NZ780173B2 - Techniques for predicting, detecting and reducing aspecific protein interference in assays involving immunoglobulin single variable domains - Google Patents
Techniques for predicting, detecting and reducing aspecific protein interference in assays involving immunoglobulin single variable domainsInfo
- Publication number
- NZ780173B2 NZ780173B2 NZ780173A NZ78017312A NZ780173B2 NZ 780173 B2 NZ780173 B2 NZ 780173B2 NZ 780173 A NZ780173 A NZ 780173A NZ 78017312 A NZ78017312 A NZ 78017312A NZ 780173 B2 NZ780173 B2 NZ 780173B2
- Authority
- NZ
- New Zealand
- Prior art keywords
- isv
- multiparatopic
- biparatopic
- multispecific
- bispecific
- Prior art date
Links
Abstract
Provided are bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic immunoglobulin single variable domain (ISV) constructs comprising an ISV at their C-terminal end, wherein said ISV has the sequence VTVSS(X)n at its C-terminus, wherein n = 1 to 10 and X represents alanine (A), glycine (G), valine (V), leucine (L) or isoleucine (I).
Claims (9)
1. Bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct with an ISV at its C-terminal end, wherein said ISV construct has the sequence VTVSS(X)n at its C-terminus, wherein n = 1 to 10 and each X is an amino acid residue that is independently chosen from alanine (A), glycine (G), valine (V), leucine (L) or isoleucine
2. Bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct according to claim 1, wherein the ISV at the C-terminal end has been modified by introducing one or more amino acid substitutions, additions or deletions in the C- terminal region comprising the C-terminal sequence VTVSS (SEQ ID NO:33) and the amino acid residues at positions 11, 13, 14, 15, 82, 82a, 82b, 83, 84, 107 and 108, wherein the amino acid residues are numbered according to Kabat.
3. Bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct according to claim 2, wherein the ISV at the C-terminal end has been modified by introducing one or more amino acid substitutions, additions in the C-terminal region.
4. Bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct according to any of claims 1 to 3, that has a substantially reduced tendency (such as at least a statistically relevant reduced tendency) to give rise to protein interference, compared to the same ISV construct but without the modifications.
5. Bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct according to any of claims 1 to 4, that has, in the assay described in Example 3, substantially reduced ability to be bound by the polyclonal antibody described in Example 2, compared to the same ISV construct but without the modifications.
6. Bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct according to any of claims 1 to 5, that has, in the assay described in Example 5, substantially reduced ability to be bound by the polyclonal antibody described in Example 2, compared to the same ISV construct but without the modifications.
7. Bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct according to any of claims 1 to 6, wherein each ISV is a humanized VHH or a camelized VH, such as a camelized human VH.
8. Bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct according to any of claims 1 to 7, that comprise a serum albumin-binding peptide or binding domain.
9. Pharmaceutical composition that comprises a bivalent, multivalent, bispecific, multispecific, biparatopic or multiparatopic ISV construct according to any of claims 1 to 8 and at least one suitable carrier, diluent or excipient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NZ809551A NZ809551A (en) | 2011-06-23 | 2012-06-25 | Techniques for predicting, detecting and reducing aspecific protein interference in assays involving immunoglobulin single variable domains |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NZ780173A NZ780173A (en) | 2025-01-31 |
| NZ780173B2 true NZ780173B2 (en) | 2025-05-01 |
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