AU2007264560B2

AU2007264560B2 - Bio-degradable/absorbable polymer having reduced metal catalyst content, and process for production thereof

Info

Publication number: AU2007264560B2
Application number: AU2007264560A
Authority: AU
Inventors: Hidetoshi Arimura; Yoshitake Takahashi; Koji Yamauchi
Original assignee: Gunze Ltd
Current assignee: Gunze Ltd
Priority date: 2006-06-28
Filing date: 2007-06-18
Publication date: 2011-12-01
Anticipated expiration: 2027-06-18
Also published as: EP2039714A4; CA2653295A1; CA2653295C; NZ573125A; AU2007264560A1; KR101102475B1; WO2008001633A1; US20130066042A1; US8481673B2; TWI440651B; IN2014DN10439A; HK1129123A1; EP2039714B1; EP2039714A1; KR20090023599A; BRPI0713060A2; TW200806704A; US20090171064A1

Abstract

Disclosed are: a safe bio-degradable/absorbable polymer having an extremely low metal catalyst content while retaining properties desired for a medical implant; a process for the production of the polymer; and a method for reducing the content of a metal catalyst in a bio-degradable/absorbable polymer, which can be applied on an industrial scale. Specifically, disclosed is a process for the production of a bio-degradable/absorbable polymer having a content of a metal catalyst of less than 1 ppm in terms of a metal, comprising the following steps (1) and (2): (1) copolymerizing lactide and ε-caprolactone at a molar ratio ranging from 40/60 to 60/40 in the presence of a metal catalyst to produce a copolymer; and (2) washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a ratio ranging from 25/75 to 45/55 by volume at a temperature lower than 40˚C, and then drying the copolymer. Also specifically disclosed is a process for the production of a bio-degradable/absorbable polymer having a content of a metal catalyst of less than 1 ppm in terms of a metal, comprising the following steps (1) and (2): (1) copolymerizing lactide and ε-caprolactone at a molar ratio ranging from 65/35 to 85/15 in the presence of a metal catalyst to produce a copolymer; and (2) washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a ratio ranging from 45/55 to 55/45 by volume at a temperature lower than 40˚C, and then drying the copolymer.

Description

-1 BIO-DEGRADABLE/ABSORBABLE POLYMER HAVING REDUCED METAL CATALYST CONTENT, AND PROCESS FOR PRODUCTION THEREOF 5 TECHNICAL FIELD The present invention relates to a biodegradable and bioabsorbable polymer having a low metal catalyst content (residual content), and a process for producing the same. Specifically, the invention relates to a technique for reducing 10 the content of the metal catalyst in the biodegradable and bioabsorbable polymer obtained after synthesis using the metal catalyst. BACKGROUND ART 15 Known examples of biodegradable and bioabsorbable polymers include polylactic acid, polyglycolic acid, polycaprolactone, trimethylene carbonate, polydioxane, copolymers thereof, and the like. They are degradable and absorbable in vivo, and are thus used in medical implant applications such as sutures, 20 bone-joining materials, etc. Since a heavy metal-based catalyst such as tin octylate is widely used for the synthesis of such a polymer compound, the metal catalyst remains in the synthesized polymer compound. When the polymer compound is used as a material for a medical implant 25 application, the metal catalyst is exposed to the body with the degradation of the polymer. The metal catalyst, which varies according to species, may have harmful effects on the human body such as immunotoxicity, genetic toxicity, neurotoxicity, etc. when present at a certain concentration or more. Therefore, when 30 the polymer is used in a medical implant application, the metal catalyst residual content must be reduced as much as possible. On the other hand, polymers for implant applications require features of a certain level or more of molecular weight, strength, etc. In order to obtain such polymers, a metal catalyst 35 of a certain amount or more . must be added during the -2 polymerization process; it is thus required to remove the metal catalyst remaining in the polymer after the polymerization reaction. However, removal of the metal catalyst is not easy, and is often accompanied by industrial difficulties. 5 For example, in a method described in Patent Document 1, a polymer compound is first dissolved in an organic solvent, and a metal catalyst is then removed by reprecipitation. This method, however, requires a large amount of solvent, and causes a drastic drop in molecular weight due to the polymer dissolution. 10 Therefore, this is not appropriate for producing materials (e.g., medical devices) that require strength of a certain level or more. Furthermore, since the polymer tends to contain many air bubbles when reprecipitated, the molded product of the polymer is also likely to contain bubbles. Thus, it is not suitable for 15 industrial manufacture. Patent Document 2 discloses a method for producing copolymers of lactide and E-caprolactone; however, it does not disclose the final metal catalyst content. The publication discloses that the catalyst is used in an amount of 10~7 to 10~3 20 mol/mol relative to the monomers; however, the Examples merely disclose that a catalyst is added in an amount of 10~5 mol (metal content: 22 ppm) per mol of monomer. The further reduction of the metal catalyst content is not specifically disclosed. Patent Document 3 discloses a method for obtaining a 25 biodegradable and bioabsorbable polymer having a high molecular weight by adding 1 to 20 ppm of a metal catalyst and 0.01 to 0.5 wt% of higher alcohol to lactide and caprolactone, and by conducting polymerization under reduced pressure for 10 to 40 days. However, since the end of the polymer obtained by this 30 method is modified with a higher alcohol, it is considered that the polymer has different properties (e.g., absorbability, safety) than previously used bioabsorbable polymers, and thus various examinations are required. Furthermore, since the metal catalyst content used is too small, a long polymerization period 35 is required. It is therefore not industrially preferable.

