AU2007200813B2 - Novel hydrogel isolated cochleate formulations, process of preparation and their use for the delivery of biologically relevant molecules - Google Patents

Description

P/00/0 i i Regulation 3.2 AUSTRALIA Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT (ORIGINAL) Name of Applicants: Biodelivery Sciences, Inc. of UMDNJ New Jersey Medical School, 185 So Orange Avenue, Newark, NJ 07103, United States of America AND University of Medicine and Dentistry of New Jersey of 30 Bergen Street, University Heights, Newark, NJ 07103-3000, United States of America Actual Inventors: ZARIF, Leila; JIN, Tuo; SEGARRA, Ignacio; and MANNINO, Raphael Address for Service: DAVIES COLLISON CAVE, Patent & Trademark Attorneys, of 1 Nicholson Street, Melbourne, 3000, Victoria, Australia Ph: 03 9254 2777 Fax: 03 9254 2770 Attorney Code: DM Invention Title: "Novel hydrogel isolated cochleate formulations, process preparation and their use for the delivery of biologically relevant molecules" The following statement is a full description of this invention, including the best method of performing it known to us:- NOVEL HYDROGEL ISOLATED COCHLEATE FORMULATIONSPROCESSOFPREPARATION AND TIEIR USE FORTE DELIVERY OF BIOLOGICALLY RELEVANT MOLECULES FIELDOF THE INVFNnION This application is a divisional of Austmlian Application No. 2004200967, the entire contends of which 5 are incorporated herein by efemnce. The present invention relates to a novel process for poparing a novel lipid-based cochleate delivery system, the phannaceutical preparations delved from t lipid-based cochleate delivery system, and the use of these pharmaceutical prepamrations to achieve efficient systemic and mucosal delivery of biologically relevant molecles. BACKGROUND OF TIE INVENTION 10 The ability of biologically relevant molecules to be administered via the oral route depends on several factors. The biologically relevant molecule must be soluble in the gastrointestinal fluids in order for the biologically relevant molecule to be transported across biological membranes for an active transpon mechanism, or have suitable small particle size that can be absorbed through the Peyer's Patches in the small intestine and through the 15 lymphatic system. Particle size is an important parameter when oral delivery is to be achieved (see Couvreur P. et al, Adv. Drug Delivery Reviews 10:141-162, 1993). The primary issue in the ability to deliver drugs orally is the protection of the drug from proteolytic enzymes. An ideal approach is to incorporate the drug in a hydrophobic material so that the aqueous fluids cannot penetrate the system. Lipid-based cochleates are an 20 ideal system that can achieve this purpose. The advantages of cochleates are numerous. The cochleates have a nonaqueous structure and therefore they: a) are more stable because of less oxidation of lipids; b) can be stored lyophilized, which provides the potential to be stored for long periods of time at room temperatures, which would be advantageous for worldwide shipping and storage prior to administration; c) maintain their structure even after lyophilization, whereas liposome structures are 5 destroyed by lyophilization; d) exhibit efficient incorporation of biologically relevant molecules into the lipid bilayer of the cochleate structure; e) have the potential for slow release of a biologically relevant molecule in vivo as cochleates dissociate; 10 f) have a lipid bilayer which serves as a carter and is composed of simple lipids which are found in animal and plant cell membranes, so that the lipids are non toxic; g) are produced easily and safely; h) can be produced as defined formulations composed of predetermined amounts and 15 ratios of drugs or antigens. Cochleate structures have been prepared first by D. Papahadjopoulos as an intermediate in the preparation of large unilamellar vesicles (see US 4,078,052). The use of cochleates to deliver protein or peptide molecules for vaccines has been disclosed in US 5,840,707. However, neither of these patents addresses the importance of particle size or the 20 effective oral delivery of biologically relevant molecules mediated by small-sized cochleates. 2 SUMMARY OF THE INVENTION Accordingly, it is an object of this invention to provide a method for obtaining a hydrogel-isolated cochleate of a particle size less than one micron. The method further comprises the steps required to encochlicate at least one biologically relevant molecule in the 5 hydrogel-isolated cochleates in a therapeutically effective amount. A "biologically relevant molecule" is one that has a role in the life processes of a living organism. The molecule may be organic or inorganic, a monomer or a polymer, endogenous to a host organism or not, naturally occurnng or synthesized in vitro, and the like. Thus, examples include vitamins, minerals, amino acids, toxins, microbicides, 10 microbistats, co-factors, enzymes, polypeptides, polypeptide aggregates, polynucleotides, lipids, carbohydrates, nucleotides, starches, pigments, fatty acids, hormones, cytokines, viruses, organelles, steroids and other multi-ring structures, sacchandes, metals, metabolic poisons, drugs, and the like. These and other objects have been obtained by providing an encochleated biologically 15 relevant molecule, wherein the biologically relevant molecule-cochleate comprises the following components: a) a biologically relevant molecule, b) a negatively charged lipid, and c) a cation component, 20 wherein the panicle size of the encochleated biologically relevant molecule is less than one micron, and further wherein the biologically relevant molecule is preferably a drug. 3 The present invention further provides a method of orally administering to a host a biologically effective amount of the above-described cochleate. In a preferred embodiment, the biologically relevant molecule-cochleate is administered orally. 5 BRIEF DESCRIPTION OF THE FIGURES Figure 1. Schematic of the process by which the hydrogel-isolated cochleates with or without drug are obtained. Figures 2a and 2b. Particle size distribution (weight analysis) of hydrogel isolated cochleates either loaded with amphotericin B (AmB) (fig 2a) or empty (fig 2b) as measured by laser 10 light scattering. Figures 3a and 3b. Microscopic images of a mixture of liposomes in dextran dispersed into PEG gel solution. The small black dots are dextran particles formed by dispersing the dextran phase in the PEG phase. The large open circles are fo-ned by fusion of small dextran particles. Partition of liposomes favors the dextran phase as indicated by the yellow color of 15 AmB. 3b. Microscopic images of the sample shown in Fig. 3a after treatment with CaC 2 solution. The black objects in circles, indicated by an arrow, are cochleates formed by the addition of Ca 2 ions. Figures 4a-4f. Microscopic images of the sample shown in Figs. 3a and 3b after washing with a buffer containing I m.M CaC 2 and 100 mM NaCl. Aggregates are formed by the Cochleate 20 particles. 4b. Suspension shown in Fig. 4a following the addition of EDTA. Cochleate particles opened to liposomes with a diameter of 1-2 microns, indicating the intrinsic size of the cochleate particles is in sub-micron range. 4c. AmB hydrogel isolated-cochleates precipitated with zinc according to the procedure described in Example 4. 4d. Cochleates 4 displayed in fig 4c after treatment with EDTA. 4e. Empty hydrogel isolated-cochleates precipitated with zinc according to the procedure described in Example 3. 4) cochleates displayed in 4f aftcr treatment with EDTA Figure 5. Micrographs of hydrogel-isolated cochleates after freeze fracture. 5 Figure 6. Growth inhibition of Candida albicans by hydrogel-isolated cochleates loaded with A.mB at 0.625 pg AmB/ml Comparison is made to AmB in DMSO and AnmBisome R. Figure 7. Effect of hydrogel-isolated cochleates on the viability of Candida albicans after 30 hours. Figure 8. Efficacy of Amphotenicin B-cochleates and macrophage cultures. 10 Figure 9. Amphotenicin B tissue levels after administration of Amphotencin B-cochleates Figure 10. Time profile tissue concentration of ArmB after single dose administration of hydrogel-isolated cochleates loaded with AmB. Figure 11. AmB tissue level 24 hrs after single dose and 24 hrs after a multiple dose regime. Figure 12. Correlation between Amphotericin B tissue level and the level of Candida albicans 15 after administration of Amphotencin B cochleates. DETAILED DESCRIPTION OF THE INVENTION The present invention provides a solution to achieve effective oral delivery of drugs by producing small-sized cochlcates using a new process. The new approach is based on the incompatibility between two polymer solutions, both of which are aqueous. Aqueous two 20 phase systems of polymers are well used for protein purification due to a number of advantages such as freedom from the need for organic solvents, mild surface tension and the biocompatibility of aqueous polymers (see P.A. Albertsson in "Partition of cell particles and 5 macromolecules", 3 rd cd:tion, Wiley NY 1 986; and "Separation using aqueous Phase System" D. Fisher Eds, Plenum NY, 1989). It is known, for example, that large polar molecules such as proteins partition to a much higher concentration in a polymer phase with the physical characteristics similar to those of dextran than in a polymer phase with the physical 5 characteristics similar to those of PEG (D. Forciniti, C. K. Hall, M. R. Kula, Biotechnol. Biocng. 