AU2020291943B2

AU2020291943B2 - Compositions, kits, methods and uses for preventing microbial growth

Info

Publication number: AU2020291943B2
Application number: AU2020291943A
Authority: AU
Inventors: Hojabr Alimi; Sridhar Govinda PRASAD; Santosh C. Sinha
Original assignee: Collidion Inc
Current assignee: Collidion Inc
Priority date: 2019-06-14
Filing date: 2020-06-14
Publication date: 2026-02-19
Anticipated expiration: 2040-06-14
Also published as: JP2026021384A; IL288856B2; WO2020252433A1; EP3982735A4; US20220323626A1; IL288856B1; AU2020291943A1; IL288856A; CN114269159A; JP2022536927A; CA3143270A1; EP3982735A1; MX2021015432A; ZA202200652B; KR20220054575A; BR112021025195A2

Abstract

The present specification discloses a composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available chlorine alone or in combination with one or more quaternary compounds or silicon quaternary compounds, one or more guanide-containing compounds, one or more alcohols, one or more metallic particles, one or more metal salts, or any combination thereof. The present specification further discloses a kit comprising, consisting essentially of, or consisting of a one or more containers including a composition disclosed herein or components which make up such compositions as well as methods and uses for such compositions and kits.

Description

Compositions, Kits, Methods and Uses for Preventing Microbial Growth

[001] This application claims the benefit of priority and is entitled to the filing date pursuant to 35 U.S.C.

§ 119(e) of U.S. Provisional Patent Application 62/861,987 filed June 14, 2019, and U.S. Provisional Patent

Application 62/977,090, filed February 14, 2020, the content of each of which is hereby incorporated by

reference in its entirety.

[002] Water plays an important role in human health and also plays an important role in the health care

field as well as in a variety of residential, commercial and industrial settings. Water or other liquid sources

may become contaminated with organic materials and various other compounds that support microbial

growth and residual microbes, including bacteria, algae, fungi (including mold, mildew, and yeast), and

viruses. Control and prevention of microbial growth within such water or other liquid sources itself is a

critical aspect in a wide range of industries and uses. Non-sterile water is most often used within medical

devices operated at home or hospital (I.s. CPAP). As such, it is of vital importance for many individuals,

especially those with weakened immune systems or disease, that non-sterile water when used does not

promote an environment for further bacterial propagation leading to higher chances of infection for patient.

[003] For example, in the healthcare industry the use of sterile and non-sterile water or another liquid

free of pathogenic levels of microbes is needed in the manufacture and/or delivery of pharmaceutical drugs

and other regulated and non-regulated products for internal or external use by an individual as well as in

the operation and/or performance of medical devices, equipment, machinery or other apparatus Although

medical devices, equipment, and machinery are themselves sterilized or disinfected prior to use, the water

or other liquid added to these devices, equipment, or machinery after such cleaning treatment may either

introduce microbes or subsequently become contaminated by many factors including but not limited to

patient use during device operation or separately during storage of the device. For instance, the use of

water within heart-lung bypass machines used in conducting open heart surgery is currently under

investigation for probable cause of primary infection in patients undergone such surgical procedure where

symptoms of such infection may take up to several months to reveal itself with mortality rate of greater than

40% in this patient population. Other medical devices which also rely on water as part of their operation

have similar contamination issues, such as, e.g., Continuous Positive Airway Pressure (CPAP). Further,

microbes can contaminate adjacent medical equipment and the patient environment in general including

surgical rooms. Hospital acquired infections (HAI) are a major cause of mortality and morbidity and

represent a significant cost to the healthcare system in the U.S. and globally. As such, the type and

certification of water or other liquid used in these medical applications are of high importance to FDA and

as such are highly controlled by this regulatory agency.

[004] Similarly, considering the apparatus, microbial growth can readily spread from contaminated water

or other liquid contained source into associated residential, commercial or industrial equipment, appliances

or machinery as well as a house, building or facility. Such apparatus include, without limitation,

refrigerators, freezers, dishwashers, clothes washers, water heaters, air conditioners, cooling towers, or

any other apparatus using water in the course of its operation. Thus, it is critically important to have water or other liquid free of pathogenic levels of microbes which can also prevent subsequent microbial growth that could arise from a contamination event during the storage or use of a device, equipment, machinery or other apparatus as well as a substance like a drug or food.

[005] Simply diluting a sterilizing agent or disinfectant to a concentration that restrains or prevents

microbial growth is not a viable solution. For example, chemical agents used to sterilize or disinfect a

device, such as a medical device tend to be either an irritant or toxic to an individual. As such, even in

lower concentrations such chemical agents are not useful in applications where an individual will be

exposed to water or another liquid containing these chemical agents. As another example, microbiocidal

agents, including antibiotics, used at lower concentrations act as a mutagen facilitating the development of

microbes that are resistant to these microbiocidal agents. Furthermore, microbiocidal agents in various

industries, like the agricultural industry or the paper manufacturing industry inevitably enter the waterways

caused adverse environmental impact as well as increasing the likelihood of creating resistant microbes.

[006] Microbiostatic water products are currently marketed as injectable additives for mixing with

pharmaceutical preparations that are then administered by intravenous, intramuscular, or subcutaneous

injection. Such preparations use lower levels of microbiostatic agents to safely prevent adverse levels of

bacterial contamination within the injectable prior to patient exposure. One example of these products may

include 0.9% benzyl alcohol in combination with sterile water. However, benzyl alcohol has been

associated with toxicity and hypersensitivity in some individuals and may result in local reactions and

histamine release. In cases where patients are unable to metabolize benzyl alcohols properly, results can

be fatal with prolonged exposure.

[007] Microbiostatic agents are also added to water to prevent microbial contamination in home

humidifiers. The agents used in these solutions can include alkyl dimethyl and diethyl ammonium chlorides.

These agents are known to damage mucosal and other human tissues. The use in home humidifiers

typically prevents the vaporization of the microbiostatic agent and is also typically used in general (room)

proximity to an individual. Medical applications using humidity, however, require the use be directly on or

in a patient, thus, microbiostatic compositions comprising alkyl dimethyl and diethyl ammonium chloride are

not suitable for such medical applications.

[008] Other microbiostatic agents can also be created using lower-than-effective, pre-existing anti-

microbial agents that are capable of killing bacteria and microbes, respectively, at therapeutic

concentrations. However, such formulation will partially expose microbes to low concentrations of the anti-

microbial agents. Historically, antimicrobial resistance (AMR) is created, in part, through overuse, misuse,

prolonged use of prophylactic anti-microbial products. As microbes are exposed to therapeutic agents, they

build resistance to the anti-microbial agents. One mechanism of such adaptation would be survival of strains

of microbes that naturally develop resistant mechanisms through their own rapid multiplication and genetic

diversity. Through a natural selection process, survivability is optimized with greater exposure.

[009] As such, there is a need to develop safe, non-toxic compositions that can either substitute for or

be added to water or another liquid that restrain, prohibit, or otherwise prevents microbial population growth.

The present specification discloses compositions, methods and uses that provide superior prevention of

microbial population growth in water or other liquid. The water or other liquid can be manufactured as

sterile or non-sterile. A disclosed composition can be added to water or other liquid or substitute for these

fluids and can be used prior to or during an application. In addition to being effective, the discloses

compositions, methods and uses are safe and non-toxic to an individual and the environment and can be

used in any application where the disclosed compositions are used directly on or in an individual or will be

exposed to the environment. Furthermore, the likelihood of development of antibiotic resistance bacteria

and other microbes is greatly reduced as the discloses compositions, methods and uses are not mutagenic

to the microbes and have not been shown to induce antimicrobial resistance. In addition, in preventing

microbial population growth in water or other liquid, the disclosed compositions, methods and uses reduce

and prevent subsequent microbial contamination to other individuals or devices exposed to the treated

water or other liquid. A disclosed composition provides a unique product that is shelf-stable and safe for

individuals who using microbiostatic water-containing solutions.

SUMMARY

[010] Aspects of the present specification disclose a composition comprising one or more microbiostatic

agents. The amount of one or more microbiostatic agents can be an amount that restrains or inhibits

microbial population growth. A microbiostatic agent comprises hypochlorous acid or free available chlorine,

one or more guanide-containing compounds, one or more surfactants, one or more quaternary compound

or silicon quaternary compounds, one or more metallic particles, one or more metal salts and/or a

phosphate buffer like calcium phosphate or sodium phosphate. A composition disclosed herein is stable

and is effective under environmental conditions that promote microbial growth.

[011] Aspects of the present specification disclose a composition comprising hypochlorous acid or free

available chlorine. The amount of hypochlorous acid or free available chlorine can be an amount that

restrains, prohibits, or otherwise prevents microbial population growth. A composition disclosed herein can

further comprise one or more guanide-containing compounds, one or more surfactants, one or more

quaternary compound or silicon quaternary compounds, one or more metallic particles, one or more metal

salts and/or a phosphate buffer like calcium phosphate or sodium phosphate.

[012] Aspects of the present specification disclose a composition comprising hypochlorous acid or free

available chlorine and one or more quaternary compounds or silicon quaternary compounds. The amount

of hypochlorous acid or free available chlorine and/or one or more quaternary compound or silicon

quaternary compounds can be an amount that restrains, prohibits, or otherwise prevents microbial

population growth. A composition disclosed herein can further comprise one or more guanide-containing

compounds, one or more surfactants, one or more metallic particles, one or more metal salts and/or a

phosphate buffer like calcium phosphate or sodium phosphate.

[013] Other aspects of the present specification disclose a composition comprising hypochlorous acid or

free available chlorine and one or more guanide-containing compounds. The amount of hypochlorous acid

or free available chlorine and/or one or more guanide-containing compounds can be an amount that,

prohibits, or otherwise prevents microbial population growth. A disclosed guanide-containing compound

includes an organic compound containing a biguanide functional group, a biguanidine functional group, a

triguanide functional group, or any combination thereof. A composition disclosed herein can further

comprise one or more quaternary compound or silicon quaternary compounds, one or more surfactants,

one or more metallic particles, one or more metal salts and/or a phosphate buffer like calcium phosphate

or sodium phosphate.

[014] Aspects of the present specification disclose a composition comprising hypochlorous acid or free

available chlorine and one or more guanide-containing compounds, and one or more surfactants. The

amount of hypochlorous acid or free available chlorine and/or one or more guanide-containing compounds

and/or one or more surfactants can be an amount that restrains, prohibits, or otherwise prevents microbial

population growth. A disclosed guanide-containing compound includes an organic compound containing a

biguanide functional group, a biguanidine functional group, a triguanide functional group, or any

combination thereof. A composition disclosed herein can further comprise one or more quaternary

compound or silicon quaternary compounds, one or more metallic particles, one or more metal salts and/or

a phosphate buffer like calcium phosphate or sodium phosphate.

[015] Other aspects of the present specification disclose a composition comprising one or more alcohols

and one or more quaternary compound or silicon quaternary compounds. The amount of one or more

alcohols and/or one or more quaternary compound or silicon quaternary compounds can be an amount that

restrains, prohibits, or otherwise prevents microbial population growth. A disclosed alcohol includes

ethanol, methanol, isopropyl alcohol, or any combination thereof. A composition disclosed herein can

metallic particles, one or more metal salts and/or a phosphate buffer like calcium phosphate or sodium

phosphate.

[016] Other aspects of the present specification disclose a composition comprising one or more alcohols

compound and one or more guanide-containing compounds. The amount of one or more alcohols and/or

one or more guanide-containing compounds can be an amount that restrains, prohibits, or otherwise

prevents microbial population growth. A disclosed alcohol includes ethanol, methanol, isopropyl alcohol,

or any combination thereof. A disclosed guanide-containing compound includes an organic compound

containing a biguanide functional group, a biguanidine functional group, a triguanide functional group, or

any combination thereof. A composition disclosed herein can further comprise one or more quaternary

compound or silicon quaternary compounds, one or more surfactants, one or more metallic particles, one

or more metal salts and/or a phosphate buffer like calcium phosphate or sodium phosphate.

[017] Other aspects of the present specification disclose a composition comprising hypochlorous acid or

free available chlorine and one or more metallic particles. The amount of hypochlorous acid or free available chlorine and/or one or more metallic particles can be an amount that restrains, prohibits, or otherwise prevents microbial population growth. A composition disclosed herein can further comprise one or more metal salts and/or a phosphate buffer like calcium phosphate or sodium phosphate.

[018] Other aspects of the present specification disclose a composition comprising hypochlorous acid or

free available chlorine and one or more metal salts. The amount of hypochlorous acid or free available

chlorine and/or one or more metallic salts can be an amount that restrains, prohibits, or otherwise prevents

microbial population growth. A composition disclosed herein can further comprise one or more metallic

particles and/or a phosphate buffer like calcium phosphate or sodium phosphate.

[019] Other aspects of the present specification disclose a kit comprising one or more containers

including a composition disclosed herein and/or one or more containers including components of a

composition disclosed herein. A disclosed kit further comprises one or more delivery or application

systems, and/or instructions, and/or a container.

[020] Other aspects of the present specification disclose a making a microbiostatic water or other liquid.

In aspects, a disclosed method comprises applying a microbiostatic agent disclosed herein to water or other

liquid in a microbiostatic effective amount, wherein application of the composition reduces or inhibits

microbial growth. In other aspects, a disclosed method comprises applying a microbiostatic agent disclosed

herein to water or other liquid in an amount that restrains, prohibits, or otherwise prevents microbial

population growth without free ting the microbes, wherein application of the composition reduces or inhibits

microbial growth. In yet other aspects, a disclosed method comprises applying a microbiostatic agent

disclosed herein to water or other liquid in an amount that restrains, prohibits, or otherwise prevents

microbial population growth without eradicating the microbes to a degree that achieves disinfection or

sterilization, wherein application of the composition reduces or inhibits microbial growth.

[021] Other aspects of the present specification disclose a method of reducing or inhibiting microbial

growth in water or other liquid. In aspects, a disclosed method comprises applying a composition disclosed

population growth without eradicating the microbes, wherein application of the composition reduces or

inhibits microbial growth. The water or other liquid can be manufactured as sterile or non-sterile. In other

aspects, a disclosed method comprises applying a composition disclosed herein to water or other liquid in

an amount that restrains, prohibits, or otherwise prevents microbial population growth without eradicating

the microbes to a degree that achieves disinfection or sterilization, wherein application of the composition

reduces or inhibits microbial growth. In yet other aspects of the present specification disclose a composition

disclosed herein for use in reducing or inhibiting microbial growth in water or other liquid. In still other

aspects of the present specification disclose a use of a disclosed composition to reduce or inhibit microbial

growth in water or other liquid.

[022] Other aspects of the present specification disclose a method of reducing or inhibiting microbial

growth in a medical device. In aspects, a disclosed method comprises applying a composition disclosed

PCT/US2020/037665 6

herein to water or other liquid present in a medical device in an amount that restrains microbial growth,

wherein application of the composition reduces or inhibits microbial growth in the water or other liquid. In

other aspects, a disclosed method comprises applying a composition disclosed herein to water or other

liquid present in a medical device in an amount that restrains, prohibits, or otherwise prevents microbial

population growth without eradicating the microbes to a degree that achieves disinfection or sterilization,

yet other aspects, a disclosed method comprises applying a composition disclosed herein to a medical

device, wherein application of the composition reduces or inhibits microbial growth prior and during

operation of the device. In still other aspects of the present specification disclose a composition disclosed

herein for use in reducing or inhibiting microbial growth in water or other liquid present in a medical device.

In other aspects of the present specification disclose a use of a disclosed composition to reduce or inhibit

microbial growth in a medical device.

[023] Other aspects of the present specification disclose a method to wash or irrigate a surface area. In

aspects a disclosed method comprises applying a composition disclosed herein to a surface area, wherein

application of the composition washes or irrigates the surface area. In other aspects of the present

specification disclose a composition disclosed herein for use in washing or irrigating a surface area. In yet

other aspects of the present specification disclose a use of a disclosed composition to wash or irrigate a

surface area.

[024] Other aspects of the present specification disclose a method to wash or irrigate a biological surface,

a tissue, a skin, or a body cavity. In aspects a disclosed method comprises applying a composition

disclosed herein to a surface area, wherein application of the composition washes or irrigates a biological

surface, a tissue, a skin, or a body cavity. In other aspects of the present specification disclose a

composition disclosed herein for use in washing or irrigating a biological surface, a tissue, a skin, or a body

cavity. In yet other aspects of the present specification disclose a use of a disclosed composition to wash

or irrigate a biological surface, a tissue, a skin, or a body cavity.

[025] Other aspects of the present specification disclose a method to wash or irrigate a wound in an

individual. In aspects, a disclosed method comprises applying a composition disclosed herein to the wound

of an individual, wherein application of the composition washes or irrigates the wound. In other aspects of

the present specification disclose a composition disclosed herein for use in washing or irrigating a wound

in an individual. In yet other aspects of the present specification disclose a use of a disclosed composition

to wash or irrigate a wound in an individual. In still other aspects of the present specification disclose a use

of a disclosed composition in the manufacture of a medicament to wash or irrigate a wound in an individual.

[026] Other aspects of the present specification disclose a method to treat and/or provide relief from a

wound in an individual. In aspects a disclosed method comprising applying a composition disclosed herein

to an individual, wherein application of the composition promotes healing of a wound. In other aspects of

the present specification disclose a composition disclosed herein for use in treating and/or providing relief

of a wound in an individual. In yet other aspects of the present specification disclose a use of a disclosed

WO wo 2020/252433 PCT/US2020/037665 7

composition to treat and/or provide relief from a wound in an individual. In still other aspects of the present

specification disclose a use of a disclosed composition in the manufacture of a medicament to treat and/or

provide relief from a wound in an individual.

[027] Other aspects of the present specification disclose a method to wash or irrigate an infection in an

individual. In aspects, a disclosed method comprising applying a composition disclosed herein to an

individual, wherein application of the composition washes or irrigates the infection. In other aspects of the

present specification disclose a composition disclosed herein for use in washing or irrigating an infection in

an individual. In yet other aspects of the present specification disclose a use of a disclosed composition to

wash or irrigate an infection in an individual. In still other of the present specification disclose a use of a

disclosed composition in the manufacture of a medicament to wash or irrigate an infection in an individual.

An infection can be a microbial infection.

[028] Other aspects of the present specification disclose a method to treat and/or provide relief from an

infection in an individual. In aspects a disclosed method comprising applying a composition disclosed

herein to an individual, wherein application of the composition promotes healing of an infection. In other

aspects of the present specification disclose a composition disclosed herein for use in treating and/or

providing relief of an infection in an individual. In yet other aspects of the present specification disclose a

use of a disclosed composition to treat and/or provide relief from an infection in an individual. In still other

aspects of the present specification disclose a use of a disclosed composition in the manufacture of a

medicament to treat and/or provide relief from an infection in an individual.

[029] Other aspects of the present specification disclose a method to wash or irrigate an inflammation in

an individual. In aspects, a disclosed method comprising applying a composition disclosed herein to an

individual, wherein application of the composition washes or irrigates the inflammation. In other aspects of

the present specification disclose a composition disclosed herein for use in washing or irrigating an

inflammation in an individual. In yet other aspects of the present specification disclose a use of a disclosed

composition to wash or irrigate an inflammation in an individual. In still other aspects of the present

specification disclose a use of a disclosed composition in the manufacture of a medicament to wash or

irrigate an inflammation in an individual.

[030] Other aspects of the present specification disclose a method to treat and/or provide relief from an

inflammation in an individual. In aspects a disclosed method comprising applying a composition disclosed

herein to an individual, wherein application of the composition promotes healing of an inflammation. In

other aspects of the present specification disclose a composition disclosed herein for use in treating and/or

providing relief of an inflammation in an individual. In yet other aspects of the present specification disclose

a use of a disclosed composition to treat and/or provide relief from an inflammation in an individual. In still

other aspects of the present specification disclose a use of a disclosed composition in the manufacture of

a medicament to treat and/or provide relief from an inflammation in an individual.

WO wo 2020/252433 PCT/US2020/037665 8

DETAILED DESCRIPTION

[031] A composition disclosed herein comprises one or more microbiostatic agents that reduces or

prevents growth of microbes. Microbiostatic agents are biological or chemical agents that directly or

indirectly restrain, prohibit or otherwise prevent growth of microbes for given time period. Exposure to a

microbiostatic agent disclosed herein can be reversed such that microbial growth could resume once the

microbiostatic agent is removed. In addition, when added to water or other fluids, a microbiostatic agent

disclosed herein is not designed to disinfect, sterilize or otherwise eliminate microbial contamination.

Furthermore, microbiostatic agents classified by regulatory agencies including EPA and FDA and they

require distinct testing protocols that are vastly different from disinfection and sterilization test assays

adopted by the same regulatory agencies. As such, a microbiostatic agent disclosed herein is not a

microbiocidal agent because it does not necessarily irreversibly eradicate microbes. A microbiostatic agent

disclosed herein includes, without limitation, hypochlorous acid, free available chlorine, one or more

guanide-containing compounds, one or more surfactants, one or more quaternary compound or silicon

quaternary compounds, one or more metallic particles and/or one or more metal salts. A microbe, includes,

without limitation, a bacterium, an alga, a fungus (including a mold, a mildew, and a yeast), and a virus.

[032] A composition disclosed herein restrains, prohibits or otherwise prevents growth of populations of

microbes for given time period. Exposure to a composition disclosed herein can be reversed such that

microbial growth could resume once the composition is removed. When used as a substitute for water or

other liquid, a composition disclosed herein is not designed to disinfect, sterilize or otherwise eliminate

microbial contamination on a surface, or in a device, equipment, machinery or other apparatus. When

added to water or other liquid, a composition disclosed herein is not designed to disinfect, sterilize or

otherwise eliminate microbial contamination either in the water or water-based solution, or a surface, or a

device, equipment, machinery or other apparatus. As such, a composition disclosed herein is a

microbiostatic composition and not a microbiocidal composition.

[033] Without wishing to be limited by theory, a composition disclosed herein directly or indirectly

restrains, prohibits or otherwise prevents microbial growth by, e.g., preventing cell division or an aspect

thereof, such as karyokinesis or cytokinesis. For example, a composition disclosed herein can directly

restrain, inhibit or otherwise prevent an aspect of cell division by, e.g., inactivating an enzyme or other

component needed for an aspect of cell division. As another example, a composition disclosed herein can

indirectly restrain, inhibit or otherwise prevent an aspect of cell division by, e.g., sequestering a metabolite

or nutrient needed for microbial health (unhealthy microbes will not reproduce) or an aspect of cell division.

As such, a composition disclosed herein is a broad-spectrum microbiostatic composition that restrains,

prohibits, or otherwise prevents microbial population growth without eradicating the microbes. In an aspect

of this embodiment, a composition disclosed herein is a broad-spectrum microbiostatic composition that

restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbes to

a degree that achieves disinfection or sterilization.

[034] A disclosed composition can be formulated to be used directly as an undiluted composition, e.g.,

in a method disclosed herein. Such a composition is formulated with a microbiostatic effective amount of

one or more microbiostatic agents. In addition, a disclosed composition can be formulated as a concentrate

and used in a diluted form or as an additive to a water or another fluid, e.g., in a method disclosed herein.

Such a composition is formulated with one or more microbiostatic agents in a manner that the final

concentration of one or more microbiostatic agents used in an application will be a microbiostatic effective

amount. A composition disclosed herein can be manufactured under sterile conditions and/or subject to

subsequent sterilization procedure, such as, e.g., filtration.

[035] A composition disclosed herein contains a microbiostatic effective amount of a microbiostatic agent.

A microbiostatic effective amount is an amount that restrains, prohibits or otherwise prevents growth of

populations of microbes for given time period. Microbial growth or microbial population growth refers to a

process whereby microbes multiply or reproduce themselves to create new microbes by, e.g., cell division,

sporation, or replication. As such, a microbiostatic effective amount directly or indirectly prevents microbes

from undergoing a process that multiplies or reproduces themselves by, e.g., preventing cell division or an

aspect thereof, such as karyokinesis or cytokinesis, but does not eradicate these microbes. In an aspect

of this embodiment, a microbiostatic effective amount is an amount that restrains, prohibits or otherwise

prevents growth of populations of microbes for given time period without entirely eradicating the microbes.

In another aspect of this embodiment, a microbiostatic effective amount is an amount that restrains,

prohibits or otherwise prevents growth of populations of microbes for given time period without eradicating

the microbes to a degree that achieves disinfection or sterilization. In yet another aspect of this

embodiment, a microbiostatic effective amount includes a microbial sub-lethal amount (also referred to as

a microbial non-lethal amount), or a minimum inhibitory concentration (MIC).

[036] In aspects of this embodiment, a microbiostatic effective amount can be a bacteriostatic effective

amount, an algaestatic effective amount, a fungistatic effective amount and/or a virustatic effective amount.

A bacteriostatic effective amount is an amount that restrains bacterial growth for given time period without

eradicating the bacteria. In an aspect of this embodiment, a bacteriostatic effective amount is an amount

that restrains bacterial growth for given time period without eradicating the bacteria to a degree that

achieves disinfection or sterilization. An algeastatic effective amount is an amount that restrains algal

growth for given time period without eradicating the algae to a degree that achieves disinfection or

sterilization. A fungistatic effective amount is an amount that restrains fungal growth for given time period

without eradicating the fungi. In an aspect of this embodiment, a fungistatic effective amount is an amount

that restrains fungal growth for given time period without eradicating the fungi to a degree that achieves

disinfection or sterilization. A virustatic effective amount is an amount that restrains viral replication for

given time period without eradicating the virus to a degree that achieves disinfection or sterilization. In an

aspect of this embodiment, a virustatic effective amount is an amount that restrains viral replication for

given time period without eradicating the virus to a degree that achieves disinfection or sterilization.

[037] In aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent disclosed

herein is an amount that 1) restrains, prohibits or otherwise prevents growth of a microbial population; 2)

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results in no more than a 1 X 102 reduction in the microbial population within the first seven days of

continuous exposure to the microbiostatic agent; and 3) results in a minimum of a 1 X 102 reduction in the

microbial population after at least 14 days of continuous exposure to the microbiostatic agent without

achieving full kill of the microbial population. In other aspects of this embodiment, a microbiostatic effective

amount of a microbiostatic agent disclosed herein is an amount that 1) restrains, prohibits or otherwise

prevents growth of a microbial population; 2) results in no more than a 1 X 102 reduction in the microbial

population within the first seven days of continuous exposure to the microbiostatic agent; and 3) results in

a minimum of a X 102 reduction in the microbial population after at least 14 days of continuous exposure

to the microbiostatic agent but no more than 1 X 104 reduction of the microbial population, no more than 1

X 105 reduction of the microbial population, no more than 1 X 106 reduction of the microbial population, or

no more than 1 X 107 reduction of the microbial population.

[038] In aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent disclosed

results in about 1 X 10° to about x 10 ² reduction in the microbial population within the first seven days of

prevents growth of a microbial population; 2) results in about 1 X 10° to about 1 X 102 reduction in the

microbial population within the first seven days of continuous exposure to the microbiostatic agent; and 3)

results in a minimum of a 1 X 102 reduction in the microbial population after at least 14 days of continuous

exposure to the microbiostatic agent but no more than 104 reduction of the microbial population, no

more than 1 X 105 reduction of the microbial population, no more than 1 X 106 reduction of the microbial

population, or no more than 1 X 107 reduction of the microbial population.

[039] In aspects of this embodiment, a sub-lethal amount of a microbiostatic agent disclosed herein is an

amount that 1) restrains, prohibits or otherwise prevents growth of a microbial population; 2) results in no

more than a X 102 reduction in the microbial population within the first seven days of continuous exposure

to the microbiostatic agent; and 3) results in a minimum of a X 102 reduction in the microbial population

after at least 14 days of continuous exposure to the microbiostatic agent without achieving full kill of the

microbial population. In other aspects of this embodiment, a sub-lethal amount of a microbiostatic agent

disclosed herein is an amount that 1) restrains, prohibits or otherwise prevents growth of a microbial

population; 2) results in no more than a 1 102 reduction in the microbial population within the first seven

days of continuous exposure to the microbiostatic agent; and 3) results in a minimum of a 1 X 102 reduction

in the microbial population after at least 14 days of continuous exposure to the microbiostatic agent but no

more than 1 X 104 reduction of the microbial population, no more than 1 X 105 reduction of the microbial

population, no more than 1 X 106 reduction of the microbial population, or no more than 1 X 107 reduction

of the microbial population.

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[040] In aspects of this embodiment, a sub-lethal amount of a microbiostatic agent disclosed herein is an

amount that 1) restrains, prohibits or otherwise prevents growth of a microbial population; 2) results in about

1 X 10° to about 1 X 102 reduction in the microbial population within the first seven days of continuous

exposure to the microbiostatic agent; and 3) results in a minimum of a 1 X 102 reduction in the microbial

population after at least 14 days of continuous exposure to the microbiostatic agent without achieving full

kill of the microbial population. In other aspects of this embodiment, a sub-lethal amount of microbiostatic

agent disclosed herein is an amount that 1) restrains, prohibits or otherwise prevents growth of a microbial

population; 2) results in about 1 X 10° to about 1 X 102 reduction in the microbial population within the first

seven days of continuous exposure to the microbiostatic agent; and 3) results in a minimum of a X 102

reduction in the microbial population after at least 14 days of continuous exposure to the microbiostatic

agent but no more than 1 X 104 reduction of the microbial population, no more than 1 X 105 reduction of the

microbial population, no more than 1 X 106 reduction of the microbial population, or no more than X 107

reduction of the microbial population.

[041] A minimum microbiocidal concentration is the lowest amount of a microbiostatic agent disclosed

herein or a composition disclosed herein that will eradicate the microbes comprising a microbial population

within one day. In aspects of this embodiment, a minimum microbiocidal concentration of a microbiostatic

agent disclosed herein or a composition disclosed herein achieves in one day, e.g., more than a 1 X 104

reduction of the microbial population, more than a X 105 reduction of the microbial population, more than

a X 106 reduction of the microbial population, or more than a 1 x 10 reduction of the microbial population.

In an embodiment, a composition disclosed herein is formulated with one or more microbiostatic agents

disclosed herein in an amount that is not a minimum microbiocidal concentration. In an embodiment, a

composition disclosed herein is formulated with one or more microbiostatic agents disclosed herein in an

amount that is below the minimum microbiocidal concentration of the one or more microbiostatic agents.

In another embodiment, the amount of one or more microbiostatic agents disclosed herein or a composition

disclosed herein used in a method disclosed herein is an amount that is not a minimum microbiocidal

concentration of the one or more microbiostatic agents or the composition. In another embodiment, the

amount of one or more microbiostatic agents disclosed herein or a composition disclosed herein used in a

method disclosed herein is an amount below the minimum microbiocidal concentration of the one or more

microbiostatic agents or the composition.

[042] In an embodiment, a microbiostatic effective amount of a microbiostatic agent disclosed herein is

an amount that restrains, prohibits or otherwise prevents growth of populations of microbes for a given

period of time. In aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent

disclosed herein is an amount that restrains, prohibits or otherwise prevents growth of populations of

microbes for, e.g., about 4 hours, about 8 hours, about 12 hours, about 16 hours, about 20 hours or about

24 hours. In other aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent

microbes for, e.g., at least 4 hours, at least 8 hours, at least 12 hours, at least 16 hours, at least 20 hours

or at least 24 hours. In yet other aspects of this embodiment, a microbiostatic effective amount of a

microbiostatic agent disclosed herein is an amount that restrains, prohibits or otherwise prevents growth of populations of microbes for, e.g., at most 4 hours, at most 8 hours, at most 12 hours, at most 16 hours, at most 20 hours or at most 24 hours. In still other aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent disclosed herein is an amount that restrains, prohibits or otherwise prevents growth of populations of microbes for, e.g., about 4 hours to about 8 hours, about 4 hours to about

12 hours, about 4 hours to about 16 hours, about 4 hours to about 20 hours, about 4 hours to about 24

hours, about 8 hours to about 12 hours, about 8 hours to about 16 hours, about 8 hours to about 20 hours,

about 8 hours to about 24 hours, about 12 hours to about 16 hours, about 12 hours to about 20 hours,

about 12 hours to about 24 hours, about 16 hours to about 20 hours, about 16 hours to about 24 hours, or

about 20 hours to about 24 hours.

[043] In other aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent

microbes for e.g., about 1 day, about 2 days, 3 days, 4 days, 5 days, 6, days, 7 days, 8 days, 9 days, 10

days, 11 days, 12 days, 13 days, 14 days, 15 days, about 20 days, about 25 days, or about 30 days. In yet

other aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent disclosed

herein is an amount that restrains, prohibits or otherwise prevents growth of populations of microbes for,

e.g., at least 1 day, at least 2 days, at least 3 days, at least 4 days, at least 5 days, at least 6, days, at least

7 days, at least 8 days, at least 9 days, at least 10 days, at least 11 days, at least 12 days, at least 13 days,

at least 14 days, at least 15 days, at least 20 days, at least 25 days, or at least 30 days. In still other

aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent disclosed herein is

an amount that restrains, prohibits or otherwise prevents growth of populations of microbes for, e.g., at

most 1 day, at most 2 days, at most 3 days, at most 4 days, at most 5 days, at most 6, days, at most 7

days, at most 8 days, at most 9 days, at most 10 days, at most 11 days, at most 12 days, at most 13 days,

at most 14 days, at most 15 days, at most 20 days, at most 25 days, or at most 30 days.

[044] In other aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent

microbes for, e.g., about 1 day to about 2 days, about 1 day to about 3 days, about 1 day to about 4 days,

about 1 day to about 5 days, about 1 day to about 6 days, about 1 day to about 7 days, about 1 day to

about 8 days, about 1 day to about 9 days, about 1 day to about 10 days, about 1 day to about 11 days,

about 1 day to about 12 days, about 1 day to about 13 days, about 1 day to about 14 days, about 1 day to

about 15 days, about 1 day to about 20 days, about 1 day to about 25 days, about 1 day to about 30 days,

about 2 days to about 3 days, about 2 days to about 4 days, about 2 days to about 5 days, about 2 days to

about 6 days, about 2 days to about 7 days, about 2 days to about 8 days, about 2 days to about 9 days,

about 2 days to about 10 days, about 2 days to about 11 days, about 2 days to about 12 days, about 2 days

to about 13 days, about 2 days to about 14 days, about 2 days to about 15 days, about 2 days to about 20

days, about 2 days to about 25 days, about 2 days to about 30 days, about 3 days to about 4 days, about

3 days to about 5 days, about 3 days to about 6 days, about 3 days to about 7 days, about 3 days to about

8 days, about 3 days to about 9 days, about 3 days to about 10 days, about 3 days to about 11 days, about

3 days to about 12 days, about 3 days to about 13 days, about 3 days to about 14 days, about 3 days to

about 15 days, about 3 days to about 20 days, about 3 days to about 25 days, about 3 days to about 30

PCT/US2020/037665 13

days, about 4 days to about 5 days, about 4 days to about 6 days, about 4 days to about 7 days, about 4

days to about 8 days, about 4 days to about 9 days, about 4 days to about 10 days, about 4 days to about

11 days, about 4 days to about 12 days, about 4 days to about 13 days, about 4 days to about 14 days,

about 4 days to about 15 days, about 4 days to about 20 days, about 4 days to about 25 days, about 4 days

to about 30 days, about 5 days to about 6 days, about 5 days to about 7 days, about 5 days to about 8

days, about 5 days to about 9 days, about 5 days to about 10 days, about 5 days to about 11 days, about

5 days to about 12 days, about 5 days to about 13 days, about 5 days to about 14 days, about 5 days to

about 15 days, about 5 days to about 20 days, about 5 days to about 25 days, about 5 days to about 30

days, about 6 days to about 7 days, about 6 days to about 8 days, about 6 days to about 9 days, about 6

days to about 10 days, about 6 days to about 11 days, about 6 days to about 12 days, about 6 days to

about 13 days, about 6 days to about 14 days, about 6 days to about 15 days, about 6 days to about 20

days, about 6 days to about 25 days, about 6 days to about 30 days, about 7 days to about 8 days, about

7 days to about 9 days, about 7 days to about 10 days, about 7 days to about 11 days, about 7 days to

about 12 days, about 7 days to about 13 days, about 7 days to about 14 days, about 7 days to about 15

days, about 7 days to about 20 days, about 7 days to about 25 days, about 7 days to about 30 days, about

8 days to about 9 days, about 8 days to about 10 days, about 8 days to about 11 days, about 8 days to

about 12 days, about 8 days to about 13 days, about 8 days to about 14 days, about 8 days to about 15

days, about 8 days to about 20 days, about 8 days to about 25 days, about 8 days to about 30 days, about

9 days to about 10 days, about 9 days to about 11 days, about 9 days to about 12 days, about 9 days to

about 13 days, about 9 days to about 14 days, about 9 days to about 15 days, about 9 days to about 20

days, about 9 days to about 25 days, about 9 days to about 30 days, about 10 days to about 11 days, about

10 days to about 12 days, about 10 days to about 13 days, about 10 days to about 14 days, about 10 days

to about 15 days, about 10 days to about 20 days, about 10 days to about 25 days, about 10 days to about

30 days, about 11 days to about 12 days, about 11 days to about 13 days, about 11 days to about 14 days,

about 11 days to about 15 days, about 11 days to about 20 days, about 11 days to about 25 days, about

11 days to about 30 days, about 12 days to about 13 days, about 12 days to about 14 days, about 12 days

to about 15 days, about 12 days to about 20 days, about 12 days to about 25 days, about 12 days to about

30 days, about 13 days to about 14 days, about 13 days to about 15 days, about 13 days to about 20 days,

about 13 days to about 25 days, about 13 days to about 30 days, about 14 days to about 15 days, about

14 days to about 20 days, about 14 days to about 25 days, about 14 days to about 30 days, about 15 days

to about 20 days, about 15 days to about 25 days, about 15 days to about 30 days, about 20 days to about

25 days, about 20 days to about 30 days, or about 25 days to about 30 days.

[045] In other aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent

microbes for, e.g., about 1 week, about 2 weeks, 3 weeks, or 4 weeks. In yet other aspects of this

embodiment, a microbiostatic effective amount of a microbiostatic agent disclosed herein is an amount that

restrains, prohibits or otherwise prevents growth of populations of microbes for, e.g., at least 1 week, at

least 2 weeks, at least 3 weeks, or at least 4 weeks. In still other aspects of this embodiment, a

microbiostatic effective amount of a microbiostatic agent disclosed herein is an amount that restrains,

prohibits or otherwise prevents growth of populations of microbes for, e.g., at most 1 week, at most 2 weeks, at most 3 weeks, or at most 4 weeks. In aspects of this embodiment, a microbiostatic effective amount of a microbiostatic agent disclosed herein is an amount that restrains, prohibits or otherwise prevents growth of populations of microbes for, e.g., about 1 week to about 2 weeks, about 1 week to about 3 weeks, about

1 week to about 4 weeks, about 2 weeks to about 3 weeks, about 2 weeks to about 4 weeks, or about 3

weeks to about 4 weeks.

[046] A composition disclosed herein has increased stability and efficacy. In one embodiment, this

increased stability and efficacy is due to the protection of hypochlorous acid. In part, the composition may

protect hypochlorous acid from exposure to harmful agents, including, e.g., organic compounds, reactive

compounds, positively charged molecules, or other compounds that promote or facilitate degradation of

hypochlorous acid. For example, the disclosed compositions are stable in blood serum and in plastic

packaging material. In addition, a synergistic effect in efficacy is observed when hypochlorous acid is

protected against this exposure. This significantly improved efficacy allows for lower amounts of

hypochlorous acid to be used in the disclosed compositions. As concentrations of hypochlorous acid above

350 ppm, particularly above 500 ppm produces instability, the lower amounts of hypochlorous acid used in

the disclosed compositions increases stability of the composition even further. As such, a composition

disclosed herein is seen as a replacement to any and all prior hypochlorous acid compositions currently in

the market or being developed.

[047] The stability of a composition disclosed herein may be due to the positively charged N atom of a

quaternary compound or silicon quaternary compound, a metallic particle, a metal salt, a phosphate buffer

like calcium phosphate or sodium phosphate, and/or a guanide-containing compound disclosed herein.

Such compounds appear to form a strong ionic interaction with the negatively charged (OCI), and the

complex thus formed retains its stability over period of time compared to hypochlorous acid alone, which is

a weaker complex in solution relative to a composition disclosed herein. For example, in the presence of

copper chloride hypochlorous acid forms copper hypochlorite and HCI and in the presence of zinc chloride

hypochlorous acid forms zinc hypochlorite and HCI. By replacing sodium hydroxide with calcium oxide, a

less basic solution is formed because in the presence of hypochlorous acid calcium oxide forms calcium

hypochlorite and water. The reduced levels of base enhances the stability of copper hypochlorite and zinc

hypochlorite because there is less base to react with zinc and copper ions, thereby maintaining the stability

of copper hypochlorite and zinc hypochlorite. Further, the use of 500 ppm or lower, an in particular 350

ppm or lower of hypochlorous acid further increases the stability of a composition disclosed herein.

[048] The improved efficacy of a composition disclosed herein may in part be a result of chemical bond

formation of a quaternary compound or silicon quaternary compound, a metallic particle, a metal salt,

aphosphate buffer like calcium phosphate or sodium phosphate, and/or a guanide-containing compound

disclosed herein with the substrates thereby providing a long-lasting activity combined with the free OCI,

which has its own antimicrobial activity.

[049] A composition disclosed herein may comprise hypochlorous acid. A weak acid, the chemical

formula of hypochlorous acid is HOCI, while its molecular formula is written as HCIO. As shown in formula

I, hypochlorous acid is a simple molecule with the central oxygen connected to chlorine and hydrogen

atoms through single bonds and has molar mass is 52.46 g/mol.

CI (I) H

[050] Hypochlorous acid is a colorless solution, and its exact physical properties are variable, depending

on the concentration of hypochlorous in solution. Hypochlorous acid reacts with bases to form salts called

hypochlorites. For example, sodium hypochlorite (NaOCI), the active ingredient in bleach, is formed by

reacting hypochlorous acid with sodium hydroxide. Hypochlorous acid also readily reacts with a variety of

organic molecules and biomolecules.

[051] The hypochlorous acid solution can be produced, e.g., by dissolving chlorine in water, hydrolysis

of chlorine gas, electrolysis of a salt solution or acidification of hypochloride or sodium hypochloride. For

example, stable hypochlorous salts, such as, e.g., alkali metal hypochlorites like sodium hypochlorite,

calcium hypochlorite, potassium hypochlorite, lithium hypochlorite and magnesium hypochlorite, can be

obtained by dissolving chlorine gas into an aqueous alkali metal hydroxide solution, like a sodium hydroxide

solution, a calcium hydroxide solution, a potassium hydroxide solution, a lithium hydroxide solution, or a

magnesium hydroxide solution. Hypochlorous acid can also be prepared by dissolving dichlorine monoxide

in water. As another example, hypochlorous acid can also be produced by electrolytically treating a saline

solution. In one method, an electrical current is applied to a one-, two-, or three-compartment cell

comprising a cathode chamber, an anode chamber, and a central saline solution chamber interposed

between the other two chambers where each chamber is separated by a semi-permeable membrane.

During electrolysis, sodium chloride (NaCI) dissociates into negatively charged chloride (CI) and positively

charged sodium (Na+). At the same time, water dissociates into hydroxide (OH-) and hydrogen (H+) ions

are formed. The negatively charged chloride (CIT) and hydroxide (OHT) ions move to the anode to lose

electrons and form hypochlorous acid (HOCI) as well as hypochlorite ions (OCI) and oxygen (O2) and

chlorine (Cl2) gases. The reductive water comprising the hypochlorous acid is then dispensed into a

collection chamber for subsequent use. Methods to produce hypochlorous acid are described in, e.g., U.S.

Patent Nos 3,914,397 4,190,638, 4,908,215, 5,322,677, 6,426,066, 7,323,118, 8,062,500, 8,945,630,

9,168,318, and 9,486,479, each of which is hereby incorporated by reference in its entirety.

[052] In an embodiment, a composition disclosed herein comprises an amount of hypochlorous acid that

provides a desired beneficial effect to a composition disclosed herein. For example, when a composition

comprising hypochlorous acid is formulated to be used in an undiluted state, the amount of hypochlorous

acid used is a microbiostatic effective amount. Alternatively, when a composition comprising hypochlorous

acid is formulated to be used in methods where the composition will be diluted, the amount of hypochlorous

acid used in such concentrated formulations can be any amount that does not cause precipitation of

components in the composition disclosed herein or otherwise create unwanted effects that make the

composition inoperable.

WO wo 2020/252433 PCT/US2020/037665 16 16

[053] In aspects of this embodiment, a composition disclosed herein comprises hypochlorous acid in an

amount of, e.g., 0.01 ppm, 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm, 0.40

ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80 ppm, 0.85 ppm, 0.90

ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm, 50

ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm, 95 ppm, 100 ppm, 125 ppm,

150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm, 350 ppm, 375 ppm, 400

ppm, 425 ppm, 450 ppm, 475 ppm, 500 ppm, 525 ppm, 550 ppm, 575 ppm, 600 ppm, 625 ppm, 650 ppm,

675 ppm, 700 ppm, 725 ppm, 750 ppm, 775 ppm, 800 ppm, 825 ppm, 850 ppm, 875 ppm, 900 ppm, 925

ppm, 950 ppm, 975 ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm, 1075 ppm, 1,100 ppm, 1,125 ppm, 1,150

ppm, 1,175 ppm, 1,200 ppm, 1,225 ppm, 1,250 ppm, 1,275 ppm, 1,300 ppm, 1,325 ppm, 1,350 ppm, 1,375

ppm, 1,400 ppm, 1,425 ppm, 1,450 ppm, 1,475 ppm, 1,500 ppm, 1,600 ppm, 1,700 ppm, 1,800 ppm, 1,900

ppm, or 2,000 ppm. In other aspects of this embodiment, a composition disclosed herein comprises

hypochlorous acid in an amount of, e.g., at least 0.01 ppm, at least 0.05 ppm, at least 0.10 ppm, at least

0.20 ppm, at least 0.30 ppm, at least 0.40 ppm, at least 0.50 ppm, at least 0.60 ppm, at least 0.70 ppm, at

least 0.80 ppm, at least 0.90 ppm, at least 1 ppm, at least 10 ppm, at least 20 ppm, at least 30 ppm, at least

40 ppm, at least 50 ppm, at least 60 ppm, at least 70 ppm, at least 80 ppm, at least 90 ppm, at least 100

ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm, at least 225 ppm, at least 250

ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400

ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550

ppm, at least 575 ppm, at least 600 ppm, at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700

ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm, at least 850

ppm, at least 875 ppm, at least 900 ppm, at least 925 ppm, at least 950 ppm, at least 975 ppm, at least

1,000 ppm, at least 1,025 ppm, at least 1,050 ppm, at least 1075 ppm, at least 1,100 ppm, at least ,125

ppm, at least 1,150 ppm, at least 1,175 ppm, at least 1,200 ppm, at least 1,225 ppm, at least 1,250 ppm,

at least 1,275 ppm, at least 1,300 ppm, at least 1,325 ppm, at least 1,350 ppm, at least 1,375 ppm, at least

1,400 ppm, at least 1,425 ppm, at least 1,450 ppm, at least 1,475 ppm, at least 1,500 ppm, at least 1,600

ppm, at least 1,700 ppm, at least 1,800 ppm, at least 1,900 ppm, or at least 2,000 ppm. In yet other aspects

of this embodiment, a composition disclosed herein comprises hypochlorous acid in an amount of, e.g., at

most 0.01 ppm, at most 0.05 ppm, at most 0.10 ppm, at most 0.20 ppm, at most 0.30 ppm, at most 0.40

ppm, at most 0.50 ppm, at most 0.60 ppm, at most 0.70 ppm, at most 0.80 ppm, at most 0.90 ppm, at most

1 ppm, at most 10 ppm, at most 20 ppm, at most 30 ppm, at most 40 ppm, at most 50 ppm, at most 60

ppm, at most 70 ppm, at most 80 ppm, at most 90 ppm, at most 100 ppm, at most 125 ppm, at most 150

ppm, at most 175 ppm, at most 200 ppm, at most 225 ppm, at most 250 ppm, at most 275 ppm, at most

300 ppm, at most 325 ppm, at most 350 ppm, at most 375 ppm, at most 400 ppm, at most 425 ppm, at

most 450 ppm, at most 475 ppm, at most 500 ppm, at most 525 ppm, at most 550 ppm, at most 575 ppm,

at most 600 ppm, at most 625 ppm, at most 650 ppm, at most 675 ppm, at most 700 ppm, at most 725

ppm, at most 750 ppm, at most 775 ppm, at most 800 ppm, at most 825 ppm, at most 850 ppm, at most

875 ppm, at most 900 ppm, at most 925 ppm, at most 950 ppm, at most 975 ppm, at most 1,000 ppm, at

most 1,025 ppm, at most 1,050 ppm, at most 1075 ppm, at most 1,100 ppm, at most 1,125 ppm, at most

1,150 ppm, at most 1,175 ppm, at most 1,200 ppm, at most 1,225 ppm, at most 1,250 ppm, at most 1,275

ppm, at most 1,300 ppm, at most 1,325 ppm, at most 1,350 ppm, at most 1,375 ppm, at most 1,400 ppm, at most 1,425 ppm, at most 1,450 ppm, at most 1,475 ppm, at most 1,500 ppm, at most 1,600 ppm, at most

1,700 ppm, at most 1,800 ppm, at most 1,900 ppm, or at most 2,000 ppm.

[054] In still other aspects of this embodiment, a composition disclosed herein comprises hypochlorous

acid in an amount of from, e.g., about 0.01 ppm to about 0.1 ppm, about 0.01 ppm to about 0.2 ppm, about

0.01 ppm to about 0.25 ppm, about 0.01 ppm to about 0.3 ppm, about 0.01 ppm to about 0.4 ppm, about

0.01 ppm to about 0.5 ppm, about 0.01 ppm to about 1 ppm, about 0.01 ppm to about 5 ppm, about 0.01

ppm to about 10 ppm, about 0.01 ppm to about 15 ppm, about 0.01 ppm to about 20 ppm, about 0.01 ppm

to about 25 ppm, about 0.01 ppm to about 30 ppm, about 0.01 ppm to about 35 ppm, about 0.01 ppm to

about 40 ppm, about 0.01 ppm to about 45 ppm, about 0.01 ppm to about 50 ppm, about 0.05 ppm to about

0.1 ppm, about 0.05 ppm to about 0.5 ppm, about 0.05 ppm to about 1 ppm, about 0.05 ppm to about 5

ppm, about 0.05 ppm to about 10 ppm, about 0.05 ppm to about 15 ppm, about 0.05 ppm to about 20 ppm,

about 0.05 ppm to about 25 ppm, about 0,05 ppm to about 30 ppm, about 0.05 ppm to about 35 ppm, about

0.05 ppm to about 40 ppm, about 0.05 ppm to about 45 ppm, about 0.05 ppm to about 50 ppm, about 0.1

ppm to about 1 ppm, about 0.1 ppm to about 5 ppm, about 0.1 ppm to about 10 ppm, about 0.1 ppm to

about 15 ppm, about 0.1 ppm to about 20 ppm, about 0.1 ppm to about 25 ppm, about 0.1 ppm to about

30 ppm, about 0.1 ppm to about 35 ppm, about 0.1 ppm to about 40 ppm, about 0.1 ppm to about 45 ppm,

about 0.1 ppm to about 50 ppm, about 0.5 ppm to about 1 ppm, about 0.5 ppm to about 5 ppm, about 0.5

ppm to about 10 ppm, about 0.5 ppm to about 15 ppm, about 0.5 ppm to about 20 ppm, about 0.5 ppm to

about 25 ppm, about 0.5 ppm to about 30 ppm, about 0.5 ppm to about 35 ppm, about 0.5 ppm to about

40 ppm, about 0.5 ppm to about 45 ppm, about 0.5 ppm to about 50 ppm, about 0.5 ppm to about 55 ppm,

about 0.5 ppm to about 60 ppm, about 0.5 ppm to about 65 ppm, about 0.5 ppm to about 70 ppm, about

0.5 ppm to about 75 ppm, about 0.5 ppm to about 80 ppm, about 0.5 ppm to about 85 ppm, about 0.5 ppm

to about 90 ppm, about 0.5 ppm to about 95 ppm, about 0.5 ppm to about 100 ppm, about 0.75 ppm to

about 20 ppm, about 0.75 ppm to about 25 ppm, about 0.75 ppm to about 30 ppm, about 0.75 ppm to about

35 ppm, about 0.75 ppm to about 40 ppm, about 0.75 ppm to about 45 ppm, about 0.75 ppm to about 50

ppm, about 0.75 ppm to about 55 ppm, about 0.75 ppm to about 60 ppm, about 0.75 ppm to about 65 ppm,

about 0.75 ppm to about 70 ppm, about 0.75 ppm to about 75 ppm, about 0.75 ppm to about 80 ppm, about

0.75 ppm to about 85 ppm, about 0.75 ppm to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75

ppm to about 100 ppm, about 1 ppm to about 10 ppm, about 1 ppm to about 15 ppm, about 1 ppm to about

20 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 30 ppm, about 1 ppm to about 35 ppm, about

1 ppm to about 40 ppm, about 1 ppm to about 45 ppm, about 1 ppm to about 50 ppm, about 1 ppm to about

55 ppm, about 1 ppm to about 60 ppm, about 1 ppm to about 65 ppm, about 1 ppm to about 70 ppm, about

1 ppm to about 75 ppm, about 1 ppm to about 80 ppm, about 1 ppm to about 85 ppm, about 1 ppm to about

90 ppm, about 1 ppm to about 95 ppm, about 1 ppm to about 100 ppm, about 5 ppm to about 10 ppm,

about 5 ppm to about 15 ppm, about 5 ppm to about 20 ppm, about 5 ppm to about 25 ppm, about 5 ppm

to about 30 ppm, about 5 ppm to about 35 ppm, about 5 ppm to about 40 ppm, about 5 ppm to about 45

ppm, about 5 ppm to about 50 ppm, about 5 ppm to about 55 ppm, about 5 ppm to about 60 ppm, about 5

ppm to about 65 ppm, about 5 ppm to about 70 ppm, about 5 ppm to about 75 ppm, about 5 ppm to about

80 ppm, about 5 ppm to about 85 ppm, about 5 ppm to about 90 ppm, about 5 ppm to about 95 ppm, about

5 ppm to about 100 ppm, about 10 ppm to about 20 ppm, about 10 ppm to about 25 ppm, about 10 ppm to

WO wo 2020/252433 PCT/US2020/037665 18

about 30 ppm, about 10 ppm to about 35 ppm, about 10 ppm to about 40 ppm, about 10 ppm to about 45

ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 55 ppm, about 10 ppm to about 60 ppm, about

10 ppm to about 65 ppm, about 10 ppm to about 70 ppm, about 10 ppm to about 75 ppm, about 10 ppm to

about 80 ppm, about 10 ppm to about 85 ppm, about 10 ppm to about 90 ppm, about 10 ppm to about 95

ppm, or about 10 ppm to about 100 ppm.

[055] In other aspects of this embodiment, a composition disclosed herein comprises hypochlorous acid

in an amount of from, e.g., about 1 ppm to about 25 ppm, about 1 ppm to about 50 ppm, about 1 ppm to

about 75 ppm, about 1 ppm to about 100 ppm, about 1 ppm to about 125 ppm, about 1 ppm to about 150

ppm, about 1 ppm to about 175 ppm, about 1 ppm to about 200 ppm, about 1 ppm to about 225 ppm, about

1 ppm to about 250 ppm, about 1 ppm to about 275 ppm, about 1 ppm to about 300 ppm, about 1 ppm to

about 325 ppm, about 1 ppm to about 350 ppm, about 1 ppm to about 375 ppm, about 1 ppm to about 400

ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 75 ppm, about

10 ppm to about 100 ppm, about 10 ppm to about 125 ppm, about 10 ppm to about 150 ppm, about 10 ppm

to about 175 ppm, about 10 ppm to about 200 ppm, about 10 ppm to about 225 ppm, about 10 ppm to

about 250 ppm, about 10 ppm to about 275 ppm, about 10 ppm to about 300 ppm, about 10 ppm to about

325 ppm, about 10 ppm to about 350 ppm, about 10 ppm to about 375 ppm, about 10 ppm to about 400

ppm, about 25 ppm to about 50 ppm, about 25 ppm to about 75 ppm, about 25 ppm to about 100 ppm,

about 25 ppm to about 125 ppm, about 25 ppm to about 150 ppm, about 25 ppm to about 175 ppm, about

25 ppm to about 200 ppm, about 25 ppm to about 225 ppm, about 25 ppm to about 250 ppm, about 25 ppm

to about 275 ppm, about 25 ppm to about 300 ppm, about 25 ppm to about 325 ppm, about 25 ppm to

about 350 ppm, about 25 ppm to about 375 ppm, about 25 ppm to about 400 ppm, about 50 ppm to about

75 ppm, about 50 ppm to about 100 ppm, about 50 ppm to about 125 ppm, about 50 ppm to about 150

ppm, about 50 ppm to about 175 ppm, about 50 ppm to about 200 ppm, about 50 ppm to about 225 ppm,

about 50 ppm to about 250 ppm, about 50 ppm to about 275 ppm, about 50 ppm to about 300 ppm, about

50 ppm to about 325 ppm, about 50 ppm to about 350 ppm, about 50 ppm to about 375 ppm, about 50 ppm

to about 400 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125 ppm, about 75 ppm to

about 150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200 ppm, about 75 ppm to about

225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, about 75 ppm to about 300

ppm, about 75 ppm to about 325 ppm, about 75 ppm to about 350 ppm, about 75 ppm to about 375 ppm,

about 75 ppm to about 400 ppm, about 100 ppm to about 125 ppm, about 100 ppm to about 150 ppm,

about 100 ppm to about 175 ppm, about 100 ppm to about 200 ppm, about 100 ppm to about 225 ppm,

about 100 ppm to about 250 ppm, about 100 ppm to about 275 ppm, about 100 ppm to about 300 ppm,

about 100 ppm to about 325 ppm, about 100 ppm to about 350 ppm, about 100 ppm to about 375 ppm,

about 100 ppm to about 400 ppm, about 150 ppm to about 175 ppm, about 150 ppm to about 200 ppm,

about 150 ppm to about 225 ppm, about 150 ppm to about 250 ppm, about 150 ppm to about 275 ppm,

about 150 ppm to about 300 ppm, about 150 ppm to about 325 ppm, about 150 ppm to about 350 ppm,

about 150 ppm to about 375 ppm, about 150 ppm to about 400 ppm, about 200 ppm to about 225 ppm,

about 200 ppm to about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm,

about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm,

about 200 ppm to about 400 ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300 ppm,

WO wo 2020/252433 PCT/US2020/037665 19

about 250 ppm to about 325 ppm, about 250 ppm to about 350 ppm, about 250 ppm to about 375 ppm,

about 250 ppm to about 400 ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm,

about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 350 ppm to about 375 ppm,

about 350 ppm to about 400 ppm, or about 375 ppm to about 400 ppm.

[056] In other aspects of this embodiment, a composition disclosed herein comprises hypochlorous acid

in an amount of, e.g., about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, about 400 ppm

to about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about 400 ppm to

about 1,000 ppm, about 400 ppm to about 1,100 ppm, about 400 ppm to about 1,200 ppm, about 400 ppm

to about 1,300 ppm, about 400 ppm to about 1,400 ppm, about 400 ppm to about 1,500 ppm, about 400

ppm to about 1,600 ppm, about 400 ppm to about 1,700 ppm, about 400 ppm to about 1,800 ppm, about

400 ppm to about 1,900 ppm, about 400 ppm to about 2,000 ppm, about 500 ppm to about 600 ppm, about

500 ppm to about 700 ppm, about 500 ppm to about 800 ppm, about 500 ppm to about 900 ppm, about

500 ppm to about 1,000 ppm, about 500 ppm to about 1,100 ppm, about 500 ppm to about 1,200 ppm,

about 500 ppm to about 1,300 ppm, about 500 ppm to about 1,400 ppm, about 500 ppm to about 1,500

ppm, about 500 ppm to about 1,600 ppm, about 500 ppm to about 1,700 ppm, about 500 ppm to about

1,800 ppm, about 500 ppm to about 1,900 ppm, about 500 ppm to about 2,000 ppm, about 600 ppm to

about 700 ppm, about 600 ppm to about 800 ppm, about 600 ppm to about 900 ppm, about 600 ppm to

about 1,000 ppm, about 600 ppm to about 1,100 ppm, about 600 ppm to about 1,200 ppm, about 600 ppm

to about 1,300 ppm, about 600 ppm to about 1,400 ppm, about 600 ppm to about 1,500 ppm, about 600

ppm to about 1,600 ppm, about 600 ppm to about 1,700 ppm, about 600 ppm to about 1,800 ppm, about

600 ppm to about 1,900 ppm, about 600 ppm to about 2,000 ppm, about 700 ppm to about 800 ppm, about

700 ppm to about 900 ppm, about 700 ppm to about 1,000 ppm, about 700 ppm to about 1,100 ppm, about

700 ppm to about 1,200 ppm, about 700 ppm to about 1,300 ppm, about 700 ppm to about 1,400 ppm,

about 700 ppm to about 1,500 ppm, about 700 ppm to about 1,600 ppm, about 700 ppm to about 1,700

ppm, about 700 ppm to about 1,800 ppm, about 700 ppm to about 1,900 ppm, about 700 ppm to about

2,000 ppm, about 800 ppm to about 900 ppm, about 800 ppm to about 1,000 ppm, about 800 ppm to about

1,100 ppm, about 800 ppm to about 1,200 ppm, about 800 ppm to about 1,300 ppm, about 800 ppm to

about 1,400 ppm, about 800 ppm to about 1,500 ppm, about 800 ppm to about 1,600 ppm, about 800 ppm

to about 1,700 ppm, about 800 ppm to about 1,800 ppm, about 800 ppm to about 1,900 ppm, about 800

ppm to about 2,000 ppm, about 900 ppm to about 1,000 ppm, about 900 ppm to about 1,100 ppm, about

900 ppm to about 1,200 ppm, about 900 ppm to about 1,300 ppm, about 900 ppm to about 1,400 ppm,

about 900 ppm to about 1,500 ppm, about 900 ppm to about 1,600 ppm, about 900 ppm to about 1,700

ppm, about 900 ppm to about 1,800 ppm, about 900 ppm to about 1,900 ppm, about 900 ppm to about

2,000 ppm, about 1,000 ppm to about 1,100 ppm, about 1,000 ppm to about 1,200 ppm, about 1,000 ppm

to about 1,300 ppm, about 1,000 ppm to about 1,400 ppm, about 1,000 ppm to about 1,500 ppm, about

1,000 ppm to about 1,600 ppm, about 1,000 ppm to about 1,700 ppm, about 1,000 ppm to about 1,800

ppm, about 1,000 ppm to about 1,900 ppm, about 1,000 ppm to about 2,000 ppm, about 1,100 ppm to

about 1,200 ppm, about 1,100 ppm to about 1,300 ppm, about 1,100 ppm to about 1,400 ppm, about 1,100

ppm to about 1,500 ppm, about 1,100 ppm to about 1,600 ppm, about 1,100 ppm to about 1,700 ppm,

about 1,100 ppm to about 1,800 ppm, about 1,100 ppm to about 1,900 ppm, about 1,100 ppm to about

2,000 ppm, about 1,200 ppm to about 1,300 ppm, about 1,200 ppm to about 1,400 ppm, about 1,200 ppm

to about 1,500 ppm, about 1,200 ppm to about 1,600 ppm, about 1,200 ppm to about 1,700 ppm, about

1,200 ppm to about 1,800 ppm, about 1,200 ppm to about 1,900 ppm, about 1,200 ppm to about 2,000

ppm, about 1,300 ppm to about 1,400 ppm, about 1,300 ppm to about 1,500 ppm, about 1,300 ppm to

about 1,600 ppm, about 1,300 ppm to about 1,700 ppm, about 1,300 ppm to about 1,800 ppm, about 1,300

ppm to about 1,900 ppm, about 1,300 ppm to about 2,000 ppm, about 1,400 ppm to about 1,500 ppm,

about 1,400 ppm to about 1,600 ppm, about 1,400 ppm to about 1,700 ppm, about 1,400 ppm to about

1,800 ppm, about 1,400 ppm to about 1,900 ppm, about 1,400 ppm to about 2,000 ppm, about 1,500 ppm

to about 1,600 ppm, about 1,500 ppm to about 1,700 ppm, about 1,500 ppm to about 1,800 ppm, about

1,500 ppm to about 1,900 ppm, about 1,500 ppm to about 2,000 ppm, about 1,600 ppm to about 1,700

ppm, about 1,600 ppm to about 1,800 ppm, about 1,600 ppm to about 1,900 ppm, about 1,600 ppm to

about 2,000 ppm, about 1,700 ppm to about 1,800 ppm, about 1,700 ppm to about 1,900 ppm, about 1,700

ppm to about 2,000 ppm, about 1,800 ppm to about 1,900 ppm, about 1,800 ppm to about 2,000 ppm, or

about 1,900 ppm to about 2,000 ppm.

[057] In aspects of this embodiment, a composition disclosed herein comprises hypochlorous acid in an

amount of, e.g., about 0.001%, about 0.005%, about 0.01%, about 0.05%, about 0.10%, about 0.11%,

about 0.12%, about 0.13%, about 0.14%, about 0.15%, about 0.16%, about 0.17%, about 0.18%, about

0.19%, about 0.2%, about 0.25%, about 0.3%, about 0.35%, about 0.4%, about 0.45%, or about 0.5% by

weight of the composition. In other aspects of this embodiment, a composition disclosed herein comprises

hypochlorous acid in an amount of, e.g., at least 0.001%, at least 0.005%, at least 0.01%, at least 0.05%,

at least 0.10%, at least 0.11%, at least 0.12%, at least 0.13%, at least 0.14%, at least 0.15%, at least

0.16%, at least 0.17%, at least 0.18%, at least 0.19%, at least 0.2%, at least 0.25%, at least 0.3%, at least

0.35%, at least 0.4%, at least 0.45%, or at least 0.5% by weight of the composition. In yet other aspects of

this embodiment, a composition disclosed herein comprises hypochlorous acid in an amount of, e.g., at

most 0.001%, at most 0.005%, at most 0.01%, at most 0.05%, at most 0.10%, at most 0.11%, at most

0.12%, at most 0.13%, at most 0.14%, at most 0.15%, at most 0.16%, at most 0.17%, at most 0.18%, at

most 0.19%, at most 0.2%, at most 0.25%, at most 0.3%, at most 0.35%, at most 0.4%, at most 0.45%, or

at most 0.5% by weight of the composition. In still other aspects of this embodiment, a composition

disclosed herein comprises hypochlorous acid in an amount of, e.g., about 0.0010% to about 0.005%, about

0.0010% to about 0.010%, about 0.0010% to about 0.050%, about 0.0010% to about 0.10%, about 0.0010% to about 0.15%, about 0.0010% to about 0.20%, about 0.0010% to about 0.25%, about 0.0010%

to about 0.30%, about 0.0010% to about 0.35%, about 0.0010% to about 0.40%, about 0.0010% to about

0.45%, about 0.0010% to about 0.50%, about 0.010% to about 0.050%, about 0.010% to about 0.10%,

about 0.010% to about 0.15%, about 0.010% to about 0.20%, about 0.010% to about 0.25%, about 0.010%

to about 0.30%, about 0.010% to about 0.35%, about 0.010% to about 0.40%, about 0.010% to about

0.45%, about 0.010% to about 0.50%, about 0.10% to about 0.15%, about 0.10% to about 0.20%, about

0.10% to about 0.25%, about 0.10% to about 0.30%, about 0.10% to about 0.35%, about 0.10% to about

0.40%, about 0.10% to about 0.45%, about 0.10% to about 0.50%, about 0.20% to about 0.25%, about

0.20% to about 0.30%, about 0.20% to about 0.35%, about 0.20% to about 0.40%, about 0.20% to about

0.45%, about 0.20% to about 0.50%, about 0.30% to about 0.35%, about 0.30% to about 0.40%, about wo 2020/252433 WO PCT/US2020/037665 21

0.30% to about 0.45%, about 0.30% to about 0.50%, about 0.40% to about 0.45%, about 0.40% to about

0.50%, or about 0.40% to about 0.50% by weight of the composition.

