AU762992B2

AU762992B2 - Tricyclic pyrazole derivatives

Info

Publication number: AU762992B2
Application number: AU19091/00A
Authority: AU
Inventors: Lee D. Arnold; Kevin J. Doyle; Anna M. Ericsson; Michael Hockley; Paul Rafferty; Robert W. Steele; David J. Wilkins
Original assignee: BASF SE
Current assignee: Abbott GmbH and Co KG
Priority date: 1998-11-06
Filing date: 1999-11-04
Publication date: 2003-07-10
Anticipated expiration: 2019-11-04
Also published as: TR200102277T2; HUP0200310A2; ZA200103610B; IL142584A0; SK5282001A3; CN1335836A; AU1909100A; EP1127051A2; HK1042895A1; KR20010086005A; WO2000027822A3; ID30132A; BG105481A; HUP0200310A3; JP2003517447A; WO2000027822A2; CA2350235A1; NO20012219D0; CZ20011563A3; BR9915132A

Description

WO 00/27822 PCT/US99/26105 1 TRICYCLIC PYRAZOLE DERIVATIVES This invention relates to certain 3-aryl or 3-heteroaryl pyrazoles with 4,5(3,4)-bicyclic ring fusion which are inhibitors of protein kinases, particularly tyrosine kinases and serine/threonine kinases, of which some are novel compounds, to pharmaceutical compositions containing these pyrazoles and to processes for preparing these pyrazoles.

BACKGROUND OF THE INVENTION There are at least 400 enzymes identified as protein kinases. These enzymes catalyze the phosphorylation of target protein substrates. The phosphorylation is usually a transfer reaction of a phosphate group from ATP to the protein substrate.

The specific structure in the target substrate to which the phosphate is transferred is a tyrosine, serine or threonine residue. Since these amino acid residues are the target structures for the phosphoryl transfer, these protein kinase enzymes are commonly referred to as tyrosine kinases or serine/threonine kinases.

The phosphorylation reactions, and counteracting phosphatase reactions, at the tyrosine, serine and threonine residues are involved in countless cellular processes that underlie responses to diverse intracellular signals (typically mediated through cellular receptors), regulation of cellular functions, and activation or deactivation of cellular processes. A cascade of protein kinases often participate in intracellular signal transduction and are necessary for the realization of these cellular processes. Because of their ubiquity in these processes, the protein kinases can be found as an integral part of the plasma membrane or as cytoplasmic enzymes or localized in the nucleus, often as components of enzyme complexes. In many instances, these protein kinases are an essential element of enzyme and structural protein complexes that determine where and when a cellular process occurs within a cell.

Protein Tyrosine Kinases. Protein tyrosine kinases (PTKs) are enzymes which catalyse the phosphorylation of specific tyrosine residues in cellular proteins.

This post-translational modification of these substrate proteins, often enzymes themselves, acts as a molecular switch regulating cell proliferation, activation or WO 00/27822 PCT/US99/26105 2 differentiation (for review, see Schlessinger and Ulrich, 1992, Neuron 9:383-391).

Aberrant or excessive PTK activity has been observed in many disease states including benign and malignant proliferative disorders as well as diseases resulting from inappropriate activation of the immune system autoimmune disorders), allograft rejection, and graft vs. host disease. In addition, endothelial-cell specific receptor PTKs such as KDR and Tie-2 mediate the angiogenic process, and are thus involved in supporting the progression of cancers and other diseases involving inappropriate vascularization diabetic retinopathy, choroidal neovascularization due to age-related macular degeneration, psoriasis, arthritis, retinopathy of prematurity, infantile hemangiomas).

Tyrosine kinases can be of the receptor-type (having extracellular, transmembrane and intracellular domains) or the non-receptor type (being wholly intracellular).

Receptor Tyrosine Kinases (RTKs). The RTKs comprise a large family of transmembrane receptors with diverse biological activities. At present, at least nineteen (19) distinct RTK subfamilies have been identified. The receptor tyrosine kinase (RTK) family includes receptors that are crucial for the growth and differentiation of a variety of cell types (Yarden and Ullrich, Ann. Rev. Biochem.

57:433-478, 1988; Ullrich and Schlessinger, Cell 61:243-254, 1990). The intrinsic function of RTKs is activated upon ligand binding, which results in phosphorylation of the receptor and multiple cellular substrates, and subsequently in a variety of cellular responses (Ullrich Schlessinger, 1990, Cell 61:203-212). Thus, receptor tyrosine kinase mediated signal transduction is initiated by extracellular interaction with a specific growth factor (ligand), typically followed by receptor dimerization, stimulation of the intrinsic protein tyrosine kinase activity and receptor transphosphorylation. Binding sites are thereby created for intracellular signal transduction molecules and lead to the formation of complexes with a spectrum of cytoplasmic signaling molecules that facilitate the appropriate cellular response cell division, differentiation, metabolic effects, changes in the extracellular microenvironment); see Schlessinger and Ullrich, 1992, Neuron 9:1-20.

Proteins with SH2 (src homology or phosphotyrosine binding (PTB) domains bind activated tyrosine kinase receptors and their substrates with high WO 00/27822 PCT/US99/26105 3 affinity to propagate signals into cells. Both of the domains recognize phosphotyrosine (Fantl et al., 1992, Cell 69:413-423; Songyang et al., 1994, Mol.

Cell. Biol. 14:2777-2785; Songyang et al., 1993, Cell 72:767-778; and Koch et al., 1991, Science 252:668-678; Shoelson, Curr. Opin. Chem. Biol. (1997), 227- 234; Cowbum, Curr. Opin. Struct. Biol. (1997), 835-838). Several intracellular substrate proteins that associate with receptor tyrosine kinases (RTKs) have been identified. They may be divided into two principal groups: substrates which have a catalytic domain; and substrates which lack such a domain but serve as adapters and associate with catalytically active molecules (Songyang et al., 1993, Cell 72:767-778). The specificity of the interactions between receptors or proteins and SH2 or PTB domains of their substrates is determined by the amino acid residues immediately surrounding the phosphorylated tyrosine residue. For example, differences in the binding affinities between SH2 domains and the amino acid sequences surrounding the phosphotyrosine residues on particular receptors correlate with the observed differences in their substrate phosphorylation profiles (Songyang et al., 1993, Cell 72:767-778). Observations suggest that the function of each receptor tyrosine kinase is determined not only by its pattern of expression and ligand availability but also by the array of downstream signal transduction pathways that are activated by a particular receptor as well as the timing and duration of those stimuli. Thus, phosphorylation provides an important regulatory step which determines the selectivity of signaling pathways recruited by specific growth factor receptors, as well as differentiation factor receptors.

Several receptor tyrosine kinases, and growth factors that bind thereto, have been suggested to play a role in angiogenesis, although some may promote angiogenesis indirectly (Mustonen and Alitalo, J. Cell Biol. 129:895-898, 1995).

One such receptor tyrosine kinase, known as "fetal liver kinase 1" (FLK-1), is a member of the type III subclass of RTKs. An alternative designation for human FLK-1 is "kinase insert domain-containing receptor" (KDR) (Terman et al., Oncogene 6:1677-83, 1991). Another alternative designation for FLK-1/KDR is "vascular endothelial cell growth factor receptor 2" (VEGFR-2) since it binds VEGF with high affinity. The murine version of FLK-1/VEGFR-2 has also been called NYK (Oelrichs et al, Oncogene 8(1):11-15, 1993). DNAs encoding mouse, rat and WO 00/27822 PCT/US99/26105 4 human FLK-1 have been isolated, and the nucleotide and encoded amino acid sequences reported (Matthews et al., Proc. Natl. Acad. Sci. USA, 88:9026-30, 1991; Terman et al., 1991, supra; Terman et al., Biochem. Biophys. Res. Comm. 187:1579- 86, 1992; Sarzani et al., supra; and Millauer et al., Cell 72:835-846, 1993).

Numerous studies such as those reported in Millauer et al., supra, suggest that VEGF and FLK-1/KDR/VEGFR-2 are a ligand-receptor pair that play an important role in the proliferation of vascular endothelial cells, and formation and sprouting of blood vessels, termed vasculogenesis and angiogenesis, respectively.

Another type III subclass RTK designated "frns-like tyrosine kinase-1" (Fit- 1) is related to FLK-1/KDR (DeVries et al. Science 255;989-991, 1992; Shibuya et al., Oncogene 5:519-524, 1990). An alternative designation for Flt-1 is "vascular endothelial cell growth factor receptor 1" (VEGFR-1). To date, members of the FLK-1/ KDR/VEGFR-2 and Flt-l/ VEGFR-1 subfamilies have been found expressed primarily on endothelial cells. These subclass members are specifically stimulated by members of the vascular endothelial cell growth factor (VEGF) family ofligands (Klagsburn and D'Amore, Cytokine Growth Factor Reviews 7: 259- 270, 1996). Vascular endothelial cell growth factor (VEGF) binds to Flt-1 with higher affinity than to FLK-1/KDR and is mitogenic toward vascular endothelial cells (Terman et al., 1992, supra; Mustonen et al. supra; DeVries et al., supra). Flt- 1 is believed to be essential for endothelial organization during vascular development. Fit-1 expression is associated with early vascular development in mouse embryos, and with neovascularization during wound healing (Mustonen and Alitalo, supra). Expression of Flt-1 in adult organs such as kidney glomeruli suggests an additional function for this receptor that is not related to cell growth (Mustonen and Alitalo, supra).

As previously stated, recent evidence suggests that VEGF plays a role in the stimulation of both normal and pathological angiogenesis (Jakeman et al., Endocrinology 133: 848-859, 1993; Kolch et al., Breast Cancer Research and Treatment 36: 139-155, 1995; Ferrara et al., Endocrine Reviews 18(1); 4-25, 1997; Ferrara et al., Regulation of Angiogenesis (ed. L. D. Goldberg and E.M. Rosen), 209-232, 1997). In addition, VEGF has been implicated in the control and enhancement of vascular permeability (Connolly, et al., J. Biol. Chem. 264: 20017- WO 00/27822 PCT/US99/26105 20024, 1989; Brown et al., Regulation ofAngiogenesis (ed. L.D. Goldberg and E.M.

Rosen), 233-269, 1997).

Different forms of VEGF arising from alternative splicing ofmRNA have been reported, including the four species described by Ferrara et al. Cell.

Biochem. 47:211-218, 1991). Both secreted and predominantly cell-associated species of VEGF have been identified by Ferrara et al. supra, and the protein is known to exist in the form of disulfide linked dimers.

Several related homologs ofVEGF have recently been identified. However, their roles in normal physiological and disease processes have not yet been elucidated. In addition, the members of the VEGF family are often coexpressed with VEGF in a number of tissues and are, in general, capable of forming heterodimers with VEGF. This property likely alters the receptor specificity and biological effects of the heterodimers and further complicates the elucidation of their specific functions as illustrated below (Korpelainen and Alitalo, Curr. Opin. Cell Biol., 159- 164, 1998 and references cited therein).

Placenta growth factor (PIGF) has an amino acid sequence that exhibits significant homology to the VEGF sequence (Park et al., J. Biol. Chem. 269:25646- 54, 1994; Maglione et al. Oncogene 8:925-31, 1993). As with VEGF, different species of P1GF arise from alternative splicing ofmRNA, and the protein exists in dimeric form (Park et al., supra). P1GF-1 and PIGF-2 bind to Flt-1 with high affinity, and P1GF-2 also avidly binds to neuropilin-1 (Migdal et al, J. Biol. Chem.

273 22272-22278), but neither binds to FLK-1/KDR (Park et al., supra). P1GF has been reported to potentiate both the vascular permeability and mitogenic effect of VEGF on endothelial cells when VEGF is present at low concentrations (purportedly due to heterodimer formation) (Park et al., supra).

VEGF-B is produced as two isoforms (167 and 185 residues) that also appear to bind Flt-1/VEGFR-1. It may play a role in the regulation of extracellular matrix degradation, cell adhesion, and migration through modulation of the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1 (Pepper et al, Proc. Natl. Acad. Sci. U. S. A. (1998), 95(20): 11709-11714).

VEGF-C was originally cloned as a ligand for VEGFR-3/Flt-4 which is primarily expressed by lymphatic endothelial cells. In its fully processed form, WO 00/27822 PCT/US99/26105 6 VEGF-C can also bind KDR/VEGFR-2 and stimulate proliferation and migration of endothelial cells in vitro and angiogenesis in in vivo models Lymboussaki et al, Am. J. Pathol. (1998), 153(2): 395-403; Witzenbichler et al, Am. J. Pathol. (1998), 153(2), 381-394). The transgenic overexpression of VEGF-C causes proliferation and enlargement of only lymphatic vessels, while blood vessels are unaffected.

Unlike VEGF, the expression of VEGF-C is not induced by hypoxia (Ristimaki et al, J. Biol. Chem. (1998), 273(14),8413-8418).

The most recently discovered VEGF-D is structurally very similar to VEGF- C. VEGF-D is reported to bind and activate at least two VEGFRs, VEGFR-3/Flt-4 and KDR/VEGFR-2. It was originally cloned as a c-fos inducible mitogen for fibroblasts and is most prominently expressed in the mesenchymal cells of the lung and skin (Achen et al, Proc. Natl. Acad. Sci. U. S. A. (1998), 95(2), 548-553 and references therein).

VEGF-C and VEGF-D have been claimed to induce increases in vascular permeability in vivo in a Miles assay when injected into cutaneous tissue (PCT/US97/14696; W098/07832, Witzenbichler et al., supra). The physiological role and significance of these ligands in modulating vascular hyperpermeability and endothelial responses in tissues where they are expressed remains uncertain.

Based upon emerging discoveries of other homologs of VEGF and VEGFRs and the precedents for ligand and receptor heterodimerization, the actions of such VEGF homologs may involve formation of VEGF ligand heterodimers, and/or heterodimerization of receptors, or binding to a yet undiscovered VEGFR (Witzenbichler et al., supra). Also, recent reports suggest neuropilin-1 (Migdal et al, supra) or VEGFR-3/Flt-4 (Witzenbichler et al., supra), or receptors other than KDR/VEGFR-2 may be responsible for the induction of vascular permeability (Stacker, Vitali, Domagala, Nice, and Wilks, "Angiogenesis and Cancer" Conference, Amer. Assoc. Cancer Res., Jan. 1998, Orlando, FL; Williams, Diabetelogia 40: S118-120 (1997)). Until now, no direct evidence for the essential role of KDR in VEGF-mediated vascular hyperpermeability has been disclosed.

The Non-Receptor Tyrosine Kinases. The non-receptor tyrosine kinases represent a collection of cellular enzymes which lack extracellular and WO 00/27822 PCT/US99/26105 7 transmembrane sequences. At present, over twenty-four individual non-receptor tyrosine kinases, comprising eleven (11) subfamilies (Src, Frk, Btk, Csk, Abl, Fes/Fps, Fak, Jak, Ack and LIMK) have been identified. At present, the Src subfamily of non-receptor tyrosine kinases is comprised of the largest number of PTKs and include Src, Yes, Fyn, Lyn, Lck, Blk, Hck, Fgr and Yrk. The Src subfamily of enzymes has been linked to oncogenesis. A more detailed discussion of non-receptor tyrosine kinases is provided in Bolen, 1993, Oncogene 8:2025-2031, which is incorporated herein by reference.

Many of the tyrosine kinases, whether an RTK or non-receptor tyrosine kinase, have been found to be involved in cellular signaling pathways involved in numerous pathogenic conditions, including cancer, psoriasis, and other hyperproliferative disorders or hyper-immune responses.

Development of Compounds to Modulate the PTKs. In view of the surmised importance of PTKs to the control, regulation, and modulation of cell proliferation, the diseases and disorders associated with abnormal cell proliferation, many attempts have been made to identify receptor and non-receptor tyrosine kinase "inhibitors" using a variety of approaches, including the use of mutant ligands (U.S.

Application No. 4,966,849), soluble receptors and antibodies (Application No. WO 94/10202; Kendall Thomas, 1994, Proc. Natl. Acad. Sci 90:10705-09; Kim et al., 1993, Nature 362:841-844), RNA ligands (Jellinek, et al., Biochemistry 33:10450- 56; Takano, et al., 1993, Mol. Bio. Cell 4:358A; Kinsella, et al. 1992, Exp. Cell Res.

199:56-62; Wright, et al., 1992, J. Cellular Phys. 152:448-57) and tyrosine kinase inhibitors (WO 94/03427; WO 92/21660; WO 91/15495; WO 94/14808; U.S. Patent No. 5,330,992; Mariani, et al., 1994, Proc. Am. Assoc. Cancer Res. 35:2268).

More recently, attempts have been made to identify small molecules which act as tyrosine kinase inhibitors. For example, bis monocyclic, bicyclic or heterocyclic aryl compounds (PCT WO 92/20642) and vinylene-azaindole derivatives (PCT WO 94/14808) have been described generally as tyrosine kinase inhibitors. Styryl compounds Patent No. 5,217,999), styryl-substituted pyridyl compounds Patent No. 5,302,606), certain quinazoline derivatives (EP Application No. 0 566 266 Al; Expert Opin. Ther. Pat. (1998), 475-478), selenoindoles and selenides (PCT WO 94/03427), tricyclic polyhydroxylic WO 00/27822 PCT/US99/26105 8 compounds (PCT WO 92/21660) and benzylphosphonic acid compounds (PCT WO 91/15495) have been described as compounds for use as tyrosine kinase inhibitors for use in the treatment of cancer. Anilinocinnolines (PCT W097/34876) and quinazoline derivative compounds (PCT W097/22596; PCT W097/42187) have been described as inhibitors of angiogenesis and vascular permeability.

In addition, attempts have been made to identify small molecules which act as serine/threonine kinase inhibitors. In particular, bis(indolylmaleimide) compounds have been described as inhibiting particular PKC serine/threonine kinase isoforms whose dysfunction is associated with altered vascular permeability in VEGF-related diseases (PCT W097/40830; PCT W097/40831).

The identification of effective small compounds which specifically inhibit signal transduction by modulating the activity of receptor and non-receptor tyrosine and serine/threonine kinases to regulate and modulate abnormal or inappropriate cell proliferation, differentiation, or metabolism is therefore desirable. In particular, the identification of methods and compounds that specifically inhibit the function of a tyrosine kinase which is essential for angiogenic processes or the formation of vascular hyperpermeability leading to edema, ascites, effusions, exudates, and macromolecular extravasation and matrix deposition as well as associated disorders would be beneficial.

SUMMARY OF THE INVENTION The present invention provides a method of inhibiting the kinase activity of tyrosine kinases and serine/threonine kinases comprising the administration of a compound represented by formula I: R3 R4 X R, R\W N R N R, R, WO 00/27822 PCT/US99/26105 9 and pharmaceutically acceptable salts thereof in which X represents a) substituted methylene carbonyl, c) oxygen d) a group of formula -C=NOR7 in which R7 represents H or a C1-4 alkyl group, e) a group of formula NR8 in which R8 represents H, an optionally substituted C1-4 alkyl group or optionally substituted phenyl, f) a group of formula (CH 2 in which n is 1, 2 or 3, or g) a group of formula S(O)p in which p is 0, 1 or 2; R, represents H

R

2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C,.

6 alkoxy group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group a phenyl C1-6 alkyl WO 00/27822 PCT/US99/26105 group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1 -6 alkyl group, a C1 -6 alkoxy group or halo, with the provisos that 1) when X represents carbonyl or a substituted methylene, R1 is hydrogen and R3, R4, R5 and R6 each represent hydrogen, then R2 is not pyridyl, phenyl or phenyl substituted by C1-2 alkyl, a halogen atom, a lower alkoxy group, a hydroxyl group or an amino group; and 2) when X is a group of formula (CH 2 in which n is 1, 2 or 3, R1 is hydrogen and two of R3, R4, R5 and R6 independently represent hydrogen, halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl and the other two represent hydrogen, then R2 is not thienyl, furyl, pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having two or less substituents wherein the substituents are halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl; to said kinase in sufficient concentration to inhibit the enzyme activity of said kinase.

WO 00/27822 PCT/US99/26105 11 In further embodiments, R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C.6 alkoxy group optionally substituted by one or more of the following: hydroxy, a C 16 alkoxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C 1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group, (C 3

-C

6 )heterocycloalkyl-(C 0 -C,)alkyl (heterocycloalkyl pyridine, morpoline, piperazine, and N-methylpiperazine) or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group a phenyl C1-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally WO 00/27822 PCT/US99/26105 12 substituted by one or more of the following: a C1-6 alkyl group, a C1-6 alkoxy group or halo.

Certain compounds of formula I are known in the literature. Compounds of formula I in which X represents methylene, ethylene, trimethylene, vinylene, substituted methylene are disclosed in US 3,932,430, US 3,843,665 and US 3,848,666. Compounds in which X represents carbonyl are disclosed in JP 130521. Compounds in which X represents O and R1, R3, R4, R5 and R6 represent hydrogen and R2 represents phenyl, 2,4-dimethylphenyl, 2-thienyl, 3thienyl and 2-pyrrolyl are disclosed in J. Het. Chem. 1984, 21 937-943, J. Org.

Chem. 1972, 37 (15) 2402 and JHet Chem. 1971, 855-859. A compound in which X represents S and R1, R3, R4, R5 and R6 each represent hydrogen and R2 represents 2,4-dichlorophenyl is disclosed in Monatsh Chem. 1974, 105, 869. A compound of formula I in which X represents SO2 and R1, R3, R4, R5 and R6 each represent hydrogen and R2 represents phenyl is disclosed in Liebigs Ann. Chem.

1974, 1248.

W097/15308 discloses that 3-(4-methylphenyl)indeno[1,2-c]pyrazol-4(1H)one oxime may be used to treat bone deficit conditions. 3-(3,4-Dimethoxyphenyl)indeno[1,2-c]pyrazol-4(1H)-one oxime is commercially available.

Certain fused pyrazoles of formula I are commercially available but no pharmacological activity has been disclosed for these compounds. Compounds which are commercially available are as follows: 3-(2-thienyl)indeno[1,2-c]pyrazol-4(1H)-one and 3-phenyl-lH-benzofuro[3,2-c]pyrazole.

In another aspect, the present invention provides a first group of novel compounds of formula I and pharmaceutically acceptable salts thereof in which: X represents a group of formula S(O)p in which p represents 0,1 or 2; R, represents H;

R

2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and

R

3 R4, R5 and R6 are as previously defined; with the provisos that WO 00/27822 PCT/US99/26105 13 1) when R1, R3, R4, R5 and R6 each represent hydrogen and X represents SO 2 then R 2 does not represent phenyl and 2) when X represents S and RI, R3, R4, R5 and R6 each represent hydrogen, then R 2 does not represent 2,4-dichlorophenyl.

In another aspect, the present invention provides a second group of novel compounds of formula I and pharmaceutically acceptable salts thereof in which: X represents oxygen; R, represents H R, represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and R3, R4, R5 and R6 are as previously defined; with the proviso that when R1, R3, R4, R5 and R6 each represent hydrogen, then R 2 does not represent phenyl, 2,4-dimethylphenyl or 2,4-dichlorophenyl.

