AU701247B2 - Vaccine and method for treatment of chlamydial infections - Google Patents

Description

L re~k~~ddL--~Lls WO 95/12411 PCT/US94/12626 Vaccine and Method for Treatment of Chlamydial Infections Background of Invention Chlamydia trachomatis is the most prevalently sexually transmitted bacterial pathogen in the United States today. The complications resulting from chlamydial infections can be quite serious. Those infected may suffer from pelvic inflammatory disease, urethritis, urethral syndrome and urinary tract infections. It has also been confirmed that infection may result in spontaneous abortion in pregnant woman. In addition, chlamydial conjunctivitis and chlamydial pneumonia may occur in infants infected from their mothers as they pass through the birth canal.

Chlamydial infections in animals and humans are quite similar. The organism C. psittaci in animals primarily affects the mucosal epithelial cells of the eye and genital tract. After infection, a chronic carrier state typically develops with symptoms reoccurring during stress. Asymptomatic female animals carrying C.

psittaci in epithelial cells of the distal genital tract have been shown to infect their newborn during parturition.Chlamydial infection in sheep is an economically devastating disease in many countries. Ovine chlamydial abortion, also referred to as ovine enzootic abortion (OEA), results from infection by the C. psittaci pathogen.

This organism causes a necrotizing placentitis in sheep and consequent abortion of the lamb. Vaccines prepared from egg-grown Chlamydia psittaci inactivated with Formalin induced immunity in ewes against ovine chlamydial abortion. This vaccine and similar products have been used successfully for decades in sheep to protect agilinst OEA strains of the pathogen. Recently, however, the efficacy of this vaccine has been quite variable, with outbreaks of chlamyal OEA infection occurring in vaccinated flocks. Heterologous challenge experiments have indicated possible strain variation to be the cause.

More recently, protection of sheep against OEA has been demonstrated using a subcellular vaccine containing major outer membrane protein (MOMP) and a subfraction vaccine containing elementary bodies (EBs) from an OEA chlamydial strain (Tan et al., 1990, Infect. Immun., 58:3101-3108). Tan et al. used a modified procedure for isolating chlamydial outer membrane complexes (COMCs) to produce a subcellular vaccine highly enriched in undenatured MOMP. This preparation, given as a single dose containing 20 pg of protein, protected sheep against OEA. A single dose of a vaccine prepared from purified EBs, which contained 160 pg protein, also provided protection against OEA in sheep. Tan et al. identified MOMP C I I- 'M-f o? WO 95/12411 PCT/US94/12626 as the major protective component in OEA vaccines and suggested using a recombinant DNA approach to protect against OEA because they believed a 40 kDa MOMP antigen alone was sufficient. While the vaccine disclosed by Tan et al.

contained residual amounts of lipopolysaccharide (LPS), this component was not considered important in development of a vaccine against OEA chlamydial infections because serum containing antibodies against LPS did not confer protection in passive transfer experiments, and the complement-fixing antibodies thought to be directed against genus-specific epitopes of LPS did not correlate with protection against ovine abortion strains of C. psittaci.

Attempts to vaccinate against other strains of chlamydial infectiors in humans and in animals have been even less successful. Several vaccines prepared from attenuated-live or inactivated organisms are available for prevention of C.

psittaci infections. However, these vaccines have only resulted in reduction of symptom severity, not in the prevention of disease or elimination of the organism.

Vaccination against C. trachomatis in humans using killed, whole elementary bodies has proven to be somewhat protective, however, a delayed-type hypersensitivity has occurred in some cases, exacerbating the disease. Ocular administration of a 57 kDa heat shock protein has been shown to induce a mononuclear cellular inflammatory response in animals. In addition to the heat shock protein, the lipopolysaccharide (LPS) component of Chlamydia has also been shown to contribute to the pathogenesis of the ocular disease caused by the Chlamydia organisms.

While whole virus Chlamydia vaccines have been shown to be somewhat protective, they have also been shown to cause deleterious effects. This has led to the evaluation of individual components for use in a subunit or recombinant vaccine.

Polyclonal and monoclonal antibodies to the C. trachomatis major outer membrane protein (MOMP) have been shown to neutralize the ocular infectivity of this organism in a primate model. Oral immunization of monkeys with purified MOMP from C. trachomatis has only resulted in partial protection from subsequent ocular challenge. Due to its protective capacity and the ability of antibodies specific for MOMP to neutralize infectivity, research has focused on the identification of B- and T-cell epitopes on this protei,n.

It has now been found that the presentation of MOMP from Chlamydia organisms, either alone or in the context of elementary bodies or outer membrane complex, in combination with LPS, is an effective vaccine against chlamydial infections in animals. This combination is more efficient in the induction of a protective response against chlamydial infection than denatured MOMP alone.

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n 3 -3- Summary of Invention In one aspect, the present invention provides a vaccine for treatment of chlamydial infections comprising major outer membrane protein and lipopolysaccharide from a Chlamydia organism.