-3 Patent Document 1: Japanese Unexamined Patent Publication No. S60-501217, see Example I, etc. Patent Document 2: 5 Japanese Unexamined Patent Publication No. H6-501045 Patent Document 3: Japanese Unexamined Patent Publication No. 2000-191753 DISCLOSURE OF THE INVENTION 10 The object of the present invention is to provide a safe biodegradable and bioabsorbable polymer having an extremely low metal catalyst content, while retaining the properties desired for a medical implant or the like; and a process for producing the same. The present invention further aims to provide 15 a method for reducing the content of a metal catalyst in a biodegradable and absorbable polymer that can be applied on an industrial scale. Method for Solving the Problems 20 The present inventors conducted extensive research to achieve the above objects. As a result, they found that a biodegradable and bioabsorbable polymer that is obtained by copolymerizing lactide (lactic acid cyclic dimer) and c caprolactone at a specified molar ratio in the presence of a 25 metal catalyst is capable of having a metal catalyst content of less than 1 ppm in terms of a metal by washing the polymer with the mixed solvent containing acetic acid and isopropanol at a specified volume ratio. Specifically, the present inventors found that the 30 metal catalyst can be effectively removed, without causing a drastic drop in molecular weight, by copolymerizing lactide (lactic acid cyclic dimer) and s-caprolactone at a molar ratio ranging from 40/60 to 60/40 using a metal catalyst to produce a copolymer, and washing the copolymer with a mixed solvent 35 comprising acetic acid and isopropanol at a volume ratio ranging -4 from 25/75 to 45/55, and drying the copolymer. Hereinafter, this invention is sometimes referred to as "First Embodiment". The present inventors further found that the metal catalyst can be effectively removed, without causing a drastic 5 drop in molecular weight, by copolymerizing lactide (lactic acid cyclic dimer) and s-caprolactone at a molar ratio ranging from 65/35 to 85/15 using a metal catalyst to produce a copolymer, and washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a volume ratio ranging from 45/55 to 55/45, 10 and drying the copolymer. Hereinafter, this invention is sometimes referred to as "Second Embodiment". The invention is explained below in full detail. First Embodiment 15 1. Biodegradable and Bioabsorbable Polymer The biodegradable and bioabsorbable polymer of the present invention is a lactide/6-caprolactone copolymer, which comprises lactide and s-caprolactone at a molar ratio ranging from 40/60 to 60/40, and preferably 45/55 to 55/45. 20 The biodegradable and bioabsorbable polymer of the invention has a weight average molecular weight (Mw) of about 50,000 to about 800,000, and particularly about 100,000 to about 500,000. Within the range described above, the polymer is suitably used as a medical implant in view of properties such as 25 strength, degradability, and processability. A metal included in the biodegradable and bioabsorbable polymer is derived from a metal catalyst used in a polymerization reaction for producing the biodegradable and bioabsorbable polymer mentioned below. Examples of such metals include sodium, 30 potassium, aluminium, titanium, zinc, tin, etc. For example, when tin octylate is used in the polymerization reaction, tin will be the main metal contained in the polymer. The biodegradable and bioabsorbable polymer of the present invention has an extremely low metal catalyst content of 35 less than 1 ppm in terms of a metal. The content of the metal -5 catalyst (in terms of a metal) in the polymer is preferably 0.1 to 0.95 ppm, more preferably 0.1 to 0.7 ppm, and further preferably 0.1 to 0.5 ppm. Thus, even when the biodegradable and bioabsorbable polymer of the invention is used as a medical 5 implant, there is little possibility of causing immunotoxicity, genetic toxicity, neurotoxicity, etc. in the human body. The metal catalyst content (in terms of a metal) is measured as follows. A sulfuric acid/nitric acid mixture (1:1, volume ratio) is added to the polymer, and then heated to degrade 10 an organic component. Metal in the resulting mixture is quantified using a plasma emission spectrometry machine with reference to a metal standard solution. Test Example I-1(1) illustrates a measurement example in which tin octylate is used as a catalyst. 15 2. Production of Biodegradable and Bioabsorbable Polymer The biodegradable and bioabsorbable polymer of the present invention having a low metal catalyst content is produced by polymerizing lactide and E-caprolactone in the presence of a 20 metal catalyst to produce a copolymer, washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a volume ratio ranging from 25/75 to 45/55, and drying the copolymer. According to the production method, the metal catalyst content (in terms of a metal) in the biodegradable and 25 bioabsorbable polymer can be reduced to less than 1 ppm. The production method is explained in detail below. (1) Production of Copolymer The copolymer is produced by copolymerizing lactide and c-caprolactone in the presence of a metal catalyst. 30 A lactic acid forming the lactide may be any one of the following: L-form, D-form, or DL-form, but is preferably L-form. Examples of metal catalysts include those containing sodium, potassium, aluminium, titanium, zinc, tin, or like groups. Specific examples include sodium ethoxide, potassium-t-butoxide, 35 triethylaluminum, tetrabutyl titanate, tin octylate (II), tin 2- -6 ethylhexanoate, triphenyltin acetate, tin oxide, dibutyltin oxide, tin oxalate, tin chloride, dibutyltin dilaurate, etc. Of these, tin octylate (II) is preferable in view of reactivity and safety in the polymerization reaction. 5 The used amount of the metal catalyst is about 100 to about 1,000 ppm (about 29 to about 290 ppm in terms of a metal), and preferably about 200 to about 700 ppm (48 to 203 ppm in terms of a metal) relative to the mixture weight content of lactide and c-caprolactone. 10 By using the metal catalyst in the range described above, a copolymer that has a molecular weight, strength, or like properties suitable for implant applications can be produced in a shorter period of time. When the amount of metal catalyst to be added is too small, a large number of monomers remain unreacted, 15 or the reaction requires too much time, resulting in a polymer that is unsuitable for industrial manufacture. Further, a polymer having a large polymerization degree (a high molecular weight) cannot be obtained, and it is thus not preferable. The copolymer can be produced by subjecting lactide and 20 s-caprolactone to a publicly known polymerization reaction such as bulk polymerization, in the presence of a metal catalyst. Specifically, the lactide and s-caprolactone are introduced in a reaction vessel, and then the metal catalyst is added thereto such that the metal catalyst is contained in an amount of about 25 200 to about 700 ppm (48 to 203 ppm in terms of a metal). Bulk polymerization is then carried out under a nitrogen atmosphere, or under reduced pressure according to a known method for 2 to 20 days at 110 to 180 0 C. The weight average molecular weight (Mw) of the 30 resulting lactic acid-s-caprolactone copolymer is about 50,000 to about 800,000, and preferably about 100,000 to about 500,000. At this stage, the content of the metal catalyst (in terms of a metal) in the copolymer is equivalent to the content of the metal derived from the metal catalyst used in the 35 polymerization reaction, i.e., 48 to 203 ppm.

