AU706021B2 - Piperazine derivatives - Google Patents

Description

Q:\OPERW\5V1031-97SPE 12/3/99 -1- PIPERAZINE DERIVATIVES TECHNICAL FIELD The present invention relates to new piperazine derivatives and a pharmaceutically acceptable salt thereof.

More particularly, it relates to new piperazine derivatives and a pharmaceutically acceptable salt thereof which have pharmacological activities such as Tachykinin antagonism, especially Substance P antagonism, Neurokinin A antagonism, Neurokinin B antagonism, and the like, to a process for preparation thereof, to a pharmaceutical composition comprising the same, and to a use of the same as a medicament.

Accordingly, the present invention advantageously provides new and useful piperazine derivatives and a pharmaceutically acceptable salt thereof which have pharmacological activities such as Tachykinin antagonism, especially Substance P antagonism, Neurokinin A antagonism, Neurokinin B antagonism, and the like.

*00 0 0 a 0 0 00 .0 QOPER\MJC'57031-97.SPE 12/3;99 -2- DISCLOSURE OF INVENTION The present invention provides a compound of the following formula

(I),I

F

3

C'

000 0 0 20 namely (2R)-l-[3,5-bis (trifluoromethyl)benzoyl3-2- (lH-indol- 3-ylmethyl) (4-methyl-1-piperazinyl) carbamoylmethyl] piperazine, or fumaric acid salt thereof, namely bis (trifluoromethyl)benzoyll-2- (lH-indol-3-ylmethyl) (4methyl-l-piperazinyl) carbamoylmethyllpiperazine fuinarate [hereinafter referred to briefly as compound (If)]3.

The present invention also provides compounds represented by the following general formula (Ig): .4 5* S 6

S.

OS OS SC S 0

S

-3 (I g) wherein Ris trihalo(lower)alkyl, Ris trihalo(lower)alkyl,

R

3 is indolyl(lower)alkyl, 0 **ago: is -CH 2 or C2 9 6 4e N Na.- R is 0-, R 50Q ,and N

R

6 R~ 7 or N

R

-f7 ,00 0 0 *0 00 0 0* 0.

9 0 00 00 9000 0 9 000.0.

0 9 in which RS is hydrogen or lower alkoxycarbonyl, Ris hydrogen or lower alkanoyl,

R

7 is hydrogen, lower alkyl, lower alkanoyl, lower alkoxycarbonyl, lower alkoxy(lower) alkanoyl, cyclo(lower)alkylcarbonyl, aroyl or lower alkylsulfonyl, or its pharmaceutically acceptable salt.

According to the present invention, the compounds can be prepared by processes which are illustrated in the .ollowing schemes.

WO 96/37489 Process 1 PCT/JP96/01335

H

N' C OH

H

2 N-N

NCH

3

(III)

or its reactive derivative at the amino group.

or a salt thereof

(ITI)

or its reactive derivative at the carboxy group or a salt thereof

N

H

MI

or a salt thereof Process 2 H0 2

CCH

CH

3 H9 2

(I)

or a salt thereof other than fumaric acid salt thereof WO 96/37489 PCT/JP96/01335 5

HO

2

CCH

HCCO

2

H

(If) W-Al-R 4

(V)

or a salt thereof

(IV)

or its reactive derivative at the imino group or a salt thereof (Ig') or a salt thereof HOOC-Al-R 4

(VI)

or its reactive derivative at the carboxy group or a salt thereof 0 11 4

I-C-AI-R

(IV)

or its reactive derivative at the imino group or a salt thereof (Ig") or a salt thereof Q OPER, MJC-57031 97 SPE 12~ 3 99 -6wherein R 1

R

2

R

3 and R 4 are each as defined above; is -CH 2 and W is a leaving group.

Accordingly the present invention provides a process for preparing a compound of the formula

H

O*#S

*so* .9.

or fumaric acid salt thereof, which comprises reacting a compound of the formula: 2 :**25 5* 5> 0 C N C-OH

O

F

3

C

5* 0 or its reactive derivative at the carboxy group or a salt thereof with a compound of the formula: -6A 11N- \-/NCH 3 or its reactive derivative at the amino group or a salt thereof to give a compound of the formula

H

N

S

*e S

S

OS..

S

9SS.

*0

S

or fuinaric acid salt thereof, or reacting a compound of the formula

S..

9 .9 9

CF

3 430 0:000: or a salt thereof other than fumaric acid salt thereof with fumaric acid to give a compound of the formula: Q %0PER\NIJC\57031-97 SPE 1239W 6B

H

CF3 .H 2CC

H

HO

2

CCH

0 NCH 3

HCCO

2

H

F

3 C CN _N ,C N

S

H

0 Suitable salts and pharmaceutically acceptable salts of the starting and object compounds are conventional non-toxic salt and include an acid addition salt such as an organic acid salt acetate, trifluoroacetate, fumarate, maleate, tartrate, methanesulfonate, benzenesulfonate, formate, toluenesulfonate, etc.), an inorganic acid salt (e.g.

hydrochloride, hydrobromide, hydroiodide, sulfate, nitrate, phosphate, etc.), or a salt with an amino acid (e.g.

arginine, aspartic acid, glutamic acid, etc.), or a metal salt such as an alkali metal salt sodium salt, potassium salt, etc.) and an alkaline earth metal salt (e.g.

calcium salt, magnesium salt, etc.), an ammonium salt, an organic base salt trimethylamine salt, triethylamine salt, pyridine salt, picoline salt, dicyclohexylamine salt, N,N'-dibenzylethylenediamine salt, etc.), or the like.

0 o S o*

C

-6C- In the above and subsequent descriptions of the present specification, suitable examples and illustrations of the various definitions which the present invention include within the scope thereof are explained in detail as follows.

The term "lower" as used in the claims and the description is intended to mean 1 to 6, preferably 1 to 4 carbon atom(s), unless otherwise indicated.

Suitable "lower alkyl" and "lower alkyl moiety" in the terms "indolyl(lower)alkyl" and "lower alkylsulfonyl" is straight or branched one having 1 to 6 carbon atom(s) and may include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, pentyl, hexyl and the like.

Suitable "trihalo(lower)alkyl" may include trichloromethyl, tribromomethyl, trifluoromethyl and the like.

Suitable "lower alkoxy" and "lower alkoxy moiety" in the 0 00 S o 7 terms "lower alkoxycarbonyl" and "lower alkoxy(lower)alkanoyl" may include methoxy, ethoxy, isopropyloxy, butoxy and the like.