38, 986 1991). According to the present invcnion there are provided processes for preparing small sized, lipid-based cochleate particles and preparations derived therefrom, comprising a biologically relevant molecule incorporated into the particles. The cochleate particles are 10 formed of an alternating sequence of lipid bilayers/cation. The biologically relevant molecule is incorporated either in the lipid bilayers or in the interspace between the lipid bilayers. One of the processes for prepanng the small-sized cochleates comprises: 1) prepanng a suspension of small unilamellar liposomes or biologically relevant molecule-loaded liposomes, 2) mixing the liposome suspension with polymer A, 3) adding, preferably by 15 injection, the liposome/Polymer A suspension into another polymer B in which polymer A is nonrniscible, leading to an aqueous two-phase system of polymers, 4) adding a solution of cation salt to the two-phase system of step 3, such that the cation diffuses into polymer B and then into the particles comprised of liposome/polymer A allowing the formation of small sized cochleates, 5) washing the polymers out and resuspending the empty or drug-loaded 20 cochleates into a physiological buffer or any appropriate pharmaceutical vehicle. A second process for preparing the small-sized cochleates comprises detergent and a biologically relevant molecule and cation. The process comprises the following steps: a) providing an aqueous suspension containing a detergent-lipid mixture; 6 b) mixing the detergent-lipid suspension with polymer A, c) adding the detergeni-lipid/polymer A suspension into a solution comprising polymer B, wherein polymer A and polymer B are immiscible, thereby creating a two-phase polymer system; 5 d) adding a solution of a cationic moiety to the two-phase polymer system, and e) washing the two-phase polymer system to remove the polymer. A lyophilization procedure can be applied and the lyophilized biologically relevant molecule-cochleate complex can be filled into soft or hard gelatin capsules, tablets or other dosage form, for systemic, dermal or mucosal delivery. 10 This process leads to a small-sized particle with a narrow size range that allows efficient oral delivery of biologically relevant molecules. The biologically relevant molecule partitions into either or both lipid bilayers and interspace, and the biologically relevant molecule is released from the cochleate particles by dissociation of the particles in vivo. Alternative routes of administration may be systemic such as intramuscular, subcutaneous or 15 intravenous or mucosal such as intranasal, intraocular, intravaginal, intraanal, parenteral or intrapulmonary. Appropriate dosages are determinable by, for example, dose-response experiments in laboratory animals or in clinical trials and taking into account body weight of the patient, absorption rate, half-life, disease severity and the like. The number of doses, daily dosage and course of treatment may vary from individual to individual. Other delivery 20 routes can be dermal, transdermal or intradermal. The first step of the new process of the present invention, which is the preparation of small liposomes, can be achieved by standard methods such as sonication or microfluidization or other related methods (see for example Liposome Technology, Liposome 7 Preparation and Related Techniques, Edited by Grcgory Gregonadis, Vol 1, 2nd Edition, CRC Press, 1993). The addition, preferably by injection, of polymer A/liposome suspension into polymer B can be achieved mechanically by using a syringe pump at an appropriate controlled rate, 5 for example a rate of 0.1 ml/min to 50 ml/min and preferably at a rate of I to 10 ml/min. The lipids of the present invention are non-toxic lipids and include, but are not limited to simple lipids which are found in animal and plant cell membranes. Preferably the lipid is a negatively charged lipid, more preferably a negatively charged phospholipid, and even more preferably a lipid from the group of phosphatidylserine, phosphatidylinositol, phosphatidic 10 acid, and phosphatidyl glycerol. The lipids may also include minor amounts of zwittenonic lipids, cationic lipids or neutral lipids capable of forming hydrogen bonds to a biologically relevant molecule such as PEGylated lipid. The polymers A and B of the present invention can be of any biocompatible polymer classes that can produce an aqueous two-phase system. For example, polymer A can be, but 15 is not limited to, dextran 200,000-500,0M0, Polyethylene glycol (PEG) 3,400-8,000; polymer B can be, but is not limited to, polyvinylpyrrolidone (PVP), polyvinylalcohol (PVA), Ficoll 30,000-50,000, polyvinyl methyl ether (PVMB) 60,000-160,000, PEG 3,400-8,000. The concentration of polymer A can range from between 2-20% w/w as the final concentration depending on the nature of the polymer. The same concentration range can be applied for 20 polymer B. Examples of suitable two-phase systems are Dextran/PEG, 5-20% w/w Dextran 200,000-500,000 in 4-10% w/w PEG 3,400-8,000; Dextran/PVP 10-20% w/w Dextran 200,000-500,000 in 10-20% w/w PVP 10,000-20,000; Dextran/PVA 3-15% w/w Dextran 200,000-500,000 in 3-15% w/w PVA 10,000-60,000; Dextran/Ficoll 10-20% w/w Dextran 8 200.000-500,000 in 10-20% w/w Ficoli 30,000-50,000, PEG/PVMNIE 2-10% w/w PEG 3,500 35,000 in 6-15% w/w PVME 60,000-160,000. The biologically relevant molecule may be an organic molecule that is hydrophobic in aqueous media. The biologically relevant molecule may be a drug, and the drug may be an 5 antiviral, an anesthetic, an anti-infectious, an antifungal, an anticancer, an immunosuppressant, a steroidal anti -inflammatory, a non-steroidal anti-inflammatory, a tranquilizer or a vasodilatory agent. Examples include Amphotericin B, acyclovir, adriamycin, cabamazepine, melphalan, nifedipine, indomethacin, naproxen, estrogens, testosterones, steroids, phenytoin, ergotamines, cannabinoids rapamycin, propanidid, 10 propofol, alphadione, echinomycine, miconazole nitrate, teniposide, taxol, and taxotere. The biologically relevant molecule may be a polypeptide such as cyclosporin, angiotensin 1, 11 and III, enkephalins and their analogs, ACTH, anti-inflammatory peptides I, II, 111, bradykirun, calcitonin, b-endorphin, dinorphin, leucokinin, leutirizing hormone releasing hormone (LHRH), insulin, neurokinins, somatostatin, substance P, thyroid releasing 15 hormone (TRIH) and vasopressin. The biologically relevant molecule may be an antigen, but the antigen is not limited to a protein antigen. The antigen can also be a carbohydrate or a polynucleotide such as DNA. Examples of antigenic proteins include envelope glycoproteins from influenza or Sendai viruses, animal cell membrane proteins, plant cell membrane proteins, bacterial membrane 20 proteins and parasitic membrane protein. The biologically relevant molecule is extracted from the source particle, cell, tissue, or organism by known methods. Biological activity of biologically relevant molecules need not be maintained. However, in some instances (e.g., where a protein has membrane fusion or 9 ligand binding activity or a complex conformation which is recognized by the immune sys tem), it is desirable to maintain the biological activity. In these instances, an extraction buffer containing a detergent which does not destroy the biological activity of the membrane protein is used. Suitable detergents include ionic detergents such as cholate salts, 5 deoxycholate salts and the like or heterogeneous polyoxyethylene detergents such as Tween, BRAG or Triton. Utilization of this method allows reconstitution of antigens, more specifically proteins, into the liposomes with retention of biological activities, and eventually efficient association with the cochleates. This avoids organic solvents, sonication, or extreme pH, 10 temperature, or pressure all of which may have an adverse effect upon efficient reconstitution of the antigen in a biologically active form. Hydrogel-isolated cochleates may contain a combination of various biologically relevant molecules as appropriate. The formation of small-sized cochleates (with or without a biologically relevant 15 molecule) is achieved by adding a positively charged molecule to the aqueous two-phase polymer solution containing liposomes. In the above procedure for making cochleates, the positively charged molecule can be a polyvalent cation and more specifically, any divalent cation that can induce the formation of a cochleate. In a preferred embodiment, the divalent cations include Ca-, Zn", Ba~ and Mg- or other elements capable of forming divalent ions 20 or other structures having multiple positive charges capable of chelating and bridging negatively charged lipids. Addition of positively charged molecules to liposome-containing solutions is also used to precipitate cochleates from the aqueous solution. 10 To isolate the cochleate structures and to remove the polymer solution, cochleate precipitates are repeatedly washed with a buffer containing a positively charged molecule, and more preferably, a divalent cation. Addition of a positively charged molecule to the wash buffer ensures that the cochleate structures are maintamcd throughout the wash step, 5 and that they remain as precipitates. The medium in which the cochleates are suspended can contain salt such as calcium chloride, zinc chloride, cobalt chloride, sodium chloride, sodium sulfate, potassium sulfate, ammonium sulfate, magnesium sulfate and sodium carbonate. The medium can contain polymers such as Tween 80 or BRIG or Tnton. The biologically relevant molecule-cochleatc 10 is made by diluting into an appropriate biologically acceptable carter (e.g., a divalent cation containing buffer). The cochleate particles can be enteric. The cochleate particles can be placed within gelatin capsules and the capsule can be enteric coated. In the preparations of the present invention certain hydrophobic materials can be 15 added to provide enhanced absorption properties for oral delivery of biologically relevant molecules. These materials are preferably selected from the group consisting of long chain carboxylic acids, long chain carboxylic acid esters, long chain carboxylic acid alcohols and mixtures thereof. The hydrophobic materials can be added either initially to the lipid prior to the formation of liposomes or in a later step in the form of a fat vehicle such as an emulsion. 20 The skilled artisan can determine the most efficacious and therapeutic means for effecting treatment practicing the instant invention. Reference can also be made to any of numerous authorities and references including, for example, "Goodman & Gillrnan's, The