-7 (2) Washing of Copolymer The metal catalyst content (in terms of a metal) is reduced to less than 1 ppm by washing the copolymer obtained above in step (1) in a mixed solvent containing acetic acid and 5 isopropanol at a volume ratio of 25/75 to 45/55, at lower than 40 0 C. First, it is preferable that the copolymer be pulverized using a grinder or the like into grains having an average particle diameter of about 0.3 to about 4 mm in order to 10 improve the washing efficiency of the copolymer with a high metal content. The average particle diameter is measured using the following methods: Screening the particles using sieves having various mesh sizes, and calculating the average particle diameter based on the weight ratio of each of the screened portions; or 15 taking a certain amount of the particles and observing the diameter of each of the particles by means of a microscope. The washing solvent is a mixture comprising acetic acid and isopropanol. The mixed solvent has a function of permeating through the pulverized polymer to allow the acetic acid and metal 20 catalyst to produce a chelate complex, which is to be extracted into the solution. The volume ratio of acetic acid and isopropanol in the mixed solvent is in the range of 25/75 to 45/55, and preferably in the range of 27/73 to 43/57. If necessary, a small amount of ethyl acetate can be added in 25 addition to the isopropanol. In this case, the volume ratio of the isopropanol and the ethyl acetate is about 99/1 to about 70/30. The mixed solvent may be adjusted to have a pH of about 2 to about 6. The content (bath ratio) of the mixed solvent used in 30 the washing is, for example, not less than 1 L, preferably not less than 3 L, and more preferably in the range of about 3 to about 10 L per washing, relative to 1 kg of dry weight of the polymer. The washing method employed is such that the polymer is immersed in the mixed solvent having a temperature of less than 35 40'C, and preferably about 15 to about 30*C, and then stirred.

