AU2012216795B2 - Soy depigmenting and skin care compositions - Google Patents
Soy depigmenting and skin care compositions Download PDFInfo
- Publication number
- AU2012216795B2 AU2012216795B2 AU2012216795A AU2012216795A AU2012216795B2 AU 2012216795 B2 AU2012216795 B2 AU 2012216795B2 AU 2012216795 A AU2012216795 A AU 2012216795A AU 2012216795 A AU2012216795 A AU 2012216795A AU 2012216795 B2 AU2012216795 B2 AU 2012216795B2
- Authority
- AU
- Australia
- Prior art keywords
- skin
- product
- soy
- stabilising system
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 9
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 9
- 239000002738 chelating agent Substances 0.000 claims abstract description 8
- 235000013322 soy milk Nutrition 0.000 claims description 15
- 230000003019 stabilising effect Effects 0.000 claims description 15
- 238000011282 treatment Methods 0.000 claims description 11
- 239000003755 preservative agent Substances 0.000 claims description 8
- 230000002335 preservative effect Effects 0.000 claims description 8
- 230000002265 prevention Effects 0.000 claims description 8
- 208000002874 Acne Vulgaris Diseases 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 239000004365 Protease Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 206010000496 acne Diseases 0.000 claims description 7
- 230000002401 inhibitory effect Effects 0.000 claims description 7
- 239000004094 surface-active agent Substances 0.000 claims description 7
- 230000001965 increasing effect Effects 0.000 claims description 6
- 230000037394 skin elasticity Effects 0.000 claims description 6
- 230000009759 skin aging Effects 0.000 claims description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical group OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 4
- 230000037380 skin damage Effects 0.000 claims description 4
- 239000002562 thickening agent Substances 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims 2
- 230000000699 topical effect Effects 0.000 abstract description 2
- 230000000087 stabilizing effect Effects 0.000 abstract 2
- 210000003491 skin Anatomy 0.000 description 36
- 235000010469 Glycine max Nutrition 0.000 description 28
- 208000035484 Cellulite Diseases 0.000 description 7
- 206010049752 Peau d'orange Diseases 0.000 description 7
- 230000036232 cellulite Effects 0.000 description 7
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 244000068988 Glycine max Species 0.000 description 5
- 102000003425 Tyrosinase Human genes 0.000 description 3
- 108060008724 Tyrosinase Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 210000000577 adipose tissue Anatomy 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000035614 depigmentation Effects 0.000 description 3
- 210000002752 melanocyte Anatomy 0.000 description 3
- 230000003061 melanogenesis Effects 0.000 description 3
- NTQVODZUQIATFS-WAUHAFJUSA-N (2s)-2-[[(2s)-6-amino-2-[[2-[[(2s,3s)-2-[[(2s)-2-[[(2s)-2-amino-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]-3-methylpentanoyl]amino]acetyl]amino]hexanoyl]amino]-3-methylbutanoic acid Chemical compound OC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O NTQVODZUQIATFS-WAUHAFJUSA-N 0.000 description 2
- 102100030310 5,6-dihydroxyindole-2-carboxylic acid oxidase Human genes 0.000 description 2
- 101710163881 5,6-dihydroxyindole-2-carboxylic acid oxidase Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 102100037132 Proteinase-activated receptor 2 Human genes 0.000 description 2
- 101710121435 Proteinase-activated receptor 2 Proteins 0.000 description 2
- 101710173693 Short transient receptor potential channel 1 Proteins 0.000 description 2
- 241000282898 Sus scrofa Species 0.000 description 2
- LVTKHGUGBGNBPL-UHFFFAOYSA-N Trp-P-1 Chemical compound N1C2=CC=CC=C2C2=C1C(C)=C(N)N=C2C LVTKHGUGBGNBPL-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000007794 irritation Effects 0.000 description 2
- 210000002510 keratinocyte Anatomy 0.000 description 2
- 210000002780 melanosome Anatomy 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000037393 skin firmness Effects 0.000 description 2
- LKKMLIBUAXYLOY-UHFFFAOYSA-N 3-Amino-1-methyl-5H-pyrido[4,3-b]indole Chemical compound N1C2=CC=CC=C2C2=C1C=C(N)N=C2C LKKMLIBUAXYLOY-UHFFFAOYSA-N 0.000 description 1
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 description 1
- 241001415166 Alona Species 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 1
- 102000016942 Elastin Human genes 0.000 description 1
- 108010014258 Elastin Proteins 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 101710088172 HTH-type transcriptional regulator RipA Proteins 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 101150011508 Ighg1 gene Proteins 0.000 description 1
- 102100031413 L-dopachrome tautomerase Human genes 0.000 description 1
- 101710093778 L-dopachrome tautomerase Proteins 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001401545 Masoas Species 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
- 206010033733 Papule Diseases 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 241001425800 Pipa Species 0.000 description 1
- 241000350481 Pterogyne nitens Species 0.000 description 1
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 description 1
- 101710173694 Short transient receptor potential channel 2 Proteins 0.000 description 1
- 206010040829 Skin discolouration Diseases 0.000 description 1
- 241000862632 Soja Species 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 235000009430 Thespesia populnea Nutrition 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 229940071160 cocoate Drugs 0.000 description 1
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 1
- 210000001787 dendrite Anatomy 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- IZFHEQBZOYJLPK-UHFFFAOYSA-N dihydrolipoic acid Chemical compound OC(=O)CCCCC(S)CCS IZFHEQBZOYJLPK-UHFFFAOYSA-N 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 229920002549 elastin Polymers 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000003810 hyperpigmentation Effects 0.000 description 1
- 208000000069 hyperpigmentation Diseases 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 210000002414 leg Anatomy 0.000 description 1
- 238000007443 liposuction Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000015250 liver sausages Nutrition 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000000933 neural crest Anatomy 0.000 description 1
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000007310 pathophysiology Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- NQLVQOSNDJXLKG-UHFFFAOYSA-N prosulfocarb Chemical compound CCCN(CCC)C(=O)SCC1=CC=CC=C1 NQLVQOSNDJXLKG-UHFFFAOYSA-N 0.000 description 1
- 238000010814 radioimmunoprecipitation assay Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000036548 skin texture Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229960001295 tocopherol Drugs 0.000 description 1
- 229930003799 tocopherol Natural products 0.000 description 1
- 235000010384 tocopherol Nutrition 0.000 description 1
- 239000011732 tocopherol Substances 0.000 description 1
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a skin care composition for the topical delivery of a soy product comprising anon-denatured soy product and a stabilizing system, the stabilizing 5 system comprising a member selected from the group consisting of an antioxidant, a chelating agent, or apreservative. This invention also relates to methods of'usmg tfie1 skin care compositions of this invention for treating skin conditions and improving the appearance and feel of the skin.