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Pharmaceutical Basis for Therapcutics", (6"' Ed . Gondman et al., eds., MacMillan Publ. Co., New York, 1980). The invention will now be described by examples which are not to be considered as limiting the invention. In the examples, unless otherwise indicated, all ratios, percents and 5 amounts are by weight. EXAMPLES Example 1. Preparation of empty hydrogel-isolated cocbleates from dioleoylphosphatidylserine precipitated with calcium 10 Step I . Preparation oj small unilamellar vesiclesfrom dioleoylphosphatidylserine. A solution of diolcoyl phosphatidylsenne (DOPS, Avanti Polar Lipids, Alabaster, AL, USA) in chloroform (10 mg/ml) was placed in a round-bottom flask and dned to a film using a Buchi rotavapor at 35 1C. The rotavapor was stenlized by flashing nitrogen gas through a 0.2 pm filter. The following steps were camed out in a sterile hood. The dned lipid film was 15 hydrated with de-ionized water at the concentration of 10 mg lipid/ml. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a cooled bath sonicator (Laboratory Supplies Com., Inc.). Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear (suspension A) and there were no liposomes apparently visible under a phase contrast 20 microscope with a IOOX magnification. Laser light scattering (weight analysis, Coulter N4 Plus) indicates that the mean diameter is 35.7 + 49.7 nm. Step 2 : Preparation of hydrogel isolated cochleates The liposome suspension obtained in step I was then mixed with 40 % w/w dextran 500,000 (Sigma) in a suspension of 2/1 v/v Dextran/liposome. This mixture was then 12 injected with a syringe into 15 % w/w PEG-8,000 (Sigma) [PEG 8000'(suspension A)] under magnetic stirnng to result in suspension B. The rate of the stirring was SOO-1,000 rpm. A CaCI 2 solution (100 mM) was added to the suspension to reach the final concentration of I mM. 5 Stimng was continued for one hour, then a washing buffer containing I mM CaC 2 and 150 mM NaCl was added to suspension B at the volumetric ratio of 1:1. The suspension was vortexed and centrifuged at 3000 rpm, 2-4 *C, for 30 min. Af:er the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by centrifugation under the same conditions. A schematic of this new process of obtaining 10 cochleates is detailed in Figure 1. The resultant pellet was reconstituted with the same buffer to the desired concentration. Laser light scattering (weight analysis, Coulter N4 Plus) indicates that the mean diameter for the cochleate is 407.2 + 85 run (figure 2b). Example 2. Preparation of empty hydrogel-isolated cochleates from a mixture of dioleoylphosphatidylserine and 1,2-Distearoyl-sn-glycerol-3-phosphoethanolamine-n 15 (poly(ethylene glycol)-5000, DSPE-PEG) precipitated with calcium Step I : Preparation of small unilaniellar vesicles. A solution of dioleoylphosphatidylsenne (DOPS) and 1,2-distearoyl-sn-glycerol- 3 phosphoethanolamine-n-(poly(ethylene glycol)-500 0 ), (DSPE-PEG, Avanti Polar Lipids, Alabaster, AL, USA) in chloroform (ratio of DOPS:DSPS-PEG = 100:1, w:w) was placed in 20 a round-bottom flask and dried to a film using a Buchi rotavapor at 35 *C. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 pim filter. The following steps were carried out in a sterile hood. The dried lipid film was hydrated with de-ionized water to a concentration of 10 mg lipid/ml. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a cooled bath sonicator (Laboratory Supplies Com., Inc.). 13 Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear (suspension A) and there were no liposomes apparently visible under a phase contrast optical microscope with a I0OOX magniFication. Step 2: Preparation of hydrogel isolated cochleates 5 The liposome suspension obtained in step I was then mixed with 40 % w/\v dextran 500,000 in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 (PEG 8000/(suspension A)] under magnetic stirring to result in suspension B. The rate of the stirring was 800-1,000 rpm. A CaC 2 solution (100 mM) was added to the suspension to reach the final concentration of 1 mM. 10 Stirrng was continued for one hour, then a washing buffer containing 1 mM CaCb 2 and 150 mM NaCl was added to suspension B at the volumetric ratio of 1:1. The suspension was \ortexed and centnfuged at 3000 rpm, 2-4 'C, for 30 min. After the supematant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by centnfugation under the same conditions. A schematic of this new process of obtaining 15 cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Phase contrast optical microscopy indicates the formation of uniform, very small, needle-like cochleates. Example 3. Preparation of empty bydrogel-isolated cochleates from a mixture of dioleoylphosphatidylserine and n-octyl-beta-D-gluco-pyranoside precipitated with 20 calcium Step 1 : Preparation of small unilamellar vesicles. A solution of dioleoylphosphatidylserine (DOPS) in chloroform was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at 35 *C. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 pm filter. The following steps were camied 14 out in a stenle hood. The dried hpid film was hydrated with a solution of n-octyl-beta-D gluco-pyranoside (OCG) at I mgml at a ratio of DOPS . OCG of 10.1 w:w. The hydrated suspension was purged and sealed with nitrogen, then sonicated briefly in a cooled bath sonicator. 5 Step 2 : Preparation of hydrogel isolied cochleates The suspension obtained in step I was then mixed with 40 % w/w dextran-500,000 in a suspension of 2/1 v/v Dextran/liposomc. This mixture was then injected via a synnge into 15 % w/w PEG-8,000 [PEG 8000/(suspension A)] under magnetic stirring to result in suspension B. The rate of the stirmng was 800-1,000 rpm. A CaC 2 solution (100 mM) was 0 added to the suspension to reach the final concentration of I mM. Stirrng was continued for one hour, then a washing buffer containing I mM CaC 2 and 150 mM NaCl was added to suspension B at the volumetric ratio of 1:1. The suspension was vortexed and centrifuged at 3000 rpm, 2-4 *C, for 30 min. After the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by 15 centrifugation under the same conditions. A schematic of this new process of obtaining cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Phase contrast optical microscopy indic,!es the formation of uniform, very small, needle-like cochleates. Example 4. Preparation of Amphotericin B-loaded hydrogel-isolated cochleates 20 precipitated with calcium Step I: Preparation of small unilaniellar A mB-loaded, vesicles from dioleoylphosphatidylserine. A mixture of dioleoyl phosphatidylsenne (DOPS) in chloroform (10 mg/ml) and 25 A-mB in methanol (0.5mg/ml) at a molar ratio of 10:1 was placed in a round-bottom flask and 15 dried to a film using a Buchi rotavapor at 40 'C. The rotavapor was s:crilzed by flashing nitrogen gas through a 0.2 pm filter. The following steps were carried out in a sterile hood. The died lipid Film was hydrated with de-ionized water at the concentration of 10 mg lipid/ml. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a 5 cooled bath sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear yellow (suspension A) and there were no liposomes apparently visible under a phase contrast microscope with a IOOOX magnification. Step 2 : Preparation of AmB-loaded, hydrogel- isolated cochleates 10 The liposome suspension obtained in step I was then mixed with 40 % w/w dextran 500,000 in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 (PEG 8000/(suspension A)] under magnetic stirring to result in suspension B. The rate of the stirring was 800-1,000 rpm. A CaC 2 solution (100 mM) was added to the suspension to reach the final concentration of ImM. 15 Stirrng was continued for one hour, then a washing buffer containing 1 mM CaC 2 and 150 m.M NaCI was added to suspension B at the volumetric ratio of 1:1. The suspension was vortexed and centnfuged at 3000 rpm, 2-4 *C, for 30 min. Afler the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by centrifugation under the same conditions. A schematic of this new process of obtaining 20 cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Laser light scattering (weight analysis, Coulter N4 Plus) indicate that the AjmB-cochleates mean diameter was 407.3 -_- 233.8 nm (figure 2a). 