H ”N\ 26 \ H T OH < H H N\OH o und12D o oo nd32D ACCTH H H H 7 27 \ O N\OH < H H I O Oorrpound 13D Oorrpound 33D 0/ 0 \II 0 8 28 W H H H N\OH N0“ 0 nnd14D o oo nd34D < H H N\OH Oorrpound 35D 30 :DM H < H Nag/KW Corrpound 36D SUBSTITUTE SHEET (RULE 26) O CI H H H2 N\OH KOH Corrpound 17D nd 37D . O\ 12 /“*©f*mr 32 WW? H H H H N\OH N\OH c on nd18D 0 cc nd38D SUBSTITUTE SHEET (RULE 26) EXAMPLE 7 Cytotoxicity activity in vitro The compounds of the present invention are small molecules of formula (1), terised by the presence of a metal chelating moiety, the benzohydroxamate, an oc-aminoacidic N- acylated central core and an (x,B-unsaturation at the acyl-moiety. This particular structural feature seems responsible for the high inhibitory activity on cancer stem cell lines and on HCT116 cells (ATCC CCL-247), a human colorectal carcinoma cell line widely used in cancer biology both in vitro and in vivo (Botchkina Cancer Genom m 6, 19-30, 2009; Yeung PNAS 107, 3722-3727, 2010).
The cytotoxicity activity was ted as follows: - Pre—B ia cell line 697 was seeded at 2 )(105 c/well; - Colon carcinoma cell lines HCT116, HT29 and COLO2015 were seeded at 4 X103, 4 :103 and 10 5:103 c/well respectively; - Primary human kidney cells were seeded at 1,5 5:103 and 6,5 )(103 in two separate ments; - Human PBMC were seeded at 5xlO5 c/well; - Colon cancer stem cells (CSC) were seeded at 3 X103; Testing compounds were added after 24 h and incubated for 72 h (48 h for 697 cell line). The concentrations of molecules ranged from 10000 nM to 1.5 nM (10000 to 1 nM for 697 cell line). The cytotoxic activity of nds was evaluated using the CellTiter 96® Aqueous One Solution Cell Proliferation Assay (Promega) which measures the functionality of mitochondria ing to manufacturer’s instructions. For Colon cancer stem cells viability was determined with CellTiter-Glo Luminescent cell viability assay (Promega).
In the y cytotoxicity screening performed on 697 cell line the activity of compounds D was similar or higher than that of saturated compounds.
WO 15292 The proliferation inhibitory activity of the unsaturated compounds of the present invention on HCT116 cells is 30 to 80 fold higher than the one ted by saturated analogues of the prior art US7635788, while it is at least similar or 3.5 to 40 fold higher on the stem cells (see table 1). In particular, all the compounds of the invention are resulted more active than the saturated analogues of the prior art on HCT116 cells.
The compound cytotoxicity was confirmed by further assays on two other colon cancer cell lines HT29 (ATCC HTB38) and C010205 (ATCC CCL222) and on colon cancer stem cells (CSCs) (see table 2 and 3). It is worth noting that cytotoxic activity toward human primary kidney cells and peripheral blood mononuclear cells (PBMC) isolated from healthy donors was lower compared to tumor cells cytotoxicity (see table 4).
The cis-form prepared according to Example 3 is resulted less potent of the form on HCT116 cells.
Furthermore the cis- form is ally less stable than the trans. In a force degradation test all the compounds in the trans-form show high stability even at high temperatures (15 days at 800C) and at low pH (15 days at pH 2), while cis-analogue was little less stable at 800C and tely less stable at pH 2.
Table 1: Comparison results on colon cancer cell lines OOVPCXJD I-CT116 n T 0801 T Tox697 n T OOVPCXJD I-DT116 n T 0801 n T Tox697 24 I. I 18 554 I. l127 702 n. l203 18 I. 85 76 I. l105 O c ” 316 160 72 90 431 136 53 770 13 I. 45 194 l 59 Table 2: Cytotoxic activity of compounds on colon cancer cell lines HCT-116 HT29 COL0205 —IC 50 nM IC 50 nM 1c 50 nM —*——157 2D 61 1436 na 2 not available The ty of compounds at any dose is calculated as percentage of tion versus vehicle-treated control (= 0). The IC50s are extrapolated from the inhibition dose/response curve using GraphPad Prism program.