Description
AUSTRALIA PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT ORIGINAL Name of Applicant: Johnson & Johnson Consumer Companies, Inc. Actual Inventors: Miri Seiberg and Jue-Chen Liu and Stanley S Shapiro and John Kung and Rachel Grossman and Jonathan Miller Address for Service is: SHELSTON IP 60 Margaret Street Telephone No: (02) 9777 1111 SYDNEY NSW 2000 Facsimile No. (02) 9241 4666 CCN: 3710000352 Attorney Code: SW Invention Title: Soy depigmenting and skin care compositions Details of Original Application No. 2009212783 dated 21 Aug 2009 The following statement is a full description of this invention, including the best method of performing it known to me/us: File: 32230AUP03 -la SOY DEPIGMENTING AND SKIN CARE COMOSMIONS The present application is a divisional application of Australian Application No. 14433/01, which is incorporated in its entirety herein by reference. 5. Field of the .Invention This invention relates to non derlatured soy product-containing compositions that can be used fordepigmentation, evening out skin tone and skin texture, skin firning, and care of the skin. More particularly, this invention relates to the use of soybean 10 containing compositions for depigmentation, producing skin of even tone and colour, increasing skin elasticity and reducing skin aging, increasing skin firmning and promoting a younger look of the skin, for the prevention and treatment of sun-induced damage,for shine control, for the treatment and prevention of acne, treating cellulite and for inducing aesthetically pleasing skin feel. 15 Background of the Iuvention The understanding of the chemical and enzymatic basis ofmelanogenesis is heavily documented. Melanocytes migrate from the embryonal neural crest into the skin to produce secretary granules, melanosome, which produce melanin. Melanogenesis occurs within the melanosome, and the melanin is later distributed to keratinocytes via 20 the melanocyte dendrites. The key enzyme in melanogenesis is tyrosinase, which initiates a cascade of reactions that convert tyrosine to the biopolimer melanin. Two tyrosinase-related proteins (TRPs) are known, TRP-1 and TRP-2. These proteins share with tyrosinase about 40% homology and have catalytic activities as well as regulatory roles in melanogenesis. TRP-1 is the most abundant glycoprotein in melanocytes. 25 Skin colouring has been of concern to human beings for many years. In particular, the ability to remove hyperpigmentation, such as found in age spots, freckles or ageing skin, is generally of interest to individuals who desire a uniform complexion. In certain areas of the world, general body whitening is desirable, There have been many methods proposed to accomplish depigmentation. For example, kojic acid, hydroquinone, 30 retinoids and other chemical compounds have been used for depigmentation. Many of these previous solutions have not been found acceptable. There is often a distinct line of demarcation between the areas ofskin to which such previous compositions have been applied. Therefore, precise ov . io~ia .bU -a I-:Ucy& 0. l'4 h - ,
.
J ' -r .lox pi enti naof uil.er mousi k i nemla-ry A.order - e dsu4 tesult.-xwn of .btse compo udsy e a e d be iig - 4intnd thereby -4 -DdeSbIC foruse. - -h e ;-a -'tmaeous tdisrersas welft th-tapeudi4alerve~doea. Xowte the ndgdyi&a - -eck'ant'snW and 4he tablt ip4dsid7 sh~qw1er devef ping tdirinfh nnry hyper-..jaa -4 *genttin- ri .wlunderdtood.Tuls'dadaer.tegarmed aV b ~ effecdy* for -cdrmpoudiilnrdeinfa, or 15osr--4infiid aory pigkdtad~fi ax arfd tul~ays., -~~ -e aidabfr, yjdqindqnne andiboad-ipeytzm sunlcen ae T9'ke. ef -1luagptic tre tmen l , bur thy q ot cdqiptesy prevynt o tad . prgntfdigy 1esi s, A gefctive ad fi al re - ot - -smt E4 1. A t d , in.b.rh -h~ n is th 6y - ' e ep n o&46t-o id4s.d to,.
.. IaL*csed*.ere a<cia"s'u en sm *nldn~~- ~ ,tinbj,,pg the : 4al o of -4. .se'lt ot an, 4 s. * ca e s :i a kat(-. t g a - . . fes *s icre. dealcucs p toatshpsy dlaycg th p tk ofth ski 3. d . - . '~ .
. .. Q- 'h o az 30 2 = b; b gce ed n y ssuenof grea casocialsigal senc o nad-conc n n'd a'yWbedomersid 22 - gg h x fcs4de a cone 4l,~ne~n e at~nan . . A;'~.
1 *rd -ni a n nieiabi .c.yyora spoc n 25 rI idApprsz$te44kc 4?. blsssgeosinpd ttin.s atfr S~hpi oh straossp eri'zoe an.h's fU Ri h rameto a~~kndsae - ,ae ed. icesd reyle et oaingpsduinga thernpde~c es abIding jby e oue of topia oreatis whi~i~icldqbe irrit n and ,xpeSbves ner a - * - ;4 .1 4 .4 1 - old af-y9mil 9i.t - - -. .* it-h deusthhayeagia4patenhr tt p 1 .*e Y 9 -- aingtd pr-o E yreect* oyt4Iaffrdrm~iiatt.' to -, - w g&e anid'ffr, l r1t raiy n 're, - d.* 4 f4s, withho kyiaua ornigaites defets, and.whii .'fl&xerPrvde - - -' the de~sii'ed-hoge eoui Skin'tqgpplex o- een .. igte<a*( n d a . 'e9or9 pp a *. - i - ,di4k aadr~%wgctoh -. * *.:c; - - - .,ran n h . -g, eIcts , b en r sn, nd ad fg t -,. , - any d sca z a rgjei ii caigjidd ca'6fhis re r a Be Ter 'pJed -j-sigb,.wahig ad'segsi99t p*yieitckiv 'be e .pae. - - -r e 2u.hg.'asigl n wodalestdicleThp ily cai chk lJprvi * 5' idf6theek .-ouf ehomo e s in copleion, e i fla ttu n'oe 'ad dc skia fr. id a il ier lok.
3 i pus . ip le o f at i4 4f 4th . Th anhdiruttcs is o nlj, aph n f4tn b me 1 yfr - -9 .. 9 * - - 3 -* theictj., 6;1i aso einbx sng, 2nd cwo fttslr In dnfipremfpt 4 s*4 A9 wi aA f2 i ~4aa9djffc~xn aProA~et't spieeitdorde ill been.c a-ep u'iyj thy pastjWig4e ifmoczteb84s snm lexio,pag 6.rt *r. ru a ol (. c iiiuc ski UJ9 ,.d 2. 9q~ a look. 36. h .*ii 9. th hgsadbmcsis9 nh , * b ~s dc$iu4$ ci 4h a c wb t) rac, fit .lljt 9. 2 A,. * cn, hz 84zr e~ie phxn~urc4~esri'ibL t :nbe~~r& 3 -4 basic pathophysiology of cellulite has not been clearly identified. Theoretically, cellulite could reflect differences in adipose tissue biochemistry or connective tissue structure of affected versus unaffected individuals or regions within an individual. There is no evidence of any primary role for adipose tissue physiology, blood flow, or biochemistry in the etiology of 5 cellulite, although the connective tissue of the female thigh is structured to accentuate differences in small sub-dermal adipose tissue depots. Products aimed to cure cellulite (e.g. Cellasene) have been marketed all over the world, but their efficacy is in doubt. Methods to treat cellulite like the solid-probe ultrasound-assisted lipoplasty technique or syringe liposculpture result in some body contouring but are painful and expensive, and needed 10 repeated treatments. It is desired to have a topical affordable treatment that could prevent or reverse cellulite and induce skin firming, resulting in a healthier, younger look of the skin, and provide daily skin care with no irritation or negative side effects. In our previously filed PCT Patent Application WO 99/04752, the use of non-pasteurized soybean-derived extracts for the purpose of depigmenting skin was described. We have now 15 found new stabilized and cosmetically superior compositions containing such soy products that contain less than 0.1% surfactants, have greater skin-depigmenting activity and are less expensive to manufacture. We have unexpectedly found that soybean-containing compositions have anti-aging activity, they better increase skin elasticity and firmness, and they are useful in the prevention and in the treatment of sun-induced damage and acne. 20 Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field. It is an object of the present invention to overcome or ameliorate at least one of the disadvantages of the prior art, or to provide a useful alternative. 25 Summary of the Invention According to a first aspect, the invention provides a cosmetic method for increasing skin firming and skin elasticity and reducing signs of skin aging comprising applying a skin composition consisting essentially of a non-denatured soy milk having protease inhibitory activity, a stabilising system, said stabilising system consisting of an antioxidant, a chelating -5 agent and a preservative; and a thickening agent, topically to the skin, wherein the composition contains no more than about 0.1% of a surfactant. According to a second aspect, the invention provides use of soy product comprising a non-denatured soy milk having protease inhibitory activity and a stabilising system, said 5 stabilising system comprising an antioxidant, a chelating agent or preservative, in the manufacture of a composition for the prevention or treatment of sun-induced skin damage or acne, wherein the composition contains no more than about 0.1% of a surfactant. According to a third aspect, the invention provides a soy product comprising a non denatured soy milk having protease inhibitory activity and a stabilising system, said stabilising 10 system, comprising an antioxidant, a chelating agent or preservative for use in the prevention or treatment of sun-induced skin damage or acne, wherein the composition contains no more than about 0.1% of a surfactant. Unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an inclusive sense as 15 opposed to an exclusive or exhaustive sense; that is to say, in the sense of "including, but not limited to". The present invention also features a method of depigmenting the skin of a mammal, said method comprising the step of applying to the skin the above compositions. The present invention also describes a method to enhance the care of the skin. We 20 unexpectedly found that application of soymilk or soybean-derived compositions increase skin firmness and elasticity, even tone and texture, reduce skin aging, and are useful in the prevention and in the treatment of sun-induced damage and acne. This invention provides examples of soy-derived compositions useful for skin care. Other features and advantages of the present invention will be apparent from the detailed 25 description of the invention and from the claims. Brief Description of the Figures Figure 1 shows three graphs, demonstrating that soymilk can inhibit trypsin, inhibit PAR-2 cleavage, and inhibit keratinocyte phagocytosis.