Suitable "lower alkanoyl" and "lower alkanoyl moiety" in the term "lower alkoxy(lower)alkanoyl" may include formyl, acetyl, propionyl, butyryl, isobutyryl, valeryl, hexanoyl, pivaloyl and the like.

Suitable "cyclo(lower)alkyl moiety" in the term "cyclo(lower)alkylcarbonyl" may include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and the like.

Suitable "aroyl" may include benzoyl, toluoyl, naphthoyl and the like.

Suitable "leaving group" may include hydroxy, reactive group derived from hydroxy and the like.

Suitable "reactive group derived from hydroxy" may include acid residue and the like.

qSuitable "acid residue" may include halogen (e.g.

fluoro, chloro, bromo, iodo), acyloxy acetoxy, tosyloxy, mesyloxy, etc.) and the like.

0b The Processes 1 to 4 for preparing the compounds of the present invention are explained in detail in the following.

.5 Process 1 *e The compound or a salt thereof can be prepared by reacting a compound (II) or its reactive derivative at the carboxy group or a salt thereof with a compound (III) or its reactive derivative at the amino group or a salt thereof.

Suitable reactive derivative at the carboxy group of the compound (II) may include an acid halide, an acid anhydride, an activated amide, an activated ester, and the like.

WO 96/37489 PCT/JP96/01335 8 Suitable examples of the reactive derivatives may be an acid chloride; an acid azide; a mixed acid anhydride with an acid such as substituted phosphoric acid [e.g.

dialkylphosphoric acid, phenylphosphoric acid, diphenylphosphoric acid, dibenzylphosphoric acid, halogenated phosphoric acid, etc.], dialkylphosphorous acid, sulfurous acid, thiosulfuric acid, sulfuric acid, sulfonic acid [e.g.

methanesulfonic acid, etc.], aliphatic carboxylic acid [e.g.

acetic acid, propionic acid, butyric acid, isobutyric acid, pivalic acid, pentanoic acid, isopentanoic acid, 2-ethylbutyric acid, trichloroacetic acid, etc.] or aromatic carboxylic acid benzoic acid, etc.]; a symmetrical acid anhydride; an activated amide with imidazole, 4-substituted imidazole, dimethylpyrazole, triazole or tetrazole; or an activated ester cyanomethyl ester, methoxymethyl ester, dimethyliminomethyl

[(CH

3 2 N=CH-] ester, vinyl ester, propargyl ester, p-nitrophenyl ester, 2 ,4-dinitrophenyl ester, trichlorophenyl ester, pentachlorophenyl ester, mesylphenyl ester, phenylazophenyl ester, phenyl thioester, p-nitrophenyl thioester, p-cresyl thioester, carboxymethyl thioester, pyranyl ester, pyridyl ester, piperidyl ester, 8-quinolyl thioester, etc.], or an ester with a N-hydroxy compound N,N-dimethylhydroxylamine, 1-hydroxy-2-(1H)pyridone, N-hydroxysuccinimide, N-hydroxyphthalimide, 1-hydroxy-lH-benzotriazole, etc.], and the like. These reactive derivatives can optionally be selected from the above according to the kind of the compound (II) to be used.

Suitable reactive derivative at the amino group of the compound (III) may include Schiff's base type imino or its tautomeric enamine type isomer formed by the reaction of the compound (III) with a carbonyl compound such as aldehyde, ketone or the like; a silyl derivative formed by the reaction of the compound (III) with a silyl compound such as bis(trimethylsilyl)acetamide, mono(trimethylsilyl)acetamide, bis(trimethylsilyl)urea or the like; a derivative formed by WO 96/37489 PCT/JP96/01335 9 reaction of the compound (III) with phosphorus trichloride or phosgene and the like.

The reaction is usually carried out in a conventional solvent such as water, alcohol methanol, ethanol, etc.], acetone, 2-butanone, dioxane, acetonitrile, chloroform, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine or any other organic solvent which does not adversely influence the reaction, or the mixture thereof.

In this reaction, when the compound (II) is used in a free acid form or its salt form, the reaction is preferably carried out in the presence of a conventional condensing agent such as N,N'-dicyclohexylcarbodiimide; N-cyclohexyl-N'-morpholinoethylcarbodiimide; N-cyclohexyl-N'-(4-diethylaminocyclohexyl)carbodiimide; N,N'-diethylcarbodiimide; N,N'-diisopropylcarbodiimide; N-ethyl-N'-( 3 -dimethylaminopropyl)carbodiimide; pentamethyleneketene-N-cyclohexylimine; diphenylketene-N-cyclohexylimine; ethoxyacetylene; 1-alkoxy-l-chloroethylene; trialky! phosphite; ethyl polyphosphate; isopropyl polyphosphate; phosphorus oxychloride (phosphoryl chloride); phosphorus trichloride; diphenyl phosphorylazide; thionyl chloride; oxaly! chloride; lower alkyl haloformate ethyl chloroformate, isopropyl chloroformate, etc.]; triphenylphosphine; 2 -ethyl-7-hydroxybenzisoxazolium salt; hydroxide intramolecular salt; 1-(p-chlorobenzenesulfonyloxy)-6-chloro-lHbenzotriazole; 1-hydroxybenzotriazole; so-called Mukaiyama reagent such as 2 -chloro-l-methylpyridinium iodide; 1-( 3 -dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by itself or in combination with 1-hydroxybenzotriazole; so called Vilsmeier reagent prepared by the reaction of N,N-dimethylformamide with thionyl chloride, phosgene, trichloromethyl chloroformate, phosphorus oxychloride, etc.; 10 or the like, or the mixture thereof.

The reaction may also be carried out in the presence of an inorganic or organic base such as an alkali metal bicarbonate, tri(lower)alkylamine, pyridine, N-(lower)alkylmorpholine, N,N-di(lower)alkylbenzylamine, or the like.

The reaction temperature is not critical, and the reaction is usually carried out under cooling to warming.

Process 2 The compound (If) can be prepared by reacting the compound or a salt thereof other than fumaric acid salt thereof with fumaric acid.

The reaction is usually carried out in a conventional solvent such as water, alcohol methanol, ethanol, etc.], acetone, 2-butanone, dioxane, acetonitrile, chloroform, methylene chloride, ethylene chloride, tetrahydrofuran, ethyl acetate, N,N-dimethylformamide, pyridine or any other organic solvent which does not 06"" adversely influence the reaction, or the mixture thereof.

*a The reaction temperature is not critical, and the reaction is usually carried out under cooling to warming.