Accordingly there is provided by the present invention a vaccine for immunizing or treating an animal against infection by strains of Chlamydia psittaci to which said animal is susceptible, comprising: a prepar-:ion of major outer membrane protein, MOMP, from one or more of said strains of Chlamydia psittaci wherein said MOMP comprises one or more members selected from the group consisting essentially of: MOMP of molecular weight about 40 kDa which has been substantially purified by separation from whole cell lysates of one or more of said strains of Chlamydia psittaci; MOMP of molecular weight about 40 kDa designated MOMP-E, S 15 which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE; MOMP of molecular weight about 40 kDa designated MOMP-C, which has been prepared by: 20 treating whole cell lysates of EBs of said strains of Chlamydia psittaci with meglumine acetrizoate or diatrizoate, or sodium diatrizoate, or mixtures thereof and centrifugation to provide a purified EB subfraction; inactivating said EB subfraction; solubilizing and thereby removing non-MOMP proteins from said EB S: 25 subfraction by treatment with N-lauroyl-sarcosine and centrifugation whereby a sarcosyl-insoluble fraction remains consisting essentially of empty EBs with outer membranes, designated chlamydia outer membrane complexes, COMCs; extracting said MOMP-C from said COMCs fraction by treating said fraction with sodium dodecyl sulfate, SDS; and separating said MOMP-C from said SDS treated COMCs fraction and substantially purifying the same by means of chromatography; a preparation of lipopolysaccharide, LPS, from one or more of said strains of Chlamydia psittaci which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of polyacrylamide gel electrophoresis wherein a portion of said gel below about 6 kDa is removed and incubated and the resulting solution is purified to give said LPS; N:A\UlO0005:KWW iv ~1 7, k-u 3a a pharmaceutically acceptable carrier; and optionally, an adjuvant.

In another aspect, the present invention provides a method for treatment of chlamydial infections comprising administering tw an infected animal an effective amount of a vaccine comprising major outer membrane protein and lipopolysaccharide from a Chlamydia organism.

Accordingly there is also provided by the present invention a method for treating Chlainydia psittaci infections in an animal susceptible thereto comprising administering to said animal an effective amount of a vaccine comprising: a preparation of major outer membrane protein, MOMP, from one or more of said strains of Chlamydia psittaci wherein said MOMP comprises one or more members selected from the group consisting essentially of: MOMP of molecular weight about 40 kDa which has been 15 substantially purified by separation from whole cell lysates of one or more of said strains of Chlamydia psittaci; MOMP of molecular weight about 40 kDa designated MOMP-E, which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE; MOMP of molecular weight about 40 kDa designated MOMP-C, D which has been prepared by: treating whole cell lysates of EBs of said strains of Chlamydia psittaci with meglumine acetrizoate or diatrizoate, or sodium diatrizoate, or mixtures thereof 25 and centrifugation to provide a purified EB subfraction; inactivating said EB subfraction; solubilizing and thereby removing non-MOMP proteins from said EB subfraction by treatment with N-lauroyl-sarcosine and centrifugation whereby a sarcosyl-insoluble fraction remains consisting essentially of empty EBs with outer membranes, designated chlamydial outer membrane complexes, COMCs; extracting said MOMP-C from said COMCs fraction by treating said fraction with sodium dodecyl sulfate, SDS; and separating said MOMP-C from said SDS treated COMCs fraction and substantially purifying the same by means of chromatography; a preparation of lipopolysaccharide, LPS, from one or more of said strains of Chlamydia psittaci which has been separated from whole cell lysates of elementary R 7v% k,

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I -i I- 3bbodies, EBs, of said strains of Chlamydia psittaci by means of polyackylamide gel electrophoresis wherein a portion of said gel below about 6 kDa is removed and incubated and the resulting solution is purified to give said LPS; a pharmaceutically acceptable carrier; and optionally, an adjuvant.

In yet another aspect, the present invention provides a method for immunizing a healthy animal against cllamydial infections comprising administering to a healthy animal a vaccine comprising major outer membrane protein and lipopolysaccharide from a Chlamydia organism.

Accordingly there is also provided by the present invention a method for immunizing and treating an animal against infection by strains of Chlamydia psittaci to which said animal is susceptible, comprising: 4o 0 administering to a healthy said animal an immunogenically and therapeutically Sis effective amount of a vaccine comprising: a preparation of major outer membrane protein, MOMP, from one or more cf said strains of Chlamydia psittaci wherein said MOMP comprises one or more members selected from the group consisting essentially of: MOMP of molecular weight about 40 kDa which has been S 20 substantially purified by separation from whole cell lysates of one or more of said strains of Chlamydia psittaci; t(2) MOMP of molecular weight about 40 kDa designated MOMP-E, which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of sodium dodecyl sulfate polyacrylamide gel 25 electrophoresis, SDS-PAGE; S(3) MOMP of molecular weight about 40 kDa designated MOMP-C, which has been prepared by: treating whole cell lysates of EBs of said strains of Chlamydia psittaci with meglumine acetrizoate or diatrizoate, or sodium diatrizoate, or mixtures thereof and centrifugation to provide a purified EB subfraction; inactivating said EB subfraction; solubilizing and thereby removing non-MOMP proteins from said EB subfraction by treatment with N-lauroyl-sarcosine and centrifugation whereby a sarcosyl-insoluble fraction remains consisting essentially of empty EBs with outer membranes, designated chlamydial outer membrane complexes, COMCs; extracting said MOMP-C from said COMCs fraction by treating said fraction with sodium dodecyl sulfate, SDS; and (N ibz1OOOG5 KWW 3c separating said MOMP-C from said SDS treated COMCs fraction and substantially purifying the same by means of chromatography; a preparation of lipopolysaccharide, LPS, from one or more of said strains of Chlamydia psittaci which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of polyacrylamide gel electrophoresis wherein a portion of said gel below about 6 kDa is removed and incubated and the resulting solution is purified to give said LPS; a pharmaceutically acceptable carrier; and optionally, an adjuvant.