- 5a Figure 2 shows historical sections of swine skin treated with soy formulations and stained to demonstrate pigment deposition. Figure 3 shows a picture of a flank of a dark skinned swine, treated with soymilk and DHLA formulations. 5 Figure 4 shows histological sections of mice skins treated with soymilk and stained to document elastin fibers. Figure 5 shows pictures of an individual excessively exposed to the sun, following treatment of a half of his face with soymilk.
-ly -. - S - r 1S. gareve hhd tis *nan c t and drancy stafl4 dgipi? - r 00.5 -- -. re83 o .4 y r.l o . 4 - 3 . .3, 6 .vfii k94rxcv te1 10 - Deta~~-ile DeriptiafeN s d.m d h - S'.eiv hto s laip.i r n pa h .. - .u zes4ywsep pvede t 91 t~s d i dhduze ~e#xO-c -h -- -* * -*' -z-a- 4f-gnhiy?ir w s je.m-Ul. s .*' -- * uneitadb -oto a d y! #i intpihocc hsa M - ... Idc . ,. - . * - .: '*L - 5 . q. . - .. * : - , , e .- -L - - ~the any O poitiop 6f thisnviincnti leguwie r,)dus an" * - - 3". b. . .. * - - preferabl SOY i o d~i nP MY~ b-Nfo(fa fAild (oj;yu)r 2Ls Aid 5. ben p.owd. r ik p r Wt S n y -"soy prd0tjis A - aitace erie~fo ndesqybein, contitch indeftn1youa in 23 soy beaws thesitv conbdntratigg a s fIn~d in Ti,. beius;n ti odimni:pth , . SL.53 -5 . 6piodua isApo-deugired say proglucjt "Deniarurnde g isdeliti-theBDantamn t*.:*s* *.J3) an. d *j .. . - M.'cai .cti.t eit s "the change in the ph 4 andhp soogS - preiserties df a pio'itan, that a'br'ought about by heal' kiAY O~r & als, Thlete cemgeq inecllwude loss of actvt (n the case-'of enzymes) and los (or altation) o 3d iieiiy( h taeo nies" Wbgt ismeant bT "nwdenabied soy peoddke is a soy produce in which the: proceing for the derivaion of such soy ptoduct (e,g, n - ,. *5 .ill e - r - * - -r - - - . . - . .bib d e Oinp 14.1 1101C k pedia) did got areisrtae7 7 - ~ ~ ~ ~ ; - Q of .tis , *- ..- t- * ead by ;4 pr.ei~ned of an intact scbirisi nhibiorn .3~~~ j 3 - -- nano her e.dirpent, the sdyprbduct is sop.AkI. Ciae .f? C - - . . -. .. -.p *. -il - hi. proies allow t ieat ear -ie & h dybdna eyze diirt ditozrnme raoye d~ibtoyaCzty * h csO e in entrokn & rsh fximei6vx di; pare4d a: indact oAiu itiaoh mog -A 50 g ' au t rozml groun tesd'd ybets ussg jib a - -- - -% ro~tase inhi y-cti i, t ihe oyb p inre reie - Thi~on as in o U irb rn-v ention b .feay,'coti r ott 3,A .
- e . . . ; W -t 'I. ~i~h?:1 b 9. b Ven syrm d.t.(~a. s milo s the-3 i nromau t.oyj o hut ./ayw.ht( oiaot 30 2 toabot 3%,.b wdght wfth oioyprduxt. 1ompo.tion pich cumprises -olidsot p iro ecyseay ao cmprike wtr4 (g distald w tero r ercwoa1i e ~jJ. no soyrtulkf in, theedat42n * IO fiii t.4d'Q.L.T~ Th- uddiisth*~iu at eth io irn j3d frox pIotay be j;;V sotiybn od rhiyAfld.4tae rtain~oved,~vklxile r~n ~K:::x. -. oi fC&P s; tIiqto 1. -o~m *.fro4ni~ 3TI .t k6 ivnhzl0d1 ira dibcd atift of,, the soy rouc -~q .~~c 3.a S.4 qu dsyVd.a.' C 33 . . ~ Q?$1L~ tna,~t~mkM wder~ or spyrdiI LkW94 asid z ~pao tiL 30.t abou 3 n cb .3 .3 i Jrby~gr of. th soi soy prdu.C~nostaA soidso 1.oui'm als 3op~ wae (e3g.. 1iM a ut~;o wa3r3 3 .43.fe a~~A. it '.Mn.#a~~4A7 # is - - 3 *hi s'oymilk) to jo-Hn liquid base to the to4slsiton (eg., o fdrni.a cr e lodo n- iectable so0iitioa or gd): Such comtpoiOil p my toprpses fro about 50% to b16- -e, by'ieifht (e. frin ;bo& 7b% to about 98%, by weight) ,Pwer .Whie ad - - ~ .. i th bese tirtthods pf aininttarou, dhe cm p positionss ob ave ion may. be - -e ed togie y, r a -. . - - - -3*;*-* -OY- -0 - o -' fu o hr p it1i; Vena. y pr S a -r sy ->:dF&rd fal ea c jdgln * h wpare. in ex t16 Hawr specific stpg, g dgrpekis drig!is or gowth condo idnsI jhb pde- - -f-giW - .oyf gig~buoiuotli thig: dzai qs t produe r io art *grl - ~ ckv - hrdpiiEhIitor (e.g S'lif gl BNI do7' r rbd i h -C Ixndys"rihibifoibhak in t he 6a =p b'ip ;sd -1 - d that dThe leg -bidutusflirhdenps sof s u6 h ye - 1 -; r iv 'r' i n e a ne soy taridcfth erifro geopicbngWstrubo eI~s h~avns ci 1 cn4 .. gd das*4p 4afuig but noY big to lipyrab eedle gn bkd adoh e-0g Iraulycipecimi strip reduothgrs iongs rosin aged'nts rfrgranc b&a alson-4? to heusdtoeas heoorM - pssv vs. tu t'hefact th9, th ornyesWihs ofthiinA ai n a rrrtwn r - d r p o Shthe ph j ' t d) eflfo ubtnial pdenigmirbild coposhion.gEpmtplbferesiatve - -5, 3 *delI de phnre*ae anaabn uh smtykaae, ty *aabn and The compositon may'ctoorife from aoui:0.01%.toaboYt 20%, , ei i (mor -w Iqt44t r.-'A~nA-uhkrhqrnfi cukMuxp ru Lleaq o17 b4ed W h ierz prodd the usdil An pr' ~Q4itLn fds a n6 ~e~-~ '*5 C grp Si.!a 1 es. .. 3. v3.. .tc th t nv t5La *ei inodiflc4 aigarpro like. .p sai Tb rd d ox)filil.I ( C a0 'emusd on Csei :1 odor. 'xo4ah rfe antf DiifS tand £~abk &W~ i= '~gjn(M j7be., Ss)'eeixfe reere ;a 3' as 'h tse~ j'. ,~o Thesqvmpxies.o Du tbrape from about 0thq ton~io -aofc(/o inviiffVcai' (ore~ 3 . 3,- .3. .3e A. 3! 3y - pretehbly4 from aboni-0.5% t6abu.%byeih)ofpseav. erbl coa -ro eva bio byr gnaaG io y ;be readwe tt hatfdp idt result in the'eliiniation of protest iphikkoy -- Antiez dm 4ndgX 1dei ggd~i also bo ui 4 b3. p3iiobse'Adoicrbeci$ ou,4 - . - -. ce d .340tb and kcy - -ei - .\''. . ,v..3--.3 -s -. - . , - , . & .