AU539531B2 - Immunochemical assay for prostatic acid phosphatase - Google Patents
Immunochemical assay for prostatic acid phosphataseInfo
- Publication number
- AU539531B2 AU539531B2 AU85051/82A AU8505182A AU539531B2 AU 539531 B2 AU539531 B2 AU 539531B2 AU 85051/82 A AU85051/82 A AU 85051/82A AU 8505182 A AU8505182 A AU 8505182A AU 539531 B2 AU539531 B2 AU 539531B2
- Authority
- AU
- Australia
- Prior art keywords
- antibody
- isoenzyme
- precipitate
- sample
- acid phosphatase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/548—Carbohydrates, e.g. dextran
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Steroid Compounds (AREA)
- Amplifiers (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
1. Claims for the Contracting States BE, CH, DE, FR, GB, IT, LI, NL, SE A process for determining the amount of prostatic acid phosphatase isoenzyme in a biological fluid sample, characterized by a) incubating the sample with (i) a soluble first antibody which selectively binds immunologically to the isoenzyme in the sample without inhibiting the enzymatic activity of this isoenzyme, and (ii) an insolubilized antibody formed by coupling this second antibody to amylose whereby the second antibody selectively binds immunologically to the first antibody without inhibiting the enzymatic activity of the isoenzyme, to obtain a precipitate of a mixture of immunocomplexes which contain isoenzyme-first antibody-second antibody and first antibody-second antibody, and b) isolating the precipitate containing the immunocomplexes and c) measuring the isoenzyme activity in the precipitate. 1. Claims for the Contracting State AT A process for determining the amount of prostatic acid phosphatase isoenzyme in a biological fluid sample, characterized by a) incubating the sample with (i) a soluble first antibody which selectively binds immunologically to the isoenzyme in the sample without inhibiting the enzymatic activity of this isoenzyme, and (ii) an insolubilized antibody formed by coupling this second antibody to amylose, whereby the second antibody selectively binds immunologically to the first antibody without inhibiting the enzymatic activity of the isoenzyme, to obtain a precipitate of a mixture of immunocomplexes which contain isoenzyme-first antibody-second antibody and first antibody-second antibody, and b) isolating the precipitate containing the immunocomplexes and c) measuring the isoenzyme activity in the precipitate.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27622381A | 1981-06-22 | 1981-06-22 | |
| US276223 | 1981-06-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU8505182A AU8505182A (en) | 1983-01-06 |
| AU539531B2 true AU539531B2 (en) | 1984-10-04 |
Family
ID=23055717
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU85051/82A Ceased AU539531B2 (en) | 1981-06-22 | 1982-06-21 | Immunochemical assay for prostatic acid phosphatase |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0068291B1 (en) |
| JP (1) | JPS585661A (en) |
| AT (1) | ATE16407T1 (en) |
| AU (1) | AU539531B2 (en) |
| CA (1) | CA1185525A (en) |
| DE (1) | DE3267265D1 (en) |
| DK (1) | DK261282A (en) |
| ES (1) | ES8307378A1 (en) |
| FI (1) | FI821821A7 (en) |
| NO (1) | NO822067L (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4690890A (en) * | 1984-04-04 | 1987-09-01 | Cetus Corporation | Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay |
| US6942862B2 (en) | 1996-04-01 | 2005-09-13 | University Of Washington | Methods and compositions to generate immunity in humans against self tumor antigens by immunization with homologous foreign proteins |
| CN103808712B (en) * | 2012-11-05 | 2017-12-19 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of liquid reagent box and detection method for acid phosphatase detection |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4048298A (en) * | 1975-02-25 | 1977-09-13 | Rohm And Haas Company | Solid phase double-antibody radioimmunoassay procedure |
| US4224406A (en) * | 1978-02-27 | 1980-09-23 | Hoffmann-La Roche Inc. | Immunochemical LDH1 assay |
| US4260678A (en) * | 1979-02-23 | 1981-04-07 | Corning Glass Works | Determining creatine kinase isoenzmes via immobilized antibody-isoenzyme complexes |
| DE2952478A1 (en) * | 1979-12-27 | 1981-07-30 | Merck Patent Gmbh, 6100 Darmstadt | METHOD AND MEANS FOR THE IMMUNOLOGICAL DETERMINATION OF ENZYMES |
-
1982
- 1982-05-17 CA CA000403059A patent/CA1185525A/en not_active Expired
- 1982-05-21 FI FI821821A patent/FI821821A7/en not_active Application Discontinuation
- 1982-06-10 DK DK261282A patent/DK261282A/en not_active Application Discontinuation
- 1982-06-15 EP EP82105246A patent/EP0068291B1/en not_active Expired
- 1982-06-15 DE DE8282105246T patent/DE3267265D1/en not_active Expired
- 1982-06-15 AT AT82105246T patent/ATE16407T1/en not_active IP Right Cessation
- 1982-06-21 NO NO822067A patent/NO822067L/en unknown
- 1982-06-21 AU AU85051/82A patent/AU539531B2/en not_active Ceased
- 1982-06-21 ES ES513286A patent/ES8307378A1/en not_active Expired
- 1982-06-22 JP JP57107519A patent/JPS585661A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| FI821821A7 (en) | 1982-12-23 |
| AU8505182A (en) | 1983-01-06 |
| DK261282A (en) | 1982-12-23 |
| ES513286A0 (en) | 1983-07-01 |
| EP0068291A1 (en) | 1983-01-05 |
| NO822067L (en) | 1982-12-23 |
| FI821821A0 (en) | 1982-05-21 |
| DE3267265D1 (en) | 1985-12-12 |
| JPS585661A (en) | 1983-01-13 |
| CA1185525A (en) | 1985-04-16 |
| ATE16407T1 (en) | 1985-11-15 |
| EP0068291B1 (en) | 1985-11-06 |
| ES8307378A1 (en) | 1983-07-01 |
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