WO2014094375A1 - Bee venom composition with acne removing effect - Google Patents

Abstract

A bee venom composition with an acne removing effect comprises a cosmetic matrix component and bee venom. The bee venom is prepared by using the following method: taking out raw bee venom, dissolving the raw bee venom by using water, performing ultra filtration by using an ultra filtration membrane with the molecular weight cutoff 10000Da, and freezing and drying the filter liquor, so as to obtain the bee venom.

Description

 Bee venom composition with acne effect

Technical field:

 The invention belongs to the field of cosmetics, and particularly relates to a bee venom composition having acne effect. Background technique:

 With the accelerated pace of life, increased pressure on work and life, and environmental pollution and food safety issues, the incidence of acne has increased year by year. Acne is a common chronic hair follicle sebaceous gland inflammatory disease, which occurs in areas with strong sebum secretion such as the face, chest and upper back. It has a long course of disease and is prone to recurrence. It is often manifested as whitehead acne, blackhead acne or inflammatory papules. Serious pustules, nodules, cysts or scars. If the inflammation is obvious, it will cause pain or pain, accompanied by seborrhea, and even orange peel. The incidence of acne in young men and women is extremely high. It not only seriously affects the beauty of the face, but also affects the social and psychological aspects of the person, causing negative emotions such as distrust, embarrassment, loss, anxiety, nervousness, irritability and depression. Thereby affecting the quality of life.

 At present, the cosmetics for treating acne on the market are often added with chemical fungicides, hormones, astringents and anti-inflammatory agents. Although they can be sterilized or anti-inflammatory in a short time, they are irritating to the skin, and long-term use will increase the burden on the skin. And cause allergic symptoms such as redness, suppuration or pain in the skin, and because the pathogen is highly susceptible to drug resistance, the acne will soon recur and form scars or pigmentation. Antibiotics and hormonal substances have been banned by the Cosmetic Hygiene Code (2007 Edition) due to long-term side effects. In addition, traditional Chinese medicine acne cosmetics, due to slow onset, long treatment period, and many unpleasant smells and colors, will leave a black or red mark after healing, and are not welcomed by consumers. Therefore, it is particularly urgent to develop a safer, more durable and comprehensive acne cosmetic. Summary of the invention:

 The object of the present invention is to provide a safer and lasting acne-producing effect against the problems of irritating acne cosmetics, skin allergies, and easy to leave dark marks and smells in Chinese medicine acne cosmetics. The bee venom composition, the bee venom composition has clear efficacy ingredients, stable quality, no toxic and side effects, does not cause skin allergy, does not leave scars or pigmentation after acne cure, and is not easy to relapse after being stopped.

The bee venom composition having acne effect of the present invention comprises a acne cosmetic matrix component characterized by further containing bee venom. Preferably, the bee venom is prepared by the following method, and the crude bee venom is dissolved in water, and then ultrafiltered through an ultrafiltration membrane with a molecular weight cut off of 1000OOD, and the filtrate is freeze-dried to obtain bee venom.

The bee venom purification method simplifies the bee venom purification process, saves the cost, and can effectively enrich and concentrate the active substances such as melittin and bee venom peptide which have significant cosmetic skin care effects, and remove hyaluronic acid. Enzymes, phospholipase A ₂ and other substances that cause skin allergies, inflammation and pain, while ensuring safety, no skin irritation and allergies, significantly enhance the efficacy of acne. The purification method solves the problems that the active substance having the beauty and skin care effect in the bee venom has not been effectively enriched in the prior art, fills the blank application of the bee venom in the acne cosmetics, and fully exerts its acne effect.

 Preferably, the bee venom is present in an amount of 0.00001% to 2% of the total mass of the bee venom composition.

 The bee venom is preferably prepared by the following method: taking crude bee venom and adding 10-30 times volume of water to fully dissolve, centrifuging 5000-1000 g for 10-30 min, and the supernatant is ultrafiltered through an ultrafiltration membrane with a molecular weight cut off of 100OODa. Thereafter, the filtrate was freeze-dried to obtain bee venom.

 In order to enhance anti-inflammatory, sunscreen, moisturizing, anti-acne and other effects, the bee venom composition having acne effect of the present invention preferably further contains seaweed polysaccharide and pearl powder, and the content of seaweed polysaccharide accounts for 0.01% of the total mass of the bee venom composition. 5%, the content of pearl powder accounts for 0.2%-15% of the total mass of the bee venom composition.

 The seaweed polysaccharide refers to sodium alginate, laver polysaccharide, wakame polysaccharide, pearl vegetable polysaccharide, broccoli polysaccharide, Eucheuma polysaccharide, Sargassum polysaccharide, kelp polysaccharide, Artemisia polysaccharide, Spirulina polysaccharide, Sargassum polysaccharide, A combination of one or more of a copper algae polysaccharide, a Fucus polysaccharide, a carrageenan polysaccharide, a big leaf algae polysaccharide, and a chlorella polysaccharide. It is commercially available and can also be prepared according to existing methods.

 The pearl powder of the present invention refers to a combination of one or more of nano pearl powder, water soluble pearl powder, and pearl hydrolyzate liposome. It is commercially available and can also be prepared according to existing methods.