In another aspect, the present invention provides a third group of novel compounds and pharmaceutically acceptable salts thereof in which: X represents a group of formula NR,; R, represents H;

R

2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and R3, R4, R5 and R6 are as previously defined.

In another aspect, the present invention provides a fourth group of novel compounds of formula I and pharmaceutically acceptable salts thereof in which: X represents a group of formula -C=NOR7 in which R7 represents H or a C1-4 alkyl group; RI represents H;

R

3

R

4 Rs and R. are as previously defined; WO 00/27822 PCTIS99/26105 14 with the proviso that when R1, R3, R4, R5 and R6 each represent hydrogen, and X represents C=NOH, then R2 does not represent 4-methylphenyl or 3,4-dimethoxyphenyl.

In another aspect, the present invention provides a fifth group of novel compounds of formula I and pharmaceutically acceptable salts thereof in which: X represents substituted methylene or a carbonyl group; R, represents H;

R

2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and R3, R4, R5 and R6 are as previously defined; with the provisos that 1) when X represents carbonyl or a substituted methylene, R1 is hydrogen and R3, R4, R5 and R6 each represent hydrogen, then R2 is not pyridyl, 2thienyl, 3-thienyl, phenyl or phenyl substituted by C1-2 alkyl, a halogen atom, a lower alkoxy group, a hydroxyl group or an amino group; 2) when X represents methylene, R1 is hydrogen and two of R3, R4, R5 and R6 independently represent hydrogen, halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1- 4 alkoxy or trifluoromethyl and the other two represent hydrogen, then R2 is not thienyl, furyl, pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having two or less substituents wherein the substituents are halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl; and 3) when X represents a carbonyl group, R2 represents phenyl, 4-chlorophenyl or 4-methoxyphenyl, then R3, R4, R5 and R6 are not trifluoromethyl; and 4) when X represents a carbonyl group, R2 represents phenyl, R3 represents bromo, R4 represents hydroxy-and R5 represents methoxy, then R6 is not hydrogen and WO 00/27822 PCT/US99/26105 when X represents carbonyl and R3, R4, R5 and R6 represent hydrogen, then R6 is not aryl.

In another aspect the present invention provides a sixth group of novel compounds of formula I and pharmaceutically acceptable salts thereof in which X represents a group of formula (CH2)n in which n is 1, 2 or 3 R, represents H R2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and

R

3

R

4 Rs, and R 6 are as previously defined with the proviso that when X is a group of formula (CH 2 in which n is 1, 2 or 3, R1 is hydrogen and two of R3, R4, R5 and R6 independently represent hydrogen, halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl and the other two represent hydrogen, then R2 is not thienyl, furyl, pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having two or less substituents wherein the substituents are halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl, and with the proviso that when n is 2 and R3, R4, R5 and R6 each represent hydrogen or methoxy, then R2 does not represent 3-carboxypyrid-2-yl, 3-methoxycarbonylpyrid-2-yl or 2-carboxyphenyl In another aspect the present invention provides a seventh group of novel compounds of formula I and pharmaceutically acceptable salts thereof in which X represents a) substituted methylene b) carbonyl, c) oxygen, d) a group of formula -C=NOR7 in which R7 represents H or a C1-4 alkyl group, e) a group of formula NR8 in which R8 represents H, an optionally substituted C1-4 alkyl group or optionally substituted phenyl, f) a group of formula (CH, in which n is 1, 2 or 3, or g) a group of formula S(O)p in which p is 0, 1 or 2; R, represents H;

R

2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; WO 00/27822 PCT/US99/26105 16 R3, R4 R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C,- 6 alkoxy group optionally substituted by one or more of the following: hydroxy, or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1 -6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-6 alkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-6 alkyl group a C3-8 cycloalkyl group or phenyl, wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj are as defined above, i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C 6 alkyl group, a C 1 6 alkoxy group or halo, WO 00/27822 PCT/US99/26105 17 providing that at least one of R3, R4 R5 and R 6 or a substituent on R 2 represents one of the following: a) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, b) a C1-6 alkyl group substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj are as defined above, c) a Ci.

6 alkoxy group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined above provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, d) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc wherein Rb and Rc are as defined above, e) a group of formula NRdRe in which Rd and Re are as defined above.

The term aryl as used herein means phenyl or naphthyl. The term optionally substituted as used herein means substituted by one or more of the following a) halo, b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a C, alkoxy group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, d) optionally substituted phenoxy, e) hydroxy, f) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl wherein the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: hydroxy halo a C3-12 cycloalkyl group or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom WO 00/27822 PCT/US99/26105 18 selected from O, S or N and is optionally substituted by a C1-6 alkyl group, g) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group, a phenyl C1 -6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, i) nitro, j)optionally substituted phenyl C1- 6 alkyl, k) optionally substituted phenyl C1-6 alkoxy, 1) cyano, m) a C 36 alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, r) a C1-6 alkythio group, s) phenyl, t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo.

In further embodiments, the term "optionally substituted" as used herein means substituted by one or more of the following: a) halo, b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a C.

6 alkoxy group optionally substituted by one or more of the following: hydroxy, COOH, an amino group of formula NRhRj, or an amide of the formula CONRhRR, wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo d) optionally substituted phenoxy, WO 00/27822 PCTIUS99/26105 19 e) hydroxy, f) a group of formula CORa or SORa wherein Ra is hydroxy, (CI-C 6 )alkyl,

(C,.C

6 )alkoxy or Ra represents a group of formula NRbR; where Rb and Rc independently represent hydrogen, a C 1-12 alkyl group, a C3-12 cycloalkyl group, phenyl(Co-C 6 )alkyl or heterocyclyl-(Co-C 6 )alkyl (heterocyclyl tetrahydrofuranyl, furanyl, 1,3-benzodioxole, pyridinyl, and thiophenyl) wherein the alkyl group, the cycloalkyl group, phenyl or heterocyclyl- (Co-C 6 )alkyl are optionally substituted by one or more of the following: hydroxy,

(C,-C

6 )-hyrdroxy, halo, nitro, (C,-C 6 )alkyl, (C,-C 6 )alkoxy, 6 )alkyl-hydroxy a C3-12 cycloalkyl group or an amino group of formula NRJRh; where Rh and Rj independently represent hydrogen, 2 )alkyl, (C 3

C

6 )cycloalkyl, (C 3

-C

6 )heterocycloalkyl-(Co-C 6 )alkyl (heterocycloalkyl= tetrazole, pyridine, piperidine, pyrazine, imidazole, triazole, morpoline, and piperazine), (C-C 6 )alkenyl, (C,-C)alkynyl, (C 3

-C

6 )cycloalkenyl-(C o C)alkyl, hydroxy(C-C 6 )alkyl, amino(C,-C 6 )alkyl, (C,-C 6 )alkoxy(C,- C)alkyl, mono- or di-(C,-C 6 )alkylamino(C,-C 6 )alkyl, morpholinyl-(C,- C)alkyl, pyrrolidinyl-(C,-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of halo, hydroxy, nitro, amino, mono- or di-(C,-

C

6 )alkylamino, (C,-C 6 )alkyl and (C,-C 6 )alkoxy; or Rh and Rj together with the nitrogen atom to which they are attached represent a four, five, six or seven membered heterocyclic ring which optionally contains one or more additional hetero atoms selected from O, S and N and is optionally substituted by a C 1-6 alkyl group or a heterocycle, or Rb and Rr are taken together with the nitrogen atom to which they are attached to form an optionally substituted 6- or 7-membered ring where said ring optionally contains one or more additional heteroatoms selected from the group consisting of O, N and S, and said ring is optionally substituted by (C,-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl, phenyl(C 2

-C

6 )alkenyl, where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of WO 00/27822 PCT/US99/26105 Br, Cl, F, I, hydroxy, nitro, amino, mono- or di-(C,-C 6 )alkylamino, (Ci-C 6 )alkyl and

(C,-C

6 )alkoxy; g) a group of formula NRdRe in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, S(O) 2 -phenyl, phenyl, a heterocycloalkyl-(Cl-C 6 )alkyl, wherein the heterocycloalkyl is a four, five, six or seven menbered heterocyclic ring which has one or more heteroatoms selected from the group consisting of O, S and N, or Rd and R, are each, independently,a group of formula CORf wherein Rf represents hydrogen, NRbR, (C-C,)alkoxy, amino-(C,-

C

6 )alkoxy-(C,-C,)alkoxy, mono-(C -C 6 )alkyl-amino-(C,-C 6 )alkoxy-(C -C 6 )alkoxy, N,N-di-(Cl-C 6 )alkyl-amino-(C,-C 6 )alkoxy-(C,-C 6 )alkoxy, a C1-12 alkyl group, a C3- 12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkoxy, the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro, (C,-C 6 )alkyl, (C 1

-C

6 )alkoxy, di-(C-C 6 )alkyl-amino-(C-C 6 )alkoxy, pyrrolidine which is optionally substituted with a (C,-C 6 )alkyl, or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or i) nitro, j) optionally substituted phenyl C1-6 alkyl, k) optionally substituted phenyl C1-6 alkoxy 1) cyano, m) a C 3 6 alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring-is optionally substituted by one or more of the following trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, WO 00/27822 PCT/US99/26105 21 r) a C1-6 alkythio group s) phenyl t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, u) CHO, v) dihydroxyborane w) tetrazolyl.

When R2 represents optionally substituted pyridyl the pyridine ring may be in the form of its N-oxide.

When NRdRe represents a saturated heterocyclic ring the ring is preferably morpholino, perhydrothiazin-4-yl, piperidino, pyrrolidin-1-yl, piperazin-1-yl or 4methylpiperazin-4-yl.

The term substituted methylene means for example methylene substituted by one or more of the following: hydroxy or a C1-4 alkyl group wherein the alkyl group is optionally further substituted by a group of formula NRrRs wherein Rr and Rs independently represent H or a C1-6 alkyl group.

It will be understood that any group mentioned herein which contains a chain of three or more atoms signifies a group in which the chain may be straight or branched. For example, an alkyl group may comprise propyl, which includes n-propyl and isopropyl, and butyl, which includes n-butyl, sec-butyl, isobutyl and tert-butyl.

The term C3-12 cycloalkyl group includes bridged groups for example adamantyl.

The term 'halo' as used herein signifies fluoro, chloro, bromo and iodo.

Compounds of formula I may exist as salts with pharmaceutically acceptable acids. The present invention includes such salts. Examples of such salts include hydrochlorides, hydrobromides, sulphates, methanesulphonates, nitrates, maleates, acetates, citrates, fumarates, tartrates [eg (+)-tartrates, (-)-tartrates or mixtures thereof including racemic mixtures], succinates, benzoates and salts with amino acids such as glutamic acid. These salts may be prepared by methods known to those skilled in the art.

WO 00/27822 PCT/US99/26105 22 Certain compounds of formula I which have acidic substituents may exist as salts with pharmaceutically acceptable bases. The present invention includes such salts. Example of such salts include sodium salts, potassium salts, lysine salts and arginine salts. These salts may be prepared by methods known to those skilled in the art.

Certain compounds of formula I and their salts may exist in more than one crystal form and the present invention includes each crystal form and mixtures thereof.

Certain compounds of formula I and their salts may also exist in the form of solvates, for example hydrates, and the present invention includes each solvate and mixtures thereof.

Certain compounds of formula I may contain one or more chiral centres, and exist in different optically active forms. When compounds of formula I contain one chiral centre, the compounds exist in two enantiomeric forms and the present invention includes both enantiomers and mixtures of enantiomers. The enantiomers may be resolved by methods known to those skilled in the art, for example by formation of diastereoisomeric salts which may be separated, for example, by crystallization; formation of diastereoisomeric derivatives or complexes which may be separated, for example, by crystallization, gas-liquid or liquid chromatography; selective reaction of one enantiomer with an enantiomer-specific reagent, for example enzymatic esterification; or gas-liquid or liquid chromatography in a chiral environment, for example on a chiral support for example silica with a bound chiral ligand or in the presence of a chiral solvent. It will be appreciated that where the desired enantiomer is converted into another chemical entity by one of the separation procedures described above, a further step is required to liberate the desired enantiomeric form.

Alternatively, specific enantiomers may be synthesized by asymmetric synthesis using optically active reagents, substrates, catalysts or solvents, or by converting one enantiomer into the other by asymmetric transformation.

When a compound of formula I contains more than one chiral centre it may exist in diastereoisomeric forms. The diastereoisomeric pairs may be separated by methods known to those skilled in the art, for example chromatography or crystallization and the individual enantiomers within each pair may be separated as WO 00/27822 PCT/US99/26105 23 described above. The present invention includes each diastereoisomer of compounds of formula I and mixtures thereof.

Certain compounds of formula I may exist in different tautomeric forms or as different geometric isomers, and the present invention includes each tautomer and/or geometric isomer of compounds of formula I and mixtures thereof.

Certain compounds of formula I may exist in different stable conformational forms which may be separable. Torsional asymmetry due to restricted rotation about an asymmetric single bond, for example because of steric hindrance or ring strain, may permit separation of different conformers. The present invention includes each conformational isomer of compounds of formula I and mixtures thereof.

Certain compounds of formula I may exist in zwitterionic form and the present invention includes each zwitterionic form of compounds of formula I and mixtures thereof.

The compounds of this invention are useful as inhibitors of serine/ threonine and tyrosine kinases. In particular, the compounds of this invention are useful as inhibitors of tyrosine kinases that are important in hyperproliferative diseases, especially in the process of angiogenesis. Since these compounds are antiangiogenic, they are important substances for inhibiting the progression of disease states where angiogenesis is an important component.

Preferred definitions of the substituents are now given.

Preferably R, represents optionally substituted phenyl, naphthyl, optionally substituted thienyl, optionally substituted pyridyl, optionally substituted furyl, or optionally substituted pyrrolyl.

More preferably R 2 represents 2-thienyl, 3-thienyl, 2-furyl, 3-furyl, 2-pyridyl, 3-pyridyl or 4-pyridyl each of which is optionally substituted, naphthyl and optionally mono-, di- and tri-substituted phenyl, wherein the substituents are selected from optionally substituted alkoxy (particularly methoxy, 3morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxymethoxy, 2carboxyethoxy, 2-carbamoylethoxy, carbamoylmethoxy, 3-carbamoylpropoxy, 2piperidinoethoxy, 2-(piperazin-l-yl)ethoxy, 2-(pyrrolidin-l-yl)ethoxy, 2dimethylaminoethoxy, 2-(perhydro-thiazin-4-yl)ethoxy, 3-piperidinopropoxy, 3- (piperazin-1-yl)propoxy, 3-(pyrrolidin-lyl)-propoxy, 3-dimethylaminopropoxy, 3- WO 00/27822 PCTIUS99/26 105 24 (perhydrothiazin-4-yl)propoxy), lower alkyl (particularly methyl), halo (particularly fluoro and chioro), aryl (particularly phenyl), hydroxy, aryloxy (particularly phenoxy), arylalkoxy (particularly benzyloxy), di-lower-alkylamino (particularly dimethylamino), polyhalo-lower-alkyl, polyhalo-lower-alkoxy (particularly difluoromethoxy), nitro, cyano, lower-alkylthio (particularly methylthio), carboxy, lower-alkoxycarbonyl (particularly methoxycarbonyl), amido (particularly acetamido and benzamido) and optionally substituted carbamoyl (particularly carbamoyl, N-methylcarbamoyl, N-phenylcarbamoyl) and a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro.

Most preferably R 2 represents 4-pyridyl, 2-fonmamidomethyl-4-pyridyl, 2aminomethyl-4-pyridyl, 2-(hydroxyamidino)-4-pyridyl, 2-carbamoyl-4-pyridyl, 4-pyridyl-N-oxide, 2-chloro-4-pyridyl, 2-cyano-4-pyridyl, 5-methyl-2-furyl, 5-(2nitro-4-trifluoromethylphenyl)fiir-2-yl, phenyl, 4-methoxyphenyl, 3-methoxyphenyl, 2-methoxyphenyl, 3 ,4-dimethoxyphenyl, 3,4,5-trimethoxyphenyl, 4-(3-morpholinopropoxy)phenyl, 4-(2-morpholinoethoxy)phenyl, 4-(3-carboxypropoxy)phenyl, 4carboxymethoxyphenyl, 4-(3 -carbamoylpropoxy)phenyl, 4carbamnoylmethoxyphenyl, 3-(3-morpholino-propoxy)phenyl, 3-(2morpholinoethoxy)phenyl, 3-(3-carboxy-propoxy)phenyl, 4-carboxymethoxyphenyl, 3-(3 -carbamoylpropoxy)phenyl, 3-carbamoylmethoxyphenyl, 2-hydroxyphenyl, 3hydroxyphenyl, 4-hydroxyphenyl, 3 -hydroxy-4-methoxyphenyl, 4-hydroxy-3methoxyphenyl, 4-difluoromethoxyphenyl, 3-nitrophenyl, 4-nitrophenyl, 3 butyl-4-hydroxyphenyl, 4-methyiphenyl, 4-fluorophenyl, 2-chiorophenyl, 3chiorophenyl, 4-chlorophenyl, 2,4-dichlorophenyl, 2-chloro-5-nitrophenyl, 4-fluoro- 2-chlorophenyl, 4-methylthiophenyl, 4-biphenylyl, 3-phenoxyphenyl, 4phenoxyphenyl, 4-benzyloxyphenyl, 4-dimethylaminophenyl, 4diethylaminophenyl, 4-methoxycarbonylphenyl, 4-carbamoylphenyl, 4-cyanophenyl, 4-N-methylcarbamoylphenyl, 4-N-phenylcarbamoylphenyl, 4-acetamidophenyl, 4benzamidophenyl 4-carboxyphenyl, 4 2 -diethylaminoethyl)carbamoyl]phenyl, 4-(prop-l1-enyloxy)phenyl, 3-(2-hydroxyethoxy)phenyl, 3-(N-(2-diethyl aminoethyl)carbamnoylmethoxy)phenyl, 3 3 -(N-(2-diethylaminoethyl)carbamoyl) propoxy]phenyl, 4 2 -diethylaminoethyl)carbamoylmethoxy)phenyl, WO 00/27822 PTU9/60 PCT/US99/26105 diethylaminoethyl)-carbamoyl)propoxy]phenyl, 2-furyl, 5-[3,5bis(trifluoromethyl)phenyl]-2-fiiryl, 3 -bromo-2-thienyl, 5 -methoxy-2-furyl, 5 nitro-4-trifluoromethylphenyl)-2-furyl, 3 -N-(2-morpholinoethyl) carbamoylmethoxy)phenyl, 3-[3 -(N-(2-morpholinoethyl) carbamoyl)propoxyphenyl], 4-(N-(2-morpholinoethyl)-carbamnoylmethoxy)phenyl 4- (morpholinoacetamido)phenyl and 4-[3-(N-(2-morpholinoethyl)carbamoyl)propoxy]phenyl.

Preferably R 3 R, and R 6 independently represent hydrogen halo (particularly fluoro), optionally substituted lower alkoxy (particularly methoxy, 3morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxymethoxy, 2carboxyethoxy, 2-carbamoylethoxy, 3-carbamoylpropoxy, 2-piperidinoethoxy, 2- (pip erazin- 1 -yl)ethoxy, 2-(pyrrolidin- 1-yl)ethoxy, 2-dimethylaminoethoxy, 2- (perhydrothiazin- 1-yl)ethoxy, 3-piperidinopropoxy, 3 -(piperazin- 1-yl)propoxy, 3- (pyrrolidin- 1-yl)propoxy, 3-dimethylaminopropoxy, 3-(perhydrothiazin-4yl)propoxy), carbamnoylmethoxy, hydroxypropyloxy, hydroxyethoxy, (3morpholino)propoxy and 2-morpholino)ethoxy), amido (particularly acetamido and benzamido), optionally substituted carbamnoyl (particularly carbamoyl, N-methylcarbamoyl and N-phenylcarbamoyl), carboxy, nitro and amino.

More preferably R 3

R

4 R, and R, represent the following 6,7-dimethoxy, 6,7,8-trimethoxy, 6-fluoro, 6-acetamido, 7-methoxy, 6-carbamoyl, 6-(N-methylcarbamoyl), 6-(N-phenylcarbamoyl), (3-morpholino)propoxy and 2-morpholino)ethoxy.

The term lower as used herein means a group having 1 to 6 carbon atoms.