* - - iainbat Nat~iye cnd, eeg o,-L aufass ikb fiesg h - - -6& I. T. do. - - -- atdi i i emi' d Oi b n Ii e to,' bn i m sg : 'S. ~lf 4-4n ?.O~ 6' 1 6 sdnmtwdi rx~dand N.ec "i4j 27 '* ,0.sg d 0id tdihei a dai;s(eg i li daz n) - 3 r, tant rtraolanU.4 th.ca. c 14 of s f fal gicti IjludIgbr' t e ca piria .bk'pipa Hs4a legung ex.E tr#apeq- 2hxd M rAy b e fou4i Og pes 1612ij Ojhe Casmie Hi6 Lk -e7-6 -- e SQ11 of r6 mal10tenton hy' p Visne'ni~d a about a 00 )4 abr.u 0 -b devvige (Vg frd-a 0.a about 1rbj - . wtipasiaan.6- C-m- - -o - .e C-hea* .gaden bure afo sduls in b4ling the roytldo if the compositions of3s ion Piples chelaig agnt includeI)TAsgd delivatives teref eg;isdumD~d dipoUss EdTA); hdfeine '*, lActefe #, dCdeIT*I ca -~~p Odraupl'es of hpeatidv agnt arelistedon page a66o h Ca=in igned -$ 7 .. co upositirnsyf pofniire fq nhvenrion e ricomprs hea ceKtai.igsagent9Isn .. - . -9 - - -p-e * .- - -. noabf~rmoug 9.p016 to~i 20o bI by*kg rdde (e. ~ t r-1 f * .s . I.' .s). td 0 dsto . 9;13 - - Thiciagn dg ~. knr'r 4ahidcmg at t -4-d M cjE poas 6a f vend a td lter the r ty es acre a . thed e6t41 .-tt471 peduad itnen a ath>r I - - 3 3 - e , .' ' ' ' ' ' . . .-. * . - - 9 k *.h-.q . . * 00 cps'asd .. AddiJs) . a - - - - - -t----t -el d ~ 1Agent.1a io adaud 0j teo o -f -~~ - -'5bL ri pix 'ly r a - xPles of viscagity niodify s areii4O opgg19 hCsr - ot k-To 4uisvthe ap roprj~ si aS cLii t - - - - .. . . . . 9 .3. --_T ecoEarpoofhis iuiion. qg 2 inddirdon rLo the sy pi-bdii -r.Exples'of ych depgnpiggns ir.e - - i iiied to, lipoic aed, dihydrolipoic acdrevo.rtroggiggbiC id Oli , .- - - - e osinkse inhibtott, tndaaosopt tfr inhibitors, and'selctivecydbeaets6 -i lanocytes, or aaurafexxta~cs'santiaIeg these actividea. The amoiuc of i -Z2S dimungaetAe didpedp he actlyLt-y of the com~poptind, apd' will - . tpicaly'rh.5efroi aiu' 0.601I% to abit Im%; 6)y wdight (&g', q aboxk 0.01% to .3e, - ,,. . mabo(g10%, by wdight) o.f the compositon.r.a-t* TIlicongo&id of rche present' uvi.ndojmay M a s coiti'cg Tohedsird 30. aee -ihe fAel of the composition on the skn of the user,. uEmples af bit compounds include, but are iot limited to, oils, silicones (e.g., silowgnepolymes such 9 9 - - 1 2 0~-' 93 -- s*" :di.hicone) trid zsin-coniioning agents such as 0xiollients, gdhntaefran - - E p6es d'Such sklx condioing ageurs may be faond.of p es165 6 44 7 P fthe In I -Eismrntic HaLdbook. - * -.-. *.-6 - . .-. , -.. - -. . - . *t .e tA~±i4i9f this inivention LAY sliA'' renodan the. lnct 2 f ic ud bp;t dot lirnifrd~o 4n~r~~ ~ d~ - 8-oids*and ts Of othe*pt tpk age ti ay 4v .4.4n th sk-p, inciiding am.i-y n ents nh s c6rdcs odsgtics d a dxy acids co-nryeQ d er an-Libiip lq id antwd h - -x -cV ctd prodicts mstisudin v is co pt h -ik and-otlierskarkpirto Ehos*of nmy! s iai r a- et d oabiadq d# ~s tdiiti i xearid 21 C O so isk rgi V ihlh hld g . e ... o taodsd s.oenxdnteth' sfndeo, cinld e> -8 -~ ~ ~ ~ ~ dmur my4 h de d enio - - dos.Thedom'psitiosoftxynion a'be: pyaei b, de acs a -, p ab ea t.wFe eya Taip ohe i. Aa thp a44h&s analpn pb alA pake,'a Io in tuea l bus iis 4 d furher enhnc prdtdtbliyi jt'I) kkrW rt t p J fp ti ii~ -ar'ndo id f8,t 8* Sa' 88. -n'te n n m a buetd i n - h skn mmah oll ip (egf *Bijn .. hat: manby~dpgetto ;s42jg o- - 30 Jai,~ /orpeo (eg.ehefce n or who o MIch 'Aft a rs oo dkr to dmy br 8 pa tholonyn the in I~ ~ ~ ~ n- * 8.'Arpsq slo th pen ivu . , C& ifrlnrc rot exsaioi& , vmTiic ian ydpimpm is th ot "0,tf gbe pi*ezf iciteqdtn a4eprpzed uez f ardoi i y r pg .o,Qnthe*)ue~'w 't.
-4.. .. ' .. '. ''. 4- -. ''4 i C)"o. hi fi Ez~pofgspotsziIksor p d aressuh is, bu o Iinited ta, psc4l ory hypet-pigmentary ** .. s -. -4" 5? vlu zoul~na b u'decmomn 2 -. " * idiis pA.rCy . 0hdm- '."? ptorea ~je t kpp To~iC41i? O ~ix'' 1 . .- e , ' - -rori).d - -- -a-. - -W eI ~U C T CC 2. - -. -4n p gya he pp.opi xdt th be c~ to 2rva rV~ k -' 2-4- - - * (CX 10 9 'qrfe~ ebtwtMccmpo±ni Of~ bg a 1mI1ige(ad 4eaorcilo' p r e 0 u~ l .
. All IoAtt~ter~.anr5s th desrdefc. ''''c'~' '-- - .ra : - ''* . . - 9. Pos en 1od' tpPJyJ - i5 day ora erd'of time prefer iiipea abb45 day adh'A d a . - 4 .f . . . * ' 4 ', ' 4' . . . * - ppprVeft tn dle qppai-aC e OiLyf dlie cotidijo - Rmldwvei , ih h r'aplication'of heonsto Lb~ oi t'-I fii- prvui i o de -oppgi pr *, - of the iadrd64s salbeduslaxd ketiviry - ' ' .I r . S'othe Ueznribndimnr we founa thai soy ptoduvz, itcht bt'no biw - . ohe coinfetidhs hinvemiouwhich cdntainprge8e ilhukir7 WhCU - - pd topicallj the skin, [nchiding it1 ai' a bath additiv', ori a pate' as4sty n cre#Iig oohlng soqiit, and/'4 reax nteffect for the tsenadp a sdeic-l -? --4 - aipi skidt4-he rpnonecar pfo orhposirionainofte sn Cnprion (e.4 thcoynilk), The ;ondhicn of the skin adea being treated, and the dIesred effect - .' and,.th lls, irU',4tithately be deernied by the user of the com oI'i one . ' -- nb ebdxm& , the cpmpoirson is administered at last' once daily fras odni as the use 30 antshe desired effect. '12- r - -rotg d unexpetddly found that spyrnlk .eari h a -fo 'rog Z b essive exposnre-td the s , relatedd the pai 4 b :g feen;s d- - tedede skinsltiess associaed'with swnbura it imn k provides a ierhod - ~ ~ M-m - o gesthn coS fg.aE pai reusn effe foll i g buti deesiv i - -& - -feTibm dd ' t'nsl~tid'al toi0'ateea least esq per4 y for - ~ onga .keus.wais the desire ef o orik an ng cornpositions ha - ptain.pteae in bi 33.n t S Ter# d .nt fde y tp ly:fed hat p relint. bu -. , pp -l-bicary d&u iuyJ , e Uhd P - -L -'7u2r4t ek~ f . ,'ofsdy:d do t ng - -g- e,..x- pd #ts xp ved eyr-f ask;:arr .ir e r~tos cliidin - 332 s /ubod old enaireglbax', dei'entn kdegn4d texur 3f3th 3 .AM-)Vk kilt743 - .-. s nJ 2 e pot-i8-m'n or hIperp~ gaieutedon 4 ca ;ui cellu ii ti. *~ c - -e ,, . . , , , of * '- -.