 In order to enhance the effect of clearing pores, balancing oil secretion and the like, the bee venom composition having acne effect of the present invention may preferably contain PCA zinc and octanoylsalicylic acid, and the content of PCA zinc accounts for 0.01% of the total mass of the bee venom composition. %-1.5%, the content of octanoylsalicylic acid is from 0.1% to 2% of the total mass of the bee venom composition.

 The PCA zinc, octanoylsalicylic acid and cosmetic bases of the present invention are all commercially available.

 The crude bee venom may be crude bee venom and/or bee crude bee venom, which may be collected by a bee venom collector or purchased from the market.

The venom-promoting bee venom composition of the present invention is prepared by the following method, and the bee venom is prepared in powder form or pre-formed into a 10%-50% aqueous solution, and is homogenized or mixed with other ingredients. It can be formulated according to the conventional process according to the type of acne cosmetics formulated, and can be made into acne cosmetics such as cream, lotion, liquid, gel, gel or mask.

 The bee venom composition having the acne effect of the invention has good stability, uniform coating and good spreadability. The experimental results of animal experiments and human trials prove that the bee venom composition with acne effect of the invention is safe, non-irritating or photo-sensitizing to the skin, can effectively prevent and reduce the damage of ultraviolet rays to the skin, and can also sterilize and reduce inflammation. Moisturizes, balances oil secretion, and improves skin's self-protection.

 The bee venom composition having the acne effect of the present invention can be used as a general cosmetic or a functional cosmetic having an acne action.

 The solution of the present invention is based on the inventors' understanding of the conditions, pathogenesis, prevention and treatment methods of acne, and the beauty and skin care effects of functional active substances, combined with the research achievements of modern acne cosmetics and pharmacology, from among many functional active substances. Screening for bee venom, seaweed polysaccharide, pearl powder, PCA with significant acceleration of keratinocyte metabolism, dredging hair follicles, regulating oil secretion, bacteriostatic, bactericidal, anti-inflammatory, moisturizing, anti-acne and scar, anti-oxidation, anti-ultraviolet damage, etc. Zinc and octanoyl salicylic acid, through scientifically organized, make them play their own synergies:

 The bee venom in the bee venom composition having the acne effect of the invention is rich in active components such as melittin, bee venom peptide, catecholamine, free amino acid, vitamin, trace element and glycerin, and has small molecular weight and low immunogenicity. Not susceptible to allergic reactions.

 Melittin can effectively inhibit the proliferation of bacteria and fungi such as Propionibacterium acnes, Escherichia coli, Pseudomonas aeruginosa, Streptococcus and Staphylococcus aureus, reduce the skin's inflammatory response to acne, and has an analgesic effect. It also has significant anti-oxidation, anti-ultraviolet radiation and other effects. The bee venom peptide can improve the microcirculation, promote the synthesis of elastic fibers and collagen in the dermis, restore skin tension and elasticity, and promote the rapid closure of acne, repair existing sputum or prevent the formation of dark sputum. Catecholamine has an anti-inflammatory effect on damaged skin caused by acne, light, etc., and can reduce the formation of acne scars. Active substances such as free amino acids, vitamins and trace elements can provide nutrition to the skin, effectively prevent UV rays from causing damage to acne and form pigmentation, improve keratin metabolism, prevent hyperkeratosis of hair follicles, enhance epidermal barrier function, and reduce water loss. Reduce air pollution to skin, regulate skin permeability, have antioxidant and promote skin self-repair. Glycerin maintains moisture in the skin, reduces sebaceous gland secretion, and promotes the penetration of other active ingredients into the deeper layers of the skin, helping to improve the skin's ability to repair itself.

The seaweed polysaccharide in the bee venom composition having the acne effect of the invention has strong moisturizing property and can soften It exfoliates and acts as a broad-spectrum bactericidal effect by dissolving the bacterial inner membrane. It also scavenges free radicals, prevents skin lipid peroxidation, resists UV-induced skin damage, and repairs damaged cells and reduces pigmentation. Seaweed polysaccharide can also accelerate the transdermal absorption of acne active ingredients, thereby improving the acne effect.

 The pearl powder in the bee venom composition having the acne effect of the invention contains amino acids such as glycine, methionine and the like which promote the regeneration of skin collagen and trace elements such as Zn, which are easily absorbed by the human body and have bactericidal and anti-inflammatory effects. Detoxification, remove acne marks and other effects. Pearl powder can also promote epidermal cell regeneration and wound healing, so that impurities such as dust, oil stains, bacteria, peeling and dead cells in the pores of the skin can be smoothly discharged, making the skin smooth, white and moist, and effective against bee venom. Has a significant synergy.

 The PCA zinc in the bee venom composition having the acne effect of the present invention replaces the sodium ion thereof with a zinc ion having a bacteriostatic action, and can effectively inhibit P. acnes, Escherichia coli, Pseudomonas aeruginosa, Streptococcus and gold. The proliferation of bacteria and fungi such as Staphylococcus aureus, and excellent moisturizing effect. Zinc can regulate the sebaceous gland secretion and prevent hyperkeratosis of the hair follicle by inhibiting 5-ct reductase, thereby maintaining the oil-water balance of the skin. DNA synthesis, protein synthesis, cell division, and the maintenance of multiple enzyme activities in human tissues are inseparable from Zn, so supplementing the skin with Zn helps maintain normal skin metabolism, promotes epidermal cell growth and wound healing, and reduces Skin allergies.