Detailed Description The present invention provides a vaccine for treatment and immunization against chlamydial infections in animals. The vaccine is comprised of major outer 15 membrane protein (MOMP) either alone in purified form or in the context of elementary bodies (EBs) or outer membrane complexes (COMCs), with lipopolysaccharide (LPS) from a Chlamydia organism, wherein the Chlamydia organism is preferably C. psittaci or C. trachomatis, more preferably C. psittaci of the Baker strain. The effect of the vaccine may be enhanced by addition of an adjuvant.

The MOMP in the vaccine is provided in purified form or in the context of *n EBs or COMCs. MOMP can be purified by various methods including but not limited to chromatographically or electrophoretically.

l Electrophoretic purification of MOMP (MOMP-E) is accomplished in the following manner. Chlamydia harvest fluid is centrifuged, and the resulting pellet is 25 resuspended in water. Samples are then resolved by gel electrophoresis, preferably sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins in the samples are solubilized, preferably by heating at about 95°C for approximately minutes in buffer, preferably Tris buffer, pH 6.8, containing SDS, 2-mercaptoethanol, glycerol, and bromophenol blue. Electrophoresis is carried out using polyacrylamide gels. The gels are stained, then briefly destained. The 40 kDa band is then excised, placed in a dialysis bag, and the protein is electroeluted out of the gel.

Chromatographic purification of MOMP (MOMP-C) is performed in the following manner. Non-MOMP proteins are extracted from EBs, preferably by z J lA e i-oh.

1 I WO 95/12411 PCT/US94/12626 treatment with N-lauroyl-sarcosine in phosphate buffered saline (PBS) containing EDTA for 1 hour at 37°C. Following extraction, the solution is centrifuged. The resulting pellet is washed and resuspended in buffer, preferably sodium phosphate buffer containing MgCl, deoxyribonuclease and ribonuclease A. The suspension is then incubated at about 37 0 C for approximately 2 hours and spun. The resulting pellet is washed and resuspended in buffer, preferably PBS containing sodium dodecyl sulfate and EDTA. The suspension is incubated again at about 37 0 C and then spun. The resulting supernatant is dialyzed against buffer, preferably sodium phosphate buffer, containing dithiothreitol and SDS, then loaded onto a hydroxylapatite column previously equilibrated with the same buffer. The column is washed and a linear gradient from about 0.1 to about 0.6 M sodium phosphate, pH 6.4, containing dithiothreitol and SDS is run. The pellet from the SDS extraction is re-extracted. Fractions from the supernatant and the pellet containing MOMP are pooled and then dialyzed against water.

The MOMP preparation in the vaccine can also be provided in the context of EBs or COMCs.

The EB subfraction preparation is isolated from Chlamydia harvest fluid.

Chlamydia organisms, preferably Chlamydia psittaci, more preferably Baker strain, is propagated in mammalian cells. The harvest fluid from the cells is concentrated, layered on top of 35% Renografin-76, and centrifuged. The pellet is resuspended, layered on top of a discontinuous gradient of diatrizoate meglunione and diatrizoate sodium and centrifuged. The band at the 44-52% interface which contains the EBs is collected, washed and resuspended in buffer, preferably a phosphate buffer, more preferably 0.01 M phosphate buffer, pH 8.0, containing 0.15 M NaCI (PBS). The EBs are then inactivated, preferably with binary ethylenimine (BEI), Ppropiolactone, formalin or glutaraldehyde. If BEI is used in inactivation the solution must be neutralized, preferably by addition of sodium thiosulfate.

To isolate the COMC preparation, the inactivated EBs are centrifuged and the resulting pellet is solubilized, preferably using PBS containing N-lauroylsarcosine and EDTA. The solution is then centrifuged, the resulting pellet being washed and resuspended in PBS.

Lipopolysaccharide (LPS) is also added to the vaccine. LPS is isolated via electrophoresis. Samples of the harvest fluid pellet are prepared for electrophoresis as described for the MOMP-E. Electrophoresis is carried out on polyacrylamide gels. It is preferred to use either a Tris/tricine buffer system or a Tris/glycine buffer system with interior resolution, The portion of the gel below the 6 kDa marker is cut off and placed in a basic solution, preferably 0.1 M glycine-NaOH, pH 11.0 and c i WO 95/12411 PCTIUS94/12626 incubated. The liquid is separated from the gel, the pH is adjusted to neutrality and then dialyzed.