- - ervide ed-by tih pev&iuspfes rein 4eghave veexptlyfothatk t exce snvLsnxet'%"ely foqtqhoiaayppii of oy4rd prd uces. -~~~ rk nvnla PT Piri Applictio WO9/45) hc otinpyioihltr isi 3 sd r *Vct licsusd;/bpagdo oirFsdxpik'to heiiu '3p x4s 'o :b c ei pO L 4.Y ''. d 4n.l 4abistit, pre:&=~ scjscou~dat paiop4plc~n of oi4 epird9th u afrgdc sxu/urlifg u Co On w tesaoi; Ipmdatjo Of eSiven ~on &d t1xre of theL a' 6 g' e pdt rs ~Sy aI in sk*n incrgint ekic,=1m~ rperiz esrsfa'# .
- anterjiral'y pleSIing skit fe.I'9sigventioP provides method ro i I-ymfag and skin elast;yapd reducing sips of sn agi which - - fuloLai l bodr paft, and pefeoablyfbr tie ace upper bddr d ofhe skin a - -highs ad Ab'its. ri erhmd itss of-daiy ;0pien: bnt& daily fr a2 Log $ .s Tel sr Want the Ipsacd efi4eof 4kco- -" -- positizr h Ca pIpT e'bnbibitr ard t -.- - - - - e4 ~~-on.' they ould aqt be utilie'd td Eau rhrrwt cpi h .3, ihve n- 440'g ~ ~ ~ ~ x-f dei idae-h egfieri or hfopr. 0ie nle'W then sied'hugodhwe v ole 5 C - -d i nevr.Thfik thuy should in'ibout 1. td if or n, . - -, - - - Ea npe.2 Peaaio bf-SOy(milk Get frem Sbymflk - - Tli~d following composidog o this inventon weie prepired 2s (olow,1 h 20 ?vight peicetexs of each ingredient in Ihe :ompitionsui- in~cat?efo-n -~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~11 -*U~h - T1e v gi.al .Frt &ynW a ieae . iaye sced into ;I ,L ." 3, . . I.. 3* * 3. p *e. Thi 3.9 ~ 7* - *l ak.Th prsgaivePecie( ueo the resevauei miedt '~ - ~piraben, yropyl-parben; ety ~arabn aind Phenoxy-ev4ROl-sol0Y NI±A, thington, Deaware)'ar thopreservTe phenoxye4 r tnol 2 w dd to the 25 Soymaill Nei%, the chelanng agent Disodfum EDTA an~d in some ,xM~ple the - - ' humeci glycerin were added to the fur beaker and rnixed with the symilk. h63s - -Corning 200 Fid 6), orPySrbate 20, or Aluminbfn $taeb O tccina!, or 'Suvrose Cocoate, or FEG-6 Captic/Caprylic Triglycekides to the s~ym n.ie r ;30 thisep as rquired in some mmplesin Table- and Table 2, E mi-e.ofthe , thickener polyacry.dde, laurerh-7, and T13-14 isoparffins (sold by SeP pi., Paris* 19 France uader dheTrdenam. Sepigel) was added to a second be' a4dong1 wit the - anti-oxidar BHT. The ingredeAts in the second beaker were thet added to the ingredients 'f the first beer and mixed untU horiogenous: The an-oxidants - £ . istoric acid, tdium iscorbyl phosphae,lactferrn, or tocopherol were then add d - ' o th beaker and horageneously rnaied to form fhe.resdting e -. - * -. ong. MpoNi omsof tIi v s - - - rth S4 Noyt pwder (D"; 1soyFartns,arrol n-osvde-- -. * :Ttf o s :r Ve~n . -th k :' -; ~ f -wake P~ xd li9 alts bek nddrcOdstitut4 e yiesdiDErA.w tnnw -. 5 the t b'dr41e md £ngi the csofk A mikpur Fi I y duf 1' - -eT 'PTr ingjdisovsint nd beai "ere rhen added to the ngrdiens 6 sdeaki di4,antl-ho Onus. .5 .
37 j 3 7L 3 3 S.3 . ' v i~.!~'.c ;_ Is'll33j, * 0. .%~Ut~eIN 21 2 3 8742 8Q04 960. *3s/j . *.. An'h9 -~ ~ 3 3 .66%3 3DA. .3 61uri I .3.'.Q 61 8ftgd -0%g00 g . Poim.3at .. .;. 3. 33 *3 . 3 ~ 3. 3. 3 . - , r. 5 6 TOTAL3 3* 3 ~~3 100% 1 009. 3or: 311j -. 3 -,3:'-..'. . . ' ,. 2 - 3 3 a d - Gi ~ph.'. .'. ....- :,," . - - .07 23 33 P3.of i.ll;. ndh gg spinn.83 469 .- 3?. 32 .% 2 Aj tCA... -' -fl . - - f-- - - -- A .. 3 ig'P '3L'b3-J -- J-l 33 E' -. Of%~ - . - . .92-0 - - - - g4-0% 9 - idudief:7::.,.EY ''- -C .. 00% -t b6dfmhiEiTfst (n sr Mu - - N -6 r *, -J Tf TAO,, -J5 -i~l: .%0% D Od~ 1004 160*. T1 -3-g7-. 3 2 - %-k: ioAA% .7!k hId~zdhod*r- 4r. 0*a.. Exmpl 11S k'hi100P~ {?r - -f nlagl-hs20 efeFonbepi-sin g.edn gae a iow] o r pon *.ityd3&vet' -". - W -ArY. ' - ' ' 'r- . ..1 - -.. e~n E asy4iwziod dephinn ned Xtssis e. .1.$n -* s. in ote3Z10 .bteDb 10t0tfpn 1ce.cea 1 oY lo%'ece~ 3 -uc eo s (o d Or 3oi3te . Thn ;logie.tr gi nuatd ad t ;:"e(/v ith 00 nk o rfsi epe i.P-- co9peaQur fr onesc~ gour dursee as measuredi (eiatin yt 50/m SS I .3~ . . . . -oy- 5pcrMa * d min microrp e pl i-ndr(M lclrD vde'oyrin fUdg fand*Pro . soffwpera 4 Moleculari Cororaio Each experience 17 -'1 33 .... 6. .I- . .Esmtus SMown il Sodnwnt the. PA R2 Pahw 99/30729isfdn'uitclt*I Yv wit 3OO roieAr~nin4 ddntk iBft 1 ~; r.~o -~i Bbbifrp mn Gpsgnieicast=~~ flu' L awIFylg AALvLf wing .o Pr *,O .oewr 3e oprtol.F Uxeiet -e ned4 ~giin -keicares and was egeed there times, The pertertt inu - - - ncva of thesubsra;e by ST1nd soyn calculat gc Mlcos'i :rcel 4o graphedsin-Sigina r,. As shown lf.ine AZ symbil cn ni......t A... grU'apr r.A-*nL. .. , -typsin-induced leivage i4 a dose feipbuse manter. - - - - - ageced egeriaedt miarassy was designedWafps *d thi *i;y-A t.~3 - scer;.IpibtIc, of soymilk on jAR-2eag1 ths ya nhd -ur -cent peptid compi lvage Sie osei * Q 1 *y is esgd in: .ourbakos Chnpn t. s , J,9aci ikW.N Clavage abd-activatirio prtin4ag enp :- i4. tivn stryophiiI2IiawP fi omi rphay .
