AU2003200420B2

AU2003200420B2 - Inhibitory, Monoclonal antibody against blood clotting factor VII-activating protease

Info

Publication number: AU2003200420B2
Application number: AU2003200420A
Authority: AU
Inventors: Annette Feussner; Christian Kannemaier; Wiegand Lang; Gudrun Muthnaumann; Fumie Nakazawa; Klaus Preissner; Juergen Roemisch; Hans-Arnold Stoehr
Original assignee: CSL Behring GmbH Deutschland
Current assignee: CSL Behring GmbH Deutschland
Priority date: 2002-02-08
Filing date: 2003-02-07
Publication date: 2010-01-28
Anticipated expiration: 2023-02-07
Also published as: KR100972943B1; JP4398648B2; ATE323107T1; ES2259114T3; CA2418411C; DE50302926D1; KR20030067588A; EP1334983A3; DE10205520A1; AU2003200420A1; EP1334983A2; EP1334983B1; US7829095B2; CA2418411A1; JP2003286300A; DK1334983T3; US20030215447A1

Abstract

Monoclonal antibody (MAb) that inhibits factor 7 activating protease (FSAP), or its proenzyme, that activates blood coagulation factor VII (F7), is new. Independent claims are also included for the following: (1) Hybridoma cell lines that produce MAb; (2) Epitope (E) that binds to the antibody (MAb') produced by hybridoma DSM ACC 2533; (3) Monoclonal antibodies that bind to (E); (4) Peptides that competitively inhibit binding of MAb' to FSAP; (5) Single-chain antibodies (SAb) and humanized antibodies that have the binding specificity of MAb'; (6) Stabilized FSAP or its proenzyme, inhibited by addition of MAb; (7) Blood coagulation factor VII (F7) composition that contains MAb; (8) Stabilized factor V, factor VIII or fibrinogen composition that is protected against degradation by FSAP by addition of MAb; (9) Determining activity of FSAP by treating a sample with MAb and then measuring any residual amidolytic activity; (10) Preparing FSAP or its proenzyme, using MAb as immunoadsorbent; and (11) Preparing FSAP in which MAb is added to the starting material from which FSAP is recovered.