[058] In one embodiment, a composition disclosed herein does not comprise hypochlorous acid.

[059] The concentration of hypochlorous acid in solution may be described as free available chlorine in

parts per million. Hypochlorous acid is in equilibrium with hypochlorite ions (OCI) and dissolved chlorine

gas (Cl2). The extent of the equilibrium is determined predominately by the salt concentration and pH of

the solution. Temperature also impacts the ratio of the free chlorine component. Therefore, both FAC and

pH need to be known to understand the amount of chlorine present as hypochlorous acid. In general, when

the pH range is about 4.0 to about 5.6, approximately 100% of the available chlorine is present as HOCI.

As the pH is lowered below about 4, there is an increase in dissolved chlorine gas (Cl2). Thus, at a pH of

about 3, about 90% of the available chlorine is present as hypochlorous acid, at a pH of about 2, about

75% of the available chlorine is present as hypochlorous acid, at a pH of about 1.5, about 50% of the

available chlorine is present as hypochlorous acid, while at a pH of about 1, about 25% of the available

chlorine is present as hypochlorous acid. As the pH is increase above about 5.6, there is an increase in

hypochlorite ions (OCF). Thus, at a pH a pH of about 6.5, about 90% of the available chlorine is present

as hypochlorous acid, at a pH of about 7, about 75% of the available chlorine is present as hypochlorous

acid, at a pH of about 7.5, about 50% of the available chlorine is present as hypochlorous acid, while at a

pH of about 8, about 25% of the available chlorine is present as hypochlorous acid.

[060] The chlorine amount may be measured by methods known in the art, such as a DPD colorimeter

method (Lamotte Company, Chestertown, Md.), a UV spectrophotometry method, or other known methods

established by the Environmental Protection Agency. In the DPD colorimeter method, a yellow color is

formed by the reaction of free chlorine With N,N-diethyl-p-phenylenediamine (DPD) and the intensity is

measured With a calibrated calorimeter that provides the output in parts per million. Further addition of

potassium iodide turns the solution a pink color to provide the total chlorine value.

[061] In an embodiment, a composition disclosed herein comprises an amount of free available chlorine

that provides a desired beneficial effect to a composition disclosed herein. For example, when a

composition comprising free available chlorine is formulated to be used in an undiluted state, the amount

of hypochlorous acid used is a microbiostatic effective amount. A microbiostatic effective amount of free

available chlorine is an amount that restrains, prohibits, or otherwise prevents microbial population growth

without eradicating the microbes. In an aspect of this embodiment, a microbiostatic effective amount of

free available chlorine is an amount that restrains, prohibits, or otherwise prevents microbial population

growth without eradicating the microbes to a degree that achieves disinfection or sterilization. Alternatively,

when a composition comprising free available chlorine is formulated to be used in methods where the

composition will be diluted, the amount of free available chlorine used in such concentrated formulations

can be any amount that does not cause precipitation of components in the composition disclosed herein or

otherwise create unwanted effects that make the composition inoperable.

[062] In aspects of this embodiment, a composition disclosed herein comprises free available chlorine in

an amount of, e.g., 0.01 ppm, 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm,

0.40 ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80 ppm, 0.85 ppm,

0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm,

50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm, 95 ppm, 100 ppm, 125 ppm,

ppm, or 2,000 ppm. In other aspects of this embodiment, a composition disclosed herein comprises free

available chlorine in an amount of, e.g., at least 0.01 ppm, at least 0.05 ppm, at least 0.10 ppm, at least

1,000 ppm, at least 1,025 ppm, at least 1,050 ppm, at least 1075 ppm, at least 1,100 ppm, at least 1,125

of this embodiment, a composition disclosed herein comprises free available chlorine in an amount of, e.g.,

at most 0.01 ppm, at most 0.05 ppm, at most 0.10 ppm, at most 0.20 ppm, at most 0.30 ppm, at most 0.40

ppm, at most 1,300 ppm, at most 1,325 ppm, at most 1,350 ppm, at most 1,375 ppm, at most 1,400 ppm,

PCT/US2020/037665 23

at most 1,425 ppm, at most 1,450 ppm, at most 1,475 ppm, at most 1,500 ppm, at most 1,600 ppm, at most

1,700 ppm, at most 1,800 ppm, at most 1,900 ppm, or at most 2,000 ppm.

[063] In still other aspects of this embodiment, a composition disclosed herein comprises free available

chlorine in an amount of from, e.g., about 0.01 ppm to about 0.1 ppm, about 0.01 ppm to about 0.2 ppm,

about 0.01 ppm to about 0.25 ppm, about 0.01 ppm to about 0.3 ppm, about 0.01 ppm to about 0.4 ppm,

about 0.01 ppm to about 0.5 ppm, about 0.01 ppm to about 0.1 ppm, about 0.01 ppm to about 0.5 ppm,

about 0.01 ppm to about 1 ppm, about 0.01 ppm to about 5 ppm, about 0.01 ppm to about 10 ppm, about

0.01 ppm to about 15 ppm, about 0.01 ppm to about 20 ppm, about 0.01 ppm to about 25 ppm, about 0.01

ppm to about 30 ppm, about 0.01 ppm to about 35 ppm, about 0.01 ppm to about 40 ppm, about 0.01 ppm

to about 45 ppm, about 0.01 ppm to about 50 ppm, about 0.05 ppm to about 0.1 ppm, about 0.05 ppm to

about 0.5 ppm, about 0.05 ppm to about 1 ppm, about 0.05 ppm to about 5 ppm, about 0.05 ppm to about

10 ppm, about 0.05 ppm to about 15 ppm, about 0.05 ppm to about 20 ppm, about 0.05 ppm to about 25

ppm, about 0.05 ppm to about 30 ppm, about 0.05 ppm to about 35 ppm, about 0.05 ppm to about 40 ppm,

about 0.05 ppm to about 45 ppm, about 0.05 ppm to about 50 ppm, about 0.1 ppm to about 1 ppm, about

0.1 ppm to about 5 ppm, about 0.1 ppm to about 10 ppm, about 0.1 ppm to about 15 ppm, about 0.1 ppm

to about 20 ppm, about 0.1 ppm to about 25 ppm, about 0.1 ppm to about 30 ppm, about 0.1 ppm to about

35 ppm, about 0.1 ppm to about 40 ppm, about 0.1 ppm to about 45 ppm, about 0.1 ppm to about 50 ppm,

about 0.5 ppm to about 1 ppm, about 0.5 ppm to about 5 ppm, about 0.5 ppm to about 10 ppm, about 0.5

ppm to about 15 ppm, about 0.5 ppm to about 20 ppm, about 0.5 ppm to about 25 ppm, about 0.5 ppm to

about 30 ppm, about 0.5 ppm to about 35 ppm, about 0.5 ppm to about 40 ppm, about 0.5 ppm to about

45 ppm, about 0.5 ppm to about 50 ppm, about 0.5 ppm to about 55 ppm, about 0.5 ppm to about 60 ppm,

about 0.5 ppm to about 65 ppm, about 0.5 ppm to about 70 ppm, about 0.5 ppm to about 75 ppm, about

0.5 ppm to about 80 ppm, about 0.5 ppm to about 85 ppm, about 0.5 ppm to about 90 ppm, about 0.5 ppm

to about 95 ppm, about 0.5 ppm to about 100 ppm, about 0.75 ppm to about 20 ppm, about 0.75 ppm to

about 25 ppm, about 0.75 ppm to about 30 ppm, about 0.75 ppm to about 35 ppm, about 0.75 ppm to about

40 ppm, about 0.75 ppm to about 45 ppm, about 0.75 ppm to about 50 ppm, about 0.75 ppm to about 55

ppm, about 0.75 ppm to about 60 ppm, about 0.75 ppm to about 65 ppm, about 0.75 ppm to about 70 ppm,

about 0.75 ppm to about 75 ppm, about 0.75 ppm to about 80 ppm, about 0.75 ppm to about 85 ppm, about

0.75 ppm to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75 ppm to about 100 ppm, about 1

ppm to about 10 ppm, about 1 ppm to about 15 ppm, about 1 ppm to about 20 ppm, about 1 ppm to about

25 ppm, about 1 ppm to about 30 ppm, about 1 ppm to about 35 ppm, about 1 ppm to about 40 ppm, about

1 ppm to about 45 ppm, about 1 ppm to about 50 ppm, about 1 ppm to about 55 ppm, about 1 ppm to about

60 ppm, about 1 ppm to about 65 ppm, about 1 ppm to about 70 ppm, about 1 ppm to about 75 ppm, about

1 ppm to about 80 ppm, about 1 ppm to about 85 ppm, about 1 ppm to about 90 ppm, about 1 ppm to about

95 ppm, about 1 ppm to about 100 ppm, about 5 ppm to about 10 ppm, about 5 ppm to about 15 ppm,

about 5 ppm to about 20 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 30 ppm, about 5 ppm

to about 35 ppm, about 5 ppm to about 40 ppm, about 5 ppm to about 45 ppm, about 5 ppm to about 50

ppm, about 5 ppm to about 55 ppm, about 5 ppm to about 60 ppm, about 5 ppm to about 65 ppm, about 5

ppm to about 70 ppm, about 5 ppm to about 75 ppm, about 5 ppm to about 80 ppm, about 5 ppm to about

85 ppm, about 5 ppm to about 90 ppm, about 5 ppm to about 95 ppm, about 5 ppm to about 100 ppm, wo 2020/252433 WO PCT/US2020/037665 24 about 10 ppm to about 20 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 30 ppm, about 10 ppm to about 35 ppm, about 10 ppm to about 40 ppm, about 10 ppm to about 45 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 55 ppm, about 10 ppm to about 60 ppm, about 10 ppm to about 65 ppm, about 10 ppm to about 70 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 80 ppm, about

10 ppm to about 85 ppm, about 10 ppm to about 90 ppm, about 10 ppm to about 95 ppm, or about 10 ppm

to about 100 ppm.

[064] In other aspects of this embodiment, a composition disclosed herein comprises free available

chlorine in an amount of from, e.g., about 1 ppm to about 25 ppm, about 1 ppm to about 50 ppm, about 1

ppm to about 75 ppm, about 1 ppm to about 100 ppm, about 1 ppm to about 125 ppm, about 1 ppm to

about 150 ppm, about 1 ppm to about 175 ppm, about 1 ppm to about 200 ppm, about 1 ppm to about 225

ppm, about 1 ppm to about 250 ppm, about 1 ppm to about 275 ppm, about 1 ppm to about 300 ppm, about

1 ppm to about 325 ppm, about 1 ppm to about 350 ppm, about 1 ppm to about 375 ppm, about 1 ppm to

about 400 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 75

ppm, about 10 ppm to about 100 ppm, about 10 ppm to about 125 ppm, about 10 ppm to about 150 ppm,

about 10 ppm to about 175 ppm, about 10 ppm to about 200 ppm, about 10 ppm to about 225 ppm, about

10 ppm to about 250 ppm, about 10 ppm to about 275 ppm, about 10 ppm to about 300 ppm, about 10 ppm

to about 325 ppm, about 10 ppm to about 350 ppm, about 10 ppm to about 375 ppm, about 10 ppm to

about 400 ppm, about 25 ppm to about 50 ppm, about 25 ppm to about 75 ppm, about 25 ppm to about

100 ppm, about 25 ppm to about 125 ppm, about 25 ppm to about 150 ppm, about 25 ppm to about 175

ppm, about 25 ppm to about 200 ppm, about 25 ppm to about 225 ppm, about 25 ppm to about 250 ppm,

about 25 ppm to about 275 ppm, about 25 ppm to about 300 ppm, about 25 ppm to about 325 ppm, about

25 ppm to about 350 ppm, about 25 ppm to about 375 ppm, about 25 ppm to about 400 ppm, about 50 ppm

to about 75 ppm, about 50 ppm to about 100 ppm, about 50 ppm to about 125 ppm, about 50 ppm to about

150 ppm, about 50 ppm to about 175 ppm, about 50 ppm to about 200 ppm, about 50 ppm to about 225

ppm, about 50 ppm to about 250 ppm, about 50 ppm to about 275 ppm, about 50 ppm to about 300 ppm,

about 50 ppm to about 325 ppm, about 50 ppm to about 350 ppm, about 50 ppm to about 375 ppm, about

50 ppm to about 400 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125 ppm, about 75 ppm

to about 150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200 ppm, about 75 ppm to

about 225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, about 75 ppm to about

300 ppm, about 75 ppm to about 325 ppm, about 75 ppm to about 350 ppm, about 75 ppm to about 375

ppm, about 75 ppm to about 400 ppm, about 100 ppm to about 125 ppm, about 100 ppm to about 150 ppm,

about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400 ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300 ppm, about 250 ppm to about 325 ppm, about 250 ppm to about 350 ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400 ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, or about 375 ppm to about 400 ppm.

[065] In other aspects of this embodiment, a composition disclosed herein comprises free available

chlorine in an amount of, e.g., about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, about

400 ppm to about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about

400 ppm to about 1,000 ppm, about 400 ppm to about 1,100 ppm, about 400 ppm to about 1,200 ppm,

about 400 ppm to about 1,300 ppm, about 400 ppm to about 1,400 ppm, about 400 ppm to about 1,500

ppm, about 400 ppm to about 1,600 ppm, about 400 ppm to about 1,700 ppm, about 400 ppm to about

1,800 ppm, about 400 ppm to about 1,900 ppm, about 400 ppm to about 2,000 ppm, about 500 ppm to

about 600 ppm, about 500 ppm to about 700 ppm, about 500 ppm to about 800 ppm, about 500 ppm to

about 900 ppm, about 500 ppm to about 1,000 ppm, about 500 ppm to about 1,100 ppm, about 500 ppm

to about 1,200 ppm, about 500 ppm to about 1,300 ppm, about 500 ppm to about 1,400 ppm, about 500

ppm to about 1,500 ppm, about 500 ppm to about 1,600 ppm, about 500 ppm to about 1,700 ppm, about

500 ppm to about 1,800 ppm, about 500 ppm to about 1,900 ppm, about 500 ppm to about 2,000 ppm,

about 600 ppm to about 700 ppm, about 600 ppm to about 800 ppm, about 600 ppm to about 900 ppm,

about 600 ppm to about 1,000 ppm, about 600 ppm to about 1,100 ppm, about 600 ppm to about 1,200

ppm, about 600 ppm to about 1,300 ppm, about 600 ppm to about 1,400 ppm, about 600 ppm to about

1,500 ppm, about 600 ppm to about 1,600 ppm, about 600 ppm to about 1,700 ppm, about 600 ppm to

about 1,800 ppm, about 600 ppm to about 1,900 ppm, about 600 ppm to about 2,000 ppm, about 700 ppm

to about 800 ppm, about 700 ppm to about 900 ppm, about 700 ppm to about 1,000 ppm, about 700 ppm

to about 1,100 ppm, about 700 ppm to about 1,200 ppm, about 700 ppm to about 1,300 ppm, about 700

ppm to about 1,400 ppm, about 700 ppm to about 1,500 ppm, about 700 ppm to about 1,600 ppm, about

700 ppm to about 1,700 ppm, about 700 ppm to about 1,800 ppm, about 700 ppm to about 1,900 ppm,

about 700 ppm to about 2,000 ppm, about 800 ppm to about 900 ppm, about 800 ppm to about 1,000 ppm,

about 800 ppm to about 1,100 ppm, about 800 ppm to about 1,200 ppm, about 800 ppm to about 1,300

ppm, about 800 ppm to about 1,400 ppm, about 800 ppm to about 1,500 ppm, about 800 ppm to about

1,600 ppm, about 800 ppm to about 1,700 ppm, about 800 ppm to about 1,800 ppm, about 800 ppm to

about 1,900 ppm, about 800 ppm to about 2,000 ppm, about 900 ppm to about 1,000 ppm, about 900 ppm

to about 1,100 ppm, about 900 ppm to about 1,200 ppm, about 900 ppm to about 1,300 ppm, about 900

ppm to about 1,400 ppm, about 900 ppm to about 1,500 ppm, about 900 ppm to about 1,600 ppm, about

900 ppm to about 1,700 ppm, about 900 ppm to about 1,800 ppm, about 900 ppm to about 1,900 ppm,

about 900 ppm to about 2,000 ppm, about 1,000 ppm to about 1,100 ppm, about 1,000 ppm to about 1,200

ppm, about 1,000 ppm to about 1,300 ppm, about 1,000 ppm to about 1,400 ppm, about 1,000 ppm to

about 1,500 ppm, about 1,000 ppm to about 1,600 ppm, about 1,000 ppm to about 1,700 ppm, about 1,000

ppm to about 1,800 ppm, about 1,000 ppm to about 1,900 ppm, about 1,000 ppm to about 2,000 ppm,

about 1,100 ppm to about 1,200 ppm, about 1,100 ppm to about 1,300 ppm, about 1,100 ppm to about

1,400 ppm, about 1,100 ppm to about 1,500 ppm, about 1,100 ppm to about 1,600 ppm, about 1,100 ppm

WO wo 2020/252433 PCT/US2020/037665 26

to about 1,700 ppm, about 1,100 ppm to about 1,800 ppm, about 1,100 ppm to about 1,900 ppm, about

1,100 ppm to about 2,000 ppm, about 1,200 ppm to about 1,300 ppm, about 1,200 ppm to about 1,400

ppm, about 1,200 ppm to about 1,500 ppm, about 1,200 ppm to about 1,600 ppm, about 1,200 ppm to

about 1,700 ppm, about 1,200 ppm to about 1,800 ppm, about 1,200 ppm to about 1,900 ppm, about 1,200

ppm to about 2,000 ppm, about 1,300 ppm to about 1,400 ppm, about 1,300 ppm to about 1,500 ppm,

about 1,300 ppm to about 1,600 ppm, about 1,300 ppm to about 1,700 ppm, about 1,300 ppm to about

1,800 ppm, about 1,300 ppm to about 1,900 ppm, about 1,300 ppm to about 2,000 ppm, about 1,400 ppm

to about 1,500 ppm, about 1,400 ppm to about 1,600 ppm, about 1,400 ppm to about 1,700 ppm, about

1,400 ppm to about 1,800 ppm, about 1,400 ppm to about 1,900 ppm, about 1,400 ppm to about 2,000

ppm, about 1,500 ppm to about 1,600 ppm, about 1,500 ppm to about 1,700 ppm, about 1,500 ppm to

about 1,800 ppm, about 1,500 ppm to about 1,900 ppm, about 1,500 ppm to about 2,000 ppm, about 1,600

ppm to about 1,700 ppm, about 1,600 ppm to about 1,800 ppm, about 1,600 ppm to about 1,900 ppm,

about 1,600 ppm to about 2,000 ppm, about 1,700 ppm to about 1,800 ppm, about 1,700 ppm to about

1,900 ppm, about 1,700 ppm to about 2,000 ppm, about 1,800 ppm to about 1,900 ppm, about 1,800 ppm

to about 2,000 ppm, or about 1,900 ppm to about 2,000 ppm.

[066] In aspects of this embodiment, a composition disclosed herein comprises free available chlorine in

an amount of, e.g., about 0.001%, about 0.005%, about 0.01%, about 0.05%, about 0.001%, about 0.01%,

about 0.10%, about 0.11%, about 0.12%, about 0.13%, about 0.14%, about 0.15%, about 0.16%, about

0.17%, about 0.18%, about 0.19%, about 0.2%, about 0.25%, about 0.3%, about 0.35%, about 0.4%, about

0.45%, or about 0.5% by weight of the composition. In other aspects of this embodiment, a composition

disclosed herein comprises free available chlorine in an amount of, e.g., at least 0.001%, at least 0.005%,

at least 0.01%, at least 0.05%, at least 0.10%, at least 0.11%, at least 0.12%, at least 0.13%, at least

0.14%, at least 0.15%, at least 0.16%, at least 0.17%, at least 0.18%, at least 0.19%, at least 0.2%, at least

0.25%, at least 0.3%, at least 0.35%, at least 0.4%, at least 0.45%, or at least 0.5% by weight of the

composition. In yet other aspects of this embodiment, a composition disclosed herein comprises free

available chlorine in an amount of, e.g., at most 0.001%, at most 0.005%, at most 0.01%, at most 0.05%,

at most 0.10%, at most 0.11%, at most 0.12%, at most 0.13%, at most 0.14%, at most 0.15%, at most

0.16%, at most 0.17%, at most 0.18%, at most 0.19%, at most 0.2%, at most 0.25%, at most 0.3%, at most

0.35%, at most 0.4%, at most 0.45%, or at most 0.5% by weight of the composition. In still other aspects

about 0.0010% to about 0.005%, about 0.0010% to about 0.010%, about 0.0010% to about 0.050%, about

0.0010% to about 0.10%, about 0.0010% to about 0.15%, about 0.0010% to about 0.20%, about 0.0010%

to about 0.25%, about 0.0010% to about 0.30%, about 0.0010% to about 0.35%, about 0.0010% to about

0.40%, about 0.0010% to about 0.45%, about 0.0010% to about 0.50%, about 0.010% to about 0.050%,

about 0.010% to about 0.10%, about 0.010% to about 0.15%, about 0.010% to about 0.20%, about 0.010%

to about 0.25%, about 0.010% to about 0.30%, about 0.010% to about 0.35%, about 0.010% to about

0.40%, about 0.010% to about 0.45%, about 0.010% to about 0.50%, about 0.10% to about 0.15%, about

0.10% to about 0.20%, about 0.10% to about 0.25%, about 0.10% to about 0.30%, about 0.10% to about

0.35%, about 0.10% to about 0.40%, about 0.10% to about 0.45%, about 0.10% to about 0.50%, about

0.20% to about 0.25%, about 0.20% to about 0.30%, about 0.20% to about 0.35%, about 0.20% to about

PCT/US2020/037665 27

0.40%, about 0.20% to about 0.45%, about 0.20% to about 0.50%, about 0.30% to about 0.35%, about

0.30% to about 0.40%, about 0.30% to about 0.45%, about 0.30% to about 0.50%, about 0.40% to about

0.45%, about 0.40% to about 0.50%, or about 0.40% to about 0.50% by weight of the composition.

[067] In one embodiment, a composition disclosed herein does not comprise free available chlorine.

[068] In one embodiment, a composition disclosed herein does not comprise ozone. In another embodiment, a composition disclosed herein can comprise ozone.

[069] In one embodiment, a composition disclosed herein does not comprise hydrogen peroxide. In

another embodiment, a composition disclosed herein can comprise hydrogen peroxide.

[070] A composition disclosed herein may comprise a quaternary compound. Non-limiting examples of

a quaternary compound includes a dialkyl quaternary compound and a polyether fatty quaternary

compound. Examples of a dialkyl quaternary compound includes, didodecyldimethylammonium chloride

and di-n-alkyldimethyl ammonium chloride. A quaternary compound includes a silicon quaternary

compound. Non-limiting examples of a silicon quaternary compound includes a silicone dialkyl quaternary

compound and a silicone polyether fatty quaternary compound. Examples of a silicone dialkyl quaternary

compound includes, without limitation, dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride

(DTSACI) and tetradecyldimethyl-(3-trimethoxysilyl)propy ammonium chloride (TTSACI). Examples of

other quaternary compounds include, without limitation, a quaternary ammonium salt, such as, e.g.,

Quaternium-15 and Quaternium-18 and a polycationic polymer, such as, e.g., cetylpyridinium salt, like

cetylpyridinium chloride or cetylpyridinium bromide, and a polyquaternium like Polyquaterniums 1, 2, 3, 4,

o5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, and 47. Other none-limiting examples of a quaternary

compound include those with the formula NR4+, where R is an alkyl or an aryl group.

[071] In one embodiment, a single quaternary compound or silicon quaternary compound is present in a

composition disclosed herein. In another embodiment, a plurality of quaternary compounds or silicon

quaternary compounds is present in a composition disclosed herein. In aspects of this embodiment, a

composition disclosed herein comprises, e.g., one or more quaternary compounds or silicon quaternary

compounds, two or more quaternary compounds or silicon quaternary compounds, three or more quaternary compounds or silicon quaternary compounds, four or more quaternary compounds or silicon

quaternary compounds or five or more quaternary compounds or silicon quaternary compounds. In other

aspects of this embodiment, a composition disclosed herein comprises, e.g., only one quaternary

compound or silicon quaternary compound, at most two quaternary compounds or silicon quaternary

compounds, at most three quaternary compounds or silicon quaternary compounds, at most four quaternary compounds or silicon quaternary compounds, or at most five quaternary compounds or silicon

quaternary compounds. In yet other aspects of this embodiment, a composition disclosed herein comprises

from, e.g., 1 to 2 quaternary compounds or silicon quaternary compounds, 1 to 3 quaternary compounds

or silicon quaternary compounds, 1 to 4 quaternary compounds or silicon quaternary compounds, 1 to 5 quaternary compounds or silicon quaternary compounds, 2 to 3 quaternary compounds or silicon quaternary compounds, 2 to 4 quaternary compounds or silicon quaternary compounds, 2 to 5 quaternary compounds or silicon quaternary compounds, 3 to 4 quaternary compounds or silicon quaternary compounds, 3 to 5 quaternary compounds or silicon quaternary compounds or 4 to 5 quaternary compounds or silicon quaternary compounds.

[072] In an embodiment, a composition disclosed herein comprises an amount of a quaternary compound

or silicon quaternary compound that provides a desired beneficial effect to a composition disclosed herein.

For example, when a composition comprising a quaternary compound or silicon quaternary compound is

formulated to be used in an undiluted state, the amount of a quaternary compound or silicon quaternary

compound used is a microbiostatic effective amount. A microbiostatic effective amount of a quaternary

compound or silicon quaternary compound is an amount that restrains, prohibits, or otherwise prevents

microbial population growth without eradicating the microbes. In an aspect of this embodiment, a

microbiostatic effective amount of a quaternary compound or silicon quaternary compound is an amount

that restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbes

to a degree that achieves disinfection or sterilization. Alternatively, when a composition comprising a

quaternary compound or silicon quaternary compound is formulated to be used in methods where the

composition will be diluted, the amount of a quaternary compound or silicon quaternary compound used in

such concentrated formulations can be any amount that does not cause precipitation of components in the

composition disclosed herein or otherwise create unwanted effects that make the composition inoperable.

[073] In aspects of this embodiment, a composition disclosed herein comprises a quaternary compound

or silicon quaternary compound in an amount of, e.g., 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm,

0.30 ppm, 0.35 ppm, 0.40 ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75 ppm,

0.80 ppm, 0.85 ppm, 0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35

ppm, 40 ppm, 45 ppm, 50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm, 95

ppm, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm,

350 ppm, 375 ppm, 400 ppm, 425 ppm, 450 ppm, 475 ppm, 500 ppm, 525 ppm, 550 ppm, 575 ppm, 600

ppm, 625 ppm, 650 ppm, 675 ppm, 700 ppm, 725 ppm, 750 ppm, 775 ppm, 800 ppm, 825 ppm, 850 ppm,

875 ppm, 900 ppm, 925 ppm, 950 ppm, 975 ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm, 1075 ppm, 1,100

ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm, 1,200 ppm, 1,225 ppm, 1,250 ppm, 1,275 ppm, 1,300 ppm, 1,325

ppm, 1,350 ppm, 1,375 ppm, 1,400 ppm, 1,425 ppm, 1,450 ppm, 1,475 ppm, 1,500 ppm, 1,600 ppm, 1,700

ppm, 1,800 ppm, 1,900 ppm, or 2,000 ppm. In other aspects of this embodiment, a composition disclosed

herein comprises a quaternary compound or silicon quaternary compound in an amount of, e.g., at least

0.05 ppm, at least 0.10 ppm, at least 0.20 ppm, at least 0.30 ppm, at least 0.40 ppm, at least 0.50 ppm, at

least 0.60 ppm, at least 0.70 ppm, at least 0.80 ppm, at least 0.90 ppm, at least 1 ppm, at least 10 ppm, at

least 20 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, at least 60 ppm, at least 70 ppm, at least

80 ppm, at least 90 ppm, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least

200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least

350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least

500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least 625 ppm, at least

650 ppm, atleast 675ppm,atleast 700ppm,atleast 725 ppm,atleast 750 ppm,at least 775 ppm, at least

800 ppm, at least 825 ppm, at least 850 ppm, at least 875 ppm, at least 900 ppm, at least 925 ppm, at least

950 ppm, at least 975 ppm, at least 1,000 ppm, at least 1,025 ppm, at least 1,050 ppm, at least 1075 ppm,

at least 1,100 ppm, at least 1,125 ppm, at least 1,150 ppm, at least 1,175 ppm, at least 1,200 ppm, at least

1,225 ppm, at least 1,250 ppm, at least 1,275 ppm, at least 1,300 ppm, at least 1,325 ppm, at least 1,350

ppm, at least 1,375 ppm, at least 1,400 ppm, at least 1,425 ppm, at least 1,450 ppm, at least 1,475 ppm,

at least 1,500 ppm, at least 1,600 ppm, at least 1,700 ppm, at least 1,800 ppm, at least 1,900 ppm, or at

least 2,000 ppm. In yet other aspects of this embodiment, a composition disclosed herein comprises a

quaternary compound or silicon quaternary compound in an amount of, e.g., at most 0.05 ppm, at most

0.10 ppm, at most 0.20 ppm, at most 0.30 ppm, at most 0.40 ppm, at most 0.50 ppm, at most 0.60 ppm, at

most 0.70 ppm, at most 0.80 ppm, at most 0.90 ppm, at most 1 ppm, at most 10 ppm, at most 20 ppm, at

most 30 ppm, at most 40 ppm, at most 50 ppm, at most 60 ppm, at most 70 ppm, at most 80 ppm, at most

90 ppm, at most 100 ppm, at most 125 ppm, at most 150 ppm, at most 175 ppm, at most 200 ppm, at most

225 ppm, at most 250 ppm, at most 275 ppm, at most 300 ppm, at most 325 ppm, at most 350 ppm, at

most 375 ppm, at most 400 ppm, at most 425 ppm, at most 450 ppm, at most 475 ppm, at most 500 ppm,

at most 525 ppm, at most 550 ppm, at most 575 ppm, at most 600 ppm, at most 625 ppm, at most 650

ppm, at most 675 ppm, at most 700 ppm, at most 725 ppm, at most 750 ppm, at most 775 ppm, at most

800 ppm, at most 825 ppm, at most 850 ppm, at most 875 ppm, at most 900 ppm, at most 925 ppm, at

most 950 ppm, at most 975 ppm, at most 1,000 ppm, at most 1,025 ppm, at most 1,050 ppm, at most 1075

ppm, at most 1,100 ppm, at most 1,125 ppm, at most 1,150 ppm, at most 1,175 ppm, at most 1,200 ppm,

at most 1,225 ppm, at most 1,250 ppm, at most 1,275 ppm, at most 1,300 ppm, at most 1,325 ppm, at most

1,350 ppm, at most 1,375 ppm, at most 1,400 ppm, at most 1,425 ppm, at most 1,450 ppm, at most 1,475

ppm, at most 1,500 ppm, at most 1,600 ppm, at most 1,700 ppm, at most 1,800 ppm, at most 1,900 ppm,

or at most 2,000 ppm.

[074] In still other aspects of this embodiment, a composition disclosed herein comprises a quaternary

compound or silicon quaternary compound in an amount of from, e.g., about 0.5 ppm to about 20 ppm,

about 0.5 ppm to about 25 ppm, about 0.5 ppm to about 30 ppm, about 0.5 ppm to about 35 ppm, about

0.5 ppm to about 40 ppm, about 0.5 ppm to about 45 ppm, about 0.5 ppm to about 50 ppm, about 0.5 ppm

to about 55 ppm, about 0.5 ppm to about 60 ppm, about 0.5 ppm to about 65 ppm, about 0.5 ppm to about

70 ppm, about 0.5 ppm to about 75 ppm, about 0.5 ppm to about 80 ppm, about 0.5 ppm to about 85 ppm,

about 0.5 ppm to about 90 ppm, about 0.5 ppm to about 95 ppm, about 0.5 ppm to about 100 ppm, about

0.75 ppm to about 20 ppm, about 0.75 ppm to about 25 ppm, about 0.75 ppm to about 30 ppm, about 0.75

ppm to about 35 ppm, about 0.75 ppm to about 40 ppm, about 0.75 ppm to about 45 ppm, about 0.75 ppm

to about 50 ppm, about 0.75 ppm to about 55 ppm, about 0.75 ppm to about 60 ppm, about 0.75 ppm to

about 65 ppm, about 0.75 ppm to about 70 ppm, about 0.75 ppm to about 75 ppm, about 0.75 ppm to about

80 ppm, about 0.75 ppm to about 85 ppm, about 0.75 ppm to about 90 ppm, about 0.75 ppm to about 95

ppm, about 0.75 ppm to about 100 ppm, about 1 ppm to about 20 ppm, about 1 ppm to about 25 ppm,

about 1 ppm to about 30 ppm, about 1 ppm to about 35 ppm, about 1 ppm to about 40 ppm, about 1 ppm

to about 45 ppm, about 1 ppm to about 50 ppm, about 1 ppm to about 55 ppm, about 1 ppm to about 60

ppm, about 1 ppm to about 65 ppm, about 1 ppm to about 70 ppm, about 1 ppm to about 75 ppm, about 1

WO wo 2020/252433 PCT/US2020/037665 30

ppm to about 80 ppm, about 1 ppm to about 85 ppm, about 1 ppm to about 90 ppm, about 1 ppm to about

95 ppm, about 1 ppm to about 100 ppm, about 5 ppm to about 20 ppm, about 5 ppm to about 25 ppm,

about 5 ppm to about 30 ppm, about 5 ppm to about 35 ppm, about 5 ppm to about 40 ppm, about 5 ppm

to about 45 ppm, about 5 ppm to about 50 ppm, about 5 ppm to about 55 ppm, about 5 ppm to about 60

ppm, about 5 ppm to about 65 ppm, about 5 ppm to about 70 ppm, about 5 ppm to about 75 ppm, about 5

ppm to about 80 ppm, about 5 ppm to about 85 ppm, about 5 ppm to about 90 ppm, about 5 ppm to about

95 ppm, about 5 ppm to about 100 ppm, about 10 ppm to about 20 ppm, about 10 ppm to about 25 ppm,

about 10 ppm to about 30 ppm, about 10 ppm to about 35 ppm, about 10 ppm to about 40 ppm, about 10

ppm to about 45 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 55 ppm, about 10 ppm to

about 60 ppm, about 10 ppm to about 65 ppm, about 10 ppm to about 70 ppm, about 10 ppm to about 75

ppm, about 10 ppm to about 80 ppm, about 10 ppm to about 85 ppm, about 10 ppm to about 90 ppm, about

10 ppm to about 95 ppm, or about 10 ppm to about 100 ppm.

[075] In other aspects of this embodiment, a composition disclosed herein comprises a quaternary

compound or silicon quaternary compound in an amount of from, e.g., about 1 ppm to about 25 ppm, about

1 ppm to about 50 ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 100 ppm, about 1 ppm to

about 125 ppm, about 1 ppm to about 150 ppm, about 1 ppm to about 175 ppm, about 1 ppm to about 200

ppm, about 1 ppm to about 225 ppm, about 1 ppm to about 250 ppm, about 1 ppm to about 275 ppm, about

1 ppm to about 300 ppm, about 1 ppm to about 325 ppm, about 1 ppm to about 350 ppm, about 1 ppm to

about 375 ppm, about 1 ppm to about 400 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50

ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 100 ppm, about 10 ppm to about 125 ppm,

about 10 ppm to about 150 ppm, about 10 ppm to about 175 ppm, about 10 ppm to about 200 ppm, about

10 ppm to about 225 ppm, about 10 ppm to about 250 ppm, about 10 ppm to about 275 ppm, about 10 ppm

to about 300 ppm, about 10 ppm to about 325 ppm, about 10 ppm to about 350 ppm, about 10 ppm to

about 375 ppm, about 10 ppm to about 400 ppm, about 25 ppm to about 50 ppm, about 25 ppm to about

75 ppm, about 25 ppm to about 100 ppm, about 25 ppm to about 125 ppm, about 25 ppm to about 150

ppm, about 25 ppm to about 175 ppm, about 25 ppm to about 200 ppm, about 25 ppm to about 225 ppm,

about 25 ppm to about 250 ppm, about 25 ppm to about 275 ppm, about 25 ppm to about 300 ppm, about

25 ppm to about 325 ppm, about 25 ppm to about 350 ppm, about 25 ppm to about 375 ppm, about 25 ppm

to about 400 ppm, about 50 ppm to about 75 ppm, about 50 ppm to about 100 ppm, about 50 ppm to about

125 ppm, about 50 ppm to about 150 ppm, about 50 ppm to about 175 ppm, about 50 ppm to about 200

ppm, about 50 ppm to about 225 ppm, about 50 ppm to about 250 ppm, about 50 ppm to about 275 ppm,

about 50 ppm to about 300 ppm, about 50 ppm to about 325 ppm, about 50 ppm to about 350 ppm, about

50 ppm to about 375 ppm, about 50 ppm to about 400 ppm, about 75 ppm to about 100 ppm, about 75 ppm

to about 125 ppm, about 75 ppm to about 150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to

about 200 ppm, about 75 ppm to about 225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about

275 ppm, about 75 ppm to about 300 ppm, about 75 ppm to about 325 ppm, about 75 ppm to about 350

ppm, about 75 ppm to about 375 ppm, about 75 ppm to about 400 ppm, about 100 ppm to about 125 ppm,

about 100 ppm to about 150 ppm, about 100 ppm to about 175 ppm, about 100 ppm to about 200 ppm,

about 100 ppm to about 225 ppm, about 100 ppm to about 250 ppm, about 100 ppm to about 275 ppm,

about 100 ppm to about 300 ppm, about 100 ppm to about 325 ppm, about 100 ppm to about 350 ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400 ppm, about 150 ppm to about 175 ppm, about 150 ppm to about 200 ppm, about 150 ppm to about 225 ppm, about 150 ppm to about 250 ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300 ppm, about 150 ppm to about 325 ppm, about 150 ppm to about 350 ppm, about 150 ppm to about 375 ppm, about 150 ppm to about 400 ppm, about 200 ppm to about 225 ppm, about 200 ppm to about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400 ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300 ppm, about 250 ppm to about 325 ppm, about 250 ppm to about 350 ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400 ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, or about 375 ppm to about 400 ppm.

[076] In other aspects of this embodiment, a composition disclosed herein comprises a quaternary

compound or silicon quaternary compound in an amount of, e.g., about 400 ppm to about 500 ppm, about

400 ppm to about 600 ppm, about 400 ppm to about 700 ppm, about 400 ppm to about 800 ppm, about

400 ppm to about 900 ppm, about 400 ppm to about 1,000 ppm, about 400 ppm to about 1,100 ppm, about

400 ppm to about 1,200 ppm, about 400 ppm to about 1,300 ppm, about 400 ppm to about 1,400 ppm,

about 400 ppm to about 1,500 ppm, about 400 ppm to about 1,600 ppm, about 400 ppm to about 1,700

ppm, about 400 ppm to about 1,800 ppm, about 400 ppm to about 1,900 ppm, about 400 ppm to about

2,000 ppm, about 500 ppm to about 600 ppm, about 500 ppm to about 700 ppm, about 500 ppm to about

800 ppm, about 500 ppm to about 900 ppm, about 500 ppm to about 1,000 ppm, about 500 ppm to about

1,100 ppm, about 500 ppm to about 1,200 ppm, about 500 ppm to about 1,300 ppm, about 500 ppm to

about 1,400 ppm, about 500 ppm to about 1,500 ppm, about 500 ppm to about 1,600 ppm, about 500 ppm

to about 1,700 ppm, about 500 ppm to about 1,800 ppm, about 500 ppm to about 1,900 ppm, about 500

ppm to about 2,000 ppm, about 600 ppm to about 700 ppm, about 600 ppm to about 800 ppm, about 600

ppm to about 900 ppm, about 600 ppm to about 1,000 ppm, about 600 ppm to about 1,100 ppm, about 600

ppm to about 1,200 ppm, about 600 ppm to about 1,300 ppm, about 600 ppm to about 1,400 ppm, about

600 ppm to about 1,500 ppm, about 600 ppm to about 1,600 ppm, about 600 ppm to about 1,700 ppm,

about 600 ppm to about 1,800 ppm, about 600 ppm to about 1,900 ppm, about 600 ppm to about 2,000

ppm, about 700 ppm to about 800 ppm, about 700 ppm to about 900 ppm, about 700 ppm to about 1,000

ppm, about 700 ppm to about 1,100 ppm, about 700 ppm to about 1,200 ppm, about 700 ppm to about

1,300 ppm, about 700 ppm to about 1,400 ppm, about 700 ppm to about 1,500 ppm, about 700 ppm to

about 1,600 ppm, about 700 ppm to about 1,700 ppm, about 700 ppm to about 1,800 ppm, about 700 ppm

to about 1,900 ppm, about 700 ppm to about 2,000 ppm, about 800 ppm to about 900 ppm, about 800 ppm

to about 1,000 ppm, about 800 ppm to about 1,100 ppm, about 800 ppm to about 1,200 ppm, about 800

ppm to about 1,300 ppm, about 800 ppm to about 1,400 ppm, about 800 ppm to about 1,500 ppm, about

800 ppm to about 1,600 ppm, about 800 ppm to about 1,700 ppm, about 800 ppm to about 1,800 ppm,

about 800 ppm to about 1,900 ppm, about 800 ppm to about 2,000 ppm, about 900 ppm to about 1,000

ppm, about 900 ppm to about 1,100 ppm, about 900 ppm to about 1,200 ppm, about 900 ppm to about

1,300 ppm, about 900 ppm to about 1,400 ppm, about 900 ppm to about 1,500 ppm, about 900 ppm to

about 1,600 ppm, about 900 ppm to about 1,700 ppm, about 900 ppm to about 1,800 ppm, about 900 ppm to about 1,900 ppm, about 900 ppm to about 2,000 ppm, about 1,000 ppm to about 1, 100 ppm, about 1,000 ppm to about 1,200 ppm, about 1,000 ppm to about 1,300 ppm, about 1,000 ppm to about 1,400 ppm, about 1,000 ppm to about 1,500 ppm, about 1,000 ppm to about 1,600 ppm, about 1,000 ppm to about

1,700 ppm, about 1,000 ppm to about 1,800 ppm, about 1,000 ppm to about 1,900 ppm, about 1,000 ppm

to about 2,000 ppm, about 1,100 ppm to about 1,200 ppm, about 1,100 ppm to about 1,300 ppm, about

1,100 ppm to about 1,400 ppm, about 1,100 ppm to about 1,500 ppm, about 1,100 ppm to about 1,600

ppm, about 1,100 ppm to about 1,700 ppm, about 1,100 ppm to about 1,800 ppm, about 1,100 ppm to

about 1,900 ppm, about 1,100 ppm to about 2,000 ppm, about 1,200 ppm to about 1,300 ppm, about 1,200

ppm to about 1,400 ppm, about 1,200 ppm to about 1,500 ppm, about 1,200 ppm to about 1,600 ppm,

about 1,200 ppm to about 1,700 ppm, about 1,200 ppm to about 1,800 ppm, about 1,200 ppm to about

1,900 ppm, about 1,200 ppm to about 2,000 ppm, about 1,300 ppm to about 1,400 ppm, about 1,300 ppm

to about 1,500 ppm, about 1,300 ppm to about 1,600 ppm, about 1,300 ppm to about 1,700 ppm, about

1,300 ppm to about 1,800 ppm, about 1,300 ppm to about 1,900 ppm, about 1,300 ppm to about 2,000

ppm, about 1,400 ppm to about 1,500 ppm, about 1,400 ppm to about 1,600 ppm, about 1,400 ppm to

about 1,700 ppm, about 1,400 ppm to about 1,800 ppm, about 1,400 ppm to about 1,900 ppm, about 1,400

ppm to about 2,000 ppm, about 1,500 ppm to about 1,600 ppm, about 1,500 ppm to about 1,700 ppm,

about 1,500 ppm to about 1,800 ppm, about 1,500 ppm to about 1,900 ppm, about 1,500 ppm to about

2,000 ppm, about 1,600 ppm to about 1,700 ppm, about 1,600 ppm to about 1,800 ppm, about 1,600 ppm

to about 1,900 ppm, about 1,600 ppm to about 2,000 ppm, about 1,700 ppm to about 1,800 ppm, about

1,700 ppm to about 1,900 ppm, about 1,700 ppm to about 2,000 ppm, about 1,800 ppm to about 1,900

ppm, about 1,800 ppm to about 2,000 ppm, or about 1,900 ppm to about 2,000 ppm.

[077] In aspects of this embodiment, a composition disclosed herein comprises a quaternary compound

or silicon quaternary compound in an amount of, e.g., about 0.001%, about 0.005%, about 0.01%, about

0.05%, about 0.10%, about 0.11%, about 0.12%, about 0.13%, about 0.14%, about 0.15%, about 0.16%,

about 0.17%, about 0.18%, about 0.19%, about 0.2%, about 0.25%, about 0.3%, about 0.35%, about 0.4%,

about 0.45%, or about 0.5% by weight of the composition. In other aspects of this embodiment, a

composition disclosed herein comprises a quaternary compound or silicon quaternary compound in an

amount of, e.g., at least 0.001%, at least 0.005%, at least 0.01%, at least 0.05%, at least 0.10%, at least

0.11%, at least 0.12%, at least 0.13%, at least 0.14%, at least 0.15%, at least 0.16%, at least 0.17%, at

least 0.18%, at least 0.19%, at least 0.2%, at least 0.25%, at least 0.3%, at least 0.35%, at least 0.4%, at

least 0.45%, or at least 0.5% by weight of the composition. In yet other aspects of this embodiment, a

amount of, e.g., at most 0.001%, at most 0.005%, at most 0.01%, at most 0.05%, at most 0.10%, at most

0.11%, at most 0.12%, at most 0.13%, at most 0.14%, at most 0.15%, at most 0.16%, at most 0.17%, at

most 0.18%, at most 0.19%, at most 0.2%, at most 0.25%, at most 0.3%, at most 0.35%, at most 0.4%, at

most 0.45%, or at most 0.5% by weight of the composition. In still other aspects of this embodiment, a

amount of, e.g., about 0.0010% to about 0.005%, about 0.0010% to about 0.010%, about 0.0010% to about

0.050%, about 0.0010% to about 0.10%, about 0.0010% to about 0.15%, about 0.0010% to about 0.20%,

about 0.0010% to about 0.25%, about 0.0010% to about 0.30%, about 0.0010% to about 0.35%, about

0.0010% to about 0.40%, about 0.0010% to about 0.45%, about 0.0010% to about 0.50%, about 0.010%

to about 0.050%, about 0.010% to about 0.10%, about 0.010% to about 0.15%, about 0.010% to about

0.20%, about 0.010% to about 0.25%, about 0.010% to about 0.30%, about 0.010% to about 0.35%, about

0.010% to about 0.40%, about 0.010% to about 0.45%, about 0.010% to about 0.50%, about 0.10% to

about 0.15%, about 0.10% to about 0.20%, about 0.10% to about 0.25%, about 0.10% to about 0.30%,

about 0.10% to about 0.35%, about 0.10% to about 0.40%, about 0.10% to about 0.45%, about 0.10% to

about 0.50%, about 0.20% to about 0.25%, about 0.20% to about 0.30%, about 0.20% to about 0.35%,

about 0.20% to about 0.40%, about 0.20% to about 0.45%, about 0.20% to about 0.50%, about 0.30% to

about 0.35%, about 0,30% to about 0.40%, about 0.30% to about 0.45%, about 0.30% to about 0.50%,

about 0.40% to about 0.45%, about 0.40% to about 0.50%, or about 0.40% to about 0.50% by weight of

the composition.

[078] In aspects of this embodiment, a composition disclosed herein comprises a quaternary compound

or silicon quaternary compound in an amount of, e.g., about 1.0%, about 2.0%, about 3.0%, about 4.0%,

about 5.0%, about 6.0%, about 7.0%, about 8.0%, about 9.0%, about 10%, about 11%, about 12%, about

13%, about 14%, or about 15% by weight of the composition. In other aspects of this embodiment, a

amount of, e.g., at least 1.0%, at least 2.0%, at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at

least 7.0%, at least 8.0%, at least 9.0%, at least 10%, at least 11%, at least 12%, at least 13%, at least

14%, or at least 15% by weight of the composition. In yet other aspects of this embodiment, a composition

disclosed herein comprises a quaternary compound or silicon quaternary compound in an amount of, e.g.,

at most 1.0%, at most 2.0%, at most 3.0%, at most 4.0%, at most 5.0%, at most 6.0%, at most 7.0%, at

most 8.0%, at most 9.0%, at most 10%, at most 11%, at most 12%, at most 13%, at most 14%, or at most

15% by weight of the composition. In still other aspects of this embodiment, a composition disclosed herein

comprises a quaternary compound or silicon quaternary compound in an amount of, e.g., about 1.0% to

about 2.0%, about 1.0% to about 3.0%, about 1.0% to about 4.0%, about 1.0% to about 5.0%, about 1.0%

to about 6.0%, about 1.0% to about 7.0%, about 1.0% to about 8.0%, about 1.0% to about 9.0%, about

1.0% to about 10%, about 1.0% to about 11%, about 1.0% to about 12%, about 1.0% to about 13%, about

1.0% to about 14%, about 1.0% to about 15%, about 2.0% to about 3.0%, about 2.0% to about 4.0%, about

2.0% to about 5.0%, about 2.0% to about 6.0%, about 2.0% to about 7.0%, about 2.0% to about 8.0%,

about 2.0% to about 9.0%, about 2.0% to about 10%, about 2.0% to about 11%, about 2.0% to about 12%,

about 2.0% to about 13%, about 2.0% to about 14%, about 2.0% to about 15%, about 3.0% to about 4.0%,

about 3.0% to about 5.0%, about 3.0% to about 6.0%, about 3.0% to about 7.0%, about 3.0% to about

8.0%, about 3.0% to about 9.0%, about 3.0% to about 10%, about 3.0% to about 11%, about 3.0% to about

12%, about 3.0% to about 13%, about 3.0% to about 14%, about 3.0% to about 15%, about 4.0% to about

5.0%, about 4.0% to about 6.0%, about 4.0% to about 7.0%, about 4.0% to about 8.0%, about 4.0% to

about 9.0%, about 4.0% to about 10%, about 4.0% to about 11%, about 4.0% to about 12%, about 4.0% to

about 13%, about 4.0% to about 14%, about 4.0% to about 15%, about 5.0% to about 6.0%, about 5.0% to

about 7.0%, about 5.0% to about 8.0%, about 5.0% to about 9.0%, about 5.0% to about 10%, about 5.0%

to about 11%, about 5.0% to about 12%, about 5.0% to about 13%, about 5.0% to about 14%, about 5.0%

to about 15%, about 6.0% to about 7.0%, about 6.0% to about 8.0%, about 6.0% to about 9.0%, about

6.0% to about 10%, about 6.0% to about 11%, about 6.0% to about 12%, about 6.0% to about 13%, about

6.0% to about 14%, about 6.0% to about 15%, about 7.0% to about 8.0%, about 7.0% to about 9.0%, about

7.0% to about 10%, about 7.0% to about 11%, about 7.0% to about 12%, about 7.0% to about 13%, about

7.0% to about 14%, about 7.0% to about 15%, about 8.0% to about 9.0%, about 8.0% to about 10%, about

8.0% to about 11%, about 8.0% to about 12%, about 8.0% to about 13%, about 8.0% to about 14%, about

8.0% to about 15%, about 9.0% to about 10%, about 9.0% to about 11%, about 9.0% to about 12%, about

9.0% to about 13%, about 9.0% to about 14%, about 9.0% to about 15%, about 10% to about 11%, about

10% to about 12%, about 10% to about 13%, about 10% to about 14%, about 10% to about 15%, about

11% to about 12%, about 11% to about 13%, about 11% to about 14%, about 11% to about 15%, about

12% to about 13%, about 12% to about 14%, about 12% to about 15%, about 13% to about 14%, about

13% to about 15%, or 14% to about 15% by weight of the composition.

[079] In an embodiment, a composition disclosed herein does not comprise a quaternary compound. In

an embodiment, a composition disclosed herein does not comprise a silicon quaternary compound. In an

embodiment, a composition disclosed herein does not comprise a quaternary compound or a silicon

quaternary compound.

[080] A composition disclosed herein may comprise a compound containing a guanide moiety and/or

functional group. Non-limiting examples of a guanide-containing compound include an organic compound

containing a biguanide functional group, a biguanidine functional group or a triguanide functional group. In

one embodiment, a guanide-containing compound disclosed herein is a biguanide or a biguanide-

containing compound. In an aspect of this embodiment, a biguanide-containing compound comprises one,

two, three, four or five biguanide functional groups. In an aspect of this embodiment, a biguanide-containing

compound comprises formula II,

NH NH R5 N N H H H wherein R5 and R6 can each independently be a bond, H, C, NH, NH², a C1-10 alkyl, a C1-10 alkane, a C1-10

alkyne, a 5 or 6 carbon aromatic ring, optionally substituted with a halogen; and n is 1-5. A halogen is F,

Br, CI, I, and At. Non-limiting examples of a biguanide-containing compound include a polyhexamethylene

biguanide (PHMB), a polyaminopropyl biguanide (PAPB), a 1,1'-(1,6-Hexanediyl)bis{2-[N'-(2- ethylhexyl)carbamimidoyl]guanidine} (alexidine), a chlorhexidine and a chlorhexidine gluconate.

[081] In one embodiment, a guanide-containing compound disclosed herein is a biguanidine or a

biguanidine-containing compound. In an aspect of this embodiment, a biguanidine-containing compound

comprises one, two, three, four or five biguanidine functional groups. In an aspect of this embodiment, a

biguanidine-containing compound comprises formula III,

PCT/US2020/037665 35

NH NH H N R7 R N N R°8 H H n III

NH wherein R7 and R° can each independently be a bond, H, C, NH, NH², a C1-10 alkyl, a C1-10 alkane, a C1-10

alkyne, a 5 or 6 carbon aromatic ring, optionally substituted with a halogen; and in is 1-5. A halogen is F,

Br, CI, I, and At.

[082] In one embodiment, a guanide-containing compound disclosed herein is a triguanide or a

triguanide-containing compound. In an aspect of this embodiment, a triguanide-containing compound

comprises one, two, three, four or five triguanide functional groups. In an aspect of this embodiment, a

triguanide-containing compound comprises formula IV,

NH NH NH 10 R10 R9 N N R H H H H n IV

wherein R° and R10 can each independently be a bond, H, C, NH, NH², a C1-1o alkyl, a C1-10 alkane, a C1-10

Br, CI, I, and At.

[083] In one embodiment, a single guanide-containing compound is present in a composition disclosed

herein. In another embodiment, a plurality of guanide-containing compounds is present in a composition

disclosed herein. In aspects of this embodiment, a composition disclosed herein comprises, e.g., one or

more guanide-containing compounds, two or more guanide-containing compounds, three or more guanide-

containing compounds, four or more guanide-containing compounds or five or more guanide-containing

compounds. In other aspects of this embodiment, a composition disclosed herein comprises, e.g., only

one guanide-containing compound, at most two guanide-containing compounds, at most three guanide-

containing compounds, at most four guanide-containing compounds, or at most five guanide-containing

compounds. In yet other aspects of this embodiment, a composition disclosed herein comprises from, e.g.,

1 to 2 guanide-containing compounds, 1 to 3 guanide-containing compounds, 1 to 4 guanide-containing

compounds, 1 to 5 guanide-containing compounds, 2 to 3 guanide-containing compounds, 2 to 4 guanide-

containing compounds, 2 to 5 guanide-containing compounds, 3 to 4 guanide-containing compounds, 3 to

5 guanide-containing compounds or 4 to 5 guanide-containing compounds.

[084] Any amount of a guanide-containing compound disclosed herein may be used, with the proviso

that the amount is useful to practice the methods disclosed herein. For example, when a composition

comprising a guanide-containing compound is formulated to be used in an undiluted state, the amount of a

guanide-containing compound used is a microbiostatic effective amount. A microbiostatic effective amount

of a guanide-containing compound is an amount that restrains, prohibits, or otherwise prevents microbial

population growth without eradicating the microbes. In an aspect of this embodiment, a microbiostatic

effective amount of a guanide-containing compound is an amount that restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbes to a degree that achieves disinfection or sterilization. Alternatively, when a composition comprising a guanide-containing compound is formulated to be used in methods where the composition will be diluted, the amount of a guanide- containing compound used in such concentrated formulations can be any amount that does not cause precipitation of components in the composition disclosed herein or otherwise create unwanted effects that make the composition inoperable.

[085] In aspects of this embodiment, a composition disclosed herein comprises a guanide-containing

compound in an amount of, e.g., about 0.01%, about 0.05%, about 0.075%, about 0.1%, about 0.2%, about

0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1.5%,

about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about 6.0%, about 7.0%, about 7.5%, about

8.0%, about 9.0%, about 10.0%. about 11%, about 12%, about 13%, about 14%, about 15%, about 20%,

about 25%, or about 30% by weight of the composition. In other aspects of this embodiment, a composition

disclosed herein comprises a guanide-containing compound in an amount of, e.g., at least 0.01%, at least

0.05%, at least 0.075%, at least 0.1%, at least 0.25%, at least 0.5%, at least 0.75%, at least 1.0%, at least

1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at least 7.0%,

at least 7.5%, at least 8.0%, at least 9.0%, at least 10.0%, at least 11%, at least 12%, at least 13%, at least

14%, at least 15%, at least 20%, at least 25%, or at least 30% by weight of the composition. In yet other

aspects of this embodiment, a composition disclosed herein comprises a guanide-containing compound in

an amount of, e.g., at most 0.01%, at most 0.05%, at most 0.075%, at most 0.1%, at most 0.25%, at most

0.5%, at most 0.75%, at most 1.0%, at most 1.5%, at most 2.0%, at most 2.5%, at most 3.0%, at most

4.0%, at most 5.0%, at most 6.0%, at most 7.5%, at most 8.0%, at most 9.0%, at most 10.0%, at most 11%,

at most 12%, at most 13%, at most 14%, at most 15%, at most 20%, at most 25%, or at most 30% by

weight of the composition.

[086] In still other aspects of this embodiment, a composition disclosed herein comprises a guanide-

containing compound in an amount of, e.g., about 0.1% to about 0.5%, about 0.1% to about 0.75%, about

0.1% to about 1.0%, about 0.1% to about 1.5%, about 0.1% to about 2.0%, about 0.1% to about 2.5%,

about 0.1% to about 3.0%, about 0.1% to about 3.5%, about 0.1% to about 4.0%, about 0.1% to about

4.5%, about 0.1% to about 5.0%, about 0.2% to about 0.5%, about 0.2% to about 0.75%, about 0.2% to

about 1.0%, about 0.2% to about 1.5%, about 0.2% to about 2.0%, about 0.2% to about 2.5%, about 0.2%

to about 3.0%, about 0.2% to about 3.5%, about 0.2% to about 4.0%, about 0.2% to about 4.5%, about

0.2% to about 5.0%, about 0.5% to about 1.0%, about 0.5% to about 1.5%, about 0.5% to about 2.0%,

about 0.5% to about 2.5%, about 0.5% to about 3.0%, about 0.5% to about 4.0%, about 0.5% to about

5.0%, about 0.5% to about 6.0%, about 0.5% to about 7.0%, about 0.5% to about 8.0%, about 0.5% to

about 9.0%, about 0.5% to about 10.0%, about 1.0% to about 2.5%, about 1.0% to about 3.0%, about 1.0%

to about 4.0%, about 1.0% to about 5.0%, about 1.0% to about 6.0%, about 1.0% to about 7.0%, about

1.0% to about 7.5%, about 1.0% to about 8.0%, about 1.0% to about 9.0%, about 1.0% to about 10.0%,

about 1.0% to about 15.0%, about 1.0% to about 20.0%, about 1.0% to about 25.0%, about 1.0% to about

30.0%, about 2.0% to about 2.5%, about 2.0% to about 3.0%, about 2.0% to about 4.0%, about 2.0% to

about 5.0%, about 2.0% to about 6.0%, about 2.0% to about 7.0%, about 2.0% to about 7.5%, about 2.0% wo 2020/252433 WO PCT/US2020/037665 37 to about 8.0%, about 2.0% to about 9.0%, about 2.0% to about 10.0%, about 2.0% to about 15.0%, about

2.0% to about 20.0%, about 2.0% to about 25.0%, about 2.0% to about 30.0%, about 5.0% to about 6.0%,

about 5.0% to about 7.0%, about 5.0% to about 7.5%, about 5.0% to about 8.0%, about 5.0% to about

9.0%, about 5.0% to about 10.0%, about 5.0% to about 11.0%, about 5.0% to about 12.0%, about 5.0% to

about 13.0%, about 5.0% to about 14.0%, about 5.0% to about 15.0%, about 5.0% to about 20.0%, about

5.0% to about 25.0%, about 5.0% to about 30.0%, about 10.0% to about 15.0%, about 10.0% to about

20.0%, about 10.0% to about 25.0%, about 10.0% to about 30.0%, about 15.0% to about 20.0%, about

15.0% to about 25.0%, about 15.0% to about 30.0%, about 20.0% to about 25.0%, about 20.0% to about

30.0%, or about 20.0% to about 30.0%, by weight of the composition.

[087] In another embodiment, a composition disclosed herein does not comprise a guanide-containing

compound. In another embodiment, a composition disclosed herein does not comprise biguanide. In

another embodiment, a composition disclosed herein does not comprise a biguanide-containing compound.

In another embodiment, a composition disclosed herein does not comprise biguanidine. In another

embodiment, a composition disclosed herein does not comprise a biguanidine-containing compound. In

another embodiment, a composition disclosed herein does not comprise triguanide. In another embodiment, a composition disclosed herein does not comprise a triguanide-containing compound.

[088] A composition disclosed herein may comprise an alcohol. An alcohol is an organic molecule

comprising a hydroxyl functional group (-OH) bond to a carbon atom, where the carbon atom is saturated.

In aspects of this embodiment, the alcohol may be, e.g., a C2-4 alcohol, a C1-4 alcohol, a C1-5 alcohol, a C1-

7 alcohol, a C1-10 alcohol, a C1-15 alcohol, or a C1-20 alcohol. In other aspects of this embodiment, an alcohol

may be, e.g., a primary alcohol, a secondary alcohol, or a tertiary alcohol. In other aspects of this

embodiment, an alcohol may be, e.g., an acyclic alcohol, a monohydric alcohol, a polyhydric alcohol (also

known as a polyol or sugar alcohol), an unsaturated aliphatic alcohol, an alicyclic alcohol, or a combination

thereof. Examples of a monohydric alcohol include, without limitation, methanol, ethanol, propanol,

isopropanol, butanol, pentanol, and 1-hexadecanol. Examples of a polyhydric alcohol include, without

limitation, glycol, glycerol, arabitol, erythritol, xylitol, maltitol, sorbitol (gluctiol), mannitol, inositol, lactitol,

galactitol (iditol), and isomalt. Examples of an unsaturated aliphatic alcohol include, without limitation, prop-

2-ene-1-ol, 3,7-dimethylocta-2,6-dien-1-ol, and prop-2-in-1-ol. Examples of an alicyclic alcohol include,

without limitation, cyclohexane-1,2,3,4,5,6-hexol and 2 - (2-propyl)-5-methyl-cyclohexane-1-ol

[089] In one embodiment, a single alcohol is present in a composition disclosed herein. In another

embodiment, a plurality of alcohols is present in a composition disclosed herein. In aspects of this

embodiment, a composition disclosed herein comprises, e.g., one or more alcohols, two or more alcohols,

three or more alcohols, four or more alcohols or five or more alcohols. In other aspects of this embodiment,

a composition disclosed herein comprises, e.g., only one alcohol, at most two alcohols, at most three

alcohols, at most four alcohols, or at most five alcohols. In yet other aspects of this embodiment, a

composition disclosed herein comprises from, e.g., 1 to 2 alcohols, 1 to 3 alcohols, 1 to 4 alcohols, 1 to 5

alcohols, 2 to 3 alcohols, 2 to 4 alcohols, 2 to 5 alcohols, 3 to 4 alcohols, 3 to 5 alcohols or 4 to 5 alcohols.

[090] In aspects of this embodiment, a plurality of alcohols is present in a composition disclosed herein

may comprise, e.g., about 99% of a first alcohol and about 1% of a second alcohol, about 98% of a first

alcohol and about 2% of a second alcohol, about 97% of a first alcohol and about 3% of a second alcohol,

about 96% of a first alcohol and about 4% of a second alcohol, about 95% of a first alcohol and about 5%

of a second alcohol, about 90% of a first alcohol and about 10% of a second alcohol, about 80% of a first

alcohol and about 20% of a second alcohol, about 70% of a first alcohol and about 30% of a second alcohol,

about 60% of a first alcohol and about 40% of a second alcohol, about 50% of a first alcohol and about

50% of a second alcohol, about 40% of a first alcohol and about 60% of a second alcohol, about 30% of a

first alcohol and about 70% of a second alcohol, about 20% of a first alcohol and about 80% of a second

alcohol, about 10% of a first alcohol and about 90% of a second alcohol, about 5% of a first alcohol and

about 95% of a second alcohol. In other aspects of this embodiment, the ratio of a first alcohol to a second

alcohol is, e.g., 100:1, 90:1, 80:1, 70:1, 60:1, 50:1, 40:1, 30:1, 20:1, 19:1, 18:1, 17:1, 16:1, 15:1, 14:1, 13:1,

12:1, 11:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11,

1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:80, 1:90, or 1:100. In

other aspects, the first alcohol is isopropanol and the second alcohol is ethanol.

[091] Any amount of an alcohol disclosed herein may be used, with the proviso that the amount is useful

to practice the methods disclosed herein. For example, when a composition comprising an alcohol is

formulated to be used in an undiluted state, the amount of an alcohol used is a microbiostatic effective

amount. A microbiostatic effective amount of an alcohol is an amount that restrains, prohibits, or otherwise

prevents microbial population growth without eradicating the microbes. In an aspect of this embodiment, a

microbiostatic effective amount of an alcohol is an amount that restrains, prohibits, or otherwise prevents

sterilization. Alternatively, when a composition comprising an alcohol is formulated to be used in methods

where the composition will be diluted, the amount of an alcohol used in such concentrated formulations can

be any amount that does not cause precipitation of components in the composition disclosed herein or

otherwise create unwanted effects that make the composition inoperable.

[092] In aspects of this embodiment, a composition disclosed herein comprises an alcohol in an amount

of, e.g., about 0.5%, about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%,

about 9%, about 10%, about 11%, about 12%, about 13%, about 14%, about 15%, about 20%, about 25%,

about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about

70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 96%, about 97%, about 98%, or

about 99%, by weight of the composition. In other aspects of this embodiment, a composition disclosed

herein comprises an alcohol in an amount of, e.g., at least 0.5%, at least 1%, at least 2%, at least 3%, at

least 4%, at least 5%, at least 6%,atleast7%,atleast 8%,atleast9%,atleast 10%, at least 11%, at least

12%, at least 13%, at least 14%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at

least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%,

at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least

99%, by weight of the composition. In yet other aspects of this embodiment, a composition disclosed herein

comprises an alcohol in an amount of, e.g., at most 0.5%, at most 1%, at most 2%, at most 3%, at most

4%, at most 5%, at most 6%, at most 7%, at most 8%, at most 9%, at most 10%, at most 11%, at most

12%, at most 13%, at most 14%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at

most 40%, at most 45%, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%, at most 75%,

at most 80%, at most 85%, at most 90%, at most 95%, at most 96%, at most 97%, at most 98%, or at most

99%, by weight of the composition.