4 ha . i ~ 4 -k $qi -5 i 1, Sii,998), rp.14ced, he cpeain gfots-':.n - i, ea pep -wa oamidd with the lycofi4e p p i n hi- naida ad'aie's aqfghs.trate fo VA-*2i - - -w -~kl toO Pjtiey.A10.2 skcayatordi spe ect4 d uidiet e eid i 15); The LC i indipbrt - adied hAd- pgp;e dle-dage by S'It og SqYnilk was measure by ib~ ng 00 3.. - (201.32peptide Wihiounits of arypsin (in 100,reM TRL I
-
ydolinthl~a~inin'tae buffer pH 8.0, 15ee,4 v 1 ,'ih of, -i e T wi I. -tt : ru the restiafrirhal, for oneihour =n room tamperature protected f-oyli ,t -, _Arbcene wasme1uredn spectr * Ge9ini "ilredplttrireadeai qbre e .
-- earDvesCrprad) usa-ota*Po3.0 toffi/arq'gdo'ir ' - Deice Copoiton), ach~expedient ivi perforliadd 1 six rleplidates andl Was - rept~ied three Eih*es. The perce nt inlhibti'noftrpiclage'daeae s S5 - raphdin S Pt. . As - - s . pid .y trypsi ivas completely inhibited in Ters o0 ' - Soy'i ip iped the iltyto inhibit this trypsia-ind4iced PAR-2 cleag (Ft -. 2b), ugge't that sirila to S77;soybean x.racts could ithbi r.. .2ac o .&30 To examine the possibility that soy'mill cold inhibit PAB.-2 induced keroinocyte phagotytoss*' used the Vybrant Phagocytis Assay (lvoletular ..- ~4 3 ± * ~* 3~ 3 3~ - - 18...--.t..sd~ **- .3 - .
* * ide, nd ineleindncasfe jis of SIor sdymnflk f24 hours 4lowig by - .. £4 ti 4d -i . -~ icqb*d'n wilOP Oicro ire of ilndres9en abded-, coli K-i iipa-ricles for- £oiihonri btxacellulv fluotekcence, Indicative of ingested tdctjpar$.le was
-
.e *m .. .. . , " - - j~ * 0 Iten - - Eg ) Si 4n620 SPSS Sceiac io~f re - -ped ( e
-
* .- 2I.e sc - riiw ik p~ats amed s s n ~ t tu~- .s di me- - 1 6dg "f -p to 3Yai . 3 A *' * -q 3 ': ~ ~S S . * -3 - . 4 -a ue- k nb" -*. l . * * - . .
. r . 1 9 . . - -** .1 .. .. *I Qtir paent appficatin -WO 99/0452 locimenss the' ep ing tlty S f soymilk, and prepnts soyilk-onrainingformuado for th u of depzgminwadan. An Cmple of-idh formuiadon jis Soy ELsente 1; as dgs4bad !A'*. - T-1e aboye ti! prdsen iavedu a de aibes de ipenting cmpoo -6 temof ateriused i cse in-Tfs it a ty S db udlida iht Soy Ns'eic 23N sierOr to Essea6e-1 ig it $int - a dl wing experikdnt was performeL.. -I -- *r .s n Yudatan midof9Wihe (Qhadde r t ) -prop -w ptoseinpWhr ete. iet.es in-'ad cd w - bpts@Aiw foo?'drs vatar dllbitur: Ad1t1i tite w -~n . j -~ - !5 "5kQe'-for the: Ca #bte b. aboratIysa r NIH 6,. tia!op 85-23-. TWLety hibrefIfe tF ts opotnds Wer - fryJled tpic.ywc ad.ay-fiv f-igt r ir w kIC 6n. i: dorsm t ih . Tfea renof ihdidua swir were a1Wegs ,arraigd InA a head tW ti'oerop e'e idb, arid In! a . * -.- 20 tal J hegd bNrJarj thd other stde oftthe- ahiriaIt'.epsih were aken using sthtard tethtiques. A wino sAldi-sshiketi no vlstyal Irritain nd hiatelgical analyses-reveiled r rifket irritation or other Peth6Iogjca!- sigrIs. 2Sesions ia bIosies *tre gained with orona..MasoA ($4 ) 2 ad#dre-re (She indHgk,1 :& s g erfi v .4 5 h 08) .&I tia -dn sQ - --"rreducig-roiecal In si, ~tis-pifc stain identi R' nt 7 ,Ian Arec thieias per biopsjwere ptcess&. Each ezpereent was repeated at - 1= es thre'times. - 30.- - -. ur'2 hoe~/stl5cilecon tro91n t'i bish rIhets,, -:.e sectiapr - Ee inea v Rtb FW, to mark tdinii4anin deposinio in the redcsie& The - . toP.aalhowSr control (a) and the Soy Essence23 (b) treated sites of.on swin-c. Th J*cr panel.'showi tlie'control (c) and the Soy Sssence1 (d) treated sitet of . 20 24cid 'si'd It is d6Kids'-fron 0higure q- hadhothi 4ed the - bolute reductibn iiedeostion idtedbSEsn 2r to - -1abelevelinduced by $oy EssenceL - -4 tioxiaik. . . -. - I: ut partnd Appic 0.99 752 douiqengthd e PXpq ug activiy-- - - -- %.i ibitjon el PAli-2 inidaied Icentidjocy phgoyog, eortr- tsh- - tp2 v: pigntig6tn ObUaddfv iif. innckd 2iianL yiM ont SwineSeke trAt'd'with s k tr S*Flk. n~e rSiat ous .1 - - , , '., .. .. t . 15- ifdro spoic.acid (LAad1ReraolRe)twicedal,,rth'- C- - .- ' . s -i m n c d .a1fSian f a q e - - - -- Ve fogl ing gwek traqtet w ith'pp lb6 rkisg aridD 20 combination.. Thepleftt ae(1) i -dwi 5ea ?ritllti desdibed abbow -The right site (a)s treaed withbli., i Q~- .. S Soml Esen 20 h T. * iyid~tdl g ( Pis- irea eed t boftiSofilk phsence 24 azs D tLA TI~t~sz rh .~~,. -a vydses 'hckeased depimentatio follow t of So'ymilk ssee 23 d el to treatmin(;swit eachiagrdet alona. 25 Similar rpesulis were' 0tained using soymijc*of sOgmilk essence coinbined -with HLAor Res. Thistxampe-eeadydemosrtha-addtio different meihanism can improved the deigmethig effect of soydink.
. . m 7SOv.m.ilk affects ski' elasti 30 Wiille performing the saydIk Swdies described in our a.pplida.o*nsJB 30 -YBP-464 andflt--47, we unexpectedly notceil that the skin of soymil treated miceC . ... /- 21 - - --- 'Iek more elas'r., .To tthe posi2ilitf ti toy .