Description

AUSTRALIA Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Application Number: Lodged: Invention Title: INHIBITORY, MONOCLONAL ANTIBODY AGAINST BLOOD CLOTTING FACTOR VII-ACTIVATING PROTEASE The following statement is a full description of this invention, including the best method of performing it known to :- us Inhibitory, monoclonal antibody against blood clotting 5 factor VII-activating protease The invention relates to a monoclonal antibody which inhibits specifically the factor VII-activating protease or its proenzyme but which does not impair the 10 proteolytic properties of other proteases. It is known that proteases have a crucial function in the release of active enzymes from their precursors, the proenzymes, in the organism. In addition, however, 15 proteases also have the property of degrading activated enzymes again which therefore frequently become inactive again after only a short time. In order to prepare stabilized pharmaceutical preparations, it is therefore frequently necessary to inhibit the activity 20 of specific proteases. The stabilization against degradation caused by proteases is therefore an important task also in the case of pharmaceutical preparations containing blood 25 clotting factors, in order to ensure the effectiveness of such preparations, in particular even after a relatively long storage period. The blood clotting system comprises two different, 30 cascade-like activation pathways of clotting factors present in the plasma. Depending on the triggering mechanism, either the endogenous or the exogenous route preferably serves to initiate clotting. 35 In a tissue injury, thromboplastin (tissue factor, TF) is exposed on surfaces as an initiator of the exogenous clotting route. The clotting factor VII (FVII), as well as circulating, activated FVII (FVIIa), is bound to the membrane-bound thromboplastin. In the presence of -2 calcium ions and lipids, this TF-FVIIa complex leads to the binding of FX which is converted into its activated form (FXa) by limited proteolysis. By activation of prothrombin to thrombin, FXa in turn leads to the 5 formation of fibrin and thus finally to wound closure. FVIIa is found in very low concentrations in the plasma of healthy people. As yet, only very little is known about the cause and origin of the FVIIa circulating in 10 the blood. Traces of expressed thromboplastin or thromboplastin released during cell destruction could play a part here. The German laid-open specification 199 03 693 discloses 15 an FVII-activating protease which is also referred to as FSAP (Factor Seven-Activating Protease). Owing to this property, FSAP can accelerate blood clotting and be applied as a pharmaceutical to hemorrhagic complications. 20 FSAP is present in the blood plasma as a proenzyme (single-chain FSAP, scFSAP). Using known chromatographic methods, it is possible to obtain mainly only the two-chain activated form of the 25 protease (two-chain FSAP, tcFSAP), since the proenzyme is activated during the preparation. This activation may be caused by other proteases such as urokinase but may also occur autocatalytically. Activated FSAP can inactivate itself proteolytically. The preparation of 30 scFSAP, in particular, is therefore difficult, but preparation of the intact tcFSAP is also not simple. Optimized methods for preparing FSAP have been described in the German laid-open specifications 139 37 218 and 139 37 219. An important method step in 35 this connection is immunoadsorption to matrix-coupled, monoclonal antibodies and specific conditions for stabilizing the eluate. However, in immunoadsorption too, care must be taken to work as quickly as possible in order to avoid the formation of even small amounts -3 of tcFSAP. This is most successfully done by using specific monoclonal antibodies which prevent or reduce only the activation or autoactivation of FSAP instead of polyvalent protease inhibitors such as aprotinin, 5 Cl-esterase inhibitor or a-2 antiplasmin in the preparation. The invention therefore relates to a monoclonal antibody which inhibits the blood clotting factor VII 10 activating protease or its proenzyme. Particularly suitable for this purpose is the monoclonal antibody produced by hybridoma cell line DSM ACC 2533. If such a monoclonal antibody is added to blood clotting factor VII-activating protease or to its proenzyme, the latter 15 are inhibited thereby. A factor VII preparation containing the abovementioned monoclonal antibody is stabilized due to inhibition of the factor VII activating protease and its proenzyme. 20 The antibody of the invention may also be used for preparative and analytical applications. In fact, the German laid-open specification 199 03 693 discloses that FSAP has the property of inactivating during incubation the blood clotting factors VIII/VIIIa and 25 V/Va in a manner which depends on the protease concentration and the incubation time. If the FSAP action is inhibited by the addition of the antibody of the invention, then this protects said blood clotting factors from proteolytic degradation. It is also 30 possible to stabilize fibrinogen solutions by adding the antibody of the invention. The inhibitory antibody of the invention opens up possible diagnostic uses by the fact that it is 35 possible, when adding it to a protease-containing solution, to detect via selective inhibition of FSAP, whether the proteolytic action can be attributed to FSAP or to a different protease. This method may be applied in particular in all assay systems which are -4 based on FSAP activity, for example the cleavage of chromogenic substrates, by activated FSAP or by measuring the amidolytic activity of FSAP-activated urokinase. 5 In addition, the inhibitory antibodies of the invention may also have direct prophylactic or therapeutic uses in the case of particular disorders. Thus, for example, an increase in the FSAP content in the plasma can 10 increase the procoagulant properties of the blood and thus the risk of thrombosis. Administering an inhibitory antibody can reduce the coagulability of the blood and thus reduce the risk of thrombosis. 15 German laid-open specification 19 903 693 also mentions the particular fibrinolytic action of pharmaceutical preparations containing the blood factor VII-activating protease. Said protease may therefore also be used for the treatment of disorders caused by fibrin-containing 20 thrombi. From this it follows that higher concentrations of FSAP may initiate or increase hemorrhagic tendencies which can be prevented or reduced by an inhibitory monoclonal antibody. This may also inhibit adverse effects on wound healing or the 25 development of cancer. The large variety of described possible applications of an inhibitory monoclonal antibody against FSAP are achieved by said antibody preventing activation of 30 scFSAP and thus subsequent formation of tcFSAP. Since many of the previously known properties of FSAP are mediated by tcFSAP, inhibition of scFSAP is sufficient in order to block FSAP actions. 35 The monoclonal antibody obtained according to the invention from hybridoma cells DSM ACC 2533, therefore very effectively inhibits tcFSAP activity but also scFSAP autoactivation. Of course, this monoclonal antibody must still be humanized prior to application -5 as a prophylactic or therapeutic agent in humans. These methods are well known and are not intended to be described here in detail. 5 Moreover, German patent application 100 52 319.6 has already described that, besides the blood clotting factor VII-activating protease (FSAP), in 5 to 10% of all blood donors tested there is an FSAP mutant which is inactive with respect to scuPA activation and whose 10 amino acid sequence exhibits a Glu/Gln exchange and Gly/Glu exchange at amino acid position 393. This single-nucleotide polymorphism (=SNP) is associated with a reduction in the activation of proplasminogen activators and thus with a reduced fibrinolytic 15 potential and with an increased risk of thrombosis. In order to reduce this. risk, European patent application 01 115 691.6 proposes the administration of wildtype FSAP. 20 The present invention now provides the further possibility of inhibiting FSAP and thus also the mutant by administering an inhibitory monoclonal antibody, thereby reducing the thrombotic or thromboembolic potential. On the other hand, FSAP mutants whose FVII 25 activating properties are reduced but whose fibrinolytic capacity is increased may be used for the treatment of potentially increased hemorrhagic tendencies by administering a monoclonal, inhibitory antibody of the invention. 30 The hybridoma cell line DSM ACC 2533 was identified and prepared as follows: 3 mice were immunized with FSAP. The spleen cells from one mouse were fused with the murine myeloma cell line SP2/0-Ag 14, using 35 polyethylene glycol 4,000 as fusion reagent. The cells were distributed onto 24-well culture plates. The medium used was Dulbecco mod. Eagle's Medium containing 10% fetal calf serum and HAT for selection. After approximately 2 weeks, the growing cell clones were -6 transferred to the wells of a 48-well culture plate and coded. The supernatant of 1,728 cell clones grown was assayed for the presence of mouse IgG by means of an ELISA. Mouse-IgG-positive supernatants were tested for 5 specificity with the aid of immobilized FSAP. 108 of the clones assayed were identified as being specific for FSAP. Further experiments reviewed the inhibitory property of the monoclonal antibody from hybridoma cell line DSM ACC 2533. The antibody is of the IgGl type. 10 The monoclonal antibody from hybridoma cell line DSM ACC 2533 inhibits the proteolytic activity of tcFSAP. Accordingly, not only does it inhibit the ability of FSAP to activate factor VII or prourokinase but also 15 prevents FSAP autoactivation. The following examples further illustrate the invention: -7 Example 1 Studying the effect of the monoclonal antibody from DSM ACC 2533 on (auto)activation of scFSAP 5 Recalcification of citrate plasma activates coagulation and finally leads to the formation of a fibrin clot. Under these circumstances, scFSAP is activated only after a long delay, if at all. By simulating "contact 10 activators" or reactive surfaces via the addition of dextran sulfate, scFSAP is also activated in the plasma milieu. This can be illustrated very clearly on the basis of SDS-PAGE and Western blot analyses. For this purpose, the recalcified sample to which dextran 15 sulfate has been added is incubated for different times and then subjected to a "euglobulin" precipitation familiar to- the skilled worker, in order to be able to carry out the subsequent SDS-PAGE in the best possible way. In this way, the protein concentration of the 20 samples is lowered and the smearing of band patterns is prevented. A reducing agent is added to the samples obtained in this way, in order to identify more easily the heavy and light tcFSAP chains resulting from scFSAP activation. After transferring the band patterns 25 obtained by SDS-PAGE to nitrocellulose (blotting), the labeled, monoclonal antibodies DSM ACC 2453 and DSM ACC 2454 described in German patent application 100 36 641.4 are used for detecting the two tcFSAP bands. 30 As a result of this experiment, the disappearance of scFSAP on the blot and the increase in the two tcFSAP chains are observed. Occasionally, it is also possible that only the heavy chain becomes visible, since the 35 tcFSAP light chain contains the active site of FSAP and, during the incubation of the plasma, reacts with corresponding inhibitor proteins such as Cl-esterase inhibitor or a-2 antiplasmin and finally, as a (quasi -8 covalent) complex, is recognized by the detecting, monoclonal antibody with more difficulty. If the monoclonal antibody obtained from DSM ACC 2533 5 is added to the above-described plasma sample and if scFSAP activation is detected as described above, disappearance of the high molecular weight (one-chain form) band and, correspondingly, appearance of the heavy (and light) chain(s) of tcFSAP are not observed, 10 in contrast to the control (without said monoclonal antibody or with a noninhibitory, monoclonal antibody). Thus, the monoclonal antibody from the hybridoma cell line of DSM ACC 2533 has bound to scFSAP, thereby preventing scFSAP activation. 15 The titration of this effect indicates that said antibody is a highly potent, monoclonal antibody for inhibiting scFSAP activation. Even amounts equimolar to FSAP significantly reduce activation, and this renders 20 this inhibitory, monoclonal antibody a very valuable diagnostic and preparative excipient. The high effectivity at relatively low concentration makes a potential use of this monoclonal antibody for administration in humans attractive. 25 Example 2 Inhibition of the active tcFSAP by the inhibitory, monoclonal antibody from hybridoma cell line DSM ACC 30 2533 As described in the German patent application 199 03 693 the activity of FSAP may be tested in various assay systems. In addition to the activation of 35 plasminogen-activator proenzymes or of FVII with corresponding acceleration of coagulation, it is also possible, for example, to monitor the rate of inactivation of FV, FVIII, FIX or fibrinogen. In these - 9 assay systems, the inhibitory, monoclonal antibody of the invention was tested for its inhibitory properties. The result is that the inhibitory, monoclonal antibody 5 from hybridoma cell line DSM ACC 2533 inhibits in a potent manner the activity of tcFSAP toward the abovementioned blood clotting factor. This is also true for scFSAP whose activation to tcFSAP and thus its conversion to the amidolytically active form are 10 prevented, as is shown in Example 1 above. Hybridoma cell line DSM ACC 2533 is deposited at DSMZ DEUTSCHE SAMMLUNG VON MIKROORGANISMEN UND ZELLKULTUREN GmbH, Mascheroder Weg -lb, D-38124 Braunschweig.

Claims

1. A monoclonal antibody which inhibits blood clotting factor VII-activating protease (FSAP) and its proenzyme. 10

2. The monoclonal antibody as claimed in claim 1, which is produced by hybridoma cell line DSM ACC

2533.

3. A hybridoma cell line, which produces an antibody 15 as claimed in claim 1.

4. The hybridoma cell line as claimed in claim 3, which is the hybridoma cell line deposited under accession number DSM ACC 2533. 20 5. The monoclonal antibody as claimed in claim 1, whose binding to FSAP is inhibited by the monoclonal antibody as claimed in claim 2. 25 6. A single-chain antibody, which has the binding specificity of the antibody as claimed in claim 2. 7. The antibody as claimed in claim 6, which is bound to a carrier protein, preferably human serum 30 albumin or human transferin. 8. A humanized antibody, which has the binding specificity of the antibody as claimed in claim 2. 35 9. A factor VII preparation, which contains a monoclonal antibody as claimed in either of claims 1 and 2, which antibody inhibits the factor VII activating protease and its proenzyme. - 11 10. A stabilized factor V, factor VIII or fibrinogen preparation, which is protected against proteolytic degradation by factor VII-activating protease due to inhibition of said protease by 5 means of a monoclonal antibody of either of claims 1 and 2. 11. A pharmaceutical preparation for reducing the coagulability of blood, which contains a 10 monoclonal antibody of either of claims 1 and 2. 12. A method for determining the activity of blood clotting factor VII-activating protease, wherein the amount of proteases contained in the sample to 15 be studied is stabilized by the addition of a sufficient amount of a monoclonal antibody of either of claims 1 and 2 and the amidolytic activity remaining is measured only thereafter. 20 13. A method for preparing FSAP or the FSAP proenzyme, which uses the monoclonal antibody of any one of claims 1, 2, 5, or 6 to 8 as immunoadsorbent. 14. A method for preparing FSAP or the FSAP proenzyme, 25 wherein the monoclonal antibody of any one of claims 1, 2, 5, or 6 to 8, is added to the starting material from which FSAP is obtained. 15. The use of the monoclonal antibody of claim 1 or 2 30 for preventing or reducing the risk of thrombosis and the coagulability of blood which has an increased FSAP content or FSAP proenzyme content. 16. The use of the monoclonal antibody of claim 1 or 2 35 for the treatment of disorders caused by fibrin containing thrombi. 17. The use of the monoclonal antibody of any one of claims 1, 2, 5, or 6 to 8, for the manufacture of - 12 a medicament for preventing or reducing the risk of thrombosis and the coagulability of blood which has an increased FSAP content or FSAP proenzyme content.

5 18. The use of the monoclonal antibody of any one of claims 1, 2, 5, or 6 to 8, for the manufacture of a medicament for the treatment of disorders caused by fibrin-containing thrombi. 10 19. A method of prophylaxis or treatment of thrombosis, or disorders caused by fibrin containing thrombi, in a subject comprising administering to the subject an efficacious amount 15 of an antibody of any one of claims 1, 2, 5, or 6 to 8, or a pharmaceutical preparation of claim 11.