Specific compounds of the present invention include: 3-(3 ,4,5-trimethoxyphenyl)- 1 ,4-dihydroindeno[ [1,2-c]pyrazole, 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenol, 3-phenyl- IH-[ 1 ]benzothieno[3,2-c]pyrazole, 3-(2-thienyl)- 1H-[ 1 ]benzothieno[3 ,2-c]pyrazole, 3-phenyl- 1 1 ]benzothieno[3 ,2-c]pyrazol e 4-oxide, 3-phenyl- 1H-[l1]benzothieno[3,2-c]pyrazole 4,4-dioxide, 3-(2-thienyl)-l1H-[ 1]benzothieno[3,2-c]pyrazole, 3-phenylindeno[1 ,2-c]pyrazol-4(1R)-one oxime, WO 00/27822 WO 0027822PCT[US99/26105 26 3 ,4-dimethoxyphenyl)indeno[1I,2-c]pyrazol-4( IH1)-one oxime, 3-(4-methylphenyl)indeno[ 1,2-cjpyrazol-4( IH1)-one oxime, 3 -(2-thienyl)indeno[ 1,2-c]pyrazol-4( 1H)-one, 3-phenyl- 1H-benzofuro[3,2-c]pyrazole, 1 ,4-dihydro-3-phenylpyrazolo[4,3-b]indole, 1 ,4-dihydro-4-methyl-3-phenylpyrazolo[4,3-b]indole, 4,4-dimethyl-3 -phenyl- 1,4-dihydroindeno [1 ,2-c]pyrazole, 1,4-dihydroindeno[l1,2-c]pyrazol-3-yl)benzoic acid, methyl 4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzoate, 1,4-dihydroindeno[ 1, 2 -c]pyrazol-3-yl)acetanilide, 1,4-dihydroindeno[l1,2-c]pyrazol-3 -yl)-3 -morpholinopropionanilide, 1,4-dihydroindeno[ 1, 2 -c]pyrazol-3-yl)morpholinoacetanilide, 4' ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzanilide, N-(3-phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)acetamide, 3-morpholino-N-(3-phenyl- 1,4-dihydroindeno[ 1, 2 -clpyrazol-6-yl)propionamide, N-(3-phenyl- 1,4-dihydroindeno[1I,2-c]pyrazol-6-yI)benzanilide, 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-methyl-4-( 1,4-dihydroindeno[ 1,2-c~pyrazol-3-yl)benzamide, 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzanilide, N -(2-diethylaminoethyl)-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamnide, N-(2-morpholinoethyl)-4-(1 ,4-dihydroindeno[1 2 -c]pyrazol-3-yI)benzamide, 4-(1 ,4-dihydroindeno[1 ,2-c]pyrazol-3-yl)phenol, 3-[3-(2-morpholinoethoxy)phenyl]-1I,4-dihydroindeno [1 ,2-c]pyrazole, 3-(2-thienyl)-1 ,4-dihydroindeno[1,2-c]pyrazol-6-ol, 6-(2-morpholinoethoxy)-3-(2-thienyl) 1 ,4-dihydroindeno[ 1,2-c]pyrazole, 3 -[3-(2-hydroxyethoxy)phenyl]- 1,4-dihydroindeno[ 1,2-cjpyrazole, 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenoxyacetic acid, ethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenoxyacetate, 3-(1 ,4-dihydroindeno[1 2 -c]pyrazol-3-yl)phenoxyacetamide, N-(2-diethylamninoethyl)-3-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)phenoxyacetamide, N-(2-morpholinoethyl)-3-( 1,4-dihydroindeno[ 1, 2 -c]pyrazol-3-yl)phenoxyacetamide, WO 00/27822 PTU9/60 PCT/US99/26105 27 4- {3-(1I,4-dihydroindeno[ I,2-c]pyrazol-3-yl)phenoxy} butyric acid, ethyl 4- 1,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenoxy) butyrate, 4- 1,4-dihydroindeno [1 ,2-c]pyrazol-3-yl)phenoxy} butyramide, N-(2-diethylaminoethyl)-4- {3 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenoxy} butyramide, N-(2-morpholinoethyl)-4- 1,4-dihydroindeno [1 ,2-c]pyrazol-3-yl)phenoxy} butyramide, 3-(2-thienyl)-1I,4-dihydroindeno[ 1,2-c]pyrazole-6-carboxamnide, N-methyl-3-(2-thienyl)- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole-6-carboxamide, N-(2-rnorpholinoethyl)-3-phenyl- 1 ,4-dihydroindeno[ [1,2-clpyrazole-6-carboxamide, 3 -(2-thienyl)- 1 ,4-dihydroindeno[ I ,2-c]pyrazole-6-carboxanilide; N-(3-phenyl- 1 ,4-dihydroindeno[ 1 ,2-c~pyrazol-6-yI)acetamnide, 3-phenyl-1 ,4-dihydroindeno[1 ,2-c]pyrazol-6-ylamine, 3-(4-nitrophenyl)- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole, 4-(1I,4-dihydroindeno[ I ,2-c]pyrazol-3 -yl) aniline, 4-(4,5-dihydro- 1H-benzo[g]indazol-3 -yl)pyridine 1-oxide, 3-(2-chloro-4-pyridyl)-4,5-dihydro- 1H-benzo[g]indazole, 4-(4,5-dihydro- 1H-benzo[g]indazol-3-yl)-2-pyridinecarbonitrile, 4-(4,5-dihydro- 1H-benzo[g]indazol-3-yl)-2-pyridinecarboxainide oxime, 4-(4,5-dihydro- 1H-benzo[g]indazol-3-yl)-2-pyridinecarboxamide, [4-(4,5-dihydro- 1H-benzo[g~indazol-3-yl)-2-pyridyl]methyl} ammonium chloride, N- {[4-(4,5-dihydro- IH-benzo[g]-indazol-3-yl)-2-pyridyl]methyl} formnamide, 1,4-dihydroindeno[ 1 ,2-c~pyrazol-3-yl)phenoxy] ethanol, 2-morpholinoethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)benzoate, 3-(3-nitrophenyl)- 1,4-dihydroindeno[ 1,2-cjpyrazole, 3-(4-thiomethoxyphenyl)-l1,4-dihydroindeno[ 1,2-c]pyrazole, 3-(2-naphthyl)- 1,4-dihydroindeno[1I,2-c]pyrazole, 3-(4-difluoromethoxyphenyl)- 1,4-dihydroindeno[ 1,2-clpyrazole, 3 -(4-acetamnidophenyl)-4,5-dihydro-2H-benz[g]indazole, 3-(4-bromo-2-thienyl)-4,S-dihydroindeno[1I,2-c]pyrazole, 3 -(4-benzyloxyphenyl)-4,5-dihydro-2H-benzg]indazole, 6,7-dimethoxy-3-(3-phenoxyphenyl)- 1 ,4-dihydroindeno-[ 1 ,2-c]pyrazole, WO 00/27822PC/S9610 PCTIUS99/26105 28 -trifluoromethyl-2-pyridyloxy)phenyl]. 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole, 6,7,8-trimethoxy-3-(2,3 ,4-trimethoxyphenyl)- I ,4-dihydroindeno[ 1 ,2-c]pyrazole, 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)-2-hydroxymnethyl)phenol, 1,4-dihydroindeno[ [1,2-c]pyrazol-3 -yI)phenol, 2-chloro-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenol, 2-methoxy-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenol, 3-chloro-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenol, 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenoxy]acetamide, 4 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)diethylamino-acetanilide, 4-(l1I-[ 1 lbenzothi eno[3 ,2-c]pyrazol-3-yl)benzamide, 3 -(4-aminophenyl)- lH-[ 1]benzothieno [3 ,2-c]pyrazole, 3-(4-methoxyphenyl)- 1H-benzothieno[3 ,2-c]pyrazole, 3-(4-hydroxyphenyl)- 1H-[ I]benzothieno[3 ,2-c]pyrazole, N-(3-phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzamide, N-(2-morpholinoethyl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, 4-(1,4-dihydroindeno[1 ,2-c]pyrazol-3-yl)benzonitrile, 7 -methoxy-3-(4-methylsulphonylpheny1)-4,5-dihydro-2H-benz[gindazole, 4-methyl-3-phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-4-ol, N-[2-(N,N-diethylamino)ethyl]-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)benzamnide, N-[2-(N,N-dimethylamino)ethyl]-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)benzamnide, N-[2-(N,N-dipropylamino)ethyl]-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)benzamide, N-[2-(N,N-di-isopropylamino)ethyl]-4-( 1,4-dihydroindeno[l1,2-c]pyrazol-3yl)benzamide, N-[3 -(N,N-diethylamino)propyl]-4-( 1,4-dihydroindeno[1I,2-cjpyrazol-3yl)benzamide, N-[3-(N,N-dimethylamino)propyl]-4-( 1,4-dihydroindeno [1 ,2-clpyrazol-3 yl)benzamide, N-[3-(N,N-dipropylamino)propyl]-4-(1 ,4-dihydroindeno[ 1 ,2-c~pyrazol-3yl)benzamide, WO 00/27822 WO 0027822PCTIUS99/261 29 N-[3-(N,N-di-isopropylamino)propyl]-4-( 1,4-dihydroindeno 1,2-c]pyrazol-3yl)benzamide, N-(2-Piperidinoethyl)-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamide, N-(2-Piperidinopropyl)-4-( 1,4-dihydroindeno[ [1,2-c]pyrazol-3-yl)benzamide, N-(2-Morpholinoethyl)-4-(1I,4-dihyclroindeno[ 1,2-c]pyrazol-3-y1)benzamide, N-(2-Morpholinopropyl)-4-(1 ,4-dihydroindeno[1 ,2-c]pyrazol-3-yl)benzamide, N-[2-(Piperazin- 1 -yl)ethyl]-4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)benzamide, N-[3-(Piperazin- 1 -yl)propyl]-4-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamide, N- [2-(Pyrrolidin- 1 -yl)ethyl]-4-( 1,4-dihydroindeno[ [1,2-c~pyrazol-3-yl)benzamide, N-[3-(Pyrrolidin- I -yl)propyl]-4-( 1,4-dihydroirxdeno[ [1,2-c]pyrazol-3-yl)benzamide, N-[2-(4-Methylpiperazin- 1 -yl)ethyl]-4-( 1,4-dihydroindeno[ [1,2-c]pyrazol-3yl)benzamide, N-[3-(4-Methylpiperazin- I -yl)propyl]-4-( 1,4-dihydroindeno[ 1 ,2-clpyrazol-3yl)benzamide, N-[2-(Thiomorpholin- I -yl)ethyl]-4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3 yl)benzainide, N-[3-(Thiomorpholin- 1-yl)propyl]-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)benzamide, N-[2-(Homopiperazin-1I-yl)ethyl]-4-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3yl)benzamide, N-[3-(Homopiperazin- I -yl)propyl]-4-(1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)benzamide, N-[2-(Perhydroazepin- 1 -yl)ethyl]-4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3yl)benzamide, N-[3-(Perhydroazepin- 1 -yl)propyl]-4-( 1,4-dihydroindeno[ 1 ,2-clpyrazol-3yl)benzamide, N-Isopropyl-4-( 1,4-dihydroindeno[ 1,2-clpyrazol-3-yl)benzamide, N-But-2-yl-4-(l1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamnide, N-Methyl-4-( 1,4-dihydroindeno[1I,2-c]pyrazol-3-yl)benzamide, N-Ethyl-4-( 1,4-dihydroindeno[ 1,2-cjpyrazol-3-yl)benzamide, N-Pentyl-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-(2-Bromoethyl)-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, WO 00/27822 WO 0027822PCT/LJS99/261 N-(3 ,3 ,3-trifluoroprop-I -yl-4-(l ,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamide, N-(Cyclopropylmethyl)-4-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamnide, N-Cyclopentyl-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamide, N-(Cyclohexylmethyl)-4-( 1,4-dihydroindeno [1 ,2-c~pyrazol-3 -yl)benzamide, N-(2-Chlorocyclopentyl)-4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)benzamide, N-[3-(N,N-dimethylamino)-2,2-dimethylpropyl]-4-( 1,4-dihydroindeno[ 1,2c]pyrazol-3 -yl)benzamide, N-[3 -(2-Methylpiperi din- 1 -yl)propyl]-4-(1 ,4-dihydroindeno[ [1,2-c]pyrazol-3yl)benzamide, N-(3-Methylbut-2-yl)-4-(1 ,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)benzaniide, N-[2-(Pyrrolidin- 1 -yl)ethyl]-4-(1I,4-dihydroindeno[ [1,2-c]pyrazol-3-yl)benzamide, N-[3-(N,N-Dimethylamino)prop-2-yl]-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)benzamide, N-(2-Hexyl)-4-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-tert-Butyl-4-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-[7-(N,N-Dimethylainino)heptyl]-4-( 1,4-dihydroindeno[ [1,2-c]pyrazol-3 yl)benzamide, N-(2-Methylbut-2-yl)-4-( 1,4-dihydroindeno[ 1 ,2-c~pyrazol-3-yl)benzamide, N-(2-Pentyl)-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)benzamide, N-sec-Butyl-4-( 1,4-dihydroindeno[ 1 ,2-c~pyrazol-3 -yl)benzamide, N-(3 ,3-Dimethylbutyl)-4-( 1,4-dihydroindeno [1 ,2-c]pyrazol-3-yl)benzamide, N-(2,2,3,3 ,3-Pentafluoropropyl)-4-(1I,4-dihydroindeno[ 1,2-clpyrazol-3yl)benzamide, N-(2,5-Dichloropentyl)-4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)benzamide, N-(2,2-Difluoroethyl)-4-( 1,4-dihydroirideno[ 1 ,2-c~pyrazol-3-yl)benzamide, N-(2-Chloroethyl)-4-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamnide, N-[2-(N,N-dimethylamino)propyl]-4-( 1,4-dihydroindeno[ [1,2-c]pyrazol-3yl)benzamide, N-(3-Morpholinopropyl)-4-( 1,4-dihydroindeno[1I,2-c]pyrazol-3-yl)benzaxnide, N-[3-(Pyrrolidin- 1 -yl)propyl]-4-( 1,4-dihydroindeno[ [1,2-c]pyrazol-3-yl)benzamide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)piperidinoacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-4-methylpiperazin- 1-ylacetanilide, WO 00/27822PCUS9210 PCT/US99/26105 31 (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)-4-methylhomopiperazin- 1-ylacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)piperazin- 1-ylacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yI)homopiperazin- 1-ylacetanilide, (1 ,4-Dihydroindeno[ 1, 2 -clpyrazol-3-yl)dipropylaminoacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)dimethylaminoacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)fluoroacetanilide, (1 ,4-Dihydroindeno[ 1,2-clpyrazol-3 -yl)-3 (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-4-fluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-2-fluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-3-fluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)-2,4-difluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-2,3 -difluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-4-nitrobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)-3-nitrobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-3 ,3,3-trifluoropropananilide, (1 ,4-Dihydroindeno[1 ,2-c]pyrazol-3-yl)isobutananilide, (1 ,4-Dihydroindeno[1I,2-c]pyrazol-3-yl)isopentananilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)-2-methylbutananilide, (1 ,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)-2-rnethylpentananilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-2-ethylbutananilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)neopentylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-4,4-dimethylpentananilide, (1 ,4-Dihydroindeno[1 ,2-c]pyrazol-3-yl)cyclohexananilide, (1 ,4-Dihydroindeno[ 1, 2 -clpyrazol-3-yl)trifluoroacetanilide, (1 ,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)pentafluoropropananilide, Fluoro-N-(3-Phenyl-1,4-dihydroindeno[ 1, 2 -c]pyrazol-6-yl)acetamide, 3,5-Difluoro-N-(3-Phenyl-l1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamide, 4-Fluoro-N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamide, 2-Fluoro-N-(3-Phenyl- 1,4-dihydroindeno [1 ,2-c]pyrazol-6-yl)benzylamide, 3-Fluoro-N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamide, 2,4-Difluoro-N-(3-Phenyl- 1,4-dihydroindeno [1 ,2-c]pyrazol-6-yl)benzylamjde, WO 00/27822 WO 0027822PCT/1US99/26105 32 2,3 -Difluoro-N-(3-Phenyli 1,4-dihydroindeno[ I 2 -c]pyrazol-6-yl)benzylamide, -Difluoro-N-(3 -Phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)benzylamnide, 4-Nitro-N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)benzylamide, 3-Nitro-N-(3-Phenyl- 1 ,4-dihydroindeno 1,2-c]pyrazol-6-yl)benzylamide, 3 ,3,3-Trifluoro-N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)propanaxnide, N-(3-Phenyl- I ,4-dihydroindeno 1,2-c]pyrazol-6-yl)isobutanamide, N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-clpyrazol-6-yl)isopentanamide, 2-Methyl-N-(3 -phenyl- 1 ,4-dihydroinderio[ 1 ,2-c]pyrazol-6-yI)butanamnide, 2-Methyl-N-(3 -phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)pentanamide, 2-Ethyl-N-(3-phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)butanamide, N-(3-Phenyl- I ,4-dihydroindeno[ 1 2 -c]pyrazol-6-yl)neopentanamide, 4,4-Dimethyl-N-(3-phenyl-1I,4-dihydroindeno[1 ,2-c]pyrazol-6-yl)pentanamide, N-(3-Phenyl- I ,4-dihydroindeno[ 1 2 -c]pyrazol-6-yl)cyclohexanecarboxamide, Trifluoro-N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)acetamide, Pentafluoro-N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)propanamide, (1 ,4-Dihydroindeno [1 ,2-c]pyrazol-3-yl)acetanilide, 2-Hydroxy-N-(2-morphoinoethy)-5-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)benzamide, 2-Hydroxy-N-(2-morpholinopropyl)-4-(1 ,4-dihydroindeno[ [1,2-c]pyrazol-3yl)benzamide, 2-Hydroxy-N-[2-(pyrrolidin-i1 -yl)ethyl]-5-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3 y1)benzamide, 2-Hydroxy-N-[3-(pyrrolidin- I -yl)propyl]-5-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)benzamide, N-[2-(N,N-Diethylamino)ethyl]5-(1,4-dihydroindeno[ [1,2-c]pyrazol-3-yl)-2hydroxybenzamide, 1,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)-2hydroxybenzamide, 2 -Hydroxy-N-[2-(N,N-dimethylamino)ethyl]s.( 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3 yl)benzaxnide, 2-Hydroxy-N-[3-(N,N-dimethylamino)propyl]ps(1 ,4-dihydroindeno[1 ,2-c]pyrazol- 3-yl)benzamide, WO 00/27822 WO 0027822PCTJUS99/26105 33 2-Hydroxy-N-[2-(N,N-dipropylamino)ethyl]-5 ,4-dihydroindeno [1 ,2-c]pyrazol-3yl)benzamnide, 2-Hydroxy-N- [2-(N,N-di-isopropylamino)ethyl]-5-(1I,4-dihydroindeno[ 1,2c]pyrazol-3-yl)benzamide, 2-Hydroxy-N-[3-(N,N-dipropylamino)propyl]-5-( 1,4-dihydroindeno[1I,2-c]pyrazol- 3 -yl)benzamide, 2-Hydroxy-N-[3-(N,N-di-isopropylamino)propyll-5-( 1,4-dihydroindeno[ 1,2c~pyrazol-3-yl)benzamide, 2-Hydroxy-N-(2-piperidinoethyl)-5 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)benzamide, 2-Hydroxy-N-(2-piperidinopropyl)-5-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)benzamide, 2-Hydroxy-N-[2-(piperazin- 1 -yl)ethyl]-5-(1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)benzamide, 2-Hydroxy-N-[3-(piperazin- 1 -yl)propyl]-5-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3yl)benzamide, 2-Hydroxy-N-[2-{4-methylpiperazin- I -yl)ethyl]-5-(1 ,4-dihydroindeno[ 1,2c]pyrazol-3-yl)benzamide, 2-Hydroxy-N-[3-(4-methylpiperazin- I -yl)propyl]-5-(1 ,4-dihydroindeno[1 ,2c]pyrazol-3-yl)benzamide, 2-Hydroxy-N-[2-(thiomorpholin- 1-yl)ethyl]-5 ,4-dihydroindeno[ 1,2-c]pyrazol-3yl)benzamide, 2-Hydroxy-N-[3-(thiomorpholin- 1 -yl)propylll-5-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)benzamnide, 2-Hydroxy-N-[2-(Homopiperazin- 1 -yl)ethyl]-5-(1 ,4-dihydroindeno[ 1 ,2-c~pyrazo1-3yl)benzamide, 2-Hydroxy-N-[3-(Homopiperazin- 1 -yl)propyl]-5-( 1,4-dihydroindeno[ 1,2-c]pyrazol- 3-yl)benzamide, 2-Hydroxy-N-[2-(Perhydroazepin- I -yl)ethyl]-5-( 1,4-dihydroindeno [1 ,2-c]pyrazol-3yl)benzamide, 2-Hydroxy-N-[3-(Perhydroazepin- 1 -yl)propyl]-5-( 1,4-dihydroindeno[ 1,2-c]pyrazol- 3-yl)benzamide, WO 00/27822 WO 0027822PCT/US99/26105 34 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-N-(2-morphilinoethyl)alilifle, 4-(1 ,4-Dihydroindeno[ 1 ,2-c]pyrazo1-3-y1)-N-(2-morphiinopropy1)alinfe, 1,4-Dihydroindeno[ 1 ,2-clpyrazol-3 -yl)-N-(2-piperidinoethyl)aniline, 1,4-Dihydroindeno[ [1,2-c]pyrazol-3 -yl)-N-(2-piperidinopropyl)aniline, 1,4-Dihydroindeno[ [1,2-c]pyrazol-3-yl)-N-[2-(thiomorphilifl -yl)ethyl] aniline, 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)-N-112-(thiomorphilifl 1 -yl)propyl] aniline, 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)-N-[2-(piperazin- 1 -yl)ethyl] aniline, 1,4-Dihydroindeno[ 1 ,2-c]pyrazol..3-yl)-N-[I2-(piperazin- 1 -yl)propyi] aniline, 1,4-Dihydroindeno[ 1 ,2-cjpyrazol-3 -yl)-N-[2-(4-methylpiperazin- 1yl)ethyl] aniline, 1,4-Dihydroindeno[ [1,2-c]pyrazol-3 -yl)-N-[2-(4-methylpiperazin- 1 yl)propyl] aniline, N-[2-(N,N-Diethylamino)ethyll-4-(1I,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)aniline, N-[3-(N,N-Diethylamino)propyl]-4-( 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-y1)aniline, 1,4-Dihydroindeno[ 1 ,2-clpyrazo1-3-y1)-N-[2-(N,N-dipropyamilo)ethy1] aniline, 4-(1 ,4-Dihydroindeno[ 1 ,2-c]pyrazo1-3-y1)-N-II3-(N,N-dipropyamio)propy1] alinle, 4-(1 ,4-Dihydroindeno[ 1,2-c]pyrazo1-3-yl)-N-!I2-(N,N-dimethy1amilo)ethy1]alinife, 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)-N- [3 -(N,N-dimethylamilo)propyl] anilinle, Methyl 4-(6-Acetamido- 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)benzoate, N-(3-Methoxypropyl)-4-( 1,4-dihydroindeno[ 1,2-c~pyrazol-3-yl)benzamide, 1,4-Dihydroindeno[1I,2-clpyrazol-3-yl)-N-(4-Nitrophenyl)belzamide, N-(3-Phenyl-1I,4-dihydroindeno[ 1,2-c]pyrazolb6-yl)morpholinoacetamide, N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)morpholinoacetamide, N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)piperidinoacetamide, N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-clpyrazol-6-yl)thiomorpholinoacetamide, N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)-4-methylpiperazin- 1 ylacetamide, N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c~pyrazo-6-y1)piperazifl-1I-ylacetamide, N-(3-Phenyl- I ,4-dihydroindeno[ 1 ,2-cI~pyrazol-6-y1)pyrrolidin- 1 -ylacetamide, 2-(N,N-Diethylaniino)-N-(3-phenyl-1,4-dihydroindelo[1 ,2-c~pyrazol-6yl)acetamide, N-(3 -Phenyl-1I,4-dihydroindeno[ 1,2cprzl6y 2(imtyaioaeaie WO 00/27822PC/S/610 PCT/US99/26105 N-(3 -Phenyl- 1 ,4-dihydroindeno[ [1, 2 -c]pyrazol-6-yl)-2-(dipropylamino)acetamide, 4-(6-Amino-1I,4-dihydroindeno[l1,2-c]pyrazol-3 -yl)-N-i12-(N,N-diethylamino)ethyl] benzamnide, 3 -[3-(2-Morpholinoethoxy)phenyl]- 1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3-[3-(2-Morpholinoethoxy)phenyl]- 1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3- [3-(3-Morpholinopropoxy)phenyl]- 1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3-[3-(2-Piperidinoethoxy)phenyl]- 1 ,4-dihydroindeno[ [1,2c]pyrazole, 3 -13-(3-Piperidinopropoxy)phenyl]- 1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3- f{3-[2-(Piperazin- I -yI)ethoxy]phenyll -1,4-dihydroindeno[ 1,2c]pyrazole, 3- 3-[3-(Piperazin- I -yl)propoxy]phenyl} 1,4-dihydroindeno[ 1 ,2c]pyrazole, 3- {3-[2-(4-Methylpiperazin- 1 -yI)ethoxy]phenyl}- -1,4-dihydroindeno[ 1,2c]pyrazole, 3- {3-[3-(4-Methylpiperazin- 1 -yl)propoxy]phenyl} 1,4-dihydroindeno[ 1 ,2c]pyrazole, 3- 3-[2-(Homopiperazin- 1 -yl)ethoxy]phenyl} -1 ,4-dihydroindeno[ 1,2c]pyrazole, 3- {3-[3-(H-omopiperazin- 1 -yl)propoxy]phenyl} -1 ,4-dihydroindeno[1 ,2c]pyrazole, 3- {3-[2-(4-Methylhomopiperazin- 1 -yl)ethoxy]phenyl -1,4dihydroindeno[ 1 ,2c]pyrazole, 3- {3-[3-(4-Methylhomopiperazin- I -yl)propoxy]phenyl} 1,4dihydroindeno[ 1 ,2c]pyrazole, 3- {3-[2-(N,N-Diethylamino)ethoxy]phenyl} -1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3- {3-[3-(N,N-Diethylamino)propoxy]phenyl} 1,4-dihydroindeno[ 1 ,2c]pyrazole, 3- 3-[2-(N,N-Dimethylamino)ethoxy]phenyl} 1,4-dihydroindeno[ 1 ,2c]pyrazole, 3- {3-13-(N,N-Dimethylamino)propoxy]phenyl} -1 ,4-dihydroindeno[l1,2c]pyrazole, 3- {3-[2-(N,N-Dipropylamino)ethoxy]phenyl} -1 ,4-dihydroindeno[ 1,2c]pyrazole, and 3- 3 -[3-(N,N-Dipropylamino)propoxy]phenyl ,4-dihydroindeno[ 1,2c]pyrazole Dihydroxy 4-(4H-indeno-[ 1 2 -c]-pyrazol-3-yl)phenylborane 1H-[ I]Benzothieno[3,2-c]pyrazo-3-yl)benzaldehyde 1H-[ 1]Benzothieno[3 ,2-c]pyrazol-3 -yl)-N-[3-(imidazol- 1-yl)propyl]benzylamine trihydrochioride Methyl 4-(4-oxo- 1,4-dihydroindeno[1I,2-c]pyrazol-3 -yl)benzoate 4-(1 ,4-dihydroindeno[1 2 -c]pyrazol-3-yl)benzaxnide oxime 3- 4 -[(2-diethylaminoethyl)aminomethyl]phenyl} -1 ,4-dihydroindeno[ 1,2-c]pyrazole trihydrochioride WO 00/27822 WO 0027822PCTIUS99/26105 36 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenyl]benzenesulphonamide N-(2-Morpholinoethyl)-4'-dihydroindeno [1 ,2-c]pyrazol-3-ylaniline dihydrochioride 1,4-Dihydroindeno[ [1,2-c]pyrazol-6-yl)-2-morpholinoacetamide N-(2-Morpholinoethyl)-3-phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-ylamine trihydrochioride 4'-(1I -Acetyl- 1 ,4-dihydroindeno[ 1 ,2-c~pyrazoI-3-y1)acetanilide 3-[4-(2-morpholinoethoxy)phenyl]- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole 1 ,2,3,4-Tetraazol-5-yl)-4-pyridyl]-4,5 -dihydro-2H-benzo[g]indazole 3-(4-Isocyanatophenyl)- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole, 2-(Diethylamnino)ethyl N-[4-(1I,4-dihydroindeno 1 ,2-c]pyrazol-3-1)phenyl]carbamate 2-Morpholinoethyl N-[4-(1I,4-dihydroindeno [1 ,2-c]pyrazol-3 -yl)phenyl]carbarnate 3-(Dibenzylamino)propyl ,4-dihydroindeno[ 1,2-clpyrazol-3yl)phenyl]carbamate 2-[Ethyl(2-hydroxyethyl)arnino]ethyl N-[4-(1I,4-dihydroindeno i1 ,2-c]pyrazol-3yl)phenyl]carbamate 2-[[2-(Dimethylarnino)ethyl](methyl)amino~ethyI 1,4-dihydroindeno[ 1,2c]pyrazol-3-yl)phenyl]carbamate 1 -Methyl-2-propoxyethyl N-[4-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)phenyl]carbamate 1-Methyltetrahydro- 1H-2-pyrrolyl)ethyl 1,4-dihydroindeno[1I,2-c]pyrazol- 3-yl)phenyl]carbamate 2- [2-(Dimethylam-ino)ethoxy] ethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)phenyl]carbamnate 2-(Diethylamino)-l1-methylethyl ,4-dihydroindeno[ 1,2-c]pyrazol-3yl)phenyl]carbamate N-[2-(Diethylamino)ethyl]-N'-[4-( 1,4-dihydroindeno 1 ,2-c]pyrazol-3 -yl)phenyl]urea ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N-(2-morpholinoethyl)urea Ni 1,4-Dihydroindeno [1 ,2-c]pyrazol-3-yl)phenyl]-l1-piperidinecarboxamide 1,4-Dihydroindeno[ 1,2-clpyrazol-3 -yl)phenyl]-N'-[2-(dimethylamino)- 1methylethylurea 1,4-Dihydroindeno[El,2-c]pyrazol-3-yl)phenyl]-N'-tetrahydro-2furanylmethylurea WO 00/27822PC/S9210 PCT/US99/26105 37 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N'-(2-furylmethyl)urea 1,3 -Benzodioxol-5-ylmethyl)-N'-(4-( 1,4-dihydroindeno 1,2-c]pyrazol-3yl)phenyl]urea N-Cyclobutyl-AP-[4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl] 1,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)phenyl]-N'-(2-piperidinoethyl)urea urea N-Benzyl-N-[4-(1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenyl]urea N-II4-(Diethylamnino)buty]-N'P [4-(1I,4-dihydroindeno[ [1, 2 -c]pyrazol-3-yl)phenyl]urea 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)phenyl]-N'-[2-(2-thienyl)ethyl]urea N- [3-(Diethylamino)propyl]-N'"-[4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3 yl)phenyl]urea 1,4-Dihydroindeno[ [1,2-c]pyrazol-3-yl)phenyl]-N-[(1 -ethyltetrahydro- 1H-2pyrrolyl)methyl]urea -Difluorobenzyl)-N'-[4-( 1,4-dihydroindeno[ 1 2 -c]pyrazol-3-yl)phenyl]urea 1,4-Dihydroindeno[1I,2-c]pyrazol-3 -yl)phenyl]-N-[2-(2hydroxyethoxy)ethyl]urea ,4-Dihydroindeno[ [1,2-c]pyrazol-3 -yi)phenyl] -N-[2-hydroxy- 1 (hydroxymethyl)ethyl]urea 1,4-Dihydroindeno[ 1 2 -c]pyrazol-3-yl)phenyl]-N-(2,3-dihydroxypropyl)urea Ni 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yI)phenyl]-4-(2-pyridyl)- 1 pip erazinecarboxamide 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)phenyl]-N- [3-(dimethylamino)propyl]- N-methylurea Ni 1,4-Dihydroindeno [1 ,2-c]pyrazol-3-yl)phenyl]-lI-azetanecarboxamide Ni 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)phenyl]-4-(4-fluorophenyl)- 1 pip erazinecarboxamide N-Benzyl-N'-[4-( 1,4-dihydroindeno[1I,2-c]pyrazol-3 -yl)phenyl]-N-methylurea 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N-ethyl-N-(2hydroxyethyl)urea NI 1,4-Dihydroindeno[ 1 2 -c]pyrazol-3-yl)phenyl]-4-(2-methoxyphenyl)- 1piperazinecarboxamide 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenyl]-N-[2-(dimethylamino)ethyl]- N-methylurea WO 00/27822 WO 0027822PCT/US99/26105 38 NI 1,4-Dihydroindeno[ 1 ,2-clpyrazol-3 -yl)phenyl]-4-mnethyl- 1 piperazinecarboxamide NI 1,4-Dihydroindeno[ 1 ,2-cjlpyrazol-3-y1)pheny1]-4-(4-hydroxyphenl)- 1 piperazinecarboxamide Ni 1,4-Dihydroindeno[ I ,2-c]pyrazol-3 -yl)phenyl]-4-[(E)-3-phenyl-2-propenyll- I -piperazinecarboxamide NI 1,4-Dihydroindeno[ I ,2-cjpyrazo-3-y)pheny1]-4-pheflyl- I piperazinecarboxamide 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)phenyl]-NN-di(2-methoxyethyl)urea 1,4-Dihydroindeno[ I ,2-c]pyrazol-3 -yl)phenyl]-N-(2,3-dihydroxypropyl)-Nmethylurea N,N-di [2-(Diethylamino)ethyl]-M-[4-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3yl)phenyl]urea 1,4-Dihydroindeno[ 1,2-clpyrazol-3-yl)phenyl]-N-(2-pyridylmethyl)urea 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N-(3-pyridylmethyl)urea N-[4-(1I,4-Dihydroindeno[ 1,2-clpyrazol-3-yl)phenyl]-N-(4-pyridylmethyl)urea 1,4-Dihydroindeno 1,2-c]pyrazol-3-yl)phenyl] -N-(2-hydroxyethyl)urea 1,4-Dihydroindeno [1 ,2-clpyrazol-3-yl)phenyl]-NY-[7- (dimethylamino)heptyllurea and pharmaceutically acceptable salts thereof and tautomers thereof.