-_a.w p to SEA sehts fl edin nsno2.n.invasv- -- ty.lneSsurmnfnts (ct tetermsuremen) andstfnipg of skisecnon,for. 1i~. q. g ' * - - * . -- ta-fiber. Thies caiijndiare ihat 4bymilk treaminainIcreates fws. - - add .nd*= tid tiesied kin h=s increased elad'rres lm- - Gf l 571 mcavingo 4herct'frane l'ar-. cl sa e gri r - -4 piio B.6adP ,or sbiVedi dw - sio f (f " * . . " -. . e . -. - Caifo!dia) asl dngloyed ihehdse decribdri..ggAa te lg.h- -- - - -o - ai Ben.b iP prdwesdn.),o Evausiit d of1 B&ne of Ag N)i d di b - -. - - 2 .. .2 -o *s &i ' Skin .nd TechAp. 6a'-7(1995)id Elsderit6,. - - -. 4ncalro d of nign oeatadVuivir Ski;22.? J.enmuci. - -- 61 (1.9 )4ich~af -bodi ingbrporated kexia by refeigot~e if-thtde wet -Suti a e h wia e ad tdi correpni skin ds .2byery fpi the oad 4/U ( gcd tc, or eksde rnceve f loadi. -- - a Ur/e ($itutespr' imromvealA i ;he deformationrliacafesi) i2isa bettetvoniity add ejgiy o h s he de2fom - -' aand Orare 80lapendent, in put, on asthikaess.. Conseq4enrjy, ra ias a~re used - - Onkualu(aiou as described. in BaretadL, "Sucdon Me thd for lisurehipeA of Skin 5 -Meaica''roprti..The Cutornecer," Handbook of Non-lnvgasive lferhods and t6e. - ,kg 3 ( ch is inorpoined heein a eteci enirt. - --. An exa lofa oe such'*.perimen*is*shown inTable3dem ridn - i miIj reandent increases these paraiteres, which reflect itar skiri'eat Ab .'hile varia ons biaseon animd were significant, the increase in chrom*ic* .properdes was Piostent, reflectng the bienical effect of somi* to skin e laty 2*2. . 2 . ! B s a D27i - D#f21 - --- - - -- - io netet
--
- - Uhaieated'Symi k: litrekt'd Soyh - -- T Uf A.~A/, 15+-089t/-0$ - 0.0 . . 0354 . 0( ' O04-- - - .r/U -' 043 - 0.964/. / 1*4929 - S -. . + - 13 so 151~ .L.2 t - K'f2) U36a4 bei. p e 9.00. 8 -4 el reqexd b 4 tsve rety. - -a -r -4 aue tir q b 913 (s n' d'k b. p. big: '&t 6rrd n CokaNol Tei * -,-F*ta Q ice igh4± '-a do/ v s ' i **.', " .. . 1 . ** . - ) . Tnc1 '.a Q ary t ti.&etorth- 6e .I a -5- ' Tjasegahr~d xp-' ,thjt e egctf' y pyAder on li.e 1 sf noIlw: emlrrbas w*sesdb evaluating'thqedorp'orario 'o d ra*acie' .lieinetr' lilr olae aftjZ te Th~ii~e$A days f ojfgretme tr. . . .th*: rew.n - - '-ibols a gm by 584?t.o.rcneneton noIin ~~ .. .3' . 23 e - - Ak iho**L nFiureV4i Miim fi~g*(Siaed d~t Li * TJ , t* tka * m *:i ap 'upr .dmi, c . ~~t*: (Ik~~ad~Onmi luc gthtpesIicmpfd to unrd:cnot M14 it var dd~a ~ arrthe o -ddly 6f v i . ah *j5 'sai cdisistetziirhodglwut tht ictird. Saxcrsun.4k ~'g 4 -me "O dei~ r htesus'O aSo'otattji i4 CI57BV/6 mice. &ontasi; gf sotuk and toy ul forijiolacious fdr skin catqWol in~rdak dum 74ks7 arat exxseniixf kl, the efec o -9~&Von couAgeq s Ae:sswa -10 stUJ4 in tir:Th fvco16 powder . :aq~ the' [Ar .0 in Table 4; say powder enha~nced The rawe of co11agen synxaesis 1n 'oxrnll denat-fibrobhs at I Vg/tnl b? 5861. At lowt exr concczitzssAtiouij, no Sigmaifiku 23 - -- i.thtlon wa seed with. yO po ddr.- us, h z ea Ad hr ful i; -. youthful'- 3 - -k Ta-lc. pea n r ' -an damafbrobl -a n 8 .3 . . .. '. . S i(s /- - - 3 -. - n -v n k $ it. 3ind?{ d2 - - Safi~ firiikl - - - -or -y-ur- -- *6 t - - 3 3 . .- - . stba 4 e ttol soatreyt neafdth *..tpti * g - . - d 3 iebrol4 retonxtcy;.ai[o1I'de sypnc~ iniru -MnW - - -; e rt. - - i. ,p- . dual's *t 3 .'C ski . . Ite kh .m io " n - b 6jto 4Aa~odiness w;yf44v~oped bydeod and thrhdatemlAoe - -/ecAdlyI jh r Udet.6 s'f 0e ag' beei 1t1a8.d- s. i e duls min Uoosrtgrhesnnd of eutetd il d injns - -oppiAin acitn to yj fhsieton l poe thi e skin u iie dt Srmmrrxi6& IA. 1... 64 -3-5 '3brnst'' 6vrp 9:y c an pret sut--n et detn -T - 4 po Tipa= .-. 3 .p s ro ny td geds * ~~~~~.3th '3rnrnohaiwgosldk'ou thef~c tiht A f1'fdQ2&dyi idztl f~ C4'~"W rxpa d och iz 1 lkU tra T hea~ siiriry winSW4c the usi h d iane r e .. 3 . 'L .A..LLt..L ) * . "-t:" 4xet*i scise .wEm i e fdviaawatTe if tw s in A r cieus d iw derc cotnitdam ac avosC i i '3 Sao] ;ii m reuerdei eagles 6~ pain~ Wosiatea e wit the3. evi-g wm onsratghosaueto U 3 trb MidviU ct. eaglbe intrtl 7aloudat sd* anrdpth snofness pin ~ C &i4 dhon~ die' "c I 3.c ti a 42d4 3ou o unexusere TheV7L41U 6 .30 hsxonrk~ su-xI~l tomr i s;ven to;n oidy iji p rvtea the; h in xuerncr 1C * 3 ~ 24 fodaii Mlude-olipg anxd soorim senyaon ta relieve thetpdi asiocadwithth-. . -s and tq reduce giess.id'scqidesi whi leviga "fresh - di'ge ' This eMgp4 demonstiates that topics apjicaptin of z ynil orsoym r n ould relieve the pal and reduce'he 'redness and senlihek. tesukedfrorn - e.esua e~posure The ause'o 0' ymais on sungpse sin 01 t gn rabsensatoin *~ . - - *o 33.eke r- -5~ .A5 ca- idy v? goail de in stdy.he e e app - - - ea.aar ah infps. - " - r'Of 6Ix study) =§d h'pyri' %10ct 1g og e .A4 S 3. 3*etD4*a i photo. *S - e .T t a QCD INGharpe W*f a highly siiiatdecash p' ff e .in -i . . paui 0.00j),4. 11i3 also a Srecinnerend wairta ees ii) erythe *ia'(p.0p6) frOde bgelid' no athe - 20- . b,, tere r .sdife'at'ide'se n conedoaes, papules or ptitles: Thei c e -ed aub4 qj y -ul Is ilustmra phidally inL oigur-7 hum~p apppyx4 ap cat- ' &4mle .6 o~ilkontinn.Cmuo Akmsof hi Inuidt nMini:,Atact. 2 n- Thdnsdynik-conian a coiposiions df this ivenid .re no-dnated - * - . d l- r. 5 -and have prqtea*-einhibitory acavity" Thesd crntpos4idotis are un ebcuete - congen intact porteaits, and M pardclar ina St a eepiotee idibior- *To dnn tthisuniu eness,'qualo sofFssence23,describiabovein n''pie 3, A f -hecay av-ale soy-ased srroduct (Helen I naisteid's FurWreifsenc, clab g "soja profeins" in thtir ingredierts)O-di weecOup2And - Clk-STI Co3en;. wing Western bloi'ccording to Bo*ifacio J.S (ed:)99 25 -.. f.nunnuorednce n:INIng. In Oritud todl Call BiOlOY Oh Cla ROhi - Jhri Snsduc. (reton .Da ay1.es, J.knW 3i4 1s .~r I'" . 3 l. IWA..bifforlt% ioulder-P401 0.5% odimd c 0.1% sedii decedy.l' 33 sulfate, ai d Coapla protease inhibitors (Beh~dager yndn'ln is - - IN-in PA. RIPA, sale (10 Eg pr Iani) -were elcn- horesO i % P.AGt -- s er adayv by'lence le $xtnscrrireL weSThf .* - - cl -. i tA di to $ we h -C ho ~ ~ ~ ~ ~ 4t con'and~w eE Lsii25a iui WOE 1:5O 'Iai4 * * 1 ~;. e sjo 1 - -AssMd'gialgure 8 ouryfot4ulition E nce 13 tbNtus mncg $ - * - - -. I-3 3.W 3 ZA.I.a M . 10.. .chr'(t .ch is run iin as -* - ie er, the plab~o of.ssec 23, hor the. mmer Pioy-base pwdct antais. -3 - - 3 tSTL . -~ -- * - - - - -'; - .~.~: -- - Iis , rdartood th hiI t iridikas b e&e'desild n eriN W*ththe d de sci dP tofth rhc io i decrit 4iis ianded 6 -S 'ntdanbc-flznAt t bo e df thn Vlteptoi, cIh a defi nythc idte of - de ap -dyd a Other aspetrs advitages; an odifctie arb wi;hit the - c lams - .. 3 I *. * II . e *I . *% 20
Claims (15)
1. A cosmetic method for increasing skin firming and skin elasticity and reducing signs of skin aging comprising applying a skin composition consisting essentially of a non-denatured soy milk having protease inhibitory activity, a stabilising system, said stabilising system consisting of an antioxidant, a chelating agent and a preservative; and a thickening agent, topically to the skin, wherein the composition contains no more than about 0.1% of a surfactant.