 The octanoylsalicylic acid in the bee venom composition having the acne effect of the invention has a fat-soluble property and a large molecular weight, and can penetrate into the stratum corneum and the deep pores through the sebaceous glands which secrete oil by way of fusion with the lipid. Dissolve the angle plug, break down the keratin and dead skin that block the sebaceous glands and pores, improve the pore blockage, block the formation of acne, reduce or inhibit the symptoms of inflammation, remove the blackheads in the pores, and reduce the enlarged pores. It locks the action in the stratum corneum without affecting the active epidermal cells and dermal tissue. It has good stability, low irritation to the skin, and a low cumulative rate of irritation and inflammation. Salicylic acid also stimulates cell renewal and wound healing to improve skin tone, eliminate acne marks, and rejuvenate the skin.

 Compared with the prior art, the present invention has the following beneficial effects:

1. The bee venom composition having the acne effect of the present invention is selected from the pure natural bee venom as the main raw material for the specific physiological structure of the skin and its sensitivity to the ingredients used in cosmetics. Although the current addition of bee venom cosmetics is very rare, bee venom has long been included in the "International Cosmetic Raw Materials Standard Chinese Name List (2010 Edition)", which shows that the application of bee venom to cosmetics is safe and feasible. In addition, in the bee venom composition having the acne effect of the present invention, the bee venom used is purified, and the substance which is easy to cause skin allergy, inflammation and pain in the crude bee venom is effectively removed, and the bee venom peptide is used. , bee venom peptide and other ingredients Highly enriched to give a more pronounced acne effect. At the same time, the bee venom composition having the acne effect of the present invention can be quickly absorbed by the skin, does not cause skin irritation, and does not impose any burden on the skin.

 2. The bee venom composition having the acne effect of the present invention secretes oil secretion, dissolves angle plugs, inhibits bacterial proliferation, repairs scars, resists ultraviolet rays, anti-inflammatory, anti-allergic, anti-oxidation, moisturizing, anti-inflammatory pigmentation, and promotes phlegm. The acne component is absorbed through the skin and promotes the healing of acne wounds. It exerts a comprehensive and significant acne effect, which restores the skin to normal, smooth and smooth. After discontinuation, symptoms such as acne are not easy to relapse, and acne is effective, long-lasting, and safe.

 3. The bee venom composition with acne effect of the invention adopts pure natural bee venom, has good compatibility with skin, can be quickly absorbed by the skin, is safe and mild, does not cause irritation, allergic reaction, and has no skin. burden. The bee venom composition with acne effect of the invention can synergistically synergize with bee venom, seaweed polysaccharide, pearl powder, and bee venom with PCA zinc and octanoyl salicylic acid to simultaneously clear pores, balance oil secretion, and sterilize , anti-inflammatory, anti-acne marks, moisturizing, sunscreen and other effects, to comprehensively repair and improve the barrier function of the skin, completely eliminate acne. When bee venom is compounded with seaweed polysaccharide and pearl powder, the bee venom composition having the acne effect of the present invention is particularly effective for the healing of inflammatory lesions, pustules and cystic acne. When bee venom is compounded with PCA zinc or octanoylsalicylic acid, the bee venom composition having the acne effect of the present invention is particularly effective for the healing of blackhead acne, whitehead acne, punctiform, papular and atrophic acne.

 4. The bee venom composition having the acne effect of the invention has various varieties and can meet the needs of different genders, ages and skin types of people suffering from problems such as acne, adult acne, acne marks or scars. detailed description:

 The invention is further illustrated by the following examples, but the embodiments of the invention are not limited to the following examples, and equivalent variations or modifications of the method according to the invention are considered to be within the scope of the invention.

 The materials used below are commercially available unless otherwise specified.

 Example 1

 The bee venom collector was used to collect the crude bee venom of the wasp, dissolved in 25 times volume of water, centrifuged at 8000 g for 15 min, and the supernatant was ultrafiltered through an ultrafiltration membrane with a molecular weight cut off of 100 °D, and the filtrate was freeze-dried to obtain bee venom.

The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%: Phase A: carbomer 0.1%, NaC 10.8%, 1,3-butanediol 5%, propylene glycol 4%, EDTA-2Na 0.1%, deionized water balance.

Phase B: 3% of fatty alcohol polyoxyethylene ether, 9% of C ₁₂ -C ₁₅ alkyl benzoate, 2% dioctyl carbonate, 0.8% microcrystalline wax, azone/PEG-40 hydrogenated castor oil 0.2% , Hansheng gum 0.5%.

 Phase C: Bee venom 0.5% (prepared with deionized water in the form of a 50% bee venom solution).

 Phase D: 0.5% parabens and 0.2% green tea flavor.

 Process: Heat phase A and phase B to 80 ° C, respectively, and slowly add phase B to phase A while stirring. Stir and cool to 55 °C, add phase C to the mixture of phase A and phase B, stir and mix for 10 minutes, then force into phase D, mix and homogenize to obtain the bee venom composition with acne effect of this example. A acne lotion containing bee venom.