Additional specific compounds of formula I as represented by formula are shown in the Tables below. Pharmaceutically acceptable salts and tautomers; of these compounds are also included in the present invention.

WO 00/27822 WO 0027822PCT/US99/26105 R2= X=(CH2)n TABLE 1 R3 R4 R5R6 nRx H 1

N

2-S ,5N0 2 H 1 3-N02? 6,7-(OMe)2 I 3-OPh H 14-SMe H 1 N

CF

3 H 13-OH,4-OMe H 14-OH,3-OMe H 12-OH 6,7-(OMe)2 I4-NMC2

H

3,4 6,7,8-(OMe)3 1 2,3,4-(OMe)3 H 1 4-OCHF2 H 1 4-OH, 3,5-(But )2 H 2 4-NHAc H 2 4-OCH2 CH=CH2 H 2 4-NEt2 H 2 4-N02 H 2 4-OCH2Ph H 2 4-CN H 2 2-Cl, 5-N02 WO 00/27822 WO 0027822PCT/US99/261 X=(CH2)n TABLE 2 R3 R4 R5R6 nR2 6-AcNH1 0 H 1 I F 3 H 1Br 7-OMe 2I 0 Me H 2 i I 0 -,Me WO 00/27822 WO 0027822PCTIUS99/261 TABLE 3 R3 R4R5 R6 x R2 H C=0

O

0

___CF

3

R

1

=H

R2 X R3,R4,R5,R6 4-(Ph)PHENYL CH, H 4-(OPh)PHENYL CH 2

H

4-(SO 2 Me)PHENYL (CH 2 2 R4 MeO 3-(CO 2 H),4-(OH)PHENYL CH 2

H

2 ),4-(OH)PHENYL CH 2

H

3-(OOH 2 C0NH 2 )PHENYL CH 2

H

4-(OCH 2 Ph)PHENYL OH 2 R4,R5 (MeO) 2 4-(OCH 2 Ph)PHENYL OH 2 R5 MeO 4-(OH)PHENYL

OH

2 R4, R5 (MeO) 2 3-(CONH(OH 2 2 MOR),4-

CH

2 H (OH))PHENYL 3-(CONH(CH 2 2 NEt 2 OH 2 H

(OH)PHENYL

4-(Br)PHENYL OH 2

H

4-(OH)PHENYL OH 2 R4 MeC 4-(CONH(CH 2 3 NEt 2 HH

PHENYLOHH

4-(CONH(CH 2 2 OMe)

OH

2 H 4-(OONH(4-NO 2 Ph))

OH

2 H PHENYL__ 4-(OH)PHENYL

OH

2 R5 =MeC 4-(CONH(CH 2 2 NEt 2 OH 2 R4 =NH 2 4-(Br)PHENYL

SH

4-(OH)PHENYL

OH

2 R3 =O(0H 2 2 OMe WO 00/27822 WO 0027822PCT/US99/261 4-(SONH(CH 2 2 MOR)

CH

2 H 4-(SO 2

NH(CH

2 2 OMe)

HH

PHENYLOHH

3-(CH 2 NMe 2 OH 2 H 4-(OH)PHENYL

OH

2 R3=O(CH 2 2

MOR

4-(SO 2

NH(CH

2 2 NEt 2 OH 2 H PHENYL 4-(OOH 2 C0NH 2 OH 2 R3=0(CH)Oe PHENYL 22~ 4-(OH)PHENYL CH 2 R4=(OH), 2 2 OMe 4-(OH)PHENYL

OH

2 R4=(O(0H 2 ),OMe 4-(CONH(CH 2 2 NHEt)

OH

2 H

PHENYL

4-(CONHOH 2 -2-PYRR)PHENYL

OH

2

H

4-(OCH 2 00NH 2 )PHENYL

OH

2 J R3 =OH 4-(OCH 2 C0 2 H)PHENYL

OH

2 J H N,

R

X

NRR'

CH2 NHn-C12H25 CH2 CH2 CH2 H CH2 CH2HN-

N

CH2 WO 00/27822 WO 0027822PCT/US99/26 105 CH2 HN rH

NN

CH2 HN 0H2H CH2 HN~f oN o N NWe pN 0 HN- o~Ne

NN

o THN_, NH (HCI) HN _H o y~N o HN s

H

S HN-0j S

HN-KJN

CH2 NHiso-Pr WO 00/27822 WO 0027822PCTLUS99/26 105 CH2 NHcyc-PR CH2 NHn-Hex CH2 HN NH CH2 N CH2 N NWe CH2 N \/0 0H2 HN OMe CH2 HN Q OH

N

CH2 HN /o

N

CH2 H N 02 CH2 HN/-C N N/ -N CH2

NH

HN

o NQ o

HN

o HN, (2HCL) WO 00/27822 PCT/US99/26105

H

N, N X NRR' CH2 N /o (2HCI) CH2 N CH2 N NMe CH2 HN CH2 HN CH2 HN The present invention further includes the use of these compounds in pharmaceutical compositions with a pharmaceutically effective amount of the abovedescribed compounds and a pharmaceutically acceptable carrier or excipient. These pharmaceutical compositions can be administered to individuals to slow or halt the process of angiogenesis in angiogenesis-aided diseases, or to treat edema, effusions, exudates, or ascites and other conditions associated with vascular hyperpermeability.

DETAILED DESCRIPTION OF THE INVENTION The compounds of this invention have antiangiogenic properties. These antiangiogenic properties are due at least in part to the inhibition of protein tyrosine kinases essential for angiogenic processes. For this reason, these compounds can be used as active agents against such disease states as arthritis, atherosclerosis, psoriasis, hemangiomas, myocardial angiogenesis, coronary and cerebral collaterals, WO 00/27822 PCT/US99/26105 46 ischemic limb angiogenesis, wound healing, peptic ulcer Helicobacter related diseases, fractures, cat scratch fever, rubeosis, neovascular glaucoma and retinopathies such as those associated with diabetic retinopathy, retinopathy of prematurity, or age-related macular degeneration. In addition, some of these compounds can be used as active agents against solid tumors, malignant ascites, hematopoietic cancers and hyperproliferative disorders such as thyroid hyperplasia (especially Grave's disease), and cysts (such as hypervascularity of ovarian stroma characteristic of polycystic ovarian syndrome (Stein-Leventhal syndrome)) since such diseases require a proliferation of blood vessel cells for growth and/or metastasis.

Further, some of these compounds can be used as active agents against bums, chronic lung disease, stroke, polyps, anaphylaxis, chronic and allergic inflammation, ovarian hyperstimulation syndrome, brain tumor-associated cerebral edema, highaltitude, trauma or hypoxia induced cerebral or pulmonary edema, ocular and macular edema, ascites, and other diseases where vascular hyperpermeability, effusions, exudates, protein extravasation, or edema is a manifestation of the disease.

The compounds will also be useful in treating disorders in which protein extravasation leads to the deposition of fibrin and extracellular matrix, promoting stromal proliferation fibrosis, cirrhosis and carpal tunnel syndrome).

VEGF's are unique in that they are the only angiogenic growth factors known to contribute to vascular hyperpermeability and the formation of edema.

Indeed, vascular hyperpermeability and edema that is associated with the expression or administration of many other growth factors appears to be mediated via VEGF production. Inflammatory cytokines stimulate VEGF production. Hypoxia results in a marked upregulation of VEGF in numerous tissues, hence situations involving infarct, occlusion, ischemia, anemia, or circulatory impairment typically invoke VEGF/VPF mediated responses. Vascular hyperpermeability, associated edema, altered transendothelial exchange and macromolecular extravasation, which is often accompanied by diapedesis, can result in excessive matrix deposition, aberrant stromal proliferation, fibrosis, etc. Hence, VEGF-mediated hyperpermeability can significantly contribute to disorders with these etiologic features.

WO 00/27822 PCT/US99/26105 47 It is envisaged that the disorders listed above are mediated to a significant extent by protein tyrosine kinase activity involving the KDR/VEGFR-2 and/or the Flt-1/VEGFR-1 tyrosine kinases. By inhibiting the activity of these tyrosine kinases, the progression of the listed disorders is inhibited because the angiogenic or vascular hyperpermeability component of the disease state is severely curtailed. The action of the compounds of this invention, by their selectivity for specific tyrosine kinases, result in a minimization of side effects that would occur if less selective tyrosine kinase inhibitors were used.

The compounds of this invention have inhibitory activity against protein kinases. That is, these compounds modulate signal transduction by protein kinases.

Compounds of this invention inhibit protein kinases from serine/threonine and tyrosine kinase classes. In particular, these compounds selectively inhibit the activity of the KDR/FLK-1/VEGFR-2 tyrosine kinases. Certain compounds of this invention also inhibit the activity of additional tyrosine kinases such as Flt- 1/VEGFR-1, Src-subfamily kinases such as Lck, Src, fyn, yes, etc. Additionally, some compounds of this invention significantly inhibit serine/threonine kinases such as CDKs which play an essential role in cell-cycle progression. The potency and specificity of the generic compounds of this invention towards a particular protein kinase can often be altered and optimized by variations in the nature, number and arrangement of the substituents R1, R2, R3, R4, R5 and R6) and conformational restrictions. In addition, the metabolites of certain compounds may also possess significant protein kinase inhibitory activity.

The compounds of this invention, when administered to individuals in need of such compounds, inhibit vascular hyperpermeability and the formation of edema in these individuals. These compounds act, it is believed, by inhibiting the activity of KDR tyrosine kinase which is involved in the process of vascular hyperpermeability and edema formation. The KDR tyrosine kinase may also be referred to as FLK-1 tyrosine kinase, NYK tyrosine kinase or VEGFR-2 tyrosine kinase. KDR tyrosine kinase is activated when vascular endothelial cell growth factor (VEGF) or another activating ligand (such as VEGF-C, VEGF-D or HIV Tat protein) binds to a KDR tyrosine kinase receptor which lies on the surface of vascular endothelial cells. Following such KDR tyrosine kinase activation, WO 00/27822 PCTIUS99/26105 48 hyperpermeability of the blood vessels occurs and fluid moves from the blood stream past the blood vessel walls into the interstitial spaces, thereby forming an area of edema. Diapedesis also often accompanies this response. Similarly, excessive vascular hyperpermeability can disrupt normal molecular exchange across the endothelium in critical tissues and organs lung and kidney), thereby causing macromolecular extravasation and deposition. Following this acute response to KDR stimulation which is believed to facilitate the subsequent angiogenic process, prolonged KDR tyrosine kinase stimulation results in the proliferation and chemotaxis of vascular endothelial cells and formation of new vessels. By inhibiting KDR tyrosine kinase activity, either by blocking the production of the activating ligand, by blocking the activating ligand binding to the KDR tyrosine kinase receptor, by preventing receptor dimerization and transphosphorylation, by inhibiting the enzyme activity of the KDR tyrosine kinase (inhibiting the phosphorylation function of the enzyme) or by some other mechanism that interrupts its downstream signaling Mukhopedhyay et al., Cancer Res. 58:1278-1284 (1998) and references therein), hyperpermeability, as well as associated extravasation, subsequent edema formation and matrix deposition, and angiogenic responses, may be inhibited and minimized.

One group of preferred compounds of this invention have the property of inhibiting KDR tyrosine kinase activity without significantly inhibiting Flt-1 tyrosine kinase activity (Flt-1 tyrosine kinase is also referred to as VEGFR-1 tyrosine kinase). Both KDR tyrosine kinase and Fit-1 tyrosine kinase are activated by VEGF binding to KDR tyrosine kinase receptors and to Flt-1 tyrosine kinase receptors, respectively. Since Flt-1 tyrosine kinase activity may mediate important events in endothelial maintenance and vascular function, an inhibition of this enzyme activity may lead to toxic or adverse effects. At the very least, such inhibition is unnecessary for blocking the angiogenic responses, induction of vascular hyperpermeability and the formation of edema, so it is wasteful and of no value to the individual. Certain preferred compounds of this invention are unique because they inhibit the activity of one VEGF-receptor tyrosine kinase (KDR) that is activated by activating ligands but do not inhibit other receptor tyrosine kinases, such as Fit-i, that are also activated by certain activating ligands. The preferred WO 00/27822 PCT/US99/26105 49 compounds of this invention are, therefore, selective in their tyrosine kinase inhibitory activity.

The compounds of the present invention are also useful in the treatment of ulcers bacterial, fungal, Mooren ulcers and ulcerative colitis.

The compounds of the present invention are also useful in the treatment of conditions wherein undesired angiogenesis, edema, or stromal deposition occurs in viral infections such as Herpes simplex, Herpes Zoster, AIDS, Kaposi's sarcoma, protozoan infections and toxoplasmosis, endometriosis, ovarian hyperstimulation syndrome, systemic lupus, sarcoidosis, synovitis, Crohn's disease, sickle cell anaemia, Lyme's disease, pemphigoid, Paget's disease, hyperviscosity syndrome, Osler-Weber-Rendu disease, chronic inflammation, chronic occlusive pulmonary disease, asthma, rheumatoid arthritis and osteoarthritis, and edema following trauma, radiation, or stroke.

The compounds of the present invention are also useful in the treatment of ocular conditions such as ocular and macular edema, ocular neovascular disease, scleritis, radial keratotomy, uveitis, vitritis, myopia, optic pits, chronic retinal detachment, post-laser complications, conjunctivitis, Stargardt's disease and Eales disease in addition to retinopathy and macular degeneration.

The compounds of the present invention are also useful in the treatment of cardiovascular conditions such as atherosclerosis, restenosis, vascular occlusion and carotid obstructive disease.

The compounds of the present invention are also useful in the treatment of cancer related indications such as solid tumors, sarcomas (especially Ewing's sarcoma and osteosarcoma), retinoblastoma, rhabdomyosarcomas, neuroblastoma, hematopoietic malignancies, including leukaemia and lymphoma, tumor-induced pleural or pericardial effusions, and malignant ascites.