The identity of the antigen is confirmed and the protein concentrations are determined. MOMP-C, MOMP-E, EBs, COMCs and LPS are placed in vials and lyophilized. The amount of LPS is determined by weighing the lyophilized samples. Vials are rehydrated with a pharmaceutically acceptable carrier. Such carriers include normal isotonic saline, standard 5% dextrose in water or water, preferably adjuvanted. Examples of adjuvants include, but are not limited to, Quil A, Alhydrogel, and Quil A and 5% Alhydrogel in tissue culture media. Vials containing LPS are rehydrated first. These solutions are then used to rehydrate the MOMP preparations resulting in a vaccine containing both the MOMP preparation and the LPS preparation.

The vaccine is administered to an animal suffering from a Chlamydial infection. The vaccine is also administered to healthy animals as immunization against infection by a Chlamydia organism. The vaccine can be administered subcutaneously, intramuscularly, intraperitoneally, intravitreally, orally, intranasally or by suppository at doses ranging from 0.01-100 pg/dose of MOMP and LPS each.

As used within the specification, a "MOMP preparation" refers to any vaccine preparation having purified MOMP, including, but not limited to, MOMP-E and MOMP-C, and MOMP in the context of EBs or COMCs. "LPS preparation" refers to a vaccine preparation having purified lipopolysaccharide. "Effective amount" refers to that amount of vaccine which invokes in an animal infected by a Chlamydia organism an immune response sufficient to kill the organism.

"Adjuvant" refers to materials which when injected on their own produce a state of nonspecific immunity expressed as a heightened resistance to infection. An example is Quil A in 5% Alhydrogel in tissue culture media.

This invention is further illustrated by the following nonlimiting examples.

Example 1: Subfraction Antigen Preparations Chlamydia harvest fluid. Chlamydia psittaci, Baker strain was propagated in dog kidney (DK) cells in DMEM with 2% fetal bovine serum. The harvest fluid was inactivated with 1% BEI and the solution neutralized by addition of 0.25% sodium thiosulfate.

Chlamydia elementary bodies (EBs). Non-inactivated Chlamydia harvest fluid was concentrated using a stirred cell concentrator or by centrifugation. The concentrate or pellet was layered on top of 35% Renografin-76 (Squibb Diagnostics, New Brunswick, NJ 08903), and centrifuged at 43,000 g for 1 hour. The pellet was WO 95/12411 PCT/US94/12626 resuspended and layered on top of a discontinuous gradient of 40, 44 and 52% Renografin-76 (Squibb Diagnostics, New Brunswick, NJ 08903) and centrifuged at 43,000 g for 1 hour, The band at the 44-52% interface containing the EBs was collected, washed and resuspended in 0.01 M phosphate buffer, pH 8.0, containing 0.15 M NaCI (PBS). The EBs were inactivated with 1% BE1 and the solution neutralized by addition of 0.25% sodium thiosulfate.

Chlamydia outer membrane complexes (COMCs). Inactivated EBs were centrifuged at 100,000 g for 1 hour at 10 0 C, and the resulting pellets solubilized with PBS containing 2% N-lauroyl-sarcosine and 1.5 mM EDTA for 1 hour, at 37 0 C. The solution was subjected to centrifugation at 100,000 g for 1 hour and the pellet washed once in PBS and then resuspended in PBS.

Example 2: Subunit Antigen Preparation Chromatographically purified MOMP (MOMP-C). The MOMP was chromatographically purified using a modification of the method described by Caldwell et al. (1981) Infect. Immun., 31:1161-1176. Briefly, EBs prepared as above were treated with 2% N-lauroyl-sarcosine in PBS containing 1.5 mM EDTA for 1 hour at 37 0 C to extract non-MOMP proteins from the outer membrane complex. The solution was centrifuged at 100,000 g for 1 hour, and the resulting pellet was washed in PBS then resuspended in 3-5 ml of 0.02 M sodium phosphate containing 10 mM MgC12 and 25 pg each of deoxyribonuclease I and ribonuclease A. This suspension was incubated at 37 0 C for 2 hours then centrifuged at 100,000 g for 1 hour. The pellet was washed in PBS, then resuspended in 2% sodium dodecyl sulfate (SDS) in PBS with 1.5 mM EDTA and incubated for 1 hour at 37 0 C. The suspension was centrifuged at 100,000 g for 1 hour. The resulting supernatant was dialyzed again 0.01 M sodium phosphate, pH 6.4, containing 1 mM dithiotreitol and 0.1% SDS (start buffer) and loaded onto a hydroxylapatite column which had been equilibrated in start buffer. The column was washed with start buffer, and then a 150 ml linear gradient of 0.1 to 0.6 M sodium phosphate, pH 6.4, containing 1 mM dithiotreitol and 0.1% SDS was initiated and 1 ml fractions were collected. The pellet from the SDS extraction was re-extracted for 30 minutes each at 37 0 C with periodic sonication sequentially using each of the following buffers: 1% N-lauroylsarcosine in 0.01 M sodium phosphate, pH 7.4; 1% N-lauroyl-sarcosine and 10 mM dithiotreitol in 0.01 M sodium phosphate, pH 7.4; and 1% octylglucoside and mM dithiotreitol in 0.01 M sodium phosphate, pH 7.4 according to the method of Bavoil et al. (1984) Infect. Immuno., 44:479-485. Fractions from the supernatant and the pellet containing MOMP were finally pooled and dialyzed against water.

-6- WO 95/12411 PCT/US94/12626 Electrophoretically purified MOMP (MOMP-E). Chlamydia harvest fluid was centrifuged at 25,000 g and the resulting pellets were resuspended in distilled water. Samples were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Proteins were solubilized by heating at 950C for minutes in a buffer containing 2% SDS, 5% 2-mercaptoethanol 3% glycerol, 0.002% bromophenol blue, and 50 mM Tris (pH 6.8).

Electrophoresis was carried out using 10% polyacrylamide gels (1.5 mm thick) in the discontinuous buffer system of Laemmli (1970) Nature (London), 227:680-685. The gels were stained with Coomassie Blue G in 50% methanol and 10% acetic acid, and briefly destained in 50% methanol and 10% acetic acid. The kDa band was then excised, placed in a 12-14 kDa molecular weight cut-off dialysis bag and the protein electroeluted out of the gel for 1 hour at 50 V.

Lipopolysaccharide (LPS). Samples of the harvest fluid pellet were prepared for electrophoresis as described for the MOMP-E. Electrophoresis was carried out on 12.5% polyacrylamide gels using the Tris/tricine buffer system of Schagger and von Jagow (1987) Anal. Biochem. 166:368-379, wherein the cathode buffer was 0.1 M Tris, 0.1M Tricine, 0.1% SDS, pH 8.25 and the anode buffer was 0.2M Tris, pH 8.9. The portion of the gel below the 6 kDa marker was cut off and placed in a solution of 0.1 M glycine-NaOH, pH 11.0 and incubated at 37 0 C for 3 hours. The liquid was then removed from the gel, the pH adjusted to neutrality and then dialyzed against distilled water using 1 kDa molecular weight cut-off dialysis membrane.

Example 3: Quantitation and Evaluation of Protein and LPS Protein was quantitated using a BCA assay kit (Pierce, P.O. Box 1A, Rockford, Illinois 61105). The amount of LPS was determined by weighing lyophilized samples. Prior to vaccine preparation, antigens were evaluated for purity by staining SDS-polyacrylamide gels with Coomassie Blue G in methanol and 10% acetic acid, and briefly destained in 50% methanol and acetic acid. The identity of the antigens was confirmed by western blot analysis using polyclonal antiserum specific for C. psittaci generated in cats. The p"cedure involved electrophoretically transferring f:actioned antigens from SDS gels onto Immobilon® membranes (Millipore Corporation, Bedford, MA 01730). The membranes were then blocked with 5% instant non-fat dry milk in PBS followed by incubation with the specific antibody for 1 hour at room temperature. Blots were then washed in PBS containing 0.3% Tween and incubated with goat anti-cat alkaline phosphatase-labeled antibody (Kirkegaard Perry Laboratories Inc., 2 WO 95/12411 PCT/US94/12626 Cessna Court, Gaithesburg, Maryland 20879). After extensive washing of the membranes, color was developed using BCIP/NBT substrate (Kirkegaard Perry Laboratories Inc., 2 Cessna Court, Gaithesburg, Maryland 20879). Stained gels and blots were analyzed by optical image scanning using the Bio Image System (Millipore Corporation, Bedford, MA 01730).

Example 4: Vaccine Preparation Subfraction antigen preparation for studies I-III were formulated as follows for vaccination of mice. For study I, a specific lot of unprocessed infected tissue culture fluid was used as a positive control since previous studies demonstrated its efficacy. To prepare this vaccine lyophilized fluid was rehydrated with 200 pg/ml Quil A in 5% Alhydrogel in RPMI at a dilution that had been shown to protect mice in past experiments. The total protein present in this dose was 8.1 pg/100 pl. The quantity of EBs in the vaccine was determined by standardizing to the western blot intensity of the MOMP band in the harvest fluid. Based on this, the protein concentration in the EB vaccine was 0.575 pg/100 pl. Three 10-fold serial dilutions of both harvest fluid and EB vaccines were also made.

For study II, EBs and COMCs were formulated as follows. The quantity of COMCs in the vaccine was determined by standardizing to the western blot intensity of the MOMP band in a purified EB preparation. The purified EB preparation was then diluted in PBS to equal the MOMP concentration in COMCs. These were then further diluted in adjuvant (2.5 pg/ml Quil A) in 0.85% NaCl to a total protein concentration of 5 pg/100 pl. The same procedure was followed to produce the COMC vaccine resulting in a protein concentration of 2.8 ug/100 pi and 0.28 pg/100 pl.

To formulate subunit antigens predetermined quantities of each antigen were aliquoted into vials and lyophilized. Vials containing the MOMP-C, MOMP-E and LPS were rehydrated with 200 pg/ml Quil A in 5% Alhydro;el in RPMI medium to yield a concentration of 100 pg/100 pl. Rehydrated LPS was used to rehydrate a few aliquots of the MOMP-E to provide a MOMP-E+LPS vaccine containing 100 pg of each antigen in a 100 pl total volume. Three 10-fold dilutions of the above 4 vaccines were made in 200 pg/ml Quil A in 5% Alhydrogel diluted in RPMI. All vaccines were stored at 4 0 C between, he time of preparation and administration (1 day for the first dose and 14 days for the second dose).

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WO 95/12411 PCTIUS94/12626 Example 5: Vaccination and Challenge Female Swiss Whi;e CF-1 mice weighing 12-14 grams (Charles River) received 2 vaccinations of 100 pl subcutaneously, two weeks apart. There were 8 mice in each group with the exception of the 10 pg group for the MOMP-C, MOMP-E, LPS, MOMP-E+LPS groups, the 0.058 pg group for the EB and the 0.81 aeg group for the harvest fluid in which there were 10 mice per group. The 2 additional mice in these groups were sacrificed and bled on the day the remainder of the mice were challenged. Vaccinates as well as 10 controls were challenged with an intraperitoneal inoculation of C. psittaci, Cello strain (Cello (1967) Am. J.

Ophthalmol., 63:1270-1273) 2 weeks after the second immunization.

For each study, 3 additional groups of 10 mice were challenged with serial dilutions of the challenge material to confirm the LD 50 At the dilution of the Chlamydia organisms used to challenge vaccinates and at a 1:10 dilution of this challenge material all the control mice (10 per group) died.

All subunit preparations, except LPS, protected 100% of the mice when administered at 100 pg/dose. A dose-related titration of the protective effect was also observed. Comparison of the various MOMP-containing preparations administered at the 10 pg dose indicated that both MOMP-C alone and MOMP-E plus LPS still protected 100% of the mice, while MOMP-E could only induce a protective level. Comparable levels of protection were also observed with the pg and 0.1 pg doses of the MOMP-C alone and MOMP-E plus LPS preparations.

These levels were significantly greater than that induced by MOMP-E alone. See data provided in Table 1.

,i WO 95/12411 PCT/US94/12626 TABLE 1 Protection of Mice with Chlamydia Subunit Preparations Subunit Preparation Total Protein ProtectionLevel Antigen/Adjuvant (ug) survivors/total MOMP-C 100 8/8(100) 2% ALOH, 25 pg Quil A 10 8/8 (100) 6/8 0.1 0/8 (0) MOMP-E 100 8/8 (100) 2% ALOH, 25 pg Quil A 10 5/8 (62.5) 3/8 (37/5) 0.1 0/8 (0) LPS 100 3/8 (37/5) 2% ALOH, 25 pg Quil A 10 3/8 (37/5) 0/8 (0) 0.1 2/8 MOMP-E LPS 100 8/8 (100) 2% ALOH, 25 pg Quil A 10 8/8 (100) 5/8 (63.5) 0.1 1/8 (12.5) Example 6: Evaluation of Immune Response by ELISA EBs were fixed with methanol to 96-well round-bottom Immulonb 2 plates (Dynatech Laboratories, 14340 Sullyfield Circle, Chantilly, Virginia) at 1 pg/well.

Plates were washed with distilled water, then blocked with 3% horse serum (Hy- Clone Laboratories, Inc., 1725 South HyClone Road, Logan, Utah 84321) in PBS for 1 hour at room temperature. Mouse sera was diluted in PBS containing 0.3% Tween and applied to plates. After a 1 hour incubation at room temperature, plates were washed with PBS containing 0.3% Tween and further incubated with peroxidase-labeled antiserum to mouse IgG (Kirkegaard Peny Laboratories Inc., 2 Cessna Court, Gaithesburg, Maryland 20879). Plates were then washed with PBS containg 0.3% Tween and developed with ABTS substrate (Kirkegaard Perry Laboratories Inc., 2 Cessna Court, Gaithesburg, Maryland 20879). After minutes, the O.D. 405-490 was measured. Titers are expressed as the dilution of r WO 95/12411 PCT/US94/12626 mouse serum giving an O.D. reading of 0.200. Background values were consistently between 0.002-0.004 O.D. units.

Serum titers were determined for two randomly chosen mice from each dose group prior to being challenged. A correlation between the ability of the vaccines to protect mice from infection and the EB-specific serum response was observed.

Western blot analysis from mice vaccinated with MOMP-C alone and MOMP-E plus LPS indicated that the response was almost exclusively directed to the MOMP.