 The emulsion prepared in the embodiment has a fine hand feeling, uniform coating, no separation of oil and water, and easy absorption. Example 2

 Collect bee crude bee venom with bee venom collector, add 10 times volume of water to fully dissolve, 5000g centrifuge

After 30 min, the supernatant was ultrafiltered through an ultrafiltration membrane with a molecular weight cut off of 100 °D, and the filtrate was freeze-dried to obtain bee venom.

 The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%:

 Phase A: Carbomer 0.2%, glycerin 4%, propylene glycol 3%, deionized water balance.

 Phase B: cetearyl ether-21 2%, jojoba oil 3%, cetearyl alcohol 2%, glyceryl monostearate 1%, caprylic/capric triglyceride 4%, palmitic acid Ethylhexyl ester 3%, polydimethylsiloxane 2%, isopropyl myristate 3%, hydroxyphenylethyl ester 0.1%.

 Phase C: Bee venom 0.00001% (added as a powder).

 Phase D: Azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.2%, phenoxyethanol/hydroxybenzyl ester/hydroxyphenylbutyl ester/hydroxyphenylethyl ester/hydroxypropylpropyl ester/hydroxyphenyl isobutyl ester 0.1 %.

 Process: Heat phase A and phase B to 80 ° C, respectively, and add phase B to phase A with stirring. Stir and cool to 45 °C, add phase C to the mixture of phase A and phase B, stir and mix for lOmin, then add

Phase D, mixed and homogenized to prepare the bee venom composition having the acne effect of the present embodiment, a acne cream containing bee venom Frost.

 The cream prepared in the embodiment has a luster, a fine texture, a uniform distribution, and no separation of the oil and water phases. Example 3

 Purchasing wasp crude bee venom from Wuhan Yimin Bee Products Co., Ltd., adding 30 times volume of water to fully dissolve, lOOOOg centrifugation for 10 min, the supernatant was ultrafiltered through ultrafiltration membrane with molecular weight cut off of 100OOD, and the filtrate was freeze-dried to obtain wasp. poison.

 Bee bee venom was collected by bee venom collector, dissolved in 30 times volume of water, centrifuged lOOmin for lOmin, and the supernatant was ultrafiltered by ultrafiltration membrane with molecular weight cut off of 100OODa. The filtrate was freeze-dried to obtain bee venom.

 The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%:

 Phase A: glycerol 5%, propylene glycol 3%, butanediol 2%, deionized water balance.

 Phase B: Beeswax bee venom 1%, bee venom 1% (bee venom is added in the form of powder), deionized water 38%.

 Phase C: Azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.2%, phenoxyethanol/hydroxybenzyl ester/hydroxyphenylbutyl ester/hydroxyphenylethyl ester/hydroxypropylpropyl ester/hydroxyphenyl isobutyl ester 0.1 %.

 Process: 30 ° C, first mix phase A, then add pre-mixed phase B to phase A, stir and mix evenly, add phase C component, mix and prepare the bee venom with acne effect of this example The composition one contains a bee venom acne agent.

 The aqueous preparation prepared by the embodiment has no precipitation, high transparency, easy absorption, and no irritation. Example 4

 Buy bee crude bee venom from Fujian Shenfeng Technology Development Co., Ltd., add 15 times volume of water to fully dissolve, centrifuge at 6000g for 20min, and the supernatant is ultrafiltered by ultrafiltration membrane with molecular weight cut off of 100oDa. The filtrate is freeze-dried to obtain bee venom. .

 The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%:

Phase A: 0.4% of butyl rubber, 0.6% of acrylate/C _1Q _ _3Q alkyl acrylate cross-linked polymer, EDTA-2Na 0.1% glycerin 5%, propylene glycol 5%, butanediol 5%, deionized water balance.

 Phase B: Bee venom 0.5% (prepared in the form of distilled water pre-formed into a mass fraction of 10% bee venom solution), kelp polysaccharide 0.01%, pearl hydrolysate liposome 3%, deionized water 20%.

 Phase C: PEG-40 hydrogenated castor oil ester 1%, triethanolamine 0.2%, fragrance 0.1%, phenoxyethanol / methylparaben / hydroxyphenyl butyl ether / hydroxyphenyl ethyl ester / hydroxypropyl propyl ester / hydroxybenzene butyl butyl Ester 0.1%.

 Process: Mix the A phase components and heat to 80 ° C to stir evenly. When cooling to 40 ° C, add pre-mixed homogeneous phase B, stir lOmin to transparent, add phase C and mix well to obtain the embodiment. The acne-promoting bee venom composition contains a acne gel containing bee venom.

 The gel prepared in the present embodiment is transparent, finely organized, and free of agglomeration. There is no paste separation at -10 ° C and 40 ° C. Example 5

 The bee venom was prepared according to the method of preparing bee venom according to Example 1.

 The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%:

Phase A: Hansheng gum 0.2%, acrylate/C _1Q _ _3Q alkyl acrylate crosslinked polymer 0.6%, glycerin 5%, propylene glycol 3%, deionized water balance.