[093] In still other aspects of this embodiment, a composition disclosed herein comprises an alcohol in

an amount of, e.g., about 0.05% to about 0.75%, about 0.05% to about 1.0%, about 0.05% to about 1.5%,

about 0.05% to about 2.0%, about 0.05% to about 2.5%, about 0.1% to about 0.5%, about 0.1% to about

0.75%, about 0.1% to about 1.0%, about 0.1% to about 1.5%, about 0.1% to about 2.0%, about 0.1% to

about 2.5%, about 0.1% to about 3.0%, about 0.1% to about 4.0%, about 0.1% to about 5.0%, about 0.2%

to about 0.5%, about 0.2% to about 0.75%, about 0.2% to about 1.0%, about 0.2% to about 1.5%, about

0.2% to about 2.0%, about 0.2% to about 2.5%, about 0.2% to about 3.0%, about 0.2% to about 4.0%,

about 0.2% to about 5.0%, about 0.5% to about 1.0%, about 0.5% to about 1.5%, about 0.5% to about

2.0%, about 0.5% to about 2.5%, about 0.5% to about 3.0%, about 0.5% to about 4.0%, about 0.5% to

about 5.0%, about 0.5% to about 6.0%, about 0.5% to about 7.0%, about 0.5% to about 8.0%, about 0.5%

to about 9.0%, about 0.5% to about 10.0%, about 1.0% to about 2.5%, about 1.0% to about 3.0%, about

1.0% to about 4.0%, about 1.0% to about 5.0%, about 1.0% to about 6.0%, about 1.0% to about 7.0%,

about 1.0% to about 7.5%, about 1.0% to about 8.0%, about 1.0% to about 9.0%, about 1.0% to about

10.0%, about 2.0% to about 2.5%, about 2.0% to about 3.0%, about 2.0% to about 4.0%, about 2.0% to

about 5.0%, about 2.0% to about 6.0%, about 2.0% to about 7.0%, about 2.0% to about 7.5%, about 2.0%

to about 8.0%, about 2.0% to about 9.0%, about 2.0% to about 10.0%, about 5.0% to about 6.0%, about

5.0% to about 7.0%, about 5.0% to about 7.5%, about 5.0% to about 8.0%, about 5.0% to about 9.0%,

about 5.0% to about 10.0%, about 5.0% to about 11.0%, about 5.0% to about 12.0%, about 5.0% to about

13.0%, about 5.0% to about 14.0% or about 5.0% to about 15.0% by weight of the composition, or about

10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about

10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about

10% to about 95%, about 10% to about 97%, about 10% to about 99%, about 20% to about 30%, about

20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about

20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 20% to about 97%, about

20% to about 99%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about

30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about

30% to about 97%, about 30% to about 99%, about 40% to about 50%, about 40% to about 60%, about

40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about

40% to about 97%, about 40% to about 99%, about 50% to about 60%, about 50% to about 70%, about

50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 50% to about 97%, about

50% to about 99%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about

60% to about 95%, about 60% to about 97%, about 60% to about 99%, about 70% to about 80%, about

70% to about 90%, about 70% to about 95%, about 70% to about 97%, about 70% to about 99%, about

80% to about 90%, about 80% to about 95%, about 80% to about 97%, about 80% to about 99%, about

90% to about 95%, about 90% to about 97%, about 90% to about 99%, by weight of the composition.

wo 2020/252433 WO PCT/US2020/037665 40

[094] In aspects of this embodiment, a composition disclosed herein comprises a plurality of alcohols in

a total amount of, e.g., about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%,

about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about

85%, about 90%, about 95%, about 96%, about 97%, about 98%, or about 99%, by weight of the composition. In other aspects of this embodiment, a composition disclosed herein comprises a plurality of

alcohols in a total amount of, e.g., at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at

least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%,

at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least

98%, or at least 99%, by weight of the composition. In yet other aspects of this embodiment, a composition

disclosed herein comprises a plurality of alcohols in a total amount of, e.g., at most 10%, at most 15%, at

most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 40%, at most 45%, at most 50%,

at most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most

90%, at most 95%, at most 96%, at most 97%, at most 98%, or at most 99%, by weight of the composition.

In still other aspects of this embodiment, a composition disclosed herein comprises a plurality of alcohols

in a total amount of, e.g., about 10% to about 20%, about 10% to about 30%, about 10% to about 40%,

about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%,

about 10% to about 90%, about 10% to about 95%, about 10% to about 97%, about 10% to about 99%,

about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%,

about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%,

about 20% to about 97%, about 20% to about 99%, about 30% to about 40%, about 30% to about 50%,

about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%,

about 30% to about 95%, about 30% to about 97%, about 30% to about 99%, about 40% to about 50%,

about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%,

about 40% to about 95%, about 40% to about 97%, about 40% to about 99%, about 50% to about 60%,

about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%,

about 50% to about 97%, about 50% to about 99%, about 60% to about 70%, about 60% to about 80%,

about 60% to about 90%, about 60% to about 95%, about 60% to about 97%, about 60% to about 99%,

about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 70% to about 97%,

about 70% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 97%,

about 80% to about 99%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, by

weight of the composition.

[095] In another embodiment, a composition disclosed herein does not comprise an alcohol.

[096] A composition disclosed herein may comprise metallic particles. A metallic particle can be

composed of a single element, such as, e.g., copper, iron, silver, titanium or zinc or be a mixed metallic

particle composed of various combinations of different elements, such as, e.g., various combinations of two

or more of the following: copper, iron, silver, titanium or zinc. Non-limiting examples of a metallic particle

includes a copper particle, an iron particle, a potassium particle, a silver particle, a titanium particle, and a

zinc particle. Other non-limiting examples of a metallic particle include a metal acetate particle, a metal

WO wo 2020/252433 PCT/US2020/037665 41

chloride particle, a metal nitrate particle, or a metal oxide particle. A metal acetate particle includes, without

limitation, copper acetate, iron acetate, e.g. iron (II) acetate and iron (III) acetate, silver acetate, titanium

acetate, zinc acetate, or any combination thereof. A metal nitrate particle includes, without limitation,

copper nitrate, iron nitrate, e.g., iron (II) nitrate, iron (III) nitrate, silver nitrate, titanium nitrate, zinc nitrate,

or any combination thereof. A metal chloride particle includes, without limitation, copper chloride, iron

chloride, e.g., iron (II) chloride or iron (III) chloride, silver chloride, titanium chloride, zinc chloride, or any

combination thereof. A metal oxide particle includes, without limitation, copper oxide particle, iron oxide

particle, e.g., iron (II) oxide particle, iron (III) oxide particle, silver oxide particle, titanium dioxide particle,

zinc oxide particle, copper zinc iron oxide particle, or any combination thereof.

[097] A metallic particle includes nanoparticles and microparticles. A nanoparticle has an average

diameter of about 1 nm to about 1,000 nm. A microparticle has an average diameter of about 1 um to about

1,000 um.

[098] In an embodiment, a composition disclosed herein comprises an amount of metallic particles that

comprising metallic particles is formulated to be used in an undiluted state, the amount of metallic particles

used is a microbiostatic effective amount. A microbiostatic effective amount of metallic particles is an

amount that restrains, prohibits, or otherwise prevents microbial population growth without eradicating the

microbes. In an aspect of this embodiment, a microbiostatic effective amount of metallic particles is an

microbes to a degree that achieves disinfection or sterilization. Alternatively, when a composition

comprising metallic particles is formulated to be used in methods where the composition will be diluted, the

amount of metallic particles used in such concentrated formulations can be any amount that does not cause

precipitation of components in the composition disclosed herein or otherwise create unwanted effects that

make the composition inoperable.

[099] In aspects of this embodiment, a composition disclosed herein comprises metallic particles in an

amount of, e.g., 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm, 0.40 ppm, 0.45

ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80 ppm, 0.85 ppm, 0.90 ppm, 0.95

ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm, 50 ppm, 55

ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm, 95 ppm, 100 ppm, 125 ppm, 150 ppm,

175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm, 350 ppm, 375 ppm, 400 ppm, 425

ppm, 450 ppm, 475 ppm, 500 ppm, 525 ppm, 550 ppm, 575 ppm, 600 ppm, 625 ppm, 650 ppm, 675 ppm,

700 ppm, 725 ppm, 750 ppm, 775 ppm, 800 ppm, 825 ppm, 850 ppm, 875 ppm, 900 ppm, 925 ppm, 950

ppm, 975 ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm, 1075 ppm, 1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175

ppm, 1,200 ppm, 1,225 ppm, 1,250 ppm, 1,275 ppm, 1,300 ppm, 1,325 ppm, 1,350 ppm, 1,375 ppm, 1,400

ppm, 1,425 ppm, 1,450 ppm, 1,475 ppm, 1,500 ppm, 1,600 ppm, 1,700 ppm, 1,800 ppm, 1,900 ppm, or

2,000 ppm. In other aspects of this embodiment, a composition disclosed herein comprises metallic

particles in an amount of, e.g., at least 0.05 ppm, at least 0.10 ppm, at least 0.20 ppm, at least 0.30 ppm,

at least 0.40 ppm, at least 0.50 ppm, at least 0.60 ppm, at least 0.70 ppm, at least 0.80 ppm, at least 0.90 ppm, at least 1 ppm, at least 10 ppm, at least 20 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, at least 60 ppm, at least 70ppm,atleast 80 ppm,atleast90 ppm,atleast 100ppm,atleast 125 ppm, at least

150 ppm, at least 175 ppm, at least 200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at least

300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least

450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least

600 ppm, at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least

750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm, at least 850 ppm, at least 875 ppm, at least

900 ppm, at least 925 ppm, at least 950 ppm, at least 975 ppm, at least 1,000 ppm, at least 1,025 ppm, at

least 1,050 ppm, at least 1075 ppm, at least 1,100 ppm, at least 1,125 ppm, at least 1,150 ppm, at least

1,175 ppm, at least 1,200 ppm, at least 1,225 ppm, at least 1,250 ppm, at least 1,275 ppm, at least 1,300

ppm, at least 1,325 ppm, at least 1,350 ppm, at least 1,375 ppm, at least 1,400 ppm, at least 1,425 ppm,

at least 1,450 ppm, at least 1,475 ppm, at least 1,500 ppm, at least 1,600 ppm, at least 1,700 ppm, at least

1,800 ppm, at least 1,900 ppm, or at least 2,000 ppm. In yet other aspects of this embodiment, a

composition disclosed herein comprises metallic particles in an amount of, e.g., at most 0,05 ppm, at most

or at most 2,000 ppm.

[100] In still other aspects of this embodiment, a composition disclosed herein comprises metallic

particles in an amount of from, e.g., about 0.5 ppm to about 20 ppm, about 0.5 ppm to about 25 ppm, about

0.5 ppm to about 30 ppm, about 0.5 ppm to about 35 ppm, about 0.5 ppm to about 40 ppm, about 0.5 ppm

to about 45 ppm, about 0.5 ppm to about 50 ppm, about 0.5 ppm to about 55 ppm, about 0.5 ppm to about

60 ppm, about 0.5 ppm to about 65 ppm, about 0.5 ppm to about 70 ppm, about 0.5 ppm to about 75 ppm,

about 0.5 ppm to about 80 ppm, about 0.5 ppm to about 85 ppm, about 0.5 ppm to about 90 ppm, about

0.5 ppm to about 95 ppm, about 0.5 ppm to about 100 ppm, about 0.75 ppm to about 20 ppm, about 0.75

ppm to about 25 ppm, about 0.75 ppm to about 30 ppm, about 0.75 ppm to about 35 ppm, about 0.75 ppm

to about 40 ppm, about 0.75 ppm to about 45 ppm, about 0.75 ppm to about 50 ppm, about 0.75 ppm to

about 55 ppm, about 0.75 ppm to about 60 ppm, about 0.75 ppm to about 65 ppm, about 0.75 ppm to about

70 ppm, about 0.75 ppm to about 75 ppm, about 0.75 ppm to about 80 ppm, about 0.75 ppm to about 85 ppm, about 0.75 ppm to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75 ppm to about 100 ppm, about 1 ppm to about 20 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 30 ppm, about 1 ppm to about 35 ppm, about 1 ppm to about 40 ppm, about 1 ppm to about 45 ppm, about 1 ppm to about

50 ppm, about 1 ppm to about 55 ppm, about 1 ppm to about 60 ppm, about 1 ppm to about 65 ppm, about

1 ppm to about 70 ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 80 ppm, about 1 ppm to about

85 ppm, about 1 ppm to about 90 ppm, about 1 ppm to about 95 ppm, about 1 ppm to about 100 ppm,

85 ppm, about 5 ppm to about 90 ppm, about 5 ppm to about 95 ppm, about 5 ppm to about 100 ppm,

about 10 ppm to about 20 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 30 ppm, about 10

ppm to about 35 ppm, about 10 ppm to about 40 ppm, about 10 ppm to about 45 ppm, about 10 ppm to

about 50 ppm, about 10 ppm to about 55 ppm, about 10 ppm to about 60 ppm, about 10 ppm to about 65

ppm, about 10 ppm to about 70 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 80 ppm, about

to about 100 ppm.

[101] In other aspects of this embodiment, a composition disclosed herein comprises metallic particles

225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, about 75 ppm to about 300 ppm, about 75 ppm to about 325 ppm, about 75 ppm to about 350 ppm, about 75 ppm to about 375 ppm, about 75 ppm to about 400 ppm, about 100 ppm to about 125 ppm, about 100 ppm to about 150 ppm, about 100 ppm to about 175 ppm, about 100 ppm to about 200 ppm, about 100 ppm to about 225 ppm, about 100 ppm to about 250 ppm, about 100 ppm to about 275 ppm, about 100 ppm to about 300 ppm, about 100 ppm to about 325 ppm, about 100 ppm to about 350 ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400 ppm, about 150 ppm to about 175 ppm, about 150 ppm to about 200 ppm, about 150 ppm to about 225 ppm, about 150 ppm to about 250 ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300 ppm, about 150 ppm to about 325 ppm, about 150 ppm to about 350 ppm, about 150 ppm to about 375 ppm, about 150 ppm to about 400 ppm, about 200 ppm to about 225 ppm, about 200 ppm to about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400 ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300 ppm, about 250 ppm to about 325 ppm, about 250 ppm to about 350 ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400 ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, or about 375 ppm to about 400 ppm.

[102] In other aspects of this embodiment, a composition disclosed herein comprises metallic particles

about 1,400 ppm, about 800 ppm to about 1,500 ppm, about 800 ppm to about 1,600 ppm, about 800 ppm to about 1,700 ppm, about 800 ppm to about 1,800 ppm, about 800 ppm to about 1,900 ppm, about 800 ppm to about 2,000 ppm, about 900 ppm to about 1,000 ppm, about 900 ppm to about 1,100 ppm, about

about 1,900 ppm to about 2,000 ppm.

[103] In aspects of this embodiment, a composition disclosed herein comprises metallic particles in an

amount of, e.g., 0.05 mg/L, 0.10 mg/L, 0.15 mg/L, 0.20 mg/L, 0.25 mg/L, 0.30 mg/L, 0.35 mg/L, 0.40 mg/L,

0.45 mg/L, 0.50 mg/L, 0.55 mg/L, 0.60 mg/L, 0.65 mg/L, 0.70 mg/L, 0.75 mg/L, 0.80 mg/L, 0.85 mg/L, 0.90

mg/L, 0.95 mg/L, 1 mg/L, 5 mg/L, 10 mg/L, 15 mg/L, 20 mg/L, 25 mg/L, 30 mg/L, 35 mg/L, 40 mg/L, 45

mg/L, 50 mg/L, 55 mg/L, 60 mg/L, 65 mg/L, 70 mg/L, 75 mg/L, 80 mg/L, 85 mg/L, 90 mg/L, 95 mg/L, 100

mg/L, 125 mg/L, 150 mg/L, 175 mg/L, 200 mg/L, 225 mg/L, 250 mg/L, 275 mg/L, 300 mg/L, 325 mg/L, 350

mg/L, 375 mg/L, 400 mg/L, 425 mg/L, 450 mg/L, 475 mg/L, 500 mg/L, 525 mg/L, 550 mg/L, 575 mg/L, 600

mg/L, 625 mg/L, 650 mg/L, 675 mg/L, 700 mg/L, 725 mg/L, 750 mg/L, 775 mg/L, 800 mg/L, 825 mg/L, 850

mg/L, 875 mg/L, 900 mg/L, 925 mg/L, 950 mg/L, 975 mg/L, 1,000 mg/L, 1,025 mg/L, 1,050 mg/L, 1075

mg/L, 1,100 mg/L, 1,125 mg/L, 1,150 mg/L, 1,175 mg/L, 1,200 mg/L, 1,225 mg/L, 1,250 mg/L, 1,275 mg/L,

1,300 mg/L, 1,325 mg/L, 1,350 mg/L, 1,375 mg/L, 1,400 mg/L, 1,425 mg/L, 1,450 mg/L, 1,475 mg/L, 1,500

mg/L, 1,600 mg/L, 1,700 mg/L, 1,800 mg/L, 1,900 mg/L, or 2,000 mg/L. In other aspects of this

embodiment, a composition disclosed herein comprises metallic particles in an amount of, e.g., at least

0.05 mg/L, at least 0.10 mg/L, at least 0.20 mg/L, at least 0.30 mg/L, at least 0.40 mg/L, at least 0.50 mg/L,

WO wo 2020/252433 PCT/US2020/037665 46

at least 0.60 mg/L, at least 0.70 mg/L, at least 0.80 mg/L, at least 0.90 mg/L, at least 1 mg/L, at least 10

mg/L, at least 20 mg/L, at least 30 mg/L, at least 40 mg/L, at least 50 mg/L, at least 60 mg/L, at least 70

mg/L, at least 80 mg/L, at least 90 mg/L, at least 100 mg/L, at least 125 mg/L, at least 150 mg/L, at least

175 mg/L, at least 200 mg/L, at least 225 mg/L, at least 250 mg/L, at least 275 mg/L, at least 300 mg/L, at

least 325 mg/L, at least 350 mg/L, at least 375 mg/L, at least 400 mg/L, at least 425 mg/L, at least 450

mg/L, at least 475 mg/L, at least 500 mg/L, at least 525 mg/L, at least 550 mg/L, at least 575 mg/L, at least

600 mg/L, at least 625 mg/L, at least 650 mg/L, at least 675 mg/L, at least 700 mg/L, at least 725 mg/L, at

least 750 mg/L, at least 775 mg/L, at least 800 mg/L, at least 825 mg/L, at least 850 mg/L, at least 875

mg/L, at least 900 mg/L, at least 925 mg/L, at least 950 mg/L, at least 975 mg/L, at least 1,000 mg/L, at

least 1,025 mg/L, at least 1,050 mg/L, at least 1075 mg/L, at least 1,100 mg/L, at least 1,125 mg/L, at least

1,150 mg/L, at least 1,175 mg/L, at least 1,200 mg/L, at least 1,225 mg/L, at least 1,250 mg/L, at least

1,275 mg/L, at least 1,300 mg/L, at least 1,325 mg/L, at least 1,350 mg/L, at least 1,375 mg/L, at least

1,400 mg/L, at least 1,425 mg/L, at least 1,450 mg/L, at least 1,475 mg/L, at least 1,500 mg/L, at least

1,600 mg/L, at least 1,700 mg/L, at least 1,800 mg/L, at least 1,900 mg/L, or at least 2,000 mg/L. In yet

other aspects of this embodiment, a composition disclosed herein comprises metallic particles in an amount

of, e.g., at most 0.05 mg/L, at most 0.10 mg/L, at most 0.20 mg/L, at most 0.30 mg/L, at most 0.40 mg/L,

at most 0.50 mg/L, at most 0.60 mg/L, at most 0.70 mg/L, at most 0.80 mg/L, at most 0.90 mg/L, at most 1

mg/L, at most 10 mg/L, at most 20 mg/L, at most 30 mg/L, at most 40 mg/L, at most 50 mg/L, at most 60

mg/L, at most 70 mg/L, at most 80 mg/L, at most 90 mg/L, at most 100 mg/L, at most 125 mg/L, at most

150 mg/L, at most 175 mg/L, at most 200 mg/L, at most 225 mg/L, at most 250 mg/L, at most 275 mg/L, at

most 300 mg/L, at most 325 mg/L, at most 350 mg/L, at most 375 mg/L, at most 400 mg/L, at most 425

mg/L, at most 450 mg/L, at most 475 mg/L, at most 500 mg/L, at most 525 mg/L, at most 550 mg/L, at most

575 mg/L, at most 600 mg/L, at most 625 mg/L, at most 650 mg/L, at most 675 mg/L, at most 700 mg/L, at

most 725 mg/L, at most 750 mg/L, at most 775 mg/L, at most 800 mg/L, at most 825 mg/L, at most 850

mg/L, at most 875 mg/L, at most 900 mg/L, at most 925 mg/L, at most 950 mg/L, at most 975 mg/L, at most

1,000 mg/L, at most 1,025 mg/L, at most 1,050 mg/L, at most 1075 mg/L, at most 1,100 mg/L, at most

1,125 mg/L, at most 1,150 mg/L, at most 1,175 mg/L, at most 1,200 mg/L, at most 1,225 mg/L, at most

1,250 mg/L, at most 1,275 mg/L, at most 1,300 mg/L, at most 1,325 mg/L, at most 1,350 mg/L, at most

1,375 mg/L, at most 1,400 mg/L, at most 1,425 mg/L, at most 1,450 mg/L, at most 1,475 mg/L, at most

1,500 mg/L, at most 1,600 mg/L, at most 1,700 mg/L, at most 1,800 mg/L, at most 1,900 mg/L, or at most

2,000 mg/L.

[104] In still other aspects of this embodiment, a composition disclosed herein comprises metallic

particles in an amount of from, e.g., about 0.5 mg/L to about 20 mg/L, about 0.5 mg/L to about 25 mg/L,

about 0.5 mg/L to about 30 mg/L, about 0.5 mg/L to about 35 mg/L, about 0.5 mg/L to about 40 mg/L, about

0.5 mg/L to about 45 mg/L, about 0.5 mg/L to about 50 mg/L, about 0.5 mg/L to about 55 mg/L, about 0.5

mg/L to about 60 mg/L, about 0.5 mg/L to about 65 mg/L, about 0.5 mg/L to about 70 mg/L, about 0.5 mg/L

to about 75 mg/L, about 0.5 mg/L to about 80 mg/L, about 0.5 mg/L to about 85 mg/L, about 0.5 mg/L to

about 90 mg/L, about 0.5 mg/L to about 95 mg/L, about 0.5 mg/L to about 100 mg/L, about 0.75 mg/L to

about 20 mg/L, about 0.75 mg/L to about 25 mg/L, about 0.75 mg/L to about 30 mg/L, about 0.75 mg/L to

about 35 mg/L, about 0.75 mg/L to about 40 mg/L, about 0.75 mg/L to about 45 mg/L, about 0.75 mg/L to about 50 mg/L, about 0.75 mg/L to about 55 mg/L, about 0.75 mg/L to about 60 mg/L, about 0.75 mg/L to about 65 mg/L, about 0.75 mg/L to about 70 mg/L, about 0.75 mg/L to about 75 mg/L, about 0.75 mg/L to about 80 mg/L, about 0.75 mg/L to about 85 mg/L, about 0.75 mg/L to about 90 mg/L, about 0.75 mg/L to about 95 mg/L, about 0.75 mg/L to about 100 mg/L, about 1 mg/L to about 20 mg/L, about 1 mg/L to about

25 mg/L, about 1 mg/L to about 30 mg/L, about 1 mg/L to about 35 mg/L, about 1 mg/L to about 40 mg/L,

about 1 mg/L to about 45 mg/L, about 1 mg/L to about 50 mg/L, about 1 mg/L to about 55 mg/L, about 1

mg/L to about 60 mg/L, about 1 mg/L to about 65 mg/L, about 1 mg/L to about 70 mg/L, about 1 mg/L to

about 75 mg/L, about 1 mg/L to about 80 mg/L, about 1 mg/L to about 85 mg/L, about 1 mg/L to about 90

mg/L, about 1 mg/L to about 95 mg/L, about 1 mg/L to about 100 mg/L, about 5 mg/L to about 20 mg/L,

about 5 mg/L to about 25 mg/L, about 5 mg/L to about 30 mg/L, about 5 mg/L to about 35 mg/L, about 5

mg/L to about 40 mg/L, about 5 mg/L to about 45 mg/L, about 5 mg/L to about 50 mg/L, about 5 mg/L to

about 55 mg/L, about 5 mg/L to about 60 mg/L, about 5 mg/L to about 65 mg/L, about 5 mg/L to about 70

mg/L, about 5 mg/L to about 75 mg/L, about 5 mg/L to about 80 mg/L, about 5 mg/L to about 85 mg/L,

about 5 mg/L to about 90 mg/L, about 5 mg/L to about 95 mg/L, about 5 mg/L to about 100 mg/L, about 10

mg/L to about 20 mg/L, about 10 mg/L to about 25 mg/L, about 10 mg/L to about 30 mg/L, about 10 mg/L

to about 35 mg/L, about 10 mg/L to about 40 mg/L, about 10 mg/L to about 45 mg/L, about 10 mg/L to

about 50 mg/L, about 10 mg/L to about 55 mg/L, about 10 mg/L to about 60 mg/L, about 10 mg/L to about

65 mg/L, about 10 mg/L to about 70 mg/L, about 10 mg/L to about 75 mg/L, about 10 mg/L to about 80

mg/L, about 10 mg/L to about 85 mg/L, about 10 mg/L to about 90 mg/L, about 10 mg/L to about 95 mg/L,

or about 10 mg/L to about 100 mg/L.

[105] In other aspects of this embodiment, a composition disclosed herein comprises metallic particles

in an amount of from, e.g., about 1 mg/L to about 25 mg/L, about 1 mg/L to about 50 mg/L, about 1 mg/L

to about 75 mg/L, about 1 mg/l to about 100 mg/L, about 1 mg/L to about 125 mg/L, about 1 mg/L to about

150 mg/L, about 1 mg/L to about 175 mg/L, about 1 mg/L to about 200 mg/L, about 1 mg/L to about 225

mg/L, about 1 mg/L to about 250 mg/L, about 1 mg/L to about 275 mg/L, about 1 mg/L to about 300 mg/L,

about 1 mg/L to about 325 mg/L, about 1 mg/L to about 350 mg/L, about 1 mg/L to about 375 mg/L, about

1 mg/L to about 400 mg/L, about 10 mg/L to about 25 mg/L, about 10 mg/L to about 50 mg/L, about 10

mg/L to about 75 mg/L, about 10 mg/L to about 100 mg/L, about 10 mg/L to about 125 mg/L, about 10 mg/L

to about 150 mg/L, about 10 mg/L to about 175 mg/L, about 10 mg/L to about 200 mg/L, about 10 mg/L to

about 225 mg/L, about 10 mg/L to about 250 mg/L, about 10 mg/L to about 275 mg/L, about 10 mg/L to

about 300 mg/L, about 10 mg/L to about 325 mg/L, about 10 mg/L to about 350 mg/L, about 10 mg/L to

about 375 mg/L, about 10 mg/L to about 400 mg/L, about 25 mg/L to about 50 mg/L, about 25 mg/L to

about 75 mg/L, about 25 mg/L to about 100 mg/L, about 25 mg/L to about 125 mg/L, about 25 mg/L to

about 150 mg/L, about 25 mg/L to about 175 mg/L, about 25 mg/L to about 200 mg/L, about 25 mg/L to

about 225 mg/L, about 25 mg/L to about 250 mg/L, about 25 mg/L to about 275 mg/L, about 25 mg/L to

about 300 mg/L, about 25 mg/L to about 325 mg/L, about 25 mg/L to about 350 mg/L, about 25 mg/L to

about 375 mg/L, about 25 mg/L to about 400 mg/L, about 50 mg/L to about 75 mg/L, about 50 mg/L to

about 100 mg/L, about 50 mg/L to about 125 mg/L, about 50 mg/L to about 150 mg/L, about 50 mg/L to

about 175 mg/L, about 50 mg/L to about 200 mg/L, about 50 mg/L to about 225 mg/L, about 50 mg/L to

about 250 mg/L, about 50 mg/L to about 275 mg/L, about 50 mg/L to about 300 mg/L, about 50 mg/L to

PCT/US2020/037665 48

about 325 mg/L, about 50 mg/L to about 350 mg/L, about 50 mg/L to about 375 mg/L, about 50 mg/L to

about 400 mg/L, about 75 mg/L to about 100 mg/L, about 75 mg/L to about 125 mg/L, about 75 mg/L to

about 150 mg/L, about 75 mg/L to about 175 mg/L, about 75 mg/L to about 200 mg/L, about 75 mg/L to

about 225 mg/L, about 75 mg/L to about 250 mg/L, about 75 mg/L to about 275 mg/L, about 75 mg/L to

about 300 mg/L, about 75 mg/L to about 325 mg/L, about 75 mg/L to about 350 mg/L, about 75 mg/L to

about 375 mg/L, about 75 mg/L to about 400 mg/L, about 100 mg/L to about 125 mg/L, about 100 mg/L to

about 150 mg/L, about 100 mg/L to about 175 mg/L, about 100 mg/L to about 200 mg/L, about 100 mg/L

to about 225 mg/L, about 100 mg/L to about 250 mg/L, about 100 mg/L to about 275 mg/L, about 100 mg/L

to about 300 mg/L, about 100 mg/L to about 325 mg/L, about 100 mg/L to about 350 mg/L, about 100 mg/L

to about 375 mg/L, about 100 mg/L to about 400 mg/L, about 150 mg/L to about 175 mg/L, about 150 mg/L

to about 200 mg/L, about 150 mg/L to about 225 mg/L, about 150 mg/L to about 250 mg/L, about 150 mg/L

to about 275 mg/L, about 150 mg/L to about 300 mg/L, about 150 mg/L to about 325 mg/L, about 150 mg/L

to about 350 mg/L, about 150 mg/L to about 375 mg/L, about 150 mg/L to about 400 mg/L, about 200 mg/L

to about 225 mg/L, about 200 mg/L to about 250 mg/L, about 200 mg/L to about 275 mg/L, about 200 mg/L

to about 300 mg/L, about 200 mg/L to about 325 mg/L, about 200 mg/L to about 350 mg/L, about 200 mg/L

to about 375 mg/L, about 200 mg/L to about 400 mg/L, about 250 mg/L to about 275 mg/L, about 250 mg/L

to about 300 mg/L, about 250 mg/L to about 325 mg/L, about 250 mg/L to about 350 mg/L, about 250 mg/L

to about 375 mg/L, about 250 mg/L to about 400 mg/L, about 300 mg/L to about 325 mg/L, about 300 mg/L

to about 350 mg/L, about 300 mg/L to about 375 mg/L, about 300 mg/L to about 400 mg/L, about 350 mg/L

to about 375 mg/L, about 350 mg/L to about 400 mg/L, or about 375 mg/L to about 400 mg/L.

[106] In other aspects of this embodiment, a composition disclosed herein comprises metallic particles

in an amount of, e.g., about 400 mg/L to about 500 mg/L, about 400 mg/L to about 600 mg/L, about 400

mg/L to about 700 mg/L, about 400 mg/L to about 800 mg/L, about 400 mg/L to about 900 mg/L, about 400

mg/L to about 1,000 mg/L, about 400 mg/L to about 1,100 mg/L, about 400 mg/L to about 1,200 mg/L,

about 400 mg/L to about 1,300 mg/L, about 400 mg/L to about 1,400 mg/L, about 400 mg/L to about 1,500

mg/L, about 400 mg/L to about 1,600 mg/L, about 400 mg/L to about 1,700 mg/L, about 400 mg/L to about

1,800 mg/L, about 400 mg/L to about 1,900 mg/L, about 400 mg/L to about 2,000 mg/L, about 500 mg/L to

about 600 mg/L, about 500 mg/L to about 700 mg/L, about 500 mg/L to about 800 mg/L, about 500 mg/L

to about 900 mg/L, about 500 mg/L to about 1,000 mg/L, about 500 mg/L to about 1,100 mg/L, about 500

mg/L to about 1,200 mg/L, about 500 mg/L to about 1,300 mg/L, about 500 mg/L to about 1,400 mg/L,

about 500 mg/L to about 1,500 mg/L, about 500 mg/L to about 1,600 mg/L, about 500 mg/L to about 1,700

mg/L, about 500 mg/L to about 1,800 mg/L, about 500 mg/L to about 1,900 mg/L, about 500 mg/L to about

2,000 mg/L, about 600 mg/L to about 700 mg/L, about 600 mg/L to about 800 mg/L, about 600 mg/L to

about 900 mg/L, about 600 mg/L to about 1,000 mg/L, about 600 mg/L to about 1,100 mg/L, about 600

mg/L to about 1,200 mg/L, about 600 mg/L to about 1,300 mg/L, about 600 mg/L to about 1,400 mg/L,

about 600 mg/L to about 1,500 mg/L, about 600 mg/L to about 1,600 mg/L, about 600 mg/L to about 1,700

mg/L, about 600 mg/L to about 1,800 mg/L, about 600 mg/L to about 1,900 mg/L, about 600 mg/L to about

2,000 mg/L, about 700 mg/L to about 800 mg/L, about 700 mg/L to about 900 mg/L, about 700 mg/L to

about 1,000 mg/L, about 700 mg/L to about 1,100 mg/L, about 700 mg/L to about 1,200 mg/L, about 700

mg/L to about 1,300 mg/L, about 700 mg/L to about 1,400 mg/L, about 700 mg/L to about 1,500 mg/L,

WO wo 2020/252433 PCT/US2020/037665 49

about 700 mg/L to about 1,600 mg/L, about 700 mg/L to about 1,700 mg/L, about 700 mg/L to about 1,800

mg/L, about 700 mg/L to about 1,900 mg/L, about 700 mg/L to about 2,000 mg/L, about 800 mg/L to about

900 mg/L, about 800 mg/L to about 1,000 mg/L, about 800 mg/L to about 1,100 mg/L, about 800 mg/L to

about 1,200 mg/L, about 800 mg/L to about 1,300 mg/L, about 800 mg/L to about 1,400 mg/L, about 800

mg/L to about 1,500 mg/L, about 800 mg/L to about 1,600 mg/L, about 800 mg/L to about 1,700 mg/L,

about 800 mg/L to about 1,800 mg/L, about 800 mg/L to about 1,900 mg/L, about 800 mg/L to about 2,000

mg/L, about 900 mg/L to about 1,000 mg/L, about 900 mg/L to about 1,100 mg/L, about 900 mg/L to about

1,200 mg/L, about 900 mg/L to about 1,300 mg/L, about 900 mg/L to about 1,400 mg/L, about 900 mg/L to

about 1,500 mg/L, about 900 mg/L to about 1,600 mg/L, about 900 mg/L to about 1,700 mg/L, about 900

mg/L to about 1,800 mg/L, about 900 mg/L to about 1,900 mg/L, about 900 mg/L to about 2,000 mg/L,

about 1,000 mg/L to about 1,100 mg/L, about 1,000 mg/L to about 1,200 mg/L, about 1,000 mg/L to about

1,300 mg/L, about 1,000 mg/L to about 1,400 mg/L, about 1,000 mg/L to about 1,500 mg/L, about 1,000

mg/L to about 1,600 mg/L, about 1,000 mg/L to about 1,700 mg/L, about 1,000 mg/L to about 1,800 mg/L,

about 1,000 mg/L to about 1,900 mg/L, about 1,000 mg/L to about 2,000 mg/L, about 1,100 mg/L to about

1,200 mg/L, about 1,100 mg/L to about 1,300 mg/L, about 1,100 mg/L to about 1,400 mg/L, about 1,100

mg/L to about 1,500 mg/L, about 1,100 mg/L to about 1,600 mg/L, about 1,100 mg/L to about 1,700 mg/L,

about 1,100 mg/L to about 1,800 mg/L, about 1,100 mg/L to about 1,900 mg/L, about 1,100 mg/L to about

2,000 mg/L, about 1,200 mg/L to about 1,300 mg/L, about 1,200 mg/L to about 1,400 mg/L, about 1,200

mg/L to about 1,500 mg/L, about 1,200 mg/L to about 1,600 mg/L, about 1,200 mg/L to about 1,700 mg/L,

about 1,200 mg/L to about 1,800 mg/L, about 1,200 mg/L to about 1,900 mg/L, about 1,200 mg/L to about

2,000 mg/L, about 1,300 mg/L to about 1,400 mg/L, about 1,300 mg/L to about 1,500 mg/L, about 1,300

mg/L to about 1,600 mg/L, about 1,300 mg/L to about 1,700 mg/L, about 1,300 mg/L to about 1,800 mg/L,

about 1,300 mg/L to about 1,900 mg/L, about 1,300 mg/L to about 2,000 mg/L, about 1,400 mg/L to about

1,500 mg/L, about 1,400 mg/L to about 1,600 mg/L, about 1,400 mg/L to about 1,700 mg/L, about 1,400

mg/L to about 1,800 mg/L, about 1,400 mg/L to about 1,900 mg/L, about 1,400 mg/L to about 2,000 mg/L,

about 1,500 mg/L to about 1,600 mg/L, about 1,500 mg/L to about 1,700 mg/L, about 1,500 mg/L to about

1,800 mg/L, about 1,500 mg/L to about 1,900 mg/L, about 1,500 mg/L to about 2,000 mg/L, about 1,600

mg/L to about 1,700 mg/L, about 1,600 mg/L to about 1,800 mg/L, about 1,600 mg/L to about 1,900 mg/L,

about 1,600 mg/L to about 2,000 mg/L, about 1,700 mg/L to about 1,800 mg/L, about 1,700 mg/L to about

1,900 mg/L, about 1,700 mg/L to about 2,000 mg/L, about 1,800 mg/L to about 1,900 mg/L, about 1,800

mg/L to about 2,000 mg/L, or about 1,900 mg/L to about 2,000 mg/L.

[107] In aspects of this embodiment, a composition disclosed herein comprises metallic particles in an

metallic particles in an amount of, e.g., at least 0.001%, at least 0.005%, at least 0.01%, at least 0.05%, at

least 0.10%, at least 0.11%, at least 0.12%, at least 0.13%, at least 0.14%, at least 0.15%, at least 0.16%,

at least 0.17%, at least 0.18%, at least 0.19%, at least 0.2%, at least 0.25%, at least 0.3%, at least 0.35%,

at least 0.4%, at least 0.45%, or at least 0.5% by weight of the composition. In yet other aspects of this

WO wo 2020/252433 PCT/US2020/037665 50

embodiment, a composition disclosed herein comprises metallic particles in an amount of, e.g., at most

0.001%, at most 0.005%, at most 0.01%, at most 0.05%, at most 0.10%, at most 0.11%, at most 0.12%, at

most 0.13%, at most 0.14%, at most 0.15%, at most 0.16%, at most 0.17%, at most 0.18%, at most 0.19%,

at most 0.2%, at most 0.25%, at most 0.3%, at most 0.35%, at most 0.4%, at most 0.45%, or at most 0.5%

by weight of the composition. In still other aspects of this embodiment, a composition disclosed herein

comprises metallic particles in an amount of, e.g., about 0.0010% to about 0.005%, about 0.0010% to about

0.010%, about 0.0010% to about 0.050%, about 0.0010% to about 0.10%, about 0.0010% to about 0.15%,

about 0.0010% to about 0.20%, about 0.0010% to about 0.25%, about 0.0010% to about 0.30%, about

0.0010% to about 0.35%, about 0.0010% to about 0.40%, about 0.0010% to about 0.45%, about 0.0010%

to about 0.50%, about 0.010% to about 0.050%, about 0.010% to about 0.10%, about 0.010% to about

0.15%, about 0.010% to about 0.20%, about 0.010% to about 0.25%, about 0.010% to about 0.30%, about

0.010% to about 0.35%, about 0.010% to about 0.40%, about 0.010% to about 0.45%, about 0.010% to

about 0.50%, about 0.10% to about 0.15%, about 0.10% to about 0.20%, about 0.10% to about 0.25%,

about 0.10% to about 0.30%, about 0.10% to about 0.35%, about 0.10% to about 0.40%, about 0.10% to

about 0.45%, about 0.10% to about 0.50%, about 0.20% to about 0.25%, about 0.20% to about 0.30%,

about 0.20% to about 0.35%, about 0.20% to about 0.40%, about 0.20% to about 0.45%, about 0.20% to

about 0.50%, about 0.30% to about 0.35%, about 0.30% to about 0.40%, about 0.30% to about 0.45%,

about 0.30% to about 0.50%, about 0.40% to about 0.45%, about 0.40% to about 0.50%, or about 0.40%

to about 0.50% by weight of the composition.

[108] In an embodiment, metallic particles can be of any size that provides a desired beneficial effect to

a composition disclosed herein. In aspects of this embodiment, metallic particles disclosed herein have a

mean diameter of, e.g., about 10 nm, about 20 nm, about 30 nm, about 40 nm, about 50 nm, about 60 nm,

about 70 nm, about 80 nm, about 90 nm, about 100 nm. In other aspects of this embodiment, metallic

particles disclosed herein have a mean diameter of, e.g., at least 10 nm, at least 20 nm, at least 30 nm, at

least 40 nm, at least 50 nm, at least 60 nm, at least 70 nm, at least 80 nm, at least 90 nm, at least 100 nm.

In yet other aspects of this embodiment, metallic particles disclosed herein have a mean diameter of, e.g.,

at most 10 nm, at most 20 nm, at most 30 nm, at most 40 nm, at most 50 nm, at most 60 nm, at most 70

nm, at most 80 nm, at most 90 nm, at most 100 nm. In yet other aspects of this embodiment, metallic

particles disclosed herein have a mean diameter of, e.g., about 10 nm to about 20 nm, about 10 nm to

about 30 nm, about 10 nm to about 40 nm, about 10 nm to about 50 nm, about 10 nm to about 60 nm,

about 10 nm to about 70 nm, about 10 nm to about 80 nm, about 10 nm to about 90 nm, about 10 nm to

about 100 nm, about 20 nm to about 30 nm, about 20 nm to about 40 nm, about 20 nm to about 50 nm,

about 20 nm to about 60 nm, about 20 nm to about 70 nm, about 20 nm to about 80 nm, about 20 nm to

about 90 nm, about 20 nm to about 100 nm, about 30 nm to about 40 nm, about 30 nm to about 50 nm,

about 30 nm to about 60 nm, about 30 nm to about 70 nm, about 30 nm to about 80 nm, about 30 nm to

about 90 nm, about 30 nm to about 100 nm, about 40 nm to about 50 nm, about 40 nm to about 60 nm,

about 40 nm to about 70 nm, about 40 nm to about 80 nm, about 40 nm to about 90 nm, about 40 nm to

about 100 nm, about 50 nm to about 60 nm, about 50 nm to about 70 nm, about 50 nm to about 80 nm,

about 50 nm to about 90 nm, about 50 nm to about 100 nm, about 60 nm to about 70 nm, about 60 nm to

about 80 nm, about 60 nm to about 90 nm, about 60 nm to about 100 nm, about 70 nm to about 80 nm,

WO wo 2020/252433 PCT/US2020/037665 51 51

about 70 nm to about 90 nm, about 70 nm to about 100 nm, about 80 nm to about 90 nm, about 80 nm to

about 100 nm, or about 90 nm to about 100 nm.

[109] In an embodiment, a composition disclosed herein does not comprise metallic particles.

[110] A composition disclosed herein may comprise a metal salt. A metal salt disclosed herein can be

an alkali metal salt, an alkaline earth metal salt, a base metal salt, or a transition metal salt. An alkali metal

salt includes a lithium salt, a sodium salt, a potassium salt, a rubidium salt, a cesium salt, and a francium

salt. An alkaline earth metal salt includes a beryllium salt, a magnesium salt, a calcium salt, a strontium

salt, a barium salt, and a radium salt. A basic metal salt includes an aluminum salt, a gallium salt, an indium

salt, a tin salt, a thallium salt, a lead salt, and a bismuth salt. A transition metal salt includes a scandium

salt, a titanium salt, a vanadium salt, a chromium salt, a manganese salt, an iron salt, a cobalt salt, a

palladium salt, a silver salt, m a nickel salt, a copper salt, a zinc salt, a yttrium salt, Non-limiting examples

of a metal salt includes a calcium salt, a copper salt, iron salt, a potassium salt, silver salt, titanium salt,

and zinc salt.

[111] Other non-limiting examples of a metal salt includes a metal acetate salt, a metal chloride salt, a

metal nitrate salt, a metal oxide salt, and a metal sulfate salt. A metal acetate salt includes, without

limitation, calcium acetate, copper acetate, iron acetate, e.g., iron (II) acetate and iron (III) acetate,

potassium acetate, silver acetate, titanium acetate, and zinc acetate. A metal chloride salt includes, without

limitation, calcium chloride salt, copper chloride, iron chloride, e.g., iron (II) chloride or iron (III) chloride,

potassium chloride, silver chloride, titanium chloride, and zinc chloride. A metal nitrate salt includes, without

limitation, calcium nitrate salt, copper nitrate, iron nitrate, e.g., iron (II) nitrate, iron (III) nitrate, potassium

nitrate, silver nitrate, titanium nitrate, and zinc nitrate. A metal oxide salt includes, without limitation, calcium

oxide salt, copper oxide salt, iron oxide salt, e.g., iron (II) oxide salt, iron (III) oxide salt, potassium oxide

salt, silver oxide salt, titanium oxide salt, and zinc oxide salt. A metal sulfate salt includes, without limitation,

calcium sulfate, copper sulfate, iron sulfate, e.g., iron (II) sulfate, iron (III) sulfate, potassium sulfate, silver

sulfate, titanium sulfate, and zinc sulfate.

[112] In some embodiments, a composition may include calcium oxide obtained from an organic source

like shells from oceanic organisms. In some embodiment, a composition may include calcium oxide obtain

from an inorganic source like by a synthetic chemical process.

[113] In an embodiment, a composition disclosed herein comprises an amount of a metal salt that

comprising metallic salts is formulated to be used in an undiluted state, the amount of metallic salts used

is a microbiostatic effective amount. A microbiostatic effective amount of metallic salts is an amount that

restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbes. In

an aspect of this embodiment, a microbiostatic effective amount of metallic salts is an amount that restrains,

prohibits, or otherwise prevents microbial population growth without eradicating the microbes to a degree

that achieves disinfection or sterilization. Alternatively, when a composition comprising metallic salts is

WO wo 2020/252433 PCT/US2020/037665 52

formulated to be used in methods where the composition will be diluted, the amount of metallic salts used

in such concentrated formulations can be any amount that does not cause precipitation of components in

the composition disclosed herein or otherwise create unwanted effects that make the composition

inoperable.

[114] In aspects of this embodiment, a composition disclosed herein comprises a metal salt in an amount

of, e.g., 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm, 0.40 ppm, 0.45 ppm,

0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80 ppm, 0.85 ppm, 0.90 ppm, 0.95 ppm,

1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm, 50 ppm, 55 ppm, 60

ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm, 95 ppm, 100 ppm, 125 ppm, 150 ppm, 175 ppm,

200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm, 350 ppm, 375 ppm, 400 ppm, 425 ppm, 450

ppm, 475 ppm, 500 ppm, 525 ppm, 550 ppm, 575 ppm, 600 ppm, 625 ppm, 650 ppm, 675 ppm, 700 ppm,

725 ppm, 750 ppm, 775 ppm, 800 ppm, 825 ppm, 850 ppm, 875 ppm, 900 ppm, 925 ppm, 950 ppm, 975

ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm, 1075 ppm, 1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm, 1,200

ppm, 1,225 ppm, 1,250 ppm, 1,275 ppm, 1,300 ppm, 1,325 ppm, 1,350 ppm, 1,375 ppm, 1,400 ppm, 1,425

ppm, 1,450 ppm, 1,475 ppm, 1,500 ppm, 1,600 ppm, 1,700 ppm, 1,800 ppm, 1,900 ppm, or 2,000 ppm. In

other aspects of this embodiment, a composition disclosed herein comprises a metal salt in an amount of,

e.g., at least 0.05 ppm, at least 0.10 ppm, at least 0.20 ppm, at least 0.30 ppm, at least 0.40 ppm, at least

0.50 ppm, at least 0.60 ppm, at least 0.70 ppm, at least 0.80 ppm, at least 0.90 ppm, at least 1 ppm, at

least 10 ppm, at least 20 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, at least 60 ppm, at least

70 ppm, at least 80 ppm, at least 90 ppm, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least

175 ppm, at least 200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least

325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least

475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least

625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least

775 ppm, at least 800 ppm, at least 825 ppm, at least 850 ppm, at least 875 ppm, at least 900 ppm, at least

925 ppm, at least 950 ppm, at least 975 ppm, at least 1,000 ppm, at least 1,025 ppm, at least 1,050 ppm,

at least 1075 ppm, at least 1,100 ppm, at least 1,125 ppm, at least 1,150 ppm, at least 1,175 ppm, at least

1,200 ppm, at least 1,225 ppm, at least 1,250 ppm, at least 1,275 ppm, at least 1,300 ppm, at least 1,325

ppm, at least 1,350 ppm, at least 1,375 ppm, at least 1,400 ppm, at least 1,425 ppm, at least 1,450 ppm,

at least 1,475 ppm, at least 1,500 ppm, at least 1,600 ppm, at least 1,700 ppm, at least 1,800 ppm, at least

1,900 ppm, or at least 2,000 ppm. In yet other aspects of this embodiment, a composition disclosed herein

comprises a metal salt in an amount of, e.g., at most 0.05 ppm, at most 0.10 ppm, at most 0.20 ppm, at

most 0.30 ppm, at most 0.40 ppm, at most 0.50 ppm, at most 0.60 ppm, at most 0.70 ppm, at most 0.80

ppm, at most 0.90 ppm, at most 1 ppm, at most 10 ppm, at most 20 ppm, at most 30 ppm, at most 40 ppm,

at most 50 ppm, at most 60 ppm, at most 70 ppm, at most 80 ppm, at most 90 ppm, at most 100 ppm, at

most 125 ppm, at most 150 ppm, at most 175 ppm, at most 200 ppm, at most 225 ppm, at most 250 ppm,

at most 275 ppm, at most 300 ppm, at most 325 ppm, at most 350 ppm, at most 375 ppm, at most 400

ppm, at most 425 ppm, at most 450 ppm, at most 475 ppm, at most 500 ppm, at most 525 ppm, at most

550 ppm, at most 575 ppm, at most 600 ppm, at most 625 ppm, at most 650 ppm, at most 675 ppm, at

most 700 ppm, at most 725 ppm, at most 750 ppm, at most 775 ppm, at most 800 ppm, at most 825 ppm, at most 850 ppm, at most 875 ppm, at most 900 ppm, at most 925 ppm, at most 950 ppm, at most 975 ppm, at most 1,000 ppm, at most 1,025 ppm, at most 1,050 ppm, at most 1075 ppm, at most 1,100 ppm, at most 1,125 ppm, at most 1,150 ppm, at most 1,175 ppm, at most 1,200 ppm, at most 1,225 ppm, at most

1,250 ppm, at most 1,275 ppm, at most 1,300 ppm, at most 1,325 ppm, at most 1,350 ppm, at most 1,375

ppm, at most 1,400 ppm, at most 1,425 ppm, at most 1,450 ppm, at most 1,475 ppm, at most 1,500 ppm,

at most 1,600 ppm, at most 1,700 ppm, at most 1,800 ppm, at most 1,900 ppm, or at most 2,000 ppm.

[115] In still other aspects of this embodiment, a composition disclosed herein comprises a metal salt in

an amount of from, e.g., about 0.5 ppm to about 20 ppm, about 0.5 ppm to about 25 ppm, about 0.5 ppm

to about 30 ppm, about 0.5 ppm to about 35 ppm, about 0.5 ppm to about 40 ppm, about 0.5 ppm to about

ppm to about 20 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 30 ppm, about 1 ppm to about

35 ppm, about 1 ppm to about 40 ppm, about 1 ppm to about 45 ppm, about 1 ppm to about 50 ppm, about

1 ppm to about 55 ppm, about 1 ppm to about 60 ppm, about 1 ppm to about 65 ppm, about 1 ppm to about

70 ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 80 ppm, about 1 ppm to about 85 ppm, about

1 ppm to about 90 ppm, about 1 ppm to about 95 ppm, about 1 ppm to about 100 ppm, about 5 ppm to

about 20 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 30 ppm, about 5 ppm to about 35 ppm,

about 5 ppm to about 40 ppm, about 5 ppm to about 45 ppm, about 5 ppm to about 50 ppm, about 5 ppm

to about 55 ppm, about 5 ppm to about 60 ppm, about 5 ppm to about 65 ppm, about 5 ppm to about 70

ppm, about 5 ppm to about 75 ppm, about 5 ppm to about 80 ppm, about 5 ppm to about 85 ppm, about 5

ppm to about 90 ppm, about 5 ppm to about 95 ppm, about 5 ppm to about 100 ppm, about 10 ppm to

about 20 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 30 ppm, about 10 ppm to about 35

ppm, about 10 ppm to about 40 ppm, about 10 ppm to about 45 ppm, about 10 ppm to about 50 ppm, about

10 ppm to about 55 ppm, about 10 ppm to about 60 ppm, about 10 ppm to about 65 ppm, about 10 ppm to

about 70 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 80 ppm, about 10 ppm to about 85

ppm, about 10 ppm to about 90 ppm, about 10 ppm to about 95 ppm, or about 10 ppm to about 100 ppm.

[116] In other aspects of this embodiment, a composition disclosed herein comprises a metal salt in an

amount of from, e.g., about 1 ppm to about 25 ppm, about 1 ppm to about 50 ppm, about 1 ppm to about

75 ppm, about 1 ppm to about 100 ppm, about 1 ppm to about 125 ppm, about 1 ppm to about 150 ppm,

about 1 ppm to about 175 ppm, about 1 ppm to about 200 ppm, about 1 ppm to about 225 ppm, about 1

ppm to about 250 ppm, about 1 ppm to about 275 ppm, about 1 ppm to about 300 ppm, about 1 ppm to

about 325 ppm, about 1 ppm to about 350 ppm, about 1 ppm to about 375 ppm, about 1 ppm to about 400 ppm, about 1 ppm to about 425 ppm, about 1 ppm to about 450 ppm, about 1 ppm to about 475 ppm, about

1 ppm to about 500 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 50 ppm, about 5 ppm to

about 75 ppm, about 5 ppm to about 100 ppm, about 5 ppm to about 125 ppm, about 5 ppm to about 150

ppm, about 5 ppm to about 175 ppm, about 5 ppm to about 200 ppm, about 5 ppm to about 225 ppm, about

5 ppm to about 250 ppm, about 5 ppm to about 275 ppm, about 1 ppm to about 300 ppm, about 5 ppm to

about 325 ppm, about 5 ppm to about 350 ppm, about 5 ppm to about 375 ppm, about 5 ppm to about 400

ppm, about 5 ppm to about 425 ppm, about 5 ppm to about 450 ppm, about 5 ppm to about 475 ppm, about

5 ppm to about 500 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50 ppm, about 10 ppm to

about 75 ppm, about 10 ppm to about 100 ppm, about 10 ppm to about 125 ppm, about 10 ppm to about

150 ppm, about 10 ppm to about 175 ppm, about 10 ppm to about 200 ppm, about 10 ppm to about 225

ppm, about 10 ppm to about 250 ppm, about 10 ppm to about 275 ppm, about 10 ppm to about 300 ppm,

about 10 ppm to about 325 ppm, about 10 ppm to about 350 ppm, about 10 ppm to about 375 ppm, about

10 ppm to about 400 ppm, about 10 ppm to about 425 ppm, about 10 ppm to about 450 ppm, about 10 ppm

to about 475 ppm, about 10 ppm to about 500 ppm, about 25 ppm to about 50 ppm, about 25 ppm to about

to about 400 ppm, about 25 ppm to about 425 ppm, about 25 ppm to about 450 ppm, about 25 ppm to

about 475 ppm, about 25 ppm to about 500 ppm, about 50 ppm to about 75 ppm, about 50 ppm to about

100 ppm, about 50 ppm to about 125 ppm, about 50 ppm to about 150 ppm, about 50 ppm to about 175

ppm, about 50 ppm to about 200 ppm, about 50 ppm to about 225 ppm, about 50 ppm to about 250 ppm,

about 50 ppm to about 275 ppm, about 50 ppm to about 300 ppm, about 50 ppm to about 325 ppm, about

50 ppm to about 350 ppm, about 50 ppm to about 375 ppm, about 50 ppm to about 400 ppm, about 50 ppm

to about 425 ppm, about 50 ppm to about 450 ppm, about 50 ppm to about 475 ppm, about 50 ppm to

about 500 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125 ppm, about 75 ppm to about

150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200 ppm, about 75 ppm to about 225

ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, about 75 ppm to about 300 ppm,

about 75 ppm to about 325 ppm, about 75 ppm to about 350 ppm, about 75 ppm to about 375 ppm, about

75 ppm to about 400 ppm, about 75 ppm to about 425 ppm, about 75 ppm to about 450 ppm, about 75 ppm

to about 475 ppm, about 75 ppm to about 500 ppm, about 100 ppm to about 125 ppm, about 100 ppm to

about 150 ppm, about 100 ppm to about 175 ppm, about 100 ppm to about 200 ppm, about 100 ppm to

about 225 ppm, about 100 ppm to about 250 ppm, about 100 ppm to about 275 ppm, about 100 ppm to

about 300 ppm, about 100 ppm to about 325 ppm, about 100 ppm to about 350 ppm, about 100 ppm to

about 375 ppm, about 100 ppm to about 400 ppm, about 100 ppm to about 425 ppm, about 100 ppm to

about 450 ppm, about 100 ppm to about 475 ppm, about 100 ppm to about 500 ppm, about 150 ppm to

about 175 ppm, about 150 ppm to about 200 ppm, about 150 ppm to about 225 ppm, about 150 ppm to

about 250 ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300 ppm, about 150 ppm to

about 325 ppm, about 150 ppm to about 350 ppm, about 150 ppm to about 375 ppm, about 150 ppm to

about 400 ppm, about 150 ppm to about 425 ppm, about 150 ppm to about 450 ppm, about 150 ppm to

about 475 ppm, about 150 ppm to about 500 ppm, about 200 ppm to about 225 ppm, about 200 ppm to

WO wo 2020/252433 PCT/US2020/037665 55

about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm, about 200 ppm to

about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm, about 200 ppm to

about 400 ppm, about 200 ppm to about 425 ppm, about 200 ppm to about 450 ppm, about 200 ppm to

about 475 ppm, about 200 ppm to about 500 ppm, about 250 ppm to about 275 ppm, about 250 ppm to

about 300 ppm, about 250 ppm to about 325 ppm, about 250 ppm to about 350 ppm, about 250 ppm to

about 375 ppm, about 250 ppm to about 400 ppm, about 250 ppm to about 425 ppm, about 250 ppm to

about 450 ppm, about 250 ppm to about 475 ppm, about 250 ppm to about 500 ppm, about 300 ppm to

about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to

about 400 ppm, about 300 ppm to about 425 ppm, about 300 ppm to about 450 ppm, about 300 ppm to

about 475 ppm, about 300 ppm to about 500 ppm, about 350 ppm to about 375 ppm, about 350 ppm to

about 400 ppm, about 350 ppm to about 425 ppm, about 350 ppm to about 450 ppm, about 350 ppm to

about 475 ppm, about 350 ppm to about 500 ppm, about 400 ppm to about 425 ppm, about 400 ppm to

about 450 ppm, about 400 ppm to about 475 ppm, about 400 ppm to about 500 ppm, about 450 ppm to

about 475 ppm, or about 450 ppm to about 500 ppm.

[117] In other aspects of this embodiment, a composition disclosed herein comprises a metal salt in an

amount of, e.g., about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, about 400 ppm to

about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about 400 ppm to

WO wo 2020/252433 PCT/US2020/037665 56

about 1,900 ppm to about 2,000 ppm.

[118] In aspects of this embodiment, a composition disclosed herein comprises a metal salt in an amount

of, e.g., about 0.001%, about 0.005%, about 0.01%, about 0.05%, about 0.10%, about 0.11%, about 0.12%,

about 0.13%, about 0.14%, about 0.15%, about 0.16%, about 0.17%, about 0.18%, about 0.19%, about

0.2%, about 0.25%, about 0.3%, about 0.35%, about 0.4%, about 0.45%, or about 0.5% by weight of the

composition. In other aspects of this embodiment, a composition disclosed herein comprises a metal salt

in an amount of, e.g., at least 0.001%, at least 0.005%, at least 0.01%, at least 0.05%, at least 0.10%, at

least 0.11%, at least 0.12%, at least 0.13%, at least 0.14%, at least 0.15%, at least 0.16%, at least 0.17%,

at least 0.18%, at least 0.19%, at least 0.2%, at least 0.25%, at least 0.3%, at least 0.35%, at least 0.4%,

at least 0.45%, or at least 0.5% by weight of the composition. In yet other aspects of this embodiment, a

composition disclosed herein comprises a metal salt in an amount of, e.g., at most 0.001%, at most 0.005%,

at most 0.01%, at most 0.05%, at most 0.10%, at most 0.11%, at most 0.12%, at most 0.13%, at most

0.14%, at most 0.15%, at most 0.16%, at most 0.17%, at most 0.18%, at most 0.19%, at most 0.2%, at

most 0.25%, at most 0.3%, at most 0.35%, at most 0.4%, at most 0.45%, or at most 0.5% by weight of the

composition. In still other aspects of this embodiment, a composition disclosed herein comprises a metal

salt in an amount of, e.g., about 0.0010% to about 0.005%, about 0.0010% to about 0.010%, about 0.0010%

to about 0.050%, about 0.0010% to about 0.10%, about 0.0010% to about 0.15%, about 0.0010% to about

WO wo 2020/252433 PCT/US2020/037665 57

0.20%, about 0.0010% to about 0.25%, about 0.0010% to about 0.30%, about 0.0010% to about 0.35%,

about 0.0010% to about 0.40%, about 0.0010% to about 0.45%, about 0.0010% to about 0.50%, about

0.010% to about 0.050%, about 0.010% to about 0.10%, about 0.010% to about 0.15%, about 0.010% to

about 0.20%, about 0.010% to about 0.25%, about 0.010% to about 0.30%, about 0.010% to about 0.35%,

about 0.010% to about 0.40%, about 0.010% to about 0.45%, about 0.010% to about 0.50%, about 0.10%

to about 0.15%, about 0.10% to about 0.20%, about 0.10% to about 0.25%, about 0.10% to about 0.30%,

about 0.35%, about 0.30% to about 0.40%, about 0.30% to about 0.45%, about 0.30% to about 0.50%,

the composition.

[119] In an embodiment, a composition disclosed herein does not comprise metal salts.

[120] A composition disclosed herein may comprise a phosphate buffer. A phosphate buffer or phosphate buffer solution) is especially sensitive to pH changes, and thus is useful for biological

applications. A phosphate buffer can be adapted to a variety of pH levels, including isotonic. This wide

range is due to phosphoric acid having 3 dissociation constants (pKa) allowing for formulation of buffers

near each of the pH levels of 2.15, 7.21, or 12.32. Monosodium phosphate and its conjugate base,

disodium phosphate, are usually used to generate buffers of pH values around 7. Non-limiting examples

of a phosphate buffer include a calcium phosphate buffer and a sodium phosphate buffer.

[121] In an embodiment, a composition disclosed herein comprises an amount of a phosphate buffer that

provides a desired beneficial effect to a composition disclosed herein. In aspects of this embodiment, a

composition disclosed herein comprises a phosphate buffer in an amount of, e.g., 0.05 ppm, 0.10 ppm,

0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm, 0.40 ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm,

0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80 ppm, 0.85 ppm, 0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm,

20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm, 50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm,

80 ppm, 85 ppm, 90 ppm, 95 ppm, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm,

275 ppm, 300 ppm, 325 ppm, 350 ppm, 375 ppm, 400 ppm, 425 ppm, 450 ppm, 475 ppm, 500 ppm, 525

ppm, 550 ppm, 575 ppm, 600 ppm, 625 ppm, 650 ppm, 675 ppm, 700 ppm, 725 ppm, 750 ppm, 775 ppm,

800 ppm, 825 ppm, 850 ppm, 875 ppm, 900 ppm, 925 ppm, 950 ppm, 975 ppm, 1,000 ppm, 1,025 ppm,

1,050 ppm, 1075 ppm, 1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm, 1,200 ppm, 1,225 ppm, 1,250 ppm,

1,275 ppm, 1,300 ppm, 1,325 ppm, 1,350 ppm, 1,375 ppm, 1,400 ppm, 1,425 ppm, 1,450 ppm, 1,475 ppm,

1,500 ppm, 1,600 ppm, 1,700 ppm, 1,800 ppm, 1,900 ppm, or 2,000 ppm. In other aspects of this

embodiment, a composition disclosed herein comprises a phosphate buffer in an amount of, e.g., at least

200 ppm, at least t225ppm,at least250 ppm,at least 275 ppm,at least 300 ppm, at least 325 ppm, at least

350 ppm, at least 375 ppm,atleast400ppm,atleast 425ppm,atleast450 ppm,atleast 475 ppm, at least

650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least

phosphate buffer in an amount of, e.g., at most 0.05 ppm, at most 0.10 ppm, at most 0.20 ppm, at most

0.30 ppm, at most 0.40 ppm, at most 0.50 ppm, at most 0.60 ppm, at most 0.70 ppm, at most 0.80 ppm, at

most 0.90 ppm, at most 1 ppm, at most 10 ppm, at most 20 ppm, at most 30 ppm, at most 40 ppm, at most

50 ppm, at most 60 ppm, at most70ppm,atmost 80 ppm,atmost 90 ppm,atmost 100 ppm, at most 125

ppm, at most 150 ppm, at most 175 ppm, at most 200 ppm, at most 225 ppm, at most 250 ppm, at most

275 ppm, at most 300 ppm, at most 325 ppm, at most 350 ppm, at most 375 ppm, at most 400 ppm, at

most 425 ppm, at most 450 ppm, at most 475 ppm, at most 500 ppm, at most 525 ppm, at most 550 ppm,

at most 575 ppm, at most 600 ppm, at most 625 ppm, at most 650 ppm, at most 675 ppm, at most 700

ppm, at most 725 ppm, at most 750 ppm, at most 775 ppm, at most 800 ppm, at most 825 ppm, at most

850 ppm, at most 875 ppm, at most 900 ppm, at most 925 ppm, at most 950 ppm, at most 975 ppm, at

most 1,000 ppm, at most 1,025 ppm, at most 1,050 ppm, at most 1075 ppm, at most 1,100 ppm, at most

1,125 ppm, at most 1,150 ppm, at most 1,175 ppm, at most 1,200 ppm, at most 1,225 ppm, at most 1,250

ppm, at most 1,275 ppm, at most 1,300 ppm, at most 1,325 ppm, at most 1,350 ppm, at most 1,375 ppm,

at most 1,400 ppm, at most 1,425 ppm, at most 1,450 ppm, at most 1,475 ppm, at most 1,500 ppm, at most

1,600 ppm, at most 1,700 ppm, at most 1,800 ppm, at most 1,900 ppm, or at most 2,000 ppm.