16 Example 5. Preparation of Doxorubicin (DXR) loaded hydrogel isolated cochleates precipitated witi calcium Step 1 Preparation of sIall unilante/lar DXR-loaded vesiclesfrom dioleoylphosphatidylserfinLe. 5 A mixture of dioleovlphosphatidylsenine (DOPS) in chloroform (10 mg/ml) and (DXR) in methanol (0.5mg/mil) at a molar ratio of 10:1 was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at room temperature. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 pm filter. The following steps were cared 10 out in a stenle hood. The dried lipid Film was hydrated with de-ionized water at the concentration of 25 mg lipid/ml. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a cooled bath sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear pink (suspension A) and there were no liposomes apparently visible under 15 phase contrast microscope with a I0OOX magnification. Step 2 : Preparation of DXR-loaded. hydrogel isolated cochleates 5 ml of the liposome suspension obtained in step I was then mixed with 40 % w/w dextran-500,000 (Sigma) in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a syringe into IS % w/w PEG-8,000 [PEG 8000/(suspension A)] under 20 magnetic stiming to result in suspension B. The rate of the stirring was 800-1,000 rpm. A CaCl 2 solution (100 mM) was added to the suspension to reach the final concentration of ImM. Stirnng was continued for one hour, then a washing buffer containing I mM CaC 2 and 150 mM NaCl was added to suspension B at the volumetric ratio of 1:1. The suspension 25 was vortexed and centrifuged at 6400 rpm, 2-4 "C, for 30 min. A schematic of this new 17 process of obtaining cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Laser light scattering (weight analysis, Coulter N4 Plus) confirmed the formation of small DXR-cochleates. Example 6. Preparation of Cyclosporin A (CSPA) -loaded hydrogel isolated cochleates 5 precipitated with calcium Step I : Preparation of small unilamellar CSPA-loaded vesiclesfrom dioleoylphosphatidylserine. A mixture of dioleoylphosphaidylserine (DOPS) in chloroform (10 mg/ml) and 10 CSPA in methanol (0.5mg/ml) at a molar ratio of 10:1 was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at room temperature. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 .im filter. The following steps were camed out in a stenle hood. The dried lipid film was hydrated with de-ionized water at the concentration of 10 mg lipid/ml. The hydrated suspension was purged and sealed with 15 nitrogen, then sonicated in a cooled bath sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear (suspension A) and there were no liposomes apparently visible under a phase contrast microscope with a 1000X magnification. Step 2 : Preparation of CSPA-loaded, hydrogel isolated, cochleates 20 The liposome suspension obtained in step I was then mixed with 40 % w/w dextran 500,000 in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 (Sigma) [PEG 8000/(suspension A)] under magnetic stirnng to result in suspension B. The rate of the stiming was 800-1,000 rpm. A CaC 2 solution (100 mM) was added to the suspension to reach the final concentration o f 1 mM. 18 Stirring was continued for one hour, then a washing buffer containing I mM CaC1 2 and 150 mM NaCl was added to suspension B at the volumetric ratio of I 1. The suspension was vortexed and centrifuged at 3000 rpm, 2-4 CC, for 30 min. After the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5.1, followed by 5 centrifugation under the same conditions. A schematic of this new process of obtaining cochlcates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Laser light scattering (weight analysis, Coulter N4 Plus) confirmed the formation of small CSPA-cochleates. Example 7. Preparation of Nelfinavir (NVIR)loaded hydrogel isolated cochleates 0 precipitated with calcium Siep I : Preparaiion of small undamellar NVIR-loaded vesicles from dioleoylphosphatidylserine A mixture of dioleoylphosphatidylsennc (DOPS) in chloroform (10 mg/ml) and 5 NVVR in methanol (0.5mg/ml) at a molar ratio of 10:1 was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at RT. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 prm filter. The following steps were cared out in a sterile hood. The dried lipid film was hydrated with de-ionized water at the concentration of 10 mg lipid/il. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a 20 cooled bath sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear (suspension A) and there were no liposomes apparently visible under a phase contrast microscope with a I OOOX magnification. 19 Step 2 : Preparation of NVIR-loaded hydrogel isolated, cochleates The liposome suspension obtained in step I was then mixed with 40 % w/w dextran 500,000 in a suspension of 2/1 v/v Dextran/iposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 (PEG 8000/(suspension A)) under magnetic stirring to 5 result in suspension B. The rate of the stirring was 800-1,000 rpm. A CaCI 2 solution (100 mM) was added to the suspension to reach the final concentration of ImM. Stirring was continued for one hour. then a washing buffer containing 1 mM CaC 2 and 150 mM NaCl was added to suspension B at the volumetric ratio of 1:1. The suspension was vortexed and centrifuged at 3000 rpm, 2-4 *C, for 30 min. After the supernatant was 10 removed, additional washing buffer was added at the volumetric ratio of 0.5.1, followed by centrifugation under the same conditions. A schematic of this new process of obtaining cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Laser light scattering (weight analysis, Coulter N4 Plus) confirmed the formation of small NVIR-cochleates. 15 Example 8. Preparation of Rifampin (RIF) -loaded hydrogel-isolated cochleates precipitated with calcium Step I : Preparation of small unilaniellar RIF-loaded vesicles from dioleoylphosphatidylserine. 20 A mixture of dioleoylphosphatidylserine (DOPS) in chloroform (10 mg/mI) and RIF in methanol (0.5mg/ml) at a molar ratio of 10:1 was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at RT. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 pm filter. The following steps were carried out in a sterile hood. The dried lipid film was hydrated with de-ionized water at the concentration of 10 mg lipid/ml. The 25 hydrated suspension was purged and sealed with nitrogen, then sonicated in a cooled bath 20 sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear (suspension A) and there were no liposomes apparently visible under a phase contrast microscope with a I 0OOX magnification. Step 2 Preparation of RJF-loaded. hydrogel isolaze.d cochleates 5 The liposome suspension obtained in step 1 was then mixed with 40 % w/w dextran 500,000 (Sigma) in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 (Sigma) [PEG 8000/(suspension A)] under magnetic stirnng to result in suspension B. The rate of the stirring was 800-1.000 rpm. A CaCl 2 solution (100 mlM) was added to the suspension to reach the final concentration of 10 1mM. Stirring was continued for one hour, then a washing buffer containing I rmN CaCl and 150 mM NaCl was added to suspension B at the volumetric ratio of 1:1. The suspension was vortexed and centrifuged at 3000 rpm, 2-4 C, for 30 min. After the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by 15 centrifugation under the same conditions. A schematic of this new process of obtaining cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Laser light scattering (weight analysis, Coulter N4 Plus) confirmed the formation of small RIF-cochleates. 21 Example 9. Preparation of Vitamin A loaded hydrogel isolated cocbleates precipitated with calcium Step I : Preparation of small unilamellar Vitamnin A -loaded vesicles from 5 dioleoylphosphaidylserine. Vitamin A (retinol) is sensitive to air oxidation and is inactivated by UV light. Vitamin A is protected when embedded into lipid bilayers. The incorporation is achieved as follows : 10 A mixture of dioleoylphosphatidylserine (DOPS) in chloroform (10 mg/ml) and Vitamin A in methanol (0.5mg/ml) at a molar ratio of 10:1 was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at RT. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 im filter. The following steps were cared out in a sterile hood. The dried lipid film was hydrated with de-ionized water at the concentration of 10 nig 15 lipid/ml. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a cooled bath sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear (suspension A) and there were no liposomes apparently visible under a phase contrast microscope with a OOOX magnification. 20 Step 2 : Preparation of Vitamin A-loaded, hydrogel isolated, cochleates The liposome suspension obtained in step I was then mixed with 40 % w/w dextran 500,000 in a suspension of 2/1 v/v DextranA/liposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 [PEG 8000/(suspension A)] under magnetic stirring to result in suspension B. The rate of the stirring was 800-1,000 rpm. A CaC 2 solution (100 25 mM) was added to the suspension to reach the final concentration of 1mM. 22 Stirring was continued for one hour, then a washing buffer containing I mnM CaC and 150 mM NaCl was added to suspension B at the volumetric ratio of II. The suspension was vortexed and centrifuged at 3000 rpm, 2-4 'C, for 30 min. After the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by 5 centrifugation under the same conditions. A schematic of this new process of obtaining cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Example 10. Preparation of polyunsaturated fatty acid (PFA) loaded hydrogel isolated cochleates precipitated with calcium 10 PFA's are biologically relevant molecules involved in the control of the level of cholesterol in blood and are the precursors of prostaglandins. PFA's are sensitive to oxidation which limits their incorporation in food. PFA's undergo, in the presence of oxygen, a series of reactions called autoxidation, leading to aldehydes then ketones which has a fishy 15 unpleasaant odor and flavor. Embedding PFA in rigid, enrolled, lipid bilayers help preventing the autoxidation cascade. A general method of preparing PFA- cochleates is as follows Step I . Preparation of/small unlamellar PFA -loaded vesicles from dioleoylphosphaidylserine. A mixture of dioleoylphosphatidyisenine in chloroform (10 mg/ml) and PFA in 20 methanol (0.5mg/ml) at a molar ratio of 10:1 was placed in a round-bottom flask and dried to a film using a rotary evaporator at RT. The rotary evaporator was sterilized by flashing nitrogen gas through a 0.2 pim filter. The following steps were cared out in a sterile hood. The dried lipid film was hydrated with de-ionized water at the concentration of 10 mg lipid/ml. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a 25 cooled bath sonicator. Sonication was continued (for several seconds to several minutes 23 depending on lipid quantity and nature) until the suspension became clear (suspension A) and there were no liposomes apparently visible under a phase contrast microscope with a I 0OOX magnification. Step 2 : Preparation of PFA-loaded, hydrogel isolated. cochleates 5 The liposome suspension obtained in step 1 was then mixed with 40 % w/w dextran 500,000 in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a synnge into 15 % w/w PEG-8,000 [PEG 8000/(suspension A)] under magnetic stirnng to result in suspension B. The rate of the stirnng was 800-1,000 rpm. A CaC 2 solution (100 mM) was added to the suspension to reach the final concentration of ImM. 0 Stirrng was continued for one hour, then a washing buffer containing I mM CaC 2 and 150 mnM NaCl vas added to suspension B at the volumetnc ratio of 1:1. The suspension was vortexed and centnfuged at 3000 rpm, 2-4 'C, for 30 min. After the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by centrifugation under the same conditions. A schematic of this new process of obtaining 15 cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. Example 11. Preparation of Cinnamon Oil (CinO) loaded hydrogel isolated cochleates precipitated with calcium Step I :-Preparation of small unilamellar CinO-loaded vesicles from 20 dioleoylphiosphatidylserine. A mixture of dioleoylphosphatidyl serine (DOPS) in chloroform (10 rng/ml) and CinO in methanol (0.5mg/ml) at a molar ratio of 10:1 was placed in a round-bottorn flask and dried to a film using a Buchi rotavapor at 40 *C. The rotavapor was sterilized by flashing 25 nitrogen gas through a 0.2 pm filter. The following steps were carried out in a sterile hood. 24 The dried lipid film was hydrated wa th de-ionized water at the concentration of 10 mg lhpid/ml. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a cooled bath sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear (suspension A) and 5 there were no liposomes apparently visible under a phase contrast microscope with a 1OOX magnification. Step 2 Preparaion of CinO-loaded, hydrogel isolated, cochleates The liposome suspension obtained in step I was then mixed with 40 % w/w dextran 500,000 in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via 3 0 syringe into 15 % w/w PEG-8,000 [PEG 8000/(suspension A)) under magnetic sting to result in suspension B. The rate of the stirring was 800-1,000 rpm. A CaCl: solution (100 mM) was added to the suspension to reach the final concentration of In.M. Stirring was continued for one hour, then a washing buffer containing 1 mN CaCb2 and 150 mM NaCl was added to suspension B at the volumetric ratio of 1:1. The suspension 15 was vortexed and centnfuged at 3000 rpm, 2-4 'C, for 30 min. After the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by centrifugation under the same conditions. A schematic of this new process of obtaining cochleates is detailed in Figure 1. The resulting pellet was reconstituted with the same buffer to the desired concentration. 25 Example 12. Preparation of DNA loaded hydrogel isolated cochleates precipitated with calcium Step I Preparation of small unilamellar DN A-loaded vesiclesfrom 5 dioleoylphosphatidylserine A solution of dioleoylphosphatidylsenine in chloroform (10 mg/ml) was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at RT. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 pm filter. The following steps were cared 10 out in a sterile hood. The dried lipid film was hydrated with a solution of pCMV-beta-gal DNA in TE buffer (at I mg/ml) to reach a concentration of DOPS DNA of 10.1 and a concentration of 10 mg lipid/ml. The hydrated suspension was purged and sealed with nitrogen, then vortexed for several minutes. Step 2 : Preparation of DNA-loaded, hydrogel isolated, cochleates 15 The DNAiliposome mixture was then mixed with 40 % w/w dextran-500,000 in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 [PEG 8000/(suspension A)] under magnetic stirring to result in suspension B. The rate of the stirring was 800-1,000 rpm. A CaC1 2 solution (100 mM) was added to the suspension to reach the final concentration of ImM. 20 Stirring was continued for one hour, then a washing buffer containing I mM CaCl 2 and 150 mM NaCI was added to suspension B at the volumetric ratio of 10:1. The suspension was vortexed and centrifuged at 3000 rpm, 2-4 *C, for 30 min. After the supermatant was removed, additional washing buffer was added at the volumetric ratio of 5:1, followed by centrifugation under the same conditions. A schematic of this new process of 26 obtaining cochleates is detailed in Figure I The resulting pellet was reconstituted with the same buffer to the desired concentration. Example 13. Preparation of empty hydrogel isolated-cochleates precipitated with zi[c Step I . Preparation of small unilamel!ar vesicles from dioleoylphospharidylserie. 5 A solution of dioleoylphosphatidylsenne (DOPS) in chloroform (10 mg/ml) was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at 35 *C. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 pm filter. The following steps were carried out in a sterile hood. The dried lipid film was hydrated with de-ionized water at the concentration of 10 mg lipid/ml. The hydrated suspension was purged and sealed with 0 nitrogen, then sonicated in a cooled bath sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear (suspension A) and there were no liposomes apparently visible under a phase contrast microscope with a I0OOX magnification. Step 2 : Preparation of hydrogel isolated cochleates 15 The liposome suspension obtained in step I was then mixed with 40 % w/w dextran 500,000 in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 [PEG 8000/(suspension A)] under magnetic stirring to result in suspension B. The rate of the stirring was 800-1,000 rpm. A ZnC 2 solution (100 mM) was added to the suspension to reach the final concentration of 1 mM. 20 Stirring was continued for one hour, then a washing buffer containing 1 ImM ZnC12 and 150 mM NaCl was added to suspension B at the volumetric ratio of 1:1. The suspension was vortexed and centnfuged at 3000 rpm, 2-4 1C, for 30 min. After the supernatant was 27 removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by centrifugation under the same conditions A schematic of this new process of obtaining cochleates is detailed in Figure 1 . The resulting pellet was reconstituted with the same buffer to the desired concentration. Laser light scattering (weight analysis, Coulter N4 Plus) 5 confirmed the formation of small cochleates. Example 14. Preparation of Amphotericin B-loaded hydrogel-isolated cochleates precipitated with zinc Step I : Preparation of small unilamellar A mB-loaded vesiclesfrom dioieoylphospiatidy.lserin e. 10 A mixture of dioleoyl phosphatidylserine (DOPS) in chloroform (10 mg/ml) and AmB in methanol (0.5 mg/ml) at a molar ratio of 10:1 was placed in a round-bottom flask and dried to a film using a Buchi rotavapor at 40 *C. The rotavapor was sterilized by flashing nitrogen gas through a 0.2 pm filter. The following steps were carried out in a stenle hood. 5 The dried lipid film was hydrated with de-ionized water at the concentration of 10 mg lipid/ml. The hydrated suspension was purged and sealed with nitrogen, then sonicated in a cooled bath sonicator. Sonication was continued (for several seconds to several minutes depending on lipid quantity and nature) until the suspension became clear yellow (suspension A) and there were no liposomes apparently visible under a phase contrast microscope with a 20 1000X magnification. Step 2 : Preparation of A mB-loaded hydrogel isolated cochleates The liposome suspension obtained in step I was then mixed with 40 % w/w dextran 500,000 in a suspension of 2/1 v/v Dextran/liposome. This mixture was then injected via a syringe into 15 % w/w PEG-8,000 [PEG 8000/(suspension A)] under magnetic stirring to 28 result in suspension B The rate of the stirring was 800-1,000 rpm. A ZnCIb solution (100 mM) was added to suspension to reach the final concentration of 1 mM. Stirring was continued for one hour, then a washing buffer containing 1 mM ZnC 2 and 150 mM NaCI was added to the suspension B at the volumetric ratio of 1:L. The 5 suspension was vortexed and centnfuged at 3000 rpm, 2-4 *C, for 30 min. After the supernatant was removed, additional washing buffer was added at the volumetric ratio of 0.5:1, followed by centifugation under the same conditions. A schematic of this new process of obtaining cochleates is detailed in Figure I. The resultant pellet was reconstituted with the same buffer to the desired concentration Laser light scattering (weight analysis, Coulter N4 10 Plus) confirmed the formation of small AmB-Zn-cochleates. Example 15. Microscopic observation of hydrogel-isolated cochleates Optical microscopic study was performed stepwise alone with the preparation procedure in order to gain some mechanistic details of the hydrogel-isolated cochleates formation. 15 The microscopic images seen in Figures 3a,b and 4a-f show the morphological changes at each preparation step of AmnB loaded hydrogel isolated-cochleates precipitated with Ca 2 ions. When the AmB/liposome-dextran mixture was dispersed into PEG solution, phase separation resulted as shown by Fig. 3a. Partition of the liposomes favored the dispersed dextran phase as indicated by a yellow color of AmB. This panitioning ensures 20 that liposomes are isolated in each dextran particles. Addition of Calcium ions into the continued phase (PEG) resulted in formation of precipitates in the dispersed phase. As the final product, small needle-shape cochleates were formed and observed under the microscope, these cochleates opened into unilamellar vesicles upon addition of EDTA and 29 chelation of the calcium (Figures 4a,b). The needle-shaped morphology was confirmed by scanning electron microscopy after freeze-fracture (Figure 5). Similar microscopic images were obtained for empty and AmB-Zi-precipitated hydrogel isolated-cochleates (Figures 4c,d) and empty Zn-precipitated hydrogel isolated-cochleate (Figures 4e,f) Example 16. Antifungal Activity of hydrogel isolated cochleates loaded with Amphotericin B, ii vitro Growth Inhibition of Candida albicans An in viaro yeast susceptibility assay was performed companng the inhibitory and lethal effects of AmB-cochleates, AmBisomes liposomall formulation of AmB) and 0 AmB/DMSO. Five colonies of freshly growing Candida albicans were selected from a YPD agar plate (from a 48 hour culture) and added to 2 ml of 2x YPD broth, pH 5.7. The ODgo of this stock culture was measured and the yeast density was adjusted to OD 590 = 0.1 and 0.1ml of this suspension added to each well of a 96 well plate. AmB/cochleates, AmB/DMSO and kmBisomes were added to 96 well plates to a .5 final concentration of 0.078, 0.156, 0.3125, 0.625, 1.25 and 2.5 pg/ml of AmB. The 96 well plates were incubated at 37*C with gentle shaking and cell density was measured on a 96 well plate reader (Molecular Devices Spectramax 340) at 0, 2, 4, 6, 24 and 30 hours. Figure 6 shows that AmB-cochleates have a greater growth inhibitory effect than AmBisomes liposomall formulation of AmB). 20 Fungicidal effect of hydrogen isolated cochleates loaded with Amphotericin B. Aliquots of yeast cells (50 pl) were removed from the 96 well plates and serially diluted (up to 1:10000 for plating onto agar plates) and counted using a hemocytometer. Fifty pl of the diluted yeast cells were plated onto YPD agar plates and incubated for 24 30 hours at 37C. Yeast colonies were counted using a BioRad Fluor-S Multi-lmager equipped with Quanitity One"' software. Yeast cells treated with AmBisonie, AmB/DMSO and AmB/cochleates (0.625 ug AmB/ml) were examined for the ratio of colony forming units to total cell number after 30 5 hours of incubation. The results show that the AmB/cochleates had the greatest lethal effect on the yeast cells compared to the other antifungal agents tested. There was nearly 0% yeast viability after treatment with the AmB-cochleates and 12% yeast viability after trea:nent with AmrB/DMSO. The AmBisome was not as effective, resulting in 52% yeast viability (Figure 7). 0 Macrophage Protection with AmB cochleates Particle scavenging cells, such as macrophage, are the first line of defense against many microbial infections. However, many microbes, which induce severe human clinical infections, have been shown to infect macrophage and avoid destruction. It is possible that in vivo, macrophage play an important role in the uptake of .5 cochleates, via an endocytotic mechanism. Since macrophage also play an important role in the host defense and clearance of fungi and parasites, it is important to study the interaction between macrophage and cochleates. The following examples indicate that the cochleates are taken up by macrophage. Large doses of AmiB delivered to the macrophage were found to be non-toxic and remained 0 within the macrophage in a biologically active form. AmB cochleates provided protection for the macrophage against infection by Candida albicans when administered prior to or after fungal infection. 31 Prop hylactic dose regime J774A. I macrophage (NI) were subcultured into a 96-well plate at a concentration of Ix 105 cells/ml in DNEN +10% FBS. I100pi ArnB cochleates (AmBc 0.2, 0.6, 1.25, and 2.5 pg AniB/m!), Funuizoiie, or empty cochleates (EC at 2, 6, 12.5, and 25pg lipid/ml) were added at the specified concentration. Plates were incubated overnight at 37oC 5 and 5% Co 2 . 