 Phase B: Bee venom 0.00001% (added in the form of deionized water pre-formed into 10% bee venom solution), sodium alginate 1%, laver polysaccharide 0.5%, wakame polysaccharide 0.5%, Lysimachia polysaccharide 0.5%, Cauliflower polysaccharide 0.5%, Eucheuma polysaccharide 0.5%, Sargassum polysaccharide 0.5%, Artemisia polysaccharide 0.5%, Sargassum polysaccharide 0.5%, water-soluble pearl powder 0.2%, deionized water 30%.

 Phase C: Azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.3%, phenoxyethanol/hydroxybenzyl ester/hydroxyphenylbutyl ester/hydroxyphenylethyl ester/hydroxypropylpropyl ester/hydroxyphenyl isobutyl ester 0.1 %.

 Process: Mix the A phase components and heat to 80 ° C to stir evenly. When cooling to 45 ° C, add pre-mixed homogeneous phase B, stir and homogenize for 3 min, add the C phase components in sequence at room temperature, stir evenly. The bee venom composition having the acne effect of the present embodiment is a acne gel containing bee venom.

The gel prepared in the present embodiment is light in texture and cool. Example 6 The bee venom was prepared according to the method of preparing bee venom according to Example 2.

 The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%:

 Bee venom 2% (added in the form of powder), spirulina polysaccharide 0.2%, copper algae 0.2%, Fucus polysaccharide 0.2%, carrageenan 0.2%, big leaf algae 0.2%, chlorella polysaccharide 0.2 %, nano pearl powder 5%, water soluble pearl powder 10%, almond powder 11%, honey 5%, preservative 1%, deionized water balance. The bee venom, spirulina polysaccharide, copper algae polysaccharide, fucus polysaccharide, carrageenan polysaccharide, big leaf algae polysaccharide, chlorella polysaccharide, water-soluble pearl powder are dissolved in deionized water, and all dissolved, at room temperature Add the pre-mixed nano pearl powder and almond powder, stir and mix at 50r/m for 5min, add honey and preservative, stir and mix for 5min, and obtain the bee venom composition containing acne effect of this embodiment. Bee venom acne mask. Example 7

 The bee venom was prepared according to the method of preparing bee venom according to Example 3.

 The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%:

 Phase A: Carbomer 0.1%, glycerol 5%, propylene glycol 3%, deionized water balance.

 Phase B: 1% octanoylsalicylic acid, 1.2% of cetearyl ether-2, 1.5% of cetearyl ether-21, 3% of hydrogenated polyisobutylene, 3% of caprylic/capric triglyceride, Ethylhexyl palmitate 3%, polydimethylsiloxane 2%, and hydroxyethyl ester 0.1%.

 Phase C: Bee venom 0.5% (pre-formed with deionized water in the form of 50% bee venom solution), PCA zinc 0.5%, deionized water 20%.

 Phase D: Azone/PEG-40 hydrogenated castor oil 1%, triethanolamine 0.1%, phenoxyethanol/hydroxybenzyl ester/hydroxyphenylbutyl ester/hydroxyphenylethyl ester/hydroxypropylpropyl ester/hydroxyphenyl isobutyl ester 0.1 %.

 Process: Heat phase A and phase B to 80 ° C, respectively, and slowly add phase B to phase A while stirring. Stirring to 55 ° C, adding phase C to the mixture of phase A and phase B, stirring and mixing for 10 minutes, adding phase D, mixing and homogenizing to obtain the bee venom composition of the present embodiment having a acne effect Poisonous acne lotion.

The emulsion prepared in the embodiment has a fine hand feeling, uniform coating, no separation of oil and water, and easy absorption. Example 8

 The bee venom was prepared according to the method of preparing bee venom according to Example 1.

 The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%:

 Phase A: glycerol 5%, propylene glycol 3%, butanediol 2%, deionized water balance.

 Phase B: Bee venom 0.00001% (bee venom is added in the form of powder), PCA zinc 1.5%, deionized water 30%.

 Phase C: 2% octanoyl salicylic acid, 1% azone/PEG-40 hydrogenated castor oil, 0.2% triethanolamine, phenoxyethanol/hydroxybenzyl ester/hydroxyphenylbutyl ester/hydroxyphenylethyl ester/hydroxyphenylpropyl Ester/hydroxyphenyl isobutyl ester 0.1%.

 Process: 35 °C, first mix phase A, then add pre-mixed phase B to phase A, stir and mix evenly, add phase C component, mix and prepare the bee venom with acne effect of this example The composition one contains a bee venom acne agent.

 The aqueous preparation prepared by the embodiment has no precipitation, high transparency, easy absorption, and no irritation. Example 9

 The bee venom was prepared according to the method of preparing bee venom according to Example 4.

 The bee venom composition having the acne effect of the present embodiment is composed of the following components in a total mass percentage of 100%:

Phase A: xanthan gum 0.4%, acrylate/C _1Q _ _3Q alkyl acrylate crosslinked polymer 0.6%, EDTA-2Na 0.1% glycerin 5%, propylene glycol 5%, butanediol 5%, deionized water margin.

 Phase B: Bee venom 2% (prepared in distilled water with 10% bee venom solution), PCA zinc 0.01%, deionized water 20%.