SUBSTITUTE SHEET (RULE 26) 2017/067850 Table 3: Cytotoxic activity of compounds on colon cancer stem cell Compound CSCl CSC2 CSC3 IC 50 (nM) IC 50 (nM) IC 50 (nM) 5D 26 102 230 4D 13 102 91 1D 19 114 85 2D 50 100 70 Table 4: Cytotoxic activity of compounds on primary renal cells and PBMC Compound PBMC 1 PBMC 2 Renal.epl Renal.ep2 IC 50 (nM) IC 50 (nM) IC 50 (nM) IC 50 (nM) SD 147 595 1061 511 4D 359 601 778 na 1D 80 152 462 321 2D 89 42 na na Antitumor activity in vivo The compounds of this invention also showed ty in vivo in a xenograft model of colon cancer where the human HTC116 ctal carcinoma cell line was injected subcutaneously (sc) in CD1 nude female mice (see table 5).
Female CD-1 nude mice, 5 weeks of age and 20 to 22 g of weight, were housed in the animal house of the Italfarmaco Research Centre. Mice were maintained in micro isolator cages and supplied with sterile food and water under standard conditions.
Xenografts were generated by sc injection of 7><106 HCT116 cells in the right flank of the animals.
Tumor sizes were ined by caliper measurements and tumor volumes were calculated according to the following formula: Tumor volume (m3) = (w2 x 1)/2 where “w” is the width and “l” is the length of the carcinoma in mm.
Tumor growth was followed by volume measurement three times a week.
WO 15292 Tumor mass d a measurable size three weeks after transplantation, at this time, treatments began.
Two reference compounds were also used in the experiment. The cinnamic hydroxamic acid nostat and 5-FU. The compounds were administered at the MTD according to previous experiments or as described in the literature. As shown in table 5, the compounds of this invention were able to reduce tumor size and volume, their activity being comparable to that of the two reference compounds.
Of note, the treatment with the reference compound 5-FU lead to a remarkable leukopenia (70.4%) at the end of the treatment while compound 4D showed a able iveness (54% reduction of tumor compared to 61% obtained with 5-FU) but a much less pronounced leukopenia (23.7%).
Furthermore, no thrombocytopenia and no weight loss were observed at the end of the treatment indicating that at the effective doses, the compounds object of this invention exhibited a favourable therapeutic window in this animal model.
The nds of the present invention show good in vitro metabolic stability, both when incubated with human ction and in human plasma. They also show a good pharmacokinetic profile in preliminary studies in preclinical species.
Table 5: y of the anti-tumor in vivo activity % inhib % 111th WBC vs % of.Body % inhib PLT tumor vs CTRL T0 Weight vs CTRL T0 Dose, route of CTRL after 44 variation. .