-8 The mixed solvent is changed 5 times or more, and preferably about 6 to about 12 times. The washing process takes a total of 48 hours or more, and preferably about 48 to about 96 hours. In the early stages of washing, since a large amount of 5 metal catalyst remains in the polymer, it is preferable that the bath ratio of the solvent be relatively increased (for example, about 4 to about 8 L relative to 1 kg of dry weight of the polymer), and/or that the exchange time of the washing solvent be shortened each time. During the latter half of the washing, it is 10 preferable that the bath ratio of the solvent be minimized (about 3 to about 6 L relative to 1 kg of dry weight of the polymer), and/or that the washing time be prolonged. Further, if necessary, it is preferable that the acetic acid be removed washing the polymer with isopropanol to prevent 15 the molecular weight reduction after long storage. The polymer after washing undergoes a drying process. The drying is conducted at about 15 to about 60*C, and preferably about 20 to about 50*C, for 6 hours or more, and preferably for about 10 to 150 hours, to remove an organic solvent. It is 20 preferable that pre-drying be first preformed at about 20 to about 350C for about 10 to about 30 hours to remove isopropanol, and then drying be performed at about 35 to about 50*C for about 40 to about 100 hours. Both drying processes are conducted under normal to reduced pressure (for example, about 0.01 to about 0.1 25 Pa), and are preferably conducted in vacuo at about 0.01 to about 0.05 Pa. The molecular weight reduction of the polymer can be prevented as much as possible by employing such drying conditions. The biodegradable and bioabsorbable polymer of the present invention is produced in the aforementioned process. The 30 metal catalyst content (in terms of a metal) of the biodegradable and bioabsorbable polymer is less than 1 ppm, preferably 0.1 to 0.95 ppm, more preferably 0.1 to 0.7 ppm, and further preferably 0.1 to 0.5 ppm. The weight average molecular weight (Mw) of the 35 biodegradable and bioabsorbable polymer is about 50,000 to about -9 800,000, preferably about 100,000 to about 650,000, and more preferably about 210,000 to about 500,000. Particularly, the retention rate of the weight average molecular weight of the copolymer after washing relative to that before washing is 75% or 5 more, and further 80% or more. According to the method of the present invention, the molecular weight reduction during the washing process can be suppressed as much as possible. 3. Application 10 The biodegradable and bioabsorbable polymer of the present invention has an extremely low metal catalyst content of less than 1 ppm (in terms of a metal), and is safe when embedded in the body. Another feature of the invention is its easy general fabrication. Therefore, it is suitably used as a material for a 15 medical device (a medical implant, etc.) . Examples of medical implants include sutures, bone-joining materials, fracture fixation materials, tissue supplementation materials, tissue reinforcing materials, tissue covering materials, tissue regenerating base materials, tissue prosthetic materials, anti 20 adhesive materials, artificial blood vessels, artificial valves, stents, clips, fiber cloths, hemostatic materials, adhesives, coating agents, etc., which can be made by known production methods. 25 Second Embodiment 1. Biodegradable and Bioabsorbable Polymer The biodegradable and bioabsorbable polymer of the present invention is a lactide/E-caprolactone copolymer, which comprises lactide and e-caprolactone at a molar ratio ranging 30 from 65/35 to 85/15, and preferably 70/30 to 80/20. The biodegradable and bioabsorbable polymer of the invention has a weight average molecular weight (Mw) of about 50,000 to about 800,000, and particularly about 100,000 to about 500,000. Within the range described above, the polymer is 35 suitably used as a medical implant in view of properties such as -10 strength, degradability, and processability. A metal included in the biodegradable and bioabsorbable polymer is derived from a metal catalyst used in a polymerization reaction for producing the biodegradable and bioabsorbable 5 polymer mentioned below. Examples of such metals include sodium, potassium, aluminium, titanium, zinc, tin, etc. For example, when tin octylate is used in the polymerization reaction, tin will be the main metal contained in the polymer. The biodegradable and bioabsorbable polymer of the 10 present invention has an extremely low metal catalyst content of less than 1 ppm in terms of a metal. The content of the metal catalyst (in terms of a metal) in the polymer is preferably 0.1 to 0.95 ppm, more preferably 0.1 to 0.7 ppm, and further preferably 0.1 to 0.5 ppm. Thus, even when the biodegradable and 15 bioabsorbable polymer of the invention is used as a medical implant, there is little possibility of causing immunotoxicity, genetic toxicity, neurotoxicity, etc. in the human body. The metal catalyst content (in terms of a metal) is measured as follows. A sulfuric acid/nitric acid mixture (1:1 20 volume ratio) is added to the polymer, and then heated to degrade an organic component. Metal in the resulting mixture is quantified using a plasma emission spectrometry machine with reference to a metal standard solution. Test Example II-1(1) illustrates a measurement example in which tin octylate is used 25 as a catalyst. 2. Production of Biodegradable and Bioabsorbable Polymer The biodegradable and bioabsorbable polymer of the present invention having a low metal catalyst content is produced 30 by polymerizing lactide and s-caprolactone in the presence of a metal catalyst to produce a copolymer, washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a volume ratio ranging from 45/55 to 55/45, and drying the copolymer. According to the production method, the metal catalyst 35 content (in terms of a metal) in the biodegradable and -11 bioabsorbable polymer can be reduced to less than 1 ppm. The production method is explained in detail below. (1) Production of Copolymer The copolymer is produced by copolymerizing lactide and 5 6-caprolactone in the presence of a metal catalyst. A lactic acid forming the lactide may be any one of the following: L-form, D-form, or DL-form, but is preferably L-form. Examples of metal catalysts include those containing sodium, potassium, aluminium, titanium, zinc, tin, or like groups. 10 Specific examples include sodium ethoxide, potassium-t-butoxide, triethylaluminum, tetrabutyl titanate, tin octylate (TI), tin 2 ethylhexanoate, triphenyltin acetate, tin oxide, dibutyltin oxide, tin oxalate, tin chloride, dibutyltin dilaurate, etc. Of these, tin octylate (II) is preferable in view of reactivity and safety 15 in the polymerization reaction. The used amount of the metal catalyst is about 100 to about 1,000 ppm (about 29 to about 290 ppm in terms of a metal), and preferably about 200 to about 700 ppm (48 to 203 ppm in terms of a metal) relative. to the mixture weight content of lactide and 20 6-caprolactone. By using the metal catalyst in the range described above, a copolymer that has a molecular weight, strength, or like properties suitable for implant applications can be produced in a shorter period of time. When the amount of metal catalyst to be 25 added is too small, a large number of monomers remain unreacted, or the reaction requires too much time, resulting in a polymer that is unsuitable for industrial manufacture. Further, a polymer having a large polymerization degree (a high molecular weight) cannot be obtained, and it is thus not preferable. 30 The copolymer can be produced by subjecting lactide and 6-caprolactone to a publicly known polymerization reaction such as bulk polymerization, in the presence of a metal catalyst. Specifically, the lactide and s-caprolactone are introduced in a reaction vessel, and then the metal catalyst is added thereto 35 such that the metal catalyst is contained in an amount of about -12 200 to about 700 ppm (48 to 203 ppm in terms of a metal). Bulk polymerization is then carried out under a nitrogen atmosphere, or under reduced pressure according to a known method for 2 to 20 days at 110 to 180 0 C. 5 The weight average molecular weight (Mw) of the resulting lactic acid-s-caprolactone copolymer is about 50,000 to about 800,000, and preferably about 100,000 to about 500,000. At this stage, the content of the metal catalyst (in terms of a metal) in the copolymer is equivalent to the content 10 of the metal derived from the metal catalyst used in the polymerization reaction, i.e., 48 to 203 ppm. (2) Washing of Copolymer The metal catalyst content (in terms of a metal) is reduced to less than 1 ppm by washing the copolymer obtained 15 above in step (1) in a mixed solvent containing acetic acid and isopropanol at a volume ratio of 45/55 to 55/45 at lower than 40*C. First, it is preferable that the copolymer be pulverized using a grinder or the like into particles having an average particle diameter of about 0.3 to about 4 mm in order to 20 improve the washing efficiency of the copolymer with a high metal content. The average particle diameter is measured using the following methods: Screening the particles using sieves having various mesh sizes, and calculating the average particle diameter based on the weight ratio of each of the screened portions; or 25 taking a certain amount of the particles, and observing the diameter of each of the particles by means of a microscope. The washing solvent is a mixture comprising acetic acid and isopropanol. The mixed solvent has a function of permeating through the pulverized polymer to allow the acetic acid and metal 30 catalyst to produce a chelate complex, which is to be extracted into the solution. The volume ratio of acetic acid and isopropanol in the mixed solvent is in the range of 45/55 to 55/45, and preferably in the range of 47/53 to 53/47. If necessary, a small amount of ethyl acetate can be added in 35 addition to the isopropanol. In this case, the amount of ethyl -13 acetate is 20% or less by volume, and preferably about 10% by volume relative to the amount of isopropanol. The content (bath ratio) of the mixed solvent used in the washing is, for example, not less than 1 L, preferably not 5 less than 3 L, and more preferably in the range of about 3 to about 10 L per washing, relative to 1 kg of dry weight of the polymer. The washing method employed is such that the polymer is immersed in the mixed solvent having a temperature of less than 40*C, and preferably about 15 to about 30*C, and then stirred. 