The compounds of the present invention are also useful in the treatment of diabetic conditions such as glaucoma, diabetic retinopathy and microangiopathy.

It is envisaged that the disorders listed above are mediated to a significant extent by protein tyrosine kinase activity involving the VEGF receptors

KDR

and Flt-1). By inhibiting the activity of these receptor tyrosine kinases, the progression of the listed disorders is inhibited because the angiogenic component of WO 00/27822 PCT/US99/26105 the disease state is severely curtailed. The action of the compounds of this invention, by their selectivity for specific tyrosine kinases, result in a minimization of side effects that would occur if less selective tyrosine kinase inhibitors were used.

In another aspect, the present invention provides compounds of formula I as defined initially above (including the provisos) for use as medicaments, particularly as inhibitors of protein kinase activity for example tyrosine kinase activity, serine kinase activity and threonine kinase activity. In yet another aspect, the present invention provides the use of compounds of formula I as defined initially above (including the provisos) in the manufacture of a medicament for use in the inhibition of protein kinase activity.

In this invention, the following definitions are applicable: "Pharmaceutically acceptable salts" refers to those salts which retain the biological effectiveness and properties of the free bases and which are obtained by reaction with inorganic acids such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid or organic acids such as sulfonic acid, carboxylic acid, organic phosphoric acid, methanesulfonic acid, ethanesulfonic acid, ptoluenesulfonic acid, salicylic acid, lactic acid, tartaric acid and the like.

"Alkyl" refers to a saturated aliphatic hydrocarbon, including straight-chain and branched-chain groups having 1 to 4 carbons.

"Alkoxy" refers to an "O-alkyl" group, where "alkyl" is defined as described above.

Pharmaceutical Formulations The compounds of this invention can be administered to a human patient by themselves or in pharmaceutical compositions where they are mixed with suitable carriers or excipient(s) at doses to treat or ameliorate vascular hyperpermeability, edema and associated disorders. Mixtures of these compounds can also be administered to the patient as a simple mixture or in suitable formulated pharmaceutical compositions. A therapeutically effective dose further refers to that amount of the compound or compounds sufficient to result in the prevention or attenuation of inappropriate neovascularization, progression of hyperproliferative disorders, edema, VEGF-associated hyperpermeability and/or VEGF-related WO 00/27822 PCTIUS99/26105 51 hypotension. Techniques for formulation and administration of the compounds of the instant application may be found in "Remington's Pharmaceutical Sciences," Mack Publishing Co., Easton, PA, latest edition.

Routes of Administration Suitable routes of administration may, for example, include oral, eyedrop, rectal, transmucosal, topical, or intestinal administration; parenteral delivery, including intramuscular, subcutaneous, intramedullary injections, as well as intrathecal, direct intraventricular, intravenous, intraperitoneal, intranasal, or intraocular injections.

Alternatively, one may administer the compound in a local rather than a systemic manner, for example, via injection of the compound directly into an edematous site, often in a depot or sustained release formulation.

Furthermore, one may administer the drug in a targeted drug delivery system, for example, in a liposome coated with endothelial cell-specific antibody.

Composition/Formulation The pharmaceutical compositions of the present invention may be manufactured in a manner that is itself known, by means of conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping or lyophilizing processes.

Pharmaceutical compositions for use in accordance with the present invention thus may be formulated in conventional manner using one or more physiologically acceptable carriers comprising excipients and auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutically. Proper formulation is dependent upon the route of administration chosen.

For injection, the agents of the invention may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks's solution, Ringer's solution, or physiological saline buffer. For transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the formulation.

Such penetrants are generally known in the art.

WO 00/27822 PCT/US99/26105 52 For oral administration, the compounds can be formulated readily by combining the active compounds with pharmaceutically acceptable carriers well known in the art. Such carriers enable the compounds of the invention to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions and the like, for oral ingestion by a patient to be treated.

Pharmaceutical preparations for oral use can be obtained by combining the active compound with a solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium carboxymethylcellulose, and/or polyvinylpyrrolidone (PVP). If desired, disintegrating agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate.

Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures.

Dyestuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.

Pharmaceutical preparations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers may be added. All formulations for oral administration should be in dosages suitable for such administration.

For buccal administration, the compositions may take the form of tablets or lozenges formulated in conventional manner.

WO 00/27822 PCT/US99/26105 53 For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebulizer, with the use of a suitable propellant, dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of e.g. gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch.

The compounds may be formulated for parenteral administration by injection, e.g. bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, in ampoules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents.

Pharmaceutical formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form. Additionally, suspensions of the active compounds may be prepared as appropriate oily injection suspensions.

Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes.

Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran.

Optionally, the suspension may also contain suitable stabilizers or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions.

Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, sterile pyrogen-free water, before use.

The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, containing conventional suppository bases such as cocoa butter or other glycerides.

In addition to the formulations described previously, the compounds may also be formulated as a depot preparation. Such long acting formulations may be WO 00/27822 PCT/US99/26105 54 administered by implantation (for example subcutaneously or intramuscularly or by intramuscular injection). Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.

An example of a pharmaceutical carrier for the hydrophobic compounds of the invention is a cosolvent system comprising benzyl alcohol, a nonpolar surfactant, a water-miscible organic polymer, and an aqueous phase. The cosolvent system may be the VPD co-solvent system. VPD is a solution of 3% w/v benzyl alcohol, 8% w/v of the nonpolar surfactant polysorbate 80, and 65% w/v polyethylene glycol 300, made up to volume in absolute ethanol. The VPD co-solvent system consists of VPD diluted 1:1 with a 5% dextrose in water solution. This co-solvent system dissolves hydrophobic compounds well, and itself produces low toxicity upon systemic administration. Naturally, the proportions of a co-solvent system may be varied considerably without destroying its solubility and toxicity characteristics. Furthermore, the identity of the co-solvent components may be varied: for example, other low-toxicity nonpolar surfactants may be used instead of polysorbate 80; the fraction size of polyethylene glycol may be varied; other biocompatible polymers may replace polyethylene glycol, e.g. polyvinyl pyrrolidone; and other sugars or polysaccharides may substitute for dextrose.

Alternatively, other delivery systems for hydrophobic pharmaceutical compounds may be employed. Liposomes and emulsions are well known examples of delivery vehicles or carriers for hydrophobic drugs. Certain organic solvents such as dimethysulfoxide also may be employed, although usually at the cost of greater toxicity. Additionally, the compounds may be delivered using a sustained-release system, such as semipermeable matrices of solid hydrophobic polymers containing the therapeutic agent. Various sustained-release materials have been established and are well known by those skilled in the art. Sustained-release capsules may, depending on their chemical nature, release the compounds for a few weeks up to over 100 days. Depending on the chemical nature and the biological stability of the therapeutic reagent, additional strategies for protein stabilization may be employed.

WO 00/27822 PCT/US99/26105 The pharmaceutical compositions also may comprise suitable solid or gel phase carriers or excipients. Examples of such carriers or excipients include but are not limited to calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols.

Many of the organic molecule compounds of the invention may be provided as salts with pharmaceutically compatible counterions. Pharmaceutically compatible salts may be formed with many acids, including but not limited to hydrochloric, sulfuric, acetic, lactic, tartaric, malic, succinic, etc. Salts tend to be more soluble in aqueous or other protonic solvents than are the corresponding free base forms.

Effective Dosage Pharmaceutical compositions suitable for use in the present invention include compositions wherein the active ingredients are contained in an effective amount to achieve its intended purpose. More specifically, a therapeutically effective amount means an amount effective to prevent development of or to alleviate the existing symptoms of the subject being treated. Determination of the effective amounts is well within the capability of those skilled in the art.

For any compound used in the method of the invention, the therapeutically effective dose can be estimated initially from cellular assays. For example, a dose can be formulated in cellular and animal models to achieve a circulating concentration range that includes the IC 50 as determined in cellular assays the concentration of the test compound which achieves a half-maximal inhibition of a given protein kinase activity). In some cases it is appropriate to determine the IC,, in the presence of 3 to 5% serum albumin since such a determination approximates the binding effects of plasma protein on the compound. Such information can be used to more accurately determine useful doses in humans. Further, the most preferred compounds for systemic administration effectively inhibit protein kinase signaling in intact cells at levels that are safely achievable in plasma.

A therapeutically effective dose refers to that amount of the compound that results in amelioration of symptoms in a patient. Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, for determining the maximum tolerated dose WO 00/27822 PCT/US99/26105 56 (MTD) and the ED 5 o (effective dose for 50% maximal response). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio between MTD and ED 50 Compounds which exhibit high therapeutic indices are preferred. The data obtained from these cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED,, with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized.

The exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition. (See e.g. Fingl et al., 1975, in "The Pharmacological Basis of Therapeutics", Ch. I pl). In the treatment of crises, the administration of an acute bolus or an infusion approaching the MTD may be required to obtain a rapid response.

Dosage amount and interval may be adjusted individually to provide plasma levels of the active moiety which are sufficient to maintain the kinase modulating effects, or minimal effective concentration (MEC). The MEC will vary for each compound but can be estimated from in vitro data; e.g. the concentration necessary to achieve 50-90% inhibition of protein kinase using the assays described herein.

Dosages necessary to achieve the MEC will depend on individual characteristics and route of administration. However, HPLC assays or bioassays can be used to determine plasma concentrations.

Dosage intervals can also be determined using the MEC value. Compounds should be administered using a regimen which maintains plasma levels above the MEC for 10-90% of the time, preferably between 30-90% and most preferably between 50-90% until the desired amelioration of symptoms is achieved. In cases of local administration or selective uptake, the effective local concentration of the drug may not be related to plasma concentration.

The amount of composition administered will, of course, be dependent on the subject being treated, on the subject's weight, the severity of the affliction, the manner of administration and the judgment of the prescribing physician.

WO 00/27822 PCT/US99/26105 57 Packaging The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient.

The pack may for example comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration.

Compositions comprising a compound of the invention formulated in a compatible pharmaceutical carrier may also be prepared, placed in an appropriate container, and labeled for treatment of an indicated condition.

In some formulations it may be beneficial to use the compounds of the present invention in the form of particles of very small size, for example as obtained by fluid energy milling.

In the compositions of the present invention the active compound may, if desired, be associated with other compatible pharmacologically active ingredients.

For example, the compounds of this invention can be administered in combination with one or more additional pharmaceutical agents that inhibit or prevent the production of VEGF, attenuate intracellular responses to VEGF, block intracellular signal transduction, inhibit vascular hyperpermeability, reduce inflammation, or inhibit or prevent the formation of edema or neovascularization. The compounds of the invention can be administered prior to, subsequent to or simultaneously with the additional pharmaceutical agent, whichever course of administration is appropriate.

The additional pharmaceutical agents include but are not limited to anti-edemic steroids, NSAIDS, ras inhibitors, anti-TNF agents, anti-ILl agents, antihistamines, PAF-antagonists, COX-1 inhibitors, COX-2 inhibitors, NO synthase inhibitors, PKC inhibitors and PI3 kinase inhibitors. The compounds of the invention and the additional pharmaceutical agents act either additively or synergistically. Thus, the administration of such a combination of substances that inhibit angiogenesis, vascular hyperpermeability and/or inhibit the formation of edema can provide greater relief from the deleterious effects of a hyperproliferative disorder, angiogenesis, vascular hyperpermeability or edema than the administration of either substance alone. In the treatment of malignant disorders, combinations with antiproliferative or cytotoxic chemotherapies or radiation are anticipated.

WO 00/27822 PCT/US99/26105 58 The present invention also comprises the use of a compound of formula I as a medicament.

Both the Src and Syk families ofkinases play pivotal roles in the regulation of immune function. The Src family currently includes Fyn, Lck, Fgr, Fes, Lyn, Src, Yes, Hck, and Blk. The Syk family is currently understood to include only Zap and Syk. The Janus family of kinases is involved in the transduction of growth factor and proinflammatory cytokine signals through a number of receptors. Although BTK and ITK, members of the Tec family of kinases, play a less well understood role in immunobiology, their modulation by an inhibitor may prove therapeutically beneficial. The kinases RIP, IRAK-1, IRAK-2, NIK, IKK-1 and IKK-2 are involved in the signal transduction pathways for the key pro-inflammatory cytokines TNF and IL-1. By virtue of their ability to inhibit one or more of these kinases, compounds of formula I may function as immunomodulatory agents useful for the maintenance of allografts and the treatment of autoimmune disorders. Through their ability to regulate T cell activation or the potentiation of an inflammatory process, these compounds could be used to treat such autoimmune diseases. Transplants due to rejection phenomena, either host versus graft for solid organs or graft versus host for bone marrow, are limited by the toxicity of currently available immunosuppressive agents and would benefit from an efficacious drug with improved therapeutic index.

Gene targeting experiments have demonstrated the essential role of Src in the biology ofosteoclasts, the cells responsible for bone resorption. Compounds of formula I, through their ability to regulate Src, may also be useful in the treatment of osteoporosis, osteopetrosis, Paget's disease, tumor-induced hypercalcemia and in the treatment of bone metastases.

A number of protein kinases have been demonstrated to be protooncogenes.

Chromosome breakage (at the Itk kinase break point on chromosome translocation as in the case of the Abl gene with BCR (Philadelphia chromosome), truncation in instances such as c-Kit or EGFR, or mutation Met) result in the creation of dysregulated proteins converting them from protooncogene to oncogene products. In other tumors, oncogenesis is driven by an autocrine or paracrine ligand/growth factor receptor interactions. Members of the src-family kinases are typically involved in downstream signal transduction thereby potentiating the t WO 00/27822 PCT/US99/26105 59 oncogenesis and they may become oncogenic by over-expression or mutation. By inhibiting the protein kinase activity of these proteins the disease process may be disrupted. Vascular restenosis may involve process of FGF and/or PDGF promoted smooth muscle and endothelial cell proliferation. Inhibition of FGFr or PDGFr kinase activity may be an efficacious strategy for inhibiting this phenomenon. Thus compounds of formula I which inhibit the kinase activity of normal or aberrant c-kit, c-met, c-fms, src-family members, EGFr, erbB2, erbB4, BCR-Abl, PDGFr, FGFr, and other receptor or cytosolic tyrosine kinases may be of value in the treatment of benign and neoplastic proliferative diseases.

In many pathological conditions (for example, solid primary tumors and metastases, Kaposi's sarcoma, rheumatoid arthritis, blindness due to inappropriate ocular neovascularization, psoriasis and atherosclerosis) disease progression is contingent upon persistent angiogenesis. Polypeptide growth factors often produced by the disease tissue or associated inflammatory cells, and their corresponding endothelial cell specific receptor tyrosine kinases KDR/VEGFR-2, Flt- 1/VEGFR-1, Tie-2/Tek and Tie) are essential for the stimulation of endothelial cell growth, migration, organization, differentiation and the establishment of the requisite new functional vasculature. As a result of the "vascular permeability factor" activity of VEGF in mediating vascular hyperpermeability, VEGFstimulation of a VEGFR kinase is also believed to play an important role in the formation of tumor ascites, cerebral and pulmonary edema, pleural and pericardial effusions, delayed-type hypersensitivity reactions, tissue edema and organ dysfunction following trauma, bums, ischemia, diabetic complications, endometriosis, adult respiratory distress syndrome (ARDS), post-cardiopulmonary bypass-related hypotension and hyperpermeability, and ocular edema leading to glaucoma or blindness due to inappropriate neovascularization. In addition to VEGF, recently identified VEGF-C and VEGF-D, and HIV-Tat protein can also cause a vascular hyperpermeability response through the stimulation of a VEGFR kinase. Tie-2 is expressed also in a select population ofhematopoietic stem cells in which it may play a role in their recruitment, adhesion, regulation and differentiation (Blood 89, 4317-4326 (1997)); this Tie-2 expressing population may serve as circulating angiogenic endothelial progenitors. Certain agents according to formula I WO 00/27822 PCT/US99/26105 capable of blocking the kinase activity of endothelial cell specific kinases could therefore inhibit disease progression involving these situations.

The compounds of formula I or a salt thereof or pharmaceutical compositions containing a therapeutically effective amount thereof may be used in the treatment of benign and neoplastic proliferative diseases and disorders of the immune system.

Such diseases include autoimmune diseases, such as rheumatoid arthritis, thyroiditis, type 1 diabetes, multiple sclerosis, sarcoidosis, inflammatory bowel disease, myasthenia gravis and systemic lupus erythematosus; psoriasis, organ transplant rejection (eg. kidney rejection, graft versus host disease), benign and neoplastic proliferative diseases, human cancers such as lung, breast, stomach, bladder, colon, pancreas, ovarian, prostate and rectal cancer and hematopoietic malignancies (leukemia and lymphoma), and diseases involving inappropriate vascularization for example diabetic retinopathy, retinopathy of prematurity, choroidal neovascularization due to age-related macular degeneration, and infantile hemangiomas in human beings. In addition, such inhibitors may be useful in the treatment of disorders involving VEGF mediated edema, ascites, effusions, and exudates, including for example macular edema, cerebral edema, and adult respiratory distress syndrome (ARDS).

The compounds of the present invention may also be useful in the prophylaxis of the above diseases.

A further aspect of the present invention provides the use of a compound of formula I or a salt thereof in the manufacture of a medicament for treating vascular hyperpermeability, angiogenesis-dependent disorders, proliferative diseases and/or disorders of the immune system in mammals, particularly human beings.

The present invention also provides a method of treating vascular hyperpermeability, inappropriate neovascularization, proliferative diseases and/or disorders of the immune system which comprises the administration of a therapeutically effective amount of a compound of formula I to a mammal, particularly a human being, in need thereof.

The in vitro potency of compounds in inhibiting these protein kinases may be determined by the procedures detailed below.

WO 00/27822 PCT/US99/26105 61 The potency of compounds can be determined by the amount of inhibition of the phosphorylation of an exogenous substrate synthetic peptide Songyang et al., Nature. 373:536-539) by a test compound relative to control.

KDR Tyrosine Kinase Production Using Baculovirus System: The coding sequence for the human KDR intra-cellular domain (aa789-1354) was generated through PCR using cDNAs isolated from HUVEC cells. A poly-His6 sequence was introduced at the N-terminus of this protein as well. This fragment was cloned into transfection vector pVL1393 at the Xba 1 and Not 1 site.

Recombinant baculovirus (BV) was generated through co-transfection using the BaculoGold Transfection reagent (PharMingen). Recombinant BV was plaque purified and verified through Western analysis. For protein production, SF-9 cells were grown in SF-900-II medium at 2 x 106/ml, and were infected at 0.5 plaque forming units per cell (MOI). Cells were harvested at 48 hours post infection.

Purification of KDR SF-9 cells expressing (His),KDR(aa789-1354) were lysed by adding 50 ml of Triton X-100 lysis buffer (20 mM Tris, pH 8.0, 137 mM NaC1, 10% glycerol, 1% Triton X-100, 1mM PMSF, 10g/ml aprotinin, 1 gg/ml leupeptin) to the cell pellet from 1L of cell culture. The lysate was centrifuged at 19,000 rpm in a Sorval SS-34 rotor for 30 min at 4 0 C. The cell lysate was applied to a 5 ml NiC1, chelating sepharose column, equilibrated with 50 mM HEPES, pH7.5, 0.3 M NaC1. KDR was eluted using the same buffer containing 0.25 M imidazole. Column fractions were analyzed using SDS-PAGE and an ELISA assay (below) which measures kinase activity. The purified KDR was exchanged into 25mM HEPES, pH7.5, NaC1, 5 mM DTT buffer and stored at -80 0

C.

Human Tie-2 Kinase Production and Purification The coding sequence for the human Tie-2 intra-cellular domain (aa775-1124) was generated through PCR using cDNAs isolated from human placenta as a template. A poly-His 6 sequence was introduced at the N-terminus and this construct was cloned into transfection vector pVL 1939 at the Xba 1 and Not 1 site.

WO 00/27822 PCT/US99/26105 62 Recombinant BV was generated through co-transfection using the BaculoGold Transfection reagent (PharMingen). Recombinant BV was plaque purified and verified through Western analysis. For protein production, SF-9 insect cells were grown in SF-900-II medium at 2 x 10 6 /ml, and were infected at MOI of Purification of the His-tagged kinase used in screening was analogous to that described for KDR.

Human Fit-1 Tyrosine Kinase Production and Purification The baculoviral expression vector pVL1393 (Phar Mingen, Los Angeles, CA) was used. A nucleotide sequence encoding poly-His6 was placed 5' to the nucleotide region encoding the entire intracellular kinase domain of human Flt-1 (amino acids 786-1338). The nucleotide sequence encoding the kinase domain was generated through PCR using cDNA libraries isolated from HUVEC cells. The histidine residues enabled affinity purification of the protein in a manner analogous to that for KDR and ZAP70. SF-9 insect cells were infected at a 0.5 multiplicity and harvested 48 hours post infection.

EGFR Tyrosine Kinase Source EGFR was purchased from Sigma (Cat E-3641; 500 units/50 pl) and the EGF ligand was acquired from Oncogene Research Products/Calbiochem (Cat PF011-100).

Expression of The baculoviral expression vector used was pVL1393 (Pharmingen, Los Angeles, The nucleotide sequence encoding amino acids M(H)6 LVPRS was placed 5' to the region encoding the entirety of ZAP70 (amino acids 1-619). The nucleotide sequence encoding the ZAP70 coding region was generated through PCR using cDNA libraries isolated from Jurkat immortalized T-cells. The histidine residues enabled affinity purification of the protein (vide infra). The LVPR 9 S bridge constitutes a recognition sequence for proteolytic cleavage by thrombin, enabling removal of the affinity tag from the enzyme. SF-9 insect cells were infected at a multiplicity of infection of 0.5 and harvested 48 hours post infection.

WO 00/27822 PCT/US99/26105 63 Extraction and purification of SF-9 cells were lysed in a buffer consisting of 20 mM Tris, pH 8.0, 137 mM NaCI, 10% glycerol, 1% Triton X-100, 1 mM PMSF, 1 ig/ml leupeptin, 10 Ig/ml aprotinin and 1 mM sodium orthovanadate. The soluble lysate was applied to a chelating sepharose HiTrap column (Pharmacia) equilibrated in 50 mM HEPES, pH 0.3 M NaC1. Fusion protein was eluted with 250 mM imidazole. The enzyme was stored in buffer containing 50 mM HEPES, pH 7.5, 50 mM NaCl and 5 mM

DTT.

Lck source Lck or truncated forms of Lck may be commercially obtained e.g. from Upstate Biotechnology Inc. (Saranac Lake, N.Y) and Santa Cruz Biotechnology Inc.

(Santa Cruz, or purified from known natural or recombinant sources using conventional methods.

Enzyme Linked Immunosorbent Assay (ELISA) For PTKs Enzyme linked immunosorbent assays (ELISA) were used to detect and measure the presence of tyrosine kinase activity. The ELISA were conducted according to known protocols which are described in, for example, Voller, et al., 1980, "Enzyme-Linked Immunosorbent Assay," In: Manual of Clinical Immunology, 2d ed., edited by Rose and Friedman, pp 359-371 Am. Soc. of Microbiology, Washington, D.C.