[122] In still other aspects of this embodiment, a composition disclosed herein comprises a phosphate

buffer in an amount of from, e.g., about 0.5 ppm to about 20 ppm, about 0.5 ppm to about 25 ppm, about

70 ppm, about 0.75 ppm to about 75 ppm, about 0.75 ppm to about 80 ppm, about 0.75 ppm to about 85

ppm, about 0.75 ppm to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75 ppm to about 100

ppm, about 1 ppm to about 20 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 30 ppm, about 1

ppm to about 35 ppm, about 1 ppm to about 40 ppm, about 1 ppm to about 45 ppm, about 1 ppm to about

WO wo 2020/252433 PCT/US2020/037665 59

to about 100 ppm.

[123] In other aspects of this embodiment, a composition disclosed herein comprises a phosphate buffer

ppm, about 1 ppm to about 425 ppm, about 1 ppm to about 450 ppm, about 1 ppm to about 475 ppm, about

about 475 ppm, or about 450 ppm to about 500 ppm.

[124] In an embodiment, a composition disclosed herein does not comprise a phosphate buffer. In an

embodiment, a composition disclosed herein does not comprise a calcium phosphate buffer. In an

embodiment, a composition disclosed herein does not comprise a sodium phosphate buffer.

[125] A composition disclosed herein may comprise a surfactant. Surfactants are compounds that lower

the surface tension of a liquid, allowing easier spreading, and lowering of the interfacial tension between

two liquids, or between a liquid and a solid. By acting at the interface of two surfaces, surfactants modulate

the interfacial tension resulting in the formation of the foam. Either a single surfactant may be mixed with

the buffered solution disclosed herein, or a plurality of surfactants may be mixed with the buffered solution

disclosed herein. Useful surfactants, include, without limitation, ionic surfactants, zwitterionic (amphoteric)

surfactants, non-ionic surfactants, or any combination therein. The surfactant used in a composition

disclosed herein can be varied as appropriate by one skilled in the art and generally depends, in part, on

the particular buffer being used, the protein being eluted, and the conductivity values being employed.

[126] Ionic surfactants include anionic surfactants. Anionic surfactants include ones based on permanent

functional groups attached to the head, such as, e.g., sulfate, sulfonate, phosphate carboxylates) or pH

dependent anionic surfactants. Anionic surfactants include, without limitation, alkyl sulfates like ammonium

lauryl sulfate and sodium lauryl sulfate (SDS); alkyl ether sulfates like sodium laureth sulfate and sodium

myreth sulfate; docusates like dioctyl sodium sulfosuccinate; sulfonate fluorosurfactants like

perfluorooctanesulfonate (PFOS) and perfluorobutanesulfonate; alkyldiphenyloxide Disulfonates like

DOWFAX 2A1 (Disodium Lauryl Phenyl Ether Disulfonate), DOWFAX 3B2 (Disodium Decyl Phenyl Ether Disulfonate), DOWFAX C10L (Disodium Decyl Phenyl Ether Disulfonate), DOWFAX 2EP, and DOWFAX 8390 (Disodium Cetyl Phenyl Ether Disulfonate); potassium phosphate polyether esters like

TRITON H-55 and TRITON H-66; alkyl benzene sulfonates; alkyl aryl ether phosphates; alkyl ether

phosphates; alkyl carboxylates like fatty acid salts and sodium stearate; sodium lauroyl sarcosinate;

carboxylate fluorosurfactants like perfluorononanoate and perfluorooctanoate; and Sodium Hexyldiphenyl

Ether Sulfonate (DOWFAX C6L).

[127] Ionic surfactants also include cationic surfactants. Cationic surfactants include ones based on

permanent or pH dependent cationic surfactants, such as, e.g., primary, secondary or tertiary amines.

Cationic surfactants include, without limitation, quaternary ammonium surfactants like alkyl-

trimethylammonium salts and alkyl-benzyldimethylammonium salts as well as pH-dependent primary,

secondary or tertiary amines like surfactants where the primary amines become positively charged at pH

greater than 10, or the secondary amines become charged at pH less than 4, like octenidine dihydrochloride. Non-limiting examples of quaternary ammonium surfactants include first generation

quaternary ammonium surfactants comprising C12-18 alkyl chains, second generation quaternary ammonium

surfactants comprising aromatic ring groups, third generation quaternary ammonium surfactants

comprising dual first generation quaternary ammonium compounds and/or mixtures of alkyl dimethyl

ammonium chloride, fourth generation quaternary ammonium surfactants comprising dialkylmethyl aminos

with twin chains, fifth generation quaternary ammonium surfactants comprising synergistic combinations of

dual quaternary ammonium compounds, six generation quaternary ammonium surfactants comprising

polymeric quaternary ammonium compounds, and seventh generation quaternary ammonium surfactants

comprising bis-quaternary ammonium compounds with polymeric quaternary ammonium compounds. Quaternary ammonium surfactants include, without limitation, benzalkonium chloride (BAC), benzethonium

chloride (BZT), benzododecinium bromide (or dimethyldodecylbenzylammonium bromide), bronidox, (or 5- bromo-5-nitro-1,3-dioxane), carbethopendecinium bromide, cetalkonium chloride (CKC), cetrimonium, cetrimide, cetylpyridinium chloride (CPC), cetylpyridinium bromide, cetyl trimethylammonium bromide

(CTAB or cetrimonium bromide) and cetyl trimethylammonium chloride (CTAC or cetrimonium chloride),

didecyldimethylammonium chloride (DDAC), dioctadecyldimethylammonium bromide (DODAB or

dimethyldioctadecylammonium bromide), dioctadecyldimethylammonium chloride (DODAC or dimethyldioctadecylammonium chloride), docosyltrimethylammonium chloride (DCTAC or behentrimonium

chloride), dofanium chloride, domiphen bromide, methylbenzethonium chloride, octenidine dihydrochloride,

polidronium chloride, stearalkonium chloride (or dimethylbenzyloctadecylammonium), tetraethylammonium

bromide, tetramethylammonium hydroxide (TMAH) and thonzonium bromide. Other useful anionic surfactants include bio-based anionic surfactants, including, without limitation, STEPONOL® AM 30-KE, an

ammonium lauryl sulfate, and STEPONOL® EHS, a sodium 2-ethyl hexyl sulfate. Such bio-based surfactants are not synthetic molecules, but instead are anionic biosurfactants derived from organic matter

such as plants.

[128] Zwitterionic surfactants are based on primary, secondary or tertiary amines or quaternary

ammonium cation with a sulfonate, a carboxylate, or a phosphate. Zwitterionic surfactants include, without

limitation, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); sultaines like

cocamidopropyl hydroxysultaine; betaines like cocamidopropyl betaine; or lecithins.

[129] Non-ionic surfactants are less denaturing and as such are useful to solubilize membrane proteins

and lipids while retaining protein-protein interactions. Nonionic surfactants include polyether nonionic

surfactants, polyhydroxyl nonionic surfactants and biosurfactants. Nonionic surfactants include alcohol

ethoxylates, alkylphenol ethoxylates, phenol ethoxylates, amide ethoxylates, glyceride ethoxylates, fatty

acid ethoxylates, fatty amine ethoxylates and polyethoxylated tallow amine (POEA). Other useful non-ionic

surfactants include bio-based non-ionic surfactants, including, without limitation, STEPOSOL® MET-10U, a

metathesis-derived, nonionic surfactant that is an unsaturated, short chain amide. Such bio-based

surfactants are not synthetic molecules, but instead are non-ionic biosurfactants derived from organic

matter such as plants.

[130] Non-limiting examples of surfactants include polyoxyethylene glycol sorbitan alkyl esters (or

ethoxylated sorbital esters) like polysorbate 20 sorbitan monooleate (TWEEN 20), polysorbate 40 sorbitan

monooleate (TWEEN 40), polysorbate 60 sorbitan monooleate (TWEEN 60), polysorbate 61 sorbitan

monooleate (TWEEN 61), polysorbate 65 sorbitan monooleate (TWEEN 65), polysorbate 80 sorbitan

monooleate (TWEEN 80), polysorbate 81 sorbitan monooleate (TWEEN 81) and polysorbate 85 sorbitan

monooleate (TWEEN® 85); sorbital esters like sorbitan monooleate, sorbitan monolaurate, sorbitan

monopalmitate, sorbitan monostearate and sorbitan tristearate; polyglycerol esters like glycerol

monooleate, glycerol monolaurate, glycerol monopalmitate, glycerol monostearate, glycerol trioleate,

glycerol ricinoleate, glycerol tristearate, mono diglycerides and glycerol triacetate; ethoxylated polyglycerol

esters; alkyl glucosides like arachidyl glucoside, C12-20 alkyl glucoside, caprylyl/capryl glucoside, cetearyl

glucoside, coco-glucoside, ethyl glucoside and lauryl glucoside. decyl glucoside; ethoxylated alkyl

glucosides; sucrose esters like sucrose monooleate, sucrose monolaurate, sucrose monopalmitate, sucrose monostearate, sucrose trioleate, sucrose ricinoleate, sucrose tristearate, sucrose diglycerides and sucrose triacetate; ethoxylated sucrose ester; amine oxides; ethoxylated alcohols; ethoxylated aliphatic alcohols; alkylamines; ethoxylated alkylamines; ethoxylated alkyl phenols like ethoxylated nonyl phenol and ethoxylated octyl phenol; alkyl polysaccharides; ethoxylated alkyl polysaccharides; ethoxylated fatty acids like ethoxylated castor oil; ethoxylated fatty alcohols like ethoxylated ceto-oleyl alcohol, ethoxylated ceto- stearyl alcohol, ethoxylated decyl alcohol, ethoxylated dodecy alcohol and ethoxylated tridecyl alcohol; ethoxylated fatty amines; poloxamers (polyethylene-polypropylene copolymers), like Poloxamer 124

(PLURONIC® L44), Poloxamen 181 (PLURONIC L61), Poloxamer 182 (PLURONIC L62), Poloxamer 184

(PLURONIC® L64), Poloxamen 188 (PLURONIC F68), Poloxamen 237 (PLURONIC® F87), Poloxamen 338 (PLURONIC® L108), and Poloxamer 407 (PLURONIC® F127); linear secondary alcohol ethoxylates like

TERGITOLTM 15-S-5, TERGITOLTM 15-S-7, TERGITOLTM 15-S-9, TERGITOLTM 15-S-12, TERGITOLTM 15-S-15, TERGITOL 15-S-20, TERGITOLTM 15-S-30 and TERGITOLTM 15-S-40; alkyl phenol polyglycol

ethers; polyethylene glycol alkyl aryl ethers; polyoxyethylene glycol alkyl ethers, like octaethylene glycol

monododecyl ether, pentaethylene glycol monododecyl ether, BRIJ® 30, and BRIJ® 35; 2-dodecoxyethanol

(LUBROL ); polyoxyethylene glycol octylphenol ethers like polyoxyethylene (4-5) p-t-octyl phenol

(TRITON X-45) and polyoxyethylene octyl phenyl ether (TRITON X-100); polyoxyethylene glycol alkylphenol ethers like Nonoxynol-9; phenoxypolyethoxylethanols like nonylphenoxypolyethoxylethanol

and octylphenoxypolyethoxylethanol (IGEPAL® CA-630 or NONIDET P-40); glucoside alkyl ethers like

octyl glucopyranoside; maltoside alkyl ethers like dodecyl maltopyranoside; thioglucoside alkyl ethers like

heptyl thioglucopyranoside; digitonins; glycerol alkyl esters like glyceryl laurate; alkyl aryl polyether sulfates;

alcohol sulfonates; sorbitan alkyl esters; cocamide ethanolamines like cocamide monoethanolamine and

cocamide diethanolamine; sucrose monolaurate; dodecy dimethylamine oxide, and sodium cholate. Other

non-limiting examples of surfactants useful in the methods disclosed herein can be found in, e.g., Winslow,

et al., Methods and Compositions for Simultaneously Isolating Hemoglobin from Red Blood Cells and

Inactivating Viruses, U.S. 2008/0138790; Pharmaceutical Dosage Forms and Drug Delivery Systems

(Howard C. Ansel et al., eds., Lippincott Williams & Wilkins Publishers, 7th ed. 1999); Remington: The

Science and Practice of Pharmacy (Alfonso R. Gennaro ed., Lippincott, Williams & Wilkins, 20th ed. 2000);

Goodman & Gilman's The Pharmacological Basis of Therapeutics (Joel G. Hardman et al., eds., McGraw-

Hill Professional, 10th ed. 2001); and Handbook of Pharmaceutical Excipients (Raymond C. Rowe et al.,

APhA Publications, 4th edition 2003), each of which is hereby incorporated by reference in its entirety.

[131] In one embodiment, a single surfactant is present in a composition disclosed herein. In another

embodiment, a plurality of surfactants is present in a composition disclosed herein. In aspects of this

embodiment, a composition disclosed herein comprises, e.g., one or more surfactants, two or more

surfactants, three or more surfactants, four or more surfactants or five or more surfactants. In other aspects

of this embodiment, a composition disclosed herein comprises, e.g., only one surfactant, at most two

surfactants, at most three surfactants, at most four surfactants, or at most five surfactants. In yet other

aspects of this embodiment, a composition disclosed herein comprises from, e.g., 1 to 2 surfactants, 1 to 3

surfactants, 1 to 4 surfactants, 1 to 5 surfactants, 2 to 3 surfactants, 2 to 4 surfactants, 2 to 5 surfactants,

3 to 4 surfactants, 3 to 5 surfactants or 4 to 5 surfactants.

[132] Any amount of a surfactant disclosed herein may be used, with the proviso that the amount is useful

to practice the methods disclosed herein. For example, when a composition comprising a surfactant is

formulated to be used in an undiluted state, the amount of a surfactant used is a microbiostatic effective

amount. A microbiostatic effective amount of a surfactant is an amount that restrains, prohibits, or otherwise

microbiostatic effective amount of a surfactant is an amount that restrains, prohibits, or otherwise prevents

sterilization. Alternatively, when a composition comprising a surfactant is formulated to be used in methods

where the composition will be diluted, the amount of a surfactant used in such concentrated formulations

otherwise create unwanted effects that make the composition inoperable.

[133] In aspects of this embodiment, a composition disclosed herein comprises a surfactant in an amount

of, e.g., about 0.001%, about 0.005%, about 0.0075%, about 0.01%, about 0.05%, about 0.075%, about

0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%,

about 1.0%, about 1.5%, about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about 6.0%, about

7.0%, about 7.5%, about 8.0%, about 9.0% or about 10.0% by weight of the composition. In other aspects

of this embodiment, a composition disclosed herein comprises a surfactant in an amount of, e.g., at least

0.001%, at least 0.005%, at least 0.0075%, at least 0.01%, at least 0.05%, at least 0.075%, at least 0.1%,

at least 0.25%, at least 0.5%, at least 0.75%, at least 1.0%, at least 1.5%, at least 2.0%, at least 2.5%, at

least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at least 7.0%, at least 7.5%, at least 8.0%, at least

9.0%, or at least 10.0% by weight of the composition. In yet other aspects of this embodiment, a

composition disclosed herein comprises a surfactant in an amount of, e.g., at most 0.001%, at most 0.005%,

at most 0.0075%, at most 0.01%, at most 0.05%, at most 0.075%, at most 0.1%, at most 0.25%, at most

4.0%, at most 5.0%, at most 6.0%, at most 7.5%, at most 8.0%, at most 9.0% or at most 10.0% by weight

of the composition.

[134] In still other aspects of this embodiment, a composition disclosed herein comprises a surfactant in

an amount of, e.g., about 0.001% to about 0.005%, about 0.001% to about 0.0075%, about 0.001% to

about 0.01%, about 0.001% to about 0.025%, about 0.001% to about 0.05%, about 0.001% to about

0.075%, about 0.001% to about 0.1%, about 0.001% to about 0.25%, about 0.001% to about 0.75%, about

0.001% to about 1.0%, about 0.005% to about 0.0075%, about 0.005% to about 0.01%, about 0.005% to

about 0.025%, about 0.005% to about 0.05%, about 0.005% to about 0.075%, about 0.005% to about 0.1%,

about 0.005% to about 0.25%, about 0.005% to about 0.75%, about 0.005% to about 1.0%, about 0.01%

to about 0.05%, about 0.01% to about 0.075%, about 0.01% to about 0.1%, about 0.01% to about 0.25%,

about 0.01% to about 0.5%, about 0.01% to about 0.75%, about 0.01% to about 1.0%, about 0.01% to

about 1.5%, about 0.01% to about 2.0%, about 0.01% to about 2.5%, about 0.05% to about 0.075%, about

0.05% to about 0.1%, about 0.05% to about 0.25%, about 0.05% to about 0.5%, about 0.05% to about

0.75%, about 0.05% to about 1.0%, about 0.05% to about 1.5%, about 0.05% to about 2.0%, about 0.05%

to about 2.5%, about 0.1% to about 0.5%, about 0.1% to about 0.75%, about 0.1% to about 1.0%, about

WO wo 2020/252433 PCT/US2020/037665 65

0.1% to about 1.5%, about 0.1% to about 2.0%, about 0.1% to about 2.5%, about 0.1% to about 3.0%,

about 0.1% to about 4.0%, about 0.1% to about 5.0%, about 0.2% to about 0.5%, about 0.2% to about

0.75%, about 0.2% to about 1.0%, about 0.2% to about 1.5%, about 0.2% to about 2.0%, about 0.2% to

about 2.5%, about 0.2% to about 3.0%, about 0.2% to about 4.0%, about 0.2% to about 5.0%, about 0.5%

to about 1.0%, about 0.5% to about 1.5%, about 0.5% to about 2.0%, about 0.5% to about 2.5%, about

0.5% to about 3.0%, about 0.5% to about 4.0%, about 0.5% to about 5.0%, about 0.5% to about 6.0%,

about 0.5% to about 7.0%, about 0.5% to about 8.0%, about 0.5% to about 9.0%, about 0.5% to about

10.0%, about 1.0% to about 2.5%, about 1.0% to about 3.0%, about 1.0% to about 4.0%, about 1.0% to

about 5.0%, about 1.0% to about 6.0%, about 1.0% to about 7.0%, about 1.0% to about 7.5%, about 1.0%

to about 8.0%, about 1.0% to about 9.0%, about 1.0% to about 10.0%, about 2.0% to about 2.5%, about

2.0% to about 3.0%, about 2.0% to about 4.0%, about 2.0% to about 5.0%, about 2.0% to about 6.0%,

about 2.0% to about 7.0%, about 2.0% to about 7.5%, about 2.0% to about 8.0%, about 2.0% to about

9.0%, about 2.0% to about 10.0%, about 5.0% to about 6.0%, about 5.0% to about 7.0%, about 5.0% to

about 7.5%, about 5.0% to about 8.0%, about 5.0% to about 9.0%, about 5.0% to about 10.0%, about 5.0%

to about 11.0%, about 5.0% to about 12.0%, about 5.0% to about 13.0%, about 5.0% to about 14.0%, about

5.0% to about 15.0%, about 6.0% to about 7.0%, about 6.0% to about 7.5%, about 6.0% to about 8.0%,

about 6.0% to about 9.0% about 6.0% to about 10.0%, about 6.0% to about 11.0%, about 6.0% to about

12.0%, about 6.0% to about 13.0%, about 6.0% to about 14.0% or about 6.0% to about 15.0% by weight

of the composition.

[135] In another embodiment, a composition disclosed herein does not comprise a surfactant.

[136] A composition disclosed herein may comprise a carrier. A carrier can be any material typically

known in the skin care, cosmetic and medical arts that is used as a base to formulate a composition

disclosed herein. A carrier may be an aqueous carrier, a semi-solid carrier or a solid carrier. A carrier can

also provide a skin care benefit as disclosed herein. A carrier has substantially no long term or permanent

detrimental effect when administered and encompasses terms such as pharmacologically acceptable

vehicle, stabilizer, diluent, additive, auxiliary, or excipient. Such a carrier generally is mixed with one or

more active compounds or permitted to dilute or enclose one or more active compounds and can be a solid,

semi-solid, or liquid. It is understood that the one or more active ingredients can be soluble or can be

delivered as a suspension in the desired carrier or diluent. A carrier includes, without limitation, water, a

vegetable oil, a mineral oil, an ester oil, an ether, an alcohol, a fatty alcohol, an isoparaffin, a hydrocarbon

oil, a polyol, and a wax. Non-limiting examples of an ester oil include octal palmitate, isopropyl myristate

and isopropyl palmitate. Non-limiting examples of an ether includes dicapryl ether and dimethyl isosorbide.

Non-limiting examples of an alcohol includes ethanol and isopropanol. Non-limiting examples of a fatty

alcohol include cetyl alcohol, cetearyl alcohol, stearyl alcohol and behenyl alcohol. Non-limiting examples

of an isoparaffin include isooctane, isododecane (IDD) and isohexadecane. Non-limiting examples of a hydrocarbon oil include mineral oil, petrolatum, isoeicosane and a polyolefin, including (hydrogenated)

polyisobutene. Non-limiting examples of a polyol include propylene glycol, glycerin, butylene glycol,

pentylene glycol, hexylene glycol, caprylyl glycol. Non-limiting examples of a wax include beeswax,

carnauba, ozokerite, microcrystalline wax, polyethylene wax, and a botanical wax.

PCT/US2020/037665 66

[137] In one embodiment, a single carrier is present in a composition disclosed herein. In another

embodiment, a plurality of carriers is present in a composition disclosed herein. In aspects of this

embodiment, a composition disclosed herein comprises, e.g., one or more carriers, two or more carriers,

three or more carriers, four or more carriers or five or more carriers. In other aspects of this embodiment,

a composition disclosed herein comprises, e.g., only one carrier, at most two carriers, at most three carriers,

at most four carriers, or at most five carriers. In yet other aspects of this embodiment, a composition

disclosed herein comprises from, e.g., 1 to 2 carriers, 1 to 3 carriers, 1 to 4 carriers, 1 to 5 carriers, 2 to 3

carriers, 2 to 4 carriers, 2 to 5 carriers, 3 to 4 carriers, 3 to 5 carriers or 4 to 5 carriers.

[138] In another embodiment, a composition disclosed herein comprises an amount of carrier that

provides a desired formulative or beneficial effect to a composition disclosed herein. In aspects of this

embodiment, a composition disclosed herein comprises a carrier in an amount of, e.g., at least 5%, at least

10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at

least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%,

at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% by weight of the

composition. In other aspects of this embodiment, a composition disclosed herein comprises a carrier in

an amount of, e.g., at most 5%, at most 10%, at most 15%, at most 20%, at most 25%, at most 30%, at

most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%,

at most 75%, at most 80%, at most 85%, at most 90%, at most 95%, at most 96%, at most 97%, at most

98% or at most 99% by weight of the composition. In yet other aspects of this embodiment, a composition

disclosed herein comprises a carrier in an amount of from, e.g., about 5% to about 25%, about 5% to about

50%, about 5% to about 75%, about 5% to about 90%, about 5% to about 95%, about 5% to about 96%,

about 5% to about 97%, about 5% to about 98%, about 5% to about 99%, about 25% to about 50%, about

25% to about 75%, about 25% to about 90%, about 25% to about 95%, about 25% to about 96%, about

25% to about 97%, about 25% to about 98%, about 25% to about 99%, about 50% to about 75%, about

50% to about 90%, about 50% to about 95%, about 50% to about 96%, about 50% to about 97%, about

50% to about 98%, about 50% to about 99%, about 75% to about 80%, about 75% to about 85%, about

75% to about 90%, about 75% to about 95%, about 75% to about 96%, about 75% to about 97%, about

75% to about 98%, about 75% to about 99%, about 80% to about 85%, about 80% to about 90%, about

80% to about 95%, about 80% to about 96%, about 80% to about 97%, about 80% to about 98%, about

80% to about 99%, about 85% to about 90%, about 85% to about 95%, about 85% to about 96%, about

85% to about 97%, about 85% to about 98%, about 85% to about 99%, about 90% to about 95%, about

90% to about 96%, about 90% to about 97%, about 90% to about 98%, about 90% to about 99%, or about

95% to about 99%, by weight of the composition.

[139] In another embodiment, a composition disclosed herein comprises a carrier which is not an alcohol.

In aspects of this embodiment, when a composition disclosed herein comprises hypochlorous acid or free

available chlorine, then the composition may comprise a carrier where the carrier is not an alcohol.

[140] A composition disclosed herein may further optionally include additional ingredients. An additional

ingredient is one known to be useful in finishing a composition disclosed herein. An additional ingredient

includes, without limitation, a buffer, a hydrophilic clay, a disinfectant, an antiseptic, a preservative, or a

chelating agent. An additional ingredient disclosed herein is known in the art.

[141] Various buffers and means for adjusting pH can be used to prepare a microbiostatic composition

disclosed herein, provided that the resulting preparation is pharmacologically acceptable. Such buffers

include, without limitation, acetate buffers, citrate buffers, phosphate buffers, neutral buffered saline,

phosphate buffered saline and borate buffers. It is understood that acids or bases can be used to adjust

the pH of a composition as needed. Additional acceptable antioxidants include, without limitation, sodium

metabisulfite, sodium thiosulfate, acetylcysteine, butylated hydroxyanisole and butylated hydroxytoluene.

[142] A hydrophilic clay is a synthetic or naturally occurring smectic clay that forms three-dimensional

colloidal structures when hydrated leading to increased viscosity and improved suspension control

components in a composition. A hydrophilic clay includes hydrous phyllosilicate clays. A hydrophilic clay

can have thixotrophic properties. Non-limiting examples of a hydrophilic clay include a bentonite clay, a

hectorite clay, a kaolinite clay, and a silicate clay. A bentonite clay is a phyllosilicate clay and includes,

without limitation, a potassium bentonite clay, a sodium bentonite clay, a calcium bentonite clay, and an

aluminum bentonite clay. A hectorite clay is a phyllosilicate clay and includes those commercially sold as

the BENTONE® product line, including hectorite clay (BENTONE EW), hectorite clay (BENTONE MA),

and hectorite clay and hydroxy ethyl cellulose (BENTONE LT). A kaolinite clay is a phyllosilicate clay. A

silicate clay is a synthetic layered silicate clay and includes those commercially sold as the LAPONITE®

product line, including lithium magnesium sodium silicate (LAPONITE XLG), lithium magnesium sodium

silicate and tetrasodium pyrophosphate (LAPONITE XLS), sodium magnesium fluorosilicate (LAPONITE

XL), and sodium magnesium fluorosilicate (LAPONITE XL21).

[143] In one embodiment, a single hydrophilic clay is present in a composition disclosed herein. In

another embodiment, a plurality of hydrophilic clays is present in a composition disclosed herein. In aspects

of this embodiment, a composition disclosed herein comprises, e.g., one or more hydrophilic clays, two or

more hydrophilic clays, three or more hydrophilic clays, four or more hydrophilic clays or five or more

hydrophilic clays. In other aspects of this embodiment, a composition disclosed herein comprises, e.g.,

only one hydrophilic clay, at most two hydrophilic clays, at most three hydrophilic clays, at most four

hydrophilic clays, or at most five hydrophilic clays. In yet other aspects of this embodiment, a composition

disclosed herein comprises from, e.g., 1 to 2 hydrophilic clays, 1 to 3 hydrophilic clays, 1 to 4 hydrophilic

clays, 1 to 5 hydrophilic clays, 2 to 3 hydrophilic clays, 2 to 4 hydrophilic clays, 2 to 5 hydrophilic clays, 3

to 4 hydrophilic clays, 3 to 5 hydrophilic clays or 4 to 5 hydrophilic clays.

[144] In another embodiment, a composition disclosed herein comprises an amount of a hydrophilic clay

that provides a desired beneficial effect to a composition disclosed herein. In aspects of this embodiment,

a composition disclosed herein comprises a hydrophilic clay in an amount of, e.g., 0.01%, 0.025%, 0.05%,

0.075%, 0.1%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.75%, 0.8%, 0.9%, 1%, 2%, 3%, 4%, 5%,

WO wo 2020/252433 PCT/US2020/037665 68

6%, 7%, 8%. 9%, or 10% by weight of the composition. In other aspects of this embodiment, a composition

disclosed herein comprises a hydrophilic clay in an amount of, e.g., at least 0.01%, at least 0.025%, at least

0.05%, at least 0.075%, at least 0.1%, at least 0.25%, at least 0.5%, at least 0.75%, at least 1%, at least

2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9%, or at least 10%

by weight of the composition. In yet other aspects of this embodiment, a composition disclosed herein

comprises a hydrophilic clay in an amount of, e.g., at most 0.01%, at most 0.025%, at most 0.05%, at most

0.075%, at most 0.1%, at most 0.25%, at most 0.5%, at most 0.75%, at most 1%, at most 2%, at most 3%,

at most 4%, at most 5%, at most 6%, at most 7%, at most 8%, at most 9%, or at most 10% by weight of

the composition.

[145] In still other aspects of this embodiment, a composition disclosed herein comprises a hydrophilic

clay in an amount of from, e.g., about 0.01% to about 0.05%, 0.01% to about 0.075%, about 0.01% to about

0.1%, about 0.1% to about 0.5%, about 0.1% to about 0.75%, about 0.1% to about 1%, about 0.1% to about

2%, about 0.1% to about 3%, about 0.1% to about 4%, about 0.1% to about 5%, about 0.1% to about 6%,

about 0.1% to about 7%, about 0.1% to about 8%, about 0.1% to about 9%, about 0.1% to about 10%,

about 0.25% to about 0.5%, about 0.25% to about 0.75%, about 0.25% to about 1%, about 0.25% to about

2%, about 0.25% to about 3%, about 0.25% to about 4%, about 0.25% to about 5%, about 0.25% to about

6%, about 0.25% to about 7%, about 0.25% to about 8%, about 0.25% to about 9%, about 0.25% to about

10%, about 0.5% to about 0.75%, about 0.5% to about 1%, about 0.5% to about 2%, about 0.5% to about

3%, about 0.5% to about 4%, about 0.5% to about 5%, about 0.5% to about 6%, about 0.5% to about 7%,

about 0.5% to about 8%, about 0.5% to about 9%, about 0.5% to about 10%, about 0.75% to about 1%,

about 0.75% to about 2%, about 0.75% to about 3%, about 0.75% to about 4%, about 0.75% to about 5%,

about 0.75% to about 6%, about 0.75% to about 7%, about 0.75% to about 8%, about 0.75% to about 9%,

about 0.75% to about 10%, about 1% to about 2%, about 1% to about 3%, about 1% to about 4%, about

1% to about 5%, about 1% to about 6%, about 1% to about 7%, about 1% to about 8%, about 1% to about

9%, about 1% to about 10%, about 2% to about 3%, about 2% to about 4%, about 2% to about 5%, about

2% to about 6%, about 2% to about 7%, about 2% to about 8%, about 2% to about 9%, about 2% to about

10%, about 3% to about 4%, about 3% to about 5%, about 3% to about 6%, about 3% to about 7%, about

3% to about 8%, about 3% to about 9%, about 3% to about 10%, about 4% to about 5%, about 4% to about

6%, about 4% to about 7%, about 4% to about 8%, about 4% to about 9%, about 4% to about 10%, about

5% to about 6%, about 5% to about 7%, about 5% to about 8%, about 5% to about 9%, about 5% to about

10%, about 6% to about 7%, about 6% to about 8%, about 6% to about 9%, about 6% to about 10%, about

7% to about 8%, about 7% to about 9%, about 7% to about 10%, about 8% to about 9%, about 8% to about

10% or about 9% to about 10% by weight of the composition.

[146] In another embodiment, a composition disclosed herein comprises an amount of a silicate clay that

composition disclosed herein comprises a silicate clay in an amount of, e.g., 0.001%, 0.0025%, 0.005%,

0.0075%, 0.01%, 0.025%, 0.05%, 0.075%, 0.1%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.75%,

0.8%, 0.9%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%. 9%, or 10% by weight of the composition. In other aspects

of this embodiment, a composition disclosed herein comprises a silicate clay in an amount of, e.g., at least

0.001%, at least 0.0025%, at least 0.005%, at least 0.0075%, at least 0.01%, at least 0.025%, at least

comprises a silicate clay in an amount of, e.g., at most 0.001%, at most 0.0025%, at most 0.005%, at most

0.0075%, at most 0.01%, at most 0.025%, at most 0.05%, at most 0.075%, at most 0.1%, at most 0.25%,

at most 0.5%, at most 0.75%, at most 1%, at most 2%, at most 3%, at most 4%, at most 5%, at most 6%,

at most 7%, at most 8%, at most 9%, or at most 10% by weight of the composition.

[147] In still other aspects of this embodiment, a composition disclosed herein comprises a silicate clay

in an amount of from, e.g., about 0.001% to about 0.005%, 0.001% to about 0.0075%, about 0.001% to

about 0.01%, about 0.001% to about 0.05%, about 0.001% to about 0.075%, about 0.001% to about 0.1%,

about 0.001% to about 0.25%, about 0.001% to about 0.5%, about 0.001% to about 0.75%, about 0.005%

to about 0.0075%, about 0.005% to about 0.01%, about 0.005% to about 0.05%, about 0.005% to about

0.075%, about 0.005% to about 0.1%, about 0.005% to about 0.25%, about 0.005% to about 0.5%, about

0.005% to about 0.75%, about 0.01% to about 0.05%, 0.01% to about 0.075%, about 0.01% to about 0.1%,

about 0.1% to about 0.5%, about 0.1% to about 0.75%, about 0.1% to about 1%, about 0.1% to about 2%,

about 0.1% to about 3%, about 0.1% to about 4%, about 0.1% to about 5%, about 0.1% to about 6%, about

0.1% to about 7%, about 0.1% to about 8%, about 0.1% to about 9%, about 0.1% to about 10%, about

0.25% to about 0.5%, about 0.25% to about 0.75%, about 0.25% to about 1%, about 0.25% to about 2%,

about 0.25% to about 3%, about 0.25% to about 4%, about 0.25% to about 5%, about 0.25% to about 6%,

about 0.25% to about 7%, about 0.25% to about 8%, about 0.25% to about 9%, about 0.25% to about 10%,

about 0.5% to about 0.75%, about 0.5% to about 1%, about 0,5% to about 2%, about 0.5% to about 3%,

about 0.5% to about 4%, about 0.5% to about 5%, about 0.5% to about 6%, about 0.5% to about 7%, about

0.5% to about 8%, about 0.5% to about 9%, about 0.5% to about 10%, about 0.75% to about 1%, about

0.75% to about 2%, about 0.75% to about 3%, about 0.75% to about 4%, about 0.75% to about 5%, about

0.75% to about 6%, about 0.75% to about 7%, about 0.75% to about 8%, about 0.75% to about 9%, about

0.75% to about 10%, about 1% to about 2%, about 1% to about 3%, about 1% to about 4%, about 1% to

about 5%, about 1% to about 6%, about 1% to about 7%, about 1% to about 8%, about 1% to about 9%,

about 1% to about 10%, about 2% to about 3%, about 2% to about 4%, about 2% to about 5%, about 2%

to about 6%, about 2% to about 7%, about 2% to about 8%, about 2% to about 9%, about 2% to about

10% or about 9% to about 10% by weight of the composition.

[148] A preservative preserves the stability of a composition disclosed herein. A preservative can also

prevent the growth of microbial organisms in a composition disclosed herein. Non-limiting examples of a preservative include methylparaben, phenoxyethanol, capryl glycol, glyceryl caprylate, benzoic acid, sorbic acid, gallic acid, and propylparaben.

[149] A composition disclosed herein may be adjusted to any pH that enables a composition disclosed

herein to achieve a desired beneficial effect. In aspects of this embodiment, the pH of a composition

disclosed herein is, e.g., at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least

9, at least 10, or at least 11. In other aspects of this embodiment, the pH of a composition disclosed herein

is, e.g., at most 2, at most 3, at most 4, at most 5, at most 6, at most 7, at most 8, at most 9, at most 10, or

at most 11.

[150] In yet other aspects of this embodiment, the pH of a composition disclosed herein is between, e.g.,

about 2 to about 5, about 2 to about 5.5, about 2 to about 6, about 2 to about 6.5, about 2 to about 7, about

2 to about 7.5, about 2 to about 8, about 2 to about 8.5, about 2 to about 9, about 2.5 to about 5, about 2.5

to about 5.5, about 2.5 to about 6, about 2.5 to about 6.5, about 2.5 to about 7, about 2.5 to about 7.5,

about 2.5 to about 8, about 2.5 to about 8.5, about 2.5 to about 9, about 3 to about 5, about 3 to about 5.5,

about 3 to about 6, about 3 to about 6.5, about 3 to about 7, about 3 to about 7.5, about 3 to about 8, about

3 to about 8.5, about 3 to about 9, about 3.5 to about 5, about 3.5 to about 5.5, about 3.5 to about 6, about

3.5 to about 6.5, about 3.5 to about 7, about 3.5 to about 7.5, about 3.5 to about 8, about 3.5 to about 8.5,

about 3.5 to about 9, about 4 to about 5, about 4 to about 5.5, about 4 to about 6, about 4 to about 6.5,

about 4 to about 7, about 4 to about 7.5, about 4 to about 8, about 4 to about 8.5, about 4 to about 9, about

4.5 to about 5, about 4.5 to about 5.5, about 4.5 to about 6, about 4.5 to about 6.5, about 4.5 to about 7,

about 4.5 to about 7.5, about 4.5 to about 8, about 4.5 to about 8.5, about 4.5 to about 9, about 5 to about

5.5, about 5 to about 6, about 5 to about 6.5, about 5 to about 7, about 5 to about 7.5, about 5 to about 8,

about 5 to about 8.5, about 5 to about 9, about 5.5 to about 6, about 5.5 to about 6.5, about 5.5 to about 7,

about 5.5 to about 7.5, about 5.5 to about 8, about 5.5 to about 8.5, about 5.5 to about 9, about 6 to about

6.5, about 6 to about 7, about 6 to about 7.5, about 6 to about 8, about 6 to about 8.5, about 6 to about 9,

about 6.5 to about 7, about 6.5 to about 7.5, about 6.5 to about 8, about 6.5 to about 8.5, about 6.5 to about

9, about 7 to about 7.5, about 7 to about 8, about 7 to about 8.5, about 7 to about 9, about 7.5 to about 8,

about 7.5 to about 8.5, about 7.5 to about 9, about 8 to about 8.5, about 8 to about 9, or about 8.5 to about

9.

[151] In another embodiment, a calcium oxide powder is used to pH a composition disclosed herein. In

some embodiments, a calcium oxide powder obtained from an organic source like shells from oceanic

organisms. In some embodiment, a calcium oxide powder obtain from an inorganic source like by a

synthetic chemical process. In some embodiments, about 160 ppm of a calcium oxide powder results in a

composition having a pH of about 5.0 to about 5.2. In some embodiments, about 240 ppm of a calcium

oxide powder results in a composition having a pH of about 7.5 to about 7,8. In aspects of this embodiment,

a calcium oxide powder is used to pH a composition disclosed herein to, e.g., at least 2, at least 3, at least

4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, or at least 11. In other aspects of this

embodiment, a calcium oxide powder is used to pH a composition disclosed herein to, e.g., at most 2, at

most 3, at most 4, at most 5, at most 6, at most 7, at most 8, at most 9, at most 10, or at most 11.

[152] In yet other aspects of this embodiment, a calcium oxide powder is used to pH a composition

disclosed herein to, e.g., about 2 to about 5, about 2 to about 5.5, about 2 to about 6, about 2 to about 6.5,

about 2 to about 7, about 2 to about 7.5, about 2 to about 8, about 2 to about 8.5, about 2 to about 9, about

2.5 to about 5, about 2.5 to about 5.5, about 2.5 to about 6, about 2.5 to about 6.5, about 2.5 to about 7,

about 2.5 to about 7.5, about 2.5 to about 8, about 2.5 to about 8.5, about 2.5 to about 9, about 3 to about

5, about 3 to about 5.5, about 3 to about 6, about 3 to about 6.5, about 3 to about 7, about 3 to about 7.5,

about 3 to about 8, about 3 to about 8.5, about 3 to about 9, about 3.5 to about 5, about 3.5 to about 5.5,

about 3.5 to about 6, about 3.5 to about 6.5, about 3.5 to about 7, about 3.5 to about 7.5, about 3.5 to about

8, about 3.5 to about 8.5, about 3.5 to about 9, about 4 to about 5, about 4 to about 5.5, about 4 to about

6, about 4 to about 6.5, about 4 to about 7, about 4 to about 7.5, about 4 to about 8, about 4 to about 8.5,

about 4 to about 9, about 4.5 to about 5, about 4.5 to about 5.5, about 4.5 to about 6, about 4.5 to about

6.5, about 4.5 to about 7, about 4.5 to about 7.5, about 4.5 to about 8, about 4.5 to about 8.5, about 4.5 to

about 9, about 5 to about 5.5, about 5 to about 6, about 5 to about 6.5, about 5 to about 7, about 5 to about

7.5, about 5 to about 8, about 5 to about 8.5, about 5 to about 9, about 5.5 to about 6, about 5.5 to about

6.5, about 5.5 to about 7, about 5.5 to about 7.5, about 5.5 to about 8, about 5.5 to about 8.5, about 5.5 to

about 9, about 6 to about 6.5, about 6 to about 7, about 6 to about 7.5, about 6 to about 8, about 6 to about

8.5, about 6 to about 9, about 6.5 to about 7, about 6.5 to about 7.5, about 6.5 to about 8, about 6.5 to

about 8.5, about 6.5 to about 9, about 7 to about 7.5, about 7 to about 8, about 7 to about 8.5, about 7 to

about 9, about 7.5 to about 8, about 7.5 to about 8.5, about 7.5 to about 9, about 8 to about 8.5, about 8 to

about 9, or about 8.5 to about 9.

[153] A composition disclosed herein is resistant to inactivation when exposed to a bodily fluid. A bodily

fluid is one containing charged molecules and includes, without limitation, blood, and a blood byproduct like

plasma and serum. In one embodiment, a composition disclosed herein is resistant to inactivation when it

shows, e.g., at most 1% inactivation, at most 2% inactivation, at most 3% inactivation, at most 4%

inactivation, at most 5% inactivation, at most 6% inactivation, at most 7% inactivation, at most 8%

inactivation, at most 9% inactivation, or at most 10% inactivation upon exposure to a bodily fluid. In another

embodiment, a composition disclosed herein is resistant to inactivation when it shows, e.g., at most 10%

inactivation, at most 15% inactivation, at most 20% inactivation, at most 25% inactivation, at most 30%

inactivation, at most 35% inactivation, at most 40% inactivation, at most 45% inactivation, at most 50%

inactivation upon exposure to a bodily fluid. In another embodiment, a composition disclosed herein is

resistant to inactivation when it shows, e.g., about 1% to about 10% inactivation, about 1% to about 20%

inactivation, about 1% to about 30% inactivation, about 1% to about 40% inactivation, about 1% to about

50% inactivation, about 5% to about 10% inactivation, about 5% to about 20% inactivation, about 5% to

about 30% inactivation, about 5% to about 40% inactivation, about 5% to about 50% inactivation, about

10% to about 20% inactivation, about 10% to about 30% inactivation, about 10% to about 40% inactivation,

about 10% to about 50% inactivation, 20% to about 30% inactivation, about 20% to about 40% inactivation,

about 20% to about 50% inactivation, about 30% to about 40% inactivation, about 30% to about 50%

inactivation, or about 40% to about 50% inactivation upon exposure to a bodily fluid.

[154] In one embodiment, a composition disclosed herein is resistant to inactivation when it shows, e.g.,

at most 1% degradation, at most 2% degradation, at most 3% degradation, at most 4% degradation, at

most 5% degradation, at most 6% degradation, at most 7% degradation, at most 8% degradation, at most

9% degradation, or at most 10% degradation of hypochlorous acid upon exposure to a bodily fluid. In

another embodiment, a composition disclosed herein is resistant to inactivation when it shows, e.g., at most

10% degradation, at most 15% degradation, at most 20% degradation, at most 25% degradation, at most

30% degradation, at most 35% degradation, at most 40% degradation, at most 45% degradation, at most

50% degradation of hypochlorous acid upon exposure to a bodily fluid. In another embodiment, a

composition disclosed herein is resistant to inactivation when it shows, e.g., about 1% to about 10%

degradation, about 1% to about 20% degradation, about 1% to about 30% degradation, about 1% to about

40% degradation, about 1% to about 50% degradation, about 5% to about 10% degradation, about 5% to

about 20% degradation, about 5% to about 30% degradation, about 5% to about 40% degradation, about

5% to about 50% degradation, about 10% to about 20% degradation, about 10% to about 30% degradation,

about 10% to about 40% degradation, about 10% to about 50% degradation, 20% to about 30% degradation, about 20% to about 40% degradation, about 20% to about 50% degradation, about 30% to

about 40% degradation, about 30% to about 50% degradation, or about 40% to about 50% degradation of

hypochlorous acid upon exposure to a bodily fluid.

[155] A composition disclosed herein is stable. A composition disclosed herein is stable, or has stability,

in two different aspects. First, a composition disclosed herein has a shelf-life stability. Shelf-life stability is

the length of time that a composition disclosed herein may be stored prior to its first use without becoming

unfit for its intended purpose of restraining, inhibiting or otherwise preventing microbial population growth.

Shelf-life stability is the time from when a composition disclosed herein is manufactured and packaged in

a container until the time the composition becomes unfit for its intended purpose while remaining unopened

in the container. Second, a composition disclosed herein has an effective-use stability. Effective-use

stability is the length of time that a composition disclosed herein is effective for its intended purpose of

restraining, inhibiting or otherwise preventing microbial population growth after its first use. Effective-use

stability is the time from when a container storing a composition disclosed herein is first open or

manufactured on site for immediate use until the time the composition becomes unfit for its intended

purpose.

[156] In one embodiment, a composition disclosed herein is stable when it shows, e.g., at most 1%

degradation, at most 2% degradation, at most 3% degradation, at most 4% degradation, at most 5%

degradation, at most 6% degradation, at most 7% degradation, at most 8% degradation, at most 9%

degradation, or at most 10% degradation of hypochlorous acid. In another embodiment, a composition

disclosed herein is stable when it shows, e.g., at most 10% degradation, at most 15% degradation, at most

20% degradation, at most 25% degradation, at most 30% degradation, at most 35% degradation, at most

40% degradation, at most 45% degradation, at most 50% degradation, at most 55% degradation, at most

60% degradation, at most 65% degradation, at most 70% degradation, at most 75% degradation, at most

80% degradation, at most 85% degradation, at most 90% degradation, or at most 95% degradation of

hypochlorous acid. In another embodiment, a composition disclosed herein is stable when it shows, e.g., about 1% to about 10% degradation, about 1% to about 20% degradation, about 1% to about 30% degradation, about 1% to about 40% degradation, about 1% to about 50% degradation, about 1% to about

60% degradation, about 1% to about 70% degradation, about 1% to about 80% degradation, about 1% to

about 90% degradation, about 1% to about 95% degradation, about 5% to about 10% degradation, about

5% to about 20% degradation, about 5% to about 30% degradation, about 5% to about 40% degradation,

about 5% to about 50% degradation, about 5% to about 60% degradation, about 5% to about 70% degradation, about 5% to about 80% degradation, about 5% to about 90% degradation, about 5% to about

95% degradation, about 10% to about 20% degradation, about 10% to about 30% degradation, about 10%

to about 40% degradation, about 10% to about 50% degradation, about 10% to about 60% degradation,

about 10% to about 70% degradation, about 10% to about 80% degradation, about 10% to about 90%

degradation, about 10% to about 95% degradation, 20% to about 30% degradation, about 20% to about

40% degradation, about 20% to about 50% degradation, about 20% to about 60% degradation, about 20%

to about 70% degradation, about 20% to about 80% degradation, about 20% to about 90% degradation,

about 20% to about 95% degradation, about 30% to about 40% degradation, about 30% to about 50%

degradation, about 30% to about 60% degradation, about 30% to about 70% degradation, about 30% to

about 80% degradation, about 30% to about 90% degradation, about 30% to about 95% degradation, about

40% to about 50% degradation, about 40% to about 60% degradation, about 40% to about 70% degradation, about 40% to about 80% degradation, about 40% to about 90% degradation, about 40% to

about 95% degradation, about 50% to about 60% degradation, about 50% to about 70% degradation, about

50% to about 80% degradation, about 50% to about 90% degradation, about 50% to about 95% degradation, about 60% to about 70% degradation, about 60% to about 80% degradation, about 60% to

about 90% degradation, about 60% to about 95% degradation, about 70% to about 80% degradation, about

70% to about 90% degradation, about 70% to about 95% degradation, about 80% to about 90%

degradation, about 80% to about 95% degradation, or about 90% to about 95% degradation of hypochlorous acid.

[157] In one embodiment, a composition disclosed herein is stable when it shows, e.g., at most 1%

degradation, or at most 10% degradation of free available chlorine. In another embodiment, a composition

80% degradation, at most 85% degradation, at most 90% degradation, or at most 95% degradation of free

available chlorine. In another embodiment, a composition disclosed herein is stable when it shows, e.g.,

about 1% to about 10% degradation, about 1% to about 20% degradation, about 1% to about 30% degradation, about 1% to about 40% degradation, about 1% to about 50% degradation, about 1% to about

5% to about 20% degradation, about 5% to about 30% degradation, about 5% to about 40% degradation, about 5% to about 50% degradation, about 5% to about 60% degradation, about 5% to about 70% degradation, about 5% to about 80% degradation, about 5% to about 90% degradation, about 5% to about

70% to about 90% degradation, about 70% to about 95% degradation, about 80% to about 90% degradation, about 80% to about 95% degradation, or about 90% to about 95% degradation of free

available chlorine.

[158] In aspects of this embodiment, a composition disclosed herein has an effective-use stability of e.g.,

about 1 day, about 2 days, 3 days, 4 days, 5 days, 6, days, 7 days, 8 days, 9 days, 10 days, 11 days, 12

days, 13 days, 14 days, 15 days, about 20 days, about 25 days, or about 30 days. In other aspects of this

embodiment, a composition disclosed herein has an effective-use stability of, e.g., at least 1 day, at least 2

days, at least 3 days, at least 4 days, at least 5 days, at least 6, days, at least 7 days, at least 8 days, at

least 9 days, at least 10 days, at least 11 days, at least 12 days, at least 13 days, at least 14 days, at least

15 days, at least 20 days, at least 25 days, or at least 30 days. In yet other aspects of this embodiment, a

composition disclosed herein has an effective-use stability of, e.g., at most 1 day, at most 2 days, at most

3 days, at most 4 days, at most 5 days, at most 6, days, at most 7 days, at most 8 days, at most 9 days, at

most 10 days, at most 11 days, at most 12 days, at most 13 days, at most 14 days, at most 15 days, at

most 20 days, at most 25 days, or at most 30 days. In still other aspects of this embodiment, a composition

disclosed herein has an effective-use stability of, e.g., about 1 hour to about 4 hours, about 1 hour to about

8 hours, about 1 hour to about 12 hours, about 1 hour to about 16 hours, about 1 hour to about 20 hours,

or about 1 hour to about 24 hours.

[159] In still aspects of this embodiment, a composition disclosed herein has an effective-use stability of,

e.g., about 1 day to about 2 days, about 1 day to about 3 days, about 1 day to about 4 days, about 1 day

to about 5 days, about 1 day to about 6 days, about 1 day to about 7 days, about 1 day to about 8 days,

about 1 day to about 9 days, about 1 day to about 10 days, about 1 day to about 11 days, about 1 day to

about 12 days, about 1 day to about 13 days, about 1 day to about 14 days, about 1 day to about 15 days,

WO wo 2020/252433 PCT/US2020/037665 75

about 1 day to about 20 days, about 1 day to about 25 days, about 1 day to about 30 days, about 2 days

to about 3 days, about 2 days to about 4 days, about 2 days to about 5 days, about 2 days to about 6 days,

about 2 days to about 7 days, about 2 days to about 8 days, about 2 days to about 9 days, about 2 days to

about 10 days, about 2 days to about 11 days, about 2 days to about 12 days, about 2 days to about 13

days, about 2 days to about 14 days, about 2 days to about 15 days, about 2 days to about 20 days, about

2 days to about 25 days, about 2 days to about 30 days, about 3 days to about 4 days, about 3 days to

about 5 days, about 3 days to about 6 days, about 3 days to about 7 days, about 3 days to about 8 days,

about 3 days to about 9 days, about 3 days to about 10 days, about 3 days to about 11 days, about 3 days

to about 12 days, about 3 days to about 13 days, about 3 days to about 14 days, about 3 days to about 15

days, about 3 days to about 20 days, about 3 days to about 25 days, about 3 days to about 30 days, about

4 days to about 5 days, about 4 days to about 6 days, about 4 days to about 7 days, about 4 days to about

8 days, about 4 days to about 9 days, about 4 days to about 10 days, about 4 days to about 11 days, about

4 days to about 12 days, about 4 days to about 13 days, about 4 days to about 14 days, about 4 days to

about 15 days, about 4 days to about 20 days, about 4 days to about 25 days, about 4 days to about 30

days, about 5 days to about 6 days, about 5 days to about 7 days, about 5 days to about 8 days, about 5

days to about 9 days, about 5 days to about 10 days, about 5 days to about 11 days, about 5 days to about

12 days, about 5 days to about 13 days, about 5 days to about 14 days, about 5 days to about 15 days,

about 5 days to about 20 days, about 5 days to about 25 days, about 5 days to about 30 days, about 6 days

to about 7 days, about 6 days to about 8 days, about 6 days to about 9 days, about 6 days to about 10

days, about 6 days to about 11 days, about 6 days to about 12 days, about 6 days to about 13 days, about

6 days to about 14 days, about 6 days to about 15 days, about 6 days to about 20 days, about 6 days to

about 25 days, about 6 days to about 30 days, about 7 days to about 8 days, about 7 days to about 9 days,

about 7 days to about 10 days, about 7 days to about 11 days, about 7 days to about 12 days, about 7 days

to about 13 days, about 7 days to about 14 days, about 7 days to about 15 days, about 7 days to about 20

days, about 7 days to about 25 days, about 7 days to about 30 days, about 8 days to about 9 days, about

8 days to about 10 days, about 8 days to about 11 days, about 8 days to about 12 days, about 8 days to

about 13 days, about 8 days to about 14 days, about 8 days to about 15 days, about 8 days to about 20

days, about 8 days to about 25 days, about 8 days to about 30 days, about 9 days to about 10 days, about

9 days to about 11 days, about 9 days to about 12 days, about 9 days to about 13 days, about 9 days to

about 14 days, about 9 days to about 15 days, about 9 days to about 20 days, about 9 days to about 25

days, about 9 days to about 30 days, about 10 days to about 11 days, about 10 days to about 12 days,

about 10 days to about 13 days, about 10 days to about 14 days, about 10 days to about 15 days, about

10 days to about 20 days, about 10 days to about 25 days, about 10 days to about 30 days, about 11 days

to about 12 days, about 11 days to about 13 days, about 11 days to about 14 days, about 11 days to about

15 days, about 11 days to about 20 days, about 11 days to about 25 days, about 11 days to about 30 days,

about 12 days to about 13 days, about 12 days to about 14 days, about 12 days to about 15 days, about

12 days to about 20 days, about 12 days to about 25 days, about 12 days to about 30 days, about 13 days

to about 14 days, about 13 days to about 15 days, about 13 days to about 20 days, about 13 days to about

25 days, about 13 days to about 30 days, about 14 days to about 15 days, about 14 days to about 20 days,

about 14 days to about 25 days, about 14 days to about 30 days, about 15 days to about 20 days, about

15 days to about 25 days, about 15 days to about 30 days, about 20 days to about 25 days, about 20 days

to about 30 days, or about 25 days to about 30 days.

[160] In other aspects of this embodiment, a composition disclosed herein has an effective-use stability

of, e.g., about 1 week, about 2 weeks, 3 weeks, 4 weeks, about 5 weeks, about 6 weeks, about 7 weeks,

about 8 weeks, about 9 weeks, about 10 weeks, about 11 weeks, or about 12 weeks. In yet other aspects

of this embodiment, a composition disclosed herein has an effective-use stability of, e.g., at least 1 week,

at least 2 weeks, at least 3 weeks, at least 4 weeks, at least 5 weeks, at least 6 weeks, at least 7 weeks,

at least 8 weeks, at least 9 weeks, at least 10 weeks, at least 11 weeks, or at least 12 weeks. In still other

aspects of this embodiment, a composition disclosed herein has an effective-use stability of, e.g., at most

1 week, at most 2 weeks, at most 3 weeks, at most 4 weeks, at most 5 weeks, at most 6 weeks, at most 7

weeks, at most 8 weeks, at most 9 weeks, at most 10 weeks, at most 11 weeks, or at most 12 weeks. In

aspects of this embodiment, a composition disclosed herein has an effective-use stability of, e.g., about 1

week to about 2 weeks, about 1 week to about 3 weeks, about 1 week to about 4 weeks, about 1 week to

about 5 weeks, about 1 week to about 6 weeks, about 1 week to about 7 weeks, about 1 week to about 8

weeks, about 1 week to about 9 weeks, about 1 week to about 10 weeks, about 1 week to about 11 weeks,

about 1 week to about 12 weeks, about 2 weeks to about 3 weeks, about 2 weeks to about 4 weeks, about

2 weeks to about 5 weeks, about 2 weeks to about 6 weeks, about 2 weeks to about 7 weeks, about 2

weeks to about 8 weeks, about 2 weeks to about 9 weeks, about 2 weeks to about 10 weeks, about 2

weeks to about 11 weeks, about 2 weeks to about 12 weeks, about 3 weeks to about 4 weeks, about 3

weeks to about 5 weeks, about 3 weeks to about 6 weeks, about 3 weeks to about 7 weeks, about 3 weeks

to about 8 weeks, about 3 weeks to about 9 weeks, about 3 weeks to about 10 weeks, about 3 weeks to

about 11 weeks, about 3 weeks to about 12 weeks, about 4 weeks to about 5 weeks, about 4 weeks to

about 6 weeks, about 4 weeks to about 7 weeks, about 4 weeks to about 8 weeks, about 4 weeks to about

9 weeks, about 4 weeks to about 10 weeks, about 4 weeks to about 11 weeks, about 4 weeks to about 12

weeks, about 5 weeks to about 6 weeks, about 5 weeks to about 7 weeks, about 5 weeks to about 8 weeks,

about 5 weeks to about 9 weeks, about 5 weeks to about 10 weeks, about 5 weeks to about 11 weeks,

about 5 weeks to about 12 weeks, about 6 weeks to about 7 weeks, about 6 weeks to about 8 weeks, about

6 weeks to about 9 weeks, about 6 weeks to about 10 weeks, about 6 weeks to about 11 weeks, about 6

weeks to about 12 weeks, about 7 weeks to about 8 weeks, about 7 weeks to about 9 weeks, about 7

weeks to about 10 weeks, about 7 weeks to about 11 weeks, about 7 weeks to about 12 weeks, about 8

weeks to about 9 weeks, about 8 weeks to about 10 weeks, about 8 weeks to about 11 weeks, about 8

weeks to about 12 weeks, about 9 weeks to about 10 weeks, about 9 weeks to about 11 weeks, about 9

weeks to about 12 weeks, about 10 weeks to about 11 weeks, about 10 weeks to about 12 weeks, or about

11 weeks to about 12 weeks.

[161] In other aspects of this embodiment, a composition disclosed herein has a shelf-life stability of,

e.g., about 1 month, about 2 months, about 3 months, about 4 months, about 5 months, about 6, months,

about 7 months, about 8 months, about 9 months, about 10 months, about 11 months, about 12 months,

about 13 months, about 14 months, about 15 months, about 16 months, about 17 months, about 18 months,

about 19 months, about 20 months, about 21 months, about 22 months, about 23 months, or about 24

WO wo 2020/252433 PCT/US2020/037665 77

months. In yet other aspects of this embodiment, a composition disclosed herein has a shelf-life stability

o, e.g., at least 1 month, at least 2 months, at least 3 months, at least 4 months, at least 5 months, at least

6, months, at least 7 months, at least 8 months, at least 9 months, at least 10 months, at least 11 months,

at least 12 months, at least 13 months, at least 14 months, at least 15 months, at least 16 months, at least

17 months, at least 18 months, at least 19 months, at least 20 months, at least 21 months, at least 22

months, at least 23 months, or at least 24 months. In still other aspects of this embodiment, a composition

disclosed herein has a shelf-life stability of, e.g., at most 1 month, at most 2 months, at most 3 months, at

most 4 months, at most 5 months, at most 6, months, at most 7 months, at most 8 months, at most 9

months, at most 10 months, at most 11 months, at most 12 months, at most 13 months, at most 14 months,

at most 15 months, at most 16 months, at most 17 months, at most 18 months, at most 19 months, at most

20 months, at most 21 months, at most 22 months, at most 23 months, or at most 24 months.

[162] In aspects of this embodiment, a composition disclosed herein has a shelf-life stability of, e.g.,

about 1 month to about 2 months, about 1 month to about 3 months, about 1 month to about 4 months,

about 1 month to about 5 months, about 1 month to about 6 months, about 1 month to about 7 months,

about 1 month to about 8 months, about 1 month to about 9 months, about 1 month to about 10 months,

about 1 month to about 11 months, about 1 month to about 12 months, about 1 month to about 13 months,

about 1 month to about 14 months, about 1 month to about 15 months, about 1 month to about 16 months,

about 1 month to about 17 months, about 1 month to about 18 months, about 1 month to about 19 months,

about 1 month to about 20 months, about 1 month to about 21 months, about 1 month to about 22 months,

about 1 month to about 23 months, about 1 month to about 24 months, about 2 months to about 3 months,

about 2 months to about 4 months, about 2 months to about 5 months, about 2 months to about 6 months,

about 2 months to about 7 months, about 2 months to about 8 months, about 2 months to about 9 months,

about 2 months to about 10 months, about 2 months to about 11 months, about 2 months to about 12

months, about 2 months to about 13 months, about 2 months to about 14 months, about 2 months to about

15 months, about 2 months to about 16 months, about 2 months to about 17 months, about 2 months to

about 18 months, about 2 months to about 19 months, about 2 months to about 20 months, about 2 months

to about 21 months, about 2 months to about 22 months, about 2 months to about 23 months, about 2

months to about 24 months, about 3 months to about 4 months, about 3 months to about 5 months, about

3 months to about 6 months, about 3 months to about 7 months, about 3 months to about 8 months, about

3 months to about 9 months, about 3 months to about 10 months, about 3 months to about 11 months,

about 3 months to about 12 months, about 3 months to about 13 months, about 3 months to about 14

months, about 3 months to about 15 months, about 3 months to about 16 months, about 3 months to about

17 months, about 3 months to about 18 months, about 3 months to about 19 months, about 3 months to

about 20 months, about 3 months to about 21 months, about 3 months to about 22 months, about 3 months

to about 23 months, about 3 months to about 24 months, about 4 months to about 5 months, about 4

months to about 6 months, about 4 months to about 7 months, about 4 months to about 8 months, about 4

months to about 9 months, about 4 months to about 10 months, about 4 months to about 11 months, about

4 months to about 12 months, about 4 months to about 13 months, about 4 months to about 14 months,

about 4 months to about 15 months, about 4 months to about 16 months, about 4 months to about 17

months, about 4 months to about 18 months, about 4 months to about 19 months, about 4 months to about

WO wo 2020/252433 PCT/US2020/037665 78

20 months, about 4 months to about 21 months, about 4 months to about 22 months, about 4 months to

about 23 months, about 4 months to about 24 months, about 5 months to about 6 months, about 5 months

to about 7 months, about 5 months to about 8 months, about 5 months to about 9 months, about 5 months

to about 10 months, about 5 months to about 11 months, about 5 months to about 12 months, about 5

months to about 13 months, about 5 months to about 14 months, about 5 months to about 15 months,

about 5 months to about 16 months, about 5 months to about 17 months, about 5 months to about 18

months, about 5 months to about 19 months, about 5 months to about 20 months, about 5 months to about

21 months, about 5 months to about 22 months, about 5 months to about 23 months, about 5 months to

about 24 months, about 6 months to about 7 months, about 6 months to about 8 months, about 6 months

to about 9 months, about 6 months to about 10 months, about 6 months to about 11 months, about 6 months to about 12 months, about 6 months to about 13 months, about 6 months to about 14 months,

about 6 months to about 15 months, about 6 months to about 16 months, about 6 months to about 17

months, about 6 months to about 18 months, about 6 months to about 19 months, about 6 months to about

20 months, about 6 months to about 21 months, about 6 months to about 22 months, about 6 months to

about 23 months, about 6 months to about 24 months, about 7 months to about 8 months, about 7 months

to about 9 months, about 7 months to about 10 months, about 7 months to about 11 months, about 7

months to about 12 months, about 7 months to about 13 months, about 7 months to about 14 months,

about 7 months to about 15 months, about 7 months to about 16 months, about 7 months to about 17

months, about 7 months to about 18 months, about 7 months to about 19 months, about 7 months to about

20 months, about 7 months to about 21 months, about 7 months to about 22 months, about 7 months to

about 23 months, about 7 months to about 24 months, about 8 months to about 9 months, about 8 months

to about 10 months, about 8 months to about 11 months, about 8 months to about 12 months, about 8

months to about 13 months, about 8 months to about 14 months, about 8 months to about 15 months,

about 8 months to about 16 months, about 8 months to about 17 months, about 8 months to about 18

months, about 8 months to about 19 months, about 8 months to about 20 months, about 8 months to about

21 months, about 8 months to about 22 months, about 8 months to about 23 months, about 8 months to

about 24 months, about 9 months to about 10 months, about 9 months to about 11 months, about 9 months

to about 12 months, about 9 months to about 13 months, about 9 months to about 14 months, about 9

months to about 15 months, about 9 months to about 16 months, about 9 months to about 17 months,

about 9 months to about 18 months, about 9 months to about 19 months, about 9 months to about 20

months, about 9 months to about 21 months, about 9 months to about 22 months, about 9 months to about

23 months, about 9 months to about 24 months, about 10 months to about 11 months, about 10 months to

about 12 months, about 10 months to about 13 months, about 10 months to about 14 months, about 10

months to about 15 months, about 10 months to about 16 months, about 10 months to about 17 months,

about 10 months to about 18 months, about 10 months to about 19 months, about 10 months to about 20

months, about 10 months to about 21 months, about 10 months to about 22 months, about 10 months to

about 23 months, about 10 months to about 24 months, about 11 months to about 12 months, about 11

months to about 13 months, about 11 months to about 14 months, about 11 months to about 15 months,

about 11 months to about 16 months, about 11 months to about 17 months, about 11 months to about 18

months, about 11 months to about 19 months, about 11 months to about 20 months, about 11 months to

about 21 months, about 11 months to about 22 months, about 11 months to about 23 months, about 11 months to about 24 months, about 12 months to about 13 months, about 12 months to about 14 months, about 12 months to about 15 months, about 12 months to about 16 months, about 12 months to about 17 months, about 12 months to about 18 months, about 12 months to about 19 months, about 12 months to about 20 months, about 12 months to about 21 months, about 12 months to about 22 months, about 12 months to about 23 months, about 12 months to about 24 months, about 13 months to about 14 months, about 13 months to about 15 months, about 13 months to about 16 months, about 13 months to about 17 months, about 13 months to about 18 months, about 13 months to about 19 months, about 13 months to about 20 months, about 13 months to about 21 months, about 13 months to about 22 months, about 13 months to about 23 months, about 13 months to about 24 months, about 14 months to about 15 months, about 14 months to about 16 months, about 14 months to about 17 months, about 14 months to about 18 months, about 14 months to about 19 months, about 14 months to about 20 months, about 14 months to about 21 months, about 14 months to about 22 months, about 14 months to about 23 months, about 14 months to about 24 months, about 15 months to about 16 months, about 15 months to about 17 months, about 15 months to about 18 months, about 15 months to about 19 months, about 15 months to about 20 months, about 15 months to about 21 months, about 15 months to about 22 months, about 15 months to about 23 months, about 15 months to about 24 months, about 16 months to about 17 months, about 16 months to about 18 months, about 16 months to about 19 months, about 16 months to about 20 months, about 16 months to about 21 months, about 16 months to about 22 months, about 16 months to about 23 months, about 16 months to about 24 months, about 17 months to about 18 months, about 17 months to about 19 months, about 17 months to about 20 months, about 17 months to about 21 months, about 17 months to about 22 months, about 17 months to about 23 months, about 17 months to about 24 months, about 18 months to about 19 months, about 18 months to about 20 months, about 18 months to about 21 months, about 18 months to about 22 months, about 18 months to about 23 months, about 18 months to about 24 months, about 19 months to about 20 months, about 19 months to about 21 months, about 19 months to about 22 months, about 19 months to about 23 months, about 19 months to about 24 months, about 20 months to about 22 months, about 20 months to about 23 months, about 20 months to about 24 months, about 21 months to about 22 months, about 21 months to about 23 months, about 21 months to about 24 months, about 22 months to about 23 months, about 22 months to about 24 months, or about 23 months to about 24 months.