 Phase C: octanoylsalicylic acid 0.1%, PEG-40 hydrogenated castor oil ester 1%, triethanolamine 0.2%, perfume 0.1%, phenoxyethanol/hydroxybenzyl ester/hydroxyphenylbutyl ester/hydroxyphenylethyl ester/hydroxyl Phenylpropyl ester / hydroxyphenyl isobutyl ester 0.1%.

 Process: Mix the A phase components and heat to 80 ° C to stir evenly. When cooling to 40 ° C, add pre-mixed homogeneous phase B, stir lOmin to transparent, add phase C and mix well to obtain the embodiment. The acne-promoting bee venom composition contains a bee venom acne gel.

The gel prepared in this embodiment is transparent, finely organized, and free of agglomeration. There is no paste separation at -10 ° C and 40 ° C. Example 10

 The following samples were tested in animals:

 The percentages of this example refer to mass percentages.

 The wasp bee venom and bee venom of this example were prepared in accordance with the preparation method of Example 3. Saline (0.9% NaCl solution);

 Crude bee venom composition (ie sample A, crude bee venom content is 2% by mass):

 A1: Crude bee venom 2%, normal saline 98%;

 A2: Crude bee venom 0.5%, bee crude bee venom 1.5%, normal saline 98%.

 Containing bee venom composition (ie sample B, bee venom content is 2% by mass):

 B1: 2% of wasp bee venom and 98% of normal saline;

 B2: Vespa bee venom 0.5%, bee venom 1.5%, normal saline 98%.

 Containing seaweed polysaccharide and pearl powder composition (ie sample C, total content of seaweed polysaccharide, pearl powder is 2% by mass):

 C1: kelp polysaccharide 1%, nano pearl powder 1%, physiological saline 98%;

 C2: Sargassum polysaccharide 0.5%, sodium alginate 0.5%, water-soluble pearl powder 1%, physiological saline 98%;

C3: spirulina polysaccharide 0.5%, big leaf algae polysaccharide 0.5%, chlorella polysaccharide 0.5%, water soluble pearl powder 0.5%, physiological saline 98%;

 C4: Laver polysaccharide 0.5%, Lysimachia polysaccharide 0.5%, Sargasso polysaccharide 0.5%, pearl hydrolysate liposome 0.5%, normal saline 98%.

 Containing bee venom, seaweed polysaccharide and pearl powder composition (ie sample D, bee venom, seaweed polysaccharide, pearl powder total content of 2% by mass):

 D1: Wasp bee venom 0.5%, kelp polysaccharide 0.5%, nano pearl powder 1%, physiological saline 98%;

D2: bee venom 0.3%, wasp bee venom 0.2%, Sargassum polysaccharide 0.3%, sodium alginate 0.2%, water soluble pearl powder 1%, physiological saline 98%;

 D3: honey bee venom 0.1%, spirulina polysaccharide 0.4%, big leaf algae polysaccharide 0.5%, chlorella polysaccharide 0.5%, water soluble pearl powder 0.5%, physiological saline 98%;

 D4: Wasp bee venom 1%, laver polysaccharide 0.2%, Lysimachia polysaccharide 0.2%, Sargassum polysaccharide 0.1%, pearl hydrolysate liposome 0.5%, physiological saline 98%.

Composition containing PCA zinc and octanoyl salicylic acid (ie sample E, total content of PCA zinc and octanoyl salicylic acid) For a quality score of 2%):

 El : PCA zinc 1%, octanoyl salicylic acid 1%, physiological saline 98%;

 E2: PCA zinc 1.5%, octanoyl salicylic acid 0.5%, physiological saline 98%.

 Composition containing bee venom, PCA zinc and octanoyl salicylic acid (ie sample F, total content of bee venom, PCA zinc and octanoyl salicylic acid is 2% by mass):

 F1: wasp bee venom 0.5%, PCA zinc 1%, octanoyl salicylic acid 0.5%, physiological saline 98%;

 F2: bee venom 0.4%, wasp bee venom 0.4%, PCA zinc 1%, octanoyl salicylic acid 0.2%, physiological saline 98%.

Take 102 rabbits, apply concentrated coal tar solution once a day to the opening of the ear tube in the ears of each rabbit, and apply the area to 3 _C mx3cm, 0.4mL each time, for 14 days, make rabbit ear acne The model was randomly divided into 17 groups, namely, saline group, crude bee venom composition group (sample Al-A2), bee venom composition group (sample Bl-B2), seaweed polysaccharide and pearl powder composition group. (Sample Cl-C4), bee venom, seaweed polysaccharide and pearl powder composition group (sample Dl-D4), PCA zinc and octanoyl salicylate composition group (sample El-E2), containing bee venom, PCA Zinc and octanoyl salicylate composition groups (samples F1-F2), 6 in each group.