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Claims (31)

1. A vaccine for immunizing or treating an animal against infection by strains of Chlamydia psittaci to which said animal is susceptible, comprising: a preparation of major outer membrane protein, MOMP, from one or more of said strains of Chlamydia psittaci wherein said MOMP comprises one or more members selected from the group consisting essentially of: MOMP of molecular weight about 40 kDa which has been substantially purified by separation from whole cell lysates of one or more of said strains of Chlamydia psittaci; MOMP of molecular weight about 40 kDa designated MOMP-E, which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE; MOMP of molecular weight about 40 kDa designated MOMP-C, which has been prepared by: treating whole cell lysates of EBs of said strains of Chlamydia psittaci with meglumine acetrizoate or diatrizoate, or sodium diatrizoate, or mixtures thereof and centrifugation to provide a purified EB subfraction; inactivating said EB subfraction; 20 solubilizing and theieby removing non-MOMP proteins from said EB subfraction by treatment with N-lauroyl-sarcosine and centrifugation whereby a sarcosyl- ""'insoluble fraction remains consisting essentially of empty EBs with outer membranes, designated chlamydia outer membrane complexes, COMCs; extracting said MOMP-C from said COMCs fraction by treating said 25 fraction with sodium dodecyl sulfate, SDS; and separating said MOMP-C from said SDS treated COMCs fraction and substantially purifying the same by means of chromatography; a preparation of lipopolysaccharide, LPS, from one or more of said strains of Chlamydia psittaci which has been separated from whole cell lysates of elementary 0 bodies, EBs, of said strains of Chlamydia psittaci by means of polyacrylamide gel electrophoresis wherein a portion of said gel below about 6 kDa is removed and incubated and the resulting solution is purified to give said LPS; a pharmaceutically acceptable carrier; R i~i~p I, 13 and optionally, an adjuvant.

2. A vaccine according to claim 1, wherein said Chlamydiapsittaci strain is ATCC VR-120.

3. A vaccine according to claim 1, wherein said MOMP comprises MOMP-E, which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE.

4. A vaccine according to claim 1, wherein said MOMP comprises MOMP-C.

5. A vaccine according to claim 4, wherein meglumine diatrizoate and sodium diatrizoate are used to treat whole cell lysates of EBs in order to provide said purified EB subfraction. p p p p op.

6. A vaccine according to claim 4, wherein said EB subfraction is inactivated by treatment with binary ethylenimine, BEI; P-propiolactone; formalin; or glutaraldehyde.

7. A vaccine according to claim 4, wherein said chromatography used to separate said MOMP-C from said SDS treated COMCs fraction and substantially purify the same is carried out by means of a chromatographic hydroxylapatite column utilizing a sequence of 0.1% SDS and 1% N-lauroyl-sarcosine buffers.

8. A vaccine according to any one of claims 1-7, containing an adjuvant 20 comprising one or more members selected from the group consisting essentially of aluminum hydroxide and Quil A.

9. A vaccine according to any one of claims 1-8, wherein said preparation of major outer membrane protein, MOMP, is electrophoretically homogeneous.

10. A method for treating Chlamydia psittaci infections in an animal 25 susceptible thereto comprising administering to said animal an effective amount of a vaccine comprising: a preparation of major outer membrane protein, MOMP, from one or more of said strains of Chlamydia psittaci wherein said MOMP comprises one or more members selected from the group consisting essentially of: MOMP of molecular weight about 40 kDa which has been substantially purified by separation from whole cell lysates of one or more of said strains f Chlamydiapsittaci; S(2) MOMP of molecular weight about 40 kDa designated MOMP-E, i"Y waasa~a~--~. I a a 6606 *6 4 66 6 0a a 4~ 4 46 oe%. p* 04 *s 6 604*694 66o 46Oh 4g 4e 4 *4%I* 46 4a ca 14 which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE; MOMP of molecular weight about 40 kDa designated MOMP-C, which has been prepared by: treating whole cell lysates of EBs of said strains of Chlamydia psittaci with meglumine acetrizoate or diatrizoate, or sodium diatrizoate, or mixtures thereof and centrifugation to provide a purified EB subfraction; inactivating said EB subfraction; 10 solubilizing and thereby removing non-MOMP proteins from said EB subfraction by treatment with N-lauroyl-sarcosine and centrifugation whereby a sarcosyl-insoluble fraction remains consisting essentially of empty EBs with outer membranes, designated chlamydial outer membrane complexes, COMCs; extracting said MOMP-C from said COMCs fraction by treating said fraction with sodium dodecyl sulfate, SDS; and separating said MOMP-C from said SDS treated COMCs fraction and substantially purifying the same by means of chromatography; a preparation of lipopolysaccharide, LPS, from one or more of said strains of Chlamydia psittaci which has been separated from whole cell lysates of elementary 20 bodies, EBs, of said strains of Chlamydia psittaci by means of polyacrylamide gel electrophoresis wherein a portion of said gel below about 6 kDa is removed and incubated and the resulting solution is purified to give said LPS; a pharmaceutically acceptable carrier; and optionally, 25 an adjuvant.

11. A method according to claim 10, wherein said Chlamydia psittaci strain is ATCC VR-120.

12. A method according to claim 10, wherein said MOMP comprises MOMP- E, which has been separated from whole cell lysates of elementary bodies, EBs, of said 30 strains of Chlamydia psittaci by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE.

13. A method according to claim 10, wherein said MOMP comprises MOMP- 6 4 40 45 4 4 0 2 9 4 p r^r .L r I^

14. A method according to claim 13, wherein meglumine diatrizoate and sodium diatrizoate are used to treat whole cell lysates of EBs in order to provide said purified EB subfraction.

A method according to claim 14, wherein said EB subfraction is inactivated by treatment with binary ethylenimine, BEI; P-propiolactone; formalin; or glutaraldehyde.

16. A method according to any one of claims 10-15, containing an adjuvant comprising one or members selected from the group insisting essentially of aluminum hydroxide and Quil A.

17. A method according to any one of claims 10-16, wherein said preparation of major outer membrane protein, MOMP, is electrophoretically homogeneous.

18. A method for immunizing and treating an animal against infection by strains of Chlamydia psittaci to which said animal is susceptible, comprising: administering to a healthy said animal an immunogenically and therapeutically effective amount of a vaccine comprising: Jii a a, a a ar *t a a aa4r, a a a a. a *r 4 4ga a. ar g a r a a 'a e a. a o a, a.*r aa a a a a a preparation of major outer membrane protein, MOMP, from one or more of said strains of Chlamydia psittaci wherein said MOMP comprises one or more members selected from the group consisting essentially of: MOMP of molecular weight about 40 kDa which has been substantially 5 purified by separation from whole cell lysates of one or more of said strains of Chlamydia psittaci; MOMP of molecular weight about 40 kDa designated MOMP-E, which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE; MOMP of molecular weight about 40 kDa designated MOMP-C, which has been prepared by: treating whole cell lysates of EBs of said strains of Chlamydia psittaci with meglumine acetrizoate or diatrizoate, or sodium diatrizoate, or mixtures thereof and is centrifugation to provide a purified EB subfraction; inactivating said EB subfraction; solubilizing and thereby removing non-MOMP proteins from said EB subfraction by treatment with N-lauroyl-sarcosine and centrifugation whereby a sarcosyl-insoluble fraction remains consisting essentially of empty EBs with outer 20 membranes, designated chlamydial outer membrane complexes, COMCs; extracting said MOMP-C from said COMCs fraction by treating said fraction with sodium dodecyl sulfate, SDS; and separating said MOMP-C from said SDS treated COMCs fraction and -bstantially purifying the same by means of chromatography; 25 a preparation of lipopolysaccharide, LPS, from one or more of said strains of Chlamydia psittaci which has been separated from whole cell lysates of elementary bodies, EBs, of said strains of Chlamydia psittaci by means of polyacrylamide gel electrophoresis wherein a portion of said gel below about 6 kDa is removed and incubated and the resulting solution is purified to give said LPS; 30 a pharmaceutically acceptable carrier; and optionally, an adjuvant.

19. A method according to claim 18, wherein said Chlaimydia psittaci strain is ATCC VR-120. 35

20. A method according to claim 18, wherein said MOMP comprises MOMP- E, which has been separated from whole cell lysates of elementary bodies, EB, of said strains of Chlamydia psittaci by means of sodium dodscyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE.

21. A method according to claim 18, whermn said MOMP comprises MOMP- a aa a a a egagag 40 *c a a a a ac a C.

22. A method according to claim 21, wherein meglumine diatrizoate and sodium diatrizoate are used to treat whole cell lysates of EBs in order to provide said purified EB subfraction.

23. A method according to claim 21, wherein said EB subfraction is inactivated by treatment with binary ethylenimine, BEI; 3-propiolactone; formalin; or glutaraldehyde. i4

24. A method according to claim 21, wherein said chromatography used to separate said MOMP-C from said SDS treated COMCs fraction and substantially purify the same is carried out by means of a chromatographic hydroxylapatite column utilizing a sequence of 0.1% SDS and 1% N-lauroyl-sarcosine buffers. 5

25. A method according to any one of claims 18-24, containing an adjuvant comprising one or more members selected from the group consisting essentially of aluminium hydroxide and Quil A.

:26. A method according to any one of claims 18-25, wherein said preparation of major outer membrane protein, MOMP, is electrophoretically homogeneous. 10

27. A vaccine for immunizing or treating an animal against infection by strains of Chlamydiapsittaci substantially as hereinbefore specifically described.

28. A vaccine according to any of claims 1 to 9 when used for the treatment of Chlamydiapsittaci infections in an animal susceptible thereto.

29. The use of a vaccine according to any of claims 1 to 9 for the preparation of a 15 medicament for treating Chlamydiapsittaci infections in an animal susceptible thereto.

•30. A vaccine according to any of claims 1 to 9 when used for immunizing an animal against infection by strains of Chlamydia psittaci to which said animal is pI susceptible.

31. The use of a vaccine according to any of claims 1 to 9 for the preparation of a S 20 medicament for immunizing an animal against Chlamydia psittaci to which said animal is susceptible. Dated 27 November, 1998 Pfizer Inc. a, Patent Attorneys for the Applicant/Nominated Person S* 25 SPRUSON FERGUSON a a0 0a a a. a a a a o a iV d J :u J *I 6 P r\G 4 k^ T\ A/