[163] In other aspects of this embodiment, a composition disclosed herein has a shelf-life stability of,

e.g., about 1 year, about 2 years, about 3 years, about 4 years, or about 5 years. In yet other aspects of

this embodiment, a composition disclosed herein has a shelf-life stability of, e.g., at least 1 year, at least 2

years, at least 3 years, at least 4 years, or at least 5 years. In still other aspects of this embodiment, a

composition disclosed herein has a shelf-life stability of, e.g., at most 1 year, at most 2 years, at most 3

years, at most 4 years, or at most 5 years. In other aspects of this embodiment, a composition disclosed

herein has a shelf-life stability of, e.g., about 1 year to about 2 years, about 1 year to about 3 years, about

1 year to about 4 years, about 1 year to about 5 years, about 2 years to about 3 years, about 2 years to

about 4 years, about 2 years to about 5 years, about 3 years to about 4 years, about 3 years to about 5

years, or about 4 years to about 5 years.

[164] A composition disclosed herein can be formulated into any form that enables application of a

composition disclosed herein in a manner that achieves a desired beneficial effect. In one embodiment, a

composition disclosed herein can be formulated into, e.g., a single-phase formulation or a biphasic

formulation comprising a medium phase and a dispersed phase. In another embodiment, a composition

disclosed herein can be formulated into, e.g., a liquid composition, a colloidal composition, a semi-solid

composition, or a solid composition. In another embodiment, a composition disclosed herein can be

formulated into, e.g., a liquid aerosol, a foam, an emulsion, a gel, a sol, or a solid sol. In another

embodiment, a composition disclosed herein can be formulated into, e.g., a spray, a liquid aerosol, a soap,

or a suspension.

[165] In an embodiment, a composition disclosed herein comprises 5 ppm to 100 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 10

ppm to 90 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 15 ppm to 85 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 20 ppm to 80 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 25 ppm to

75 ppm hypochlorous acid or free available chlorine.

[166] In an embodiment, a composition disclosed herein comprises 5 ppm to 90 ppm hypochlorous acid

ppm to 85 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 15 ppm to 80 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 20 ppm to 75 ppm hypochlorous acid or free

70 ppm hypochlorous acid or free available chlorine.

[167] In an embodiment, a composition disclosed herein comprises 5 ppm to 80 ppm hypochlorous acid

ppm to 75 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 15 ppm to 70 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 20 ppm to 65 ppm hypochlorous acid or free

60 ppm hypochlorous acid or free available chlorine.

[168] In an embodiment, a composition disclosed herein comprises 5 ppm to 70 ppm hypochlorous acid

ppm to 65 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 15 ppm to 60 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 20 ppm to 55 ppm hypochlorous acid or free

50 ppm hypochlorous acid or free available chlorine.

wo 2020/252433 WO PCT/US2020/037665 81

[169] In an embodiment, a composition disclosed herein comprises 5 ppm to 60 ppm hypochlorous acid

ppm to 55 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 15 ppm to 50 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 20 ppm to 45 ppm hypochlorous acid or free

40 ppm hypochlorous acid or free available chlorine.

[170] In an embodiment, a composition disclosed herein comprises 5 ppm to 50 ppm hypochlorous acid

ppm to 45 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 15 ppm to 40 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 20 ppm to 35 ppm hypochlorous acid or free

30 ppm hypochlorous acid or free available chlorine.

[171] In an embodiment, a composition disclosed herein comprises 90 ppm to 140 ppm hypochlorous

acid or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises

95 ppm to 135 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a

composition disclosed herein comprises 100 ppm to 130 ppm hypochlorous acid or free available chlorine.

In an aspect of this embodiment, a composition disclosed herein comprises 105 ppm to 125 ppm

hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition disclosed

herein comprises 110 ppm to 120 ppm hypochlorous acid or free available chlorine. In an aspect of this

embodiment, a composition disclosed herein comprises 115 ppm hypochlorous acid or free available

chlorine.

[172] In an embodiment, a composition disclosed herein comprises 75 ppm to 125 ppm hypochlorous

80 ppm to 120 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a

composition disclosed herein comprises 85 ppm to 115 ppm hypochlorous acid or free available chlorine.

In an aspect of this embodiment, a composition disclosed herein comprises 90 ppm to 110 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition disclosed

herein comprises 95 ppm to 105 ppm hypochlorous acid or free available chlorine. In an aspect of this

embodiment, a composition disclosed herein comprises 100 ppm hypochlorous acid or free available

chlorine.

[173] In an embodiment, a composition disclosed herein comprises 50 ppm to 100 ppm hypochlorous

55 ppm to 95 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a

composition disclosed herein comprises 60 ppm to 90 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 65 ppm to 85 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises

70 ppm to 80 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a

composition disclosed herein comprises 75 ppm hypochlorous acid or free available chlorine.

[174] In an embodiment, a composition disclosed herein comprises 45 ppm to 95 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 50

disclosed herein comprises 55 ppm to 85 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 60 ppm to 80 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 65 ppm to

75 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 70 ppm hypochlorous acid or free available chlorine.

[175] In an embodiment, a composition disclosed herein comprises 40 ppm to 90 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 45

disclosed herein comprises 50 ppm to 80 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 55 ppm to 75 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 60 ppm to

70 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 65 ppm hypochlorous acid or free available chlorine.

[176] In an embodiment, a composition disclosed herein comprises 25 ppm to 85 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 40

ppm to 80 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 45 ppm to 75 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 50 ppm to 70 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 55 ppm to

65 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 60 ppm hypochlorous acid or free available chlorine.

[177] In an embodiment, a composition disclosed herein comprises 30 ppm to 80 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 35

disclosed herein comprises 40 ppm to 70 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 45 ppm to 65 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 50 ppm to

60 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 55 ppm hypochlorous acid or free available chlorine.

[178] In an embodiment, a composition disclosed herein comprises 25 ppm to 75 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 30

ppm to 70 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 35 ppm to 65 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 40 ppm to 60 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 45 ppm to

55 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 50 ppm hypochlorous acid or free available chlorine.

[179] In an embodiment, a composition disclosed herein comprises 20 ppm to 70 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 25

disclosed herein comprises 30 ppm to 60 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 35 ppm to 55 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 40 ppm to

50 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 45 ppm hypochlorous acid or free available chlorine.

[180] In an embodiment, a composition disclosed herein comprises 15 ppm to 65 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 20

ppm to 60 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 25 ppm to 55 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 30 ppm to 50 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 35 ppm to

45 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 40 ppm hypochlorous acid or free available chlorine.

[181] In an embodiment, a composition disclosed herein comprises 10 ppm to 60 ppm hypochlorous acid

or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 15

disclosed herein comprises 20 ppm to 50 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 25 ppm to 45 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 30 ppm to

40 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 35 ppm hypochlorous acid or free available chlorine.

[182] In an embodiment, a composition disclosed herein comprises 5 ppm to 55 ppm hypochlorous acid

ppm to 50 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 15 ppm to 45 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 20 ppm to 40 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 25 ppm to

35 ppm hypochlorous acid or free available chlorine. In an aspect of this embodiment, a composition

disclosed herein comprises 30 ppm hypochlorous acid or free available chlorine.

[183] In an aspect of this embodiment, a composition disclosed herein comprises 1 ppm to 50 ppm

herein comprises 5 ppm to 45 ppm hypochlorous acid or free available chlorine. In an aspect of this

embodiment, a composition disclosed herein comprises 10 ppm to 40 ppm hypochlorous acid or free

available chlorine. In an aspect of this embodiment, a composition disclosed herein comprises 15 ppm to

disclosed herein comprises 20 ppm to 30 ppm hypochlorous acid or free available chlorine. In an aspect

of this embodiment, a composition disclosed herein comprises 25 ppm hypochlorous acid or free available

chlorine.

[184] In an embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm hypochlorous acid

or free available chlorine, and 1 ppm to 200 ppm one or more metal salts. In an aspect of this embodiment,

a composition disclosed herein comprises 1 ppm to 100 ppm hypochlorous acid or free available chlorine,

and 1 ppm to 50 ppm of a copper salt, 1 ppm to 50 ppm of a zinc salt, 1 ppm to 50 ppm of an iron salt, or

any combination thereof. In an aspect of this embodiment, a composition disclosed herein comprises 5

ppm to 75 ppm hypochlorous acid or free available chlorine, and 5 ppm to 40 ppm of a copper salt, 5 ppm

to 40 ppm of a zinc salt and 5 ppm to 40 ppm of an iron salt. In an aspect of this embodiment, a composition

disclosed herein comprises 40 ppm to 60 ppm hypochlorous acid or free available chlorine, and 10 ppm to

30 ppm of a copper salt, 10 ppm to 30 ppm of a zinc salt and 10 ppm to 30 ppm of an iron salt. In an aspect

of this embodiment, a composition disclosed herein comprises 50 ppm hypochlorous acid or free available

chlorine, and 20 ppm of a copper salt, 20 ppm of a zinc salt and 20 ppm of an iron salt.

[185] In an embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm hypochlorous acid

and 1 ppm to 50 ppm of a copper salt, 1 ppm to 50 ppm of a zinc salt, 1 ppm to 50 ppm of a calcium salt,

or any combination thereof. In an aspect of this embodiment, a composition disclosed herein comprises 5

to 40 ppm of a zinc salt and 5 ppm to 40 ppm of a calcium salt. In an aspect of this embodiment, a

composition disclosed herein comprises 40 ppm to 60 ppm hypochlorous acid or free available chlorine,

and 10 ppm to 30 ppm of a copper salt, 10 ppm to 30 ppm of a zinc salt and 10 ppm to 30 ppm of a calcium

salt. In an aspect of this embodiment, a composition disclosed herein comprises 50 ppm hypochlorous

acid or free available chlorine, and 20 ppm of a copper salt, 20 ppm of a zinc salt and 20 ppm of a calcium

salt.

[186] In an aspect of this embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm

hypochlorous acid or free available chlorine, and 1 ppm to 50 ppm of a copper chloride, 1 ppm to 50 ppm

PCT/US2020/037665 85

of a zinc chloride, 1 ppm to 50 ppm of an iron chloride, or any combination thereof. In an aspect of this

embodiment, a composition disclosed herein comprises 5 ppm to 75 ppm hypochlorous acid or free

available chlorine, and 5 ppm to 40 ppm of a copper chloride, 5 ppm to 40 ppm of a zinc chloride and 5

ppm to 40 ppm of an iron chloride. In an aspect of this embodiment, a composition disclosed herein

comprises 40 ppm to 60 ppm hypochlorous acid or free available chlorine, and 10 ppm to 30 ppm of a

copper chloride, 10 ppm to 30 ppm of a zinc chloride and 10 ppm to 30 ppm of an iron chloride. In an

aspect of this embodiment, a composition disclosed herein comprises 50 ppm hypochlorous acid or free

available chlorine, and 20 ppm of a copper chloride, 20 ppm of a zinc chloride and 20 ppm of an iron

chloride. The iron chloride can be iron (II) chloride or iron (III) chloride.

[187] In an aspect of this embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm

of a zinc chloride, 1 ppm to 50 ppm of a calcium oxide salt, or any combination thereof. In an aspect of this

ppm to 40 ppm of a calcium oxide salt. In an aspect of this embodiment, a composition disclosed herein

copper chloride, 10 ppm to 30 ppm of a zinc chloride and 10 ppm to 30 ppm of a calcium oxide salt. In an

available chlorine, and 20 ppm of a copper chloride, 20 ppm of a zinc chloride and 20 ppm of a calcium

oxide salt.

[188] In an embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm hypochlorous

acid or free available chlorine, and 0.1 ppm to 20 ppm one or more metal salts. In an aspect of this

embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm hypochlorous acid or free

available chlorine, and 0.1 ppm to 5 ppm of a copper salt, 0.1 ppm to 5 ppm of a zinc salt, 0.1 ppm to 5

ppm of an iron salt, or any combination thereof. In an aspect of this embodiment, a composition disclosed

herein comprises 0.5 ppm to 7.5 ppm hypochlorous acid or free available chlorine, and 0.5 ppm to 4 ppm

of a copper salt, 0.5 ppm to 4 ppm of a zinc salt and 0.5 ppm to 4 ppm of an iron salt. In an aspect of this

embodiment, a composition disclosed herein comprises 4 ppm to 6 ppm hypochlorous acid or free available

chlorine, and 1 ppm to 3 ppm of a copper salt, 1 ppm to 3 ppm of a zinc salt and 1 ppm to 3 ppm of an iron

salt. In an aspect of this embodiment, a composition disclosed herein comprises 5 ppm hypochlorous acid

or free available chlorine, and 2 ppm of a copper salt, 2 ppm of a zinc salt and 2 ppm of an iron salt.

[189] In an embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm hypochlorous

ppm of a calcium salt, or any combination thereof. In an aspect of this embodiment, a composition

disclosed herein comprises 0.5 ppm to 7.5 ppm hypochlorous acid or free available chlorine, and 0.5 ppm

to 4 ppm of a copper salt, 0.5 ppm to 4 ppm of a zinc salt and 0.5 ppm to 4 ppm of a calcium salt. In an

PCT/US2020/037665 86

aspect of this embodiment, a composition disclosed herein comprises 4 ppm to 6 ppm hypochlorous acid

or free available chlorine, and 1 ppm to 3 ppm of a copper salt, 1 ppm to 3 ppm of a zinc salt and 1 ppm to

3 ppm of a calcium salt. In an aspect of this embodiment, a composition disclosed herein comprises 5

ppm hypochlorous acid or free available chlorine, and 2 ppm of a copper salt, 2 ppm of a zinc salt and 2

ppm of a calcium salt.

[190] In an aspect of this embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm

hypochlorous acid or free available chlorine, and 0.1 ppm to 5 ppm of a copper chloride, 0.1 ppm to 5 ppm

of a zinc chloride, 0.1 ppm to 5 ppm of an iron chloride, or any combination thereof. In an aspect of this

embodiment, a composition disclosed herein comprises 0.5 ppm to 7.5 ppm hypochlorous acid or free

available chlorine, and 0.5 ppm to 4 ppm of a copper chloride, 0.5 ppm to 4 ppm of a zinc chloride and 0.5

ppm to 4 ppm of an iron chloride. In an aspect of this embodiment, a composition disclosed herein

comprises 4 ppm to 6 ppm hypochlorous acid or free available chlorine, and 1 ppm to 3 ppm of a copper

chloride, 1 ppm to 3 ppm of a zinc chloride and 1 ppm to 3 ppm of an iron chloride. In an aspect of this

embodiment, a composition disclosed herein comprises 5 ppm hypochlorous acid or free available chlorine,

and 2 ppm of a copper chloride, 2 ppm of a zinc chloride and 2 ppm of an iron chloride. The iron chloride

can be iron (II) chloride or iron (III) chloride.

[191] In an aspect of this embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm

of a zinc chloride, 0.1 ppm to 5 ppm of a calcium oxide salt, or any combination thereof. In an aspect of

this embodiment, a composition disclosed herein comprises 0.5 ppm to 7.5 ppm hypochlorous acid or free

ppm to 4 ppm of a calcium oxide salt. In an aspect of this embodiment, a composition disclosed herein

chloride, 1 ppm to 3 ppm of a zinc chloride and 1 ppm to 3 ppm of a calcium oxide salt. In an aspect of this

and 2 ppm of a copper chloride, 2 ppm of a zinc chloride and 2 ppm of a calcium oxide salt.

[192] In an embodiment, a composition disclosed herein comprises 0.01 ppm to 5 ppm hypochlorous

acid or free available chlorine, and 0.01 ppm to 5 ppm one or more metal salts. In an aspect of this

embodiment, a composition disclosed herein comprises 0.05 ppm to 1 ppm hypochlorous acid or free

available chlorine, and 0.01 ppm to 2 ppm one or more metal salts. In an aspect of this embodiment, a

composition disclosed herein comprises 0.01 ppm to 1 ppm hypochlorous acid or free available chlorine,

and 0.01 ppm to 0.5 ppm of a copper salt, 0.01 ppm to 0.5 ppm of a zinc salt, 0.01 ppm to 0.5 ppm of an

iron salt, or any combination thereof. In an aspect of this embodiment, a composition disclosed herein

comprises 0.05 ppm to 0.75 ppm hypochlorous acid or free available chlorine, and 0.05 ppm to 0.4 ppm of

a copper salt, 0.05 ppm to 0.4 ppm of a zinc salt and 0.05 ppm to 0.4 ppm of an iron salt. In an aspect of

this embodiment, a composition disclosed herein comprises 0.4 ppm to 0.6 ppm hypochlorous acid or free

available chlorine, and 0.1 ppm to 0.3 ppm of a copper salt, 0.1 ppm to 0.3 ppm of a zinc salt and 0.1 ppm

to 0.3 ppm of an iron salt. In an aspect of this embodiment, a composition disclosed herein comprises 0.5 ppm hypochlorous acid or free available chlorine, and 0.2 ppm of a copper salt, 0.2 ppm of a zinc salt and

0.2 ppm of an iron salt.

[193] In an embodiment, a composition disclosed herein comprises 0.01 ppm to 5 ppm hypochlorous

acid or free available chlorine, and 0.01 ppm to 2 ppm one or more metal salts. In an aspect of this

and 0.01 ppm to 0.5 ppm of a copper salt, 0,01 ppm to 0.5 ppm of a zinc salt, 0.01 ppm to 0.5 ppm of a

calcium salt, or any combination thereof. In an aspect of this embodiment, a composition disclosed herein

a copper salt, 0.05 ppm to 0.4 ppm of a zinc salt and 0.05 ppm to 0.4 ppm of a calcium salt. In an aspect

of this embodiment, a composition disclosed herein comprises 0.4 ppm to 0.6 ppm hypochlorous acid or

free available chlorine, and 0.1 ppm to 0.3 ppm of a copper salt, 0.1 ppm to 0.3 ppm of a zinc salt and 0.1

ppm to 0.3 ppm of a calcium salt. In an aspect of this embodiment, a composition disclosed herein

comprises 0.5 ppm hypochlorous acid or free available chlorine, and 0.2 ppm of a copper salt, 0.2 ppm of

a zinc salt and 0.2 ppm of a calcium salt.

[194] In an aspect of this embodiment, a composition disclosed herein comprises 0.01 ppm to 5 ppm

hypochlorous acid or free available chlorine, and 0.01 ppm to 1 ppm of a copper chloride, 0.01 ppm to 1

ppm of a zinc chloride, 0.01 ppm to 1 ppm of an iron chloride, or any combination thereof. In an aspect of

this embodiment, a composition disclosed herein comprises 0.05 ppm to 1 ppm hypochlorous acid or free

available chlorine, and 0.01 ppm to 0.5 ppm of a copper chloride, 0.01 ppm to 0.5 ppm of a zinc chloride,

0.01 ppm to 0.5 ppm of an iron chloride, or any combination thereof. In an aspect of this embodiment, a

composition disclosed herein comprises 0.05 ppm to 0.75 ppm hypochlorous acid or free available chlorine,

and 0.05 ppm to 0.4 ppm of a copper chloride, 0.05 ppm to 0.4 ppm of a zinc chloride and 0.05 ppm to 0.4

ppm of an iron chloride. In an aspect of this embodiment, a composition disclosed herein comprises 0.4

ppm to 0.6 ppm hypochlorous acid or free available chlorine, and 0.1 ppm to 0.3 ppm of a copper chloride,

0.1 ppm to 0.3 ppm of a zinc chloride and 0.1 ppm to 0.3 ppm of an iron chloride. In an aspect of this

embodiment, a composition disclosed herein comprises 0.5 ppm hypochlorous acid or free available

chlorine, and 0.2 ppm of a copper chloride, 0.2 ppm of a zinc chloride and 0.2 ppm of an iron chloride. The

iron chloride can be iron (II) chloride or iron (III) chloride.

[195] In an aspect of this embodiment, a composition disclosed herein comprises 0.01 ppm to 5 ppm

ppm of a zinc chloride, 0.01 ppm to 1 ppm of a calcium oxide salt, or any combination thereof. In an aspect

of this embodiment, a composition disclosed herein comprises 0.01 ppm to 1 ppm hypochlorous acid or

free available chlorine, and 0.01 ppm to 0.5 ppm of a copper chloride, 0.01 ppm to 0.5 ppm of a zinc

chloride, 0.01 ppm to 0.5 ppm of a calcium oxide salt, or any combination thereof. In an aspect of this

embodiment, a composition disclosed herein comprises 0.05 ppm to 0.75 ppm hypochlorous acid or free

available chlorine, and 0.05 ppm to 0.4 ppm of a copper chloride, 0.05 ppm to 0.4 ppm of a zinc chloride

WO wo 2020/252433 PCT/US2020/037665 88

and 0.05 ppm to 0.4 ppm of a calcium oxide salt. In an aspect of this embodiment, a composition disclosed

herein comprises 0.4 ppm to 0.6 ppm hypochlorous acid or free available chlorine, and 0.1 ppm to 0.3 ppm

of a copper chloride, 0.1 ppm to 0.3 ppm of a zinc chloride and 0.1 ppm to 0.3 ppm of a calcium oxide salt.

In an aspect of this embodiment, a composition disclosed herein comprises 0.5 ppm hypochlorous acid or

free available chlorine, and 0.2 ppm of a copper chloride, 0.2 ppm of a zinc chloride and 0.2 ppm of a

calcium oxide salt.

[196] In an embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm hypochlorous acid

or free available chlorine and 1 ppm to 100 ppm one or more metal particles. In an aspect of this

embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm hypochlorous acid or free

available chlorine and 1 ppm to 100 ppm of metal oxide particles. In an aspect of this embodiment, a

composition disclosed herein comprises 5 ppm to 75 ppm hypochlorous acid or free available chlorine and

5 ppm to 75 ppm of metal oxide particles. In an aspect of this embodiment, a composition disclosed herein

comprises 40 ppm to 60 ppm hypochlorous acid or free available chlorine, and 10 ppm to 30 ppm of metal

oxide particles. In an aspect of this embodiment, a composition disclosed herein comprises 50 ppm

hypochlorous acid or free available chlorine and 20 ppm of metal oxide particles.

[197] In an aspect of this embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm

hypochlorous acid or free available chlorine and 1 ppm to 100 ppm of copper zinc iron oxide particles. In

an aspect of this embodiment, a composition disclosed herein comprises 5 ppm to 75 ppm hypochlorous

acid or free available chlorine and 5 ppm to 75 ppm of copper zinc iron oxide particles. In an aspect of this

embodiment, a composition disclosed herein comprises 40 ppm to 60 ppm hypochlorous acid or free

available chlorine, and 10 ppm to 30 ppm of copper zinc iron oxide particles. In an aspect of this

embodiment, a composition disclosed herein comprises 50 ppm hypochlorous acid or free available

chlorine and 20 ppm of copper zinc iron oxide particles.

[198] In an embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm hypochlorous

acid or free available chlorine and 0.1 ppm to 10 ppm one or more metal particles. In an aspect of this

available chlorine and 0.1 ppm to 10 ppm of metal oxide particles. In an aspect of this embodiment, a

composition disclosed herein comprises 0.5 ppm to 7.5 ppm hypochlorous acid or free available chlorine

and 0.5 ppm to 7.5 ppm of metal oxide particles. In an aspect of this embodiment, a composition disclosed

herein comprises 4 ppm to 6 ppm hypochlorous acid or free available chlorine, and 1 ppm to 3 ppm of metal

oxide particles. In an aspect of this embodiment, a composition disclosed herein comprises 5 ppm

hypochlorous acid or free available chlorine and 2 ppm of metal oxide particles.

[199] In an aspect of this embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm

hypochlorous acid or free available chlorine and 0.1 ppm to 10 ppm of copper zinc iron oxide particles. In

an aspect of this embodiment, a composition disclosed herein comprises 0.5 ppm to 7.5 ppm hypochlorous

acid or free available chlorine and 0.5 ppm to 7.5 ppm of copper zinc iron oxide particles. In an aspect of

this embodiment, a composition disclosed herein comprises 4 ppm to 6 ppm hypochlorous acid or free

PCT/US2020/037665 89

available chlorine, and 1 ppm to 3 ppm of copper zinc iron oxide particles. In an aspect of this embodiment,

a composition disclosed herein comprises 5 ppm hypochlorous acid or free available chlorine and 2 ppm

of copper zinc iron oxide particles.

[200] In an embodiment, a composition disclosed herein comprises 0.01 ppm to 1 ppm hypochlorous

acid or free available chlorine and 0.01 ppm to 1 ppm one or more metal particles. In an aspect of this

embodiment, a composition disclosed herein comprises 0.01 ppm to 1 ppm hypochlorous acid or free

available chlorine and 0.01 ppm to 1 ppm of metal oxide particles. In an aspect of this embodiment, a

composition disclosed herein comprises 0.05 ppm to 0.75 ppm hypochlorous acid or free available chlorine

and 0.05 ppm to 0.75 ppm of metal oxide particles. In an aspect of this embodiment, a composition

disclosed herein comprises 0.4 ppm to 0.6 ppm hypochlorous acid or free available chlorine, and 0.1 ppm

to 0.3 ppm of metal oxide particles. In an aspect of this embodiment, a composition disclosed herein

comprises 0.5 ppm hypochlorous acid or free available chlorine and 0.2 ppm of metal oxide particles.

[201] In an aspect of this embodiment, a composition disclosed herein comprises 0.01 ppm to 1 ppm

hypochlorous acid or free available chlorine and 0.01 ppm to 1 ppm of copper zinc iron oxide particles. In

an aspect of this embodiment, a composition disclosed herein comprises 0.05 ppm to 0.75 ppm hypochlorous acid or free available chlorine and 0.05 ppm to 0.75 ppm of copper zinc iron oxide particles.

In an aspect of this embodiment, a composition disclosed herein comprises 0.4 ppm to 0.6 ppm

hypochlorous acid or free available chlorine, and 0.1 ppm to 0.3 ppm of copper zinc iron oxide particles. In

an aspect of this embodiment, a composition disclosed herein comprises 0.5 ppm hypochlorous acid or

free available chlorine and 0.2 ppm of copper zinc iron oxide particles.

[202] In an embodiment, a composition disclosed herein comprises about 0.1% to about 2% one or more

biguanide-containing compounds and about 1% to about 20% one or more alcohols. In an aspect of this

embodiment, a composition disclosed herein comprises about 0.25% to about 1.75% one or more biguanide-containing compounds and about 2% to about 15% one or more alcohols. In an aspect of this

embodiment, a composition disclosed herein comprises about 0.5% to about 1.5% one or more biguanide-

containing compounds and about 3% to about 12% one or more alcohols. In an aspect of this embodiment,

a composition disclosed herein comprises about 0.75% to about 1.25% one or more biguanide-containing

compounds and about 4% to about 10% one or more alcohols. In an aspect of this embodiment, a

composition disclosed herein comprises 1% one or more biguanide-containing compounds and about 5%

to about 7% one or more alcohols.

[203] In an aspect of this embodiment, a composition disclosed herein comprises about 0.25% to about

1.75% polyhexamethylene biguanide and about 2% to about 15% isopropanol. In an aspect of this

embodiment, a composition disclosed herein comprises about 0.5% to about 1.5% polyhexamethylene

biguanide and about 3% to about 12% isopropanol. In an aspect of this embodiment, a composition

disclosed herein comprises about 0.75% to about 1.25% polyhexamethylene biguanide and about 4% to

about 10% isopropanol. In an aspect of this embodiment, a composition disclosed herein comprises about

1% polyhexamethylene biguanide and about 5% to about 7% isopropanol.

PCT/US2020/037665 90

[204] In an embodiment, a composition disclosed herein comprises about 0.01% to about 0.2% one or

more biguanide-containing compounds and about 0.1% to about 2% one or more alcohols. In an aspect of

this embodiment, a composition disclosed herein comprises about 0.025% to about 0.175% one or more

biguanide-containing compounds and about 0.2% to about 1.5% one or more alcohols. In an aspect of this

embodiment, a composition disclosed herein comprises about 0.05% to about 0.15% one or more biguanide-containing compounds and about 0.3% to about 1.2% one or more alcohols. In an aspect of this

embodiment, a composition disclosed herein comprises about 0.075% to about 0.125% one or more

biguanide-containing compounds and about 0.4% to about 1% one or more alcohols. In an aspect of this

embodiment, a composition disclosed herein comprises 0.1% one or more biguanide-containing compounds and about 0.5% to about 0.7% one or more alcohols.

[205] In an aspect of this embodiment, a composition disclosed herein comprises about 0.025% to about

0.175% polyhexamethylene biguanide and about 0.2% to about 1.5% isopropanol. In an aspect of this

embodiment, a composition disclosed herein comprises about 0.05% to about 0.15% polyhexamethylene

biguanide and about 0.3% to about 1.2% isopropanol. In an aspect of this embodiment, a composition

disclosed herein comprises about 0.075% to about 0.125% polyhexamethylene biguanide and about 0.4%

to about 1% isopropanol. In an aspect of this embodiment, a composition disclosed herein comprises about

0.1% polyhexamethylene biguanide and about 0.5% to about 0.7% isopropanol.

[206] In an embodiment, a composition disclosed herein comprises about 0.001% to about 0.02% one

or more biguanide-containing compounds and about 0.01% to about 0.2% one or more alcohols. In an

aspect of this embodiment, a composition disclosed herein comprises about 0.0025% to about 0.0175%

one or more biguanide-containing compounds and about 0.02% to about 0.15% one or more alcohols. In

an aspect of this embodiment, a composition disclosed herein comprises about 0.005% to about 0.015%

one or more biguanide-containing compounds and about 0.03% to about 0.12% one or more alcohols. In

an aspect of this embodiment, a composition disclosed herein comprises about 0.0075% to about 0.0125%

one or more biguanide-containing compounds and about 0.04% to about 0.1% one or more alcohols. In

an aspect of this embodiment, a composition disclosed herein comprises 0.01% one or more biguanide-

containing compounds and about 0.05% to about 0.07% one or more alcohols.

[207] In an aspect of this embodiment, a composition disclosed herein comprises about 0.0025% to about

0.0175% polyhexamethylene biguanide and about 0.02% to about 0.15% isopropanol. In an aspect of this

embodiment, a composition disclosed herein comprises about 0.005% to about 0.015% polyhexamethylene

biguanide and about 0.03% to about 0.12% isopropanol. In an aspect of this embodiment, a composition

disclosed herein comprises about 0.0075% to about 0.0125% polyhexamethylene biguanide and about

0.04% to about 0.1% isopropanol. In an aspect of this embodiment, a composition disclosed herein

comprises about 0.01% polyhexamethylene biguanide and about 0.05% to about 0.07% isopropanol.

[208] In an embodiment, a composition disclosed herein comprises about 0.1% to about 2% one or more

biguanide-containing compounds, about 1% to about 20% one or more alcohols, and about 0.1% to about

PCT/US2020/037665 91 91

2% of one or more quaternary ammonium salts. In an aspect of this embodiment, a composition disclosed

herein comprises about 0.25% to about 1.75% one or more biguanide-containing compounds, about 2% to

about 15% one or more alcohols, and about 0.2% to about 1.5% of one or more quaternary ammonium

salts. In an aspect of this embodiment, a composition disclosed herein comprises about 0.5% to about

1.5% one or more biguanide-containing compounds, about 3% to about 12% one or more alcohols, and

about 0.3% to about 1% of one or more quaternary ammonium salts. In an aspect of this embodiment, a

composition disclosed herein comprises about 0.75% to about 1.25% one or more biguanide-containing

compounds, about 4% to about 10% one or more alcohols, and about 0.4% to about 0.6% of one or more

quaternary ammonium salts. In an aspect of this embodiment, a composition disclosed herein comprises

1% one or more biguanide-containing compounds, about 5% to about 7% one or more alcohols, and about

0.5% one or more quaternary ammonium salts.

[209] In an embodiment, a composition disclosed herein comprises about 0.1% to about 2% polyhexamethylene biguanide, about 1% to about 20% isopropanol, and about 0.1% to about 2% of one or

more cetylpyridinium salts. In an aspect of this embodiment, a composition disclosed herein comprises

about 0,25% to about 1.75% polyhexamethylene biguanide, about 2% to about 15% isopropanol, and about

0.2% to about 1.5% of one or more cetylpyridinium salts. In an aspect of this embodiment, a composition

disclosed herein comprises about 0.5% to about 1.5% polyhexamethylene biguanide, about 3% to about

12% isopropanol, and about 0.3% to about 1% of one or more cetylpyridinium salts. In an aspect of this

embodiment, a composition disclosed herein comprises about 0.75% to about 1.25% polyhexamethylene

biguanide, about 4% to about 10% isopropanol, and about 0.4% to about 0.6% of one or more cetylpyridinium salts. In an aspect of this embodiment, a composition disclosed herein comprises 1%

polyhexamethylene biguanide, about 5% to about 7% isopropanol, and about 0.5% one or more cetylpyridinium salts.

[210] In an embodiment, a composition disclosed herein comprises about 0.01% to about 0.2% one or

more biguanide-containing compounds, about 0.1% to about 2% one or more alcohols, and about 0.01%

to about 0.2% of one or more quaternary ammonium salts. In an aspect of this embodiment, a composition

disclosed herein comprises about 0.025% to about 0.175% one or more biguanide-containing compounds,

about 0.2% to about 1.5% one or more alcohols, and about 0.02% to about 0.15% of one or more quaternary

ammonium salts. In an aspect of this embodiment, a composition disclosed herein comprises about 0.05%

to about 0.15% one or more biguanide-containing compounds, about 0.3% to about 1.2% one or more

alcohols, and about 0.03% to about 0.1% of one or more quaternary ammonium salts. In an aspect of this

biguanide-containing compounds, about 0.4% to about 1% one or more alcohols, and about 0.04% to about

0.06% of one or more quaternary ammonium salts. In an aspect of this embodiment, a composition

disclosed herein comprises 0.1% one or more biguanide-containing compounds, about 0.5% to about 0.7%

one or more alcohols, and about 0.05% one or more quaternary ammonium salts.

[211] In an embodiment, a composition disclosed herein comprises about 0.01% to about 0.2% polyhexamethylene biguanide, about 0.1% to about 2% isopropanol, and about 0.01% to about 0.2% of one or more cetylpyridinium salts. In an aspect of this embodiment, a composition disclosed herein comprises about 0.025% to about 0.175% polyhexamethylene biguanide, about 0.2% to about 1.5% isopropanol, and about 0.02% to about 0.15% of one or more cetylpyridinium salts. In an aspect of this embodiment, a composition disclosed herein comprises about 0.05% to about 0.15% polyhexamethylene biguanide, about 0.3% to about 1.2% isopropanol, and about 0.03% to about 0.1% of one or more cetylpyridinium salts. In an aspect of this embodiment, a composition disclosed herein comprises about

0.075% to about 0.125% polyhexamethylene biguanide, about 0.4% to about 1% isopropanol, and about

0.04% to about 0.06% of one or more cetylpyridinium salts. In an aspect of this embodiment, a composition

disclosed herein comprises 0.1% polyhexamethylene biguanide, about 0.5% to about 0.7% isopropanol,

and about 0.05% one or more cetylpyridinium salts.

[212] In an embodiment, a composition disclosed herein comprises about 0.001% to about 0.02% one

or more biguanide-containing compounds, about 0.01% to about 0.2% one or more alcohols, and about

0.001% to about 0.02% of one or more quaternary ammonium salts. In an aspect of this embodiment, a

composition disclosed herein comprises about 0.0025% to about 0.0175% one or more biguanide-

containing compounds, about 0.02% to about 0.15% one or more alcohols, and about 0.002% to about

0.015% of one or more quaternary ammonium salts. In an aspect of this embodiment, a composition

disclosed herein comprises about 0.005% to about 0.015% one or more biguanide-containing compounds,

about 0.03% to about 0.12% one or more alcohols, and about 0.003% to about 0.01% of one or more

about 0.0075% to about 0.0125% one or more biguanide-containing compounds, about 0.04% to about

0.1% one or more alcohols, and about 0.004% to about 0.006% of one or more quaternary ammonium

salts. In an aspect of this embodiment, a composition disclosed herein comprises 0.01% one or more

biguanide-containing compounds, about 0.05% to about 0.07% one or more alcohols, and about 0.005%

one or more quaternary ammonium salts.

[213] In an embodiment, a composition disclosed herein comprises about 0.001% to about 0.02%

polyhexamethylene biguanide, about 0.01% to about 0.2% isopropanol, and about 0.001% to about 0.02%

of one or more cetylpyridinium salts. In an aspect of this embodiment, a composition disclosed herein

comprises about 0.0025% to about 0.0175% polyhexamethylene biguanide, about 0.02% to about 0.15%

isopropanol, and about 0.002% to about 0.015% of one or more cetylpyridinium salts. In an aspect of this

biguanide, about 0.03% to about 0.12% isopropanol, and about 0.003% to about 0.01% of one or more

cetylpyridinium salts. In an aspect of this embodiment, a composition disclosed herein comprises about

0.0075% to about 0.0125% polyhexamethylene biguanide, about 0.04% to about 0.1% isopropanol, and

about 0.004% to about 0.006% of one or more cetylpyridinium salts. In an aspect of this embodiment, a

composition disclosed herein comprises 0.01% polyhexamethylene biguanide, about 0.05% to about 0.07%

isopropanol, and about 0.005% one or more cetylpyridinium salts.

[214] In an embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm hypochlorous acid

or free available chlorine and 1 ppm to 100 ppm one or more quaternary compounds or silicone quaternary

WO wo 2020/252433 PCT/US2020/037665 93

compounds. In an aspect of this embodiment, a composition disclosed herein comprises 1 ppm to 100

ppm hypochlorous acid or free available chlorine and 1 ppm to 100 ppm of one or more silicone dialkyl

quaternary compounds. In an aspect of this embodiment, a composition disclosed herein comprises 5 ppm

to 75 ppm hypochlorous acid or free available chlorine and 5 ppm to 75 ppm of one or more silicone dialkyl

quaternary compounds. In an aspect of this embodiment, a composition disclosed herein comprises 40

ppm to 60 ppm hypochlorous acid or free available chlorine, and 40 ppm to 60 ppm of one or more silicone

dialkyl quaternary compounds. In an aspect of this embodiment, a composition disclosed herein comprises

50 ppm hypochlorous acid or free available chlorine and 50 ppm of one or more silicone dialkyl quaternary

compounds.

[215] In an aspect of this embodiment, a composition disclosed herein comprises 1 ppm to 100 ppm

hypochlorous acid or free available chlorine and 1 ppm to 100 ppm of dimethyloctadecyl[3- (trimethoxysilyl)propyl]ammonium chloride. In an aspect of this embodiment, a composition disclosed

herein comprises 5 ppm to 75 ppm hypochlorous acid or free available chlorine and 5 ppm to 75 ppm of

dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride. In an aspect of this embodiment, a

and 40 ppm to 60 ppm of dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride. In an aspect of

this embodiment, a composition disclosed herein comprises 50 ppm hypochlorous acid or free available

chlorine and 50 ppm of dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride.

[216] In an embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm hypochlorous

available chlorine and 0.1 ppm to 10 ppm of one or more silicone dialkyl quaternary compounds. In an

aspect of this embodiment, a composition disclosed herein comprises 0.5 ppm to 7.5 ppm hypochlorous

acid or free available chlorine and 0.5 ppm to 7.5 ppm of one or more silicone dialkyl quaternary

compounds. In an aspect of this embodiment, a composition disclosed herein comprises 4 ppm to 6 ppm

hypochlorous acid or free available chlorine, and 4 ppm to 6 ppm of one or more silicone dialkyl quaternary

compounds. In an aspect of this embodiment, a composition disclosed herein comprises 5 ppm hypochlorous acid or free available chlorine and 5 ppm of one or more silicone dialkyl quaternary

compounds.

[217] In an aspect of this embodiment, a composition disclosed herein comprises 0.1 ppm to 10 ppm

hypochlorous acid or free available chlorine and 0.1 ppm to 10 ppm of dimethyloctadecyl[3- (trimethoxysilyl)propyl]ammonium chloride. In an aspect of this embodiment, a composition disclosed

herein comprises 0.5 ppm to 7.5 ppm hypochlorous acid or free available chlorine and 0.5 ppm to 7.5 ppm

of dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium o chloride. In an aspect of this embodiment, a

composition disclosed herein comprises 4 ppm to 6 ppm hypochlorous acid or free available chlorine, and

4 ppm to 6 ppm of dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride. In an aspect of this

embodiment, a composition disclosed herein comprises 5 ppm hypochlorous acid or free available chlorine

and 5 ppm of dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride.

[218] In an embodiment, a composition disclosed herein comprises 0.01 ppm to 1 ppm hypochlorous

available chlorine and 0.01 ppm to 1 ppm of one or more silicone dialkyl quaternary compounds. In an

aspect of this embodiment, a composition disclosed herein comprises 0.05 ppm to 0.75 ppm hypochlorous

acid or free available chlorine and 0.05 ppm to 0.75 ppm of one or more silicone dialkyl quaternary

compounds. In an aspect of this embodiment, a composition disclosed herein comprises 0.4 ppm to 0.6

ppm hypochlorous acid or free available chlorine, and 0.4 ppm to 0.6 ppm of one or more silicone dialkyl

quaternary compounds. In an aspect of this embodiment, a composition disclosed herein comprises 0.5

ppm hypochlorous acid or free available chlorine and 0.5 ppm of one or more silicone dialkyl quaternary

compounds.

[219] In an aspect of this embodiment, a composition disclosed herein comprises 0.01 ppm to 1 ppm

hypochlorous acid or free available chlorine and 0.01 ppm to 1 ppm of dimethyloctadecyl[3- (trimethoxysilyl)propyl]ammonium chloride. In an aspect of this embodiment, a composition disclosed

herein comprises 0.05 ppm to 0.75 ppm hypochlorous acid or free available chlorine and 0.05 ppm to 0.75

ppm of dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride. In an aspect of this embodiment,

a composition disclosed herein comprises 0.4 ppm to 0.6 ppm hypochlorous acid or free available chlorine,

and 0.4 ppm to 0.6 ppm ofdimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride. In an aspect of

this embodiment, a composition disclosed herein comprises 0.5 ppm hypochlorous acid or free available

chlorine and 0.5 ppm of dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammoniumchloride.

[220] Aspects of the present specification disclose a kit. In one embodiment, the kit can comprise a

container including a composition disclosed herein. In one embodiment, the kit can comprise a container

including a microbiostatic agent disclosed herein. In another embodiment, a kit can comprise one or more

containers, each container including an individual component like a microbiostatic agent disclosed herein

or more than one individual components like more than one microbiostatic agents disclosed herein in

combination. For example, a kit can comprise one container including hypochlorous acid or free available

chlorine and a second container including one or more metallic particles. As another example, a kit can

comprise one container including hypochlorous acid or free available chlorine and a second container

including copper zinc iron oxide particles. As yet another example, a kit can comprise one container

including one or more alcohols and a second container including one or more guanide-containing

compounds. As still another example, a kit can comprise one container including isopropanol and a second

container including polyhexamethylene biguanide. As a further example, a kit can comprise one container

including hypochlorous acid or free available chlorine and a second container including one or more

quaternary compounds or silicon quaternary compounds. As another example, a kit can comprise one

container including hypochlorous acid or free available chlorine and a second container including

dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammoniumo chloride. The remainder of the components of a

composition disclosed herein may be included in either the first or second container, or may be separately

including in at least a third container. For example, a third container including a rinse solution disclosed

PCT/US2020/037665 95

herein can be included in the kit. Packaging of individual components into separate container can assist in

prolonging the stability of the individual components, and thus shelf life of the product.

[221] In another example, a kit can comprise one container including hypochlorous acid and/or free

available chlorine, a second container including one or more metallic particles, and a third container

including a rinse solution. As another example, a kit can comprise one container including hypochlorous

acid and/or free available chlorine, a second container including copper zinc iron oxide particles, and a third

container including a rinse solution. As yet another example, a kit can comprise one container including

one or more alcohols, a second container including one or more guanide-containing compounds, and a

third container including a rinse solution. As still another example, a kit can comprise one container

including isopropanol, a second container including polyhexamethylene biguanide, and a third container

including a rinse solution. As a further example, a kit can comprise one container including hypochlorous

acid or free available chlorine, a second container including one or more quaternary compounds or silicon

quaternary compounds, and a third container including a rinse solution. As another example, a kit can

comprise one container including hypochlorous acid or free available chlorine, a second container including

dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride, and a third container including a rinse

solution. As another example, a kit can comprise one container including isopropanol, a second container

including polyhexamethylene biguanide, a third container including one or more cationic surfactants, and a

fourth container including a rinse solution.

[222] In another embodiment, a kit can comprise one container including a composition disclosed herein

and a second container including a rinse solution disclosed herein. In another embodiment, a kit can

comprise one container including a microbiostatic agent disclosed herein and a second container including

a rinse solution disclosed herein. For example, a kit can comprise one container including hypochlorous

acid and/or free available chlorine and one or more metallic particles and a second container including a

rinse solution. As another example, a kit can comprise one container including hypochlorous acid and/or

free available chlorine and copper zinc iron oxide particles and a second container including a rinse solution.

As yet another example, a kit can comprise one container including one or more alcohols and one or more

guanide-containing compounds and a second container including a rinse solution. As still another example,

a kit can comprise one container including isopropanol and polyhexamethylene biguanide and a second

container including a rinse solution. As a further example, a kit can comprise one container including

hypochlorous acid and/or free available chlorine and one or more quaternary compounds or silicon

quaternary compounds and a second container including a rinse solution. As another example, a kit can

comprise one container including hypochlorous acid and/or free available chlorine and dimethyloctadecyl[3

(trimethoxysilyl)propyl]ammonium chloride and a second container including a rinse solution. The

remainder of the components of a composition disclosed herein may be included in either the first container

or may be separately including in at least a third container. Packaging of individual components into

separate containers can assist in prolonging the stability of the individual components, and thus shelf life

of the product.

[223] A kit disclosed herein can comprise a delivery or application system. The delivery or application

system of the kit are useful for applying a composition disclosed herein, and/or individual components

disclosed herein to a site of interest, such as, e.g., a surface of a device disclosed herein. A delivery or

application system disclosed herein, includes, without limitation, one or more of an applicator brush, porous

foam swab or pad, hollow tube, dipstick, or a combination thereof. In an embodiment, a kit comprises a

single delivery or application system. In another embodiment, a kit comprises a plurality delivery or

application systems. For example, in a 30-day supply kit, there can be 30 delivery or application systems,

such that there is one delivery or application system per day for 30 days. Alternately, there can be 2, 10,

20, 30, 40, 50, 60, 90, 120, etc. delivery or application systems per kit. Within the kit, the delivery or

application system may be packaged individually, or in sets of 2 or more. The delivery or application system

can be packaged such that it remains sterile until use. In certain embodiments, a delivery or application

system disclosed herein can be packaged in plastic sheaths. Further, to prevent contamination, delivery

or application system disclosed herein is preferably single-use, disposable delivery or application system.

[224] The kit can also comprise a set of instructions. The instructions may include information useful to

the end user such as how to use a delivery or application system to apply a composition and/or individual

components disclosed herein, and/or how often to apply a composition and/or individual components

disclosed herein. In addition, such instructions may include information regarding how to mix the individual

components disclosed herein to form a composition disclosed herein. Such instructions can indicate that

mixing should be done at a certain time before application, such as, e.g., just prior to use. Instructions

disclosed herein may also include information regarding how to apply the individual components disclosed

herein directly to a site of interest, such as, e.g., a surface of a device disclosed herein, and in what order

the individual components should be applied to such sites of interest.

[225] The contents of the kit, including the container including a composition, a microbiostatic agent, or

other component disclosed herein, the delivery or application system, and instructions, are enclosed in an

outer casing. The outer casing can be a box, a sealed bag, a foil pouch, etc. In certain embodiments, the

delivery system, container and instructions are enclosed in a box. In other embodiments of the kit, the

container and instructions are contained in a first box, the delivery system is contained in a second box,

and the first and second box are contained together in a third box.

[226] Although water does not provide an environment favorable for microbial growth, water can become

subsequently contaminated with organic matter that can then support microbial growth in the water. As

such, the presently disclosed compositions are useful in any application involving the restraining or

inhibition of microbial growth in water or other liquid exposed to microbes or organic matter which supports

microbial growth. In one embodiment, a composition disclosed herein is used to reduce or inhibit microbial

growth in water or other liquid present in a device during use or storage of the device. The water or other

liquid can be manufactured as sterile or non-sterile. In one embodiment, a composition disclosed herein is

used to reduce or inhibit microbial growth in water or other liquid present in a medical device during use or

storage of the medical device. In one embodiment, a composition disclosed herein is used to reduce or

inhibit microbial growth in water or other liquid present in a non-medical device during use or storage of the non-medical device. In one embodiment, a composition disclosed herein is used to reduce or inhibit microbial growth in water or other liquid present in a residential, commercial or industrial device during use or storage of the residential, commercial or industrial device.

[227] In one embodiment, a composition disclosed herein is used to reduce or inhibit microbial growth in

water or other liquid. In one embodiment, a composition disclosed herein is used to reduce or inhibit

microbial growth in water or other liquid present in a water reservoir or other component of a device. In one

embodiment, a composition disclosed herein is used to reduce or inhibit microbial growth in water or other

liquid present in a water reservoir or other component of a medical device. In one embodiment, a

composition disclosed herein is used to reduce or inhibit microbial growth in water or other liquid present in

a water reservoir or other component of a non-medical device. In one embodiment, a composition disclosed

herein is used to reduce or inhibit microbial growth in water or other liquid present in a water reservoir or

other component of a residential, commercial or industrial device.

[228] In one embodiment, a composition disclosed herein is used to wash or irrigate a surface area. In

one embodiment, a composition disclosed herein is used to wash or irrigate a biological surface, a tissue,

a skin, or a body cavity.

[229] In one embodiment, a composition disclosed herein is used to wash or irrigate a wound in an

individual. In one embodiment, a composition disclosed herein is used to wash or irrigate an infection in

an individual. In one embodiment, a composition disclosed herein is used to treat and/or provide relief from

a wound or infection in an individual.

[230] Aspects of the present specification disclose a making a microbiostatic water or other liquid. Such

methods are designed to restrain, prohibit, or otherwise prevents population growth of microbes pre-existing

in water or other liquid or subsequently introduced to the water or other liquid. In aspects of this

embodiment, a method disclosed herein comprises applying one or more microbiostatic agents disclosed

herein to water or other liquid in a microbiostatic effective amount. In other aspects of this embodiment, a

method disclosed herein comprises applying one or more microbiostatic agents disclosed herein to water

or other liquid in an amount that restrains, prohibits, or otherwise prevents microbial population growth

without eradicating the microbes. In yet other aspects of this embodiment, a method disclosed herein

comprises applying one or more microbiostatic agents disclosed herein to water or other liquid in an amount

to a degree that achieves disinfection or sterilization.

[231] Aspects of the present specification disclose a method of reducing or inhibiting microbial growth in

water or other liquid. Such methods are designed to restrain, prohibit, or otherwise prevent population

growth of microbes pre-existing in water or other liquid or subsequently introduced to the water or other

liquid. In aspects of this embodiment, a method disclosed herein comprises applying a composition

disclosed herein to water or other liquid in a microbiostatic effective amount, wherein application of the

composition reduces or inhibits microbial growth. In other aspects of this embodiment, a method disclosed herein comprises applying a composition disclosed herein to water or other liquid in an amount that restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbes, wherein application of the composition reduces or inhibits microbial growth. In yet other aspects of this embodiment, a method disclosed herein comprises applying a composition disclosed herein to water or other liquid in an amount that restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbes to a degree that achieves disinfection or sterilization, wherein application of the composition reduces or inhibits microbial growth.

[232] In aspects of this embodiment, a composition disclosed herein is for use in reducing or inhibiting

microbial growth in water or other liquid. In other aspects of this embodiment, a use of a disclosed

composition is to reduce or inhibit microbial growth in water or other liquid. In yet other aspects of this

embodiment, a use of a composition disclosed herein is to manufacture a medicament of water or other

liquid to reduce or inhibit microbial growth.

[233] Aspects of the present specification disclose a method of reducing or inhibiting microbial growth in

a medical device. Such methods are designed to restrain, prohibit, or otherwise prevent population growth

of microbes present in a medical device, prevent the spread of such microbial contamination to other areas

or components of the medical device as well as reduce or inhibit microbial growth of microbes subsequently

introduced to the medical device.

[234] In an embodiment, a method disclosed herein applies a composition disclosed herein or one or

more microbiostatic agents disclosed herein directly to water or other liquid. In this embodiment,

composition disclosed herein or one or more microbiostatic agents disclosed herein is used as a

concentrate that is diluted in water or other liquid.

[235] In aspects of this embodiment, a method disclosed herein applies a composition disclosed herein

to water or other liquid already present in a medical device, or a component of a medical device, such as,

e,g,, a reservoir or other storage container for water or other liquid. A method disclosed herein can also

apply a composition disclosed herein to water or other liquid prior to introduction to a medical device, and

then adding this admixture to a medical device, or a component of a medical device, such as, e,g,, a

reservoir or other storage container for water or other liquid. In aspects of this embodiment, a disclosed

method comprises applying a composition disclosed herein to water or other liquid present in or to be added

toa medical device in a microbiostatic effective amount, wherein application of the composition reduces or

inhibits microbial growth in the water or other liquid and/or in the medical device. In other aspects of this

embodiment, a disclosed method comprises applying a composition disclosed herein to water or other liquid

present in or to be added to a medical device in an amount that restrains, prohibits, or otherwise prevents

microbial population growth without eradicating the microbes, wherein application of the composition

reduces or inhibits microbial growth in the water or other liquid and/or in the medical device. In yet other

aspects of this embodiment, a disclosed method comprises applying a composition disclosed herein to

water or other liquid present in or to be added to a medical device in an amount that restrains, prohibits, or

otherwise prevents microbial population growth without eradicating the microbes to a degree that achieves

WO wo 2020/252433 PCT/US2020/037665 PCT/US2020/037665 99

disinfection or sterilization, wherein application of the composition reduces or inhibits microbial growth in

the water or other liquid and/or in the medical device.

[236] In aspects of this embodiment, a disclosed method comprises applying one or more microbiostatic

agents disclosed herein to water or other liquid present in or to be added to a medical device in a

microbiostatic effective amount, wherein application of the one or more microbiostatic agents reduces or

embodiment, a disclosed method comprises applying one or more microbiostatic agents disclosed herein

to water or other liquid present in or to be added to a medical device in an amount that restrains, prohibits,

or otherwise prevents microbial population growth without eradicating the microbes, wherein application of

the one or more microbiostatic agents reduces or inhibits microbial growth in the water or other liquid and/or

in the medical device. In yet other aspects of this embodiment, a disclosed method comprises applying

one or more microbiostatic agents disclosed herein to water or other liquid present in or to be added to a

medical device in an amount that restrains, prohibits, or otherwise prevents microbial population growth

without eradicating the microbes to a degree that achieves disinfection or sterilization, wherein application

of the one or more microbiostatic agents reduces or inhibits microbial growth in the water or other liquid

and/or in the medical device.

[237] In another embodiment, a method disclosed herein applies a composition disclosed herein in an

undiluted which is added directly to a medical device, or a component of a medical device, such as, e,g,, a

reservoir or other storage container for water or other liquid. In this embodiment, a composition disclosed

herein is used as a substitute or replacement for water or other liquid and would typically be used in an

undiluted form. In aspects of this embodiment, a disclosed method comprises applying a composition

disclosed herein directly to a medical device, or a component of a medical device, such as, e,g,, a reservoir

or other storage container for water or other liquid, wherein application of the composition reduces or inhibits

microbial growth prior and during operation of the device.

[238] In aspects of this embodiment, a composition disclosed herein is for use in reducing or inhibiting

microbial growth in a medical device. In other aspects of this embodiment, a use of a composition disclosed

herein is to reduce or inhibit microbial growth in a medical device. In yet other aspects of this embodiment,

a use of a composition disclosed herein is to manufacture a medicament to reduce or inhibit microbial

growth in a medical device.

[239] Aspects of the present specification disclose a method of reducing or inhibiting microbial growth in

a residential, commercial or industrial device. Such methods are designed to restrain, prohibit, or otherwise

prevent population growth of microbes present in a residential, commercial or industrial device, prevent

the spread of such microbial contamination to other areas or components of the residential, commercial or

industrial device as well as restrain, prohibit, or otherwise prevent population growth of microbes

subsequently introduced to the residential, commercial or industrial device.

PCT/US2020/037665 100

[240] In an embodiment, a method disclosed herein applies a composition disclosed herein or one or

concentrate that is diluted in water or other liquid.

[241] In aspects of this embodiment, a method disclosed herein applies a composition disclosed herein

to water or other liquid already present in a residential, commercial or industrial device, or a component of

a residential, commercial or industrial device, such as, e,g,, a reservoir or other storage container for water

or other liquid. A method disclosed herein can also apply a composition disclosed herein to water or other

liquid prior to introduction to a residential, commercial or industrial device, and then adding this admixture

to a residential, commercial or industrial device, or a component of a residential, commercial or industrial

device, such as, e,g,, a reservoir or other storage container for water or other liquid. In aspects of this

embodiment, a method disclosed herein comprises applying a composition disclosed herein to water or

other liquid present in or to be added to a residential, commercial or industrial device in a microbiostatic

effective amount, wherein application of the composition reduces or inhibits microbial growth in the water

or other liquid and/or in the residential, commercial or industrial device. In other aspects of this

present in or to be added to a residential, commercial or industrial device in an amount that restrains,

prohibits, or otherwise prevents microbial population growth without eradicating the microbes, wherein

application of the composition reduces or inhibits microbial growth in the water or other liquid and/or in the

residential, commercial or industrial device. In yet other aspects of this embodiment, a disclosed method

comprises applying a composition disclosed herein to water or other liquid present in or to be added to a

residential, commercial or industrial device in an amount that restrains, prohibits, or otherwise prevents

sterilization, wherein application of the composition reduces or inhibits microbial growth in the water or other

liquid and/or in the residential, commercial or industrial device.

[242] In aspects of this embodiment, a disclosed method comprises applying one or more microbiostatic

agents disclosed herein to water or other liquid present in or to be added to a residential, commercial or

industrial device in a microbiostatic effective amount, wherein application of the one or more microbiostatic

agents reduces or inhibits microbial growth in the water or other liquid and/or in the residential, commercial

or industrial device. In other aspects of this embodiment, a disclosed method comprises applying one or

more microbiostatic agents disclosed herein to water or other liquid present in or to be added to a

microbial population growth without eradicating the microbes, wherein application of the one or more

microbiostatic agents reduces or inhibits microbial growth in the water or other liquid and/or in the

comprises applying one or more microbiostatic agents disclosed herein to water or other liquid present in

or to be added to a residential, commercial or industrial device in an amount that restrains, prohibits, or

WO wo 2020/252433 PCT/US2020/037665 101

disinfection or sterilization, wherein application of the one or more microbiostatic agents reduces or inhibits

microbial growth in the water or other liquid and/or in the residential, commercial or industrial device.

[243] In another embodiment, a method disclosed herein applies a composition disclosed herein in an

undiluted which is added directly to a residential, commercial or industrial device, or a component of a

residential, commercial or industrial device, such as, e,g,, a reservoir or other storage container for water

or other liquid. In this embodiment, a composition disclosed herein is used as a substitute or replacement

for water or other liquid and would typically be used in an undiluted form. In other aspects, a disclosed

method comprises applying a composition disclosed herein directly to a residential, commercial or industrial

operation of the device.

[244] In aspects of this embodiment, a composition disclosed herein is for use in reducing or inhibiting

microbial growth in a residential, commercial or industrial device. In other aspects of this embodiment, a

use of a composition disclosed herein is to reduce or inhibit microbial growth in a residential, commercial

or industrial device. In yet other aspects of this embodiment, a use of a composition disclosed herein is to

manufacture a medicament to reduce or inhibit microbial growth in a residential, commercial or industrial

device.

[245] In an embodiment, a method disclosed herein does not disinfect or sterilize water or other liquid.

Both sterilization and disinfection kill or otherwise eradicate microbes. Sterilization is a process that

destroys or eliminates all forms of a microbe including microbial spores. Water or other liquid disclosed

herein is considered to be sterilized when there is a X 106 log reduction of microbes. Disinfection destroys

or eliminates most recognized pathogenic microbes but not necessarily all microbial forms (e.g., microbial

spores). Water or other liquid disclosed herein is considered to be sterilized when there is a X 104

reduction of microbes. Since the amount of a composition disclosed herein is used in a microbiostatic

effective amount in a method disclosed herein, the method does not sterilize or disinfect the water or other

liquid. In aspects of this embodiment, treating a water or other liquid with a composition disclosed herein

or one or more microbiostatic agents disclosed herein does not result in a 1 X 104 or greater reduction of

microbes. In other aspects of this embodiment, treating a water or other liquid with a composition disclosed

herein or one or more microbiostatic agents disclosed herein does not result in a 1 X 105 or greater reduction

of microbes. In yet other aspects of this embodiment, treating a water or other liquid with a composition

disclosed herein or one or more microbiostatic agents disclosed herein does not result in a 1 X 106 or greater

reduction of microbes.

[246] In aspects of this embodiment, treating a water or other liquid with a composition disclosed herein

or one or more microbiostatic agents disclosed herein results in, e.g., about 1 X 10° reduction in microbes,

about 1 X 10 1 reduction in microbes, about 1 X 102 reduction in microbes, or about 1 X 103 reduction in

microbes, In other aspects of this embodiment, treating a water or other liquid with a composition disclosed

herein or one or more microbiostatic agents disclosed herein results in, e.g., at most 1 X 10° reduction in

microbes, at most 1 X 101 reduction in microbes, at most 1 X 102 reduction in microbes, or at most 1 X 10³ reduction in microbes, In yet other aspects of this embodiment, treating a water or other liquid with a composition disclosed herein or one or more microbiostatic agents disclosed herein results in, e.g., about

1 X 10° to about X 10 ¹ reduction in microbes, about 1 X 10° to about X 102 reduction in microbes, about

1 X 10° to about 1 X 10³ reduction in microbes, about 1 X 101 to about 1 X 102 reduction in microbes, about

1 X 101 to about X 10³ reduction in microbes, or about 1 X 102 to about 1 X 103 reduction in microbes.

[247] The term "other liquid" refers to any single or multi-phase fluid that can support or is susceptible to

microbial contamination and growth. In aspects of this embodiment, a liquid or "other liquid" is single or

multi-phase liquid comprising water. A liquid or "other liquid" can be a pharmaceutical carrier, such as, e.g.,

vehicle, stabilizer, diluent, additive, auxiliary, or excipient, used to formulate a pharmaceutical drug or

prepare such a drug for administration to an individual. A liquid or "other liquid" can be a buffered solution,

a medical solution, a contact solution, a dental solution, a mouthwash solution, or a denture solution. A

liquid or "other liquid" can be a fluid used as a coolant, a flushing agent, a washing agent or other fluid

susceptible to microbial contamination when stored or used in devices, equipment, appliances, machinery,

or other apparatus.

[248] A medical device is an instrument, apparatus, material, or other article, whether used alone or in

combination, including software necessary for its application, intended by the manufacturer to be used for

human beings for diagnosis, prevention, monitoring treatment, or alleviation of disease; diagnosis,

monitoring, treatment, or alleviation of or compensation for an injury or handicap; investigation,

replacement, or modification of the anatomy or of a physiologic process; or control of conception, and that

does not achieve its primary intended action in or on the human body by pharmacologic, immunologic, or

metabolic means but might be assisted in its function by such means. A medical device includes, without

limitation, a surgical instrument, a dental instrument, a respiratory therapy instrument including positive-

pressure ventilators and negative-pressure ventilators, an anesthesia instrument, a catheter, an implant, a

probe, an endoscope, an arthroscope, a laparoscope, a blade, a cystoscope, a spirometer, a continuous

positive airway pressure (CPAP) mask and tubing, a positive end-expiratory pressure (PEEP) mask and

tubing, dialysis instrument and accessories, a heart-lung machine and accessories, a heart-lung bypass

machine and accessories, and a diaphragm fitting ring. Non-limiting examples of a probe includes an

ultrasound probe and an esophageal manometry probe. Non-limiting examples of a catheter includes a

cardiac catheter, an urinary catheter, an anorectal manometry catheter. Non-limiting examples of an

endoscope includes a gastrointestinal endoscope, a bronchoscope, and a nasopharyngoscope. Non-

limiting examples of a blade includes a laryngoscope blade.

[249] Aspects of the present specification disclose a method to wash or irrigate a surface area. Such

methods are designed to prevent introduction of microbes to a surface area due to adverse levels of

contamination in a composition disclosed herein. In aspects of this embodiment, a method disclosed herein

comprises applying a composition disclosed herein to a surface area, wherein application of the

composition washes or irrigates the surface area.

PCT/US2020/037665 103

[250] In other aspects of this embodiment, a composition disclosed herein is for use in washing or

irrigating a surface area. In other aspects of this embodiment, a use of a disclosed composition is to wash

or irrigate a surface area. A surface area can be a porous surface area or a non-porous surface area

including a skin surface of an individual or a hard surface of a medical or a non-medical device, equipment,

appliance, machinery, or other apparatus.

[251] In an embodiment, a method disclosed herein does not disinfect or sterilize a surface area.

[252] In another aspect of this embodiment, a method disclosed herein comprises applying one or more

individual components disclosed herein to a hard surface area for a specified amount of time, wherein

application results in the washing or irrigation of the hard surface area. In an aspect of this embodiment,

the one or more individual components include a first component including hypochlorous acid or free

available chlorine and a second component including one or more disinfectants. In another aspect,

application of the one or more individual components occur in a specific order, such as. e.g., first applying

a first component including hypochlorous acid or free available chlorine and then applying a second

component including one or more disinfectants. A hard surface area can be a porous surface area or a

non-porous surface area.

[253] A hard surface area can include any items present in a residence or a commercial, industrial and/or

agricultural facility, such as, e.g., a hospital, a laboratory, a restaurant, an educational center, a food-

processing facility, a dairy-processing facility, an airport, an oil field system, a sport facility, a shipping dock,

a freight transport center, or any other commercial or industrial setting. A surface area can include any

type of transportation carrier, such as, e.g., a water vessel like a boat, barge or ship, an aircraft like an

airplane or helicopter, a ground vehicle like a motorcycle, car, truck or train, A surface area may be made

of any material including brass, copper, aluminum, stainless steel, carbon steel, rubber, plastic, glass,

wood, painted surface, or any combination thereof. A surface area includes, without limitation, a table top,

counter top, floor, wall, ceiling, window, bed, gurney, door, door handle, shower, bath, sink, faucet, toilet,

toilet seat, drain, equipment, machinery, personal protective gear, personal biohazard gear, and the like. A

surface area may comprise a medical, dental, pharmaceutical, veterinary or mortuary device. A surface

area may comprise human skin.

[254] A composition or component disclosed herein can be applied to a hard surface area according to

a method disclosed herein as often as needed and/or desired. A composition disclosed herein can be

applied to a hard surface area daily, every other day, every third of day, once a week, multiple times per

week, once a month, multiple times per month, once a year or multiple times per year, as desired. A

composition disclosed herein can be applied to a hard surface area multiple times per day, e.g., twice a

day, three times a day, four time a day, five times a day, six times a day or as often as desired.

[255] The presently disclosed compositions are useful in any application involving treating and/or

providing relief to an individual. The presently disclosed compositions are useful in any application involving medical use, veterinarian use, or both. In one embodiment, a composition disclosed herein is useful in any application were hypochlorous acid is applied or administered.

[256] In one embodiment, the presently disclosed compositions are useful in any application involving

treating and/or providing relief to a tissue in an individual. For example, the compositions disclosed herein

can be used to treat and/or provide relief from a wound by washing or irrigating the tissue that minimizes

the introduction of microbes to the tissue thereby facilitating the healing of the tissue.

[257] In one embodiment, the presently disclosed compositions are useful in any application involving

treating and/or providing relief from a wound in an individual. For example, the compositions disclosed

herein can be used to treat and/or provide relief from a wound by washing or irrigating the wound that

minimizes the introduction of microbes to the wound thereby facilitating the healing of the wound. A wound

can be an open wound, a closed wound or a burn. Non-limiting examples, of an open wound include a

laceration, an abrasion, an incision, a puncture, an avulsion, an ulcer, and a tear. Non-limiting examples,

of a closed wound includes a bruise, a contusion and a hematoma.

[258] Aspects of the present specification disclose a method to wash or irrigate a wound in an individual.

Such methods are designed to prevent introduction of microbes to a wound due to adverse levels of

comprises applying a composition disclosed herein to the wound of an individual, wherein application of

the composition washes or irrigates the wound.

[259] In aspects of this embodiment, a composition disclosed herein is for use in washing or irrigating a

wound in an individual. In other aspects of this embodiment, a use of a disclosed composition is to wash

or irrigate a wound in an individual. In yet aspects of this embodiment, a use of a disclosed composition is

in the manufacture of a medicament to wash or irrigate a wound in an individual.

[260] In aspects of this embodiment, a composition disclosed herein is for use for a therapeutic effect,

such as angiogenesis, reduction of inflammation, pain, itch, irritation, or pressure, reduction of histidine

production, oxygenation of tissues, or soothing. As such, methods of treatment are also disclosed.

[261] Other aspects of the present specification disclose a method to treat and/or provide relief from a

wound in an individual. Such methods are designed to prevent introduction of microbes to a wound due to

adverse levels of contamination in a composition disclosed herein. In aspects of this embodiment, a method

disclosed herein comprises applying a composition disclosed herein to an individual, wherein application

of the composition promotes healing of a wound.

[262] In aspects of this embodiment, a composition disclosed herein is for use in treating and/or providing

relief from a wound in an individual. In other aspects of this embodiment, a use of a disclosed composition

is to treat and/or provide relief from a wound in an individual. In still aspects of this embodiment, a use of

PCT/US2020/037665 105

a disclosed composition is in the manufacture of a medicament to treat and/or provide relief from a wound

in an individual.

[263] In an embodiment, a method disclosed herein does not disinfect or sterilize a wound.

[264] In one embodiment, the presently disclosed compositions are useful in any application involving a

microbial infection in an individual. A microbial infection includes a viral infection, a bacterial infection and

a fungal infection. A microbial infection can be an external infection on, e.g., a surface area of an individual

or an internal infection, e.g., a mercer infection or sepsis, located in the body or body cavity of an individual.

[265] Aspects of the present specification disclose a method to wash or irrigate an infection in an

individual. Such methods are designed to prevent introduction of microbes to an infection due to adverse

levels of contamination in a composition disclosed herein. In aspects of this embodiment, a method

of the composition washes or irrigates the infection.

[266] In aspects of this embodiment, a composition disclosed herein is for use in washing or irrigating an

infection in an individual. In other aspects of this embodiment, a use of a disclosed composition is to wash

or irrigate an infection in an individual. In yet aspects of this embodiment, a use of a disclosed composition

is in the manufacture of a medicament to wash or irrigate an infection in an individual. An infection can be

a microbial infection.

[267] Aspects of the present specification disclose a method of treating and/or providing relief from an

infection in an individual. Such methods are designed to prevent introduction of microbes to an infection

due to adverse levels of contamination in a composition disclosed herein. In aspects of this embodiment,

a method disclosed herein comprises applying a composition disclosed herein to an individual, wherein

application of the composition treats and/or provides relief from the infection.

[268] In aspects of this embodiment, a composition disclosed herein is for use in treating and/or providing

relief from an infection in an individual. In other aspects of this embodiment, a use of a disclosed

composition is to treat and/or provide relief from an infection in an individual. In yet aspects of this

embodiment, a use of a disclosed composition is in the manufacture of a medicament to treat and/or provide

relief from an infection in an infection.

[269] In an embodiment, a method disclosed herein does not disinfect or sterilize an infection.

[270] In one embodiment, a composition disclosed herein is used to wash or irrigate or treat and/or

provide relief for a body part of an individual. Non-limiting examples of uses for a composition disclosed

herein include washing or irrigating or treating and/or providing relief to tissue in wound care, pre-operative

preparation, surgery or other invasive procedure, and post-operative care, washing or irrigating or treating

and/or providing relief to a skin region in dermatological or other healthcare applications, washing or

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irrigating or treating and/or providing relief to a body cavity, including, without limitation, an oral cavity, a

nasal cavity, an otic cavity and a pulmonary cavity, and an abdominal cavity, washing or irrigating or treating

and/or providing relief to gums or tooth in dental applications, and washing or irrigating or treating and/or

providing relief to the eye in ophthalmological applications.

[271] A dermatological application refers to washing or irrigating or treating and/or providing relief to a

skin region of an individual of a microbial infection, such as, e.g., a viral, bacterial or fungal infection. Non-

limiting examples of a microbial infection of a skin region include urinary tract infection, eye lid wash,

cataract treatment, warts, cutaneous leishmaniasis, candidal vulvovagititis, derrmatophytoses,

bromhidrosis, pityriasis versicolor, acne vulgarish, rosasea, hydradenitis suppurativa, psoriasis, eczema,

alopecia areata, oral lichen planus, xeroderma pigmentosum, actinic keratoses, melasma, keloids, and anti-

aging.

[272] An ophthalmological application refers to washing or irrigating or treating and/or providing relief to

an eye of an individual of a microbial infection, such as, e.g., a viral, bacterial or fungal infection. Non-

limiting examples of a microbial infection of an eye include a bacterial conjunctivitis, a viral conjunctivitis,

an epidemic keratoconjunctivitis, a pharyngoconjunctival fever, a stye, a blephartis, an episcleritis, a

keratitis, a trachoma, and a corneal ulcer.

[273] The presently disclosed compositions are useful in any application involving treating and/or

providing relief from of an inflammation, an itch, an irritation, a pressure, an ache and/or a pain in an

individual.

[274] Aspects of the present specification disclose a method to wash or irrigate an inflammation in an

individual. Such methods are designed to prevent introduction of microbes to an inflamed site due to

of the composition washes or irrigates the inflammation.

[275] In aspects of this embodiment, a composition disclosed herein is for use in washing or treating

and/or providing relief from an inflammation in an individual. In other aspects of this embodiment, a use of

a disclosed composition is to wash or treat and/or provide relief from an inflammation in an individual. In

yet aspects of this embodiment, a use of a disclosed composition is in the manufacture of a medicament to

wash or irrigate an inflammation.

[276] Aspects of the present specification disclose a method of treating and/or providing relief from an

inflammation in an individual. Such methods are designed to prevent introduction of microbes to an

inflamed site due to adverse levels of contamination in a composition disclosed herein. In aspects of this

embodiment, a method disclosed herein comprises applying a composition disclosed herein to an

individual, wherein application of the composition treats and/or provides relief from the inflammation.

[277] In aspects of this embodiment, a composition disclosed herein is for use in treating and/or providing

relief from an inflammation in an individual. In other aspects of this embodiment, a use of a disclosed

composition is to treat and/or provide relief from an inflammation in an individual. In yet aspects of this

relief from an inflammation.

[278] In an embodiment, a method disclosed herein does not disinfect or sterilize an inflammation.

[279] The disclosed composition use active ingredients in a microbiostatic effective amount that are non-

toxic and non-irritating to an individual. As such. the presently disclosed compositions are useful in enteral

and parenteral applications, including oral, injectable and topical applications. For example, the

compositions disclosed herein can be used to wash or irrigate a body region injured by a wound or infected

with a microbe.

[280] In an aspect of this embodiment, a method disclosed herein comprises applying a composition

disclosed herein to an individual for a specified amount of time, wherein application results in the reduction

or inhibition of microbial growth in a microbial infection in the individual. Other aspects of the present

specification disclose a composition disclosed herein for use to reduce or inhibit microbial growth in a

microbial infection in an individual. Yet other aspects of the present specification disclose a use of a

disclosed composition to reduce or inhibit microbial growth in a microbial infection in an individual. Still

other aspects of the present specification disclose a composition disclosed herein for the manufacture of a

medicament to reduce or inhibit microbial growth in a microbial infection in an individual.

[281] A method and/or use disclosed herein applies a composition disclosed herein to an individual. An

individual refers to any animal including, without limitation, a fish, an amphibian, a bird and a mammal. A

mammal includes a human, a horse, a cow, a sheep, a dog and a cat. As such, a method disclosed herein

is for human use as well as veterinarian use.

[282] In applications to an individual, a composition disclosed herein can be applied to a skin surface or

can be internally administered. In one embodiment, a composition disclosed herein is applied topically to

a skin region of an individual in order to reduce or inhibit microbial growth in the skin region. A skin region,

includes, without limitation, the face, forehead, lips, scalp, neck, shoulder, arms, hands, thighs, legs, knees,

feet, chest, breast, back, groin, buttocks, and the like.

[283] In one embodiment, a composition disclosed herein is internally administered to an individual. Such

routes of administration include enteral routes of administration and parenteral routes of administration.

[284] A composition disclosed herein can be applied according to a method disclosed herein to a skin

region. Application of a composition disclosed herein can be by rubbing, pouring, sprinkling, or spraying

on, or otherwise applied to the human body. A composition disclosed herein can be applied by introducing

the composition into or onto a solid support such as, e.g., a wipe, a towelette, a towel, a mitt, a glove, or a mask and then applying the composition to a skin region. A composition disclosed herein can be applied by using a delivery device, such as, e.g., an aeroso dispenser, a pump spray, a trigger spray, a squeeze bottle, a topical patch, a transdermal patch, or a dermal implant to apply the composition to a skin region.

[285] A composition disclosed herein can be applied to an individual according to a method disclosed

herein as often as needed and/or desired. A composition disclosed herein can be applied to an individual

daily, every other day, every third of day, once a week, multiple times per week, once a month, multiple

times per month, once a year or multiple times per year, as desired. A composition disclosed herein can be

applied to an individual multiple times per day, e.g., twice a day, three times a day, four time a day, five

times a day, six times a day or as often as desired.

[286] The presently disclosed compositions are useful in food product, poultry, meat, vegetable

production. For example, the compositions disclosed herein can be used to reduce or inhibit microbial

growth on or in equipment, appliances or machinery, tables, rooms, including floors, ceilings and walls and

any other hard surface from microbial contamination.

[287] The presently disclosed compositions are useful in residential, commercial or industrial settings

using equipment, appliances, machinery or any other apparatus using water in the course of its operation.

For example, the compositions disclosed herein can be used to reduce or inhibit microbial growth on or in

equipment, appliances or machinery, such as, e,g., refrigerators, freezers, dishwashers, clothes washers,

water heaters, air conditioners, cooling towers or any apparatus using a humidifier.

[288] The presently disclosed compositions are useful in formulations on plants as a preservative or

pesticide. For example, the compositions disclosed herein can be used to reduce or inhibit microbial growth

on or in a plant injured by a wound or infected with a microbe.

[289] The presently disclosed compositions are useful in formulations on cut flowers to prolong freshness

and health. For example, the compositions disclosed herein can be used to reduce or inhibit microbial

growth on or in a cut flower at a wound or to prevent microbial infection.

[290] A disclosed method and/or use applies a composition disclosed herein for specified amount of time.

In one embodiment, a specified amount of time is a time sufficient to reduce or inhibit microbial growth on

or in a medical device, a surface, or an individual. In another embodiment, a specified amount of time is a

time sufficient to wash or irrigate a medical device, a surface, or an individual.

[291] In aspects of this embodiment, a composition disclosed herein is applied to a device, like a medical

device, a surface, or an individual for, e.g., about 1 minute, about 5 minutes, about 10 minutes, about 15

minutes, about 20 minutes, about 25 minutes, about 30 minutes, about 35 minutes, about 40 minutes, about

45 minutes, about 50 minutes, about 55 minutes, about 60 minutes, about 70 minutes, about 80 minutes,

about 90 minutes, about 100 minutes, about 110 minutes, or about 120 minutes. In other aspects of this

embodiment, a composition disclosed herein is applied to a device, like a medical device, a surface, or an

WO wo 2020/252433 PCT/US2020/037665 109

individual for, e.g., at least 1 minute, at least 5 minutes, at least 10 minutes, at least 15 minutes, at least 20

minutes, at least 25 minutes, at least 30 minutes, at least 35 minutes, at least 40 minutes, at least 45

minutes, at least 50 minutes, at least 55 minutes, at least 60 minutes, at least 70 minutes, at least 80

minutes, at least 90 minutes, at least 100 minutes, at least 110 minutes, or at least 120 minutes. In yet

other aspects of this embodiment, a composition disclosed herein is applied to device, like a medical device,

a surface, or an individual for, e.g., at most 1 minute, at most 5 minutes, at most 10 minutes, at most 15

minutes, at most 20 minutes, at most 25 minutes, at most 30 minutes, at most 35 minutes, at most 40

minutes, at most 45 minutes, at most 50 minutes, at most 55 minutes, at most 60 minutes, at most 70

minutes, at most 80 minutes, at most 90 minutes, at most 100 minutes, at most 110 minutes, or at most

120 minutes.

[292] In still other aspects of this embodiment, a composition disclosed herein is applied to a device, like

a medical device, a surface, or an individual for, e.g., about 1 minute to about 5 minutes, about 1 minute to

about 10 minutes, about 1 minute to about 15 minutes, about 1 minute to about 20 minutes, about 1 minute

to about 25 minutes, about 1 minute to about 30 minutes, about 1 minute to about 35 minutes, about 1

minute to about 40 minutes, about 1 minute to about 45 minutes, about 1 minute to about 50 minutes, about

1 minute to about 55 minutes, about 1 minute to about 60 minutes, about 5 minutes to about 10 minutes,

about 5 minutes to about 15 minutes, about 5 minutes to about 20 minutes, about 5 minutes to about 25

minutes, about 5 minutes to about 30 minutes, about 5 minutes to about 35 minutes, about 5 minutes to

about 40 minutes, about 5 minutes to about 45 minutes, about 5 minutes to about 50 minutes, about 5

minutes to about 55 minutes, about 5 minutes to about 60 minutes, about 5 minutes to about 70 minutes,

about 5 minutes to about 80 minutes, about 5 minutes to about 90 minutes, about 5 minutes to about 100

minutes, about 5 minutes to about 110 minutes, about 5 minutes to about 120 minutes, about 10 minutes

to about 15 minutes, about 10 minutes to about 20 minutes, about 10 minutes to about 25 minutes, about

10 minutes to about 30 minutes, about 10 minutes to about 35 minutes, about 10 minutes to about 40

minutes, about 10 minutes to about 45 minutes, about 10 minutes to about 50 minutes, about 10 minutes

to about 55 minutes, about 10 minutes to about 60 minutes, about 10 minutes to about 70 minutes, about

10 minutes to about 80 minutes, about 10 minutes to about 90 minutes, about 10 minutes to about 100

minutes, about 10 minutes to about 110 minutes, about 10 minutes to about 120 minutes, about 15 minutes

to about 20 minutes, about 15 minutes to about 25 minutes, about 15 minutes to about 30 minutes, about

15 minutes to about 35 minutes, about 15 minutes to about 40 minutes, about 15 minutes to about 45

minutes, about 15 minutes to about 50 minutes, about 15 minutes to about 55 minutes, about 15 minutes

to about 60 minutes, about 15 minutes to about 70 minutes, about 15 minutes to about 80 minutes, about

15 minutes to about 90 minutes, about 15 minutes to about 100 minutes, about 15 minutes to about 110

minutes, about 15 minutes to about 120 minutes, about 20 minutes to about 25 minutes, about 20 minutes

to about 30 minutes, about 20 minutes to about 35 minutes, about 20 minutes to about 40 minutes, about

20 minutes to about 45 minutes, about 20 minutes to about 50 minutes, about 20 minutes to about 55

minutes, about 20 minutes to about 60 minutes, about 20 minutes to about 70 minutes, about 20 minutes

to about 80 minutes, about 20 minutes to about 90 minutes, about 20 minutes to about 100 minutes, about

20 minutes to about 110 minutes, about 20 minutes to about 120 minutes, about 25 minutes to about 30

minutes, about 25 minutes to about 35 minutes, about 25 minutes to about 40 minutes, about 25 minutes

PCT/US2020/037665 110

to about 45 minutes, about 25 minutes to about 50 minutes, about 25 minutes to about 55 minutes, about

25 minutes to about 60 minutes, about 25 minutes to about 70 minutes, about 25 minutes to about 80

minutes, about 25 minutes to about 90 minutes, about 25 minutes to about 100 minutes, about 25 minutes

to about 110 minutes, about 25 minutes to about 120 minutes, about 30 minutes to about 35 minutes, about

30 minutes to about 40 minutes, about 30 minutes to about 45 minutes, about 30 minutes to about 50

minutes, about 30 minutes to about 55 minutes, about 30 minutes to about 60 minutes, about 30 minutes

to about 70 minutes, about 30 minutes to about 80 minutes, about 30 minutes to about 90 minutes, about

30 minutes to about 100 minutes, about 30 minutes to about 110 minutes, about 30 minutes to about 120

minutes, about 35 minutes to about 40 minutes, about 35 minutes to about 45 minutes, about 35 minutes

to about 50 minutes, about 35 minutes to about 55 minutes, about 35 minutes to about 60 minutes, about

35 minutes to about 70 minutes, about 35 minutes to about 80 minutes, about 35 minutes to about 90

minutes, about 35 minutes to about 100 minutes, about 35 minutes to about 110 minutes, about 35 minutes

to about 120 minutes, about 40 minutes to about 45 minutes, about 40 minutes to about 50 minutes, about

40 minutes to about 55 minutes, about 40 minutes to about 60 minutes, about 40 minutes to about 70

minutes, about 40 minutes to about 80 minutes, about 40 minutes to about 90 minutes, about 40 minutes

to about 100 minutes, about 40 minutes to about 110 minutes, about 40 minutes to about 120 minutes,

about 45 minutes to about 50 minutes, about 45 minutes to about 55 minutes, about 45 minutes to about

60 minutes, about 45 minutes to about 70 minutes, about 45 minutes to about 80 minutes, about 45 minutes

to about 90 minutes, about 45 minutes to about 100 minutes, about 45 minutes to about 110 minutes, about

45 minutes to about 120 minutes, about 50 minutes to about 55 minutes, about 50 minutes to about 60

minutes, about 50 minutes to about 70 minutes, about 50 minutes to about 80 minutes, about 50 minutes

to about 90 minutes, about 50 minutes to about 100 minutes, about 50 minutes to about 110 minutes, about

50 minutes to about 120 minutes, about 55 minutes to about 60 minutes, about 55 minutes to about 70

minutes, about 55 minutes to about 80 minutes, about 55 minutes to about 90 minutes, about 55 minutes

to about 100 minutes, about 55 minutes to about 110 minutes, about 55 minutes to about 120 minutes,

about 60 minutes to about 70 minutes, about 60 minutes to about 80 minutes, about 60 minutes to about

90 minutes, about 60 minutes to about 100 minutes, about 60 minutes to about 110 minutes, about 60

minutes to about 120 minutes, about 70 minutes to about 80 minutes, about 70 minutes to about 90 minutes,

about 70 minutes to about 100 minutes, about 70 minutes to about 110 minutes, about 70 minutes to about

120 minutes, about 80 minutes to about 90 minutes, about 80 minutes to about 100 minutes, about 80

minutes to about 110 minutes, about 80 minutes to about 120 minutes, about 90 minutes to about 100

minutes, about 90 minutes to about 110 minutes, about 90 minutes to about 120 minutes, about 100

minutes to about 110 minutes, about 100 minutes to about 120 minutes, or about 110 minutes to about 120

minutes.

[293] The disclosed compositions, methods and uses are less harsh than conventional compositions,

methods and uses resulting in a longer lifetime use of a surface and/or device, equipment, machinery or

other apparatus.

[294] In aspects of this embodiment, a surface and/or device, equipment, machinery or other apparatus

can be exposed to a composition disclosed herein, e.g., about 50 times, about 60 times, about 70 times, about 80 times, about 90 times, about 100 times, about 110 times, about 120 times, about 130 times, about

140 times, about 150 times, about 160 times, about 170 times, about 180 times, about 190 times, about

200 times, about 210 times, about 220 times, about 230 times, about 240 times, about 250 times, about

260 times, about 270 times, about 280 times, about 290 times, or about 300 times. In other aspects of this

embodiment, a surface and/or device, equipment, machinery or other apparatus can be exposed to a

composition disclosed herein, e.g., at least 50 times, at least 60 times, at least 70 times, at least 80 times,

at least 90 times, at least 100 times, at least 110 times, at least 120 times, at least 130 times, at least 140

times, at least 150 times, at least 160 times, at least 170 times, at least 180 times, at least 190 times, at

least 200 times, at least 210 times, at least 220 times, at least 230 times, at least 240 times, at least 250

times, at least 260 times, at least 270 times, at least 280 times, at least 290 times, or at least 300 times. In

yet other aspects of this embodiment, a medical device can be cleaned, disinfected and/or sterilized, e.g.,

at most 50 times, at most 60 times, at most 70 times, at most 80 times, at most 90 times, at most 100 times,

at most 110 times, at most 120 times, at most 130 times, at most 140 times, at most 150 times, at most

160 times, at most 170 times, at most 180 times, at most 190 times, at most 200 times, at most 210 times,

at most 220 times, at most 230 times, at most 240 times, at most 250 times, at most 260 times, at most

270 times, at most 280 times, at most 290 times, or at most 300 times.

[295] In still other aspects of this embodiment, a surface and/or device, equipment, machinery or other

apparatus can be exposed to a composition disclosed herein, e.g., about 50 times to about 60 times, about

50 times to about 70 times, about 50 times to about 80 times, about 50 times to about 90 times, about 50

times to about 100 times, about 50 times to about 110 times, about 50 times to about 120 times, about 50

times to about 130 times, about 50 times to about 140 times, about 50 times to about 150 times, about 50

times to about 175 times, about 50 times to about 200 times, about 50 times to about 225 times, about 50

times to about 250 times, about 50 times to about 275 times, about 50 times to about 300 times, about 75

times to about 90 times, about 75 times to about 100 times, about 75 times to about 110 times, about 75

times to about 120 times, about 75 times to about 130 times, about 75 times to about 140 times, about 75

times to about 150 times, about 75 times to about 175 times, about 75 times to about 200 times, about 75

times to about 225 times, about 75 times to about 250 times, about 75 times to about 275 times, about 75

times to about 300 times, about 100 times to about 110 times, about 100 times to about 120 times, about

100 times to about 130 times, about 100 times to about 140 times, about 100 times to about 150 times,

about 100 times to about 175 times, about 100 times to about 200 times, about 100 times to about 225

times, about 100 times to about 250 times, about 100 times to about 275 times, about 100 times to about

300 times, about 125 times to about 150 times, about 125 times to about 175 times, about 125 times to

about 200 times, about 125 times to about 225 times, about 125 times to about 250 times, about 125 times

to about 275 times, about 125 times to about 300 times, about 150 times to about 175 times, about 150

times to about 200 times, about 150 times to about 225 times, about 150 times to about 250 times, about

150 times to about 275 times, about 150 times to about 300 times, about 175 times to about 200 times,

about 175 times to about 225 times, about 175 times to about 250 times, about 175 times to about 275

times, about 175 times to about 300 times, about 200 times to about 225 times, about 200 times to about

250 times, about 200 times to about 275 times, about 200 times to about 300 times, about 225 times to

WO wo 2020/252433 PCT/US2020/037665 112 112

about 250 times, about 225 times to about 275 times, about 225 times to about 300 times, about 250 times

to about 275 times, about 250 times to about 300 times, or about 275 times to about 300 times.

[296] Aspects of the present specification can also be described as follows:

1. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine.

2. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine and one or more quaternary compounds or silicon quaternary compounds.

3. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine and one or more guanide-containing compounds.

4. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine and one or more guanide-containing compounds and one or more surfactants.

5. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine, one or more quaternary compounds or silicon quaternary compounds. and one or more

guanide-containing compounds.

6. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine and one or more alcohols.

7. A composition comprising, consisting essentially of, or consisting of one or more alcohols and one or

more quaternary compounds or silicon quaternary compounds.

8. A composition comprising, consisting essentially of, or consisting of one or more alcohols and one or

more guanide-containing compounds.

9. The composition according to any one of embodiments 1-8 further comprising, consisting essentially

of, or consisting of one or more metallic particles.

10. The composition according to any one of embodiments 1-9 further comprising, consisting essentially

of, or consisting of one or more metal salts.

11. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine and one or more metallic particles.

12. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine and one or more metal salts.

13. A composition comprising, consisting essentially of, or consisting of hypochlorous acid or free available

chlorine, one or more metallic particles and one or more metal salts.

14. The composition according to embodiments 1-6 and 11-13, wherein the hypochlorous acid or free

available chlorine is in an amount of 0.01 ppm, 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm,

0.30 ppm, 0.35 ppm, 0.40 ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75

ppm, 0.80 ppm, 0.85 ppm, 0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30

ppm, 35 ppm, 40 ppm, 45 ppm, 50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm,

90 ppm, 95 ppm, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300

ppm, 325 ppm, 350 ppm, 375 ppm, 400 ppm, 425 ppm, 450 ppm, 475 ppm, 500 ppm, 525 ppm, 550

ppm, 575 ppm, 600 ppm, 625 ppm, 650 ppm, 675 ppm, 700 ppm, 725 ppm, 750 ppm, 775 ppm, 800

ppm, 825 ppm, 850 ppm, 875 ppm, 900 ppm, 925 ppm, 950 ppm, 975 ppm, 1,000 ppm, 1,025 ppm,

1,050 ppm, 1075 ppm, 1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm, 1,200 ppm, 1,225 ppm, 1,250

WO wo 2020/252433 PCT/US2020/037665 113

ppm, 1,275 ppm, 1,300 ppm, 1,325 ppm, 1,350 ppm, 1,375 ppm, 1,400 ppm, 1,425 ppm, 1,450 ppm,

1,475 ppm, 1,500 ppm, 1,600 ppm, 1,700 ppm, 1,800 ppm, 1,900 ppm, or 2,000 ppm or at least 0.01

ppm, at least 0.05 ppm, at least 0.10 ppm, at least 0.20 ppm, at least 0.30 ppm, at least 0.40 ppm, at

least 0.50 ppm, at least 0.60 ppm, at least 0.70 ppm, at least 0.80 ppm, at least 0.90 ppm, at least 1

ppm, at least 10 ppm, at least 20 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, at least 60

ppm, at least 70 ppm, at least 80 ppm, at least 90 ppm, at least 100 ppm, at least 125 ppm, at least

150 ppm, at least 175 ppm, at least 200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at

least 300 ppm, at least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425

ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least

575 ppm, at least 600 ppm, at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at

least 725 ppm, at least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm, at least 850

1,000 ppm, at least 1,025 ppm, at least 1,050 ppm, at least 1075 ppm, at least 1,100 ppm, at least

1,125 ppm, at least 1,150 ppm, at least 1,175 ppm, at least 1,200 ppm, at least 1,225 ppm, at least

1,250 ppm, at least 1,275 ppm, at least 1,300 ppm, at least 1,325 ppm, at least 1,350 ppm, at least

1,375 ppm, at least 1,400 ppm, at least 1,425 ppm, at least 1,450 ppm, at least 1,475 ppm, at least

1,500 ppm, at least 1,600 ppm, at least 1,700 ppm, at least 1,800 ppm, at least 1,900 ppm, or at least

2,000 ppm or at most 0.01 ppm, at most 0.05 ppm, at most 0.10 ppm, at most 0.20 ppm, at most 0.30

ppm, at most 0.40 ppm, at most 0.50 ppm, at most 0.60 ppm, at most 0.70 ppm, at most 0.80 ppm, at

most 0.90 ppm, at most 1 ppm, at most 10 ppm, at most 20 ppm, at most 30 ppm, at most 40 ppm, at

most 50 ppm, at most 60 ppm, at most 70 ppm, at most 80 ppm, at most 90 ppm, at most 100 ppm, at

most 125 ppm, at most 150 ppm, at most 175 ppm, at most 200 ppm, at most 225 ppm, at most 250

ppm, at most 275 ppm, at most 300 ppm, at most 325 ppm, at most 350 ppm, at most 375 ppm, at most

400 ppm, at most 425 ppm, at most 450 ppm, at most 475 ppm, at most 500 ppm, at most 525 ppm, at

most 550 ppm, at most 575 ppm, at most 600 ppm, at most 625 ppm, at most 650 ppm, at most 675

ppm, at most 700 ppm, at most 725 ppm, at most 750 ppm, at most 775 ppm, at most 800 ppm, at most

825 ppm, at most 850 ppm, at most 875 ppm, at most 900 ppm, at most 925 ppm, at most 950 ppm, at

most 975 ppm, at most 1,000 ppm, at most 1,025 ppm, at most 1,050 ppm, at most 1075 ppm, at most

1,100 ppm, at most 1,125 ppm, at most 1,150 ppm, at most 1,175 ppm, at most 1,200 ppm, at most

1,225 ppm, at most 1,250 ppm, at most 1,275 ppm, at most 1,300 ppm, at most 1,325 ppm, at most

1,350 ppm, at most 1,375 ppm, at most 1,400 ppm, at most 1,425 ppm, at most 1,450 ppm, at most

1,475 ppm, at most 1,500 ppm, at most 1,600 ppm, at most 1,700 ppm, at most 1,800 ppm, at most

1,900 ppm, or at most 2,000 ppm or about 0.01 ppm to about 0.1 ppm, about 0.01 ppm to about 0.2

ppm, about 0.01 ppm to about 0.25 ppm, about 0.01 ppm to about 0.3 ppm, about 0.01 ppm to about

0.4 ppm, about 0.01 ppm to about 0.5 ppm, about 0.01 ppm to about 0.1 ppm, about 0.01 ppm to about

0.5 ppm, about 0.01 ppm to about 1 ppm, about 0.01 ppm to about 5 ppm, about 0.01 ppm to about 10

ppm, about 0.01 ppm to about 15 ppm, about 0.01 ppm to about 20 ppm, about 0.01 ppm to about 25

ppm, about 0.01 ppm to about 30 ppm, about 0.01 ppm to about 35 ppm, about 0.01 ppm to about 40

ppm, about 0.01 ppm to about 45 ppm, about 0.01 ppm to about 50 ppm, about 0.05 ppm to about 0.1

ppm, about 0.05 ppm to about 0.5 ppm, about 0.05 ppm to about 1 ppm, about 0.05 ppm to about 5

ppm, about 0.05 ppm to about 10 ppm, about 0.05 ppm to about 15 ppm, about 0.05 ppm to about 20 ppm, about 0.05 ppm to about 25 ppm, about 0.05 ppm to about 30 ppm, about 0.05 ppm to about 35 ppm, about 0.05 ppm to about 40 ppm, about 0.05 ppm to about 45 ppm, about 0.05 ppm to about 50 ppm, about 0.1 ppm to about 1 ppm, about 0.1 ppm to about 5 ppm, about 0.1 ppm to about 10 ppm, about 0.1 ppm to about 15 ppm, about 0.1 ppm to about 20 ppm, about 0.1 ppm to about 25 ppm, about

0.1 ppm to about 30 ppm, about 0.1 ppm to about 35 ppm, about 0.1 ppm to about 40 ppm, about 0.1

ppm to about 45 ppm, about 0.1 ppm to about 50 ppm, about 0.5 ppm to about 1 ppm, about 0.5 ppm

to about 5 ppm, about 0.5 ppm to about 10 ppm, about 0.5 ppm to about 15 ppm, about 0.5 ppm to

about 20 ppm, about 0.5 ppm to about 25 ppm, about 0.5 ppm to about 30 ppm, about 0.5 ppm to about

35 ppm, about 0.5 ppm to about 40 ppm, about 0.5 ppm to about 45 ppm, about 0.5 ppm to about 50

ppm, about 0.5 ppm to about 55 ppm, about 0.5 ppm to about 60 ppm, about 0.5 ppm to about 65 ppm,

about 0.5 ppm to about 70 ppm, about 0.5 ppm to about 75 ppm, about 0.5 ppm to about 80 ppm, about

0.5 ppm to about 85 ppm, about 0.5 ppm to about 90 ppm, about 0.5 ppm to about 95 ppm, about 0.5

ppm to about 100 ppm, about 0.75 ppm to about 20 ppm, about 0.75 ppm to about 25 ppm, about 0.75

ppm to about 30 ppm, about 0.75 ppm to about 35 ppm, about 0.75 ppm to about 40 ppm, about 0.75

ppm to about 45 ppm, about 0.75 ppm to about 50 ppm, about 0.75 ppm to about 55 ppm, about 0.75

ppm to about 60 ppm, about 0.75 ppm to about 65 ppm, about 0.75 ppm to about 70 ppm, about 0.75

ppm to about 75 ppm, about 0.75 ppm to about 80 ppm, about 0.75 ppm to about 85 ppm, about 0.75

ppm to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75 ppm to about 100 ppm, about 1

ppm to about 20 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 30 ppm, about 1 ppm to

about 35 ppm, about 1 ppm to about 40 ppm, about 1 ppm to about 45 ppm, about 1 ppm to about 50

ppm, about 1 ppm to about 55 ppm, about 1 ppm to about 60 ppm, about 1 ppm to about 65 ppm, about

1 ppm to about 70 ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 80 ppm, about 1 ppm to

about 85 ppm, about 1 ppm to about 90 ppm, about 1 ppm to about 95 ppm, about 1 ppm to about 100

ppm, about 5 ppm to about 20 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 30 ppm, about

5 ppm to about 35 ppm, about 5 ppm to about 40 ppm, about 5 ppm to about 45 ppm, about 5 ppm to

about 50 ppm, about 5 ppm to about 55 ppm, about 5 ppm to about 60 ppm, about 5 ppm to about 65

ppm, about 5 ppm to about 70 ppm, about 5 ppm to about 75 ppm, about 5 ppm to about 80 ppm, about

5 ppm to about 85 ppm, about 5 ppm to about 90 ppm, about 5 ppm to about 95 ppm, about 5 ppm to

about 100 ppm, about 10 ppm to about 20 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about

30 ppm, about 10 ppm to about 35 ppm, about 10 ppm to about 40 ppm, about 10 ppm to about 45

ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 55 ppm, about 10 ppm to about 60 ppm,

about 10 ppm to about 65 ppm, about 10 ppm to about 70 ppm, about 10 ppm to about 75 ppm, about

10 ppm to about 80 ppm, about 10 ppm to about 85 ppm, about 10 ppm to about 90 ppm, about 10

ppm to about 95 ppm, or about 10 ppm to about 100 ppm, about 1 ppm to about 25 ppm, about 1 ppm

to about 50 ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 100 ppm, about 1 ppm to about

125 ppm, about 1 ppm to about 150 ppm, about 1 ppm to about 175 ppm, about 1 ppm to about 200

ppm, about 1 ppm to about 225 ppm, about 1 ppm to about 250 ppm, about 1 ppm to about 275 ppm,

about 1 ppm to about 300 ppm, about 1 ppm to about 325 ppm, about 1 ppm to about 350 ppm, about

1 ppm to about 375 ppm, about 1 ppm to about 400 ppm, about 10 ppm to about 25 ppm, about 10

ppm to about 50 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 100 ppm, about 10 ppm

to about 125 ppm, about 10 ppm to about 150 ppm, about 10 ppm to about 175 ppm, about 10 ppm to

WO wo 2020/252433 PCT/US2020/037665 115

about 200 ppm, about 10 ppm to about 225 ppm, about 10 ppm to about 250 ppm, about 10 ppm to

about 275 ppm, about 10 ppm to about 300 ppm, about 10 ppm to about 325 ppm, about 10 ppm to

about 350 ppm, about 10 ppm to about 375 ppm, about 10 ppm to about 400 ppm, about 25 ppm to

about 50 ppm, about 25 ppm to about 75 ppm, about 25 ppm to about 100 ppm, about 25 ppm to about

125 ppm, about 25 ppm to about 150 ppm, about 25 ppm to about 175 ppm, about 25 ppm to about

200 ppm, about 25 ppm to about 225 ppm, about 25 ppm to about 250 ppm, about 25 ppm to about

275 ppm, about 25 ppm to about 300 ppm, about 25 ppm to about 325 ppm, about 25 ppm to about

350 ppm, about 25 ppm to about 375 ppm, about 25 ppm to about 400 ppm, about 50 ppm to about 75

ppm, about 50 ppm to about 100 ppm, about 50 ppm to about 125 ppm, about 50 ppm to about 150

ppm, about 50 ppm to about 175 ppm, about 50 ppm to about 200 ppm, about 50 ppm to about 225

ppm, about 50 ppm to about 250 ppm, about 50 ppm to about 275 ppm, about 50 ppm to about 300

ppm, about 50 ppm to about 325 ppm, about 50 ppm to about 350 ppm, about 50 ppm to about 375

ppm, about 50 ppm to about 400 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125

ppm, about 75 ppm to about 150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200

ppm, about 75 ppm to about 225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275

ppm, about 75 ppm to about 300 ppm, about 75 ppm to about 325 ppm, about 75 ppm to about 350

ppm, about 75 ppm to about 375 ppm, about 75 ppm to about 400 ppm, about 100 ppm to about 125

ppm, about 100 ppm to about 150 ppm, about 100 ppm to about 175 ppm, about 100 ppm to about 200

ppm, about 100 ppm to about 225 ppm, about 100 ppm to about 250 ppm, about 100 ppm to about 275

ppm, about 100 ppm to about 300 ppm, about 100 ppm to about 325 ppm, about 100 ppm to about 350

ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400 ppm, about 150 ppm to about 175

ppm, about 150 ppm to about 200 ppm, about 150 ppm to about 225 ppm, about 150 ppm to about 250

ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300 ppm, about 150 ppm to about 325

ppm, about 150 ppm to about 350 ppm, about 150 ppm to about 375 ppm, about 150 ppm to about 400

ppm, about 200 ppm to about 225 ppm, about 200 ppm to about 250 ppm, about 200 ppm to about 275

ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 325 ppm, about 200 ppm to about 350

ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400 ppm, about 200 ppm to about 425

ppm, about 200 ppm to about 450 ppm, about 200 ppm to about 475 ppm, about 200 ppm to about 500

ppm, about 200 ppm to about 525 ppm, about 200 ppm to about 550 ppm, about 200 ppm to about 575

ppm, about 200 ppm to about 600 ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300

ppm, about 250 ppm to about 325 ppm, about 250 ppm to about 350 ppm, about 250 ppm to about 375

ppm, about 250 ppm to about 400 ppm, about 250 ppm to about 425 ppm, about 250 ppm to about 450

ppm, about 250 ppm to about 475 ppm, about 250 ppm to about 500 ppm, about 250 ppm to about 525

ppm, about 250 ppm to about 550 ppm, about 250 ppm to about 575 ppm, about 250 ppm to about 600

ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375

ppm, about 300 ppm to about 400 ppm, about 300 ppm to about 425 ppm, about 300 ppm to about 450

ppm, about 300 ppm to about 475 ppm, about 300 ppm to about 500 ppm, about 300 ppm to about 525

ppm, about 300 ppm to about 550 ppm, about 300 ppm to about 575 ppm, about 300 ppm to about 600

ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, about 375 ppm to about 400

ppm, about 350 ppm to about 425 ppm, about 350 ppm to about 450 ppm, about 350 ppm to about 475

ppm, about 350 ppm to about 500 ppm, about 350 ppm to about 525 ppm, about 350 ppm to about 550 ppm, about 350 ppm to about 575 ppm, about 350 ppm to about 600 ppm, about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, about 400 ppm to about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about 400 ppm to about 1,000 ppm, about 400 ppm to about

1,100 ppm, about 400 ppm to about 1,200 ppm, about 400 ppm to about 1,300 ppm, about 400 ppm to

about 1,400 ppm, about 400 ppm to about 1,500 ppm, about 400 ppm to about 1,600 ppm, about 400

ppm to about 1,700 ppm, about 400 ppm to about 1,800 ppm, about 400 ppm to about 1,900 ppm,

about 400 ppm to about 2,000 ppm, about 500 ppm to about 600 ppm, about 500 ppm to about 700

ppm, about 500 ppm to about 800 ppm, about 500 ppm to about 900 ppm, about 500 ppm to about

1,000 ppm, about 500 ppm to about 1,100 ppm, about 500 ppm to about 1,200 ppm, about 500 ppm to

about 1,300 ppm, about 500 ppm to about 1,400 ppm, about 500 ppm to about 1,500 ppm, about 500

ppm to about 1,600 ppm, about 500 ppm to about 1,700 ppm, about 500 ppm to about 1,800 ppm,

about 500 ppm to about 1,900 ppm, about 500 ppm to about 2,000 ppm, about 600 ppm to about 700

ppm, about 600 ppm to about 800 ppm, about 600 ppm to about 900 ppm, about 600 ppm to about

1,000 ppm, about 600 ppm to about 1,100 ppm, about 600 ppm to about 1,200 ppm, about 600 ppm to

about 1,300 ppm, about 600 ppm to about 1,400 ppm, about 600 ppm to about 1,500 ppm, about 600

ppm to about 1,600 ppm, about 600 ppm to about 1,700 ppm, about 600 ppm to about 1,800 ppm,

about 600 ppm to about 1,900 ppm, about 600 ppm to about 2,000 ppm, about 700 ppm to about 800

ppm, about 700 ppm to about 900 ppm, about 700 ppm to about 1,000 ppm, about 700 ppm to about

1,100 ppm, about 700 ppm to about 1,200 ppm, about 700 ppm to about 1,300 ppm, about 700 ppm to

about 1,400 ppm, about 700 ppm to about 1,500 ppm, about 700 ppm to about 1,600 ppm, about 700

ppm to about 1,700 ppm, about 700 ppm to about 1,800 ppm, about 700 ppm to about 1,900 ppm,

about 700 ppm to about 2,000 ppm, about 800 ppm to about 900 ppm, about 800 ppm to about 1,000

ppm, about 800 ppm to about 1,100 ppm, about 800 ppm to about 1,200 ppm, about 800 ppm to about

1,300 ppm, about 800 ppm to about 1,400 ppm, about 800 ppm to about 1,500 ppm, about 800 ppm to

about 1,600 ppm, about 800 ppm to about 1,700 ppm, about 800 ppm to about 1,800 ppm, about 800

ppm to about 1,900 ppm, about 800 ppm to about 2,000 ppm, about 900 ppm to about 1,000 ppm,

about 900 ppm to about 1,100 ppm, about 900 ppm to about 1,200 ppm, about 900 ppm to about 1,300

ppm, about 900 ppm to about 1,400 ppm, about 900 ppm to about 1,500 ppm, about 900 ppm to about

1,600 ppm, about 900 ppm to about 1,700 ppm, about 900 ppm to about 1,800 ppm, about 900 ppm to

about 1,900 ppm, about 900 ppm to about 2,000 ppm, about 1,000 ppm to about 1,100 ppm, about

1,000 ppm to about 1,200 ppm, about 1,000 ppm to about 1,300 ppm, about 1,000 ppm to about 1,400

ppm, about 1,000 ppm to about 1,500 ppm, about 1,000 ppm to about 1,600 ppm, about 1,000 ppm to

about 1,700 ppm, about 1,000 ppm to about 1,800 ppm, about 1,000 ppm to about 1,900 ppm, about

1,000 ppm to about 2,000 ppm, about 1,100 ppm to about 1,200 ppm, about 1,100 ppm to about 1,300

ppm, about 1,100 ppm to about 1,400 ppm, about 1,100 ppm to about 1,500 ppm, about 1,100 ppm to

about 1,600 ppm, about 1,100 ppm to about 1,700 ppm, about 1,100 ppm to about 1,800 ppm, about

1,100 ppm to about 1,900 ppm, about 1,100 ppm to about 2,000 ppm, about 1,200 ppm to about 1,300

ppm, about 1,200 ppm to about 1,400 ppm, about 1,200 ppm to about 1,500 ppm, about 1,200 ppm to

about 1,600 ppm, about 1,200 ppm to about 1,700 ppm, about 1,200 ppm to about 1,800 ppm, about

1,200 ppm to about 1,900 ppm, about 1,200 ppm to about 2,000 ppm, about 1,300 ppm to about 1,400

ppm, about 1,300 ppm to about 1,500 ppm, about 1,300 ppm to about 1,600 ppm, about 1,300 ppm to about 1,700 ppm, about 1,300 ppm to about 1,800 ppm, about 1,300 ppm to about 1,900 ppm, about

1,300 ppm to about 2,000 ppm, about 1,400 ppm to about 1,500 ppm, about 1,400 ppm to about 1,600

ppm, about 1,400 ppm to about 1,700 ppm, about 1,400 ppm to about 1,800 ppm, about 1,400 ppm to

about 1,900 ppm, about 1,400 ppm to about 2,000 ppm, about 1,500 ppm to about 1,600 ppm, about

1,500 ppm to about 1,700 ppm, about 1,500 ppm to about 1,800 ppm, about 1,500 ppm to about 1,900

ppm, about 1,500 ppm to about 2,000 ppm, about 1,600 ppm to about 1,700 ppm, about 1,600 ppm to

about 1,800 ppm, about 1,600 ppm to about 1,900 ppm, about 1,600 ppm to about 2,000 ppm, about

1,700 ppm to about 1,800 ppm, about 1,700 ppm to about 1,900 ppm, about 1,700 ppm to about 2,000

ppm, about 1,800 ppm to about 1,900 ppm, about 1,800 ppm to about 2,000 ppm, or about 1,900 ppm

to about 2,000 ppm.

15. The composition according to any one of embodiments 1-6 and 11-13, wherein the hypochlorous acid

or free available chlorine is in an amount of about 0.001%, about 0.005%, about 0.01%, about 0.05%,

0.17%, about 0.18%, about 0.19%, about 0.2%, about 0.25%, about 0.3%, about 0.35%, about 0.4%,

about 0.45%, or about 0.5% by weight of the composition, or at least 0.001%, at least 0.005%, at least

0.01%, at least 0.05%, at least 0.10%, at least 0.11%, at least 0.12%, at least 0.13%, at least 0.14%,

at least 0.15%, at least 0.16%, at least 0.17%, at least 0.18%, at least 0.19%, at least 0.2%, at least

composition, or at most 0.001%, at most 0.005%, at most 0.01%, at most 0.05%, at most 0.10%, at

most 0.11%, at most 0.12%, at most 0.13%, at most 0.14%, at most 0.15%, at most 0.16%, at most

0.17%, at most 0.18%, at most 0.19%, at most 0.2%, at most 0.25%, at most 0.3%, at most 0.35%, at

most 0.4%, at most 0.45%, or at most 0.5% by weight of the composition, or about 0.0010% to about

0.005%, about 0.0010% to about 0.010%, about 0.0010% to about 0.050%, about 0.0010% to about

0.10%, about 0.0010% to about 0.15%, about 0.0010% to about 0.20%, about 0.0010% to about 0.25%,

about 0.0010% to about 0.30%, about 0.0010% to about 0.35%, about 0.0010% to about 0.40%, about

0.0010% to about 0.45%, about 0.0010% to about 0.50%, about 0.010% to about 0.050%, about

0.010% to about 0.10%, about 0.010% to about 0.15%, about 0.010% to about 0.20%, about 0.010%

0.40%, about 0.010% to about 0.45%, about 0.010% to about 0.50%, about 0.10% to about 0.15%,

about 0.10% to about 0.20%, about 0.10% to about 0.25%, about 0.10% to about 0.30%, about 0.10%

to about 0.35%, about 0.10% to about 0.40%, about 0.10% to about 0.45%, about 0.10% to about

0.50%, about 0.20% to about 0.25%, about 0.20% to about 0.30%, about 0.20% to about 0.35%, about

0.20% to about 0.40%, about 0.20% to about 0.45%, about 0.20% to about 0.50%, about 0.30% to

about 0.40% to about 0.45%, about 0.40% to about 0.50%, or about 0.40% to about 0.50% by weight

of the composition.

16. The composition according to any one of embodiments 2, 5, 7, 14 or 15, wherein the one or more

quaternary compounds include one or more dialkyl quaternary compounds and/or one or more polyether fatty quaternary compounds or wherein the one or more silicon quaternary compounds

include one or more silicone dialkyl quaternary compounds and/or one or more silicone polyether fatty

quaternary compounds.

PCT/US2020/037665 118

17. The composition according to embodiment 15, wherein the one or more dialkyl quaternary compounds

include didodecyldimethylammonium chloride and di-n-alkyldimethyl ammonium chloride and/or

wherein the one or more silicone dialkyl quaternary compounds include dimethyloctadecyl[3

(trimethoxysilyl)propyl]ammonium chloride and tetradecyldimethyl-(3-trimethoxysilyl)propyl ammonium

chloride.

18. The composition according to any one of embodiments 2, 5, 7, or 14-17, wherein the one or more

quaternary compounds or silicon quaternary compounds are in an amount of about 0.01%, about

0.05%, about 0.075%, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%,

about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1.5%, about 2.0%, about 2.5%, about 3.0%,

about 4.0%, about 5.0%, about 6.0%, about 7.0%, about 7.5%, about 8.0%, about 9.0%, about 10.0%,

about 11.0%, about 12.0%, about 13.0%, about 14.0%, about 15.0%, about 16.0%, about 17.0%,

18.0%, about 19.0%, or about 20.0% by weight of the composition, or at least 0.01%, at least 0.05%,

at least 0.075%, at least 0.1%, at least 0.25%, at least 0.5%, at least 0.75%, at least 1.0%, at least

1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at least

7.0%, at least 7.5%, at least 8.0%, at least 9.0%, at least 10.0%, at least 11.0%, at least 12.0%, at least

13.0%, at least 14.0%, at least 15.0%, at least 16.0%, at least 17.0%, at least 18.0%, at least 19.0%,

or at least 20.0% by weight of the composition by weight of the composition, or at most 0.01%, at most

0.05%, at most 0.075%, at most 0.1%, at most 0.25%, at most 0.5%, at most 0.75%, at most 1.0%, at

most 1.5%, at most 2.0%, at most 2.5%, at most 3.0%, at most 4.0%, at most 5.0%, at most 6.0%, at

most 7.5%, at most 8.0%, at most 9.0%, at most 10.0%, at most 11.0%, at most 12.0%, at most 13.0%,

at most 14.0%, at most 15.0%, at most 16.0%, at most 17.0%, at most 18.0%, at most 19.0%, or at

most 20.0% by weight of the composition by weight of the composition, or about 0.1% to about 0.5%,

about 0.1% to about 0.75%, about 0.1% to about 1.0%, about 0.1% to about 1.5%, about 0.1% to about

2.0%, about 0.1% to about 2.5%, about 0.2% to about 0.5%, about 0.2% to about 0.75%, about 0.2%

to about 1.0%, about 0.2% to about 1.5%, about 0.2% to about 2.0%, about 0.2% to about 2.5%, about

0.5% to about 1.0%, about 0.5% to about 1.5%, about 0.5% to about 2.0%, about 0.5% to about 2.5%,

about 0.5% to about 3.0%, about 0.5% to about 4.0%, about 0.5% to about 5.0%, about 1.0% to about

2.0%, about 1.0% to about 3.0%, about 1.0% to about 4.0%, about 1.0% to about 5.0%, about 1.0% to

about 6.0%, about 1.0% to about 7.0%, about 1.0% to about 8.0%, about 1.0% to about 9.0%, about

1.0% to about 10%, about 1.0% to about 11%, about 1.0% to about 12%, about 1.0% to about 13%,

about 1.0% to about 14%, about 1.0% to about 15%, about 2.0% to about 3.0%, about 2.0% to about

4.0%, about 2.0% to about 5.0%, about 2.0% to about 6.0%, about 2.0% to about 7.0%, about 2.0% to

about 8.0%, about 2.0% to about 9.0%, about 2.0% to about 10%, about 2.0% to about 11%, about

2.0% to about 12%, about 2.0% to about 13%, about 2.0% to about 14%, about 2.0% to about 15%,

about 3.0% to about 4.0%, about 3.0% to about 5.0%, about 3.0% to about 6.0%, about 3.0% to about

7.0%, about 3.0% to about 8.0%, about 3.0% to about 9.0%, about 3.0% to about 10%, about 3.0% to

about 11%, about 3.0% to about 12%, about 3.0% to about 13%, about 3.0% to about 14%, about 3.0%

to about 15%, about 4.0% to about 5.0%, about 4.0% to about 6.0%, about 4.0% to about 7.0%, about

4.0% to about 8.0%, about 4.0% to about 9.0%, about 4.0% to about 10%, about 4.0% to about 11%,

about 4.0% to about 12%, about 4.0% to about 13%, about 4.0% to about 14%, about 4.0% to about

15%, about 5.0% to about 6.0%, about 5.0% to about 7.0%, about 5.0% to about 8.0%, about 5.0% to about 9.0%, about 5.0% to about 10%, about 5.0% to about 11%, about 5.0% to about 12%, about

5.0% to about 13%, about 5.0% to about 14%, about 5.0% to about 15%, about 6.0% to about 7.0%,

about 6.0% to about 8.0%, about 6.0% to about 9.0%, about 6.0% to about 10%, about 6.0% to about

11%, about 6.0% to about 12%, about 6.0% to about 13%, about 6.0% to about 14%, about 6.0% to

about 15%, about 7.0% to about 8.0%, about 7.0% to about 9.0%, about 7.0% to about 10%, about

7.0% to about 11%, about 7.0% to about 12%, about 7.0% to about 13%, about 7.0% to about 14%,

about 7.0% to about 15%, about 8.0% to about 9.0%, about 8.0% to about 10%, about 8.0% to about

11%, about 8.0% to about 12%, about 8.0% to about 13%, about 8.0% to about 14%, about 8.0% to

about 15%, about 9.0% to about 10%, about 9.0% to about 11%, about 9.0% to about 12%, about 9.0%

to about 13%, about 9.0% to about 14%, about 9.0% to about 15%, about 10% to about 11%, about

13% to about 15%, or 14% to about 15% by weight of the composition.

19. The composition according to any one of embodiments 2, 5, 7, or 14-17, wherein the one or more

quaternary compounds or silicon quaternary compounds is in an amount of 0.05 ppm, 0.10 ppm, 0.15

ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm, 0.40 ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm,

0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80 ppm, 0.85 ppm, 0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15

ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45 ppm, 50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm,

75 ppm, 80 ppm, 85 ppm, 90 ppm, 95 ppm, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm, 350 ppm, 375 ppm, 400 ppm, 425 ppm, 450 ppm, 475

ppm, 500 ppm, 525 ppm, 550 ppm, 575 ppm, 600 ppm, 625 ppm, 650 ppm, 675 ppm, 700 ppm, 725

ppm, 750 ppm, 775 ppm, 800 ppm, 825 ppm, 850 ppm, 875 ppm, 900 ppm, 925 ppm, 950 ppm, 975

ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm, 1075 ppm, 1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm,

1,200 ppm, 1,225 ppm, 1,250 ppm, 1,275 ppm, 1,300 ppm, 1,325 ppm, 1,350 ppm, 1,375 ppm, 1,400

ppm, 1,425 ppm, 1,450 ppm, 1,475 ppm, or 1,500 ppm or at least 0.05 ppm, at least 0.10 ppm, at least

0.20 ppm, at least 0.30 ppm, at least 0.40 ppm, at least 0.50 ppm, at least 0.60 ppm, at least 0.70 ppm,

at least 0.80 ppm, at least 0.90 ppm, at least 1 ppm, at least 10 ppm, at least 20 ppm, at least 30 ppm,

at least 40 ppm, at least 50 ppm, at least 60 ppm, at least 70 ppm, at least 80 ppm, at least 90 ppm, at

least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm, at least 225

ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350 ppm, at least

375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least 500 ppm, at

least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least 625 ppm, at least 650

ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775 ppm, at least

800 ppm, at least 825 ppm, at least 850 ppm, at least 875 ppm, at least 900 ppm, at least 925 ppm, at

least 950 ppm, at least 975 ppm, at least 1,000 ppm, at least 1,025 ppm, at least 1,050 ppm, at least

1075 ppm, at least 1,100 ppm, at least 1,125 ppm, at least 1,150 ppm, at least 1,175 ppm, at least

1,200 ppm, at least 1,225 ppm, at least 1,250 ppm, at least 1,275 ppm, at least 1,300 ppm, at least

1,325 ppm, at least 1,350 ppm, at least 1,375 ppm, at least 1,400 ppm, at least 1,425 ppm, at least

1,450 ppm, at least 1,475 ppm, or at least 1,500 ppm or at most 0.05 ppm, at most 0.10 ppm, at most

0.20 ppm, at most 0.30 ppm, at most 0.40 ppm, at most 0.50 ppm, at most 0.60 ppm, at most 0.70 ppm, at most 0.80 ppm, at most 0.90 ppm, at most 1 ppm, at most 10 ppm, at most 20 ppm, at most

30 ppm, at most 40 ppm, at most 50 ppm, at most 60 ppm, at most 70 ppm, at most 80 ppm, at most

90 ppm, at most 100 ppm, at most 125 ppm, at most 150 ppm, at most 175 ppm, at most 200 ppm, at

most 225 ppm, at most 250 ppm, at most 275 ppm, at most 300 ppm, at most 325 ppm, at most 350

ppm, at most 375 ppm, at most 400 ppm, at most 425 ppm, at most 450 ppm, at most 475 ppm, at most

500 ppm, at most 525 ppm, at most 550 ppm, at most 575 ppm, at most 600 ppm, at most 625 ppm, at

most 650 ppm, at most 675 ppm, at most 700 ppm, at most 725 ppm, at most 750 ppm, at most 775

ppm, at most 800 ppm, at most 825 ppm, at most 850 ppm, at most 875 ppm, at most 900 ppm, at most

925 ppm, at most 950 ppm, at most 975 ppm, at most 1,000 ppm, at most 1,025 ppm, at most 1,050

ppm, at most 1075 ppm, at most 1,100 ppm, at most 1,125 ppm, at most 1,150 ppm, at most 1,175

ppm, at most 1,200 ppm, at most 1,225 ppm, at most 1,250 ppm, at most 1,275 ppm, at most 1,300

ppm, at most 1,325 ppm, at most 1,350 ppm, at most 1,375 ppm, at most 1,400 ppm, at most 1,425

ppm, at most 1,450 ppm, at most 1,475 ppm, or at most 1,500 ppm or about 0.5 ppm to about 20 ppm,

0.5 ppm to about 40 ppm, about 0.5 ppm to about 45 ppm, about 0.5 ppm to about 50 ppm, about 0.5

ppm to about 55 ppm, about 0.5 ppm to about 60 ppm, about 0.5 ppm to about 65 ppm, about 0.5 ppm

to about 70 ppm, about 0.5 ppm to about 75 ppm, about 0.5 ppm to about 80 ppm, about 0.5 ppm to

about 85 ppm, about 0.5 ppm to about 90 ppm, about 0.5 ppm to about 95 ppm, about 0.5 ppm to about

100 ppm, about 0.75 ppm to about 20 ppm, about 0.75 ppm to about 25 ppm, about 0.75 ppm to about

30 ppm, about 0.75 ppm to about 35 ppm, about 0.75 ppm to about 40 ppm, about 0.75 ppm to about

45 ppm, about 0.75 ppm to about 50 ppm, about 0.75 ppm to about 55 ppm, about 0.75 ppm to about

60 ppm, about 0.75 ppm to about 65 ppm, about 0.75 ppm to about 70 ppm, about 0.75 ppm to about

75 ppm, about 0.75 ppm to about 80 ppm, about 0.75 ppm to about 85 ppm, about 0.75 ppm to about

90 ppm, about 0.75 ppm to about 95 ppm, about 0.75 ppm to about 100 ppm, about 1 ppm to about 20

ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 30 ppm, about 1 ppm to about 35 ppm, about

1 ppm to about 40 ppm, about 1 ppm to about 45 ppm, about 1 ppm to about 50 ppm, about 1 ppm to

about 55 ppm, about 1 ppm to about 60 ppm, about 1 ppm to about 65 ppm, about 1 ppm to about 70

ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 80 ppm, about 1 ppm to about 85 ppm, about

about 20 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 30 ppm, about 5 ppm to about 35

ppm, about 5 ppm to about 40 ppm, about 5 ppm to about 45 ppm, about 5 ppm to about 50 ppm, about

5 ppm to about 55 ppm, about 5 ppm to about 60 ppm, about 5 ppm to about 65 ppm, about 5 ppm to

about 70 ppm, about 5 ppm to about 75 ppm, about 5 ppm to about 80 ppm, about 5 ppm to about 85

ppm, about 5 ppm to about 90 ppm, about 5 ppm to about 95 ppm, about 5 ppm to about 100 ppm,

about 10 ppm to about 20 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 30 ppm, about

10 ppm to about 35 ppm, about 10 ppm to about 40 ppm, about 10 ppm to about 45 ppm, about 10

ppm to about 50 ppm, about 10 ppm to about 55 ppm, about 10 ppm to about 60 ppm, about 10 ppm

to about 65 ppm, about 10 ppm to about 70 ppm, about 10 ppm to about 75 ppm, about 10 ppm to

about 80 ppm, about 10 ppm to about 85 ppm, about 10 ppm to about 90 ppm, about 10 ppm to about

95 ppm, or about 10 ppm to about 100 ppm or about 1 ppm to about 25 ppm, about 1 ppm to about 50

ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 100 ppm, about 1 ppm to about 125 ppm,

WO wo 2020/252433 PCT/US2020/037665 121

about 1 ppm to about 150 ppm, about 1 ppm to about 175 ppm, about 1 ppm to about 200 ppm, about

1 ppm to about 225 ppm, about 1 ppm to about 250 ppm, about 1 ppm to about 275 ppm, about 1 ppm

to about 300 ppm, about 1 ppm to about 325 ppm, about 1 ppm to about 350 ppm, about 1 ppm to

about 375 ppm, about 1 ppm to about 400 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about

50 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 100 ppm, about 10 ppm to about 125

ppm, about 10 ppm to about 150 ppm, about 10 ppm to about 175 ppm, about 10 ppm to about 200

ppm, about 10 ppm to about 225 ppm, about 10 ppm to about 250 ppm, about 10 ppm to about 275

ppm, about 10 ppm to about 300 ppm, about 10 ppm to about 325 ppm, about 10 ppm to about 350

ppm, about 10 ppm to about 375 ppm, about 10 ppm to about 400 ppm, about 25 ppm to about 50

ppm, about 25 ppm to about 75 ppm, about 25 ppm to about 100 ppm, about 25 ppm to about 125

ppm, about 25 ppm to about 150 ppm, about 25 ppm to about 175 ppm, about 25 ppm to about 200

ppm, about 25 ppm to about 225 ppm, about 25 ppm to about 250 ppm, about 25 ppm to about 275

ppm, about 25 ppm to about 300 ppm, about 25 ppm to about 325 ppm, about 25 ppm to about 350

ppm, about 25 ppm to about 375 ppm, about 25 ppm to about 400 ppm, about 50 ppm to about 75

ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400 ppm, about 250 ppm to about 275

ppm, about 250 ppm to about 300 ppm, about 250 ppm to about 325 ppm, about 250 ppm to about 350

ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400 ppm, about 300 ppm to about 325

ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400

ppm, about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, about 400 ppm to about 700

ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about 400 ppm to about

1,000 ppm, about 400 ppm to about 1,100 ppm, about 400 ppm to about 1,200 ppm, about 400 ppm to

about 1,300 ppm, about 400 ppm to about 1,400 ppm, about 400 ppm to about 1,500 ppm, about 500 ppm to about 600 ppm, about 500 ppm to about 700 ppm, about 500 ppm to about 800 ppm, about 500 ppm to about 900 ppm, about 500 ppm to about 1,000 ppm, about 500 ppm to about 1,100 ppm, about

500 ppm to about 1,200 ppm, about 500 ppm to about 1,300 ppm, about 500 ppm to about 1,400 ppm,

about 500 ppm to about 1,500 ppm, about 600 ppm to about 700 ppm, about 600 ppm to about 800

ppm, about 600 ppm to about 900 ppm, about 600 ppm to about 1,000 ppm, about 600 ppm to about

1,100 ppm, about 600 ppm to about 1,200 ppm, about 600 ppm to about 1,300 ppm, about 600 ppm to

about 1,400 ppm, about 600 ppm to about 1,500 ppm, about 700 ppm to about 800 ppm, about 700

ppm to about 900 ppm, about 700 ppm to about 1,000 ppm, about 700 ppm to about 1,100 ppm, about

about 700 ppm to about 1,500 ppm, about 800 ppm to about 900 ppm, about 800 ppm to about 1,000

1,300 ppm, about 800 ppm to about 1,400 ppm, about 800 ppm to about 1,500 ppm, about 900 ppm to

about 1,000 ppm, about 900 ppm to about 1,100 ppm, about 900 ppm to about 1,200 ppm, about 900

ppm to about 1,300 ppm, about 900 ppm to about 1,400 ppm, about 900 ppm to about 1,500 ppm,

about 1,000 ppm to about 1,100 ppm, about 1,000 ppm to about 1,200 ppm, about 1,000 ppm to about

1,300 ppm, about 1,000 ppm to about 1,400 ppm, about 1,000 ppm to about 1,500 ppm, about 1,100

ppm to about 1,200 ppm, about 1,100 ppm to about 1,300 ppm, about 1,100 ppm to about 1,400 ppm,

about 1,100 ppm to about 1,500 ppm, about 1,200 ppm to about 1,300 ppm, about 1,200 ppm to about

1,400 ppm, about 1,200 ppm to about 1,500 ppm, about 1,300 ppm to about 1,400 ppm, about 1,300

ppm to about 1,500 ppm, or about 1,400 ppm to about 1,500 ppm.

20. The composition according to any one of embodiment 3-5, 8, or 14-19, wherein the one or more

guanide-containing compounds comprise, consist essentially of, or consist of a biguanide, a biguanide-

containing compound, a biguanidine, a biguanidine-containing compound, a triguanide, a triguanide-

containing compound, or any combination thereof.

21. The composition according to embodiment 20, wherein the one or more biguanide-containing

compounds include a polyhexamethylene biguanide (PHMB), a polyaminopropyl biguanide (PAPB), a

,1'-(1,6-Hexanediyl)bis{2-[N'-(2-ethylhexyl)carbamimidoyl]guanidine} (alexidine), a chlorhexidine, a

chlorhexidine gluconate, or any combination thereof.