2. Use of soy product comprising a non-denatured soy milk having protease inhibitory activity and a stabilising system, said stabilising system comprising an antioxidant, a chelating agent or preservative, in the manufacture of a composition for the prevention or treatment of sun-induced skin damage or acne, wherein the composition contains no more than about 0.1% of a surfactant.
3. A soy product comprising a non-denatured soy milk having protease inhibitory activity and a stabilising system, said stabilising system, comprising an antioxidant, a chelating agent or preservative for use in the prevention or treatment of sun-induced skin damage or acne, wherein the composition contains no more than about 0.1% of a surfactant.
4. The use of claim 2 or product of claim 3, wherein the composition comprises an antioxidant.
5. The method of claim 1, or use or product of claim 4, wherein said antioxidant is BHT.
6. The use or product of any one of the preceding claims, wherein said composition comprises a chelating agent.
7. The method of claim 1, or use or product of claim 6, wherein said chelating agent is EDTA or a derivative thereof.
8. The use or product of any one of the preceding claims, wherein said composition comprises a preservative.
9. The method of claim 1, or use or product of claim 8, wherein said preservative is a paraben. - 28
10. The method, use or product of any one of the preceding claims, wherein said stabilising system comprises BHT, EDTA or a derivative thereof and a paraben.
11. The use or product of any one of the preceding claims, wherein the composition further comprises a thickening agent.
12. The use or product of any one of claims 1 to 10, wherein the composition consists essentially of said non-denatured soy milk and said stabilising system.
13. A cosmetic method for increasing skin firming and skin elasticity and reducing signs of skin aging substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples.
14. Use of soy product comprising a non-denatured soy milk having protease inhibitory activity and a stabilising system substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples.
15. A soy product substantially as herein described with reference to any one of the embodiments of the invention illustrated in the accompanying drawings and/or examples.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2012216795A AU2012216795B2 (en) | 1999-11-05 | 2012-09-12 | Soy depigmenting and skin care compositions |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US60163906 | 1999-11-05 | ||
| US60201494 | 2000-05-03 | ||
| US09698454 | 2000-10-27 | ||
| AU2009212783A AU2009212783A1 (en) | 1999-11-05 | 2009-08-21 | Soy depigmenting and skin care compositions |
| AU2012216795A AU2012216795B2 (en) | 1999-11-05 | 2012-09-12 | Soy depigmenting and skin care compositions |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2009212783A Division AU2009212783A1 (en) | 1999-11-05 | 2009-08-21 | Soy depigmenting and skin care compositions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012216795A1 AU2012216795A1 (en) | 2012-10-04 |
| AU2012216795B2 true AU2012216795B2 (en) | 2015-07-09 |
Family
ID=46940679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012216795A Ceased AU2012216795B2 (en) | 1999-11-05 | 2012-09-12 | Soy depigmenting and skin care compositions |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2012216795B2 (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5322839A (en) * | 1991-09-13 | 1994-06-21 | Pentapharm Ag | Protein fraction for cosmetic and dermatology care of the skin |
| WO1996028008A2 (en) * | 1996-03-19 | 1996-09-19 | Guerlain S.A. | Novel cosmetic or dermatological compositions for controlling skin ageing |
| WO1999004752A2 (en) * | 1997-07-28 | 1999-02-04 | Johnson & Johnson Consumer Companies, Inc. | Methods for treating skin pigmentation |
| WO1999036050A1 (en) * | 1997-12-24 | 1999-07-22 | Novogen Research Pty. Ltd. | Compositions and method for protecting skin from uv induced immunosuppression and skin damage |
-
2012
- 2012-09-12 AU AU2012216795A patent/AU2012216795B2/en not_active Ceased
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5322839A (en) * | 1991-09-13 | 1994-06-21 | Pentapharm Ag | Protein fraction for cosmetic and dermatology care of the skin |
| WO1996028008A2 (en) * | 1996-03-19 | 1996-09-19 | Guerlain S.A. | Novel cosmetic or dermatological compositions for controlling skin ageing |
| WO1999004752A2 (en) * | 1997-07-28 | 1999-02-04 | Johnson & Johnson Consumer Companies, Inc. | Methods for treating skin pigmentation |
| WO1999036050A1 (en) * | 1997-12-24 | 1999-07-22 | Novogen Research Pty. Ltd. | Compositions and method for protecting skin from uv induced immunosuppression and skin damage |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2012216795A1 (en) | 2012-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2038097A1 (en) | Method and composition for coiontophoresis | |
| US8147815B2 (en) | Topical administration carrier composition and therapeutic formulations comprising same | |
| MD1699F2 (en) | Aqueous composition of a human somatotropic hormone and process for preventing denaturation thereof | |
| AU5857498A (en) | Cosmetic preparations containing extracts from phyllantus emblica and centella asiatica and/or bacopa monnieri | |
| CA2358958A1 (en) | Soy depigmenting and skin care compositions | |
| US4508707A (en) | Hair tonic composition | |
| IL110121A0 (en) | Stable finished formulations for crop protection and their preparation | |
| WO2001037782A3 (en) | Method and composition for the treatment of scars | |
| DK0841899T3 (en) | Preparations for topical administration of active ingredients | |
| GEP19991852B (en) | Composition for Protection the Plants, their Applying and Method of Increasing the Yield of Plant | |
| AU4347397A (en) | Composition for treating acne | |
| WO2001030802A3 (en) | Therapeutic treatments comprising administration of certain steroids for blood cell deficiencies | |
| CA2082505A1 (en) | Method of treating alopecia | |
| UA35611C2 (en) | Topical composition for inhibiting hair growth in mammals, method for application and topical composition for delivering water-soluble pharmaceutical to skin | |
| PL335979A1 (en) | Antitussive compositions | |
| PL339663A1 (en) | Application of at least one axtract from plants belonging to the chrysanthemum genus to facilitate pigmentation of skin and/or hair | |
| AU2012216795B2 (en) | Soy depigmenting and skin care compositions | |
| HUP9902415A2 (en) | Solid instant-release forms of administration and process for producing the same | |
| WO2002055047A8 (en) | Skin whitening composition containing arbutin and glucosidase as active ingredients | |
| WO1997035597A1 (en) | Acne treatment | |
| GEP20022751B (en) | Aqueous Pharmaceutical Composition Comprising an Active Ingredient Which Is Highly Insoluble in Water | |
| AU643760B2 (en) | Method and composition for increased skin concentration of active agents by iontophoresis | |
| GB1474424A (en) | Pharmaceutical composition for treating diabetic ketoacidosis | |
| JPH01242516A (en) | Hair tonic, hair shampoo and hair growing promoter | |
| AU749923C (en) | The use of combinations of active agents consisting of antimicrobially active substances and plant extracts containing terpene in veterinary medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |