AU675289B2 - Method for the determination of hemoglobin adducts - Google Patents

Description

1 Method for the Determination of Hemoglobin Adducts Background of the Invention The level of glycation of certain circulating proteins, e.g. hemoglobin, can be used to monitor the average blood glucose because glycation is a non-enzymatic, slow and continuous reaction that is primarily dependent on the ambient glucose concentration to which the hemoglobin is exposed during its residence time in the circulatory system.

These two factors, glucose concentration and residence time, translate in vivo to the degree and duration of increased blood glucose concentration (hyperglycemia). Thus, when the blood glucose level is elevated, as it is in diabetic people whose diabetes is not well controlled, increased amounts of glycated hemoglobin are formed. The amount of glycated hemoglobin in an individual's blood reflects the average blood glucose concentration to which hemoglobin has been exposed during its life in the circulatory system. This period is about 100 days, so the determination of the concentration of glycated hemoo IN:1LIBW102261:GSA uI ls

C

2 globin can provide an historical picture of the individual's blood glucose profile.

Methods described for the measurement of glycated hemoglobin include chromatography on an ion exchange column or boronate affinity columns, HPLC and agarose gel electrophoresis. Each of these techniques has drawbacks with respect to complexity, costly instrumentation, accuracy or variability.

Immuno techniques are available whereby monoclonal 10 antibodies have been developed to react with epitopes of the glycated hemoglobin, i.e. that derivative of hemoglobin formed by the nonenzymatic reaction of glucose with reactive amine groups on the hemoglobin protein, and facilitate the determination of glycated hemoglobin concentration by standard immunochemistry techniques such as ELISA or a latex agglutination assay. Such an agglutination assay is disclosed in U.S. Patent 4,970,171 wherein there is described an immunoassay for glycated hemoglobin, e.g. HbAlc, a S: 20 variety of hemoglobin with glycation at the P subunit in a blood sample, which involves the steps of: a) treating the blood sample with a thiocyanate salt capable of denaturing the hemoglobin contained therein and an oxidant capable of converting the hemoglobin in the treated blood sample to the met-hemoglobin form; MSE #1838.1 -r III b) assaying the treated sample for met-hemoglobin as representing the amount of total hemoglobin in the sample; c) assaying the denatured, oxidized blood sample by immunoassay for the amount of denatured form of the particular hemoglobin derivative being sought; and d) calculating the relative amount of hemoglobin that is in the form of the hemoglobin derivative being sought compared to the total amount of hemoglobin in the test sample.

This glycated protein immunoassay was found to have a latent defect which affected its accuracy, which defect is based on the discovery that the response of a given concentration of glycated hemoglobin is not only dependent on its concentration but is also dependent on the total hemoglobin concentration. It has been discovered that there is an inverse relationship between the apparent glycated hemoglobin concentration and the total hemoglobin concentration, i.e. when the total hemoglobin concentration is high, the apparent go.o•i S" glycated hemoglobin concentration is less than expected and when total hemoglobin concentration is low the apparent glycated hemoglobin concentration is higher than expected. The same phenomena is observed in the course of determining the concentration of other hemoglobin adducts by equilibrium analytical methods in which the equilibrium of the dimeric form and tetrameric form of the hemoglobin molecule is not perturbed by the MSE #1838.1 4 chemistry. Immuno, enzymatic and chemical analytical methods are regarded as equilibrium methods whereas chromatographic and pepsin digestion methods are not. The present invention is predicated on the discovery of the underlying reason for this effect and the development of a method to overcome it.

Summary of the Invention The present invention is an improved method for determination of haemoglobin adducts, e.g. haemoglobin-acetaldehyde; haemoglobin-urea; haemoglobin-aspirin; haemoglobin-glucose-6-phosphate; haemoglobin-glucose-1,6-diphosphate; haemoglobinglutathion and haemoglobin-glycation end products, in a blood sample which method comprises the steps of: a) assaying the blood sample for the total amount of haemoglobin present therein; b) assaying the blood sample for the amount of haemoglobin adduct present therein; c) normalising the measurement from the haemoglobin adduct assay to the amount of total haemoglobin in the sample; and S. 15 d) dividing the normalised haemoglobin adduct concentration by the total haemoglobin concentration to obtain the corrected concentration of haemoglobin adduct.

According to a first embodiment of this invention, there is provided a method for the determination of a particular haemoglobin adduct in a blood sample which method comprises the steps of: 20 a) assaying the blood sample for the total amount of haemoglobin present therein; b) assaying the blood sample by an assay technique which is subject to bias based on S the total amount of haemoglobin in the blood sample and which is specific for the haemoglobin adduct whose concentration is being determined; c) normalising the measurement of the haemoglobin adduct to the total amount of ooo 25 haemoglobin in the sample; and d) dividing the normalised haemoglobin adduct concentration by the t'tal haemoglobin copcentration to obtain the corrected concentration of the haemoglobin adduct.