Process 3 The compound or a salt thereof can be prepared by reacting the compound (IV) or its reactive derivative at the imino group or a salt thereof with the compound or a salt thereof.

Suitable reactive derivative at the imino group of the compound (IV) may include Schiff's base type imino or its tautomeric enamine type isomer formed by the reaction of the compound (IV) with a carbonyl compound such as aldehyde, ketone or the like; a silyl derivative formed by the reaction of the compound (IV) with a silyl compound such as bis(trimethylsilyl)acetamide, mono(trimethylsilyl)acetamide, m 11 bis(trimethylsilyl)urea or the like; a derivative formed by reaction of the compound (IV) with phosphorus trichloride or phosgene and the like.

This reaction is usually carried out in a solvent such as alcohol methanol, ethanol, etc.], dichloromethane, benzene, N,N-dimethylformamide, tetrahydrofuran, diethyl ether or any other solvent which does not adversely affect the reaction.

The raction may be carried out in the presence of an inorganic or an organic base such as an alkali metal hydroxide sodium hydroxide, potassium hydroxide, etc.], an alkali metal carbonate sodium carbonate, potassium carbonate, etc.], an alkali metal bicarbonate sodium bicarbonate, potassium bicarbonate, etc.], alkali metal hydride sodium hydride, potassium hydride, etc.], tri(lower)alkylamine trimethylamine, triethylamine, 9 diisopropylethylamine, etc.], pyridine or its derivative 9 picoline, lutidine, 4-dimethylaminopyridine, etc.], or the like. In case that the base to be used in liquid, it can also be used as a solvent.

The reaction temperature is not critical, and the reaction can be carried out under cooling, at room temperature or under warming or heating.

Process 4 The compound or a salt thereof can be prepared by reacting a compound (IV) or its reactive derivative at the imino group or a salt thereof with a compound (VI) or its reactive derivative at the carboxy group or a salt thereof.

The reaction can be carried out in the manner disclosed in Preparation 10 or similar manners thereto.

The compounds of the present invention have pharmacological activities such as Tachykinin antagonism, especially Substance P antagonism, Neurokinin A antagonism or

'I

Neurokinin B antagonism, and therefore are useful for treating or preventing Tachykinin-mediated diseases, particularly Substance P-mediated diseases, for examole, respiratory diseases such as asthma, bronchitis chronic bronchitis, acute bronchitis and diffuse panbronchiolitis, etc.), rhinitis, cough, expectoration, and the like; ophthalmic diseases such as conjunctivitis, vernal conjunctivitis, and the like; cutaneous diseases such as contact dermatitis, atooic dermatitis, urticaria, and other eczematoid dermatitis, and the like; inflammatory diseases such as rheumatoid arthritis, osteoarthritis, and the like; pains or aches migraine, headache, cluster headache, toothache, cancerous pain, back pain, neuralgia, etc.); and the like.

Further, it is expected that the compounds of the present invention are useful for treating or preventing ophthalmic diseases such as glaucoma, uveitis, and the like; gastrointestinal diseases such as ulcer, ulcerative colitis, irritable bowel syndrome, food allergy, and the like; inflammatory diseases such as nephritis, and the like, circulatory diseases such as hypertension, angina pectoris, cardiac failure, thrombosis, Raynaud's disease, and the like; epilepsy; spastic paralysis; pollakiuria; cystitis; bladder detrusor hyperreflexia; urinary incontinence; Parkinson diseases; dementia; AIDS related dementia; Alzheimer's diseases; Down's syndrome; Huntington's chorea; carcinoid syndrome; disorders related to immune enhancement or suppression; disorders caused by Helicobacter pylori or another spiral urease-positive gram-negative bacterium; sunburn; angiogenesis or diseases caused by angiogenesis; and the like.

It is furthermore expected that the compounds of :he present invention are useful for treating or preventing hronic obstructive pulmonary diseases, particularly chronic pulmonary emphysema; irvis; proiiferative vitreoretinopathy; psoriasis; inflammatory intestinal diseases, particularly Crohn's diseases; hepatitis; superficial pain on congelation, burn, herpes zoster or diabetic neuropathy; tenalgia attended to hyperlipidemia; postoperative neuroma, particularly of mastectomy; vulvar vestibulitis; hemodialysis-associated itching; lichen planus; laryngopharyngitis; bronchiectasis; coniosis; whooping cough; pulmonary tuberculosis; cystic fibrosis; emesis; mental diseases, particularly anxiety, depression, dysthymic disorders and schizophrenia; demyelinating diseases such as multiple sclerosis and amyotrophic lateral sclerosis; attenuation of morphine withdrawal; oedema, such as oedema caused by thermal injury; small cell carcinomas, particularly small cell lung cancer (SCLC); hypersensitivity disorders such as poison ivy; fibrosing and collagen diseases such as scleroderma and eosinophilic fascioliasis; reflex sympathetic dystrophy such as shoulder/hand syndrome; addiction disorders such as 00 alcoholism; stress related somatic disorders; rheumatic diseases such as fibrositis; and the like.

I a.

For therapeutic purpose, the compounds of the present invention can be used in a form of pharmaceutical preparation containing one of said compound, as an active ingredient, in admixture with a pharmaceutically acceptable 0* 0 5 carrier such as an organic or inorganic solid or liquid 6 excipient suitable for oral, parenteral, external including topical, enteral, intravenous, intramuscular, inhalant, nasal, intraarticular, intraspinal, transtracheal or transocular administration. The pharmaceutical preparations *So* may be solid, semi-solid or solutions such as capsules, tablets, pellets, dragees, powders, granules, suppositories, ointments, creams, lotions, inhalants, injections, cataplasms, gels, tapes, eye drops, solution, syrups, aerosols, suspension, emulsion, or the like. If desired, ,y 1here may be included in these preparations, auxiliary WO 96/37489 PCT/JP96/01335 14 substances, stabilizing agents, wetting or emulsifying agents, buffers and other commonly used additives.

While the dosage of the object compounds will vary depending upon the age and condition of a patient, an average single dose of about 0.1 mg, 1 mg, 10 mg, 50 mg, 100 mg, 250 mg, 500 mg and 1000 mg of the object compounds may be effective for treating Tachykinin-mediated diseases such as asthma and the like. In general, amounts between 0.1 mg/body and about 1,000 mg/body may be administered per day.

In order to illustrate the usefulness of the object compounds, the pharmacological test data of a representative compound of the present invention is shown in the following.

The following Test Compound showed more than inhibition rate of 125I-BH-Substance P binding to h-NK 1 receptors at the concentration of 0.1 pg/ml.