24 hours later, the medium was replaced. This step was performed twice. Candida albicans (CA) was added to the plate at a concentration of 2.5x 103 cells/ml, a ratio of 1:200 with respect to the macrophage Plates were incubated overnight under the conditions stated above. Following the 24 hr incubation, the plates were removed and observed. Medium was ) pipetted vigorously to remove and disrupt the cells, 2 5 pl of this suspension was placed onto Sabouraud Dextrose Agar plates, and then placed in a dry incubator ovemight at 37*C. C. albicans CFU's were counted the following day. The data in Figure 8 suggest that Am-rB cochleate loaded macrophage are very effective at killing the fungal cells. Post-infection dose regime: J774A.1 macrophage (M) were subcultured into a 96-well plate i then incubated overnight. Following incubation, the macrophage were infected with CA at a ratio of 200.1, then subsequently AmrrBc, Fungizone or EC was added at the specified concentrations. Twenty four hours later, the cell cultures were observed and CFU's determined as described above. When M were challenged with CA and subsequently dosed with AnB cochleates, the 0 CFU count was again nearly zero. These results indicate that macrophage engulf and concentrate AmB cochleate, as macrophage were protected against C. albicans challenge after AmB cochleate had been washed off (Figure 8). 32 In contrast, Fungizonc, (AmB in deoxycholate), the most popular clinical form of A.mB was extremely toxic' and lethal to the macrophage in viiro. Within 5 hours of administration, there was a large amount of cellular debris found in the petri dish, with no signs of viable macrophage. 5 Microscopic observation reveals the AmB cochleates are not toxic to the macrophage even at the highest doses studied. The AmB cochleates are accumulated at high levels resulting in large distended vacuoles. After washing of the macrophage and incubating again for 24 hr, most of the vacuoles had returned to the normal shape and size, yet a few were noticeably enlarged. A few macrophage were even noticed to be "moving" with the enlarged 10 vacuoles. A-mB cochleates are concentrated within the vacuoles and it is probable that AmB is released gradually over time. Example 17 : Evaluation of tissue penetration of AmB after iv administration of amphotericin b bydrogel isolated cochleates Tissue penetration of amphotenicin B has been evaluated after iv administration. 15 Groups (n=5) of C57BL/6 mice (20 -23 g) were given iv (0.625 mg/kg) AmB cochleates (0.05 ml/ 20 g) with a K cc U 100 insulin syringe with a 18 g K needle size. At predetermined sacrifice times (2, 5, 10, 20 and 40 min, 1, 2, 3, 4, 6, 8, 12, 24, 36 and 48 his), animals were giving anesthesia, blood collected via cardiac puncture, then euthanized, dissected and tissues of interest removed (brain, lung, liver, spleen, kidneys, heart, fat, 20 stomach, stomach contents, intestine and intestine contents) and weighed. For analysis of AmB, samples were mixed with extraction solvent (10% methanol, 35% water, 55/o ethanol), homogenized, sonicated and centrifuged. A 90 l aliquot of supernatant was transfered into a micro vial, injected into the HPLC system in a Nova-Pak C-18 column (3.9xl50 mm, 4 pm particle size) kept at 40 *C. Amphotenicin B was eluted at a flow rate of 0.5 ml/min with 29% 33 methanol, 30% acetonitril: and 41 % 2 5 mM EDTA and detected at 40S rim. The concentration of AmB was calculated \ ith the help of an external standard curve. In Figure 9 the tissue exposure after a single iv dose of AmB cochleates is shown. Large penetration in key tissues like liver, spleen and kidney can be obser-ved. 5 Example 18 : Oral delivery of AmB mediated by hydrogel isolated cochleates loaded wvith AmB. Single dose regime Oral availability of the hydrogel isolated cochleates loaded with AmB has been examined by intragastnc administration of the formulation of example 2 to overnight fasting, 10 C57BL16 mice (20-23g). One tenth ml of the formulation at the dose of 10 mg/kg was administrated to 9 mice. Three mice from each group were sacrificed at 1, 6 and 24 hrs post administration followed by analysis of AmB level in organs and tissues. Tissue and blood samples were processed as follows: tissues were diluted 1/20 or 1/10 by addition of extraction solvent (H 2 0 35%, methanol 10%, ethanol 55% w/w/w nv/v/v) and 15 homogenized with an Ultra-Turrex@ device. A 0.5 ml aliquot was taken, sonicated for 1 min and centrifuged at 7260 rpm for 12 min at 4*C. Supernatant was transferred to an HPLC micro-vial and 30 pIl injected on a C-18, 3.9 x 150 mm, 4 ym particle sized analytical column with a flow rate of 0.5 ml, at 40*C. Concentration of Am.B detected at 408 nm was calculated with the help of an external calibration curve. 20 Figure 10 shows the time profile of AmB in the tissues over a period f time of 24 hrs. Although only three time points are plotted, accumulation in key tissues (liver, lungs, spleen and kidneys) can be seen. 34 Multiple dose regime Two other groups of mice received a 10 mg/kg/day oral multiple dose regime for ten days and one group was sacrificed 24 hrs after the last dose and the other group 20 days after the last dose received. At the predetermined time points mice were anesthetized, sacrificed 5 and dissected for tissue collection. Tissucs were processed as in single dose regime and AmB level determined by HPLC. Results from 24 h after the 10'h dose are depicted in Figure 1 and show that hydrogel isolated cochleates allow the delivery of AmB from the gastrointestinal tract at therapeutic levels. Example 19 : Correlation between biodistribution in healthy and infected mice and the 10 level of Candida albicans in tissue after oral administration Figure 12 shows the relationship between tissue levels of amphotericin B (pg/g tissue on left scale) and efficacy as decrease of Candida albicans infection (CFU/g on the night scale) after oral administration of AmB-cochleates (Figure 12). After oral administration of 10 mg/kg/day for 10 consecutive days to healthy mice, 15 AnB presented high levels in kidneys followed by lungs, spleen, liver and brain, which shows much lower levels than the other tissues. It has been shown that disease state affects pharmacokinctics of drugs at different levels. This phenomenon can be seen clearly in the graph: AmB in tissue reaches lower levels in C. albicans infected mice after oral administration of 10 mg/kg/day (same dose) for 15 days, 5 more doses than the healthy 20 group. It also shows a change in the distribution pattern where the lungs are the target tissue with lowest levels. Oral administration of an AmB cochleate formulation at 10 mg/kg/day for 15 days provided high efficacy. The decrease in CFU/g in kidney tissue is about 3.5 logs for the cochleate formulation. In lungs, AmB cochleate formulations completely eradicate C. 35 P \OPER\Ejh\Ejhcsponses\243863 biodeliverydoc-2N02/2007 albicans and clears the lungs of fungal infection. It is clear that the cochleate delivery system provides a high level of AmB in infected animals, this correlates with the higher efficiency seen in the cochleate formulation, indicating that AmB-cochleates are a suitable vehicle for oral treatment of systemic Candidiasis. 5 In addition, orally administered AmB-cochleates were non-toxic even at the highes dose of 50 mg/kg (no lesions were found in kidneys, GI tract and other organs of mice given 10, 20 and 50 mg/kg of AmB-cochleates). This high does (50 mg/kg) is equivalent to 100 times the lowest does (0.5 mg/kg) that showed 100% of survival in the Candida 10 infected mouse model. Throughout this specification, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or integer or group of elements or integers but not 15 the exclusion of any other element or integer or group of elements or integers. Reference to any prior art in this specification is not, and should not be taken as, an acknowledgment or any form of suggestion that that prior art forms part of the common general knowledge in any country. 20 36