According to a second embodiment of this invention, there is provided a method for the determination of HbAlc in a blood sample which comprises the steps of: a) treating the blood sample with a denaturant capable of denaturing substantially all of the haemoglobin in the sample; b) assaying the denatured blood sample for total amount of haemoglobin present therein; c) assaying the denatured blood sample by an immunoassay technique which is specific for HbAlc; d) normalising the HbAlc measurement from the immunoassay to the amount of total haemoglobin in the sample; and [N:\LIBVV]00639:TCW ~ru I rr~l~- I- I- 4A e) dividing the normalised HbAlc concentration by the total haemoglobin concentration to obtain the corrected HIbAlc concentration.

According to a third embodiment of this invention, there is provided a device for determining the concentration of a haemoglobin adduct in a blood sample which comprises: a) means for determining the total amount of haemoglobin in the blood sample; b) means for determining the concentration of the haemoglobin adduct; c) means for normalising the measurement of the haemoglobin in the sample; and d) means for dividing the normalised haemoglobin adduct concentration by the total haemoglobin concentration to obtain the corrected concentration of the haemoglobin adduct.

*o See e so [N\LIBVVOO639:TCW Irr k--rllll 5 Brief Description of the Drawings Fig. 1 is a standard curve of an immunoassay for hemoglobin Alec. The curve was generated using six calibrators having different percentages of HbAlc but with the same hemoglobin concentration of 14 g/dL.

Fig. 2 illustrates the dependence of the observed percentage of HbAlc in terms of total hemoglobin concentration as determined by the immunoassay. It also shows the second order polynomial data fitting of 0 the observed percentage of HbAlc at different HbAlc levels.

Fig. 3 shows the a. coefficients at different levels of HbAlc. It also illustrates the linear data fit of the a 2 coefficients. The intercept of the linear fit is the I, coefficient and the slope of the linear fit is the S 2 coefficient.

*t Fig. 4 shows the a, coefficients at different levels of HbAlc. It also shows the linear data fit of the a, coefficients. The intercept of the linear fit is the I, coefficient and the slope of the linear fit •is the S, coefficient.

Description of the Invention Although the normalization procedure can be applied to any assay which, unlike chromatography, does not completely dissociate the hemoglobin molecule, the present invention is particularly useful for the MSE #1838.1 6 determination of glycated hemoglobin, e.g. HbAlc, by immunoassay techniques. Other pre-equilibrium assay methods which may be used include enzymatic, e.g. those involving the use of oxidases, reductases or phosphatases that can interact with the glucose, glucose-6phosphate or glucose-1,6-diphosphate in the adduct; and chemical, e.g. those involving chemical reactions that can oxidize, reduce or hydrolyze the adduct. More specifically, the glucose portion of the hemoglobinglucose adduct can be liberated as fural by acid treatment. The is then reacted with thiobarbituric acid to form a colored complex. The color intensity of the complex is proportional to the concentration of the hemoglobin- 15 glucose adduct present in the sample. Another example of chemical determination is the analysis of the adduct formed by acetaldehyde and hemoglobin. Acetaldehyde, the first metabolite of ethanol, has been shown to form .:..adducts with hemoglobin and the measurement of hemoglobin associated acetaldehyde has been reported to distinguish between drinking and non-drinking individuals. The acetaldehyde in the adduct can be hydrolyzed and then reacted with 1,3-cyclohexandeione to form a fluorophore which is analyzed quantitatively. The 25 fluorophore's concentration is proportional to the concentration of the hemoglobin-acetaldehyde adduct present in the sample. These methods for the determination of the hemoglobin adduct typically do not require denaturation of the hemoglobin.

The present invention is based upon the fact that native hemoglobin exists as an equilibrium of tetrameric MSE #1838.1 7 a and p chains (a2 P2) and the dimeric form (ap).

These a and p chains are each about 143 amino acids in length. At the end of the p subunit there is a valine unit which is part of the hemoglobin Alc epitope and can react with glucose. In a mixture of native hemoglobin and hemoglobin adducts (as in blood) there exists, in the case of glycated hemoglobin, a mixture of un-glycated tetramers, un-glycated dimers, monoglycated tetramers and mono-glycated dimers. All of the glycated components will have different immuno, enzymatic or chemical reactivity; the glycated dimers have the highest reactivity since their glycation sites are :exposed and accessible to interaction with the reactant.

The glycated tetramers have much lower reactivity because in the tetrameric configuration the glycated site is sterically hindered. When the total concentration of hemoglobin in the sample increases, the :i equilibrium between the tetrameric form of glycated hemoglobin and its dimeric form is shifted towards the tetrameric form because at higher concentrations, the associated, tetrameric form is thermodynamically favored. The combination of this equilibrium,, shift and the differences in reactivity between the tetrameric and dimeric forms of glycated hemoglobin with the 25 antibody reagent cause the assay to be affected by the total amount of hemoglobin present in the blood sample.

In the clinical setting, this "hemoglobin dependency effect", which is a bias based on the total amount of hemoglobin present in the sample, should be taken into account when the concentration of the hemoglobin adduct is being determined.

MSE #1838.1 8 Depending on the hemoglobin adduct whose presence or concentration is being sought and the particular method used in its assay, it may be neceE ery to expose the reactive site of the hemoglobin adduct by denaturation. The term denaturation, as used herein, is intended to mean any treatment which will expose the reactive site to the analytical reagent selected for use without disrupting the equilibrium between the dimeric and tetrameric hemoglobin chains. Suitable denaturation techniques include treating the sample S. with a high salt concentration; low or high pH; a S. choatropic reagent such as thiocyanate, guanidinium, urea or a surfactant such as a non-ionic, cationic or *anionic detergent. All of these treatments cause the 15 disruption to some degree of the hydrogen bonding of the hemoglobin molecule thereby exposing the reactive site. These treatments do not cause the complete dissociation of the hemoglobin and, therefore, do not alter the aforementioned eqvlibrium. Proteolytic digestion or chromatography would not be suitable for use in conjunction with the present method since it will disrupt this equilibrium. The determination of glycated hemoglobin, e.g. HbAlc, will typically involve •denaturation of the hemoglobin, especially when an 25 immunoassay technique is to be employed.

The method of the present invention, in which an immunoassay and a glycated hemoglobin are chosen for purposes of illustration, is carried out as follows: In the first step, the hemoglobin is denatured in order that the glycated N-terminal peptide residue MSE #1838.1 bl 9 becomes available for antibody binding. In United States Patent 4,658,022 there are described numerous techniques for protein denaturation inclucing treatment of the protein by physical means such as heat, sonijation, high and low pH and chemical denaturation by digestion or interaction with a chaotropic agent such as guanidine, urea or a detergent such as sodium dodecylsulfate. In a preferred technique, the protein is denatured to expose the peptide epitope for antibody bonding by treating the blood sample with a thiocyanate salt and an oxidant as disclosed in U.S. Patent 4,970,171 incorporated herein by reference. The :technique taught in tnis patent oxidizes the hemoglobin to met-hemoglobin which is converted to thiocyan-methemoglobin as the stable color component, i.e. the color intensity is correlated to the concentration of the hemoglobin. The resulting thiocyan-met-hemoglobin serves as the basis for measuring total sample hemoglobin with the denatured form of the particular hemoglobin derivative serving as the analyte in the immunoassay part of the procedure. The thiocyanate salt is selected from those salts which upon ionization provide the thiocyanate anion (SCN-) to render the hemoglobin suitable for detection in the immunoassay.

Ammonium, sodium and potassium are suitable countercations. Lithium thiocyanate provides faster denaturation as well as expedited lysing of red blood cells in the blood sample being tested. The oxidant, which can be essentially any inorganic oxidizing agent, converts the native hemoglobin ferrous ion to its ferric methemoglobin form. Suitable oxidants include ferricyanide, MSE #1838.1 10 iodate, chlorate, bromate, chromate, hypochlorite, iodate, periodate and peroxide.

Met-hemoglobin concentration is conveniently determined by measuring its characteristic absorbance at 540 nanometers using a conventional spectrophotometer such as the HP 8450A spectrophotometer or its equivalent.

Alternatively, one can use the Drabkin's procedure as described in National Commission for Clinical Laboratory Standards of the United States, Proposed Standard ."10 After denaturation, the blood sample is assayed for total met-hemoglobin and for the hemoglobin adduct of interest. This is most conveniently accomplished by immunoassay using monoclonal antibodies developed to bind specifically to the epitope that characterizes the hemoglobin adduct, e.g. hemoglobin Alc, in the denatured protein. The particular immunoassay technique and format, as well as the labeling approach and detection signal generated, is not critical. Radioisotopic or 2.o. enzyme labeled (ELISA) techniques can be used. A particle agglutination inhibition immunoassay based on the specific interaction of an antibody particle reagent and an agglutinator reagent is particularly useful. The antibody particle reagent comprises the monoclonal antibody, or a fragment thereof, bound to a water suspensible particle, typically a polystyrene or other latex. The agglutinator comprises a plurality of epitopic binding sites for the antibody reagent. In the absence of analyte, the antibody particle and agglutinator bind to one another to form a light MSE #1838.1 11 scattering complex that can be quantified by turbidimetric measurement. In che presence of increasing amounts of analyte, the turbidity of the solution decreases as antibody particles become bound to the analyte and cannot bind to the agglutinator which is typically a polymer backbone to which is atta-hed a number of organic moieties, such as peptide residues, which define the epitope that characterizes the hemoglobin derivative of interest. When hemoglobin Alc is to be determined, the epitope comprises the glycated peptide residues of a few amino acid units corresponding to the sequence of the glycated N-terminal residue in hemoglobin Alc.

After the concentration of total hemoglobin and the hemoglobin derivative are determined, the percentage of the hemoglobin adduct as a portion of total hemo- :I globin is calculated. This determination, as taught in the prior art, is carried out by simply dividing the hemoglobin derivative concentration by the concentration 20 of total hemoglobin to arrive at the percentage value of the hemoglobin adduct. This calculation does not provide a totally accurate picture, however, due to the previously mentioned hemoglobin dependency effect. It is an object of the present invention to provide a procedure for factoring the hemoglobin dependency effect out of the determination of the hemoglobin derivative to thereby provide a more accurate procedure for determining the concentration of the hemoglobin derivative under consideration. This improved procedure involves no.minalizing the concentration of the hemoglobin I-rivative as determined by the assay. This is MSE #1838.1 12accomplished by deducing the true concentration of hemoglobin derivative from its reactivity and the total concentration of hemoglobin, both of which values are determined during the assay. The normalization procedure is carried out in 5 steps. These steps are: A. Creating a cplibration curve relating the concentration of the hemoglobin adduct as determined by a reference procedure, such as HPLC, which is not subject to the hemoglobin effect to the immuno reaction response by using calibrator blood samples containing a normalized concentration of hemoglobin and different concentrations of the hemoglobin adduct; B. Preparing a series of blood samples containing different concentrations of hemoglobin but the same percentage of hemoglobin adduct in each series, determining the immunoreaction response from each sample and obtaining the observed concentration of hemoglobin adduct for each sample from the calibration curve generated in Step A; C. Creating a series of polynomial (nh order) •fitted curves relating the observed concentration S•of hemoglobin adduct from each series as determined in Step B to the concentration of hemoglobin in each sample in the series, obtaining the polynomial curve fitting coefficients a-2 for each curve and repeating the process for the whole series of curves; MSE #1838.1 13 D. Obtaining the linear curve fitting relating the polynomial curve fitting coefficient an for the series of curves generated in Step C to the percentage of hemoglobin adduct at the normalized concentration of hemoglobin and obtaining the slope (Sn) and intercept (In) of the linear curve fit; E. Obtaining the Sn- S and In-i, In-2 I1 values from the polynomial curve fitting coefficient a, respectively by the 10 procedure of step D; and a F. Obtaining the corrected hemoglobin adduct concentration by relating the observed hemoglobin adduct concentration to hemoglobin concentration by solving the equation: 15 Hb,, (Hb"--14n- 2 2 -l14~ 2 where: e 20 Hba, is the corrected concentration of the hemoglobin adduct whose concentration is being sought; is the observed concentration of hemoglobin adduct as determined by immunoassay; Hb is the total hemoglobin concentration in terms of g/dL; MSE #1838.1 14 I, and S S, are experimentally determined coefficients; and where 14 represents 14 g/dL hemoglobin selected as the normalized concentration. Normalized concentration, as used herein means a fixed concentration of hemoglobin.

Any concentration can be selected as the normalized concentration, however, 14 g/dL is preferred for use in this procedure because the majority of clinical blood specimens will contain this concentration of hemoglobin.

10 The advantage in this is that, if the specimen's .hemoglobin concentration is equal to the normalized concentration, no correction is required, since, in that case, the corrected ccncentration is equal to the observed concentration.

The method of practicing the present invention is further illustrated by the following example in which HbAlc is the hemoglobin adduct of interest. In this example the second order polynomial is selected for illustration purposes. One can use a linear, 2 order or higher order polynomial curve fit in this procedure.

However, the linear curve fit will not correct the •curvature of the results and the algorithm for higher order polynomials will be more complicated and carry more coefficients, i.e. the linear fit will require coefficients Ii and the second order polynomial will require coefficients I, S, and S 2 while the third order polynomial will require coefficients Ii, I2, I 3 SI, S 2 and S 3 MSE #1838.1 15

EXAMPLE

A. Six calibrators were prepared containing 8.93%, 10.97% and 12.54% hemoglobin Alc respectively as determined by HPLC. The total hemoglobin concentration in each calibrator blood sample was 14 g/dL. The immunoreaction response of each calibrator was measured by the latex agglutination technique previously described with the results being set out in Table I.

0 TABLE I S. %Alc 14 g/dL Alc (mM) mA 0 0 827.9 108.5 670.5 5.93% 257.4 440.2 7.97% 345.9 321.7 8.93% 387.6 271.6 10.97% 476.1 212.3 12.54% 544.2 173.4 20 The immunoreaction responses were used to generate the calibration curve of Fig. 1 with the immunoreaction responses being used as the Y values and the reference Alc concentrations used as the X values. In this example, the immunoreaction response is represented by mA (540 nm, 168-28) which is the difference in milliabsorbance reading at a wavelength of 540 nanometers between 28 and 168 seconds after start of the reaction.

B. The next step was to prepare six groups of samples containing 5.93%, 7.97%, 8.93%, 10.97% MSE #1838.1 I I 16 and 12.54% Alc (corresponding to the HbAlc levels found by the reference method in step A) each of which group having 5 or more levels of hemoglobin concentration between 9 and 20 g/dL. These samples were prepared from packed human red blood cells and human plasma.

Their immunoreaction responses were measured by the latex agglutination method previously described and from the immunoreaction responses, the Alc concentrations of these samples were calculated using the calibration curve generated in Step A. The sample concentrations are shown in Table II as observed Alc values. The procedure for measuring HbAlc is described in detail in U.S. Patent 4,847,209 as well as how .M HbAlc relates to HbAlc. The teachings of this patent 15 are incorporated herein by reference.

C. For each group of samples in Step B, the observed Alc values were plotted against the hemoglobin concentration (Fig. Using the observed Alc values as Y values and the hemoglobin concentration as X values (Table II), the data can be fitted with the polynomial curve fit using a commercial curve fitting program such as SlideWrite@ from Advance Graphic Software Inc. or Sigmaplot© from Jandel Scientific.

The data can be fitted with any order polynomial.

For the group of 2.5% samples, the observed Alc values of 2.28, 2.43, 2.57, 2.43, 2.75 and 2.5 were used as the Y values and the hemoglobin concentration of 21.9, 17.5, 14, 14, 12.1, 10.4 were used as the X values. After the 2 n order polynomial curve fitting of these data, the equation Y=-0.0011617*X'+0.02542*- X+2.490 was generated for the group of samples.

MSE #1838.1 I 17 For the group of 5.93% samples, the observed Alc values of 5.17, 5.58, 5.79, 6.06, 6.35 and 6.45 were used as the Y values and the hemoglobin concentration of 19.9, 16.3, 14, 13.2, 11.7 and 10.3 were used as the X values. After the 2 n d order polynomial curve fitting of these data, the equation Y=0.004478*X 2 -0.2747*- X+8.859 was generated for this group of samples.

For the group of 7.97% samples, the observed Alc values of 7.05, 7.88, 8.05, 8.26, 8.87 and 9.08 were 10 used as the Y values and the hemoglobin concentration of 20.4, 16.8, 14, 13.4 11.6 and 10.4 were used as the X values. After the 2 n" order polynomial curve fitting of these data, the equation Y=0.006158*X 2 -0.3860*- X+12.419 was generated for this group of samples.

For the group of 8.93% samples, the observed Alc 'values of 7.92, 8.60, 9.33, 9.26, 9.92 and 10.58 were used as the Y values and the hemoglobin concentration of 21.9, 17.1, 14.2, 14, 12.3 and 10.8 were used as the X values. After the 2 d order polynomial curve fitting of these data, the equation Y=0.017231*X 2 -0.7985*- X+17.158 was generated for this group of samples.

SFor the group of 10.97% samples, the observed Alc values of 9.72, 10.94, 11.02, 12.14, 12.96 and 14.13 were used as the Y values and the hemoglobin concentration of 19.7, 15.2, 14, 12, 10.4, 8.7 were used as the X values. After the 2 n order polynomial curve fitting of these data, the equation Y=0.01644*-

X

2 -1.14462*X+11.064 was generated for this group of samples.

MSE #1838.1 18 For the group of 12.54% samples, the observed Alc values of 10.13, 11.97, 12.44, 13.08, 14.46 and 16.01 were used as the Y values and the hemoglobin concentration of 20.9, 16.2, 14, 13, 11 and 9.2 were used as the X values. After the 2 "d order polynomial curve fitting of these data, the equation Y=0.02734*-

X

2 -1.3086*X+25.608 was generated for this group of samples.

In these second order equations; -0.001617, 10 0.004478, 0.006158, 0.017231, 0.02644 and 0.02734 are the a, coefficients and 0.02542, -0.2747, -0.386, -0.7985, -1.1462 and -1.3086 are the a, coefficients generated from the curve fitting steps. The procedures 0 of steps D and E require the use of these a, and a 2 coefficients for further calculation.

*000 D. The a 1 values, 0.02542, -0.2747, -0.3860, -0.7985, -1.1462 and -1.3086 were used as the Y values and plotted against the Alc values of 2.5, 5.93, '0 7.97, 8.93, 10.97 and 12.54 as the X values to prepare 20 Fig. 3. The data were fit with a linear curve fit using the Slidewrite© curve fitting program. This 0. Go linear curve fitting gives the result of: 0 0 Y -0.141*X 0.505 where, -0.141 is the S 1 coefficient and 0.505 is the I, coefficient.

E. The a 2 values -0.001617, 0.004478, 0.006158, 0.017231, 0.02644 and 0.02734 were used as the Y values MSE #1838.1 19 and plotted against the Alc values of 2.5, 5.93, 7.97, 8.93, 10.97 and 12.54 as the X values to prepare Fig. 4. The data were fit with a linear curve fit to give the result of: Y 0.0032*X -0.0129 where 0.0032 is the S, coefficient and 00.0129 is the I, coefficient.

F. The corrected Alc concentration was obtained .by solving the equation: :10 Alc (Alc'-(Hb 2

*S

2 (Equation I) where: Alc' is the observed HbAlc as determined by the immunoassay; SHb is the total hemoglobin concentration in terms of g/dL; I, S= and S, are experimentally determined coefficients, and 14 is 14 g/dL hemoglobin selected as the normalized concentration.

MSE #1838.1 20 When the concentration of hc-oglobin is further away from the normalized concentration, more bias of the HbAlc value from the reference value HPLC value) is observed. Therefore, it is advantageous to set the normalized concentration to the concentration of hemoglobin that is most commonly encountered in the blood sample being examined to provide a test having the least bias. This correction method can be used with various normalized concentrations, e.g. 13 g/dL, 10 14 g/dL, 15 g/dL, etc. However, since the majority of blood specimens will have a hemoglobin concentration of 14 g/dL, it is advantageous to select this level as the normalized concentration.

In the foregoing example there is described the determination of the hemoglobin Alc epitope, however, the technique of the present invention is equally applicable to the determination of other hemoglobin derivatives, such as those having other glycated amine Sepitopes along the a and P subunits. Likewise, the present method is applicable to assay methods other than immunoassay which do not completely dissociate the S" hemoglobin. These methods include chemical and enzymatic assays.

Referring to Table II which reports the results of this experiment, the bias difference between the observed value and the reference value) of the uncorrected results ranged from 28.7% to After correction with the algorithm (Equation I) and the above coefficients, the bias were reduced to 7.0 to MSE #1838.1 _q

O

O

0 n Hemoglobin Correction for HbAlc Reference Alc Observed Hb Observed Values Concentrations Alc Values TABLE II Determined by Latex Bound Antibody Agglutination Method Bias from Alc After Bias from Reference Reference Valuer Correction Values After Correction 21.9 2.28 -11.3 2.46 -4.4 17.5 2.43 5.4 2.43 -5.6 14 2.57 0.0 2.57 0.0 14 2.43 5.4 2.43 -5.4 12.1 2./3 6.2 2.75 10.4 2.5 2.7 2.60 5.93% 19.9 5.17 -10.7 5.90 16.3 5.58 3.6 5.92 2.2 14 5.79 0.0 5.79 0 0 13.2 6.06 4.7 5.93 2.4 11.7 6.35 9.7 5.95 2.7 10.3 6.45 11.4 5.80 0.2 1.97% 20.4 7.05 -12.4 8.30 3.1 16.8 7.88 2.1 8.60 6.8 14 8.05 0.0 8.05 0.0 13.4 8.26 2.6 8.09 11.6 8.87 10.2 8.14 1.1 10.4 9.08 12.8 7.96 -1.1 8.93% 21.9 7.92 -14.5 9.56 3.3 17.1 8.6 7.1 9.50 2.6 14.2 9.33 0.8 9.40 14 9.26 0.0 9.26 0.0 12.3 9.92 7.1 9.30 10.8 10.58 14.3 9.33 0.8 10.97% 19.7 9.72 -11.8 11.49 4.3 15.2 10.94 0.7 11.44 3.8 14 11.02 0.0 11.02 0.0 12 12.14 10.2 11.19 10.4 12.96 17.6 11.12 8.7 14.13 28.2 11.20 1.6 12.54% %Bias Calculated 20.9 16.2 14 13 11 9.2 Against 10.13 11.97 12.44 13.08 14.46 16.01 14 g/dL Values -18.6 3.8 0.0 5.1 16.2 28.7 12.23 12.98 12.44 12.55 12.69 12.87 -1.7 4.4 0.0 0.9

I

22 The normalization for hemoglobin concentration step of the present invention can be carried out by means of a pre-programmed computer memory which may be in the form of a microchip. Incorporating the microchip into a known device for the determination of a preselected hemoglobin adduct, such as that described in Diabetes Care, D.G. Marr:ro, et al 15/8 1045-1049 (1992), provides a device comprising a) means for determining the total amount of 10 hemoglobin in the blood sample; oe Sb) means for carrying out an assay of the hemoglobin adduct; c) means for normalizing the measurement of the hemoglobin adduct to the total amount of hemoglobin 15 in the sample; and d) means for dividing the normalized hemoglobin adduct concentration by the total hemoglobin concentration to obtain the corrected concentra- S" tion of the hemoglobin adduct.

When the hemoglobin adduct involved and/or the assay method are such that denaturation of the hemoglobin are required, the device will also include means for denaturing the hemoglobin.

The instrument contains a memory which is preprogrammed to do all of the calculations necessary for normalizing the measured concentration of the hemoglobin MSE #1838.1 -ill~C1 i_ f 23 adduct to the total amount of hemoglobin in the blood sample.

The mathematical equation, e.g. Equation I and the I and S coefficients can be programmed into and stored in the memory of a calculation device such as a calculator, computer or computer that has been interfaced with a clinical analyzer capable of determining the total concentration of hemoglobin and that of the hemoglobin adduct. For each clinical sample, the 10 observed concentration of hemoglobin adduct and total hemoglobin concentration is determined by running the assay. The corrected hemoglobin adduct concentration is then determined either manually using the calculator or by means of the pre-programmed computer. Alternatively the asults can be obtained directly from a computer that has been interfaced with the clinical analyzer.

e o MSE #1838.1

P

Claims (27)

1. A method for the determination of a particular haemoglobin adduct in a blood sample which method comprises the steps of: a) assaying the blood sample for the total amount of haemoglobin present therein; b) assaying the blood sample by an assay technique which is subject to bias based on the total amount of haemoglobin ii: the blood sample and which is specific for the haemoglobin adduct whose concentration is being determined; c) normalising the measurement of the haemoglobin adduct to tle total amount of haemoglobin in the sample; and d) dividing the normalised haemoglobin adduct concentration by the total haemoglobin concentration to obtain the corrected concentration of the haemoglobin adduct.

2. The method of claim 1, wherein the normalisation step is accomplished by: A. creating a calibration curve relating the concentration of the haemoglobin adduct as determined by a reference procedure which is not subject to bias based on the total amount of haemoglobin in the blood sample to the response obtained by analysing calibrator blood samples for the haemoglobin adrluct using a method which is subject to bias based on the total amount of haemoglobin in thu blood sample; B. providing a seres of blood samples containing different concentrations of 20 haemoglobin with the ,amne concentration of haemoglobin adduct in each sample, determining the concentration of the adduct by the method which is subject to bias and obtaining the observed concentration of haemoglobin adduct for each sample from the calibration curve generated in step A: C. creating a series of polynomial (n order) fitted curves relating the observed 25 concentration of haemoglobin adduct from each series as determined in step B to the concentration of haemoglobin in each sample in the series, obtaining the polynomial curve fitting coefficients (an, an.2 a) for each curve and repeating the process for the whole series of curves; D. obtaining the linear curve fitting relating the polynomial curve fitting coefficient an for the series of curves generated ii step C to the percentage of haemoglobin at a normalised concentration of haemoglobin and obtaining the slope (Sn) and intercept (In) of the linear curve fit; E. obtaining the Sn- 2 SI and In-2...Ii values from the polynomial curve fitting coefficient a,. 1 a, 2 a respectively; and F. obtaining the corrected haemoglobin adduct concentration by relating th: observed haemoglobin adduct concentration to the observe total haemoglobin concentration by solving the equation (Hb" I, (Hb-2 n-2 (Hb N) I,) Hb Hb N 2 N)*S 1) where: :\L4BCIOC248:JOC 25 of a Hbad is the corrected concentration of the haemoglobin adduct, the concentration of which is being sought; Hbad. is the observed concentration in terms of g/dL; I and 1 are experimentally determined coefficients; and N represents the normalised concentration of haemoglobin in g/dL.

3. The method of claim 2, wherein the normalised concentration of haemoglobin is 14

4. The method of any one of claims 1 to 3, wherein the haemoglobin adduct is haemoglobin-acetaldehyde; haemoglobin-urea; haemoglobin-aspirin; haemoglobin- glucose-6-phosphate; haemoglobin-glucose-1,6-diphosphate or haemoglobin-glutathion.

The method of claim 4, wherein the haemoglobin adduct is glycated haemoglobin.

6. The method of claim 5, wherein the glycated haemoglobin is HbAlc.

7. The method of any one of claims 1 to 6, wherein the observed adduct concentration is determined by immunoassay, chemical or enzymatic techniques.

8. The method of claim 7, wherein there is used an enzymatic technique in which the enzyme is a oxidase, reductase or phosphatase.

9. The method of claim 7, wherein there is used a chemical assay involving treating the haemoglobin adduct with 5-hydroxymethylfurfuryl and thiobarbituric acid to 20 form a coloured complex.

The method of claim 7, wherein the observed adduct concentration is determined by a latex/agglutination immunoassay.

11. The method of any one of claims 1 to 10 wherein the haemoglobin is denatured before the assay. 25

12. The method of claim 11, wherein the denaturation is accomplished by treating the blood sample with a high salt concentration, low or high pH, a chaotropic reagent or a surfactant.

13. The method of claim 12, wherein the denaturation reagent is a surfactant.

14. The method of claim 13, wherein the surfactant is a non-ionic, cationic or anionic detergent.

The method of any one of claims Ito 14, wherein the polynomial curves are of the second order.