22. The composition according to any one of embodiments 3-5, 8, or 14-21, wherein the one or more

guanide-containing compounds are in an amount of about 0.01%, about 0.05%, about 0.075%, about

0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about

0.9%, about 1.0%, about 1.5%, about 2.0%, about 2.5%, about 3.0%, about 4.0%, about 5.0%, about

6.0%, about 7.0%, about 7.5%, about 8.0%, about 9.0%, about 10.0%. about 11%, about 12%, about

13%, about 14%, about 15%, about 20%, about 25%, or about 30% by weight of the composition, or at

least 0.01%, at least 0.05%, at least 0.075%, at least 0.1%, at least 0.25%, at least 0.5%, at least

0.75%, at least 1.0%, at least 1.5%, at least 2.0%, at least 2.5%, at least 3.0%, at least 4.0%, at least

5.0%, at least 6.0%, at least 7.0%, at least 7.5%, at least 8.0%, at least 9.0%, at least 10.0%, at least

11%, at least 12%, at least 13%, at least 14%, at least 15%, at least 20%, at least 25%, or at least 30%

by weight of the composition, or at most 0.01%, at most 0.05%, at most 0.075%, at most 0.1%, at most

0.25%, at most 0.5%, at most 0.75%, at most 1.0%, at most 1.5%, at most 2.0%, at most 2.5%, at most

3.0%, at most 4.0%, at most 5.0%, at most 6.0%, at most 7.5%, at most 8.0%, at most 9.0%, at most

WO wo 2020/252433 PCT/US2020/037665 123

10.0%, at most 11%, at most 12%, at most 13%, at most 14%, at most 15%, at most 20%, at most

25%, or at most 30% by weight of the composition, or about 0.1% to about 0.5%, about 0.1% to about

0.75%, about 0.1% to about 1.0%, about 0.1% to about 1.5%, about 0.1% to about 2.0%, about 0.1%

to about 2.5%, about 0.1% to about 3.0%, about 0.1% to about 3.5%, about 0.1% to about 4.0%, about

0.1% to about 4.5%, about 0.1% to about 5.0%, about 0.2% to about 0.5%, about 0.2% to about 0.75%,

about 0.2% to about 1.0%, about 0.2% to about 1.5%, about 0.2% to about 2.0%, about 0.2% to about

2.5%, about 0.2% to about 3.0%, about 0.2% to about 3.5%, about 0.2% to about 4.0%, about 0.2% to

about 4.5%, about 0.2% to about 5.0%, about 0.5% to about 1.0%, about 0.5% to about 1.5%, about

0.5% to about 2.0%, about 0.5% to about 2.5%, about 0.5% to about 3.0%, about 0.5% to about 4.0%,

about 0.5% to about 5.0%, about 0.5% to about 6.0%, about 0.5% to about 7.0%, about 0.5% to about

8.0%, about 0.5% to about 9.0%, about 0.5% to about 10.0%, about 1.0% to about 2.5%, about 1.0%

to about 3.0%, about 1.0% to about 4.0%, about 1.0% to about 5.0%, about 1.0% to about 6.0%, about

1.0% to about 7.0%, about 1.0% to about 7.5%, about 1.0% to about 8.0%, about 1.0% to about 9.0%,

about 1.0% to about 10.0%, about 1.0% to about 15.0%, about 1.0% to about 20.0%, about 1.0% to

about 25.0%, about 1.0% to about 30.0%, about 2.0% to about 2.5%, about 2.0% to about 3.0%, about

2.0% to about 4.0%, about 2.0% to about 5.0%, about 2.0% to about 6.0%, about 2.0% to about 7.0%,

about 2.0% to about 7.5%, about 2.0% to about 8.0%, about 2.0% to about 9.0%, about 2.0% to about

10.0%, about 2.0% to about 15.0%, about 2.0% to about 20.0%, about 2.0% to about 25.0%, about

2.0% to about 30.0%, about 5.0% to about 6.0%, about 5.0% to about 7.0%, about 5.0% to about 7.5%,

about 5.0% to about 8.0%, about 5.0% to about 9.0%, about 5.0% to about 10.0%, about 5.0% to about

11.0%, about 5.0% to about 12.0%, about 5.0% to about 13.0%, about 5.0% to about 14.0%, about

5.0% to about 15.0%, about 5.0% to about 20.0%, about 5.0% to about 25.0%, about 5.0% to about

30.0%, about 10.0% to about 15.0%, about 10.0% to about 20.0%, about 10.0% to about 25.0%, about

10.0% to about 30.0%, about 15.0% to about 20.0%, about 15.0% to about 25.0%, about 15.0% to

about 30.0%, about 20.0% to about 25.0%, about 20.0% to about 30.0%, or about 20.0% to about

30.0%, by weight of the composition.

23. The composition according to any one of embodiments 6-8 or 14-22, wherein the one or more alcohols

include methanol, ethanol, propanol, isopropanol, butanol, pentanol, and/or 1-hexadecanol.

24. The composition according any one of embodiments 6-8 or 14-23, wherein the one or more alcohols

are in an amount of about 0.5%, about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about

7%, about 8%, about 9%, about 10%, about 11%, about 12%, about 13%, about 14%, about 15%,

about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about

60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, about 96%,

about 97%, about 98%, or about 99%, by weight of the composition, or at least 0.5%, at least 1%, at

least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9%, at

least 10%, at least 11%, at least 12%, at least 13%, at least 14%, at least 15%, at least 20%, at least

25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%,

at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least

96%, at least 97%, at least 98%, or at least 99%, by weight of the composition, or at most 0.5%, at

most 1%, at most 2%, at most 3%, at most 4%, at most 5%, at most 6%, at most 7%, at most 8%, at

most 9%, at most 10%, at most 11%, at most 12%, at most 13%, at most 14%, at most 15%, at most

20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%,

at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90%, at

most 95%, at most 96%, at most 97%, at most 98%, or at most 99%, by weight of the composition,

about 0.05% to about 0.75%, about 0.05% to about 1.0%, about 0.05% to about 1.5%, about 0.05% to

about 2.0%, about 0.05% to about 2.5%, about 0.1% to about 0.5%, about 0.1% to about 0.75%, about

about 0.1% to about 3.0%, about 0.1% to about 4.0%, about 0.1% to about 5.0%, about 0.2% to about

0.5%, about 0.2% to about 0.75%, about 0.2% to about 1.0%, about 0.2% to about 1.5%, about 0.2%

to about 2.0%, about 0.2% to about 2.5%, about 0.2% to about 3.0%, about 0.2% to about 4.0%, about

about 9.0%, about 0.5% to about 10.0%, about 1.0% to about 2.5%, about 1.0% to about 3.0%, about

10.0%, about 2.0% to about 2.5%, about 2.0% to about 3.0%, about 2.0% to about 4.0%, about 2.0%

to about 5.0%, about 2.0% to about 6.0%, about 2.0% to about 7.0%, about 2.0% to about 7.5%, about

2.0% to about 8.0%, about 2.0% to about 9.0%, about 2.0% to about 10.0%, about 5.0% to about 6.0%,

9.0%, about 5.0% to about 10.0%, about 5.0% to about 11.0%, about 5.0% to about 12.0%, about 5.0%

to about 13.0%, about 5.0% to about 14.0% or about 5.0% to about 15.0% by weight of the composition,

or about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about

50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about

90%, about 10% to about 95%, about 10% to about 97%, about 10% to about 99%, about 20% to about

30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about

70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 20% to about

97%, about 20% to about 99%, about 30% to about 40%, about 30% to about 50%, about 30% to about

60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about

95%, about 30% to about 97%, about 30% to about 99%, about 40% to about 50%, about 40% to about

60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about

95%, about 40% to about 97%, about 40% to about 99%, about 50% to about 60%, about 50% to about

70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 50% to about

97%, about 50% to about 99%, about 60% to about 70%, about 60% to about 80%, about 60% to about

90%, about 60% to about 95%, about 60% to about 97%, about 60% to about 99%, about 70% to about

80%, about 70% to about 90%, about 70% to about 95%, about 70% to about 97%, about 70% to about

99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 97%, about 80% to about

99%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, by weight of the

composition.

25. The composition according to any one of embodiments 9, 11, or 13-24, wherein the one or more metallic

particles include a metal acetate particle, a metal chloride particle, a metal nitrate particle, or a metal

oxide particle, or any combination thereof.

WO wo 2020/252433 PCT/US2020/037665 125

26. The composition according to any one of embodiments 9, 11 or 13-25, wherein the one or more metallic

particles are in an amount of 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm,

0.40 ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80 ppm, 0.85

ppm, 0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm,

45 ppm, 50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm, 95 ppm, 100

ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm, 350

ppm, 375 ppm, 400 ppm, 425 ppm, 450 ppm, 475 ppm, 500 ppm, 525 ppm, 550 ppm, 575 ppm, 600

ppm, 625 ppm, 650 ppm, 675 ppm, 700 ppm, 725 ppm, 750 ppm, 775 ppm, 800 ppm, 825 ppm, 850

ppm, 875 ppm, 900 ppm, 925 ppm, 950 ppm, 975 ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm, 1075 ppm,

1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm, 1,200 ppm, 1,225 ppm, 1,250 ppm, 1,275 ppm, 1,300

ppm, 1,325 ppm, 1,350 ppm, 1,375 ppm, 1,400 ppm, 1,425 ppm, 1,450 ppm, 1,475 ppm, 1,500 ppm,

1,600 ppm, 1,700 ppm, 1,800 ppm, 1,900 ppm, or 2,000 ppm or at least 0.05 ppm, at least 0.10 ppm,

at least 0.20 ppm, at least 0.30 ppm, at least 0.40 ppm, at least 0.50 ppm, at least 0.60 ppm, at least

0.70 ppm, at least 0.80 ppm, at least 0.90 ppm, at least 1 ppm, at least 10 ppm, at least 20 ppm, at

least 30 ppm, at least 40 ppm, at least 50 ppm, at least 60 ppm, at least 70 ppm, at least 80 ppm, at

least 90 ppm, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least 175 ppm, at least 200 ppm,

at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at least 325 ppm, at least 350

ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450 ppm, at least 475 ppm, at least

500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least 600 ppm, at least 625 ppm, at

least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at least 750 ppm, at least 775

ppm, at least 800 ppm, at least 825 ppm, at least 850 ppm, at least 875 ppm, at least 900 ppm, at least

925 ppm, at least 950 ppm, at least 975 ppm, at least 1,000 ppm, at least 1,025 ppm, at least 1,050

ppm, at least 1075 ppm, at least 1,100 ppm, at least 1,125 ppm, at least 1,150 ppm, at least 1,175

ppm, at least 1,200 ppm, at least 1,225 ppm, at least 1,250 ppm, at least 1,275 ppm, at least 1,300

ppm, at least 1,325 ppm, at least 1,350 ppm, at least 1,375 ppm, at least 1,400 ppm, at least 1,425

ppm, at least 1,450 ppm, at least 1,475 ppm, at least 1,500 ppm, at least 1,600 ppm, at least 1,700

ppm, at least 1,800 ppm, at least 1,900 ppm, or at least 2,000 ppm or at most 0.05 ppm, at most 0.10

ppm, at most 0.20 ppm, at most 0.30 ppm, at most 0.40 ppm, at most 0.50 ppm, at most 0.60 ppm, at

most 0.70 ppm, at most 0.80 ppm, at most 0.90 ppm, at most 1 ppm, at most 10 ppm, at most 20 ppm,

at most 30 ppm, at most 40 ppm, at most 50 ppm, at most 60 ppm, at most 70 ppm, at most 80 ppm,

at most 90 ppm, at most 100 ppm, at most 125 ppm, at most 150 ppm, at most 175 ppm, at most 200

ppm, at most 225 ppm, at most 250 ppm, at most 275 ppm, at most 300 ppm, at most 325 ppm, at most

350 ppm, at most 375 ppm, at most 400 ppm, at most 425 ppm, at most 450 ppm, at most 475 ppm, at

most 500 ppm, at most 525 ppm, at most 550 ppm, at most 575 ppm, at most 600 ppm, at most 625

ppm, at most 650 ppm, at most 675 ppm, at most 700 ppm, at most 725 ppm, at most 750 ppm, at most

775 ppm, at most 800 ppm, at most 825 ppm, at most 850 ppm, at most 875 ppm, at most 900 ppm, at

most 925 ppm, at most 950 ppm, at most 975 ppm, at most 1,000 ppm, at most 1,025 ppm, at most

1,050 ppm, at most 1075 ppm, at most 1,100 ppm, at most 1,125 ppm, at most 1,150 ppm, at most

1,175 ppm, at most 1,200 ppm, at most 1,225 ppm, at most 1,250 ppm, at most 1,275 ppm, at most

1,300 ppm, at most 1,325 ppm, at most 1,350 ppm, at most 1,375 ppm, at most 1,400 ppm, at most

1,425 ppm, at most 1,450 ppm, at most 1,475 ppm, at most 1,500 ppm, at most 1,600 ppm, at most

1,700 ppm, at most 1,800 ppm, at most 1,900 ppm, or at most 2,000 ppm or about 0.5 ppm to about

20 ppm, about 0.5 ppm to about 25 ppm, about 0.5 ppm to about 30 ppm, about 0.5 ppm to about 35

ppm, about 0.5 ppm to about 40 ppm, about 0.5 ppm to about 45 ppm, about 0.5 ppm to about 50 ppm,

about 0.5 ppm to about 55 ppm, about 0.5 ppm to about 60 ppm, about 0.5 ppm to about 65 ppm, about

0.5 ppm to about 70 ppm, about 0.5 ppm to about 75 ppm, about 0.5 ppm to about 80 ppm, about 0.5

ppm to about 85 ppm, about 0.5 ppm to about 90 ppm, about 0.5 ppm to about 95 ppm, about 0.5 ppm

to about 100 ppm, about 0.75 ppm to about 20 ppm, about 0.75 ppm to about 25 ppm, about 0.75 ppm

to about 30 ppm, about 0.75 ppm to about 35 ppm, about 0.75 ppm to about 40 ppm, about 0.75 ppm

to about 45 ppm, about 0.75 ppm to about 50 ppm, about 0.75 ppm to about 55 ppm, about 0.75 ppm

to about 60 ppm, about 0.75 ppm to about 65 ppm, about 0.75 ppm to about 70 ppm, about 0.75 ppm

to about 75 ppm, about 0.75 ppm to about 80 ppm, about 0.75 ppm to about 85 ppm, about 0.75 ppm

to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75 ppm to about 100 ppm, about 1 ppm to

about 20 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 30 ppm, about 1 ppm to about 35

ppm, about 1 ppm to about 40 ppm, about 1 ppm to about 45 ppm, about 1 ppm to about 50 ppm, about

1 ppm to about 55 ppm, about 1 ppm to about 60 ppm, about 1 ppm to about 65 ppm, about 1 ppm to

about 70 ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 80 ppm, about 1 ppm to about 85

ppm, about 1 ppm to about 90 ppm, about 1 ppm to about 95 ppm, about 1 ppm to about 100 ppm,

about 5 ppm to about 20 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 30 ppm, about 5

ppm to about 35 ppm, about 5 ppm to about 40 ppm, about 5 ppm to about 45 ppm, about 5 ppm to

ppm, about 350 ppm to about 500 ppm, about 350 ppm to about 525 ppm, about 350 ppm to about 550

ppm, about 350 ppm to about 575 ppm, about 350 ppm to about 600 ppm, about 400 ppm to about 500

ppm, about 400 ppm to about 600 ppm, about 400 ppm to about 700 ppm, about 400 ppm to about 800

ppm, about 400 ppm to about 900 ppm, about 400 ppm to about 1,000 ppm, about 400 ppm to about

PCT/US2020/037665 128

ppm, about 1,300 ppm to about 1,500 ppm, about 1,300 ppm to about 1,600 ppm, about 1,300 ppm to

about 1,700 ppm, about 1,300 ppm to about 1,800 ppm, about 1,300 ppm to about 1,900 ppm, about

ppm, about 1,500 ppm to about 2,000 ppm, about 1,600 ppm to about 1,700 ppm, about 1,600 ppm to about 1,800 ppm, about 1,600 ppm to about 1,900 ppm, about 1,600 ppm to about 2,000 ppm, about

to about 2,000 ppm.

27. The composition according to any one of embodiments 9, 11, or 13-25, wherein the one or more metallic

particles are in an amount of about 0.001%, about 0.005%, about 0.01%, about 0.05%, about 0.10%,

about 0.11%, about 0.12%, about 0.13%, about 0.14%, about 0.15%, about 0.16%, about 0.17%, about

0.18%, about 0.19%, about 0.2%, about 0.25%, about 0.3%, about 0.35%, about 0.4%, about 0.45%,

or about 0.5% by weight of the composition, or at least 0.001%, at least 0.005%, at least 0.01%, at

least 0.05%, at least 0.10%, at least 0.11%, at least 0.12%, at least 0.13%, at least 0.14%, at least

0.15%, at least 0.16%, at least 0.17%, at least 0.18%, at least 0.19%, at least 0.2%, at least 0.25%, at

least 0.3%, at least 0.35%, at least 0.4%, at least 0.45%, or at least 0.5% by weight of the composition,

or at most 0.001%, at most 0.005%, at most 0.01%, at most 0.05%, at most 0.10%, at most 0.11%, at

most 0.12%, at most 0.13%, at most 0.14%, at most 0.15%, at most 0.16%, at most 0.17%, at most

0.18%, at most 0.19%, at most 0.2%, at most 0.25%, at most 0.3%, at most 0.35%, at most 0.4%, at

most 0.45%, or at most 0.5% by weight of the composition, or about 0.0010% to about 0.005%, about

0.0010% to about 0.010%, about 0.0010% to about 0.050%, about 0.0010% to about 0.10%, about

0.0010% to about 0.15%, about 0.0010% to about 0.20%, about 0.0010% to about 0.25%, about

0.0010% to about 0.30%, about 0.0010% to about 0.35%, about 0.0010% to about 0.40%, about

of the composition.

28. The composition according to any one of embodiments 9, 11, or 13-27, wherein the one or more metallic

particles have a mean diameter of about 10 nm, about 20 nm, about 30 nm, about 40 nm, about 50 nm,

about 60 nm, about 70 nm, about 80 nm, about 90 nm, about 100 nm, or at least 10 nm, at least 20

nm, at least 30 nm, at least 40 nm, at least 50 nm, at least 60 nm, at least 70 nm, at least 80 nm, at

least 90 nm, at least 100 nm, or at most 10 nm, at most 20 nm, at most 30 nm, at most 40 nm, at most

50 nm, at most 60 nm, at most 70 nm, at most 80 nm, at most 90 nm, at most 100 nm, or about 10 nm

to about 20 nm, about 10 nm to about 30 nm, about 10 nm to about 40 nm, about 10 nm to about 50

nm, about 10 nm to about 60 nm, about 10 nm to about 70 nm, about 10 nm to about 80 nm, about 10

nm to about 90 nm, about 10 nm to about 100 nm, about 20 nm to about 30 nm, about 20 nm to about

40 nm, about 20 nm to about 50 nm, about 20 nm to about 60 nm, about 20 nm to about 70 nm, about

20 nm to about 80 nm, about 20 nm to about 90 nm, about 20 nm to about 100 nm, about 30 nm to

PCT/US2020/037665 130 130

about 40 nm, about 30 nm to about 50 nm, about 30 nm to about 60 nm, about 30 nm to about 70 nm,

about 30 nm to about 80 nm, about 30 nm to about 90 nm, about 30 nm to about 100 nm, about 40 nm

to about 50 nm, about 40 nm to about 60 nm, about 40 nm to about 70 nm, about 40 nm to about 80

nm, about 40 nm to about 90 nm, about 40 nm to about 100 nm, about 50 nm to about 60 nm, about

50 nm to about 70 nm, about 50 nm to about 80 nm, about 50 nm to about 90 nm, about 50 nm to about

100 nm, about 60 nm to about 70 nm, about 60 nm to about 80 nm, about 60 nm to about 90 nm, about

60 nm to about 100 nm, about 70 nm to about 80 nm, about 70 nm to about 90 nm, about 70 nm to

about 100 nm, about 80 nm to about 90 nm, about 80 nm to about 100 nm, or about 90 nm to about

100 nm.

29. The composition according to any one of embodiments 10 or 12-28, wherein the one or more metal

salts include copper chloride, iron chloride, titanium chloride, zinc chloride, silver nitrate, or any

combination thereof.

30. The composition according to any one of embodiments 10 or 12-29, wherein the one or more metal

salt are in an amount of 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm,

PCT/US2020/037665 131

at most 30 ppm, at most 40 ppm,atmost50 ppm,atmost 60 ppm,atmost 70ppm,atmost 80 ppm, at most 90 ppm, at most 100 ppm, at most 125 ppm, at most 150 ppm, at most 175 ppm, at most 200

WO wo 2020/252433 PCT/US2020/037665 132

ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 300 ppm to about 425 ppm, about 300 ppm to about 450 ppm, about 300 ppm to about 475 ppm, about 300 ppm to about 500 ppm, about 300 ppm to about 525 ppm, about 300 ppm to about 550 ppm, about 300 ppm to about 575 ppm, about 300 ppm to about 600 ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, about 375 ppm to about 400 ppm, about 350 ppm to about 425 ppm, about 350 ppm to about 450 ppm, about 350 ppm to about 475 ppm, about 350 ppm to about 500 ppm, about 350 ppm to about 525 ppm, about 350 ppm to about 550 ppm, about 350 ppm to about 575 ppm, about 350 ppm to about 600 ppm, about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, about 400 ppm to about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about 400 ppm to about 1,000 ppm, about 400 ppm to about

ppm, about 1,100 ppm to about 1,400 ppm, about 1,100 ppm to about 1,500 ppm, about 1,100 ppm to about 1,600 ppm, about 1,100 ppm to about 1,700 ppm, about 1,100 ppm to about 1,800 ppm, about

1,100 ppm to about 1,900 ppm, about ,100 ppm to about 2,000 ppm, about 1,200 ppm to about 1,300

to about 2,000 ppm.

31. The composition according to any one of embodiments 10 or 12-30, wherein the one or more metal

salt are in an amount of about 0.001%, about 0.005%, about 0.01%, about 0.05%, about 0.10%, about

0.11%, about 0.12%, about 0.13%, about 0.14%, about 0.15%, about 0.16%, about 0.17%, about

WO wo 2020/252433 PCT/US2020/037665 135

of the composition.

32. The composition according to any one of embodiments 1-31, further comprising, consisting essentially

of, or consisting of one or more carriers.

33. The composition according to embodiment 32, wherein the one or more carrier includes an aqueous

carrier, a semi-solid carrier, a solid carrier, or any combination thereof.

34. The composition according to embodiment 32 or 33, wherein the one or more carrier includes water, a

vegetable oil, a mineral oil, an ester oil, an ether, an alcohol, a fatty alcohol, an isoparaffin, a

hydrocarbon oil, a polyol, a wax, or any combination thereof.

35. The composition according to embodiment 34, wherein the ester oil includes octal palmitate, isopropyl

myristate or isopropyl palmitate.

36. The composition according to embodiment 34, wherein the ether includes dicapryl ether or dimethyl

isosorbide.

37. The composition according to embodiment 34, wherein the alcohol includes ethanol or isopropanol.

38. The composition according to embodiment 34, wherein the fatty alcohol includes cetyl alcohol, cetearyl

alcohol, stearyl alcohol or behenyl alcohol.

39. The composition according to embodiment 34, wherein the isoparaffin includes isooctane, isododecane

(IDD) or isohexadecane.

40. The composition according to embodiment 34, wherein the hydrocarbon oil includes mineral oil,

petrolatum, isoeicosane or a polyolefin.

41. The composition according to embodiment 34, wherein the polyol includes propylene glycol, glycerin,

butylene glycol, pentylene glycol, hexylene glycol, or caprylyl glycol.

42. The composition according to embodiment 34, wherein the wax includes beeswax, carnauba, ozokerite,

microcrystalline wax, polyethylene wax, or a botanical wax.

43. The composition according to any one of embodiments 32-42, wherein the one or more carriers are in

an amount of at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least

35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%,

98% or at least 99% by weight of the composition or at most 5%, at most 10%, at most 15%, at most

most 95%, at most 96%, at most 97%, at most 98% or at most 99% by weight of the composition or

about 5% to about 25%, about 5% to about 50%, about 5% to about 75%, about 5% to about 90%,

about 5% to about 95%, about 5% to about 96%, about 5% to about 97%, about 5% to about 98%,

about 5% to about 99%, about 25% to about 50%, about 25% to about 75%, about 25% to about 90%,

about 25% to about 95%, about 25% to about 96%, about 25% to about 97%, about 25% to about 98%,

about 25% to about 99%, about 50% to about 75%, about 50% to about 90%, about 50% to about 95%,

about 50% to about 96%, about 50% to about 97%, about 50% to about 98%, about 50% to about 99%,

about 75% to about 80%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%,

about 75% to about 96%, about 75% to about 97%, about 75% to about 98%, about 75% to about 99%,

about 80% to about 85%, about 80% to about 90%, about 80% to about 95%, about 80% to about 96%, about 80% to about 97%, about 80% to about 98%, about 80% to about 99%, about 85% to about 90%, about 85% to about 95%, about 85% to about 96%, about 85% to about 97%, about 85% to about 98%, about 85% to about 99%, about 90% to about 95%, about 90% to about 96%, about 90% to about 97%, about 90% to about 98%, about 90% to about 99%, or about 95% to about 99%, by weight of the composition.

44. The composition according to any one of embodiments 1-43, further comprising, consisting essentially

of, or consisting of one or more additional ingredients.

45. The composition according to embodiment 44, wherein the one or more additional ingredient includes

a preservative, a chelating agent, or any combination thereof.

46. The composition according to any one of embodiments 1-45, having a pH of about 2 to about 5, about

2 to about 5.5, about 2 to about 6, about 2 to about 6.5, about 2 to about 7, about 2 to about 7.5, about

2 to about 8, about 2 to about 8.5, about 2 to about 9, about 2.5 to about 5, about 2.5 to about 5.5,

about 2.5 to about 6, about 2.5 to about 6.5, about 2.5 to about 7, about 2.5 to about 7.5, about 2.5 to

about 8, about 2.5 to about 8.5, about 2.5 to about 9, about 3 to about 5, about 3 to about 5.5, about 3

to about 6, about 3 to about 6.5, about 3 to about 7, about 3 to about 7.5, about 3 to about 8, about 3

to about 8.5, about 3 to about 9, about 3.5 to about 5, about 3.5 to about 5.5, about 3.5 to about 6,

about 3.5 to about 6.5, about 3.5 to about 7, about 3.5 to about 7.5, about 3.5 to about 8, about 3.5 to

about 8.5, about 3.5 to about 9, about 4 to about 5, about 4 to about 5.5, about 4 to about 6, about 4 to

about 6.5, about 4 to about 7, about 4 to about 7.5, about 4 to about 8, about 4 to about 8.5, about 4 to

about 9, about 4.5 to about 5, about 4.5 to about 5.5, about 4.5 to about 6, about 4.5 to about 6.5, about

4.5 to about 7, about 4.5 to about 7.5, about 4.5 to about 8, about 4.5 to about 8.5, about 4.5 to about

9, about 5 to about 5.5, about 5 to about 6, about 5 to about 6.5, about 5 to about 7, about 5 to about

7.5, about 5 to about 8, about 5 to about 8.5, about 5 to about 9, about 5.5 to about 6, about 5.5 to

about 6.5, about 5.5 to about 7, about 5.5 to about 7.5, about 5.5 to about 8, about 5.5 to about 8.5,

about 5.5 to about 9, about 6 to about 6.5, about 6 to about 7, about 6 to about 7.5, about 6 to about 8,

about 6 to about 8.5, about 6 to about 9, about 6.5 to about 7, about 6.5 to about 7.5, about 6.5 to about

8, about 6.5 to about 8.5, about 6.5 to about 9, about 7 to about 7.5, about 7 to about 8, about 7 to

about 8.5, about 7 to about 9, about 7.5 to about 8, about 7.5 to about 8.5, about 7.5 to about 9, about

8 to about 8.5, about 8 to about 9, or about 8.5 to about 9.

47. A kit comprising, consisting essentially of, or consisting of a composition as defined in any one of

embodiments 1-46.

48. The kit according to embodiment 47, further comprising, consisting essentially of, or consisting of one

or more delivery or application systems, and/or instructions, and/or a container.

49. A kit comprising, consisting essentially of, or consisting of a first component including hypochlorous

acid or free available chlorine.

50. A kit comprising, consisting essentially of, or consisting of a first component including hypochlorous

acid or free available chlorine and a second component including or more quaternary compounds or

silicon quaternary compounds.

51. A kit comprising, consisting essentially of, or consisting of a first component including hypochlorous

acid or free available chlorine and a second component including one or more guanide-containing

compounds.

PCT/US2020/037665 137

52. A kit comprising, consisting essentially of, or consisting of a first component including one or more

alcohols and a second component including one or more quaternary compounds or silicon quaternary

compounds. 53. A kit comprising, consisting essentially of, or consisting of a first component including one or more

alcohols and a second component including one or more guanide-containing compounds.

54. A kit comprising, consisting essentially of, or consisting of a first component including hypochlorous

acid or free available chlorine and a second component including one or more metallic particles.

55. A kit comprising, consisting essentially of, or consisting of a first component including hypochlorous

acid or free available chlorine and a second component including one or more metal salts.

56. A kit comprising, consisting essentially of, or consisting of a first component including hypochlorous

acid or free available chlorine, a second component including one or more metallic particles and a third

component including one or more metal salts.

57. The kit according to any one of embodiments 50-56, further comprising, consisting essentially of, or

consisting of a third component including one or more metallic particles and/or a fourth component

including one or more metal salts, and/or a fifth component including one or more cationic surfactants,

and/or a sixth component including a rinse solution and/or one or more delivery or application systems,

and/or instructions, and/or a container

58. The kit according to embodiments 47-51 and 54-56, wherein the hypochlorous acid or free available

chlorine is in an amount of 0.01 ppm, 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm,

0.35 ppm, 0.40 ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80

ppm, 0.85 ppm, 0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35

ppm, 40 ppm, 45 ppm, 50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm,

95 ppm, 100 ppm, 125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325

ppm, 350 ppm, 375 ppm, 400 ppm, 425 ppm, 450 ppm, 475 ppm, 500 ppm, 525 ppm, 550 ppm, 575

ppm, 600 ppm, 625 ppm, 650 ppm, 675 ppm, 700 ppm, 725 ppm, 750 ppm, 775 ppm, 800 ppm, 825

ppm, 850 ppm, 875 ppm, 900 ppm, 925 ppm, 950 ppm, 975 ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm,

1075 ppm, 1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm, 1,200 ppm, 1,225 ppm, 1,250 ppm, 1,275

ppm, 1,300 ppm, 1,325 ppm, 1,350 ppm, 1,375 ppm, 1,400 ppm, 1,425 ppm, 1,450 ppm, 1,475 ppm,

1,500 ppm, 1,600 ppm, 1,700 ppm, 1,800 ppm, 1,900 ppm, or 2,000 ppm or at least 0.01 ppm, at least

0.05 ppm, at least 0.10 ppm, at least 0.20 ppm, at least 0.30 ppm, at least 0.40 ppm, at least 0.50 ppm,

at least 0.60 ppm, at least 0.70 ppm, at least 0.80 ppm, at least 0.90 ppm, at least 1 ppm, at least 10

ppm, at least 20 ppm, at least 30 ppm, at least 40 ppm, at least 50 ppm, at least 60 ppm, at least 70

ppm, at least 80 ppm, at least 90 ppm, at least 100 ppm, at least 125 ppm, at least 150 ppm, at least

175 ppm, at least 200 ppm, at least 225 ppm, at least 250 ppm, at least 275 ppm, at least 300 ppm, at

least 325 ppm, at least 350 ppm, at least 375 ppm, at least 400 ppm, at least 425 ppm, at least 450

ppm, at least 475 ppm, at least 500 ppm, at least 525 ppm, at least 550 ppm, at least 575 ppm, at least

600 ppm, at least 625 ppm, at least 650 ppm, at least 675 ppm, at least 700 ppm, at least 725 ppm, at

least 750 ppm, at least 775 ppm, at least 800 ppm, at least 825 ppm, at least 850 ppm, at least 875

ppm, at least 900 ppm, at least 925 ppm, at least 950 ppm, at least 975 ppm, at least 1,000 ppm, at

least 1,025 ppm, at least 1,050 ppm, at least 1075 ppm, at least 1,100 ppm, at least 1,125 ppm, at least

1,150 ppm, at least 1,175 ppm, at least 1,200 ppm, at least 1,225 ppm, at least 1,250 ppm, at least

PCT/US2020/037665 138

1,275 ppm, at least 1,300 ppm, at least 1,325 ppm, at least 1,350 ppm, at least 1,375 ppm, at least

1,400 ppm, at least 1,425 ppm, at least 1,450 ppm, at least 1,475 ppm, at least 1,500 ppm, at least

1,600 ppm, at least 1,700 ppm, at least 1,800 ppm, at least 1,900 ppm, or at least 2,000 ppm or at most

0.01 ppm, at most 0.05 ppm, at most 0.10 ppm, at most 0.20 ppm, at most 0.30 ppm, at most 0.40

ppm, at most 0.50 ppm, at most 0.60 ppm, at most 0.70 ppm, at most 0.80 ppm, at most 0.90 ppm, at

most 1 ppm, at most 10 ppm, at most 20 ppm, at most 30 ppm, at most 40 ppm, at most 50 ppm, at

most 60 ppm, at most 70 ppm, at most 80 ppm, at most 90 ppm, at most 100 ppm, at most 125 ppm,

at most 150 ppm, at most 175 ppm, at most 200 ppm, at most 225 ppm, at most 250 ppm, at most 275

ppm, at most 300 ppm, at most 325 ppm, at most 350 ppm, at most 375 ppm, at most 400 ppm, at most

425 ppm, at most 450 ppm, at most 475 ppm, at most 500 ppm, at most 525 ppm, at most 550 ppm, at

most 575 ppm, at most 600 ppm, at most 625 ppm, at most 650 ppm, at most 675 ppm, at most 700

most 1,000 ppm, at most 1,025 ppm, at most 1,050 ppm, at most 1075 ppm, at most 1,100 ppm, at

most 1,125 ppm, at most 1,150 ppm, at most 1,175 ppm, at most 1,200 ppm, at most 1,225 ppm, at

most 1,250 ppm, at most 1,275 ppm, at most 1,300 ppm, at most 1,325 ppm, at most 1,350 ppm, at

most 1,375 ppm, at most 1,400 ppm, at most 1,425 ppm, at most 1,450 ppm, at most 1,475 ppm, at

most 1,500 ppm, at most 1,600 ppm, at most 1,700 ppm, at most 1,800 ppm, at most 1,900 ppm, or at

most 2,000 ppm or at most 0.05 ppm, about 0.5 ppm to about 55 ppm, about 0.5 ppm to about 60 ppm,

0.5 ppm to about 80 ppm, about 0.5 ppm to about 85 ppm, about 0.5 ppm to about 90 ppm, about 0.5

ppm to about 95 ppm, about 0.5 ppm to about 100 ppm, about 0.75 ppm to about 20 ppm, about 0.75

ppm to about 25 ppm, about 0.75 ppm to about 30 ppm, about 0.75 ppm to about 35 ppm, about 0.75

ppm to about 40 ppm, about 0.75 ppm to about 45 ppm, about 0.75 ppm to about 50 ppm, about 0.75

ppm to about 55 ppm, about 0.75 ppm to about 60 ppm, about 0.75 ppm to about 65 ppm, about 0.75

ppm to about 70 ppm, about 0.75 ppm to about 75 ppm, about 0.75 ppm to about 80 ppm, about 0.75

ppm to about 85 ppm, about 0.75 ppm to about 90 ppm, about 0.75 ppm to about 95 ppm, about 0.75

ppm to about 100 ppm, about 1 ppm to about 20 ppm, about 1 ppm to about 25 ppm, about 1 ppm to

about 30 ppm, about 1 ppm to about 35 ppm, about 1 ppm to about 40 ppm, about 1 ppm to about 45

ppm, about 1 ppm to about 50 ppm, about 1 ppm to about 55 ppm, about 1 ppm to about 60 ppm, about

1 ppm to about 65 ppm, about 1 ppm to about 70 ppm, about 1 ppm to about 75 ppm, about 1 ppm to

about 80 ppm, about 1 ppm to about 85 ppm, about 1 ppm to about 90 ppm, about 1 ppm to about 95

ppm, about 1 ppm to about 100 ppm, about 5 ppm to about 20 ppm, about 5 ppm to about 25 ppm,

about 5 ppm to about 30 ppm, about 5 ppm to about 35 ppm, about 5 ppm to about 40 ppm, about 5

ppm to about 45 ppm, about 5 ppm to about 50 ppm, about 5 ppm to about 55 ppm, about 5 ppm to

about 60 ppm, about 5 ppm to about 65 ppm, about 5 ppm to about 70 ppm, about 5 ppm to about 75

ppm, about 5 ppm to about 80 ppm, about 5 ppm to about 85 ppm, about 5 ppm to about 90 ppm, about

5 ppm to about 95 ppm, about 5 ppm to about 100 ppm, about 10 ppm to about 20 ppm, about 10 ppm

to about 25 ppm, about 10 ppm to about 30 ppm, about 10 ppm to about 35 ppm, about 10 ppm to

about 40 ppm, about 10 ppm to about 45 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about

55 ppm, about 10 ppm to about 60 ppm, about 10 ppm to about 65 ppm, about 10 ppm to about 70 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 80 ppm, about 10 ppm to about 85 ppm, about 10 ppm to about 90 ppm, about 10 ppm to about 95 ppm, or about 10 ppm to about 100 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 50 ppm, about 1 ppm to about 75 ppm, about 1 ppm to about 100 ppm, about 1 ppm to about 125 ppm, about 1 ppm to about 150 ppm, about 1 ppm to about 175 ppm, about 1 ppm to about 200 ppm, about 1 ppm to about 225 ppm, about 1 ppm to about 250 ppm, about 1 ppm to about 275 ppm, about 1 ppm to about 300 ppm, about 1 ppm to about

325 ppm, about 1 ppm to about 350 ppm, about 1 ppm to about 375 ppm, about 1 ppm to about 400

ppm, about 1 ppm to about 425 ppm, about 1 ppm to about 450 ppm, about 1 ppm to about 475 ppm,

about 1 ppm to about 500 ppm, about 5 ppm to about 25 ppm, about 5 ppm to about 50 ppm, about 5

ppm to about 75 ppm, about 5 ppm to about 100 ppm, about 5 ppm to about 125 ppm, about 5 ppm to

about 150 ppm, about 5 ppm to about 175 ppm, about 5 ppm to about 200 ppm, about 5 ppm to about

225 ppm, about 5 ppm to about 250 ppm, about 5 ppm to about 275 ppm, about 1 ppm to about 300

ppm, about 5 ppm to about 325 ppm, about 5 ppm to about 350 ppm, about 5 ppm to about 375 ppm,

about 5 ppm to about 400 ppm, about 5 ppm to about 425 ppm, about 5 ppm to about 450 ppm, about

5 ppm to about 475 ppm, about 5 ppm to about 500 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 75 ppm, about 10 ppm to about 100 ppm, about 10 ppm

about 350 ppm, about 10 ppm to about 375 ppm, about 10 ppm to about 400 ppm, about 10 ppm to

about 425 ppm, about 10 ppm to about 450 ppm, about 10 ppm to about 475 ppm, about 10 ppm to

about 500 ppm, about 25 ppm to about 50 ppm, about 25 ppm to about 75 ppm, about 25 ppm to about

100 ppm, about 25 ppm to about 125 ppm, about 25 ppm to about 150 ppm, about 25 ppm to about

175 ppm, about 25 ppm to about 200 ppm, about 25 ppm to about 225 ppm, about 25 ppm to about

250 ppm, about 25 ppm to about 275 ppm, about 25 ppm to about 300 ppm, about 25 ppm to about

325 ppm, about 25 ppm to about 350 ppm, about 25 ppm to about 375 ppm, about 25 ppm to about

400 ppm, about 25 ppm to about 425 ppm, about 25 ppm to about 450 ppm, about 25 ppm to about

475 ppm, about 25 ppm to about 500 ppm, about 50 ppm to about 75 ppm, about 50 ppm to about 100

ppm, about 50 ppm to about 125 ppm, about 50 ppm to about 150 ppm, about 50 ppm to about 175

ppm, about 50 ppm to about 200 ppm, about 50 ppm to about 225 ppm, about 50 ppm to about 250

ppm, about 50 ppm to about 275 ppm, about 50 ppm to about 300 ppm, about 50 ppm to about 325

ppm, about 50 ppm to about 350 ppm, about 50 ppm to about 375 ppm, about 50 ppm to about 400

ppm, about 50 ppm to about 425 ppm, about 50 ppm to about 450 ppm, about 50 ppm to about 475

ppm, about 50 ppm to about 500 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125

ppm, about 75 ppm to about 375 ppm, about 75 ppm to about 400 ppm, about 75 ppm to about 425

ppm, about 75 ppm to about 450 ppm, about 75 ppm to about 475 ppm, about 75 ppm to about 500

ppm, about 100 ppm to about 125 ppm, about 100 ppm to about 150 ppm, about 100 ppm to about 175

ppm, about 100 ppm to about 200 ppm, about 100 ppm to about 225 ppm, about 100 ppm to about 250

WO wo 2020/252433 PCT/US2020/037665 140

ppm, about 100 ppm to about 275 ppm, about 100 ppm to about 300 ppm, about 100 ppm to about 325

ppm, about 100 ppm to about 350 ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400

ppm, about 150 ppm to about 175 ppm, about 150 ppm to about 200 ppm, about 150 ppm to about 225

ppm, about 150 ppm to about 250 ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300

ppm, about 150 ppm to about 325 ppm, about 150 ppm to about 350 ppm, about 150 ppm to about 375

ppm, about 150 ppm to about 400 ppm, about 200 ppm to about 225 ppm, about 200 ppm to about 250

ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 325

ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400

ppm, about 200 ppm to about 425 ppm, about 200 ppm to about 450 ppm, about 200 ppm to about 475

ppm, about 200 ppm to about 500 ppm, about 200 ppm to about 525 ppm, about 200 ppm to about 550

ppm, about 200 ppm to about 575 ppm, about 200 ppm to about 600 ppm, about 250 ppm to about 275

ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400 ppm, about 250 ppm to about 425

ppm, about 250 ppm to about 450 ppm, about 250 ppm to about 475 ppm, about 250 ppm to about 500

ppm, about 250 ppm to about 525 ppm, about 250 ppm to about 550 ppm, about 250 ppm to about 575

ppm, about 250 ppm to about 600 ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350

ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 300 ppm to about 425

ppm, about 300 ppm to about 450 ppm, about 300 ppm to about 475 ppm, about 300 ppm to about 500

ppm, about 300 ppm to about 525 ppm, about 300 ppm to about 550 ppm, about 300 ppm to about 575

ppm, about 300 ppm to about 600 ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400

ppm, about 375 ppm to about 400 ppm, about 350 ppm to about 425 ppm, about 350 ppm to about 450

ppm, about 350 ppm to about 475 ppm, about 350 ppm to about 500 ppm, about 350 ppm to about 525

ppm, about 350 ppm to about 550 ppm, about 350 ppm to about 575 ppm, about 350 ppm to about 600

about 1,300 ppm, about 400 ppm to about 1,400 ppm, about 400 ppm to about 1,500 ppm, about 400

ppm to about 1,600 ppm, about 400 ppm to about 1,700 ppm, about 400 ppm to about 1,800 ppm,

about 400 ppm to about 1,900 ppm, about 400 ppm to about 2,000 ppm, about 500 ppm to about 600

ppm, about 500 ppm to about 700 ppm, about 500 ppm to about 800 ppm, about 500 ppm to about 900

ppm, about 500 ppm to about 1,000 ppm, about 500 ppm to about 1,100 ppm, about 500 ppm to about

1,200 ppm, about 500 ppm to about 1,300 ppm, about 500 ppm to about 1,400 ppm, about 500 ppm to

about 1,500 ppm, about 500 ppm to about 1,600 ppm, about 500 ppm to about 1,700 ppm, about 500

ppm to about 1,800 ppm, about 500 ppm to about 1,900 ppm, about 500 ppm to about 2,000 ppm,

about 1,800 ppm, about 600 ppm to about 1,900 ppm, about 600 ppm to about 2,000 ppm, about 700

ppm to about 800 ppm, about 700 ppm to about 900 ppm, about 700 ppm to about 1,000 ppm, about

700 ppm to about 1,100 ppm, about 700 ppm to about 1,200 ppm, about 700 ppm to about 1,300 ppm, about 700 ppm to about 1,400 ppm, about 700 ppm to about 1,500 ppm, about 700 ppm to about 1,600 ppm, about 700 ppm to about 1,700 ppm, about 700 ppm to about 1,800 ppm, about 700 ppm to about

1,900 ppm, about 700 ppm to about 2,000 ppm, about 800 ppm to about 900 ppm, about 800 ppm to

about 1,000 ppm, about 800 ppm to about 1,100 ppm, about 800 ppm to about 1,200 ppm, about 800

ppm to about 1,300 ppm, about 800 ppm to about 1,400 ppm, about 800 ppm to about 1,500 ppm,

about 800 ppm to about 1,600 ppm, about 800 ppm to about 1,700 ppm, about 800 ppm to about 1,800

ppm, about 800 ppm to about 1,900 ppm, about 800 ppm to about 2,000 ppm, about 900 ppm to about

1,000 ppm, about 900 ppm to about 1,100 ppm, about 900 ppm to about 1,200 ppm, about 900 ppm to

about 1,300 ppm, about 900 ppm to about 1,400 ppm, about 900 ppm to about 1,500 ppm, about 900

ppm to about 1,600 ppm, about 900 ppm to about 1,700 ppm, about 900 ppm to about 1,800 ppm,

about 900 ppm to about 1,900 ppm, about 900 ppm to about 2,000 ppm, about 1,000 ppm to about

1,100 ppm, about 1,000 ppm to about 1,200 ppm, about 1,000 ppm to about 1,300 ppm, about 1,000

ppm to about 1,400 ppm, about 1,000 ppm to about 1,500 ppm, about 1,000 ppm to about 1,600 ppm,

about 1,000 ppm to about 1,700 ppm, about 1,000 ppm to about 1,800 ppm, about 1,000 ppm to about

1,900 ppm, about 1,000 ppm to about 2,000 ppm, about 1,100 ppm to about 1,200 ppm, about 1,100

ppm to about 1,300 ppm, about 1,100 ppm to about 1,400 ppm, about 1,100 ppm to about 1,500 ppm,

about 1,100 ppm to about 1,600 ppm, about 1,100 ppm to about 1,700 ppm, about 1,100 ppm to about

1,800 ppm, about 1,100 ppm to about 1,900 ppm, about 1,100 ppm to about 2,000 ppm, about 1,200

ppm to about 1,300 ppm, about 1,200 ppm to about 1,400 ppm, about 1,200 ppm to about 1,500 ppm,

about 1,200 ppm to about 1,600 ppm, about 1,200 ppm to about 1,700 ppm, about 1,200 ppm to about

1,800 ppm, about 1,200 ppm to about 1,900 ppm, about 1,200 ppm to about 2,000 ppm, about 1,300

ppm to about 1,400 ppm, about 1,300 ppm to about 1,500 ppm, about 1,300 ppm to about 1,600 ppm,

about 1,300 ppm to about 1,700 ppm, about 1,300 ppm to about 1,800 ppm, about 1,300 ppm to about

1,900 ppm, about 1,300 ppm to about 2,000 ppm, about 1,400 ppm to about 1,500 ppm, about 1,400

ppm to about 1,600 ppm, about 1,400 ppm to about 1,700 ppm, about 1,400 ppm to about 1,800 ppm,

about 1,400 ppm to about 1,900 ppm, about 1,400 ppm to about 2,000 ppm, about 1,500 ppm to about

1,600 ppm, about 1,500 ppm to about 1,700 ppm, about 1,500 ppm to about 1,800 ppm, about 1,500

ppm to about 1,900 ppm, about 1,500 ppm to about 2,000 ppm, about 1,600 ppm to about 1,700 ppm,

about 1,600 ppm to about 1,800 ppm, about 1,600 ppm to about 1,900 ppm, about 1,600 ppm to about

2,000 ppm, about 1,700 ppm to about 1,800 ppm, about 1,700 ppm to about 1,900 ppm, about 1,700

ppm to about 2,000 ppm, about 1,800 ppm to about 1,900 ppm, about 1,800 ppm to about 2,000 ppm,

or about 1,900 ppm to about 2,000 ppm.

59. The kit according to any one of embodiments 47-51 and 54-56, wherein the hypochlorous acid or free

available chlorine is in an amount of about 0.001%, about 0.005%, about 0.01%, about 0.05%, about

0.10%, about 0.11%, about 0.12%, about 0.13%, about 0.14%, about 0.15%, about 0.16%, about

composition, or at most 0.001%, at most 0.005%, at most 0.01%, at most 0.05%, at most 0.10%, at most 0.11%, at most 0.12%, at most 0.13%, at most 0.14%, at most 0.15%, at most 0.16%, at most

of the composition.

60. The kit according to any one of embodiments 47-51, 52 or 56, wherein the one or more quaternary

compounds include one or more dialkyl quaternary compounds and/or one or more polyether fatty

quaternary compounds and/or wherein the one or more silicon quaternary compounds include one or

more silicone dialkyl quaternary compounds and/or one or more silicone polyether fatty quaternary

compounds. 61. The kit according to embodiment 60, wherein the one or more dialkyl quaternary compounds include

didodecyldimethylammonium chloride and di-n-alkyldimethyl ammonium chloride and/or wherein the

one or more silicone dialkyl quaternary compounds include dimethyloctadecyl[3- (trimethoxysilyl)propyl]ammonium chloride and tetradecyldimethyl-(3-trimethoxysilyl)propyl ammonium

chloride.

62. The kit according to any one of embodiments 47-51, 52, 56, 60 or 61, wherein the one or more

most 1.5%, at most 2.0%, at most 2.5%, at most 3.0%, at most 4.0%, at most 5.0%, at most 6.0%, at most 7.5%, at most 8.0%, at most 9.0%, at most 10.0%, at most 11.0%, at most 12.0%, at most 13.0%, at most 14.0%, at most 15.0%, at most 16.0%, at most 17.0%, at most 18.0%, at most 19.0%, or at most 20.0% by weight of the composition by weight of the composition, or about 0.1% to about 0.5%, about 0.1% to about 0.75%, about 0.1% to about 1.0%, about 0.1% to about 1.5%, about 0.1% to about

15%, about 5.0% to about 6.0%, about 5.0% to about 7.0%, about 5.0% to about 8.0%, about 5.0% to

about 9.0%, about 5.0% to about 10%, about 5.0% to about 11%, about 5.0% to about 12%, about

13% to about 15%, or 14% to about 15% by weight of the composition.

63. The kit according to any one of embodiments 47-51, 52, 56, 60 or 61, wherein the one or more

ppm, 20 ppm, 25 ppm,30ppm,35ppm,40ppm,45ppm,50ppm,55ppm,60 ppm,65ppm,70ppm,

ppm, 750 ppm,775ppm,800 ppm,825ppm,850ppm,875ppm,900ppm,925ppm,950 ppm, 975 ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm, 1075 ppm, 1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm,

0.20 ppm, at most 0.30 ppm, at most 0.40 ppm, at most 0.50 ppm, at most 0.60 ppm, at most 0.70

ppm, at most 0.80 ppm, at most 0.90 ppm, at most 1 ppm, at most 10 ppm, at most 20 ppm, at most

about 375 ppm, about 1 ppm to about 400 ppm, about 1 ppm to about 425 ppm, about 1 ppm to about

450 ppm, about 1 ppm to about 475 ppm, about 1 ppm to about 500 ppm, about 5 ppm to about 25

ppm, about 5 ppm to about 50 ppm, about 5 ppm to about 75 ppm, about 5 ppm to about 100 ppm,

about 5 ppm to about 125 ppm, about 5 ppm to about 150 ppm, about 5 ppm to about 175 ppm, about

5 ppm to about 200 ppm, about 5 ppm to about 225 ppm, about 5 ppm to about 250 ppm, about 5 ppm

to about 275 ppm, about 1 ppm to about 300 ppm, about 5 ppm to about 325 ppm, about 5 ppm to

about 350 ppm, about 5 ppm to about 375 ppm, about 5 ppm to about 400 ppm, about 5 ppm to about

425 ppm, about 5 ppm to about 450 ppm, about 5 ppm to about 475 ppm, about 5 ppm to about 500

ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 75 ppm,

about 10 ppm to about 100 ppm, about 10 ppm to about 125 ppm, about 10 ppm to about 150 ppm,

about 10 ppm to about 175 ppm, about 10 ppm to about 200 ppm, about 10 ppm to about 225 ppm,

about 10 ppm to about 250 ppm, about 10 ppm to about 275 ppm, about 10 ppm to about 300 ppm,

about 10 ppm to about 325 ppm, about 10 ppm to about 350 ppm, about 10 ppm to about 375 ppm,

about 10 ppm to about 400 ppm, about 10 ppm to about 425 ppm, about 10 ppm to about 450 ppm,

about 10 ppm to about 475 ppm, about 10 ppm to about 500 ppm, about 25 ppm to about 50 ppm,

about 25 ppm to about 75 ppm, about 25 ppm to about 100 ppm, about 25 ppm to about 125 ppm, about 25 ppm to about 150 ppm, about 25 ppm to about 175 ppm, about 25 ppm to about 200 ppm, about 25 ppm to about 225 ppm, about 25 ppm to about 250 ppm, about 25 ppm to about 275 ppm, about 25 ppm to about 300 ppm, about 25 ppm to about 325 ppm, about 25 ppm to about 350 ppm, about 25 ppm to about 375 ppm, about 25 ppm to about 400 ppm, about 25 ppm to about 425 ppm, about 25 ppm to about 450 ppm, about 25 ppm to about 475 ppm, about 25 ppm to about 500 ppm, about 50 ppm to about 75 ppm, about 50 ppm to about 100 ppm, about 50 ppm to about 125 ppm, about 50 ppm to about 150 ppm, about 50 ppm to about 175 ppm, about 50 ppm to about 200 ppm, about 50 ppm to about 225 ppm, about 50 ppm to about 250 ppm, about 50 ppm to about 275 ppm, about 50 ppm to about 300 ppm, about 50 ppm to about 325 ppm, about 50 ppm to about 350 ppm, about 50 ppm to about 375 ppm, about 50 ppm to about 400 ppm, about 50 ppm to about 425 ppm, about 50 ppm to about 450 ppm, about 50 ppm to about 475 ppm, about 50 ppm to about 500 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125 ppm, about 75 ppm to about 150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200 ppm, about 75 ppm to about 225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, about 75 ppm to about 300 ppm, about 75 ppm to about 325 ppm, about 75 ppm to about 350 ppm, about 75 ppm to about 375 ppm, about 75 ppm to about 400 ppm, about 75 ppm to about 425 ppm, about 75 ppm to about 450 ppm, about 75 ppm to about 475 ppm, about 75 ppm to about 500 ppm, about 100 ppm to about 125 ppm, about 100 ppm to about 150 ppm, about 100 ppm to about 175 ppm, about 100 ppm to about 200 ppm, about 100 ppm to about 225 ppm, about 100 ppm to about 250 ppm, about 100 ppm to about 275 ppm, about 100 ppm to about 300 ppm, about 100 ppm to about 325 ppm, about 100 ppm to about 350 ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400 ppm, about 150 ppm to about 175 ppm, about 150 ppm to about 200 ppm, about 150 ppm to about 225 ppm, about 150 ppm to about 250 ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300 ppm, about 150 ppm to about 325 ppm, about 150 ppm to about 350 ppm, about 150 ppm to about 375 ppm, about 150 ppm to about 400 ppm, about 200 ppm to about 225 ppm, about 200 ppm to about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400 ppm, about 200 ppm to about 425 ppm, about 200 ppm to about 450 ppm, about 200 ppm to about 475 ppm, about 200 ppm to about 500 ppm, about 200 ppm to about 525 ppm, about 200 ppm to about 550 ppm, about 200 ppm to about 575 ppm, about 200 ppm to about 600 ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300 ppm, about 250 ppm to about 325 ppm, about 250 ppm to about 350 ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400 ppm, about 250 ppm to about 425 ppm, about 250 ppm to about 450 ppm, about 250 ppm to about 475 ppm, about 250 ppm to about 500 ppm, about 250 ppm to about 525 ppm, about 250 ppm to about 550 ppm, about 250 ppm to about 575 ppm, about 250 ppm to about 600 ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 300 ppm to about 425 ppm, about 300 ppm to about 450 ppm, about 300 ppm to about 475 ppm, about 300 ppm to about 500 ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, about 350 ppm to about 425 ppm, about 350 ppm to about 450 ppm, about 350 ppm to about 475 ppm, about 350 ppm to about 500 ppm, about 400 ppm to about 425 ppm, about 400 ppm to about 450 ppm, about 400 ppm to about 475 ppm, about 400 ppm to about 500 ppm, about 450 ppm to about 475 ppm, or about 450 ppm to about 500 ppm, about 400 ppm to about 600

WO wo 2020/252433 PCT/US2020/037665 147

ppm, about 400 ppm to about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900

ppm, about 400 ppm to about 1,000 ppm, about 400 ppm to about 1,100 ppm, about 400 ppm to about

1,200 ppm, about 400 ppm to about 1,300 ppm, about 400 ppm to about 1,400 ppm, about 400 ppm to

about 1,500 ppm, about 500 ppm to about 600 ppm, about 500 ppm to about 700 ppm, about 500 ppm

to about 800 ppm, about 500 ppm to about 900 ppm, about 500 ppm to about 1,000 ppm, about 500

ppm to about 1,100 ppm, about 500 ppm to about 1,200 ppm, about 500 ppm to about 1,300 ppm,

about 500 ppm to about 1,400 ppm, about 500 ppm to about 1,500 ppm, about 600 ppm to about 700

about 1,300 ppm, about 600 ppm to about 1,400 ppm, about 600 ppm to about 1,500 ppm, about 700

700 ppm to about 1,100 ppm, about 700 ppm to about 1,200 ppm, about 700 ppm to about 1,300 ppm,

about 700 ppm to about 1,400 ppm, about 700 ppm to about 1,500 ppm, about 800 ppm to about 900

ppm, about 800 ppm to about 1,000 ppm, about 800 ppm to about 1,100 ppm, about 800 ppm to about

1,200 ppm, about 800 ppm to about 1,300 ppm, about 800 ppm to about 1,400 ppm, about 800 ppm to

about 1,500 ppm, about 900 ppm to about 1,000 ppm, about 900 ppm to about 1,100 ppm, about 900

ppm to about 1,200 ppm, about 900 ppm to about 1,300 ppm, about 900 ppm to about 1,400 ppm,

about 900 ppm to about 1,500 ppm, about 1,000 ppm to about 1,100 ppm, about 1,000 ppm to about

1,200 ppm, about 1,000 ppm to about 1,300 ppm, about 1,000 ppm to about 1,400 ppm, about 1,000

ppm to about 1,500 ppm, about 1,100 ppm to about 1,200 ppm, about 1,100 ppm to about 1,300 ppm,

about 1,100 ppm to about 1,400 ppm, about 1,100 ppm to about 1,500 ppm, about 1,200 ppm to about

1,300 ppm, about 1,200 ppm to about 1,400 ppm, about 1,200 ppm to about 1,500 ppm, about 1,300

ppm to about 1,400 ppm, about 1,300 ppm to about 1,500 ppm, or about 1,400 ppm to about 1,500

ppm. 64. The kit according to any one of embodiments 47, 48, 51, 53 or 57, wherein the one or more guanide-

containing compounds comprise, consist essentially of, or consist of a biguanide, a biguanide-

containing compound, or any combination thereof.

65. The kit according to embodiment 64, wherein the one or more biguanide-containing compounds include

a polyhexamethylene biguanide (PHMB), a polyaminopropyl biguanide (PAPB), a 1,1'-(1,6- Hexanediyl)bis{2-[N'-(2-ethylhexyl)carbamimidoyl]guanidine) (alexidine), a chlorhexidine, a chlorhexidine gluconate, or any combination thereof.

66. The kit according to any one of embodiments 47, 48, 51, 53, 57, 64 or 65, wherein the one or more

WO wo 2020/252433 PCT/US2020/037665 148

30.0%, by weight of the composition.

67. The kit according to any one of embodiments 47, 48, 51, 53 or 57, wherein the one or more alcohols

68. The kit according to any one of embodiments 47, 48, 51, 53, 57 or 67, wherein the one or more alcohols

WO wo 2020/252433 PCT/US2020/037665 PCT/US2020/037665 149

PCT/US2020/037665 150

composition.

69. The kit according to any one of embodiments 47, 48, 54, 56, or 57, wherein the one or more metallic

oxide particle, or any combination thereof.

70. The kit according to any one of embodiments 47, 48, 54, 56, 57 or 69, wherein the one or more metallic

775 ppm, at most 800 ppm, at most 825 ppm, at most 850 ppm, at most 875 ppm, at most 900 ppm, at most 925 ppm, at most 950 ppm, at most 975 ppm, at most 1,000 ppm, at most 1,025 ppm, at most

1 ppm to about 375 ppm, about 1 ppm to about 400 ppm, about 1 ppm to about 425 ppm, about 1 ppm

to about 450 ppm, about 1 ppm to about 475 ppm, about 1 ppm to about 500 ppm, about 5 ppm to

about 25 ppm, about 5 ppm to about 50 ppm, about 5 ppm to about 75 ppm, about 5 ppm to about 100

ppm, about 5 ppm to about 125 ppm, about 5 ppm to about 150 ppm, about 5 ppm to about 175 ppm,

WO wo 2020/252433 PCT/US2020/037665 152

about 5 ppm to about 200 ppm, about 5 ppm to about 225 ppm, about 5 ppm to about 250 ppm, about

5 ppm to about 275 ppm, about 1 ppm to about 300 ppm, about 5 ppm to about 325 ppm, about 5 ppm

to about 350 ppm, about 5 ppm to about 375 ppm, about 5 ppm to about 400 ppm, about 5 ppm to

about 425 ppm, about 5 ppm to about 450 ppm, about 5 ppm to about 475 ppm, about 5 ppm to about

500 ppm, about 10 ppm to about 25 ppm, about 10 ppm to about 50 ppm, about 10 ppm to about 75

ppm, about 10 ppm to about 100 ppm, about 10 ppm to about 125 ppm, about 10 ppm to about 150

ppm, about 10 ppm to about 175 ppm, about 10 ppm to about 200 ppm, about 10 ppm to about 225

ppm, about 10 ppm to about 250 ppm, about 10 ppm to about 275 ppm, about 10 ppm to about 300

ppm, about 10 ppm to about 325 ppm, about 10 ppm to about 350 ppm, about 10 ppm to about 375

ppm, about 10 ppm to about 400 ppm, about 10 ppm to about 425 ppm, about 10 ppm to about 450

ppm, about 10 ppm to about 475 ppm, about 10 ppm to about 500 ppm, about 25 ppm to about 50

ppm, about 25 ppm to about 375 ppm, about 25 ppm to about 400 ppm, about 25 ppm to about 425

ppm, about 25 ppm to about 450 ppm, about 25 ppm to about 475 ppm, about 25 ppm to about 500

ppm, about 50 ppm to about 75 ppm, about 50 ppm to about 100 ppm, about 50 ppm to about 125

ppm, about 50 ppm to about 150 ppm, about 50 ppm to about 175 ppm, about 50 ppm to about 200

ppm, about 50 ppm to about 225 ppm, about 50 ppm to about 250 ppm, about 50 ppm to about 275

ppm, about 50 ppm to about 300 ppm, about 50 ppm to about 325 ppm, about 50 ppm to about 350

ppm, about 50 ppm to about 375 ppm, about 50 ppm to about 400 ppm, about 50 ppm to about 425

ppm, about 50 ppm to about 450 ppm, about 50 ppm to about 475 ppm, about 50 ppm to about 500

ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125 ppm, about 75 ppm to about 150

ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200 ppm, about 75 ppm to about 225

ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, about 75 ppm to about 300

ppm, about 75 ppm to about 325 ppm, about 75 ppm to about 350 ppm, about 75 ppm to about 375

ppm, about 75 ppm to about 400 ppm, about 75 ppm to about 425 ppm, about 75 ppm to about 450

ppm, about 75 ppm to about 475 ppm, about 75 ppm to about 500 ppm, about 100 ppm to about 125

ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400 ppm, about 100 ppm to about 425

ppm, about 100 ppm to about 450 ppm, about 100 ppm to about 475 ppm, about 100 ppm to about 500

ppm, about 150 ppm to about 400 ppm, about 150 ppm to about 425 ppm, about 150 ppm to about 450

ppm, about 150 ppm to about 475 ppm, about 150 ppm to about 500 ppm, about 200 ppm to about 225

ppm, about 200 ppm to about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300

ppm, about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375

PCT/US2020/037665 153 153

ppm, about 200 ppm to about 400 ppm, about 200 ppm to about 425 ppm, about 200 ppm to about 450

ppm, about 200 ppm to about 475 ppm, about 200 ppm to about 500 ppm, about 200 ppm to about 525

ppm, about 200 ppm to about 550 ppm, about 200 ppm to about 575 ppm, about 200 ppm to about 600

ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300 ppm, about 250 ppm to about 325

ppm, about 250 ppm to about 350 ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400

ppm, about 250 ppm to about 425 ppm, about 250 ppm to about 450 ppm, about 250 ppm to about 475

ppm, about 250 ppm to about 500 ppm, about 250 ppm to about 525 ppm, about 250 ppm to about 550

ppm, about 250 ppm to about 575 ppm, about 250 ppm to about 600 ppm, about 300 ppm to about 325

ppm, about 300 ppm to about 425 ppm, about 300 ppm to about 450 ppm, about 300 ppm to about 475

ppm, about 300 ppm to about 500 ppm, about 300 ppm to about 525 ppm, about 300 ppm to about 550

ppm, about 300 ppm to about 575 ppm, about 300 ppm to about 600 ppm, about 350 ppm to about 375

ppm, about 350 ppm to about 400 ppm, about 375 ppm to about 400 ppm, about 350 ppm to about 425

ppm, about 350 ppm to about 450 ppm, about 350 ppm to about 475 ppm, about 350 ppm to about 500

ppm, about 350 ppm to about 525 ppm, about 350 ppm to about 550 ppm, about 350 ppm to about 575

ppm, about 350 ppm to about 600 ppm, about 400 ppm to about 500 ppm, about 400 ppm to about 600

about 1,500 ppm, about 400 ppm to about 1,600 ppm, about 400 ppm to about 1,700 ppm, about 400

ppm to about 1,800 ppm, about 400 ppm to about 1,900 ppm, about 400 ppm to about 2,000 ppm,

about 500 ppm to about 600 ppm, about 500 ppm to about 700 ppm, about 500 ppm to about 800 ppm,

about 500 ppm to about 900 ppm, about 500 ppm to about 1,000 ppm, about 500 ppm to about ,100

ppm, about 500 ppm to about 1,200 ppm, about 500 ppm to about 1,300 ppm, about 500 ppm to about

1,400 ppm, about 500 ppm to about 1,500 ppm, about 500 ppm to about 1,600 ppm, about 500 ppm to

about 1,700 ppm, about 500 ppm to about 1,800 ppm, about 500 ppm to about 1,900 ppm, about 500

ppm to about 2,000 ppm, about 600 ppm to about 700 ppm, about 600 ppm to about 800 ppm, about

600 ppm to about 900 ppm, about 600 ppm to about 1,000 ppm, about 600 ppm to about 1,100 ppm,

about 600 ppm to about 1,200 ppm, about 600 ppm to about 1,300 ppm, about 600 ppm to about 1,400

ppm, about 600 ppm to about 1,500 ppm, about 600 ppm to about 1,600 ppm, about 600 ppm to about

1,700 ppm, about 600 ppm to about 1,800 ppm, about 600 ppm to about 1,900 ppm, about 600 ppm to

about 2,000 ppm, about 700 ppm to about 800 ppm, about 700 ppm to about 900 ppm, about 700 ppm

to about 1,000 ppm, about 700 ppm to about 1,100 ppm, about 700 ppm to about 1,200 ppm, about

700 ppm to about 1,300 ppm, about 700 ppm to about 1,400 ppm, about 700 ppm to about 1,500 ppm,

about 700 ppm to about 1,600 ppm, about 700 ppm to about 1,700 ppm, about 700 ppm to about 1,800

ppm, about 700 ppm to about 1,900 ppm, about 700 ppm to about 2,000 ppm, about 800 ppm to about

900 ppm, about 800 ppm to about 1,000 ppm, about 800 ppm to about 1,100 ppm, about 800 ppm to

about 1,200 ppm, about 800 ppm to about 1,300 ppm, about 800 ppm to about 1,400 ppm, about 800

ppm to about 1,500 ppm, about 800 ppm to about 1,600 ppm, about 800 ppm to about 1,700 ppm,

about 800 ppm to about 1,800 ppm, about 800 ppm to about 1,900 ppm, about 800 ppm to about 2,000

ppm, about 900 ppm to about 1,000 ppm, about 900 ppm to about 1,100 ppm, about 900 ppm to about

WO wo 2020/252433 PCT/US2020/037665 154

1,200 ppm, about 900 ppm to about 1,300 ppm, about 900 ppm to about 1,400 ppm, about 900 ppm to

about 1,500 ppm, about 900 ppm to about 1,600 ppm, about 900 ppm to about 1,700 ppm, about 900

ppm to about 1,800 ppm, about 900 ppm to about 1,900 ppm, about 900 ppm to about 2,000 ppm,

1,300 ppm, about 1,000 ppm to about 1,400 ppm, about 1,000 ppm to about 1,500 ppm, about 1,000

ppm to about 1,600 ppm, about 1,000 ppm to about 1,700 ppm, about 1,000 ppm to about 1,800 ppm,

about 1,000 ppm to about 1,900 ppm, about 1,000 ppm to about 2,000 ppm, about 1,100 ppm to about

1,200 ppm, about 1,100 ppm to about 1,300 ppm, about 1,100 ppm to about 1,400 ppm, about 1,100

2,000 ppm, about 1,200 ppm to about 1,300 ppm, about 1,200 ppm to about 1,400 ppm, about 1,200

ppm to about 1,500 ppm, about 1,200 ppm to about 1,600 ppm, about 1,200 ppm to about 1,700 ppm,

about 1,200 ppm to about 1,800 ppm, about 1,200 ppm to about 1,900 ppm, about 1,200 ppm to about

2,000 ppm, about 1,300 ppm to about 1,400 ppm, about 1,300 ppm to about 1,500 ppm, about 1,300

ppm to about 1,600 ppm, about 1,300 ppm to about 1,700 ppm, about 1,300 ppm to about 1,800 ppm,

about 1,300 ppm to about 1,900 ppm, about 1,300 ppm to about 2,000 ppm, about 1,400 ppm to about

1,500 ppm, about 1,400 ppm to about 1,600 ppm, about 1,400 ppm to about 1,700 ppm, about 1,400

ppm to about 1,800 ppm, about 1,400 ppm to about 1,900 ppm, about 1,400 ppm to about 2,000 ppm,

about 1,500 ppm to about 1,600 ppm, about 1,500 ppm to about 1,700 ppm, about 1,500 ppm to about

1,800 ppm, about 1,500 ppm to about 1,900 ppm, about 1,500 ppm to about 2,000 ppm, about 1,600

1,900 ppm, about 1,700 ppm to about 2,000 ppm, about 1,800 ppm to about 1,900 ppm, about 1,800

ppm to about 2,000 ppm, or about 1,900 ppm to about 2,000 ppm.