Test Compound The object compound of the Example 2 125I-BH-Substance P Binding to h-NK 1 Receptors Test Method 12 5I-BH-Substance P Binding to h-NK 1 Receptors Crude CHO cell membrane preparation CHO cells permanently expressing h-NK 1 receptors were harvested and homogenized with a Dounce homogenizer at 4 0 C in a buffer (0.25 M sucrose, 25 mM Tris-HC1 pH 7.4, 10 mM MgCl 2 1 mM EDTA, 5 pig/ml p-APMSF). The homogenate was centrifuged (500 x g, 10 minutes), and the pellet was resuspended in the same buffer, homogenized, and centrifuged. The two supernatants were combined and centrifuged (100,000 x g, 1 hour). The crude cell membranes thus isolated were resuspended in buffer (25 mM Tris-HCl pH 7.4, 10 mM MgCI 2 1 5 1 mM EDTA, 5 ag/ml p-APMSF) and stored at -80'C until use.

125I-BH-Substance P binding to preparation membrane Cell membranes (6 pg/ml) were incubated with 1 25

I-BH-

Substance P (0.1 nM) with or without test compounds in 0.25 ml of Medium 2 (50 mM Tris-HCl pH 7.4, 5 mM MnC1 2 20 jg/ml chymostatin, 40 pg/ml bacitracin, 4 ug/ml leupeptin, 5 pg/ml p-APMSF, 200 pg/ml BSA) at 22 0 C for 90 minutes. At the end of the incubation period, the content was quickly filtered over a Wahtman GF/C glass filter (pretreated with 0.1% polyethylene imine for 3 hours prior to use) under aspiration. Each of the filters was then washed four times with 5 ml of buffer (50 mM Tris-HCl pH 7.4, 5 mM MnCl 2 The radioactivity was counted by using Auto Gamma counter (Packerd RIASTAR 5420A). All data presented are specific binding defined as that displaceable by 3 uM unlabeled Substance P.

Further, the compound of the present invention, especially the compound is also superior in stability and the like.

EXAMPLES

5 The following Preparations and Examples are given for the purpose of illustrating the present invention in more detail.

to be continued on the next page WO 96/37489 PCT/JP96/01335 16 Preparation 1 To a mixture of N 2 -(tert-butoxycarbonyl)-N-formyl-Dtryptophan (3.99 g) and N-benzyl glycin benzyl ester hydrochloride (3.50 g) in dichloromethane (70 ml) was added triethylamine (5.85 ml) under nitrogen atmosphere. To the mixture was added 2 -chloro-l-methylpyridinium iodide (3.67 g) at room temperature, and the resulting mixture was stirred for 2 hours. After the reaction was completed, dichloromethane (30 ml) and water (30 ml) were added. The organic layer was separated, washed with 0.5N hydrochloric acid (10 ml), water (10 ml), aqueous sodium bicarbonate solution (10 ml) and brine (20 ml) successively and dried over magnesium sulfate. After evaporation of the solvent, the residue was purified on a silica gel column (140 g) eluting with a mixture of toluene and ethyl acetate to give (2R)-N-benzyl-N-benzyloxycarbonylmethyl-2-(tertbutoxycarbonylamino) (N-formyl-lH-indol-3-yl)propanamide (6.41 g) as an oil.

WO 96/37489 PCT/JP96/01335 17 IR (CHC1 3 3300, 2970, 1740, 1700, 1644, 1604 cm- 1 NMR (DMSO-d 6 5) 0.89, 1.22 and 1.29 (9H, 3 s); 2.80-3.10 (2H, 3.95-4.25 (2H, 4.40-4.90 (3H, 4.95-5.20 (2H, 7.05-7.75 (15H, m); 7.98 and 8.22 (1H, 2 br 9.22 and 9.61 (1H, 2 br s) MASS 570 (M+1) Preparation 2

HO

N

To an ice-cooled solution of the object compound of Preparation 1 (6.39 g) in dichloromethane (50 ml) was added 4N hydrogen chloride in dioxane solution (50 ml). The mixture was stirred at the same temperature for 30 minutes and at room temperature for 1 hour. After evaporation of the solvent, the residue was partitioned between dichloromethane ml) and aqueous sodium bicarbonate solution (30 ml). The organic layer was separated, dried over magnesium sulfate and filtered. To the filtrate was added triethylamine (1.67 ml) at room temperature, and the mixture was stirred for hours. After evaporation, the residue was triturated with diisopropyl ether, collected by filtration and dried to give (3R)-l-benzyl-3-(N-formyl-!H-indol-3-ylmethyl)piperazine-2,5dione (3.93 g).

WO 96/37489 PCT/JP96/01335 18 mp: 176-178 0

°C

IR (Nujol) 3250, 1709, 1648, 1630 cm-1 NMR (DMSO-d 6 5) 2.95-3.30 and 3.35-3.70 (4H, 2 m); 4.22 (1H, d, J=14.6Hz); 4.30-4.40 (1H, 4.54 (1H, d, J=14.9Hz); 6.80-7.75 (9H, 7.95-8.50 (2H, 9.20 and 9.65 (1H, 2 br s) PreDaration 3

H

0 To an ice-cooled solution of the object compound of Preparation 2 (3.89 g) in a mixture of methanol (175 ml) and tetrahydrofuran (50 ml) was added aqueous 0.1N sodium hydroxide solution (108 ml). The mixture was stirred at the same temperature for 30 minutes and at room temperature for 1.5 hours. After evaporation of the solvent, the residue was extracted with dichloromethane. The organic layer was washed with water and an aqueous sodium chloride solution, and dried over magnesium sulfate. Evaporation of the solvent gave (3R)-l-benzyl-3-(lH-indol-3-ylmethyl)piperazine-2,5-dione (3.68 g).

mp 207-208oC IR (Nujol) 3402, 1650 cm 1 NMR (DMSO-d 6 5) 2.68 (1H, d, J=17.2Hz); 3.04 (1H, dd, J=14.4 and 4.4Hz); 3.20-3.40 (2H, 4.24 (1H, 4.10-4.40 (2H, 6.75-7.60 (10H, 8.35 WO 96/37489 PCT/JP96/01335 19 (1H, 10.94 (1H, s) MASS 334 (M+1) Preparation 4

H

To a suspension of lithium aluminum hydride (0.77 g) in tetrahydrofuran (40 ml) was added dropwise a solution of the object compound of Preparation 3 (3.40 g) in tetrahydrofuran ml) at 0°C under nitrogen atmosphere. The mixture was stirred at room temperature for 50 minutes and at refluxing temperature for 1 hour. The resulting mixture was diluted with tetrahydrofuran (60 ml) and cooled to 0°C. Water ml) and aqueous 15% sodium hydroxide solution (0.8 ml) were added slowly. The resulting insoluble inorganic material was removed by filtration and washed with tetrahydrofuran. The filtrate and the washing were combined and evaporated under reduced pressure to give (3R)-!-benzyl-3-(lH-indol-3ylmethyl)piperazine (3.68 g) as an oil.