 Corresponding samples were applied to the affected ears of rabbits in each group, about 0.5 mL each time, 3 times/d for 7 days. The therapeutic effect was observed day by day. After 7 days of the last application, the ear biopsy of the ear was taken at the ipsilateral ear and the histopathological changes were observed. According to the thickness of the epidermis and the degree of expansion of the hair follicle and the degree of keratin, the pathological changes of the specimen were divided into 4 grades: "0" is no acne; "+" is a small amount of dense keratin in the funnel of the hair follicle; "++" is A small amount of keratin is visible in the funnel of the hair follicle and extends to the sebaceous gland; "+++" is a broad keratin in the expanded hair follicle. Effectiveness = (number of "0" levels + " + "number of cases]) / number of experimental cases xl00%. The experimental results are shown in Table 1:

 Table 1: Effect of sample on rabbit ear acne model

 Histopathological status, efficiency, group, total number of cases

 Level 0 + ++ +++ (%) Saline 6 3 3 0 Contains crude bee venom combination Ai 6 3 3 50

A ₂ 6 2 4 33.3

(ie sample A)

Bee venom containing composition Bi 6 2 2 2 66.7

(ie sample B) B ₂ 6 1 4 1 83.3 containing seaweed polysaccharide and Ci 6 1 3 2 16.7 pearl powder composition c ₂ 6 2 3 1 33.3

(ie sample C) c ₃ 6 1 4 1 16.7 c ₄ 6 6 0 Containing bee venom, seaweed Di 6 3 3 100 polysaccharide and pearl powder D ₂ 6 2 3 1 83.3 Composition (ie sample D ₃ 6 1 5 100 product D) D ₄ 6 2 4 100 containing PCA zinc and Xin Ei 6 —— 1 4 1 16.7 Acyl salicylic acid combination

E ₂ 6 2 3 1 33.3 (ie sample E)

Containing bee venom, PCA

 Fi 6 2 3 1 83.3 Zinc and octyl salicyl

Acid composition (ie F ₂ 6 1 5 100 sample F ) After 7 days of saline application, there was no significant change in acne, rough skin, and sensitive grain touch on the ears. The embolization of the hair follicles of rabbit ears was visible, and inflammatory cells were visible around the sebaceous glands. Infiltrated with nodules. Compared with the saline group, the AF of the sample was more effective in improving the acne of the rabbit ear.

 The bee venom-containing composition (ie, sample B) has no significant irritation and sensitization to the skin as compared to the crude bee venom composition (ie, sample A) due to the removal of the sensitizing source in the crude bee venom. Due to the enrichment of the active ingredients in bee venom, the bee venom composition has an improved effect on rabbit acne than the crude bee venom composition.

 Compared with the composition containing bee venom (ie sample B), the composition containing seaweed polysaccharide and pearl powder (ie sample C), the composition containing bee venom, seaweed polysaccharide and pearl powder (ie sample D) on rabbit ear acne The improvement effect is more obvious, which indicates that the seaweed polysaccharide, the pearl powder and the bee venom in the present invention have a remarkable synergistic effect.

 Composition containing bee venom, PCA zinc and octanoylsalicylic acid (ie sample F) compared to the composition containing bee venom (ie sample B), containing PCA zinc and octanoylsalicylic acid (ie sample E) The effect of improving acne in rabbit ears is more obvious, which indicates that PCA zinc, octyl salicylic acid and bee venom in the present invention have significant synergistic effects.

 In summary, the bee venom composition having the acne effect of the present invention has a remarkable therapeutic effect on the rabbit ear acne model, and the drug can exhibit a remarkable effect after 4 days of administration. Example 11

The bee venom composition having the acne effect prepared in Examples 1-9 was subjected to a human trial, and the samples 1-9 were in turn the bee venom compositions having the acne effect prepared in Examples 1-9. A total of 45 volunteers with mild and moderate facial acne were selected, including 15 males and 30 females; aged 17-35 years, mean 28.3 years; duration 4 weeks-7 years, average 3.5 years, before applying cosmetics 2 weeks, no glucocorticoids, retinoids and antibiotics, no hyperlipidemia and abnormal liver and kidney function, alcoholism is prohibited, and strong sunlight or ultraviolet radiation is avoided.

 Forty-five volunteers were randomly divided into 9 groups of 5 each. After the subject cleaned the affected area with warm water and dried it, apply the corresponding sample once a night. During the use period, the other drugs or cosmetics were stopped. After 4 weeks, the therapeutic effect was determined. (The method of using the mask prepared in Example 6 is: washing the face with warm water. For the part, apply the mask to the skin of the affected part for 10 minutes, then wash it with water, once a night for 4 weeks). Before treatment and at the 4th week of treatment, the total number of facial inflammatory lesions (sum of inflammatory papules and pustules) and the total number of non-inflammatory lesions (sum of whiteheads and blackheads) were counted separately, and detailed records were used. Adverse reactions. Judging criteria: Ke Yu: The efficacy index is ≥90%; markedly effective: 60% ≤ therapeutic index <90%; progress: 20% ≤ therapeutic index <60%; invalid: efficacy index <20%. Efficacy index = (total number of lesions before treatment - total lesions after treatment) / total lesions before treatment x l00%. Effectiveness = (base number of cases + number of effective cases) / number of cases χ ΐοο %.