The disclosed protocol was adapted for determining activity with respect to a specific PTK. For example, preferred protocols for conducting the ELISA experiments is provided below. Adaptation of these protocols for determining a compound's activity for other members of the receptor PTK family, as well as nonreceptor tyrosine kinases, are well within the abilities of those in the art. For purposes of determining inhibitor selectivity, a universal PTK substrate random copolymer of poly(Glu 4 Tyr), 20,000-50,000 MW) was employed together with ATP (typically 5 iM) at concentrations approximately twice the apparent Km in the assay.

WO 00/27822 PCT/US99/26105 64 The following procedure was used to assay the inhibitory effect of compounds of this invention on KDR, Fit-1, Tie-2, EGFR and ZAP70 tyrosine kinase activity: Buffers and Solutions: PGT: Poly (Glu,Tyr) 4:1 Store powder at -20 0 C. Dissolve powder in phosphate buffered saline (PBS) for solution. Store Iml aliquots at -20 0 C. When making plates dilute to 250gg/ml in Gibco PBS.

Reaction Buffer: 100mM Hepes, 20mM MgC1 2 4mM MnC1 2 5mM DTT, 0.02%BSA, 200uM NaVO 4 pH 7.10 ATP: Store aliquots of 100mM at -20°C. Dilute to 20gM in water Washing Buffer: PBS with 0.1% Tween Antibody Diluting Buffer: 0.1% bovine serum albumin (BSA) in PBS TMB Substrate: mix TMB substrate and peroxide solutions 9:1 just before use or use K-Blue Substrate from Neogen Stop Solution: 1M Phosphoric Acid Procedure 1. Plate Preparation: Dilute PGT stock (50mg/ml, frozen) in PBS to a 250ig/ml. Add 125l per well of Coming modified flat bottom high affinity ELISA plates (Coring #25805-96). Add 125gl PBS to blank wells. Cover with sealing tape and incubate overnight 37°C.

Wash lx with 250pl washing buffer and dry for about 2hrs in 37 0 C dry incubator.

Store coated plates in sealed bag at 4 0 C until used.

2. Tyrosine Kinase Reaction: -Prepare inhibitor solutions at a 4x concentration in 20% DMSO in water.

-Prepare reaction buffer -Prepare enzyme solution so that desired units are in 50.tl, e.g. for KDR make to 1 ng/pl for a total of 50ng per well in the reactions. Store on ice.

-Make 4x ATP solution to 20gM from 100mM stock in water. Store on ice WO 00/27822 PCT/US99/26105 -Add 50il of the enzyme solution per well (typically 5-50 ng enzyme/well depending on the specific activity of the kinase) -Add 25Rl 4x inhibitor 's -Add 2 5 gl 4x ATP for inhibitor assay -Incubate for 10 minutes at room temperature -Stop reaction by adding 50[l 0.05N HC1 per well -Wash plate **Final Concentrations for Reaction: 5pM ATP, 5% DMSO 3. Antibody Binding -Dilute Img/ml aliquot of PY20-HRP (Pierce) antibody (a phosphotyrosine antibody) to 50ng/ml in 0.1% BSA in PBS by a 2 step dilution (100x, then 200x) -Add 100l Ab per well. Incubate 1 hr at room temp. Incubate Ihr at 4 0

C.

-Wash 4x plate 4. Color reaction -Prepare TMB substrate and add 100pl per well -Monitor OD at 650nm until 0.6 is reached -Stop with 1M Phosphoric acid. Shake on plate reader.

-Read OD immediately at 450nm Optimal incubation times and enzyme reaction conditions vary slightly with enzyme preparations and are determined empirically for each lot.

For Lck, the Reaction Buffer utilized was 100 mM MOPSO, pH 6.5, 4 mM MnCl 2 mM MgCl 2 5 mM DTT, 0.2% BSA, 200 mM NaVO 4 under the analogous assay conditions.

Compounds of formula I may have therapeutic utility in the treatment of diseases involving both identified, including those not mentioned herein, and as yet unidentified protein tyrosine kinases which are inhibited by compounds of formula I.

All compounds exemplified herein significantly inhibit KDR kinase at concentrations of 50 micromolar or below. Some compounds of this invention also WO 00/27822 PCT/US99/26105 66 significantly inhibit other PTKs such as Ick at concentrations of 50 micromolar or below.

Cdc2 source The human recombinant enzyme and assay buffer may be obtained commercially (New England Biolabs, Beverly, MA. USA) or purified from known natural or recombinant sources using conventional methods.

Cdc2 Assay The protocol used was that provided with the purchased reagents with minor modifications. In brief, the reaction was carried out in a buffer consisting of Tris pH 7.5, 100mM NaC1, ImM EGTA, 2mM DTT, 0.01% Brij, 5% DMSO and MgC12 (commercial buffer) supplemented with fresh 300 4M ATP (31 gCi/ml) and 30 gg/ml histone type IIIss final concentrations. A reaction volume of 80gL, containing units of enzyme, was run for 20 minutes at 25 degrees C in the presence or absence of inhibitor. The reaction was terminated by the addition of 120gL of 10% acetic acid. The substrate was separated from unincorporated label by spotting the mixture on phosphocellulose paper, followed by 3 washes of minutes each with 75mM phosphoric acid. Counts were measured by a betacounter in the presence of liquid scintillant.

Certain compounds of this invention significantly inhibit cdc2 at concentrations below 50 uM.

PKC kinase source The catalytic subunit of PKC may be obtained commercially (Calbiochem).

PKC kinase assay A radioactive kinase assay was employed following a published procedure (Yasuda, Kirshimoto, Tanaka, Tominaga, Sakurai, Nishizuka, Y.

Biochemical and Biophysical Research Communication 3:166, 1220-1227 (1990)).

Briefly, all reactions were performed in a kinase buffer consisting of 50 mM Tris- WO 00/27822 PCT/US99/26105 67 HCI pH7.5, 10mM MgC 2 2mM DTT, ImM EGTA, 100 jtM ATP, 8 .tM peptide, DMSO and "P ATP (8Ci/mM). Compound and enzyme were mixed in the reaction vessel and the reaction initiated by addition of the ATP and substrate mixture. Following termination of the reaction by the addition of 10 pL stop buffer (5 mM ATP in 75mM phosphoric acid), a portion of the mixture was spotted on phosphocellulose filters. The spotted samples were washed 3 times in 75 mM phosphoric acid at room temperature for 5 to 15 minutes. Incorporation of radiolabel was quantified by liquid scintillation counting.

Erk2 enzyme source The recombinant murine enzyme and assay buffer may be obtained commercially (New England Biolabs, Beverly MA. USA) or purified from known natural or recombinant sources using conventional methods.

Erk2 enzyme assay In brief, the reaction was carried out in a buffer consisting of 50 mM Tris pH 1mM EGTA, 2mM DTT, 0.01% Brij, 5% DMSO and 10 mM MgCl 2 (commercial buffer) supplemented with fresh 100 gM ATP (31 pCi/ml) and myelin basic protein under conditions recommended by the supplier. Reaction volumes and method of assaying incorporated radioactivity were as described for the PKC assay (vide supra).

In Vitro Models for T-cell Activation Upon activation by mitogen or antigen, T-cells are induced to secrete IL-2, a growth factor that supports their subsequent proliferative phase. Therefore, one may measure either production of IL-2 from or cell proliferation of, primary T-cells or appropriate T-cell lines as a surrogate for T-cell activation. Both of these assays are well described in the literature and their parameters well documented (in Current Protocols in Immunology, Vol 2, 7.10.1-7.11.2).

In brief, T-cells may be activated by co-culture with allogenic stimulator cells, a process termed the one-way mixed lymphophocyte reaction. Responder and stimulator peripheral blood mononuclear cells are purified by Ficoll-Hypaque WO 00/27822 PCT/US99/26105 68 gradient (Pharmacia) per directions of the manufacturer. Stimulator cells are mitotically inactivated by treatment with mitomycin C (Sigma) or gamma irradiation. Responder and stimulator cells are co-cultured at a ratio of two to one in the presence or absence of the test compound. Typically 10 s responders are mixed with 5 x 104 stimulators and plated (200 fl volume) in a U bottom microtiter plate (Costar Scientific). The cells are cultured in RPMI 1640 supplemented with either heat inactivated fetal bovine serum (Hyclone Laboratories) or pooled human AB serum from male donors, 5 x 10' M 2mercaptoethanol and 0.5% DMSO, The cultures are pulsed with 0.5 pCi of H thymidine (Amersham) one day prior to harvest (typically day three). The cultures are harvested (Betaplate harvester, Wallac) and isotope uptake assessed by liquid scintillation (Betaplate, Wallac).

The same culture system may be used for assessing T-cell activation by measurement of IL-2 production. Eighteen to twenty-four hours after culture initiation, the supernatants are removed and the IL-2 concentration is measured by ELISA (R and D Systems) following the directions of the manufacturer.

In-vivo Models of T-Cell Activation The in vivo efficacy of compounds can be tested in animal models known to directly measure T-cell activation or for which T-cells have been proven the effectors. T-cells can be activated in vivo by ligation of the constant portion of the T-cell receptor with a monoclonal anti-CD3 antibody In this model, BALB/c mice are given 10 Og of anti-CD3 Ab intraperitoneally two hours prior to exsanguination. Animals to receive a test drug are pre-treated with a single dose of the compound one hour prior to anti-CD3 Ab administration. Serum levels of the proinflammatory cytokines interferon-y (IFN- y) and tumor necrosis factor-a(TNF-a), indicators of T-cell activation, are measured by ELISA. A similar model employs in vivo T-cell priming with a specific antigen such as keyhole limpet hemocyanin (KLH) followed by a secondary in vitro challenge of draining lymph node cells with the same antigen. As previously, measurement of cytokine production is used to assess the activation state of the cultured cells. Briefly, C57BL/6 mice are immunized subcutaneously with 100 pg KLH emulsified in complete Freund's adjuvant (CFA) on day zero. Animals are pre-treated with the WO 00/27822 PCT/US99/26105 69 compound one day prior to immunization and subsequently on days one, two and three post immunization. Draining lymph nodes are harvested on day 4 and their cells cultured at 6 x 106 per ml in tissue culture medium (RPMI 1640 supplemented with heat inactivated fetal bovine serum (Hyclone Laboratories) 5 x 10s M 2-mercaptoethanol and 0.5% DMSO) for both twenty-four and forty-eight hours.

Culture supernatants are then assessed for the autocrine T-cell growth factor Interleukin-2 (IL-2) and/or IFN-y levels by ELISA.

Lead compounds can also be tested in animal models of human disease.

These are exemplified by experimental auto-immune encephalomyelitis (EAE) and collagen-induced arthritis (CIA). EAE models which mimic aspects of human multiple sclerosis have been described in both rats and mice (reviewed FASEB J.

5:2560-2566, 1991; murine model: Lab. Invest. 4(3):278, 1981; rodent model:J.

Immunol 146(4):1163-8, 1991 Briefly, mice or rats are immunized with an emulsion of myelin basic protein (MBP), or neurogenic peptide derivatives thereof, and CFA. Acute disease can be induced with the addition of bacterial toxins such as bordetella pertussis. Relapsing/remitting disease is induced by adoptive transfer of T-cells from MBP/ peptide immunized animals.

CIA may be induced in DBA/1 mice by immunization with type II collagen (J.

Immunol: 142(7):2237-2243). Mice will develop signs of arthritis as early as ten days following antigen challenge and may be scored for as long as ninety days after immunization. In both the EAE and CIA models, a compound may be administered either prophylactically or at the time of disease onset. Efficacious drugs should reduce severity and/or incidence.

Certain compounds of this invention which inhibit one or more angiogenic receptor PTK, and/or a protein kinase such as Ick involved in mediating inflammatory responses can reduce the severity and incidence of arthritis in these models.

Compounds can also be tested in mouse allograft models, either skin (reviewed in Ann. Rev. Immunol., 10:333-58, 1992; Transplantation: 57(12): 1701-17D6, 1994) or heart (Am.J.Anat.: 13:273, 1963). Briefly, full thickness skin grafts are transplanted from C57BL/6 mice to BALB/c mice. The grafts are examined daily, beginning at day six, for evidence of rejection. In the mouse WO 00/27822 PCT/US99/26105 neonatal heart transplant model, neonatal hearts are ectopically transplanted from C57BL/6 mice into the ear pinnae of adult CBA/J mice. Hearts start to beat four to seven days post transplantation and rejection may be assessed visually using a dissecting microscope to look for cessation of beating.

Cellular Receptor PTK Assays The following cellular assay was used to determine the level of activity and effect of the different compounds of the present invention on KDR/VEGFR2.

Similar receptor PTK assays employing a specific ligand stimulus can be designed along the same lines for other tyrosine kinases using techniques well known in the art.

VEGF-Induced KDR Phosphorylation in Human Umbilical Vein Endothelial Cells (HUVEC) as Measured by Western Blots: 1. HUVEC cells (from pooled donors) were purchased from Clonetics (San Diego, CA) and cultured according to the manufacturer directions. Only early passages were used for this assay. Cells were cultured in 100 mm dishes (Falcon for tissue culture; Becton Dickinson; Plymouth, England) using complete EBM media (Clonetics).

2. For evaluating a compound's inhibitory activity, cells were trypsinized and seeded at 0.5-1.0 x 105 cells/well in each well of 6-well cluster plates (Costar; Cambridge, MA).

3. 3-4 days after seeding, plates were 90-100% confluent. Medium was removed from all the wells, cells were rinsed with 5-10ml of PBS and incubated 18- 24h with 5ml of EBM base media with no supplements added serum starvation).

4. Serial dilutions of inhibitors were added in Iml of EBM media 5MM, or 1gM final concentration to cells and incubated for one hour at 370 C. Human recombinant VEGF 1 6 (R D Systems) was then added to all the wells in 2 ml of EBM medium at a final concentration of 50ng/ml and incubated at 37 0

C

for 10 minutes. Control cells untreated or treated with VEGF only were used to assess background phosphorylation and phosphorylation induction by VEGF.

WO 00/27822 PCT/US99/26105 71 All wells were then rinsed with 5-10ml of cold PBS containing 1mM Sodium Orthovanadate (Sigma) and cells were lysed and scraped in 200l of RIPA buffer Tris-HC1) pH7, 150mM NaC1, 1% NP-40, 0.25% sodium deoxycholate, 1mM EDTA) containing protease inhibitors (PMSF ImM, aprotinin 1 tg/ml, pepstatin lpg/ml, leupeptin 1gg/ml, Na vanadate ImM, Na fluoride 1mM) and 1 gg/ml of Dnase (all chemicals from Sigma Chemical Company, St Louis, MO).

The lysate was spun at 14,000 rpm for 30min, to eliminate nuclei.

Equal amounts of proteins were then precipitated by addition of cold ethanol (2 volumes) for a minimum of 1 hour or a maximum of overnight. Pellets were reconstituted in Laemli sample buffer containing (BioRad; Hercules, CA) and boiled for 5min. The proteins were resolved by polyacrylamide gel electrophoresis 1.5mm Novex, San Deigo, CA) and transferred onto a nitrocellulose membrane using the Novex system. After blocking with bovine serum albumin the proteins were probed overnight with anti-KDR polyclonal antibody (C20, Santa Cruz Biotechnology; Santa Cruz, CA) or with antiphosphotyrosine monoclonal antibody (4G10, Upstate Biotechnology, Lake Placid, NY) at 4 0 C. After washing and incubating for 1 hour with HRP-conjugated F(ab) 2 of goat anti-rabbit or goat-anti-mouse IgG the bands were visualized using the emission chemiluminescience (ECL) system (Amersham Life Sciences, Arlington Height, IL).

Certain examples of the present invention significantly inhibit cellular VEGFinduced KDR tyrosine kinase phosphorylation at concentrations of less than 50 gM.

In vivo Uterine Edema Model This assay measures the capacity of compounds to inhibit the acute increase in uterine weight in mice which occurs in the first few hours following estrogen stimulation. This early onset of uterine weight increase is known to be due to edema caused by increased permeability of uterine vasculature. Cullinan-Bove and Koss (Endocrinology (1993), 133:829-837) demonstrated a close temporal relationship of estrogen-stimulated uterine edema with increased expression of VEGF mRNA in the uterus. These results have been confirmed by the use of neutralizing monoclonal antibody to VEGF which significantly reduced the acute increase in uterine weight following estrogen stimulation (WO 97/42187). Hence, this system can serve as a WO 00/27822 PCT/US99/26105 72 model for in vivo inhibition of VEGF signalling and the associated hyperpermeability and edema.

Materials: All hormones were purchased from Sigma (St. Louis, MO) or Cal Biochem (La Jolla, CA) as lyophilized powders and prepared according to supplier instructions.

Vehicle components (DMSO, Cremaphor EL) were purchased from Sigma (St.

Louis, MO).

Mice (Balb/c, 8-12 weeks old) were purchased from Taconic (Germantown, NY) and housed in a pathogen-free animal facility in accordance with institutional Animal Care and Use Committee Guidelines.

Method: Day 1: Balb/c mice were given an intraperitoneal injection of 12.5 units of pregnant mare's serum gonadotropin (PMSG).

Day 3: Mice received 15 units of human chorionic gonadotropin (hCG) i.p.

Day 4: Mice were randomized and divided into groups of 5-10. Test compounds were administered by i.v. or p.o. routes depending on solubility and vehicle at doses ranging from 1-100 mg/kg. Vehicle control group received vehicle only and two groups were left untreated.

Thirty minutes later, experimental, vehicle and one of the untreated groups were given an i.p. injection of 17p-estradiol (500 gg/kg). After 2-3 hours, the animals were sacrificed by CO 2 inhalation. Following a midline incision, each uterus was isolated and removed by cutting just below the cervix and at the junctions of the uterus and oviducts. Fat and connective tissue were removed with care not to disturb the integrity of the uterus prior to weighing. Mean weights of treated groups were compared to untreated or vehicle treated groups. Significance was determined by Student's test. Non-stimulated control group was used to monitor estradiol response.

Results demonstrate that certain compounds of the present invention inhibit the formation of edema when administered systemically by various routes.

WO 00/27822 PCT/US99/26105 73 Certain compounds of this invention which are inhibitors of angiogenic receptor tyrosine kinases can also be shown active in a Matrigel implant model of neovascularization. The Matrigel neovascularization model involves the formation of new blood vessels within a clear "marble" of extracellular matrix implanted subcutaneously which is induced by the presence ofproangiogenic factor producing tumor cells (for examples see: Passaniti, et al, Lab. Investig. (1992), 67(4), 519- 528; Anat. Rec. (1997), 249(1), 63-73; Int. J. Cancer (1995), 63(5), 694-701; Vasc.

Biol. (1995), 15(11), 1857-6). The model preferably runs over 3-4days and endpoints include macroscopic visual/image scoring of neovascularization, microscopic microvessel density determinations, and hemoglobin quantitation (Drabkin method) following removal of the implant versus controls from animals untreated with inhibitors. The model may alternatively employ bFGF or HGF as the stimulus.

Certain compounds of this invention which inhibit one or more oncogenic, protooncogenic, or proliferation-dependent protein kinases, or angiogenic receptor PTK also inhibit the growth of primary murine, rat or human xenograft tumors in mice, or inhibit metastasis in murine models.

EXEMPLIFICATIONS

I. Synthesis There are two general approaches to the synthesis of the ring systems of the compounds of formula I that have been set forth in U.S. Patent 3,843,665 and U.S.

Patent 3,843,666.

In U.S. Patent 3,843,665, cyclization of the pyrazole ring is affected by heating compounds of formula II with an aromatic sulfonylhydrazide of formula III in an inert solvent and a catalytic amount of an acid. The reaction is carried out for a period of 5 to 30 hours preferably at a temperature of 75°C to 100 0 C and gives compounds of formula I in which R1 is hydrogen, WO 00/27822 PCT/US99/26105 74 R x 1X^ R NHNHSO2 RR O II III wherein: p is 0, 1, 2; Wis a lower alkyl and R 2

R

3

R

4

R

5

R

6 and X are as previously defined. Compounds of formula HI are prepared by treating an appropriately functionalized compounds of formula IV with an aldehyde of formula V in the presence of an acid or base catalyst (Braun, R. Mosher, W. A. J. Amer. Chem.

Soc. 1958, 80, 2749).

R

3 R4 X O

R

5 R2 H

R,

IV V A second method of preparing the ring systems of the compounds of formula I in which R, is hydrogen is set forth by U.S. Patent 3,843,666 where compounds with the general formula VI are heated to 750 to 175°C with a catalytic amount of an organic carboxylic acid or an organic sulfonic acid in an inert solvent such as an aromatic hydrocarbon for a period of 6 to 24 hours,

R

3 R4 X

R

R r% VI wherein: R 2

R

3

R

4

R

6 and X are as previously defined.

WO 00/27822 PCT/US99/26105 Compounds of formula VI are prepared by treating compounds of the general formula VII with hydrazine in an inert solvent. The reaction is carried out at 150 to for a period of up to 24 hours.

R3 R

VII

R

6 0 Alternatively compounds of formula I may be prepared directly by reacting a compound of formula VII with hydrazine without isolating the compound of formula VI, for example by heating a compound of formula VII with hydrazine in an inert solvent, e.g. methanol, in the presence of an acid catalyst, e.g. acetic acid, at a temperature in the range from 60°C to the boiling point of the inert solvent employed.

Compounds of formula I may also be prepared by reacting a compound of formula XVI

R

3

R

5 R2 XVI

R

6

O

wherein: R1, R 2

R

3

R

4

R

5

R

6 and X are as previously defined with hydrazine in an inert solvent e.g. methanol, at a temperature in the range of from 15C to the boiling point of the inert solvent employed.

Compounds which have the general formula VII are prepared by treating a compound of formula VIII with an aldehyde of formula V under basic conditions.

The reaction is carried out in an inert solvent at a temperature between 5°C and for a period of 3 to 6 hours, WO 00/27822 PCT/US99/26105 76

R

3 R-Y VIII R, O wherein: Y is any conventional leaving group, such as chlorine, bromine, iodine, tosylate or mesylate and R 3

R

4

R

5

R

6 and X are as previously defined.

Compounds of formula VII may also be prepared by reacting a compound of formula II with an epoxidizing agent, for example hydrogen peroxide, in an inert solvent, for example methanol, dichloromethane, water or mixtures thereof, at a temperature in the range of 00 to 100 0 C optionally in the presence of a base, for example sodium hydroxide.

Cyclization of VI can also be effected by treatment with a mineral acid such as hydrochloric acid, sulfuric acid or phosphoric acid. The reaction is carried out in a lower alkanol at a temperature between 15 0 C to 20 0 C for a period of 12 to 48 hours. The product of the reaction, IX, can then be aromatized to I by heating to a temperature of 50 0 C to 150C with an organic carboxylic acid or an organic sulfonic acid in a straight chain ether or cyclic ether for the period of 8 to 30 hours.