10 The mixed solvent is changed 4 times or more, and preferably about 5 to about 9 times. The washing process takes a total of 30 hours or more, and preferably about 30 to about 72 hours. In the early stages of washing, since a large amount of metal catalyst remains in the polymer, it is preferable that the 15 bath ratio of the solvent be relatively increased (for example, about 4 to about 8 L relative to 1 kg of dry weight of the polymer), and/or that the exchange time of the washing solvent be shortened each time. During the latter half of the washing, it is preferable that the bath ratio of the solvent be minimized (about 20 3 to about 6 L relative to 1 kg of dry weight of the polymer), and/or that the washing time be prolonged. Further, if necessary, it is preferable that the acetic acid be removed by washing the polymer with isopropanol to prevent the molecular weight reduction after long storage. 25 The polymer after washing undergoes a drying process. The drying is conducted at about 15 to about 600C, and preferably about 20 to about 50*C, for 6 hours or more, and preferably for about 10 to 150 hours, to remove an organic solvent. It is preferable that pre-drying be first performed at about 20 to 30 about 350C for about 10 to about 30 hours to remove isopropanol, and then drying be performed at about 35 to about 50*C for about 40 to about 100 hours. Both drying processes are conducted under normal to reduced pressure (for example, about 0.01 to about 0.1 Pa), and are preferably conducted in vacuo at about 0.01 to about 35 0.05 Pa. The molecular weight reduction of the polymer can be -14 prevented as much as possible by employing such drying conditions. The biodegradable and bioabsorbable polymer of the present invention is produced in the aforementioned process. The metal catalyst content (in terms of a metal) of the biodegradable 5 and bioabsorbable polymer is less than 1 ppm, preferably 0.1 to 0.95 ppm, more preferably 0.1 to 0.7 ppm, and further preferably 0.1 to 0.5 ppm. The weight average molecular weight (Mw) of the biodegradable and bioabsorbable polymer is about 50,000 to about 10 800,000, preferably about 100,000 to about 650,000, and more preferably about 210,000 to about 500,000. Particularly, the retention rate of the weight average molecular weight of the copolymer after washing relative to that before washing is 75% or more, and further 80% or more. According to the method of the 15 present invention, the molecular weight reduction during the washing process can be suppressed as much as possible. 3. Application The biodegradable and bioabsorbable polymer of the 20 present invention has an extremely low metal catalyst content of less than 1 ppm (in terms of a metal), and is safe when embedded in the body. Another feature of the invention is its easy general fabrication. Therefore, it is suitably used as a material for a medical device (a medical implant, etc.) . Examples of medical 25 implants include sutures, bone-joining materials, fracture fixation materials, tissue supplementation materials, tissue reinforcing materials, tissue covering materials, tissue regenerating base materials, tissue prosthetic materials, anti adhesive materials, artificial blood vessels, artificial valves, 30 stents, clips, fiber cloths, hemostatic materials, adhesives, coating agents, etc., which can be made by known production methods. EFFECTS OF THE INVENTION 35 According to the present inventions (First and Second -15 Embodiments), a biodegradable and bioabsorbable polymer having a reduced content of metal derived from a metal catalyst used in a polymerization reaction, and a small reduction in molecular weight can be obtained by washing the polymer obtained after a 5 lactide and s-caprolactone copolymerization reaction with a mixed solvent containing acetic acid and isopropanol at a specified ratio, and drying the polymer. The resulting biodegradable and bioabsorbable polymer is comparable to known polymers in physicochemical properties, and can be processed by a general 10 industrial method. Thus, it is suitably used as a material for a medical application (a medical implant). BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a graph showing the relationship between the 15 drying temperature and the molecular weight retention rate of the polymer obtained in Test Example 1-3. Fig. 2 is a graph showing the relationship between the drying temperature and the molecular weight retention rate of the polymer obtained in Test Example 11-3. 20 BEST MODE FOR CARRYING OUT THE INVENTION The invention will be described in detail below, with reference to Production Examples, Examples, and Test Examples. 25 First Embodiment Production Example I-1 Lactide and s-caprolactone (50:50, molar ratio) was introduced into a reaction glass tube, and 300 ppm of tin octylate (87 ppm in terms of a tin metal) was added thereto. 30 Polymerization was performed under a nitrogen atmosphere using a known method to thereby obtain a polymer having a weight average molecular weight of 400,000. The polymer was pulverized using a grinder into a granulated polymer having a mean particle diameter of 3.0 mm. The amount of tin remaining in the polymer was 80 ppm. 35 The average particle diameter was determined from the -16 weight ratio using sieves having different mesh sizes. Test Example I-1 The polymer obtained in Production Example I-1 was 5 immersed in, per 1 kg by weight of the polymer, 5 L of the mixed solution shown in Table 1, and stirred at 20 0 C for 4 hours using a stirring device. The solution was replaced and stirred for 4 hours. Further, the solution was replaced, and stirred again for 16 hours. This series of procedures was repeated three times. 10 Specifically, the polymer was washed with a solution having the same components nine times, for 72 hours in total. Subsequently, the polymer was immersed in 5 L of isopropanol, and stirred at 20*C for one hour. Further, the solution was replaced, and washed under stirring with isopropanol for one hour. 15 The resulting polymer was vacuum-dried at 300C for 24 hours (0.01 Pa), and then vacuum-dried at 40*C for 48 hours to remove a solvent. The resulting polymer was measured for the metal catalyst content (residual tin content) and molecular weight 20 retention rate. The results are shown in Table 1. The measuring methods are as follows. (1) Measurement of Metal Catalyst Content The resulting polymer was added to a sulfuric acid/nitric acid mixture (1:1, volume ratio), and gradually heated to degrade an 25 organic component. A commercially available tin standard solution (tin chloride dihydrate, produced by Wako Pure Chemical Industries, Ltd.) was used as a standard, and quantified using a plasma emission spectrometry machine (a CID-AP model, produced by Nippon Jarrell-Ash Co. Ltd.). 30 (2) Measurement of Molecular Weight The polymer was dissolved in chloroform, and the weight average molecular weight (Mw) was measured by gel permeation chromatography (GPC) using polystyrene standards. The molecular weight retention rate (%) was obtained by the following formula. 35 The molecular weight retention rate (%) = (the weight -17 average molecular weight of the polymer after washing)/(the weight average molecular weight of the polymer before washing) x 100 Table 1 Volume Ratio of Washing Solvent Status Residual Weight R%) OfTin Retention Acetic Ethyl Polymner Acid Isopropanol Acetate Content Rate Comparative 10% 90% - Swelling C A Example I-1II Comparative 20% 80% - Swelling B A Example 1-2 Example 1-1 30% 70% - Swelling A A Example 1-2 40% 60% - Swelling A A Comparative 50% 50% - Dissolution Example 1-3 _ _____ Example 1-3 30% 65% 5% Swelling A A 5 Residual Tin Content A: less than 1 ppm, B: 1 to less than 6 ppm, C: 6 ppm or more Molecular Weight Retention Rate A: 75 to 100%, B: 60 to less than 75%, C: less than 60% 10 Table 1 reveals that the polymers of Examples I-1 to I 3 had a reduced residual tin content, which was derived from the metal catalyst, of less than 1 ppm, kept a high molecular weight retention rate after washing, and had no appearance problem. 