Compound after 44 days admlnlstratlon. . . after 44 days of lntragroup. of treatment days of treatment After 65 days (vs predose) treatment (vs of treatment predose) 876:). -4.2 Panobinostat 1 mg/kg, ip 34 -l.08 (20.3) 3 1.8 -6.4' 5 mg/kg’ 1p 6 3 (45.7) (22.9) 23.7 , -10.3; 4D 5 mg/kg, 1p 54 -0.97 029.4) (27.3) .2 , -6.3, 4D 10 mg/kg, 1p 39 1.24 ) (22.7) -11.7 -14.3' 1D 0 5 “lg/kg ' ’ . 2 , 1p 3 0.41 (-89.4) (31.9) 16.2' -8.3' 1D 1 mg/kg’ 1p' 22 ’ ’ 1.42 (42.1) (—25) . 1.6; 2.8; 2D 1 mg/kg, 1p 33 -1.84 (-67) (42.2) 26' 1.9' -FU 10mg/kg, 1p' 36 ’ ’ - 3 .22 025.5) (43.2) 70 mg/kg, ip, once 70.4; -12.4; -FU 61 1.57 a week (49.7) (-29.8) Vehicle, CTRL na (69.6) (-15.4) 6.7 200pL/mice

Claims (12)

Claims
1. A compound of the a (I) and pharmaceutically acceptable salts and stereoisomers thereof, wherein: n is 0, 1 or 2; A is H, provided that when A is H, R1 and R2 are absent, or is a mono or di-carbocyclic residue, ally partially or totally unsaturated, comprising carbon atoms and optionally one or more heteroatoms selected from N, S or O; R1 and R2 are independently selected from the group consisting of –H, –OH, -OMe, -CN, - NH2, -NO2, -Cl, -COOH, -halogen, -CF3, -N(Ra)2, linear or branched C1-C4 alkyl, aryl, arylalkyl, rbonyl, alkoxy, aryloxy residue, sulphonylamino and -CH2N(CH2CH3)2; Ra is a linear or branched C1-C3 alkyl residue; X is C or N; R3 and R4 are independently selected from the group consisting of –H, -OMe, -OPh, -NO2, - NMe2, -NH2, -halogen, -CF3, 2, linear or branched C1-C4 alkyl, aryl, arylalkyl, arylcarbonyl, , aryloxy residue and sulphonylamino, or R3 and R4 together form a heteropentacyclic moiety comprising (-OCH2O-).
2. The compound according to claim 1, in which: n is 0, 1 or 2; A is H, provided that when A is H, R1 and R2 are absent, or is a mono or di-carbocyclic residue, optionally partially or totally unsaturated, comprising carbon atoms and optionally one or more heteroatoms selected from N, S or O; R1 and R2 are independently selected from the group ting of –H, –OH, -OMe, -CN, - NH2, -NO2, -Cl, -COOH and -CH2N(CH2CH3)2; Ra is a linear or branched C1-C3 alkyl residue; X is C or N; R3 and R4 are independently ed from the group consisting of –H, -OMe, -OPh, -NO2, - NMe2 and -NH2, or R3 and R4 together form a heteropentacyclic moiety sing (- OCH2O-).
3. The compound according to claim 1 or 2, in which n is 1, X is C; R1 and R2 are independently –H, -Cl or –OMe; R3 and R4 are independently –H, -NMe2, or R3 and R4 together form a heteropentacyclic moiety comprising (-OCH2O-).
4. The compound according to claim 1, wherein the compound is selected from among: - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](4-methoxyphenyl)propanamide (1D); - -[4-(hydroxycarbamoyl)phenyl](4-methoxyphenyl)[[(E)phenylprop enoyl]amino]propanamide (2D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-propanamide (4D); - (2S)-N-[4-(hydroxycarbamoyl)phenyl]phenyl[[(E)phenylprop enoyl]amino]propanamide (5D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](4-hydroxyphenyl)propanamide (7D); - (E)(2,5-dimethoxyphenyl)-N-[(1R)[4-(hydroxycarbamoyl)anilino]indanyl- 2-oxo-ethyl]propenamide (8D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-butanamide (9D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-propanamide (10D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](2-naphthyl)propanamide (11D); - (2,5-dimethoxyphenyl)-N-[2-[4-(hydroxycarbamoyl)anilino]oxo-ethyl]prop- 2-enamide (12D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- xycarbamoyl)phenyl]propanamide (13D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](3-quinolyl)propanamide (14D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](3-pyridyl)propanamide (15D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](4-nitrophenyl)propanamide (16D); - (2S)[[(E)(4-aminophenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-propanamide (17D); - (2S)(4-aminophenyl)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]propanamide (18D); - (2S)(4-cyanophenyl)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]propanamide (19D); - -[4-(hydroxycarbamoyl)phenyl][[(E)(4-nitrophenyl)propenoyl]amino]- 3-phenyl-propanamide (20D); - (2S)[[(E)(2,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](1H-imidazolyl)propanamide (21D); - (2S)[[(E)(3,4-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](3-pyridyl)propanamide (22D); - (2S)[[(E)(3,5-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](3-pyridyl)propanamide (23D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](3-pyridyl)propanamide (24D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino](benzothiophenyl)-N- [4-(hydroxycarbamoyl)phenyl]propanamide (25D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]thiazolyl-propanamide (26D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-butanamide (27D); - (2S)[[(E)(3,4-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]phenyl-propanamide (28D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino](4-cyanophenyl)-N-[4- (hydroxycarbamoyl)phenyl]propanamide (29D); - -[[(E)(3,4-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](1H-indolyl)propanamide (30D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](1H-indolyl)propanamide (31D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](4-nitrophenyl)propanamide (32D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino](4-chlorophenyl)-N-[4- (hydroxycarbamoyl)phenyl]propanamide (33D); - (2S)(4-aminophenyl)[[(E)(3,4-dimethoxyphenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]propanamide (34D); - (2S)(4-aminophenyl)[[(E)(1,3-benzodioxolyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl]propanamide (35D); - 4-[(2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino][4- (hydroxycarbamoyl)anilino]oxo-propyl]benzoic acid (36D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino](3,4-dichlorophenyl)-N- [4-(hydroxycarbamoyl)phenyl]propanamide (37D); - -[4-(hydroxycarbamoyl)phenyl](4-methoxyphenyl)[[(E)phenylprop enoyl]amino]propanamide (38D); - (2S)[[(E)(4-dimethylaminophenyl)propenoyl]amino]-N-[4- xycarbamoyl)phenyl]phenyl-propanamide (39D); - (2S)[[(E)(4-dimethylaminophenyl)propenoyl]amino]-N-[4- (hydroxycarbamoyl)phenyl](4-methoxyphenyl)propanamide; 2,2,2-trifluoroacetic acid (40D); - (E)-N-[2-[[4-(hydroxycarbamoyl)phenyl]methylamino]oxo-ethyl]phenyl-prop enamide (41D); - (E)(1,3-benzodioxolyl)-N-[2-[[4-(hydroxycarbamoyl)phenyl]methylamino] oxo-ethyl]propenamide (43D); - (2S)[4-(diethylaminomethyl)phenyl]-N-[4-(hydroxycarbamoyl)phenyl][[(E) phenylpropenoyl]amino]propanamide; 2,2,2-trifluoroacetic acid (45D); - (2S)[[(E)(1,3-benzodioxolyl)propenoyl]amino][4- (diethylaminomethyl)phenyl]-N-[4-(hydroxycarbamoyl)phenyl]propanamide; 2,2,2- trifluoroacetic acid (46D); - (2S)-N-[4-(hydroxycarbamoyl)phenyl](2-naphthyl)[[(E)(6-phenoxy pyridyl)propenoyl]amino]propanamide (47D).
5. The compound according to any one of claims 1 to 4, in which the stereoisomeric form is the trans-form.
6. Use of the compound according to any one of claims 1 to 5, in the manufacture of a medicament for the prevention and/or treatment of cancer.
7. Use of the compound according to any one of claims 1 to 5, in the manufacture of a medicament for the prevention and/or treatment of colorectal , lung, brain, prostate or logical cancers or hematologic malignancies.
8. Use of the compound according to any one of claims 1 to 5, in the manufacture of a medicament for the prevention and/or treatment of metastatic, recurrent and drug-resistant diseases.
9. A pharmaceutical ition comprising a therapeutically effective quantity of at least one of the nds of the formula (I) or pharmaceutically acceptable salts and stereoisomers thereof according to any one of claims 1 to 5 together with at least one pharmaceutically acceptable excipient.
10. The pharmaceutical composition according to claim 9, le to be administered by enteral route, parenteral route, oral route, topical route, or inhalatory route.
11. The pharmaceutical composition according to claim 9 or 10, in the form of a liquid or a solid, preferably in the form of capsules, tablets, coated s, powders, granules, creams or ointments.
12. The compound according to claim 1, substantially as herein described with reference to any one of the Examples thereof.
NZ749473A 2016-07-18 2017-07-14 Benzo-n-hydroxy amide compounds having antitumor activity NZ749473B2 (en)

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