71. The kit according to any one of embodiments 47, 48, 54, 56, 57 or 69, wherein the one or more metallic

0.010% to about 0.10%, about 0.010% to about 0.15%, about 0.010% to about 0.20%, about 0.010% to about 0.25%, about 0.010% to about 0.30%, about 0.010% to about 0.35%, about 0.010% to about

of the composition.

72. The kit according to any one of embodiments 47, 48, 54, 56, 57 or 69-71, wherein the one or more

metallic particles have a mean diameter of about 10 nm, about 20 nm, about 30 nm, about 40 nm,

about 50 nm, about 60 nm, about 70 nm, about 80 nm, about 90 nm, about 100 nm, or at least 10 nm,

at least 20 nm, at least 30 nm, at least 40 nm, at least 50 nm, at least 60 nm, at least 70 nm, at least

80 nm, at least 90 nm, at least 100 nm, or at most 10 nm, at most 20 nm, at most 30 nm, at most 40

nm, at most 50 nm, at most 60 nm, at most 70 nm, at most 80 nm, at most 90 nm, at most 100 nm, or

about 10 nm to about 20 nm, about 10 nm to about 30 nm, about 10 nm to about 40 nm, about 10 nm

to about 50 nm, about 10 nm to about 60 nm, about 10 nm to about 70 nm, about 10 nm to about 80

nm, about 10 nm to about 90 nm, about 10 nm to about 100 nm, about 20 nm to about 30 nm, about

20 nm to about 40 nm, about 20 nm to about 50 nm, about 20 nm to about 60 nm, about 20 nm to about

70 nm, about 20 nm to about 80 nm, about 20 nm to about 90 nm, about 20 nm to about 100 nm, about

30 nm to about 40 nm, about 30 nm to about 50 nm, about 30 nm to about 60 nm, about 30 nm to about

70 nm, about 30 nm to about 80 nm, about 30 nm to about 90 nm, about 30 nm to about 100 nm, about

40 nm to about 50 nm, about 40 nm to about 60 nm, about 40 nm to about 70 nm, about 40 nm to about

80 nm, about 40 nm to about 90 nm, about 40 nm to about 100 nm, about 50 nm to about 60 nm, about

100 nm.

73. The kit according to any one of embodiments 47, 48, 56 or 57, wherein the one or more metal salts

include copper chloride, iron chloride, titanium chloride, zinc chloride, silver nitrate, or any combination

thereof.

74. The kit according to any one of embodiments 47, 48, 56, 57 or 73, wherein the one or more metal salts

are in an amount of 0.05 ppm, 0.10 ppm, 0.15 ppm, 0.20 ppm, 0.25 ppm, 0.30 ppm, 0.35 ppm, 0.40

ppm, 0.45 ppm, 0.50 ppm, 0.55 ppm, 0.60 ppm, 0.65 ppm, 0.70 ppm, 0.75 ppm, 0.80 ppm, 0.85 ppm,

0.90 ppm, 0.95 ppm, 1 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, 35 ppm, 40 ppm, 45

ppm, 50 ppm, 55 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 85 ppm, 90 ppm, 95 ppm, 100 ppm,

125 ppm, 150 ppm, 175 ppm, 200 ppm, 225 ppm, 250 ppm, 275 ppm, 300 ppm, 325 ppm, 350 ppm,

375 ppm, 400 ppm, 425 ppm, 450 ppm, 475 ppm, 500 ppm, 525 ppm, 550 ppm, 575 ppm, 600 ppm,

625 ppm, 650 ppm, 675 ppm, 700 ppm, 725 ppm, 750 ppm, 775 ppm, 800 ppm, 825 ppm, 850 ppm,

875 ppm, 900 ppm, 925 ppm, 950 ppm, 975 ppm, 1,000 ppm, 1,025 ppm, 1,050 ppm, 1075 ppm, 1,100 ppm, 1,125 ppm, 1,150 ppm, 1,175 ppm, 1,200 ppm, 1,225 ppm, 1,250 ppm, 1,275 ppm, 1,300 ppm,

1,325 ppm, 1,350 ppm, 1,375 ppm, 1,400 ppm, 1,425 ppm, 1,450 ppm, 1,475 ppm, 1,500 ppm, 1,600

ppm, 1,700 ppm, 1,800 ppm, 1,900 ppm, or 2,000 ppm or at least 0.05 ppm, at least 0.10 ppm, at least

ppm, at least 675 ppm, at least 700ppm,atleast 725ppm,atleast 750ppm,atleast 775ppm, at least

1,450 ppm, at least 1,475 ppm, at least 1,500 ppm, at least 1,600 ppm, at least 1,700 ppm, at least

1,800 ppm, at least 1,900 ppm, or at least 2,000 ppm or at most 0.05 ppm, at most 0.10 ppm, at most

ppm, at most 1,450 ppm, at most 1,475 ppm, at most 1,500 ppm, at most 1,600 ppm, at most 1,700

ppm, at most 1,800 ppm, at most 1,900 ppm, or at most 2,000 ppm or about 0.5 ppm to about 20 ppm,

WO wo 2020/252433 PCT/US2020/037665 157

95 ppm, or about 10 ppm to about 100 ppm, about 1 ppm to about 25 ppm, about 1 ppm to about 50

about 25 ppm to about 75 ppm, about 25 ppm to about 100 ppm, about 25 ppm to about 125 ppm,

about 25 ppm to about 150 ppm, about 25 ppm to about 175 ppm, about 25 ppm to about 200 ppm,

about 25 ppm to about 225 ppm, about 25 ppm to about 250 ppm, about 25 ppm to about 275 ppm, about 25 ppm to about 300 ppm, about 25 ppm to about 325 ppm, about 25 ppm to about 350 ppm, about 25 ppm to about 375 ppm, about 25 ppm to about 400 ppm, about 25 ppm to about 425 ppm, about 25 ppm to about 450 ppm, about 25 ppm to about 475 ppm, about 25 ppm to about 500 ppm, about 50 ppm to about 75 ppm, about 50 ppm to about 100 ppm, about 50 ppm to about 125 ppm, about 50 ppm to about 150 ppm, about 50 ppm to about 175 ppm, about 50 ppm to about 200 ppm, about 50 ppm to about 225 ppm, about 50 ppm to about 250 ppm, about 50 ppm to about 275 ppm, about 50 ppm to about 300 ppm, about 50 ppm to about 325 ppm, about 50 ppm to about 350 ppm, about 50 ppm to about 375 ppm, about 50 ppm to about 400 ppm, about 50 ppm to about 425 ppm, about 50 ppm to about 450 ppm, about 50 ppm to about 475 ppm, about 50 ppm to about 500 ppm, about 75 ppm to about 100 ppm, about 75 ppm to about 125 ppm, about 75 ppm to about 150 ppm, about 75 ppm to about 175 ppm, about 75 ppm to about 200 ppm, about 75 ppm to about 225 ppm, about 75 ppm to about 250 ppm, about 75 ppm to about 275 ppm, about 75 ppm to about 300 ppm, about 75 ppm to about 325 ppm, about 75 ppm to about 350 ppm, about 75 ppm to about 375 ppm, about 75 ppm to about 400 ppm, about 75 ppm to about 425 ppm, about 75 ppm to about 450 ppm, about 75 ppm to about 475 ppm, about 75 ppm to about 500 ppm, about 100 ppm to about 125 ppm, about 100 ppm to about 150 ppm, about 100 ppm to about 175 ppm, about 100 ppm to about 200 ppm, about 100 ppm to about 225 ppm, about 100 ppm to about 250 ppm, about 100 ppm to about 275 ppm, about 100 ppm to about 300 ppm, about 100 ppm to about 325 ppm, about 100 ppm to about 350 ppm, about 100 ppm to about 375 ppm, about 100 ppm to about 400 ppm, about 100 ppm to about 425 ppm, about 100 ppm to about 450 ppm, about 100 ppm to about 475 ppm, about 100 ppm to about 500 ppm, about 150 ppm to about 175 ppm, about 150 ppm to about 200 ppm, about 150 ppm to about 225 ppm, about 150 ppm to about 250 ppm, about 150 ppm to about 275 ppm, about 150 ppm to about 300 ppm, about 150 ppm to about 325 ppm, about 150 ppm to about 350 ppm, about 150 ppm to about 375 ppm, about 150 ppm to about 400 ppm, about 150 ppm to about 425 ppm, about 150 ppm to about 450 ppm, about 150 ppm to about 475 ppm, about 150 ppm to about 500 ppm, about 200 ppm to about 225 ppm, about 200 ppm to about 250 ppm, about 200 ppm to about 275 ppm, about 200 ppm to about 300 ppm, about 200 ppm to about 325 ppm, about 200 ppm to about 350 ppm, about 200 ppm to about 375 ppm, about 200 ppm to about 400 ppm, about 200 ppm to about 425 ppm, about 200 ppm to about 450 ppm, about 200 ppm to about 475 ppm, about 200 ppm to about 500 ppm, about 200 ppm to about 525 ppm, about 200 ppm to about 550 ppm, about 200 ppm to about 575 ppm, about 200 ppm to about 600 ppm, about 250 ppm to about 275 ppm, about 250 ppm to about 300 ppm, about 250 ppm to about 325 ppm, about 250 ppm to about 350 ppm, about 250 ppm to about 375 ppm, about 250 ppm to about 400 ppm, about 250 ppm to about 425 ppm, about 250 ppm to about 450 ppm, about 250 ppm to about 475 ppm, about 250 ppm to about 500 ppm, about 250 ppm to about 525 ppm, about 250 ppm to about 550 ppm, about 250 ppm to about 575 ppm, about 250 ppm to about 600 ppm, about 300 ppm to about 325 ppm, about 300 ppm to about 350 ppm, about 300 ppm to about 375 ppm, about 300 ppm to about 400 ppm, about 300 ppm to about 425 ppm, about 300 ppm to about 450 ppm, about 300 ppm to about 475 ppm, about 300 ppm to about 500 ppm, about 300 ppm to about 525 ppm, about 300 ppm to about 550 ppm, about 300 ppm to about 575 ppm, about 300 ppm to about 600 ppm, about 350 ppm to about 375 ppm, about 350 ppm to about 400 ppm, about 375 ppm to about 400 ppm, about 350 ppm to about 425 ppm, about 350 ppm to about 450 ppm, about 350 ppm to about 475 ppm, about 350 ppm to about 500 ppm, about 350 ppm to about 525 ppm, about 350 ppm to about 550 ppm, about 350 ppm to about 575 ppm, about 350 ppm to about 600 ppm, about 400 ppm to about 500 ppm, about 400 ppm to about 600 ppm, about 400 ppm to about 700 ppm, about 400 ppm to about 800 ppm, about 400 ppm to about 900 ppm, about 400 ppm to about 1,000 ppm, about 400 ppm to about 1,100 ppm, about 400 ppm to about 1,200 ppm, about 400 ppm to about 1,300 ppm, about 400 ppm to about 1,400 ppm, about 400 ppm to about

1,500 ppm, about 400 ppm to about 1,600 ppm, about 400 ppm to about 1,700 ppm, about 400 ppm to

about 1,800 ppm, about 400 ppm to about 1,900 ppm, about 400 ppm to about 2,000 ppm, about 500

ppm to about 600 ppm, about 500 ppm to about 700 ppm, about 500 ppm to about 800 ppm, about 500

ppm to about 900 ppm, about 500 ppm to about 1,000 ppm, about 500 ppm to about 1,100 ppm, about

about 500 ppm to about 1,500 ppm, about 500 ppm to about 1,600 ppm, about 500 ppm to about 1,700

ppm, about 500 ppm to about 1,800 ppm, about 500 ppm to about 1,900 ppm, about 500 ppm to about

2,000 ppm, about 600 ppm to about 700 ppm, about 600 ppm to about 800 ppm, about 600 ppm to

about 900 ppm, about 600 ppm to about 1,000 ppm, about 600 ppm to about 1,100 ppm, about 600

ppm to about 1,200 ppm, about 600 ppm to about 1,300 ppm, about 600 ppm to about 1,400 ppm,

about 600 ppm to about 1,500 ppm, about 600 ppm to about 1,600 ppm, about 600 ppm to about 1,700

ppm, about 600 ppm to about 1,800 ppm, about 600 ppm to about 1,900 ppm, about 600 ppm to about

2,000 ppm, about 700 ppm to about 800 ppm, about 700 ppm to about 900 ppm, about 700 ppm to

about 1,000 ppm, about 700 ppm to about 1,100 ppm, about 700 ppm to about 1,200 ppm, about 700

ppm to about 1,300 ppm, about 700 ppm to about 1,400 ppm, about 700 ppm to about 1,500 ppm,

2,000 ppm, about 1,300 ppm to about 1,400 ppm, about 1,300 ppm to about 1,500 ppm, about 1,300 ppm to about 1,600 ppm, about 1,300 ppm to about 1,700 ppm, about 1,300 ppm to about 1,800 ppm, about 1,300 ppm to about 1,900 ppm, about 1,300 ppm to about 2,000 ppm, about 1,400 ppm to about

ppm to about 2,000 ppm, or about 1,900 ppm to about 2,000 ppm.

75. The kit according to any one of embodiments 47, 48, 56, 57 or 73, wherein the one or more metal salts

are in an amount of about 0.001%, about 0.005%, about 0.01%, about 0.05%, about 0.10%, about

of the composition.

76. A method of reducing or inhibiting microbial growth in water or other liquid, the method comprising,

consisting essentially of, or consisting of applying a composition as defined in any one of embodiments

1-46 or applying the components of a kit as defined in any one of embodiments 47-75, to the water or

the other liquid in a microbiostatic effective amount, wherein application of the composition reduces or

inhibits microbial growth.

77. A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in reducing or inhibiting microbial growth in water or

other liquid

78. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to reduce or inhibit microbial growth in water or other

liquid

79. A method of reducing or inhibiting microbial growth in a water reservoir of a medical device, the method

comprising, consisting essentially of, or consisting of applying a composition as defined in any one of

embodiments 1-46 or applying the components of a kit as defined in any one of embodiments 47-75,

to water or other liquid present in the water reservoir of the medical device in a microbiostatic effective

amount, wherein application of the composition reduces or inhibits microbial growth.

to a water reservoir of the medical device, wherein application of the composition reduces or inhibits

microbial growth prior and during operation of the medical device.

80. A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in reducing or inhibiting microbial growth in a water

reservoir of a medical device.

81. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to reduce or inhibit microbial growth in a water reservoir

of a medical device.

82. A method to wash or irrigate a surface area, the method comprising, consisting essentially of, or

consisting of applying a composition as defined in any one of embodiments 1-46 or applying the

components of a kit as defined in any one of embodiments 47-75 to the surface area, wherein

application of the composition washes or irrigates the surface area.

83. A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in washing or irrigating a surface area.

84. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to wash or irrigate a surface area.

85. The method of embodiment 82, or the use of embodiment 83 or 84, wherein the surface area is a

porous surface area or a non-porous surface area.

86. The method of embodiment 82 or 85, or the use of any one of embodiments 83-85, wherein the surface

area comprises a table top, counter top, floor, wall, ceiling, window, door, door handle, shower, bath,

sink, faucet, toilet, toilet seat, drain, equipment, machinery, personal protective gear, personal

biohazard gear, a medical device, dental device, pharmaceutical device, veterinary device, mortuary

device or human skin.

87. A method to wash or irrigate a wound in an individual, the method comprising, consisting essentially

of, or consisting of applying a composition as defined in any one of embodiments 1-46 or applying the

components of a kit as defined in any one of embodiments 47-75 to the wound, wherein application of

the composition washes or irrigates the wound.

88. A method to treat and/or provide relief from a wound in an individual, the method comprising, consisting

essentially of, or consisting of applying a composition as defined in any one of embodiments 1-46 or

applying the components of a kit as defined in any one of embodiments 47-75 to the wound, wherein

application of the composition treats and/or provides relief the wound.

89. A method to wash or irrigate an infection in an individual, the method comprising, consisting essentially

components of a kit as defined in any one of embodiments 47-75 to the infection, wherein application

of the composition washes or irrigates the wound.

90. A method to treat and/or provide relief from an infection in an individual, the method comprising,

1-46 or applying the components of a kit as defined in any one of embodiments 47-75 to the infection,

wherein application of the composition treats and/or provides relief from the infection.

91. A method to wash or irrigate an inflammation in an individual, the method comprising, consisting

applying the components of a kit as defined in any one of embodiments 47-75 to the inflammation,

wherein application of the composition washes or irrigates the inflammation.

92. A method to treat and/or provide relief from an inflammation in an individual, the method comprising,

1-46 or applying the components of a kit as defined in any one of embodiments 47-75 to the

inflammation, wherein application of the composition treats and/or provides relief from the

inflammation.

93. A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in washing or irrigating a wound in an individual.

94. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to wash or irrigate a wound in an individual.

95. A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in treating and/or providing relief from a wound in an

individual.

96. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to treat and/or provide relief from a wound in an individual.

97.A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in washing or irrigating an infection in an individual.

98. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to wash or irrigate an infection in an individual.

99. A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in treating and/or providing relief from an infection

in an individual.

100. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to treat and/or provide relief from an infection in an

individual.

WO wo 2020/252433 PCT/US2020/037665 PCT/US2020/037665 163

101. A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in washing or irrigating an inflammation in an

individual.

102. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to wash or irrigate an inflammation in an individual.

103. A composition as defined in any one of embodiments 1-46 or applying the components of a kit as

defined in any one of embodiments 47-75 for use in treating and/or providing relief from an

inflammation in an individual.

104. Use of a composition as defined in any one of embodiments 1-46 or applying the components of a kit

as defined in any one of embodiments 47-75 to treat and/or provide relief from an inflammation in an

individual.

105. The method of any one of embodiments 87-92, or the use of any one of embodiments 93-104, wherein

application of the composition is applied topically or administered enterally or parenterally.

106. The method of any one of embodiments 97-92, or the use of any one of embodiments 93-104, wherein

the composition is applied daily, every other day, every third of day, once a week, multiple times per

week, once a month, multiple times per month, once a year or multiple times per year, as desired.

107. The method of any one of embodiments 87-92, or the use of any one of embodiments 93-104, wherein

the composition is applied multiple times per day.

108. The method of any one of embodiments 87-92, or the use of any one of embodiments 93-104, wherein

the condition is a wound, an ophthalmic condition, a dermatological condition, an inflammation, an

ache or a pain.

EXAMPLES

[297] The following non-limiting examples are provided for illustrative purposes only in order to facilitate

a more complete understanding of representative embodiments now contemplated. These examples

should not be construed to limit any of the embodiments described in the present specification, including

those pertaining to the compositions, methods and uses disclosed herein.

Example 1

Compositions

[298] This example illustrates how to formulate a microbiostatic composition disclosed herein. The

components listed below were mixed at ambient temperature using a high shear mixer until the mixture

was uniform. The pH of the mixture was adjusted as shown. For hypochlorous acid amounts, unless

indicated otherwise, the amount is the final concentration after mixing with other components. A

microbiostatic composition disclosed herein is produced as follows: 1) for a formulation disclosed in Tables

1-15 comprising hypochlorous acid/FAC, the formulation is diluted, e.g. with water, so that the concentration

of hypochlorous acid/FAC is no more than 0.5 ppm, such as, e.g. between 0.01 ppm to 0.3 ppm; 2) for a

formulation disclosed in Tables 1-15 not containing hypochlorous acid/FAC, the formulation is diluted, e.g.

with water, 10-fold to 100-fold.

Table 1. Composition Formulations Component F1 F2 F3 F4 F5 F6 Hypochlorous Acid 84 ppm 84 ppm 84 ppm 97 ppm 97 ppm 97 ppm Polyhexamethylene biguanide 0.25% 0.5% 1.0% Isopropyl Alcohol - - - Glutaraldehyde - 0.1% - 0.25% - 0.5% - - - Water q.s. q.s. q.s. - q.s. - q.s. - q.s.

5.8 6.2 6.25 6.85 6.5 6.7 pH

Table 2. Composition Formulations Component F7 F8 F9 F10 F10 F11 F12 Hypochlorous Acid 97 ppm 97 ppm 97 ppm 97 ppm 97 ppm 97 ppm Polyhexamethylene biguanide Isopropyl Alcohol - - - - - - Glutaraldehyde - 0.5% - 0.5% - 0.5% - 0.25% - 0.25% - 0.25% Sodium Lauryl Sulfate 2% 2% 2% 2% 2% 2% 2% Water q.s. q.s. q.s. q.s. q.s. q.s.

3 4 5 3.5 4.5 5.5 pH

Table 3. Composition Formulations Component F13 F14 F15 F16 F17 F18 Hypochlorous Acid 66 ppm 76 ppm 80 ppm 82 ppm 73 ppm 75 ppm Polyhexamethylene biguanide Isopropyl Alcohol - - - - - - Glutaraldehyde - 0.5% - 0.5% - 0.5% - 0.25% - 0.25% - 0.25% Water q.s. q.s. q.s. q.s. q.s. q.s.

3 4 5 5 3.5 4.5 5.5 pH

Table 4. Composition Formulations Component F19 F20 F21 F22 F23 F24 Hypochlorous Acid 22 ppm 18 ppm 16 ppm 11 ppm Polyhexamethylene Biguanide 1.25% 1.5% 1.75% 2.0% - 2.0% - 3.0% Isopropyl Alcohol 70% 70% Glutaraldehyde - - - - Water - q.s. - q.s. - q.s. - q.s. q.s. - q.s. - q.s.

pH 5.75 6.6 6.4 6.9 ND ND ND, Not Determined.

Table 5. Composition Formulations Component F25 F26 F27 F28 F29 F30 Hypochlorous Acid Polyhexamethylene Biguanide - 4.0% - 6.0% - 8.0% - 10% 10% - 0.25% I 0.5% Isopropyl Alcohol 70% 70% 70% 70% 70% 70% Glutaraldehyde

Water - q.s. - q.s. q.s. - q.s. - q.s. - q.s.

pH 6.3 6.4 6.4 ND ND ND

WO wo 2020/252433 PCT/US2020/037665 165

ND, Not Determined.

Table 6. Composition Formulations Component F31 F32 F33 F34 F35 F36 Hypochlorous Acid Polyhexamethylene Biguanide - 0.75% - 1.0% - 1.25% - 1.5% - 1.75% - 2.0% Isopropyl Alcohol 70% 70% 70% 70% 70% 70% Glutaraldehyde

Water - q.s. - q.s. - q.s. - q.s. - q.s. - q.s.

pH ND ND ND ND ND ND ND, Not Determined.

Table 7. Composition Formulations Component F37 F38 F39 F40 F41 F42 Hypochlorous Acid 96 ppm 94 ppm 92 ppm 90 ppm 98 ppm 96 ppm Polyhexamethylene Biguanide 4.0% 6.0% 8.0% 10.0% 2.0% 4.0% Isopropyl Alcohol

Glutaraldehyde - - - - - - Water - q.s. - q.s. - q.s. - q.s. - q.s. - q.s.

pH ND ND ND ND ND ND ND ND, Not Determined.

Table 8. Composition Formulations Component F43 F44 F45 F46 F47 F48 Hypochlorous Acid 94 ppm 92 ppm 90 ppm 46 ppm 58 ppm Polyhexamethylene Biguanide 6.0% 8.0% 10.0% 0.25% 0.25% 0.25% Isopropyl Alcohol 40% Glutaraldehyde - - - - - Water - q.s. - q.s. q.s. q.s. q.s. - q.s.

6.8 6.5 5.4 pH ND ND ND ND, Not Determined.

Table 9. Composition Formulations Component F49 F50 F51 F52 F53 F54 Hypochlorous Acid 83 ppm 56 ppm Polyhexamethylene Biguanide - 0.25% 0.01% 0.001% 0.05% 0,01% Isopropyl Alcohol 20% 70% 70% 70% Glutaraldehyde - - Water - q.s. - q.s. - I q.s. - I q.s. - q.s. - q.s.

pH 5.5 5.8 5.8 6.0 6.6 7.4

Table 10. Composition Formulations Component F55 F56 F57 F58 F59 F60 Hypochlorous Acid Polyhexamethylene Biguanide 8.0% 9.5% 5.0% 10.0% 10.0% 10.0% Isopropyl Alcohol 70% 70% 70% 65% 60% 50% Glutaraldehyde 2.0% I wo 2020/252433 WO PCT/US2020/037665 166 ortho-Phthalaldehyde 0.5% Benzalkonium Chloride - - 5.0% - 5.0% - - Water - q.s. - q.s. q.s. q.s. - q.s. - q.s.

pH 4.0 3.9 7.7 7.3 6.0 6.0

Table 11. Composition Formulations Component F61 F62 F63 F64 F65 F66 Hypochlorous Acid

Polyhexamethylene Biguanide - 10.0% - 10.0% - 10.0% - 10.0% - 1% - 2% Isopropyl Alcohol 25.0% 33.33% 50% 50% Ethanol - - 50.0% 25.0% 16.67% Glutaraldehyde - - - Water - q.s. - q.s. - q.s. - q.s. - q.s. - q.s.

pH ND 6.15 6.38 6.29 6.01 6.19 ND, Not Determined.

Table 12. Composition Formulations Component F67 F68 F69 F70 F71 F97 Hypochlorous Acid 530 ppm* Polyhexamethylene Biguanide - 4.0% 6.0% - 8.0% 10.0% - 10.0% 500 ppm Isopropyl Alcohol 50.0% 50.0% 30.0% 40% Ethanol - 50.0% - Cetylpyridinium Chloride - - - - - Glutaraldehyde - - - - - - Water - q.s. - q.s. - q.s. - q.s. - q.s. - q.s.

pH 6.29 6.20 6.40 6.34 6.23 2.8 * HOCI concentration prior to mixing.

Table 13. Formulations for Composition

Component F98 F99 F111 F113 F114 F136 Hypochlorous Acid 107 ppm* 281 ppm* Polyhexamethylene Biguanide - 1% - 1% - 0.12% 1200 ppm 1200 ppm - 10% 10% Isopropyl Alcohol 50% 20% 15% 45% Ethanol - - Cetylpyridinium Chloride - - - - - - - 5.0% Glutaraldehyde - - - - - Water - q.s. - - q.s. - q.s. - q.s - q.s. - q.s.

pH 6.8 6.3 7.60 5.37 3.7 8.29 * HOCI concentration prior to mixing.

Table 14. Composition Formulations Component F137 F159 F160 F161 F162 F164 Hypochlorous Acid Polyhexamethylene Biguanide - 6.0% - 5.0% - 10% - 10% - - Isopropyl Alcohol 50.0% 5.0% 5.0% 5.0% - 5.0% - 50.0% Ethanol Cetylpyridinium Chloride - 4.0% I 5.0% - - I 10% - 10% - wo 2020/252433 WO PCT/US2020/037665 167 167

Glutaraldehyde

TRITON® CG-110 TRITON CG-110 - 2.0% - 2.0% - 2.0% Water q.s. q.s. q.s. q.s. q.s. q.s.

7.8 7.3 7.0 7.0 7.3 7.3 6.2 5.6 pH

Table 15. Composition Formulations Component F165 F166 F167 Hypochlorous Acid

Polyhexamethylene Biguanide - 10% Isopropyl Alcohol 50% Ethanol Cetylpyridinium Chloride 6.0% 10% Glutaraldehyde

TRITON® CG-110 TRITON CG-110 - 2% 2% 2% Poloxamer-407 2% 2% Water - q.s. q.s. q.s.

6.1 pH ND ND ND, Not Determined

Example 2

Compositions

[299] This example illustrates how to formulate a microbiostatic composition disclosed herein. The

was uniform. The pH of the mixture was adjusted as shown. A microbiostatic composition disclosed herein

is produced as follows: 1) for a formulation disclosed in Tables 16-57 comprising hypochlorous acid/FAC,

the formulation is diluted, e.g. with water, so that the concentration of hypochlorous acid/FAC is no more

than 0.5 ppm, such as, e.g. between 0.01 ppm to 0.3 ppm; 2) for a formulation disclosed in Tables 1-15 not

containing hypochlorous acid/FAC, the formulation is diluted, e.g. with water, 10-fold to 100-fold.

Table 16. Composition Formulations Component F72 F73 F74 F75 F76 F77 Hypochlorous Acid/FAC 273 ppm 273 ppm 273 ppm 273 ppm 273 ppm 273 ppm Titanium dioxide particles 50 ppm 100 ppm Zinc oxide particles - 50 ppm - 100 ppm - - - 100-ppm 200 ppm Water - q.s. - q.s. - q.s. - q.s. q.s. q.s.

5.5 6.3 4.1 3.9 3.9 3.6 3.6 pH a DTSACI: :Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 17. Composition Formulations Component F78 F79 F80 F81 F82 F83 Hypochlorous Acid/FAC 273 ppm 273 ppm 273 ppm Copper oxide particles 200 ppm 300 ppm - - - DTSACI Superscript(a)

- 300 ppm - 1% - 5% - 1% - - wo 2020/252433 WO PCT/US2020/037665 168

Isopropanol 50% 50% 50% 98% Methanol Methanol - - - 2% 6% 1% Water - q.s. - q.s. - q.s. q.s. q.s. q.s.

4.8 5.3 3.4 3.4 5.5 5.5 5.1 6.6 pH a DTSACI: Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 18. Composition Formulations Component F84 F85 F86 F87 F88 F89 Hypochlorous Acid/FAC 273 ppm 273 ppm 273 ppm 273 ppm 530 ppm Titanium dioxide particles 200 ppm - DTSACI - - - - 5% - Copper (I) chloride - - 300 ppm - - - Copper (II) chloride - - 300 ppm - - - Zinc chloride 300-ppm - - - - Silver nitrate - - - - - 300 ppm Isopropanol - - - - - 88.4% Methanol - - - I 6.6% - Water - q.s. - q.s - q.s - q.s. q.s. - q.s.

3.9 4.4 3.9 3.6 3.6 6.4 3.7 pH a DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammoniumchloride in 58% methanol.

Table 19. Composition Formulations

Component F90 F91 F92 F93 F94 F95A Hypochlorous Acid/FAC 530 ppm 530 ppm 530 ppm 530 ppm 530 ppm Iron oxide particles 300 ppm - Titanium dioxide particles 100-ppm - - - - DTSACI 500 ppm Didodecyldimethyl ammonium - - - 10% - 300 ppm - 300 ppm chloride

Silver nitrate 200 ppm - - Isopropanol - - 50% - - - Water - q.s. - q.s q.s - q.s. - q.s. - q.s.

3.5 3.8 8.4 3.7 3.5 3.4 pH a DTSACI: :Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol or water.

Table 20. Composition Formulations Component F96 F100 F101 F102 F103 F104 Hypochlorous Acid/FAC 107 ppm 530 ppm 530 ppm 530 ppm Silver oxide particles 200 ppm - Zinc oxide particles - 200 ppm - - - 100 ppm - - 5% Didodecyldimethyl ammonium - - - - chloride 5% Copper (II) chloride - - 500 ppm - - - Potassium chloride - - 4000 ppm - - - Polyhexamethylene biguanide - - - - 10% - 10% 10% Isopropanol - - - - 45% 45% Water - q.s. - q.s. - q.s. - q.s. q.s q.s wo 2020/252433 WO PCT/US2020/037665 169

3.7 3.4 3.7 6.4 8.3 6.8 pH a DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 21. Composition Formulations Component F105 F106 F107 F108 F109 F110 Hypochlorous Acid/FAC 530 ppm 530 ppm 530 ppm 530 ppm DTSACI Superscript(a)

4% - 1000 ppm Didodecyldimethyl ammonium - chloride 4% Potassium chloride 600 ppm 600 ppm 600 ppm Potassium phosphate - - 6000 ppm 2000 ppm 2000 ppm Polyhexamethylene biguanide - 6% - 6% Isopropanol 50% 50% - Water q.s. q.s. q.s. q.s. - q.s q.s

pH 6.6 8.5 11.9 7.7 8.9 4.4 pH a DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 22. Composition Formulations Component F112 F115 F116 F117 F118 F119 Hypochlorous Acid/FAC 281 ppm 530 ppm 1933 ppm Chlorhexidine 0.06% 0.12%

DTSACI - - - - 2000 ppm Zinc acetate - - - 0.14% 300-ppm 600 ppm Polyhexamethylene biguanide - 0.06% - 0.12% - Ethanol 15% - 15% 15% - - Water q.s q.s q.s - q.s q.s q.s

7.6 7.3 7.3 6.9 5.4 5.4 3.7 pH a DTSACI: Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 23. Composition Formulations Component F120 F121 F122 F123 F124 F125 Hypochlorous Acid/FAC 105 ppm 105 ppm 500 ppm 281 ppm 281 ppm 530 ppm Titanium dioxide particles

Copper zinc iron oxide particles - 300 ppm - - DTSACI - 300 ppm 500 ppm Potassium chloride 600 ppm 120-ppm Potassium phosphate 2000 ppm 400 ppm Silver nitrate 300 ppm 500 ppm Zinc Sulfate - - 600 ppm Water - q.s. - q.s q.s. q.s - q.s - q.s

7.0 7.6 3.7 5.2 5.2 3.5 3.5 pH a DTSACI: Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 24. Composition Formulations Component F126 F127 F128 F129 F130 F131 Hypochlorous Acid/FAC 281 ppm 530 ppm 600 ppm 278 ppm 1,933 ppm 600 ppm Titanium dioxide particles 100 ppm 100 ppm - - wo 2020/252433 WO PCT/US2020/037665 170

DTSACI 300 ppm 500 ppm Silver nitrate 200 ppm 300 ppm - Poly(acrylic acid) - 45% - 2% - 2% - 2% Water - q.s. - q.s q.s. q.s. q.s. q.s.

3.7 3.5 3.5 2.9 2.9 2.8 2.8 2.9 2.9 7.3 7.3 pH a DTSACI: Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 25. Composition Formulations Component F132 F133 F134 F135 F138 F139 Hypochlorous Acid/FAC 600 ppm 600 ppm 1,933 ppm 600 ppm 600 ppm 600 ppm 600 ppm 600 ppm 1,200 ppm Silver nitrate 600 ppm Zinc sulfate - 600 ppm - - - Polyhexamethylene biguanide - - - 600-ppm - ppm 1,200 Poly(acrylic acid) 2% 2% - 2% Cetylpyrdinium chloride 600 ppm - Water q.s. - q.s q.s. q.s. q.s. - q.s

2.7 2.9 2.9 2.9 4.0 3.0 3.0 3.0 pH a DTSACI: I:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 26. Composition Formulations Component F140 F141 F142 F143 F144 F145 Hypochlorous Acid/FAC 600 ppm 600 ppm 600 ppm 500 ppm 500 ppm 500 ppm Copper zinc iron oxide particles 600 ppm 300 ppm 600 ppm - 1,200 ppm 600 ppm - TTSACI - 500-ppm Polyhexamethylene biguanide - - - - ortho-phthalaldehyde 600 ppm 600 ppm 1,200 ppm Water q.s. q.s. q.s. q.s. q.s. q.s

2.6 3.4 3.2 5.2 5.2 5.0 3.4 pH a DTSACI: Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol. b TTSACI: Tetradecyldimethyl-(3-trimethoxysilyl)propyl ammonium chloride in water.

Table 27. Composition Formulations Component F146 F147 F148 F149 F150 F151A Hypochlorous Acid/FAC 2,200 ppm 500 ppm 500 ppm 500 ppm 500 ppm 500 ppm Copper zinc iron oxide particles 300 ppm 250 ppm - 250 ppm - 125 ppm - 50 ppm - 25 ppm Water - q.s. q.s. q.s. q.s. q.s. - q.s

4.6 3.6 3.7 3.7 3.6 4.7 pH a DTSACI: Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 28. Composition Formulations Component F152 F153 F154 F155 F156 F157 Hypochlorous Acid/FAC 500 ppm 500 ppm 500 ppm 500 ppm 500 ppm Copper zinc iron oxide particles 200 ppm 150 ppm 100 ppm 50 ppm 25 ppm - DTSACI Superscript(a)

- 10% - - - - wo 2020/252433 WO PCT/US2020/037665 171

Isopropanol 50% Water - q.s. - q.s. - q.s. - q.s. - q.s. q.s

4.3 3.8 3.2 3.8 3.7 4.6 pH a DTSACI: :Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 29. Composition Formulations Component F158 F163 F168 F169 F170 F171 DTSACI 20% 10% Didodecyldimethylammonium - - - - chloride 5% Polyhexamethylene biguanide 5% Isopropanol 5% - - 50% - - - 5% Methanol - 7% - 50% - TRITON CG-110 - 3% - - - Poloxamer-407 - - 2% Quaternium-18 - - 6% 10% - 10% Water - q.s. q.s. q.s. q.s. q.s. q.s.

pH 6.4 ND ND ND ND ND a DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 30. Composition Formulations Component F172 F173 F177 F178 F179 F180 Hypochlorous Acid/FAC 540 ppm DTSACI Superscript(a)

500 ppm 20% - - Di-n-alkyldimethyl ammonium - 500 ppm - - - chloride

Didodecyldimethyl ammonium - - - I - chloride 500 ppm

Methanol - - - - 7% Copper (II) chloride - - 200-ppm Iron (II) chloride or Iron (III)

chloride 200 ppm

Silver nitrate - - - - - 500 ppm Zinc acetate 500 ppm - Zinc chloride - - - - 200-ppm Water - q.s. - q.s. - q.s. q.s. - q.s. q.s.

pH 5.4 6.4 7.6 6.2 6.9 2.6 a DTSACI: :Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 31. Composition Formulations Component F181 F182 F183 F184 F185 F186 Hypochlorous Acid/FAC 540 ppm 540 ppm 540 ppm 540 ppm 540 ppm 540 ppm DTSACI 500 ppm 500 ppm 500 ppm 500 ppm 500 ppm Copper (III) chloride - 200 ppm Iron (II) chloride or Iron (III) 200 ppm - I - - - chloride

Zinc chloride 200 ppm Water q.s. - q.s. - q.s. - q.s. - q.s. - q.s.

wo 2020/252433 WO PCT/US2020/037665 172

2.4 5.9 5.4 6.0 5.1 6.4 pH a DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 32. Composition Formulations Component F187 F188 F189 F190 F191 F192 Hypochlorous Acid/FAC 540 ppm 350 ppm 350 ppm 260 ppm 260 ppm 190 ppm DTSACI 300 ppm 500 ppm 300 ppm 500 ppm 300 ppm 500 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.5 5.7 5.5 5.2 5.2 5.4 5.4 pH a DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammoniume chloride in 58% methanol.

Table 33. Composition Formulations Component F193 F194 F195 F95B F95C F95D Hypochlorous Acid/FAC 190 ppm 100 ppm 100 ppm 530 ppm 75 ppm 75 ppm DTSACI 300 ppm 500 ppm 300 ppm 500 ppm 300 ppm DTSACI 530 ppm Water q.s. - q.s. - q.s. q.s. - q.s. q.s.

pH 5.5 5.8 5.4 3.2 5.8 5.4

a DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol. b DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammoniumo chloride in water.

Table 34. Composition Formulations Component F95E F95F F95F F95G F95H F951 F95I F95J Hypochlorous Acid/FAC 50 ppm 50 ppm 40 ppm 40 ppm 35 ppm 35 ppm DTSACI Superscript(a)

500 ppm 300 ppm 500 ppm 300 ppm 500 ppm 300 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.8 5.4 5.8 5.4 5.8 5.4 pH a DTSACI: Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 35. Composition Formulations Component F95K F95L F95L F95M F95N F950 F95P Hypochlorous Acid/FAC 25 ppm 25 ppm 10 ppm 10 ppm 5 ppm 5 ppm DTSACI Superscript(a)

5.8 5.4 5.8 5.4 5.8 5.4 pH a DTSACI:Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride in 58% methanol.

Table 36. Composition Formulations Component F151B F151C F151D F151E F151E F151F F151G Hypochlorous Acid/FAC 350 ppm 260 ppm 190 ppm 100 ppm 75 ppm 50 ppm Copper zinc iron oxide particles 250 ppm 250 ppm 250 ppm 250 ppm 250 ppm 250 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

4.7 4.7 4.7 4.7 4.7 4.7 pH

Table 37. Composition Formulations

Component F151H F1511 F151I F151J F151K F151L F180A Hypochlorous Acid/FAC 40 ppm 35 ppm 25 ppm 10 ppm 5 ppm 350 ppm

WO wo 2020/252433 PCT/US2020/037665 173 173

Copper zinc iron oxide particles 250 ppm 250 ppm 250 ppm 250 ppm 250 ppm Copper (II) chloride 200 ppm Iron (II) chloride or Iron (III)

chloride 200 ppm

Zinc chloride - 200 ppm Water q.s. - q.s. - q.s. q.s. - q.s. q.s.

pH 4.7 4.7 4.7 4.7 4.7 2.6

Table 38. Composition Formulations Component F180B F180C F180D F180E F180E F180F F180G Hypochlorous Acid/FAC 260 ppm 190 ppm 100 ppm 75 ppm 50 ppm 40 ppm Copper (II) chloride 200 ppm 200 ppm 200 ppm 200 ppm Iron (II) chloride or Iron (III)

chloride - 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm

Zinc chloride 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm 200 ppm Water 200 ppm 200 ppm q.s. q.s. q.s. q.s.

2.6 2.6 2.6 2.6 pH q.s. q.s.

2.6 2.6

Table 39. Composition Formulations Component F180H F1801 F180I F180J F180K Hypochlorous Acid/FAC 35 ppm 25 ppm 10 ppm 5 ppm Copper (II) chloride 200 ppm 200 ppm 200 ppm 200 ppm Iron (II) chloride or Iron (III)

chloride 200 ppm 200 ppm 200 ppm 200 ppm

Zinc chloride 200 ppm 200 ppm 200 ppm 200 ppm Water q.s. q.s. q.s. q.s.

2.6 2.6 2.6 2.6 pH

Table 40. Composition Formulations Component F461A F461B F461C F461D F461E F461E F461F Hypochlorous Acid/FAC 310 ppm 290 ppm 260 ppm 190 ppm 100 ppm 75 ppm Copper (II) chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Zinc chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Calcium Oxide 160 ppm 120 ppm 80 ppm 60 ppm 40 ppm 20 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

7.1 pH ND ND ND ND ND

Table 41. Composition Formulations Component F461G F461H F4611 F461J F461K F461L Hypochlorous Acid/FAC 50 ppm 40 ppm 35 ppm 25 ppm 10 ppm 5 ppm Copper (II) chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Zinc chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Calcium Oxide 20 ppm 20 ppm 10 ppm 10 ppm 5 ppm 5 ppm Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND

WO wo 2020/252433 PCT/US2020/037665 174 174

Table 42. Composition Formulations Component F469A F469B F469C F469D F469E F469F Hypochlorous Acid/FAC 310 ppm 290 ppm 260 ppm 190 ppm 100 ppm 75 ppm Copper (II) chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Zinc chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Calcium Oxide 160 ppm 120 ppm 80 ppm 60 ppm 40 ppm 20 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.0 pH ND ND ND ND ND ND

Table 43. Composition Formulations Component F469G F469H F4691 F469J F469K F469L Hypochlorous Acid/FAC 50 ppm 40 ppm 35 ppm 25 ppm 10 ppm 5 ppm Copper (II) chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Zinc chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Calcium Oxide 20 ppm 20 ppm 10 ppm 10 ppm 5 ppm 5 ppm Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND

Table 44. Composition Formulations Component F471A F471B F471C F471D F471E F471F Hypochlorous Acid/FAC 310 ppm 290 ppm 260 ppm 190 ppm 100 ppm 75 ppm Copper (II) chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Zinc chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Calcium Oxide 160 ppm 120 ppm 80 ppm 60 ppm 40 ppm 20 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

pH 5.2 ND ND ND ND ND ND

Table 45. Composition Formulations Component F471G F471H F471I F471 F471J F471K F471L F471L Hypochlorous Acid/FAC 50 ppm 40 ppm 35 ppm 25 ppm 10 ppm 5 ppm Copper (II) chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Zinc chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Calcium Oxide 20 ppm 20 ppm 10 ppm 10 ppm 5 ppm 5 ppm Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND

Table 46. Composition Formulations Component F486A F486B F486C F486D F486E F486F Hypochlorous Acid/FAC 310 ppm 290 ppm 260 ppm 190 ppm 100 ppm 75 ppm Copper (II) chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Zinc chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Calcium Phosphate 80 ppm 60 ppm 40 ppm 20 ppm 10 ppm 10 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.0 pH ND ND ND ND ND

Table 47. Composition Formulations Component F486G F486H F4861 F486I F486J F486K F486L

WO wo 2020/252433 PCT/US2020/037665 175 175

Hypochlorous Acid/FAC 50 ppm 40 ppm 35 ppm 25 ppm 10 ppm 5 ppm Copper (II) chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Zinc chloride 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm 120 ppm Calcium Phosphate 10 ppm 10 ppm 5 ppm 5 ppm 5 ppm 5 ppm Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND

Table 48. Composition Formulations Component F487A F487B F487B F487C F487D F487E F487F Hypochlorous Acid/FAC 90 ppm 80 ppm 70 ppm 60 ppm 50 ppm 40 ppm Copper (II) chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Zinc chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Calcium Oxide 160 ppm 120 ppm 80 ppm 60 ppm 40 ppm 20 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.0 pH ND ND ND ND ND ND

Table 49. Composition Formulations Component F487G F487H F4871 F487I F487J F487K F487L F487L Hypochlorous Acid/FAC 30 ppm 25 ppm 20 ppm 15 ppm 10 ppm 5 ppm Copper (II) chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Zinc chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Calcium Oxide 20 ppm 20 ppm 10 ppm 10 ppm 5 ppm 5 ppm Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND ND

Table 50. Composition Formulations Component F489A F489B F489C F489D F489E F489F Hypochlorous Acid/FAC 90 ppm 80 ppm 70 ppm 60 ppm 50 ppm 40 ppm Copper (II) chloride 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm Zinc chloride 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm Calcium Oxide 160 ppm 120 ppm 80 ppm 60 ppm 40 ppm 20 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.0 pH ND ND ND ND ND

Table 51. Composition Formulations Component F489G F489H F4891 F489I F489J F489K F489L F489L Hypochlorous Acid/FAC 30 ppm 25 ppm 20 ppm 15 ppm 10 ppm 5 ppm Copper (II) chloride 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm Zinc chloride 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm Calcium Oxide 20 ppm 20 ppm 10 ppm 10 ppm 5 ppm 5 ppm Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND

Table 52. Composition Formulations Component F491A F491B F491C F491D F491E F491F Hypochlorous Acid/FAC 90 ppm 80 ppm 70 ppm 60 ppm 50 ppm 40 ppm Copper (II) chloride 20 ppm 20 ppm 20 ppm 20 ppm 20 ppm 20 ppm

WO wo 2020/252433 PCT/US2020/037665 176

Zinc chloride 20 ppm 20 ppm 20 ppm 20 ppm 20 ppm 20 ppm Calcium Oxide 160 ppm 120 ppm 80 ppm 60 ppm 40 ppm 20 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.0 pH ND ND ND ND ND

Table 53. Composition Formulations Component F491G F491H F491H F491I F491 F491J F491K F491L F491L Hypochlorous Acid/FAC 30 ppm 25 ppm 20 ppm 15 ppm 10 ppm 5 ppm Copper (II) chloride 20 ppm 20 ppm 20 ppm 20 ppm 20 ppm 20 ppm Zinc chloride 20 ppm 20 ppm 20 ppm 20 ppm 20 ppm 20 ppm Calcium Oxide 20 ppm 20 ppm 10 ppm 10 ppm 5 ppm 5 ppm Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND

Table 54. Composition Formulations Component F493A F493B F493C F493D F493E F493F Hypochlorous Acid/FAC 310 ppm 290 ppm 260 ppm 190 ppm 100 ppm 75 ppm Copper (II) chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Zinc chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Calcium Phosphate 80 ppm 60 ppm 40 ppm 20 ppm 10 ppm 10 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.0 pH ND ND ND ND ND ND ND

Table 55. Composition Formulations Component F493G F493H F493I F493 F493J F493K F493L F493L Hypochlorous Acid/FAC 50 ppm 40 ppm 35 ppm 25 ppm 10 ppm 5 ppm Copper (II) chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Zinc chloride 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm 80 ppm Calcium Phosphate 10 ppm 10 ppm 5 ppm 5 ppm 5 ppm 5 ppm Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND

Table 56. Composition Formulations Component F494A F494B F494C F494D F494E F494F Hypochlorous Acid/FAC 310 ppm 290 ppm 260 ppm 190 ppm 100 ppm 75 ppm Copper (II) chloride 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm Zinc chloride 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm Calcium Phosphate 80 ppm 60 ppm 40 ppm 20 ppm 10 ppm 10 ppm Water q.s. q.s. q.s. q.s. q.s. q.s.

5.0 pH ND ND ND ND ND

Table 57. Composition Formulations Component F494G F494H F4941 F494I F494J F494K F494L F494L Hypochlorous Acid/FAC 50 ppm 40 ppm 35 ppm 25 ppm 10 ppm 5 ppm Copper (II) chloride 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm Zinc chloride 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm 40 ppm Calcium Phosphate 10 ppm 10 ppm 5 ppm 5 ppm 5 ppm 5 ppm

Water q.s. q.s. q.s. q.s. q.s q.s

pH ND ND ND ND ND ND

Example 3 Microbiostatic Assay

[300] This example illustrates how to conduct a microbiostatic assay using a composition disclosed

herein.

[301] To prepare stock cultures of pathogenic bacteria, 10 mL nutrient broth was inoculated using a stock

culture of Staphylococcus aureus (ATCC 29213) and incubated on an orbital shaker for 24+2 hours at

approximately 150 rpm at 36+1°C. This culture was used to inoculate Tryptic Soy Agar (TSA) plates. Each

plate was inoculated with the culture. Each plate was incubated for up to 48 hours at 30°C to 35°C.

Following incubation, suspensions were prepared by adding growth from TSA plates into a tube containing

sterile water. Each tube was then vortexed and an aliquot was serially diluted and plated onto TSA plate

for further numeration. The final numeration count uses aliquot volume, count obtained on TSA plate and

the total original volume of culture. The remaining amount in the tube was used to inoculate actual test

samples.

[302] To assay for microbiostatic activity, an antimicrobial effectiveness test according to the U.S.

Pharmacopeia Chapter 51 (USP-51) protocols were performed. Each composition was tested over a seven

log dilution series of pathogen bacteria assays by preparing samples containing 200 pL of a pathogenic

bacteria suspension, 20 pL of an organic load, and 20,000 pL of a composition disclosed herein. Control

samples contain 200 pL of a pathogenic bacteria suspension, 20 pL of an organic load prepared according

to USP 51, and 20,000 pL of water. Composition 1 comprised 0.25 ppm hypochlorous acid while Composition 2 comprised 0.5 ppm hypochlorous acid. Samples were continuously incubated for 28 days,

with assay aliquots removed on Day 0, 1, 7, 14, and 28. Once removed, each assay aliquot was

immediately mixed with a neutralizing agent to inhibit the activity of the microbial agent being tested. The

treated assay samples were serially diluted 1 in 10 over a 6-fold range and plated on petri dishes containing

TSA growth medium and incubated at 30°C to 35°C for up to 48 hours. The presence of viable bacteria

was then measured by counting the number of colonies observed on the agar medium for each assay

sample. Log reduction is determined by taking the log10 of the number calculated by taking the number of

viable pathogenic bacteria colonies present in the assay sample without serum divided by the number of

viable pathogenic bacteria colonies present in the assay sample with serum. In this assay, a composition

indicative in having microbiostatic activity is one exhibiting a minimum of a 2-log reduction in the amount

of pathogenic bacteria present in an assay sample at Day 14 when compared to Day 0. A greater than

two-fold log reduction in the number of pathogenic bacteria present in an assay sample was indicative of

microbiocidal activity (i.e., the composition was not microbiostatic). The results of this series of experiments

are shown in Table 58.

[303] Initial assays demonstrated that compositions comprising at least 0.5 ppm of hypochlorous acid or

free available chlorine exhibited on average a 1 X 107 log reduction, or 7-log reduction, in pathogen bacteria.

These results indicate that the minimum microbiocidal concentration of hypochlorous acid or free available

chlorine in the disclosed compositions is approximately 0.5 ppm. However, compositions comprising

hypochlorous acid or free available chlorine below 0.5 ppm were microbiostatic. For example, as shown in

Table 58, Composition 1, which contained 0.25 ppm hypochlorous acid, demonstrated a microbiostatic

activity.

Table 58. Microbiostatic Assay

Control Composition 1 Composition 2 Day Log Log Log Log Log Log Recovery Reduction Recovery Reduction Recovery Reduction 0 7.26 0 7.34 0 3.91 3.35 1 7.08 7.15 7 0 0 0 7 7 0 7 0 0 7

14 7.04 0 4.08 2.96 0 7

[304] In closing, it is to be understood that although aspects of the present specification are highlighted

by referring to specific embodiments, one skilled in the art will readily appreciate that these disclosed

embodiments are only illustrative of the principles of the subject matter disclosed herein. Therefore, it

should be understood that the disclosed subject matter is in no way limited to a particular compound,

composition, article, apparatus, methodology, protocol, and/or reagent, etc., described herein, unless

expressly stated as such. In addition, those of ordinary skill in the art will recognize that certain changes,

modifications, permutations, alterations, additions, subtractions and sub-combinations thereof can be made

in accordance with the teachings herein without departing from the spirit of the present specification. It is

therefore intended that the following appended claims and claims hereafter introduced are interpreted to

include all such changes, modifications, permutations, alterations, additions, subtractions and sub-

combinations as are within their true spirit and scope.

[305] Certain embodiments of the present invention are described herein, including the best mode known

to the inventors for carrying out the invention. Of course, variations on these described embodiments will

become apparent to those of ordinary skill in the art upon reading the foregoing description. The inventor

expects skilled artisans to employ such variations as appropriate, and the inventors intend for the present

invention to be practiced otherwise than specifically described herein. Accordingly, this invention includes

all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted

by applicable law. Moreover, any combination of the above-described embodiments in all possible

variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly

contradicted by context.

[306] Groupings of alternative embodiments, elements, or steps of the present invention are not to be

construed as limitations. Each group member may be referred to and claimed individually or in any

combination with other group members disclosed herein. It is anticipated that one or more members of a

group may be included in, or deleted from, a group for reasons of convenience and/or patentability. When

any such inclusion or deletion occurs, the specification is deemed to contain the group as modified thus

fulfilling the written description of all Markush groups used in the appended claims.

[307] Unless otherwise indicated, all numbers expressing a characteristic, item, quantity, parameter,

property, term, and so forth used in the present specification and claims are to be understood as being

modified in all instances by the term "about." As used herein, the term "about" means that the characteristic,

item, quantity, parameter, property, or term so qualified encompasses a range of plus or minus ten percent

above and below the value of the stated characteristic, item, quantity, parameter, property, or term.

Accordingly, unless indicated to the contrary, the numerical parameters set forth in the specification and

attached claims are approximations that may vary. For instance, as mass spectrometry instruments can

vary slightly in determining the mass of a given analyte, the term "about" in the context of the mass of an

ion or the mass/charge ratio of an ion refers to +/-0.50 atomic mass unit. At the very least, and not as an

attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical

indication should at least be construed in light of the number of reported significant digits and by applying

ordinary rounding techniques.

[308] Use of the terms "may" or "can" in reference to an embodiment or aspect of an embodiment also

carries with it the alternative meaning of "may not" or "cannot." As such, if the present specification

discloses that an embodiment or an aspect of an embodiment may be or can be included as part of the

inventive subject matter, then the negative limitation or exclusionary proviso is also explicitly meant,

meaning that an embodiment or an aspect of an embodiment may not be or cannot be included as part of

the inventive subject matter. In a similar manner, use of the term "optionally" in reference to an embodiment

or aspect of an embodiment means that such embodiment or aspect of the embodiment may be included

as part of the inventive subject matter or may not be included as part of the inventive subject matter.

Whether such a negative limitation or exclusionary proviso applies will be based on whether the negative

limitation or exclusionary proviso is recited in the claimed subject matter.

[309] Notwithstanding that the numerical ranges and values setting forth the broad scope of the invention

are approximations, the numerical ranges and values set forth in the specific examples are reported as

precisely as possible. Any numerical range or value, however, inherently contains certain errors

necessarily resulting from the standard deviation found in their respective testing measurements. Recitation

of numerical ranges of values herein is merely intended to serve as a shorthand method of referring

individually to each separate numerical value falling within the range. Unless otherwise indicated herein,

each individual value of a numerical range is incorporated into the present specification as if it were

individually recited herein.

[310] The terms "a," "an," "the" and similar references used in the context of describing the present

invention (especially in the context of the following claims) are to be construed to cover both the singular

and the plural, unless otherwise indicated herein or clearly contradicted by context. Further, ordinal

indicators - such as "first," "second," "third," etc. - for identified elements are used to distinguish between

the elements, and do not indicate or imply a required or limited number of such elements, and do not

indicate a particular position or order of such elements unless otherwise specifically stated. All methods

described herein can be performed in any suitable order unless otherwise indicated herein or otherwise

WO wo 2020/252433 PCT/US2020/037665 PCT/US2020/037665 180

clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., "such as")

provided herein is intended merely to better illuminate the present invention and does not pose a limitation

on the scope of the invention otherwise claimed. No language in the present specification should be

construed as indicating any non-claimed element essential to the practice of the invention.

[311] When used in the claims, whether as filed or added per amendment, the open-ended transitional

term "comprising" (and equivalent open-ended transitional phrases thereof like including, containing and

having) encompasses all the expressly recited elements, limitations, steps and/or features alone or in

combination with unrecited subject matter; the named elements, limitations and/or features are essential,

but other unnamed elements, limitations and/or features may be added and still form a construct within the

scope of the claim. Specific embodiments disclosed herein may be further limited in the claims using the

closed-ended transitional phrases "consisting of" or "consisting essentially of" in lieu of or as an amended

for "comprising." When used in the claims, whether as filed or added per amendment, the closed-ended

transitional phrase "consisting of" excludes any element, limitation, step, or feature not expressly recited in

the claims. The closed-ended transitional phrase "consisting essentially of" limits the scope of a claim to

the expressly recited elements, limitations, steps and/or features and any other elements, limitations, steps

and/or features that do not materially affect the basic and novel characteristic(s) of the claimed subject

matter. Thus, the meaning of the open-ended transitional phrase "comprising" is being defined as

encompassing all the specifically recited elements, limitations, steps and/or features as well as any optional,

additional unspecified ones. The meaning of the closed-ended transitional phrase "consisting of" is being

defined as only including those elements, limitations, steps and/or features specifically recited in the claim

whereas the meaning of the closed-ended transitional phrase "consisting essentially of" is being defined as

only including those elements, limitations, steps and/or features specifically recited in the claim and those

elements, limitations, steps and/or features that do not materially affect the basic and novel characteristic(s)

of the claimed subject matter. Therefore, the open-ended transitional phrase "comprising" (and equivalent

open-ended transitional phrases thereof) includes within its meaning, as a limiting case, claimed subject

matter specified by the closed-ended transitional phrases "consisting of" or "consisting essentially of." As

such embodiments described herein or so claimed with the phrase "comprising" are expressly or inherently

unambiguously described, enabled and supported herein for the phrases "consisting essentially of" and

"consisting of."

[312] All patents, patent publications, and other publications referenced and identified in the present

specification are individually and expressly incorporated herein by reference in their entirety for the purpose

of describing and disclosing, for example, the compositions and methodologies described in such

publications that might be used in connection with the present invention. These publications are provided

solely for their disclosure prior to the filing date of the present application. Nothing in this regard should be

construed as an admission that the inventors are not entitled to antedate such disclosure by virtue of prior

invention or for any other reason. All statements as to the date or representation as to the contents of these

documents is based on the information available to the applicants and does not constitute any admission

as to the correctness of the dates or contents of these documents.

[313] Lastly, the terminology used herein is for the purpose of describing particular embodiments only,

and is not intended to limit the scope of the present invention, which is defined solely by the claims.

Accordingly, the present invention is not limited to that precisely as shown and described.

Claims

CLAIMS 28 Jan 2026

1. A composition comprising:

a microbiostatic effective amount of a microbiostatic agent, the microbiostatic agent comprising at least about 0.1 ppm to less than 0.5 ppm of hypochlorous acid;

wherein the microbiostatic effect amount of the microbiostatic agent is a sub-lethal amount that restrains, prohibits or prevents microbial population growth for at least the first seven days of continuous exposure to the microbiostatic agent; and 2020291943

wherein the composition does not comprise a metal salt;

wherein the composition is a microbiostatic composition; and

wherein the pH of the composition is within a range of about pH 4 to about pH 6.5.

2. The composition according to Claim 1, wherein the hypochlorous acid is in an amount that does not eradicate the microbial population to a degree that achieves disinfection or sterilization.

3. The composition according to Claim 1 or 2, wherein the pH of the composition is within a range of about pH 4 to about pH 6.

4. The composition according to any one of Claims 1-3, wherein the pH of the composition is within a range of about pH 5 to about pH 6.

5. The composition according to any one of Claims 1-4, wherein the composition is made by a process comprising:

applying an electrical current to a cell comprising dissolved chlorine in water, and hydrolyzing of chlorine gas to produce the microbiostatic composition; wherein the cell comprises an anode chamber, a cathode chamber and a semi-permeable membrane separating the anode chamber from the cathode chamber.

6. The composition according to Claim 1-5, wherein the hypochlorous acid is in an amount of at least 0.1 ppm to at most 0.4 ppm.

7. The composition of any one of Claims 1-6, wherein the hypochlorous acid is in an amount of at least 0.1 ppm to at most 0.3 ppm.

8. The composition according to any one of Claims 1-4, wherein the hypochlorous acid is in an amount of about 0.25 ppm.

9. A method of reducing or inhibiting microbial growth in a device, the method comprising applying a composition as defined in any one of Claims 1-8 to the device, wherein application of the composition reduces or inhibits microbial population growth during use or storage of the device.

10. The method according to Claim 9, wherein the method restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbial population to a degree that achieves disinfection or sterilization.

11. The method according to Claim 9 or 10, wherein the device is a medical device, a 28 Jan 2026

residential device, a commercial device or an industrial device.

12. A method to wash or irrigate a surface area, the method comprising applying a composition as defined in any one of Claims 1-8 to the surface area, wherein application of the composition washes or irrigates the surface area and reduces or inhibits microbial population growth on the surface area.

13. The method according to Claim 12, wherein the surface area is a hard surface area or a biological surface area. 2020291943

14. The method according to Claim 12 or 13, wherein the method restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbial population to a degree that achieves disinfection or sterilization.

15. A method to treat or provide relief from an itch, an irritation, an inflammation, a wound, an ache, and/or a pain, the method comprising applying a composition as defined in any one of Claims 1-8 to a biological surface area, wherein application of the composition washes or irrigates the surface area and reduces or inhibits microbial population growth on the surface area.

16. The method according to any one of Claims 13-15, wherein the biological surface area is a tissue, a skin or a body cavity.

17. The method according to any one of Claims 13-15, wherein the biological surface area has a wound, an infection or an inflammation.

18. The method according to any one of Claims 13-17, wherein the composition is in an amount that restrains, prohibits, or otherwise prevents microbial population growth without eradicating the microbial population to a degree that achieves disinfection or sterilization.

19. A composition as defined in any one of Claims 1-8 for use in reducing or inhibiting microbial growth in a device.

20. The composition according to Claim 19, wherein the device is a medical device, a residential device, a commercial device or an industrial device.

21. A composition as defined in any one of Claims 1-8 for use in washing or irrigating a surface area.

22. The composition according to Claim 21, wherein the surface area is a hard surface area or a biological surface area.

23. The composition according to Claim 22, wherein the surface is a biological surface area, and wherein the biological surface area is a tissue, a skin, or a body cavity.

24. A composition as defined in any one of Claims 1-8 for use in treating or providing relief from an itch, an irritation, an inflammation, a wound, an ache, and/or a pain.

25. Use of a composition as defined in any one of Claims 1-8 in the manufacture of a medicament for washing or irrigating a biological surface area.

26. The use according to Claim 25, wherein the biological surface area is a tissue, a skin, or a body cavity.

27. Use of a composition as defined in any one of Claims 1-8 in the manufacture of a 28 Jan 2026

medicament for treating or providing relief from an itch, an irritation, an inflammation, a wound, an ache, and/or a pain.