IR (CHC1 3 3240, 3040, 2900 cm- 1 NMR (DMSO-d 6 5) 1.70-2.00 and 2.30-2.45 (2H, 2 m); 2.50-3.00 (7H, 3.25-3.60 (3H, 6.80-7.60 10.80 (1H, s) MASS 306 (M+l) WO 96/37489 PCT/JP96/01335 20 Preparation

H

O

CF

3 CN

CF

3 To a mixture of 3,5-bis(trifluoromethyl)benzoic acid (1.15 g) and (3R)-l-benzyl-3-(lH-indol-3-ylmethyl)piperazine (1.61 g) in dichloromethane (80 ml) was added triethylamine (1.55 ml) at room temperature under nitrogen atmosphere.

2 -Chloro-1-methylpyridinium iodide (1.37 g) was added, and the mixture was stirred at room temperature for 2.5 hours.

The resulting mixture was poured into water (20 ml). The organic layer was washed successively with 0.5N hydrochloric acid, water, aqueous sodium bicarbonate solution and brine, and dried over magnesium sulfate. After evaporation under reduced pressure, the residue was chromatographed on silica gel with toluene ethyl acetate as an eluent to give (2R)-4-benzyl-!-[3,5-bis(trifluoromethyl)benzoyl]-2-(1Hindol-3-ylmethyl)piperazine (0.87 g) as a syrup.

IR (CHC! 3 3430, 3300, 3000, 2910, 2800, 1630-1610 cm- 1 NMR (DMSO-d 6 5) 1.90-2.40 (2H, 2.70-3.90 (8H, 4.25-4.40 and 4.75-4.90 (1H, 6.50-7.45 7.50-8.25 (3H, 10.77 (1H, s) MASS 546 (M+1) WO 96/37489 PCTIJP96O 1335 21 Preparation 6

H

eI_

K

0

CF

3

CF

3 A mixture of 2 R) -4-benzyl-l1 5bis (trif luoromethyl) benzoyl]-2- (lH-indol-3-ylmethyl)piperazine (5.20 ammonum formate (1.50 g) and 10%0 Pd charcoal (0.52 g) in ethanol ml) was refluxed for 7.5 hours under nitrogen atmosphere.

The reaction mixture was cooled to room temperature and filtered through Celite pad. The filtrate was concentrated under reduced pressure and the residue was purified on a silica gel column eluting with a mixture of dichioromethane and methanol (20:1) to give 2 benzoyl1-2-(lH-indol3-ymethyl)piperazin (2.67 g) as a syrup.

IR (CHCl 3 3280, 2900, 1622 cm- 1 NMR (DMSO-d 6 6) :2.50-3.50 (9H, mn); 3.6-4.8 (1H, in); 6.55-7.40 (5H, mn); 7.50-8.22 (3H, in); 10.84 (1H, s) MASS 456 (M+1) Preraration 7 A mixture of (2R)-1-[3,5-bis (trifluoromethyl)benzoyl.2- (lH-indol-3-ylinethyl)piperazine (1.5 benzyl 2 -bromoacetate (0.79 triethylamine (0.55 ml) and tetrahydrofuran (15 ml) was stirred overnight at room temperature. The resulting insoluble material was removed by filtration and the filtrate was concentrated under reduced WO 96/37489 WO 9637489PCTIJP96/0 1335 -22 pressure. The residue was purified by column chromatography on silica gel eluting with a mixture of dichloromethane and methanol (30:1) to give (benzyloxycarbonylmethyi)-lr3, 5-bis (trifluoroinethyl)benzoyij (lK-Hindol-3-yimethyl) piperazine (1.92 g) [cc] 21 11.6- MeCH) IR (Neat) :3600-3100, 1735, 1626, 1275, 1129, 900 crrC 1 NMIR (DMSO-d 6 5 2.20-5.20 (13H, mn); 6.60-8.20 (13H, mn); 10. 85 (1K, br s) MASS :604 454 Preiparation 8 A mixture of (2R) (benzyloxycarbonylmethyl) bi*s (trifluoromethyl)benzoylp.> (lK-inrdol-3-ylmethyl) piperazine (1.86 10% Pd charcoal (0.186 g) and tetrahydrofuran (93 ml) was stirred for 17 hours under hydrogen gas atmosphere (1 atm). The catalyst was removed by filtration and the filtrate was concentrated. The residue was triturated with ethyl ether to give (2R)-4- (carboxvinethyl)-l-[3,5-bi 5 (trifluoromethyl)benzojj...2.(1Kindol-3-ylinethyl)piperazine (0.83 g) as a white powder.

[c]19 3.0'

DMF)

LaD mp 152-156*C IR (Nujol) :3600-3100, 1654, 1630, 1277, 1196, 1130 cmJ 1 NMR (DMSO-d 6 2.20-5.20 (11H, mn); 6.60-8.20 (8H, mn); 10.85 (1H, s) MASS :514 (M+1) Prepoaration 9 To an ice-cooled mixture of (2R)-2-benzyl-1-[3,5bis (trifluoromethyl)benzoyllpiperazine (0.3 g) and triethylamine (0.39 ml) in dimethylformamide (8 ml) was added 3 -(chloromethyl)pyridine hydrochloride (0.12 g) The reaction mixture was stirred at the same temperature for WO 96/37489 PCT/JP96/01335 23 minutes and then at room temperature for 2 hours. Additional triethylamine (0.39 ml) and 3 -(chloromethyl)pyridine hydrochloride (0.12 g) were added and the resulting mixture was stirred overnight. The reaction mixture was filtered and the filtrate was concentrated and subjected to a chromatography on a silica gel eluting with a mixture of toluene and ethyl acetate The eluent was treated with 4N hydrogen chloride in ethyl acetate solution to give (2R)- 2-benzyl-1-[3,5-bis (trifluoromethyl)benzoyl]-4-(pyridin-3-ylmethyl)piperazine dihydrochloride.