 After 4 weeks, the therapeutic effects of the acne-promoting bee venom compositions of Examples 1-9 on acne are shown in Table 2:

 Table 2: The therapeutic effect of the bee venom composition with acne effect on acne The more effective the sample base is, the more effective the total number of cases is. (%) Example 1 1 4 5 100 Example 2 5 5 100 Example 3 2 3 5 100 Example 4 2 3 5 100 Example 5 1 4 5 100 Example 6 3 2 5 100 Example 7 1 4 5 100 Example 8 1 3 1 5 80 Example 9 2 3 5 100 The acne-promoting bee venom composition has good compatibility with the skin, can be quickly absorbed by the skin, safe and mild, does not cause irritation, allergies, etc., does not burden the skin, but also dissolves the angle plug, reduces inflammation, and promotes rapid wounds. Heal, repair scars, and reduce pigmentation. After 4 weeks of using the acne-producing bee venom composition of the present invention, the skin returned to normal smoothness and smoothness, and the symptoms of acne were not easily relapsed after being stopped, and the acne effect was safe, remarkable, long-lasting, and comprehensive (Table 2).

In the above 45 volunteers with mild and moderate facial acne vulgaris, the number of inflammatory lesions in the original face totaled 648. The total number of non-inflammatory lesions was 997. After applying the acne-promoting bee venom composition of Examples 1-9 for 4 weeks, 553 facial inflammatory lesions were cured, and 834 non-inflammatory lesions were cured. Among them, the bee venom composition prepared in Examples 1-3 (bee venom is the main acne component) cured 160 inflammatory lesions of the face, 258 non-inflammatory lesions; the bee venom composition prepared in Examples 4-6 ( Bee venom, seaweed polysaccharide and pearl powder are the main acne ingredients), 216 facial inflammatory lesions were cured, and 275 non-inflammatory lesions; bee venom compositions prepared in Examples 7-9 (bee venom, PCA zinc and octanoyl) Salicylic acid is the main acne component). It has cured 177 facial inflammatory lesions and 301 non-inflammatory lesions. It can be seen that when the bee venom is compounded with the seaweed polysaccharide and the pearl powder, the bee venom composition having the acne effect of the invention has the effects of reducing inflammation, sunscreen, moisturizing and acne scar, and is effective for curing inflammatory damage and pustules. Sexual and cystic acne has obvious effects. When the bee venom is compounded with PCA zinc and octanoylsalicylic acid, the bee venom composition having the acne effect of the invention significantly enhances the pores and balances the secretion of oil and the like, and is effective for curing non-inflammatory damage, spotting, The effect of papular and atrophic acne is obvious.

 In summary, the acne-promoting bee venom composition of the present invention is suitable for people of different genders, ages, and skin types suffering from problems such as acne, adult acne, acne scars, or scars.

 The above-mentioned embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and modifications may be made without departing from the spirit and scope of the invention. Simplification, which should be an equivalent replacement, is included in the scope of protection of the present invention.

Claims

claims 1. A bee venom composition with anti-acne effect, including a base component of an anti-acne cosmetic, characterized in that it also contains bee venom. 2. The bee venom composition according to claim 1, characterized in that the bee venom is prepared by the following method: dissolving the crude bee venom in water, and then ultrafiltrating through an ultrafiltration membrane with a molecular weight cutoff of 10000 Da. Finally, the filtrate is freeze-dried to obtain bee venom. 3. The bee venom composition according to claim 2, characterized in that the bee venom is prepared by the following method: Take the crude bee venom and add 10-30 times the volume of water to fully dissolve it, and centrifuge at 5000-10000g for 10 -30min, the supernatant is ultrafiltrated through an ultrafiltration membrane with a molecular weight cutoff of 100OODa, and then the filtrate is freeze-dried to prepare bee venom. 4. The bee venom composition according to claim 1 or 2 or 3, characterized in that the content of the bee venom accounts for 0.00001%~2% of the total mass of the bee venom composition. 5. The bee venom composition according to claim 4, characterized in that the bee venom composition with anti-acne effect also contains seaweed polysaccharide and pearl powder, and the content of seaweed polysaccharide accounts for 10% of the total mass of the bee venom composition. 0.01-5, the content of pearl powder accounts for 0.2%-15% of the total mass of the bee venom composition. 6. The bee venom composition according to claim 5, characterized in that the seaweed polysaccharide is sodium alginate, nori polysaccharide, wakame polysaccharide, loosestrife polysaccharide, Gelidium polysaccharide, Eucheuma polysaccharide, hijiki One or a combination of polysaccharides, laminarin, seaweed polysaccharide, spirulina polysaccharide, sargassum polysaccharide, copper algae polysaccharide, fucus polysaccharide, carrageenan polysaccharide, macrophylla polysaccharide and chlorella polysaccharide; The pearl powder is one or a combination of nano pearl powder, water-soluble pearl powder, pearl hydrolyzate liposome. 7. The bee venom composition according to claim 4, wherein the bee venom composition with anti-acne effect also contains zinc PCA and capryloyl salicylic acid. 8. The bee venom composition according to claim 7, wherein the content of zinc PCA accounts for 0.01%-1.5% of the total mass of the bee venom composition, and the content of capryloyl salicylic acid accounts for 0.01%-1.5% of the total mass of the bee venom composition. 0.1%-2% of the total mass of the bee venom composition. 9. The bee venom composition according to claim 2, characterized in that the crude bee venom is wasp crude bee venom and/or bee crude bee venom. 10. The bee venom composition according to claim 1, characterized in that, the bee venom composition is a cream, lotion, aqueous solution, gel, jelly or facial mask, and the bee venom composition can For use as ordinary cosmetics or Functional cosmetics with anti-acne effect.