15 Further, there was little change in physical properties before and after washing. Contrarily, in Comparative Examples I-1 and 1-2, the molecular weight retention rate was excellent, but the residual tin content became larger. The polymer in Comparative Example 1-3 20 was dissolved because acetic acid was contained in a large amount. Test Example 1-2 Relationship Between the Washing Temperature and the Residual Tin Content and Molecular Weight Retention Rate 25 The polymer obtained in Production Example I-1 was immersed in, per 1 kg by weight of the polymer, 5 L of the mixed solution of Example I-1 shown in Table 1. Each solution was stirred at 20*C, 30*C, and 40*C for 4 hours using a stirring -18 device. The solution was replaced and stirred for 4 hours. Further, the solution was replaced, and stirred again for 16 hours. This series of procedures was repeated three times. Specifically, the mixture was washed with a solution having the 5 same components nine times for 72 hours in total. Twenty grams of the polymer was sampled during the washing process, specifically, after completion of each of the 3 rd 5 th, 6th, 8th, and 9th washing steps. The polymer sampled was immersed in 100 mL of isopropanol, and stirred at 20*C for one 10 hour using a stirring device. The solution was replaced and stirred for one hour. Specifically, the solution was washed with isopropanol alone for two hours in total. The resulting polymer was vacuum-dried at 30*C for 24 hours (0.01 Pa), and vacuum-dried again at 40*C for 48 hours (0.01 Pa) to remove a solvent. 15 The metal catalyst content (the residual tin content) and the molecular weight retention rate of the resulting polymer were measured. The results are shown in Table 2. The measuring method used was the same as that described in Test Example I-1. Table 2 shows temporal changes of the washing 20 temperature and residual tin content. Table 3 shows temporal changes of the washing temperature and molecular weight retention rate. Table 2 Residual Tin Content (ppm) Time (h) 20*C 300C 400C 0 79 79 79 24 2.8 1.6 1.7 32 1.1 0.5 0.5 48 0.5 0.5 56 72 Diagonal Parts: less than the detection limits (0.5 ppm) 25 -19 Table 3 Molecular Weight Retention Rate(%) Time (h) 20 0 C 30*C 40 0 C 0 100 100 100 24 92 89 71 32 87 86 67 48 83 81 57 56 81 82 51 72 82 75 49 Table 2 reveals that the residual tin content was reduced at any temperature to less than 1 ppm by the washing method of the present invention. The time required for reducing 5 the residual tin content to less than 1 ppm was the shortest when washing was conducted at 40*C; however, Table 3 reveals that the molecular weight was greatly reduced with time at 40*C. On the other hand, Table 2 shows that there was no remarkable difference in the time required to achieve a residual 10 tin content of less than 1 ppm between the washing temperature of 20*C and 30*C. Table 3 shows that the molecular weight retention rate at 20*C was likely higher than at 30*C. Test Example 1-3 15 Relationship Between the Drying Temperature and the Molecular Weight Retention Rate The polymer obtained in Production Example I-1 underwent steps before the drying step in accordance with the washing method of Example I-1 of Test Example I-1. The polymer 20 obtained after washing was dried at 30 0 C for 24 hours, and then vacuum-dried (0.01 Pa) at 400C for 48 hours or at 70*C for 12 hours to remove a solvent. Figure 1 reveals that the molecular weight retention rate of the polymer obtained by drying at 40*C (Example I-1) is 25 82.2%; however, the molecular weight retention rate of the polymer obtained by drying at 700C was greatly reduced to 61.0%. Second Embodiment -20 Production Example II-1 Lactide and s-caprolactone (75:25, molar ratio) was introduced into a reaction glass tube, and 300 ppm of tin octylate (87 ppm in terms of a tin metal) was added thereto. 5 Polymerization was performed under a nitrogen atmosphere using a known method to thereby obtain a polymer having a weight average molecular weight of 700,000. The polymer was pulverized using a grinder into a granulated polymer having a mean particle diameter of 3.0 mm. The amount of tin remaining in the polymer was 80 ppm. 10 The average particle diameter was determined from the weight ratio using sieves having different mesh sizes. Test Example II-1 The polymer obtained in Production Example II-1 was 15 immersed in, per 1 kg by weight of the polymer, 5 L of the mixed solution shown in Table 4, and stirred at 20 0 C for 4 hours using a stirring device. The solution was replaced and stirred for 4 hours. Further, the solution was replaced, and stirred again for 16 hours. This series of procedures was repeated two times. 20 Specifically, the polymer was washed with a solution having the same components six times for 48 hours in total. Subsequently, the polymer was immersed in 5 L of isopropanol, and stirred at 20*C for one hour. Further, the solution was replaced, and washed under stirring with isopropanol for one hour. 25 The resulting polymer was vacuum-dried at 30 0 C for 24 hours (0.01 Pa), and then vacuum-dried at 40*C for 48 hours to remove a solvent. The resulting polymer was measured for the metal catalyst content (residual tin content) and molecular weight. The 30 results are shown in Table 4. The measuring methods are as follows. (1) Measurement of Metal Catalyst Content The resulting polymer was added to a sulfuric acid/nitric acid mixture (1:1, volume ratio), and gradually heated to degrade an 35 organic component. A commercially available tin standard solution -21 (tin chloride dihydrate, produced by Wako Pure Chemical Industries, Ltd.) was used as a standard, and quantified using a plasma emission spectrometry machine (a CID-AP model, produced by Nippon Jarrell-Ash Co. Ltd.). 5 (2) Measurement of Molecular Weight The polymer was dissolved in chloroform, and the weight average molecular weight (Mw) was measured by gel permeation chromatography (GPC) using a polystyrene standard as a standard. The molecular weight retention rate (%) was obtained by the 10 following formula. The molecular weight retention rate (%) = (the weight average molecular weight of the polymer after washing)/(the weight average molecular weight of the polymer before washing) x 100 15 Table 4 Volume Ratio of Washing Solvent (%) Status Residual Molecular Of in Weight Acetic Ethylene Ethyl Polymer Co Retention Acid sopropanol Glycol Acetate Content Rate Example i-1 50% 50% - Dissolution Examparative Example i-2 50% 25% 25% Swelling B C coamplrati-e Example Iri 10% 80% 10% Swelling B A Example i-4 10% 90% - Swelling C A Example II- 30% 70% - Swelling B A Example 11-1 50% 50% - Swelling A A Exmp leat 6 70% 30% Dissolution Residual Tin Content A: less than 1 ppm, B: 1 to less than 6 ppm, C: 6 ppm or more 20 Molecular Weight Retention Rate A: 75 to 100%, B: 60 to less than 75%, C: less than 60% Table 4 reveals that the polymer of Examples II-1 had a reduced residual tin content, which was derived from the metal 25 catalyst, of less than 1 ppm, kept a high molecular weight -22 retention rate after washing, and had no appearance problem. Further, there was little change in physical properties before and after washing. Contrarily, in Comparative Examples 11-2 to 11-5, the 5 residual tin content exceeded 1 ppm, and further the molecular weigh retention rate was greatly reduced in Comparative Example 11-2. The polymer in Comparative Examples II-1 and 11-6 was dissolved. 10 Test Example 11-2 Relationship Between the Washing Temperature and the Residual Tin Content and Molecular Weight Retention Rate The polymer obtained in Production Example II-1 was immersed in, per 1 kg by weight of the polymer, 5 L of the mixed 15 solution of Example II-1 shown in Table 4. Each solution was stirred at 20*C or 40*C for 4 hours using a stirring device. The solution was replaced and stirred for 4 hours. Further, the solution was again replaced, and stirred again for 16 hours. This series of procedures was repeated two times. Specifically, the 20 mixture was washed with a solution having the same components six times for 48 hours in total. Twenty grams of the polymer was sampled during the washing process, specifically, after completion of each of the 2 nd, 3 rd, 5th, and 6 th washing steps. The polymer sampled was immersed 25 in 100 mL of isopropanol, and stirred at 20*C for one hour using a stirring device. The solution was replaced and stirred for one hour. Specifically, the solution was washed with isopropanol alone for two hours in total. The resulting polymer was vacuum dried at 30*C for 24 hours (0.01 Pa), and vacuum-dried again at 30 40*C for 48 hours (0.01 Pa) to remove a solvent. Table 5 shows temporal changes of the washing temperature and residual tin content. Table 6 shows temporal changes of the washing temperature and molecular weight retention rate. 35 -23 Table 5 Residual Tin Content (ppm) Time (h) 200C 40 0 C 0 73 73 8 3.6 1.6 24 1.0 0.4 32 0.5 48 0.5 Table 6 Molecular Weight Retention Rate(%) Time (h) 20*C 40*C 0 100 100 8 92 79 24 90 71 32 91 53 48 95 46 5 Table 5 reveals that the residual tin content was reduced at any temperature to less than 1 ppm by the washing method of the present invention. The time required for reducing the residual tin content to less than 1 ppm was shorter when the washing was conducted at 40*C; however, Table 6 reveals that the 10 molecular weight was reduced with time at 40*C. On the other hand, the molecular weight retention rate was kept at a high rate (90% or more) at a washing temperature of 20*C. Test Example 11-3 15 Relationship Between the Drying Temperature and the Molecular Weight Retention Rate The polymer obtained in Production Example II-1 underwent steps before the drying step in accordance with the washing method of Example II-1 of Test Example II-1. The polymer 20 obtained after washing was dried at 30 0 C for 24 hours, and then vacuum-dried (0.01 Pa) at 40 0 C for 48 hours or at 70*C for 12 hours to remove a solvent.

-24 Figure 2 reveals that the molecular weight retention rate of the polymer obtained by drying at 400C (Example II-1) is 78.7%; however, the molecular weight retention rate of the polymer obtained by drying at 700C is greatly reduced to 54.6%. 5 In the specification the term "comprising" shall be understood to have a broad meaning similar to the term "including" and will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or 10 steps. This definition also applies to variations on the term "comprising" such as "comprise" and "comprises." The reference to any prior art in this specification is not, and should not be taken as an acknowledgement or any form of suggestion that the referenced prior art forms part of the common 15 general knowledge in Australia.

Claims

1. A biodegradable and bioabsorbable polymer comprising a 5 copolymer of lactide and c-caprolactone at a molar ratio ranging from 40/60 to 60/40, or a copolymer of lactide and e-caprolactone at a molar ratio ranging from 65/35 to 85/15, the polymer being produced by polymerizing lactide and E-caprolactone in the presence of a metal catalyst, 10 the polymer having a metal catalyst content of less than 1 ppm in terms of a metal, and the weight average molecular weight of the polymer being 100,000 to 800,000. 15

2. A medical implant comprising the biodegradable and bioabsorbable polymer according to Claim 1.

3. A method for producing a biodegradable and bioabsorbable polymer having a weight average molecular weight of 100,000 to 20 800,000 and a metal catalyst content of less than 1 ppm in terms of a metal, comprising the steps of: (1) copolymerizing lactide and E-caprolactone at a molar ratio ranging from 40/60 to 60/40 in the presence of the metal catalyst to produce a copolymer; and 25 (2) washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a volume ratio ranging from 25/75 to 45/55 at less than 40*C, and drying the copolymer.

4. The method according to Claim 3, wherein a lactic acid forming 30 the lactide in Step (1) is L-form, D-form, or DL-form.

5. The method according to Claim 3 or 4, wherein the temperature of the mixed solvent during the washing in Step (2) is about 15*C to about 30*C. 35 -26

6. The method according to any one of Claims 3 to 5, wherein the mixed solvent is exchanged five times or more, and the washing time is a total of 48 hours or more in Step (2). 5

7. The method according to any one of Claims 3 to 6, wherein the metal catalyst is at least one member selected from the group consisting of tin octylate (II), tin 2-ethylhexanoate, triphenyltin acetate, tin oxide, dibutyltin oxide, tin oxalate, tin chloride, and dibutyltin dilaurate. 10

8. The method according to any one of Claims 3 to 7, wherein the copolymer after washing is vacuum-dried at about 20*C to about 35'C for about 10 to about 30 hours, and then vacuum-dried at about 35*C to about 50*C for about 40 to about 100 hours. 15

9. A method of reducing the content of a metal catalyst in a biodegradable and bioabsorbable polymer having a weight average molecular weight of 100,000 to 800,000 to less than 1 ppm in terms of a metal comprising the steps of: 20 (1) copolymerizing lactide and s-caprolactone at a molar ratio ranging from 40/60 to 60/40 in the presence of the metal catalyst to produce a copolymer; and (2) washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a volume ratio ranging 25 from 25/75 to 45/55 at less than 40*C, and drying the copolymer.

10. A method for producing a medical implant comprising the steps of: (1) copolymerizing lactide and E-caprolactone at a 30 molar ratio ranging from 40/60 to 60/40 in the presence of a metal catalyst to produce a copolymer; (2) washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a volume ratio ranging from 25/75 to 45/55 at less than 400C, and drying the copolymer, 35 so that the metal catalyst content becomes less than 1 ppm in -27 terms of a metal; and (3) forming the copolymer having a weight average molecular weight of 100,000 to 800,000 obtained in Step (2) into the medical implant. 5

11. The method according to Claim 10, wherein the medical implant is selected from the group consisting of sutures, bone-joining materials, fracture fixation materials, tissue supplementation materials, tissue reinforcing materials, tissue covering 10 materials, tissue regenerating base materials, tissue prosthetic materials, anti-adhesive materials, artificial blood vessels, artificial valves, stents, clips, fiber cloths, hemostatic materials, adhesives, and coating agents. 15

12. A method for producing a biodegradable and bioabsorbable polymer having a weight average molecular weight of 100,000 to 800, 000 and a metal catalyst content of less than 1 ppm in terms of a metal comprising the steps of: (1) copolymerizing lactide and E-caprolactone at a molar 20 ratio ranging from 65/35 to 85/15 in the presence of the metal catalyst to produce a copolymer; and (2) washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a volume ratio ranging from 45/55 to 55/45 at less than 40*C, and drying the copolymer. 25

13. The method according to Claim 12, wherein a lactic acid forming the lactide in Step (1) is L-form, D-form, or DL-form.

14. The method according to Claim 12 or 13, wherein the 30 temperature of the mixed solvent during washing in Step (2) is about 15 0 C to about 30 0 C.

15. The method according to any one of Claims 12 to 14, wherein the mixed solvent is exchanged four times or more, and the 35 washing time is a total of 30 hours or more in Step (2). -28

16. The method according to any one of Claims 12 to 15, wherein the metal catalyst is at least one member selected from the group consisting of tin octylate (II), tin 2-ethylhexanoate, 5 triphenyltin acetate, tin oxide, dibutyltin oxide, tin oxalate, tin chloride, and dibutyltin dilaurate.

17. The method according to any one of Claims 12 to 16, wherein the copolymer after washing is vacuum-dried at about 20*C to about 10 35*C for about 10 to about 30 hours, and then vacuum-dried at about 35*C to about 50*C for about 40 to about 100 hours.

18. A method of reducing the content of a metal catalyst in a biodegradable and bioabsorbable polymer having a weight average 15 molecular weight of 100,000 to 800,000 to less than 1 ppm in terms of a metal comprising the steps of: (1) copolymerizing lactide and E-caprolactone at a molar ratio ranging from 65/35 to 85/15 in the presence of the metal catalyst to produce a copolymer; and 20 (2) washing the copolymer with a mixed solvent comprising acetic acid and isopropanol at a volume ratio ranging from 45/55 to 55/45 at less than 40*C, and drying the copolymer.

19. A method for producing a medical implant comprising the steps 25 of: (1) copolymerizing lactide and s-caprolactone at a molar ratio ranging from 65/35 to 85/15 in the presence of a metal catalyst to produce a copolymer; (2) washing the copolymer with a mixed solvent 30 comprising acetic acid and isopropanol at a volume ratio ranging from 45/55 to 55/45 at less than 400C, and drying the copolymer, so that the metal catalyst content becomes less than 1 ppm in terms of a metal; and (3) forming the copolymer having a weight average 35 molecular weight of 100,000 to 800,000 obtained in Step (2) into -29 the medical implant.

20. The method according to Claim 19, wherein the medical implant is selected form the group consisting of sutures, bone-joining 5 materials, fracture fixation materials, tissue supplementation materials, tissue reinforcing materials, tissue covering materials, tissue regenerating base materials, tissue prosthetic materials, anti-adhesive materials, artificial blood vessels, artificial valves, stents, clips, fiber cloths, hemostatic 10 materials, adhesives, and coating agents.