Claims (34)

1. A method for producing lipid-based cochleates comprising the steps of: a) providing an aqueous suspension of liposomes; b) mixing the liposome suspension with polymer A; c) adding the liposome/polymer A suspension into a solution comprising polymer B, wherein polymer A and polymer B are immiscible, thereby creating a two phase polymer system; d) adding a solution of a cationic moiety to the two-phase polymer system; and e) washing the two-phase polymer system to remove the polymers.

2. A method of Claim 1 wherein the liposomes are formed of lipid comprising negatively charged lipid.

3. A method of Claim 2 wherein the negatively charged lipid is phosphatidylserine.

4. A method according to any one of Claims 1 to 3 wherein the size of the cochleates is less than 1 micron.

5. A method for producing lipid-based cochleates comprising the steps of: a) providing an aqueous suspensions containing a detergent-lipid mixture; b) mixing the detergent-lipid suspension with polymer A; c) adding the detergent-lipid/polymer A suspension into a solution comprising polymer B, wherein polymer A and polymer B are immiscible, thereby creating a two phase polymer system; d) adding a solution of a cationic moiety to the two-phase polymer system; and e) washing the two-phase polymer system to remove the polymer.

6. A method for producing lipid-based cochleates according to Claim 5 wherein the cochleate comprises a biologically active molecule. - 37 - C:\NRPorbl\DCC\AAR\2935009 1.DOC-5/05/20I10

7. A method according to Claim 6 in which the biologically relevant molecule is selected from the group consisting of drugs, polynucleotides, polypeptides and antigens.

8. A method of Claim 7 wherein the biologically relevant molecule is a drug selected from the group consisting of antiviral agents, anaesthetics, anti-infectious agents, antibacterial agents, antifungal agents, anticancer agents, immunosuppressants, steroidal anti-inflammatory agents, non-steroidal anti-inflammatory agents, tranquilisers and vasodilators.

9. A method of Claim 8 in which the drug is at lest one member selected from the group consisting of Amphotericin B, acyclovir, Adriamycin, cabamazepine, melphalan, nelfinavir, nifedipine, indomethacin, naproxen, estrogens, testosterones, steroids, phenytoin, ergotamines, cannabinoids, rapamycin, propanidid, propofol, alphadione, echinomycine, miconazole nitrate, teniposide, taxol, taxotere and rifampin.

10. A cochleate composition comprising: a) a negatively charged lipid; b) a cation component; and c) a biologically relevant molecule other than a) and b) wherein the cochleates have a mean particle size less than 1 pm, and wherein said cation component is a di- or higher-valent metal ion.

11. A cochleate composition according to Claim 10 in which the biologically relevant molecule is selected from vitamins, minerals, amino acids, toxins, microbicides, microbistats, co-factors, enzymes, polypeptides, polypeptide aggregates, polynucleotides, lipids, carbohydrates, nucleotides, starches, pigments, fatty acids, hormones, cytokines, viruses, organelles, steroids and other multi-ring structures, saccharides, metals, metabolic poisons and drugs. - 38 - C:\NRPortb\DCC\AAR\2935009 1DOC-5/05/2010

12. A cochleate composition according to Claim 11 wherein the biologically relevant molecule is a drug selected from the group consisting of antiviral agents, anaesthetics, anti infectious agents, antibacterial agents, antifungal agents, anticancer agents, immunosuppressants, steroidal anti-inflammatory agents, non-steroidal anti-inflammatory agents, tranquilisers and vasodilators.

13. A cochleate composition according to Claim 12 wherein the drug is at least one member selected from the group consisting of Amphotericin B, acyclovir, Adriamycin, cabamazepine, melphalan, nelfinavir, nifedipine, indomethacin, naproxen, estrogens, testosterones, steroids, phenytoin, ergotamines, cannabinoids, rapamycin, propanidid, propofol, alphadione, echinomycine, miconazole nitrate, teniposide, taxol, taxotere and rifampin.

14. A cochleate composition according to Claim 10 in which the biologically relevant molecule is Amphotericin B.

15. A cochleate composition according to Claim 10 in which the biologically relevant molecule is naproxen.

16. A cochleate composition according to Claim 10 in which the biologically relevant molecule is a polypeptide selected from cyclosporine, angiotension I, II and III, enkephalins and their analogs, ACTH, anti-inflammatory peptides I, II, III, bradykinin, calcitonin, b-endorphin, dinorphin, leucokinin, leutinizing hormone releasing hormone (LHRH), insulin, neurokinins, somatostatin, substance P, thyroid releasing hormone (TRH) and vasopressin.

17. A cochleate composition according to Claim 10 in which the biologically relevant molecule is an antigen.

18. A cochleate composition according to Claim 17 wherein the antigen is a carbohydrate, a polynucleotide or a protein. - 39 - C:NRPortbI\DCC\AAR\2935009 I.DOC-5/05/2010

19. A cochleate composition according to Claim 18 wherein the protein antigen is selected from envelope glycoproteins from influenza or Sendai viruses, animal cell membrane proteins, plant cell membrane proteins, bacterial membrane proteins and parasitic membrane protein.

20. A cochleate composition according to Claim 10 in which the biologically relevant molecule is vitamin A.

21. A cochleate composition according to Claim 10 in which the biologically relevant molecule is polyunsaturated fatty acid.

22. A cochleate composition according to Claim 10 in which the biologically relevant molecule is polynucleotide.

23. A cochleate composition according to Claim 22 wherein the polynucleotide is DNA.

24. A cochleate composition according to Claim 10 in which the metal ion is selected from calcium, magnesium, zinc and barium.

25. A cochleate composition according to Claim 10 or 11 in which the negatively charged lipid is phosphatidylserine.

26. Cochleate composition according to any of Claims 10 to 25, or the product of a method of any Claims 1 to 26, for use in a method of delivering a biologically relevant molecule to a host. - 40 - C:\NRPotbI\DCC\AAR\2 93 5009 I.DOC-5/05/20 10

27. Use of cochleate composition according to any of Claims 10 to 25, or of the product of a method according to Claims 1 to 26, in the manufacture of a composition for use in a method of treatment of the human or animal body or to deliver a biologically relevant molecule to a host.

28. Use according to Claim 27 in which the composition is administered by a mucosal or a systemic route.

29. Use of Claim 28 wherein the composition is administered by a mucosal route selected from oral, intranasal, intraocular, intraanal, intravaginal, parenteral or intrapulmonary.

30. Use according to Claim 29 in which the route is oral.

31. Use according to Claim 29 in which the composition is an aerosol.

32. Use according to Claim 28 wherein the composition is administered by a systemic route selected from intravenous, intramuscular, subcutaneous, transdermal or intradermal.

33. A pharmaceutical composition comprising a cochleate composition according to any of Claims 10 to 25 and a pharmaceutically acceptable carrier.

34. A method according to any one of Claims 1 to 9 or a composition of Claims 10 to 26 or 33 or a use of Claims 27 to 32 substantially as herein described with reference to the Figures and/or Examples. -41 -