mp 164-168°C [2] 5 +9.10 MeOH) IR (Nujol) 3700-3100, 2700-2000, 1630, 1270, 1120, 900 cm 1 NMR (DMSO-d 6 5) 2.80-5.40 (11H, 6.85-6.90 (1H, 7.10-7.40 (4H, 7.46 (1H, 7.75 (1H, s); 7.90-8.00 (1H, 8.19-8.23 (1H, 8.66-8.70 (1H, 8.88-8.91 (1H, 9.09 (1H, s) MASS 508 (free) Preparation 0 CF

C

F

3 WO 96/37489 PCT/JP96/01335 24 To a stirred mixture of (2R)-2-benzyl-1-[3,5bis(trifluoromethyl)benzoyl]piperazine (0.3 g) and 2-(1Hindol-3-yl)acetic acid (0.13 g) in dichloromethane (8 ml) containing triethylamine (0.25 ml) was added 2-chloro-lmethylpyridinium iodide (0.22 g) at room temperature under nitrogen atmosphere. After being stirred for 5 hours, the reaction mixture was diluted with dichloromethane and washed with 0.1N hydrochloric acid, aqueous saturated sodium bicarbonate solution and brine, and dried over magnesium sulfate. After removal of the solvent, the residue was purified by column chromatography on silica gel using chloroform-methanol (50:1) as eluent to give (2R)-2-benzyl-l- [3,5-bis(trifluoromethyl)benzoyl]-4-[2-(1H-indol-3yl)acetyl]piperazine (0.34 g) as a white powder.

mp 201-210°C [a]D 2 7 +27.6* MeOH) IR (Nujol) 3270, 1630, 1276, 1115, 900, 737 cm-1 NMR (DMSO-d 6 5) 2.60-5.00 (11H, 6.70-7.70 (12H, 8.10-8.20 (1H, 10.85-11.10 (1H, m) MASS 574 417 Preparation 11 To a solution of (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2-(lH-indol-3-ylmethyl)piperazine (0.1 g) in dichloromethane (10 ml) was added 4N hydrogen chloride in dioxane solution (0.05 ml) at 0°C. The resulting mixture was stirred at the same temperature for 50 minutes and then concentrated under reduced pressure. The obtained powder was collected by filtration and washed with ethyl ether to give (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2- (H-indol-3ylmethyl)piperazine hydrochloride (0.1 g).

IR (Nujol) 3340, 1648 cm- 1 NMR (DMSO-d 6 5) 2.9-3.9 (8H, 3.9-5.2 (1H, m); 6.57-7.50 (5H, 7.50-8.30 (3H, 9.40-10.00 (2H, 10.96 (1H, s) WO 96/37489 PCT/JP96/01335 25 MASS 456 (free) Preparation 12 To a stirred mixture of 2

R)-

4 -(2-aminoethyl)-1-[3,5bis(trifluoromethyl)benzoyl]-2-(3, 4 -dimethylbenzyl)piperazine dihydrochloride (110 mg), triethylamine (0.2 ml) in dichloromethane (10 ml) was added methanesulfonyl chloride (0.1 ml) at 0°C. After stirring for 1 hour, the reaction mixture was poured into ice-water and extracted with ethyl acetate. The extract was washed successively with aqueous saturated sodium bicarbonate solution and brine, and dried.

After evaporation of the solvent in vacuo, the residue was purified by column chromatography on a silica gel eluting with a mixture of dichloromethane and methanol (40:1) and treated with 4N hydrogen chloride in ethyl acetate solution to give 2 R)-l-f 3 ,5-bis(trifluoromethyl)benzoyl]-2-(3, 4 dimethylbenzyl)-4-[2-(mesylamino)ethyl]piperazine hydrochloride (50 mg).

mp >220°C [a] 22 +0.20

DMF)

IR (Nujol) 3350, 2700-2400, 1645, 1500, 1450, 1380 cm 1 NMR (DMSO-d 6 5) 2.10 and 2.18 (6H, 2 2.7-5.2 (17H, 6.6-7.7 (5H, 8.1-8.2 (1H, 11.05- 11.4 (1H, m) MASS 566 (free) Example 1 To a stirred solution of 2 R)-4-(carboxymethyl)-1-[3,5bis (trifluoromethyl)benzoyl]-2-(1H-indol-3-ylmethyl)piperazine (1 g) in dry dimethylformamide (10 ml) was added 1-hydroxybenzotriazole (0.29 g) and 1-(3dimethylaminopropyl)- 3 -ethylcarbodiimide hydrochloride (0.41 g) at room temperature. After stirring for 15 minutes at room temperature, 1-amino-4-methylpiperazine (320 mg) was WO 96/37489 PCT/JP96/01335 26 added and further stirred for 5 hours at the same temperature. The reaction mixture was poured into a solution of sodium hydrogencarbonate (1.8 g) in water (100 ml) and extracted three times with 20 ml portions of ethyl acetate.

The organic layers were combined and washed with brine ml). The organic layer was dried over magnesium sulfate and filtered and the solvent was removed by rotary evaporator.

The crude product was purified by chromatography (silica gel, dichloromethane:methanol, 5:1) to afford 2 5-bis(trifluoromethyl)benzoyl]-2-(1H-indol-3ylmethyl)-4-[N-(4-methyl-l-piperazinyl)carbamoylmethyl]piperazine (0.94 g) as a yellowish powder.

IR (Nujol) 3180, 1680, 1630, 1276, 1170, 1130, 1005, 897 cm-1 NMR (DMSO-d 6 5) 2.16 (3H, 2.0-5.0 (19H, m); 6.6-8.2 (8H, 8.47, 8.77 (1H, 2 s); 10.85 (1H, s) Example 2 (2R)-1-[3,5-Bis(trifluoromethyl)benzoyl]-2-(1H-indol-3ylmethyl)-4-[N-( 4 -methyl-l-piperazinyl)carbamoylmethyl]piperazine (10.89 g) and fumaric acid (2.07 g) were dissolved in ethanol (50 ml) at 70°C. After cooling, the resulting solution was concentrated under reduced pressure to give a powder (13.18 The powder (9.68 g) was dissolved in 2-butanone (194 ml) at reflux temperature and the solution was allowed to stir at room temperature to afford crystals, which was collected by filtration and dried to give (2R)-1- 5-bis(trifluoromethyl)benzoyl]-2- (H-indol-3-ylmethyl)-4- 4 -methyl-l-piperazinyl)carbamoylmethyl]piperazine fumarate (7.94 g).

mp 169.5-171°C IR (Nujol) 3220, 1700, 1653, 1630, 1275, 1217, 1168, 1122, 979, 894, 730 cm- 1 NMR (DMSO-d 6 5) 2.23, 2.26 (3H, 2 2.10-4.93 WO 96/37489 WO 9637489PCT/JP96/01335 27- (19K, in); 6.60 (2H, 6.54-8.23 (8H, mn); 8.50, 8.85 (1H, 2 s) 10.85 (1H, s) Example 3 Compound-(If), namely 2 benzoyl] (lK-irdol-3-ylmethyl) (4-methyl-ipiperazinyl) carbamoylmethylipipoerazine fumarate was also obtained according to the following scheme.

CH

3 0H

H

2

N_''CO

2

H

ClCH 2 000l MID 224-225 0C (dec)

H

ClN K C 2

HK

mD 243 0 C (dec)

CK

2

NK

2 WO 96/37489 WO 9637489PCT/JP96/01335 -28

H

N

0 CH 3

CO

2

H

0 mp: 11iC LiA1H 4

H

I2HCJ.

HN I mp 221 0

C

)2 H

N

CF

3 /i~i

F

3 0 Pd charcoal

HCO

2

NH

2

H

N N

:F

3 ,N NNH BrCH 2

CO

2

C

2

H

WO 96/37489 WO 9637489PCTIJP96O 1335 29 NaOH MlP 152-156'C 1) cJCo 2 CH (CH 3 2 2) H N- 1 cH 3 Compound (I) H0 2

CCH

~HCCOU

2

H

2 -butanone

F

3

HO

2

CCH

HC11 Compound (If) WO 96/37489 PCT/JP96/01335 30 Example 4 A mixture of (2R)-1-[3,5-bis(trifluoromethyl)benzoyl]-2- (1H-indol-3-ylmethyl)piperazine (120 mg), 4-chloromethyl-2- (2-methoxyethylcarbonylamino)thiazole (70 mg) and powdered sodium hydrogen carbonate (27 mg) in dry dimethylformamide was stirred for 5 hours and 20 minutes at 60°C. The reaction mixture was powered into water and the resulting precipitate was collected by filtration. The crude product was purified by column chromatography on silica gel eluting with a mixture of dichloromethane and methanol The eluate was evaporated under reduced pressure and treated with 17.6% hydrogen chloride in ethanol (0.12 ml) to give bis(trifluoromethyl)benzoyl]-2-(1H-indol-3-ylmethyl)-4-[[2- (3-methoxypropanoylamino)thiazol-4-yl]methyl]piperazine hydrochloride (140 mg).

[a] 22 -34.0* MeOH) IR (Nujol) 3650-3100, 2750-2000, 1635, 1275, 1130, 900 cm-1 NMR (DMSO-d 6 5) 2.60-5.20 (18H, 6.60-8.21 (9H, 10.90-11.00 (1H, 11.20-12.00 (1H, 12.19 (1H, s) MASS 654 (free) Example The following piperazine derivatives (Table 1) were prepared by the similar manner to that of the each Example No. or Preparation No. defined in the "Process" column. The physical properties of the object compounds are shown after the table.

WO 96/37489 WO 9637489PCTIJP96O 1335 31 Table 1 Example Object Compounds ___Starting No.

-A-R

4 Salt CompoundPrcs -CH N Pr. (Pre- 5-1) )NHCOCH 3 HC1 paration) Ex. 4 S 6 5-2) -C 2 N\ NHCOOC2H HC1 Pr. 6 Ex. 4

S

-CH

5-3) 2 T, Y-NHCO--4'\ HC1 Pr. 6 Ex. 4 H HO(CH 3 )3 HC1 Pr.. 6 Ex. 4

S

-C2 N NC-<HC1 Pr. 6 Ex. 4

S

WO 96/37489 WO 9637489PCTIJP96O 1335 32 Table 1 (continued) WO 96/37489 -33 Table 1 (continued PCT/JP96101335 EapeObject Compounds Starting No.

P

4 Salt CompoundPrcs 5-15) 7F2- T' HCl Pr. 6 Ex. 4

NHCOCH

3 5-16) C2 N HC1 Pr. 6 Ex. 4 S N-COC 2 H

H

Physical properties of the compounds of the Example Exampole 5-1) mp :185-189*C [ajB 4 30.20 MeOK) 1K (Nujol) 3660-3100, 2800-2000, 1635, 1545, 1276, 1183, 1130, 900 cm- 1 NNR (DMSO-d 6 6) :1.36-5.10 (14K, in); 6.59-8.22 in); 10.90-11.00 (1H, in); 12.15 (1K, s) MASS 610 (free), 456 Examnle- 5-2) []22 -33.40 MeOK)

~~D

!R (Nujol) 3650-3100, 2800-2000, 1715, 1635, 1555, 1274, 1130, 900 cm- 1 NMK (DMSO-d 6 65) :1.25 (3K, t, 2.73-5.10 (13K, in); 6.60-8.30 (9H, in); 10.90-11.00 (1K, in); 11.81 (1K, br s) MASS :640 (free), 456 Exaame5-3)L I(I22 -42.0* MeOK) [aD IK (Nujol) 3600-3100, 2750-2000, 1635, 1540, 1277, WO 96/37489 WO 9637489PCT/JP96/01335 -34 1175, 1130 cin- 1 NMR (DM0-dl, :2.73-5.20 (11H, in); 6.59-8.21 (14H, mn); 10.90-11.00 (1H, In); 12.69 s) MASS 672 (free), 456 Examp~le 5-4) [a]22 -24.2* MeOH) IR (Nujol) 3650-3100, 2750-2000, 1635, 1540, 1276, 1170, 1129, 900 cmJ 1 NMR (DMSO-d 6 5) :1.23 (9H, 2.73-5.10 (liH, in); 6.50-8.20 (9H, mn); 10.80-11.00 (111, mn); 11.84 (1H,

S)

MASS :652 (free), 456 Examp~le [a]22 -35.8* MeQH) IR (Nujol) 3650-3100, 2750-2000, 1635, 1540, 1276, 1170, 1130, 900 cin 1 NMR (DMSO-d 6 :0.80-1.00 (4H, mn); 1.90-2.00 (iH, mn); 2.73-5.15 (11H, in); 6.60-8.21 (9H, in); 10.90- 11.00 (iH, mn); 12.43 (1H, mn) MASS :636 (free), 456 Examp~le 5-6) 25[a]22 MeOH) IR (Nujol) 3650-3100, 2750-2000, 1635, 1542, 1275, 1170, 1131, 900 cin 1 NMR (DMSO-d 6 :0.89 (3H, t, J=7.4Hz); 1.56-1.67 (2H, mn); 2.40-2.50 (2H, in); 2.73-5.15 (liH, mn); 6.56-8.21 mn); 10. 90-10.94 (1H, mn); 12.12 (1H,

S)

MASS :638 (free) Example 5-7) la2 34.* MeOH) WO 96/37489 WO 9637489PCTIJP96O 1335 35 IR (Nujol) 3650-3100, 2750-2000, 1635, 1543, 1277, 1170, 1130, 900 cm-1 NMR (DMSO-d 6 5) :1.08 (3H, t, J=7.4Kz); 2.43-2.50 (2H, in); 2.73-5.15 (11H, mn); 6.55-8.21 (9H, mn); 10.90-10.94 (1H, in); 12.11 (1H, s) MASS :624 (free) [22 -15.20 MeOK) (Nujol) 3600-3100, 2750-2000, 1635, 1278, 1172, 1130, 900 cm- 1 NMR (DMSO-d 6 2.73-5.20 (11H, mn); 6.60-8.52 (11K, mn); 10.94 (1H, s) MASS 596 (free), 456 [22 -2o.6* MeaN) [aD IR (Nujol) 3650-3100, 2750-2000, 1635, 1276, 1170, 1129, 900 c- NMR (DMSO-d 6 5) :2.09 (3K, 2.73-5.20 (11K, mn); 6.60-8.20 (11K, mn); 10.46 (1K, 10.91 (1K, s) MASS :604 (free) Examule 5-10) 25[a]22 -13.0* MeOK) IR (Nujol) 3650-3050, 2750-2000, 1685, 1636, 1524, 1275, 1130, 900 c- NMR (DMSO-d 6 5) :1.31 (3K, t, J=6.5Kz); 2.24 (3K, 2.73-5.20 (13K, mn); 6.66-8.25 (8K, in); 10.94 (1K, 12.71 (1K, s) MASS :682 (free) Examp~le 5-11) (a18 -20.8* C05 eH [aD eK IR (Neat) 3700-3000, 1615, 1515, 1272, 1125, WO 96/37489 WO 9637489PCT/JP96/01335 -36 900 cnf 1 NMR (DMSO-d 6 2.00-5.00 (13H, in); 6.38-8.20 (9H, mn); 10.80 (1H, s) MASS 568 456 Examle 5-12) [(x]2 2 -10.4- MeOH) (Nujol) 3650-3100, 2750-2000, 1635, 1277, 1130 c- NM? (D-MSO-d 6 5) 3.00-5.20 (ilH, mn); 6.60-8.30 (ilK, mn); 10.95 (1K, s) MASS 568 (MUl) (free), 456 Exain~le 5-13) c2I 2 -31.*9 MeOH) (Nujol) 3270, 2750-2000, 1637, 1531, 1279, 1124, 964 c- NM?. (DMSO-d 6 5) 2.73-5.15 (i4H, in); 6.60-8.25 mn); 10.89 (iH, s) MASS 646 (free), 568, 456 Exainle 5-14) [cc 23 11.80 MeOH) IR (Nujol) 3650-3100, 1625, 1543, 1275, 1130 cirJ 1 NM?. (D-MSO-d 6 5) :2.09-2.11 (3H, in); 2.52-5.00 (11H, mn), 6.63-8.20 (9H, mn); 10.85 (lH, 12.07 (1H, s) MASS :638 456 Exainole 5-15) 51.60 NeOH) (Nujol) 3650-3100, 2750-2000, 1634, 1540, 1274, 1170, 1127, 900 cmJ 1 NM?. (D-MSO-d 6 5) :2.31 (3K, 2.73-5.35 (1lH, in); 6.63-8.25 (8K, mn); 10.94-11.00 (1K, in); 13.20 (1H, MASS 611 (free) Example 5-16) [cc] 22 -23.4° MeOH) IR (Nujol) 3650-3000, 2750-2000, 1620, 1274, 1175, 1128, 900 cm

I

NMR (DMSO-d6, 5) 1.10 (3H, t, J=7.2Hz), 2.60-5.10 (16H, m) 6.50-8.21 (9H, m) 10.91 (1H, s) 11.50- 11.90 (1H, br s) MASS 638 (free) Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and 1 5 variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

*e *eeoc

:Q-

*e *3O oo*

Claims (10)

1. A compound of the formula: C C CC *g C C C C C CCC. C. C g~* CCC C or fuinaric acid salt thereof.

2. The compound of claim 1, which is the compound of the formula: C CCC C C C CC C .C C C. CC g C C C C CC C C C C tgggg g C C H0 2 CCH 11 HCC 02 H WO 96/37489 PCT/JP96/01335 39

3. A process for preparing a compound of the formula F 3 C or fumaric acid salt thereof, which comprises reacting a compound of the formula 'OH or its reactive derivative at the carboxy group or a salt thereof with a compound of the formula WO 96/37489 PCT/JP96/01335 40 H 2 N-N/rNCH 3 or its reactive derivative at the amino group or a salt thereof to give a compound of the formula or fumaric acid salt thereof, or reacting a compound of the formula or a salt thereof other than fumaric acid salt thereof with fumaric acid to give a compound of the formula HO 2 CCH HCCO2H 0 NCH 3 NC~~N H S. *e S S S. 0 5*O S

4. A pharmaceutical composition comprising a compound of claim 1 as an active ingredient, in association with a charmaceutically acceptable, substantially non-toxic carrier or excipient.

5. A method for treating or preventing Tachykinin-mediated diseases which comprises administering an effective amount of a compound of claim 1 to human being or animals.

6. A compound of claim 1 when used as a medicament.

7. Use of a compound of claim 1 for manufacture of a medicament for treating or preventing Tachykinin- mediated diseases.

8. A compound of the following general formula SS S 5 S *2 S S S S. OS 55 S S S 55 5 S 555555 S F- EDITORIAL NOTE NUMBER 57031/96 THIS SPECIFICATION DOES NOT CONTAIN A PAGE NUMBERED "42" Q:\OPER\MJC\57031-97.SPE 1213/99 43

9. A method f or the manufacture of a medicament including the step of bringing a compound of claim 1 into a form suitable for administration.

10. A process according to claim 3 substantially as hereinbefore described with reference to any one of the examples. DATED this 12th day of March, 1999 3.Fujisawa Pharmaceutical Co., Ltd. By DAVIES COLLISON CAVE Patent Attorneys for the Applicant