16. A method for the determination of HbAlc in a blood sample which comprises the steps of: a) treating the blood sample with a denaturant capable of denaturing substantially all of the haemoglobin in the sample; b) assaying the denatured blood sample for total amount of haemoglobin present therein; c) assaying the denatured blood sample by an immunoassay technique which is specific for HbAlc; IN: LUBC0I0248:JOC 26 o d) normalising the HbAlc measurement from the immunoassay to the amount of total haemoglobin in the sample; and e) dividing the normalised HbAlc concentration by the total haemoglobin concentration to obtain the corrected HbAlc concentration.

17. The method of claim 16, wherein the HbAlc concentration is normalised by the steps of: A. creating a calibration curve relating the concentration of HbAlc as determined by a reference procedure which is not subject to bias based on the total amount of haemoglobin in the blood sample to the response obtained by analysing calibrator blood samples for HbAlc by immunoassay; B. providing a series of blood sample containing different concentration of haemoglobin with the same concentration of HbAlc in each sample, determining the concentration of HbAlc by immunoassay and obtaining the observed concentration of HbAlc for each sample from the calibration curve generated in step A; 15 C. creating a series of second order polynomial fitted curves relating the observed concentration of HbAlc from each series as determined in step B to the concentration of haemoglobin in each sample in the series, obtaining the polynomial curve fitting coefficients for each curve and repeating the process for the whole series of curves; D. obtaining the linear curve fitting relating the polynomial curve fitting 20 coefficient a, for the series of curves generated in step C to the percentage of haemoglobin at a normalised haemoglobin concentration of 14g/dL and obtaining the slope (Si) and intercept (1I) of the linear curve fit; E. Obtaining the S 2 and I1 values from the polynomial curve fitting coefficient a,; and 25 F. Obtaining the corrected HbAlc concentration by relating the observed HbAlc concentration to the observed total haemoglobin concentration by solving the equation (Alc'-(Hb 2 14 2 12 (Hb 14) I,) Alc ((Hb 2 14 2 S 2 (Hb 14) S, 1) where: Alc is the corrected HbAlc concentration: Alc' is the observed HbAlc concentration as determined by the immunoassay; Hb is the total haemoglobin concentration in terms of g/dL; 12, I1, S 2 and S, are experimentally determined coefficients, and 14 is 14g/dL haemoglobin selected as the normalised haemoglobin concentration.

18. A device for determining the concentration of a haemoglobin adduct in a blood sample which comprises: a) means for determining the total amount of haemoglobin in the blood sample; of b) means for determining the concentration a. the haemoglobin adduct; c) means for normalising the measurement of the haemoglobin in the sample; and IN:\LIBC100248.JOC 27 of 4 d) means for dividing the normalised haemoglobin adduct concentration by the total haemoglobin concentration to obtain the corrected concentration of the haemoglobin adduct.

19. The device of claim 18, wherein the means for normalising the measurement s of the haemoglobin adduct is a calculating device containing a memory in which the equation (Hb" (HHb"n N" n N) ad N (Hb 2 N"' 2 (Hb N) S, 1) and the I and S coefficients are preprogrammed.

The device of claim 19, wherein the second order polynomial equation is used.

21. The device of any one of claims 18 to 20, which also contains means frr contacting the haemoglobin in the blood sample with a denaturant.

22. The device of claim 21, wherein the denaturant is salt, a reagent which produces alow or high pH, a chaotropic reagent or a surfactant.

23. The device of claim 22, wherein the denaturant is a surfactant.

24. The device of claim 23, wherein the surfactant is a non-ionic, cationic or anionic detergent.

A method for the determination of a particular hemoglobin adduct in a blood sample, substantially as hereinbefore described with reference to the Example. 20

26. A method for the determination of HbAlc in a blood sample, substantially as hereinbefore described with reference to the Example.

27. A device for determining the concentration of a hemoglobin adduct in a blood se sample, substantially as hereinbefore described with reference to the Example. Dated 24 March, 1994 25 Miles Inc. Patent Attorneys for the Applicant/Nominated Person SPRUSON FERGUSON IN:\LIBCO00248:JOC 28 of 4 Method for the Determination of Haemoglobin Adducts Abstract Disclosed is an improvement to the method of determining the concentration of a haemoglobin adduct in a blood sample by the steps of denaturing the haemoglobin in the blood sample, assaying the blood sample for the total amount of haemoglobin, assaying the blood sample for the haemoglobin adduct, and dividing the haemoglobin adduct concentration by the total haemoglobin concentration. The improvement involves normalising the measurement of the haemoglobin adduct to the total amount of haemoglobin in the blood sample. The method of the invention comprises the steps of: a) assaying the blood sample for the total amount of haemoglobin present therein; b) assaying the blood sample by an assay technique which is subject to bias based on the total amount of haemoglobin in the blood sample and which is specific for the haemoglobin adduct whose concentration is being determined; 15 c) normalising the measurement of the haemoglobin adduct to the total amount of haemoglobin in the sample; and d) dividing the normalised haemoglobin adduct concentration by the total haemoglobin concentration to obtain the corrected concentration of the haemoglobin adduct. o No Figure. IN:\LIBMIO290 :JOC 1 of 1