R

3 ^R4 ^X OH R IX R 2 R6 N

H

Compound IX can be diacetylated by treatment with an acid anhydride of formula (RxCO)20 in which R, is a C1-4 alkyl group in an inert solvent such as an aromatic hydrocarbon at a temperature between 35 0 C to 200 0 C for a period of to 8 hours to give a compound of formula XI.

WO 00/27822 PCT/US99/26105

R.

N-N

XR: 0 Compound XI can then be aromatized to compound XII by heating to a temperature of 35 0 C to 200 0 C with a mineral acid or an organic acid in an inert solvent for the period of 4 to 8 hours. Finally, compound XII can be converted to I by heating to a temperature of 50 0 C to 150 0 C in an inert solvent such as water or a lower alcohol in the presence of an alkali metal or an alkali metal hydroxide for the period of 8 to 30 hours.

N)1 xN

I

Compounds with the general formula II can be cyclized to compounds of formula XIII by reaction with hydrazine in an inert solvent, for example methanol, at a temperature in the range of 35-150°C.

R

3 R4

X

R

XIII

R

6

NH

Compounds of formula I may be prepared by reacting a compound of formula XIII with a dehydrogenating agent, for example sulphur, oxygen, palladium, manganese dioxide or lead dioxide optionally in the presence of an inert solvent, for example a hydrocarbon, at a temperature in the range of 15 to 2500C.

WO 00/27822 PCTIUS99/26105 78 Specific examples of the above transformations can be found in U.S. Patents 3,843,665 and 3,843,666.

The bridging carbonyl can be transformed to a methylene group via a Wolf- Kishner reduction of the corresponding hydrazone (Mosher, Tawfik, Lipp, D. W. J. Org. Chem. 1971, 36, 3890).

Additional methods for functionalization of the bridging carbonyl and specific examples can be found in Japanese Patent Application JP 60 130521 A2, and B. Loev, U.S. Patent 3,004,983 (1960).

Compounds of formula I may be prepared by reacting a compound of formula XIV

R

3 R XIV

R

6

N

NH

2 with a strong base, for example n-butyllithium at a temperature in the range of-78°C to 25 0 C, followed by reaction with a compound of formula R2COG (XV) in which R2 is as previously defined and G represents a C1-6 alkoxy group.

Compounds of formula IV, VIII, XIV, XV and XVI are commercially available or may be prepared by methods known to those skilled in the art.

Compounds of formula I in which X represents SO, or S02 may be prepared by oxidizing a compound of formula I in which X represents S by methods known to those skilled in the art for example by using an appropriate number of molar equivalents of 3-chloroperbenzoic acid.

Compounds of formula I in which X represents a group of formula

C=NOR

7 may be prepared by reacting a compound of formula I in which X represents carbonyl, with a compound of formula H2NOR7 by methods known to those skilled in the art.

Compounds of formula I in which R2 4-pyridyl may be further functionalized in the 2-position of the pyridine ring by methods known to those skilled in the art, for example, via pyridine-N-oxide mediated rearrangements.

WO 00/27822 PCT/US99/26105 79 Certain substituents in compounds of formula I may be interconverted by methods known to those skilled in the art. For example alkoxy substituents may be reacted with a suitable ether cleaving reagent for example hydrobromic acid, boron tribromide or pyridine hydrochloride to give a compound of formula I with a hydroxy substituent. Alternatively compounds of formula I with an alkoxy substituent may be prepared by alkylating compounds of formula I which have a hydroxy substituent. Carboxylic ester substituents may be converted into carboxy or amide substituents and carboxylic acid substituents may be converted into carboxylic ester or amide substituents. Nitro substituents may be reduced to amines and amines may be acylated by methods known to those skilled in the art.

It will be appreciated by those skilled in the art that certain substituents may react with some of the reagents described in the above processes. In such cases an alternative process should be used or the reactive substituent should be protected prior to the reaction and deprotected after the reaction.

The invention is illustrated by the following Examples which are given by way of example only. The final product of each of these Examples was characterized by one or more of the following procedures: high performance liquid chromatography; elemental analysis, nuclear magnetic resonance spectroscopy, infrared spectroscopy and high resolution mass spectroscopy. The following abbreviations are used IMS industrial methylated spirit LCMS liquid chromatography/mass spectroscopy.

Example 1 a) A mixture of indan-1-one (10.0 ethanol (35 ml), hydrazine hydrate (10.0 ml) and glacial acetic acid (2.0 ml) was boiled under reflux under nitrogen for 1 hour. The mixture was cooled to 20 0 C and the mixture concentrated under reduced pressure to give a solid which was collected by filtration to give indan-1-one hydrazone, m.p. 84-86 0

C.

b) A solution of n-butyllithium (15.0 ml of a 2.5M solution in hexane) was added dropwise to a mixture of the hydrazone from a) (1.82 g) in WO 00/27822 PCT/US99/26105 tetrahydrofuran (40 ml) at 0 C under nitrogen with stirring. The mixture was stirred at 0°C for 0.5 hours and then methyl 3 4 (1.41 g) was added dropwise over 10 minutes and the mixture was stirred at 0°C for 20 minutes. Dilute hydrochloric acid (40 ml, 3M) was added and the mixture was boiled under reflux for 1 hour. The mixture was cooled to ambient temperature and separated. The aqueous layer was neutralized with sodium bicarbonate and extracted with ether to give a brown oil. The oil was purified by flash column chromatography on silica using ethyl acetate/petroleum ether as the mobile phase to give 3-(3,4,5trimethoxyphenyl)- 1,4-dihydroindeno[ ,2-c]-pyrazole, m.p. 185-187°C.

Example 2 a) In a similar manner to Example 1, indan-1-one hydrazone (3.55g) was dissolved in tetrahydrofuran (80ml) at 0 C under nitrogen with stirring. n- Butyllithium (28.8ml of a 2.5M solution in hexane) was added to the solution and the mixture was stirred at 0°C for 0.5h. Ethyl 3-methoxybenzoate (2.16g) was added, followed by 3M hydrochloric acid (80ml) and the mixture was worked up as described in Example 1 to give 3-(3methoxyphenyl)-1,4-dihydroindeno[1,2-c]pyrazole, m.p. 172-174C.

b) A solution of boron tribromide in dichloromethane (4.58ml of a 1M solution) was added with stirring to a suspension of the product from a) (0.30g) in dichloromethane (30ml) at -40 0 C with stirring under nitrogen. The mixture was stirred at between -40 0 C and -20 0 C for 0.5h and then warmed to ambient temperature. The mixture was stirred at ambient temperature for 0.5h and then poured into methanol 100ml. Silica was added and the mixture was preabsorbed onto silica and then purified by flash column chromatography using ethyl acetate/petroleum ether (1:2.5 increasing the proportion of ethyl acetate until 100% ethyl acetate was reached and finally using ethyl acetate/methanol Appropriate fractions were collected, combined and WO 00/27822 PCT/US99/26105 81 recrystallized from ethanol to give 3-(1,4-dihydroindeno-[1,2-c]pyrazol-3yl)phenol, m.p. 302-304°C.

Example 3 A mixture of 2-benzoylbenzo[b]thiophen-3(2H)-one (1.50 g, supplied by the Maybridge Chemical Company, Tintagel, England) hydrazine hydrate (0.3 ml) and butan-1-ol (50 ml) was boiled under reflux under nitrogen for 7.5 hours. The solvent was removed under reduced pressure and the residue was taken up in ethyl acetate, washed with water, dried, filtered and evaporated to give a solid which was purified by flash column chromatography on silica using toluene/ethyl acetate as the mobile phase. Appropriate fractions were collected, combined and evaporated to give a solid which was triturated with ether, and filtered to give 3-phenyl-lH- [1]benzothieno[3,2-c]pyrazole, m.p. 236-238°C.

Example 4 A solution of 3-chloroperoxybenzoic acid (450 mg, 60% pure) in dichloromethane (30 ml) was added dropwise with stirring over 15 minutes to a solution of 3-phenyl-lH-[l]benzothieno[3,2-c]pyrazole (400 mg) in dichloromethane (50 ml) at 0-5 0 C. The mixture was stirred at 5-15 0 C for 4 hours, then washed with water, dried and evaporated to give a solid which was purified by chromatography on silica using petroleum ether/ethyl acetate as the mobile phase. Appropriate fractions were collected, combined and evaporated to give a solid which was triturated with ether, and filtered to give 3-phenyl-1H- [1]benzothieno[3,2-c]pyrazole 4-oxide, m.p. 222-224 0

C.

Example A solution of 3-chloroperoxybenzoic acid (440 mg, 60% pure) in dichloromethane (30 ml) was added dropwise with stirring over 15 minutes to a solution of 3-phenyl-1H-[ ]benzothieno[3,2-c]pyrazole (190 mg) in dichloromethane (30 ml) at 0-5 0 C. The mixture was stirred at 5-15 0 C for 4 hours with more 3-chloroperoxybenzoic acid (220 mg) being added after 2 hours. The reaction mixture was washed with water, dried, filtered and evaporated to give a WO 00/27822 PCTIUS99/26105 82 solid which was dissolved in ethyl acetate (100 ml), washed with 1M sodium hydroxide solution (50 ml), then with water and then dried, filtered and evaporated to give a solid which was triturated with ether and filtered to give 3-phenyl-1H- [1]benzothieno-[3,2-c]pyrazole 4,4-dioxide, m.p. 266-268 0

C.

Example 6 A mixture of 3-phenylindeno[1,2-c]pyrazol-4(1H)-one (1.28 g, prepared as described in JP60-130521), hydroxylamine hydrochloride (0.5 sodium acetate (0.8 water (10 ml) and methanol (100 ml) was boiled under reflux for 66 hours.

More hydroxylamine hydrochloride (0.5 sodium acetate (0.8 g) and water (10 ml) were added and the mixture was boiled under reflux for a further 16 hours. Further hydroxylamine hydrochloride (0.5 g) and sodium acetate (0.8 g) were added and the mixture was boiled under reflux for a further 20 hours. The mixture was filtered and the filtrate was concentrated under reduced pressure until crystallization began. The mixture was cooled and filtered to give a solid which was washed with water and dried to give 3-phenylindeno[1,2-c]pyrazol-4(1H)-one oxime, m.p. 280 0 C (with decomposition).

Example 7 a) A mixture ofindan-l-one (3.3 methyl 4-formylbenzoate (5.0 g), piperidine (0.6 ml) and glacial acetic acid (0.5 ml) was heated on a steam bath for 3 hours. The solid mass obtained was boiled up in industrial methylated spirits (200 ml) and then hot filtered. The solid residue obtained was washed with industrial methylated spirits and dried to give methyl 4-(1oxoindan-2-ylidenemethyl)benzoate, m.p. 194-198 0

C.

b) The product from a) (1.5 g) was suspended in methanol (10 ml) and dichloromethane (15 ml) and stirred at 0-5 0 C whilst 2M sodium-hydroxide solution (2.7 ml) was added followed by 30% hydrogen peroxide (100 vol.

1.1 ml). The mixture was stirred at 0-5 0 C for 5 minutes then at ambient temperature for 24 hours. Dichloromethane (100 ml) was added to the mixture which was then washed with brine (2 x 50 ml), dried, filtered and WO 00/27822 PCT/US99/26105 83 evaporated to give methyl 4-(1-oxospiro[indan-2,2'-oxiran]-3'-yl)benzoate, m.p. 160-163 0 C. The aqueous phase was acidified with 5M hydrochloric acid and extracted with dichloromethane to give 4-(1-oxospiro[indan-2,2'oxiran]-3'-yl)benzoic acid, m.p. 220 0 C with decomposition c) A mixture of 4-(1-oxospiro[indan-2,2'-oxiran]-3'-yl)benzoic acid (750 mg), methanol (30 ml) and hydrazine hydrate (0.16 ml) was stirred at ambient temperature whilst glacial acetic acid (6 drops) were added. The mixture was boiled under reflux for 24 hours and then allowed to stand at ambient temperature for 24 hours, then cooled to 0°C and filtered to give methyl 4- (1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzoate, m.p. 224-226 0

C.

Example 8 4-(l-Oxospiro[indan-2,2'-oxiran]-3'-yl)benzoic acid from Example 7b (780 mg), methanol (50 ml), hydrazine hydrate (0.18 ml) and glacial acetic acid (6 drops) were boiled under reflux for 24 hours. The mixture was cooled in ice and filtered to give 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzoic acid, m.p. >320 0

C.

Example 9 A mixture of 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzoic acid (1.05 g) and dry tetrahydrofuran (30 ml) was stirred at ambient temperature whilst triethylamine (1.1 ml) was added. The mixture was stirred at ambient temperature for 0.5 h then cooled to 3°C and methyl chloroformate (1.2 ml) was added dropwise over 5 to 10 minutes at 3-9 0 C. The mixture was stirred for 1 hour at 3-9 0 C and then added to concentrated aqueous ammonia solution (70 ml, SG 0.880) with rapid stirring. The mixture was stirred for 2 hours at 5 0 C and allowed to warm up to ambient temperature. The mixture was concentrated under reduced pressure and then treated with 1M sodium hydroxide solution (200 ml). The mixture was stirred for 0.5 hour and then filtered. The solid obtained was stirred with 2M sodium hydroxide solution (50 ml) for 6 hours and then allowed to stand at ambient temperature for 18 hours. The mixture was filtered to give a solid which was WO 00/27822 PCT/US99/26105 84 washed with water and dried. This solid was purified by flash column chromatography on silica using dichloromethane/industrial methylated spirit/triethylamine (25:2.5:1.5) as the mobile phase. The appropriate fractions were combined and concentrated to give a residue which was washed with water and dried under vacuum at 40 0 C to give 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzamide, m.p. 290-293 0

C.

Examples 10 and 11 a) A mixture of N-(l-oxoindan-5-yl)acetamide (10.0 g, obtained from the Maybridge Chemical Co. Ltd), benzaldehyde (6.73 glacial acetic acid (1.06 g) and piperidine (10.8 g) was heated to 90 0 C under nitrogen.

Methanol (200 ml) was added and the mixture was cooled and then filtered to give N-(2-benzylidene-l-oxo-indan-5-yl)acetamide (13.47 g).

b) The product from a) above (9.20 dichloromethane (30 ml) and methanol ml) was stirred at 20 0 C before adding 2M sodium hydroxide solution ml) and hydrogen peroxide (6.6 ml, 100 volumes) with stirring at 20 0

C.

The mixture was stirred at 20 0 C for 24 hours. Further hydrogen peroxide (3 ml, 100 volumes) was added and the mixture was stirred for 24 hours.

The mixture was neutralized with glacial acetic acid and the solid obtained was collected by filtration to give N-(l-oxo-3'-phenylspiro[indan-2,2'm.p. 195-197°C.

c) The product from b) (2.0 g) was dissolved in ethanol (30 ml) and then hydrazine hydrate (0.34 g) was added followed by glacial acetic acid drops). The mixture was boiled under reflux for 5 hours, then cooled. A solid was collected by filtration and purified by flash column chromatography on silica using dichloromethane/methanol (95:5) as the mobile phase to give N-(3-phenyl-l,4-dihydroindeno[1,2-c]pyrazol-6yl)acetamide, m.p. 275-276 0 C as Example WO 00/27822 PCT/US99/26105 Certain fractions from the chromatography contained another compound in addition to Example 10. These fractions were combined and evaporated under reduced pressure. The residue obtained was dissolved in ethyl acetate and extracted with 2M hydrochloric acid. The combined acid extracts were basified with 2M sodium hydroxide solution and filtered to give 3-phenyl- 1,4-dihydroindeno[1,2-c]pyrazol-6-ylamine, m.p. 228-230 0 C as Example 11.

Example 12 a) A mixture ofindan-1-one (20.0 4-nitrobenzaldehyde (27.0 glacial acetic acid (3.0 g) and piperidine (3.06 g) was heated at 95 0 C under nitrogen for 3.5 h. The mixture was cooled to 20 0 C and filtered to give a solid which was recrystallized from industrial methylated spirit to give 2-(4nitrobenzylidene)indan- 1-one.

b) The product from a) (28.0 g) was stirred with dichloromethane (100 ml) and methanol (100 ml) at 20 0 C and then 2M sodium hydroxide solution (50 ml) was added followed by hydrogen peroxide (20 ml, 100 volumes). The mixture was stirred at 20°C for 24 hours. Further hydrogen peroxide (10.0 ml, 100 volumes) was added and the mixture was stirred for a further 24 hours. Further hydrogen peroxide (10 ml, 100 volumes) was added and the mixture was stirred for 64 hours. The reaction mixture was neutralized with glacial acetic acid and the solid which formed was collected by filtration and dried to give 3'-(4-nitrophenyl)-l-oxospiro[indan-2,2'-oxirane].

c) The product from b) (10.0 g) was dissolved in ethanol (180 ml) and hyrazine hydrate (1.78 g) was added to the solution obtained, followed by glacial acetic acid (30 drops). The mixture was boiled under reflux for 5 hours and then cooled to 20 0 C and stood at this temperature for 18 hours. The solid was collected by filtration and recrystallized from acetone to give 3-(4nitrophenyl)-l,4-dihydroindeno[1,2-c]pyrazole, m.p. 267-270°C.

WO 00/27822 PCT/US99/26105 86 Example 13 The product from Example 12 (3.0 g) was suspended in industrial methylated spirit (200 ml) and 5% palladium on charcoal (250 mg) was added followed by ammonium formate (2.05 The mixture was stirred and heated at 70 0 C for 3 hours and then cooled to ambient temperature and then filtered. The filtrate was concentrated under reduced pressure and triturated with dichloromethane to give 4- (1,4-dihydroindeno[1,2-c]pyrazol-3-yl)aniline, m.p. 253-254 0

C.

Example 14 3-(4-Pyridyl)-4,5-dihydro-1H-benzo[g]indazole (100 mg, compound commercially available from Aldrich) was dissolved in tetrahydrofuran (5-10 ml) with warming and then 3-chloroperoxybenzoic acid (1.25 molar equivalents of pure material) was added. The mixture was heated at 55-60 0 C for 4 hours. A precipitate was collected by filtration, washed with tetrahydrofuran and then ether to give 4-(4,5-dihydro-1H-benzo[g]indazol-3-yl)pyridine 1-oxide.

Example The N-oxide product from Example 14 (150 mg) was dissolved in phosphorus oxychloride (6 ml). The mixture was boiled under reflux for 4 hours.

Excess phosphorus oxychloride was removed under reduced pressure and the residue was washed with saturated sodium carbonate solution and the product was extracted into chloroform, washed, dried and evaporated to give 3-(2-chloro-4-pyridyl)-4,5dihydro- 1H-benzo[g]indazole.

Example 16 The N-oxide product from Example 14 (25 mg) was suspended in dimethylformamide (200 gl) and to this suspension was added trimethylsilyl cyanide molar equivalents) and triethylamine (3 molar equivalents). The mixture was heated at 110°C for 12 hours and was then diluted with dichloromethane and washed with saturated sodium bicarbonate solution. The organic layer was separated, dried, filtered and evaporated to give, after flash column chromatography using 20-40% of WO 00/27822 PCT/US99/26105 87 acetonitrile in dichloromethane as the mobile phase, 4-(4,5-dihydro-1Hbenzo[g]indazol-3-yl)-2-pyridinecarbonitrile.

Example 17 The product from Example 16 (50 mg) was suspended in a mixture of ethanol (1 ml) and water (0.5 ml) containing sodium carbonate (1 molar equivalent) and hydroxylamine hydrochloride (2 molar equivalents). The mixture was boiled under reflux for 4 hours, then cooled. A precipitate formed which was collected by filtration to give 4-(4,5-dihydro-1H-benzo[g]indazol-3-yl)-2-pyridinecarboxamide oxime.

Example 18 Potassium carbonate (1 molar equivalent) was added to a mixture of the product from Example 16 (50 mg), DMSO (600 ul), followed by the addition of hydrogen peroxide (250 pl of a 30% solution) which was added dropwise. Within a few minutes of the completion of the addition a precipitate had formed. The mixture was stirred for 1 hour and then water was added. The precipitate was collected by filtration to give 4-(4,5-dihydro-lH-benzo[g]indazol-3-yl)-2-pyridine carboxamide.

Example 19 Palladium on charcoal (a spatula tip of 10% material) was added to a suspension of the product from Example 16 (50 mg) in a mixture of trifluoroacetic acid in methanol (5 ml of a 5% v/v solution of trifluoroacetic acid in methanol) and the mixture was kept in an atmosphere of hydrogen for 24 hours with stirring. The mixture was filtered to remove the catalyst and the filtrate was concentrated under reduced pressure (to around 0.5 ml). Ethereal hydrogen chloride (20 ml) was added and the precipitate which formed was collected by filtration and dried to give (4,5-dihydro-1H-benzo[g]indazol-3-yl)-2-pyridyl]methyl}ammonium chloride.

Example The product from Example 19 (100 mg) was suspended in propyl formate ml) and ethanol (1.5 ml). This mixture was boiled under reflux and after 2 WO 00/27822 PCT/US99/26105 88 minutes triethylamine (0.5 ml) was added. The mixture was boiled under reflux for 4 hours and then cooled and diluted with dichloromethane (50 ml). The mixture was washed with water, then dried, filtered and evaporated to give a residue which was triturated with ether and dichloromethane to give N-{[4-(4,5-dihydro-1H-benzo[g]indazol-3-yl)-2-pyridyl]methyl} formamide.

Examples 21-24 The following examples are commercially available or may be prepared by methods analogous to those described in examples 1-6.

Example 21 3-(3,4-Dimethoxyphenyl)indeno[ 1,2-c]pyrazol-4(1 H)-one oxime.

Example 22 3-(4-Methylphenyl)indeno[1,2-c]pyrazol-4(1H)-one oxime (available from Menai).

Example 23 3-(2-Thienyl)indeno[1,2-c]pyrazol-4(1H)-one (was commercially available or may be prepared by methods analogous to those described in JP60-130521).

Example 24 3-Phenyl-lH-benzofuro[3,2-c]pyrazole.

Example A mixture of 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzoic acid (0.8 g), methylamine in ethanol (0.27 ml of a 33% w/w solution), 4-pyrrolidinopyridine (0.47 diisopropylcarbodiimide (0.5 ml) and dichloromethane (30 ml) was stirred at ambient temperature for 20 hours. More methylamine in ethanol (0.3 ml of a 33% w/w solution), 4-pyrrolidinopyridine (0.5 g) and diisopropylcarbodiimide (1 ml) were added and the mixture was stirred for 3 hours at ambient temperature. The solvent was removed under reduced pressure at 50°C. The mixture was kept under WO 00/27822 PCT/US99/26105 89 vacuum for 2 hours and then dichloromethane (50 ml) was added. The mixture was stirred at ambient temperature for 20 hours and then allowed to stand for 72 hours.

A solid was removed by filtration and purified by flash column chromatography on silica using toluene/acetic acid (10:1) and then ethyl acetate as the mobile phase to give N-methyl-4-(1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, m.p.

182-188 0

C.

Example 26 a) Lithium diisopropylamide (27.2 ml of a 2.0M solution in heptane/THF/ ethylbenzene) was added dropwise to a stirred solution of one tert-butyloxycarbonylhydrazone (5.0 g) in tetrahydrofuran (150 ml) at -78 0 C under nitrogen with stirring. After the addition was complete the mixture was stirred for 1.5 hours at -78 0 C and then a solution of ethyl thiophen-2-carboxylate (3.39 g) in tetrahydrofuran (25 ml) was added dropwise. The mixture was stirred at -78 0 C for 1.5 hours and then allowed to warm to ambient temperature. After 30 minutes the reaction mixture was quenched by the addition of saturated ammonium chloride solution and the layers were separated. The aqueous phase was extracted with ether. The combined organic phases were washed with 2M hydrochloric acid, dried, filtered and evaporated to give a residue which was dissolved in dichloromethane (100 ml) and trifluoroacetic acid (0.35 ml) was added. The mixture was stirred under a nitrogen atmosphere at ambient temperature and then purified by flash column chromatography using ethyl acetate/petroleum ether b.p 60-80 0 C as the mobile phase to give 6-methoxy-2-tertbutyloxycarbonyl-3-(2-thienyl)-1,4-dihydroindeno[1,2-c] pyrazole.

b) A solution of boron tribromide in dichloromethane (1.35 ml of a 1M solution) was added with stirring to a solution of the product from a) (100 mg) in dichloromethane (5 ml) at -78 0 C under a nitrogen atmosphere.

The mixture was stirred for 2 hours at -78 0 C and then the mixture was allowed to warm up to ambient temperature slowly and stirred at this temperature for 18 hours. The mixture was quenched with water and the WO 00/27822 PCT/US99/26105 organic layer was separated. The aqueous layer was extracted with dichloromethane and the combined organic extracts were washed with brine, dried, filtered and evaporated to give 3-(2-thienyl)-1,4-dihydroindeno[1,2c]pyrazol-6-ol, m.p. >300 0

C.

Example 27 a) Triethylamine (0.48 ml) was added to a solution of 4-(1,4dihydroindeno[1,2-c]pyrazol-3-yl)aniline (400 mg) in dichloromethane ml) with stirring under nitrogen at ambient temperature followed by benzoyl chloride (0.4 ml). The mixture was stirred for 3 hours and then allowed to stand for 18 hours. The mixture was filtered to give benzoyl-l,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzanilide, m.p. 241 0

C.

b) The product from a) (0.35 g) was dissolved in methanol (20 ml) and 2M sodium hydroxide solution (3.85 ml) was added. The mixture was stirred at 0 C for 2 hours and then the solid was collected by filtration and dried in vacuum at 60 0 C for 2 hours to give 4'-(1,4-dihydroindeno[1,2-c]pyrazol-3yl)- benzanilide hydrate, m.p. 273-274 0

C.

Example 28 a) Methyl thiosalicylate (2.0 g) was added dropwise to a stirred solution of sodium methoxide (1.28 g) in methanol (50 ml) at ambient temperature. The mixture was stirred at ambient temperature for 15 minutes and then a solution of 2-(bromoacetyl)thiophene (2.26 g) in methanol (50 ml) was added dropwise. After the addition was complete the mixture was boiled under reflux for 3 hours, then cooled, and 10% aqueous hydrochloric acid (100 ml) was added. A solid was collected by filtration, dried and then recrystallized from ethanol to give 2-thenoylbenzo[b]thiophene-3-ol.

b) A mixture of the product from a) (500 mg), hydrazine hydrate (106 mg) and n-butanol (5 ml) was boiled under reflux under nitrogen for 7 hours. The mixture was concentrated under vacuum to give a gum which was WO 00/27822 PCTIUS99/26105 91 crystallized from ethyl acetate/petroleum ether, b.p. 60-80 0 C, to give a solid which was purified by flash column chromatography on silica using ethyl acetate/ petroleum ether, b.p. 60-80 0 C as the mobile phase.

Appropriate fractions were collected and evaporated to give 3-(2-thienyl)- 1H-benzothieno[3,2-c]pyrazole, m.p. 236-238°C.

Example 29 A mixture of methyl 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzoate (0.3 g) and N,N-diethylethylenediamine (1 ml) was heated at 150°C for 3.5 hours and then at 180 0 C for 2 hours. The mixture was cooled and dissolved in industrial methylated spirit and this solution was preabsorbed onto silica which was applied to the top of a flash column. The column was eluted with dichloromethane/industrial methylated spirit/triethylamine (25:2:1) to give a solid which was stirred with water for 1 hour, filtered and dried under vacuum at 60 0 C to give N-(2-diethylaminoethyl)- 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzamide, m.p. 172-174°C.

Example A mixture of 4-(1-oxoindan-2-ylidenemethyl)phenol (1.67 g, Chem. Ber.

1901, 34, 413), p-toluenesulphonyl hydrazine (1.95 p-toluenesulphonic acid (0.27 g) and ethanol (15 ml) was boiled under reflux for 72 hours. The solvent was removed under reduced pressure and the residue was purified by flash column chromatography on silica using petroleum ether, b.p. 60-80 0 C ethyl acetate as the mobile phase. Appropriate fractions were collected, combined and evaporated to give a residue which was triturated with petroleum ether, b.p. 60-80 0 C, to give a solid which was crystallized from ethanol to give 4-(1,4-dihydroindeno[l,2c]pyrazol-3-yl)phenol, m.p. 266-268 0

C.

Example 31 A mixture of 3-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenol (0.29 g), ethylene carbonate (0.11 a trace of solid sodium hydroxide (spatula tip of 20-40 mesh beads) and dry dimethylformamide (3 ml) was stirred under nitrogen and heated at 100 0 C for 45 minutes. The external temperature was raised to 140 0 C and the WO 00/27822 PCT/US99/26105 92 mixture heated at this temperature for 3.5 hours. The reaction mixture was cooled and diluted with ethyl acetate and the mixture washed with dilute aqueous sodium hydroxide solution and then with water. The organic phase was dried, filtered and evaporated to give a residue which was purified by flash column chromatography on silica using ethyl acetate as the mobile phase. Appropriate fractions were collected, combined and evaporated to give a solid with was triturated with diethyl ether and filtered to give 2-[3-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenoxy]ethanol, m.p. 203- 205 0

C.

Example 32 A mixture of 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzoic acid (0.37 g), dichloromethane (20 ml) and oxalyl chloride (0.26 ml) was stirred at 15-20C and then dimethylformamide (3 drops) was added. The mixture was stirred at 15-20 0

C

for 30 minutes and then warmed at 40 0 C for 1 hour. The solvent was removed under reduced pressure to give a solid which was dissolved in dichloromethane (20 ml) and then aniline (0.13 ml) and triethylamine (0.38 ml) were added. The mixture was stirred at ambient temperature under nitrogen for 2 hours and then washed with water, dried, filtered and evaporated to give a solid which was purified by flash column chromatography on silica to give 4-(1,4-dihydroindeno[1,2-c]pyrazol-3yl)benzanilide, m.p. 186-190 0

C.

Example 33 A mixture of 3-(1,4-dihydroindeno-[ 1,2-c]pyrazol-3-yl)phenol (2.4 g), anhydrous potassium carbonate (1.2 ethyl 2-bromoacetate (1.2 ml) and dry dimethylformamide (20 ml) was stirred at ambient temperature for 24 hours. The mixture was diluted with dichloromethane (200 ml), washed with water, then 1N sodium hydroxide solution. The organic layer was separated, dried, filtered and evaporated to give an oil which was purified by flash column chromatography on silica using ethyl acetate/petroleum ether b.p. 60-80°C (1:2 as the mobile phase).

Appropriate fractions were combined and evaporated to give ethyl 3-(1,4dihydroindeno[1,2-c]pyrazol-3-yl)phenoxyacetate, m.p. 134-136°C.

WO 00/27822 PCT/US99/26105 93 Example 34 A mixture of ethyl 3-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenoxyacetate (0.66 1M sodium hydroxide solution (4 ml) and ethanol (20 ml) was heated on a steam bath for 30 minutes. The solvent was removed under reduced pressure and the residue was stirred for 1 hour with 1M hydrochloric acid (20 ml) at ambient temperature. The mixture was filtered to give 3-(1,4-dihydroindeno[1,2-c]pyrazol-3yl)phenoxyacetic acid, m.p. 246-248 0

C.

Example In a similar manner to example 33, a mixture of 3-(1,4-dihydroindeno- [1,2-c]pyrazol-3-yl)phenol (0.20 g) was reacted with ethyl 4-bromobutyrate (0.16 g) to give ethyl 4-[3-(1,4-dihydroindeno-[1,2-c]pyrazol-3-yl)phenoxy]butyrate, m.p.

142-148 0

C.

Example 36 In a similar manner to example 34, ethyl 4-[3-(1,4-dihydroindeno- [1,2-c]pyrazol-3-yl)phenoxy]butyrate (0.36 g) was reacted with 1M sodium hydroxide solution (5 ml) in ethanol (10 ml) to give 4-[3-(1,4-dihydroindeno[1,2c]pyrazol-3-yl)phenoxy]butyric acid, m.p. 216-217°C.

Example 37 A mixture of 4-[3-(1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenoxy]butyric acid (0.24 g) was stirred in dry tetrahydrofuran (5 ml) at ambient temperature and triethylamine (0.20 ml) was added. The mixture was stirred at ambient temperature for 15 minutes then cooled in an ice bath and then methyl chloroformate (0.2 ml) was added. The mixture was stirred at ice bath temperature for 1 hour and then concentrated ammonia solution (SG 0.880, 5 ml) was added dropwise by pipette.

The mixture was stirred at 0°C for 5 minutes and then evaporated to dryness under reduced pressure. The residue was purified by flash column chromatography on silica using dichloromethane/IMS/triethylamine as the mobile phase.

Appropriate fractions were combined and evaporated to give dihydroindeno[1,2-c]pyrazol-3-yl)phenoxy]butyramide, m.p. 200-202 0

C.

WO 00/27822 PCT/US99/26105 94 Example 38 a) Chloroacetyl chloride (0.3 g) was added to a mixture of 4-(1,4dihydroindeno[1,2-c]pyrazol-3-yl)aniline (0.6 dichloromethane (20 ml) and triethylamine (0.27 g) at 0°C with stirring under nitrogen. Further triethylamine (0.37 ml) and chloroacetyl chloride (0.21ml) were added and the mixture was stirred for a further hour. The mixture was filtered and washed with water to give 4'-(1-chloroacetyl-1,4-dihydroindeno[1,2c]pyrazol-3-yl)chloroacetanilide.

b) A mixture of 4'-(1-chloroacetyl-1,4-dihydroindeno[1,2-c]pyrazol-3yl)chloroacetanilide (0.7 tetrahydrofuran (40 ml) and morpholine (0.61 g) was boiled under reflux under nitrogen for 5 hours. Potassium carbonate (0.97 g) was added and the mixture was boiled under reflux for a further hour. The mixture was poured into water and extracted with ethyl acetate to give 4'-(1,4-dihydroindeno[ ,2-c]pyrazol-3-yl)morpholinoacetanilide, m.p.

291-292 0

C.

Example 39 A mixture of 4-(1,4-dihydroindeno[1,2-c]pyrazol-yl)benzoyl chloride [(500 mg) prepared by reaction of the product of Example 8 (1.45 g) with oxalyl chloride (1.5 ml) in dichloromethane (100 ml) and DMF (8 drops) followed by evaporation of the solvent], dichloromethane (50 ml), 2-morpholinoethanol (0.22 ml) and triethylamine (0.5 ml) was stirred at ambient temperature for hours. The mixture was washed with water then with saturated sodium bicarbonate solution, then dried, filtered and evaporated to give an oil which was purified by flash column chromatography on silica using dichloromethane/IMS (10:1) as the mobile phase to give an oil which was purified again by flash column chromatography using dichloromethane/IMS (25:1) as the mobile phase to give the product 2-morpholinoethyl 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)- benzoate as a solid, m.p. 168-172 0

C.

WO 00/27822 PCT/US99/26105 Example A mixture of methyl 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl]benzoate (2.2g, Example 7) and N,N-diethylethylenediamine (7ml) was boiled under reflux for 5 hours. The mixture was cooled and stood for 16 hours at ambient temperature.

Petroleum ether, b.p. 60-80 0 C (50ml) was added and then decanted off to leave a gum which was further treated with petroleum ether as above and the residual gum dissolved in dichloromethane and purified by flash column chromatography on silica using dichloromethane/IMS/triethylamine (25:2:1) as the mobile phase to give a solid which was dissolved in ethanol (70ml) and then dry hydrogen chloride gas was passed through the solution. The precipated solid was collected by filtration, washed with ethanol and dried to give N-(2-diethylaminoethyl)-4-(1,4-dihydroindeno[l,2-c]pyrazol-3-yl)- benzamide dihydrochloride, m.p.230 0

C.

Examples 41-50 Step 1 A 1:1 mixture (by volume) of dichloromethane:methanol (lml) was added via a Gilson 215 liquid handler, to the starting enone (approx. 50mg, see Table A) in a septum sealed tube, followed by aqueous 2M sodium hydroxide solution (1 molar equivalent, see Table A) followed by 100 volumes aqueous hydrogen peroxide (1.8 molar equivalents). The resulting solutions/suspensions were then shaken at ambient temperature for 20 hours. Following LCMS analysis, further 100 volumes aqueous hydrogen peroxide (1.8 molar equivalents) was added to each reaction tube, this time manually via a plastic-tipped pipette. To those tubes containing a significant quantity of insoluble material was also added further 1:1 (by volume) dichloromethane:methanol (1ml). Shaking was then continued for a further 24 hours.

All reactions were analysed by t.l.c. and to those judged to have given incomplete reaction was added further 100 volumes aqueous hydrogen peroxide (1.8 molar equivalents) again via a plastic-tipped pipette (see those reactions indicated by x3 in Table These reaction mixtures were stirred at ambient temperature for 3 days.

WO 00/27822 WO 0027822PCT/US99/261 96 The reaction solutions/suspensions were equilibrated between dichioromethane (approx. 5mi) and water (approx. 2ml), filtering off the organic phase through an Empore®I filter cartridge and washing through with dichioromethane (approx. 2m1). The dichioromethane phases were evaporated and the residues further dried in vacuo.

TABLE A Starting enone (mg) 2N aq. NaGH (iii) 100 volumes H202 G11) 41 2-(3-Nitrobenzylidene)-1-indanone 93.7 38.3 x 3 (49.7 mg) 42 2-(4-Thiomethoxybenzylidene)-1- 94.2 38.5 x 2 __indanone (50.2 mg) 43 2-(2-Naphthylmethylene)-1-indanone 94.9 38.8 x 2 mg) 44 2-(4-Difluoromethoxybenzylidene)-lI- 89.6 36.7 x 2 indanone (51.3 mg) 2-(4-Acetamidobenzylidene)-l- 86.7 35.5 x 3 ___tetralone (50.5 mg) 46 2-(4-Bromo-2-thienylmethylene)-1- 83.4 34.1 x 2 indanone (50.9 mg) 47 2-(4-Benzyloxybenzylidene)-l- 74.5 30.5 x 2 ___tetralone (50.7 mg) 48 5,6-Dimethoxy-2-(3- 67.4 27.6 x 3 phenoxybenzylidene)-l1-indanone (50.2 mg) 49 2-[4-(5-Trifluoromethyl-2- 65.8 26.9 x 2 pyridyloxy)benzylidene] I -indanone mg) 5,6,7-Trimethoxy-2-(2,3,4- 62.4 25.5 x 3 trimethoxybenzylidene)- I -indanone mg) The step 1 products were taken on on the basis of being the corresponding 2,3-epoxyketones.

WO 00/27822 PCT/US99/26105 97 Step 2 To the products from step 1 in a septum sealed tube was added, via a Gilson 215 liquid handler, n-butanol (2ml) followed by hydrazine hydrate (2 molar equivalents based on amount of starting enone used in step 1) as a 10% solution by volume in n-butanol (see Table To each tube was finally added glacial acetic acid (2 drops) manually by syringe. The reactions were heated at 100°C with shaking for the times indicated in Table B, based on monitoring by t.l.c. analysis.

Reaction mixtures were evaporated to dryness and the residues were purified by chromatography on silica eluting with ethyl acetate, diluted where necessary with petroleum ether, b.p. 40-60 0 C. Appropriate fractions were evaporated and dried in vacuo. Where necessary, products were further purified by preparative HPLC (see Table B) to give the final pyrazoles. The products were analysed by LCMS and the conditions are given later.

Table B Reaction Details Example Volume of 10% Heating time at Further purification NH2NH2.H20 t1l 100°C of product by prep.

HPLC

41 182.5 6 h Yes 42 183.6 6 h No 43 184.8 22 h No 44 174.5 6 h No 168.8 38h Yes 46 162.4 22 h No 47 145.1 38 h No 48 131.3 22 h No 128.2 6 h No 121.6 22 h No WO 00/27822 WO 0027822PCTIUS99/26105 Yields/LCMS Example MF MWt M+ found HPLC RT Final Mass 41 C 1 6

HI

1

N

3 277 Yes 4.67 3.5 mg 42 C,- 7

H,

4

N

2 S 278 Yes 4.94 5.3 mg 43 C-)nH 1 4 N, 282 Yes 5.29 21.9 mg 44 CHI,FN,O 298 Yes 4.82 21.2 mg C19H1NO0 303 Yes 3.67 5.0 mg 46 C 14 HBrN,S 317 Yes 5.16 10.2 mg 47 C,,H, 0 NO 352 Yes 5.93 2.7 mg 48 C,) 4

H,

0

NNO

3 384 Yes 5.16 16.3 mg 49 C), 2

H,

4 FNIO 393 Yes 5.56 20.1 mg

C

2 2

H

24

N

2 0 6 42 Yes 4.25 11.9 mg Example 41 3-(3-Nitrophenyl)- 1,4-dihydroindeno[1I,2-c]pyrazole Example 42 3-(4-Thiomethoxyphenyl)- 1,4-dihydroindeno[ 1,2-c]pyrazole Example 43 3-(2-Naphthyl)-1 ,4-dihydroindeno[1 ,2-clpyrazole Example 44 3-(4-Difluoromethoxyphenyl)- 1,4-dihydroindeno[ 1,2-c]pyrazole Example 3-(4-Acetamnidophenyl)-4,5-dihydro-2H-benz[g]indazole Example 46 3-(4-Bromo-2-thienyl)-4,S-dihydroindeno[ 1,2-c]pyrazole WO 00/27822 WO 0027822PCT/US99/261 99 Example 47 3-(4-Benzyloxyphenyl)-4,5 -dihydro-2H-benz[g]indazole Example 48 6,7-Dimethoxy-3-(3-phenoxyphenyl)- 1,4-dihydroindeno- [1 ,2-c]pyrazole Example 49 -Trifluoromethyl-2-pyridyloxy)phenyl]- 1,4-dihydroindeno[ 1,2-c]pyrazole Example 6,7,8-Trimethoxy-3-(2,3 ,4-trimethoxyphenyl)- 1,4-dihydroindeno[ 1,2-c]pyrazole

LC

Column: Mobile Phase: Conditions: (gradient) Flow Rate: Wavelength Range: Injection Volume:

MS

Ionization Mass Range: 150-50 Cone Voltage: 5 HYPERSILBDSC8(100X2.lmm) 0. 1% Formic Acid MeCN (gradient see below) 10-100% MeCN in 8 minutes 100% MeCN for 1 minute 100-10% MeCN in 2 minutes (Total analysis run time 11 minutes) 1 mllmin 206-320 n 1 OJJ1l APcI +vel-ve 0Q m/z Example 51 a) A mixture of 4-hydroxy-3-(hydroxymethyl)benzaldehyde (1.08 indan-1 one (0.94 ethanol (30 ml) and SM aqueous sodium hydroxide solution (1.6 ml) was boiled under reflux for 6.5 hours. The mixture was cooled to WO 00/27822 PCT/US99/26105 100 ambient temperature and allowed to stand at that temperature for 16 hours.

The mixture was diluted with water (45 ml) and then with 5M hydrochloric acid (1.6 ml) which was added with cooling. The precipitate formed was collected by filtration, washed with water and dried to give 2-(4-hydroxy-3- (hydroxymethyl)benzylidene)-1-indan-one, m.p. 194-195 C.

b) A mixture of the product from a) (0.85 p-toluenesulphonylhydrazine (0.9 p-toluenesulphonic acid (0.12 g) and ethanol (15 ml) was boiled under reflux for 4.5 hours. The solvent was removed under reduced pressure at ambient temperature and the residue was partitioned between dichloromethane and aqueous sodium bicarbonate solution. The organic layer was separated, washed with water and the dichloromethane was removed by pipette from a gum. The gum was purified by flash column chromatography on silica using 10% and the 20% IMS in dichloromethane as a mobile phase. Appropriate factions were collected, combined and evaporated to give a solid which was triturated with diethyl ether to give 4- (1,4-dihydroindeno[1,2-c]pyrazol-3-yl)-2-hydroxymethyl)phenol, m.p. >300°C.

Examples 52-55 The following examples were prepared in a similar manner to Example 51 by reacting indan-1-one with the appropriate aldehyde; Example 52 2-Methoxy-5-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenol, m.p. 189-199 0 C, prepared from 3-hydroxy-4-methoxybenzaldehyde.

Example 53 2-Chloro-4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenol, m.p. 235-236 0

C,

prepared from 3-chloro-4-hydroxybenzaldehyde.

WO 00/27822 PCT/US99/26105 101 Example 54 2-Methoxy-4-(1,4-dihydroindeno [1,2-c]pyrazol-3-yl)phenol, m.p. 243-244°C, prepared from 4-hydroxy-3-methoxybenzaldehyde.

Example 3-Chloro-4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenol, m.p. 231-233 C, prepared from 2-chloro-4-hydroxybenzaldehyde.

Example 56 A mixture of 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenol (0.28 g, Example 30), potassium carbonate (0.16 g) and 2-bromoacetamide (0.16 was stirred together at ambient temperature in dry dimethyl formamide (5 ml) for 4 days and then allowed to stand at ambient temperature for 9 days. The mixture was diluted with dichloromethane (50 ml) and washed with 1M aqueous sodium hydroxide solution and then with water. Some insoluble material which remained throughout the extractions was collected by filtration. The solid was purified by flash column chromatography on silica using ethyl acetate as the mobile phase.

Appropriate fractions were collected, combined and evaporated to give dihydroindeno[1,2-c]pyrazol-3-yl)phenoxy]acetamide, m.p. 247-248°C.

Example 57 A mixture of 4'-(1-chloroacetyl-1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)chloroacetanilide (1.0 g) in THF (40 ml), diethylamine (0.73 g) and potassium carbonate (1.38 g) was boiled under reflux for 6 hours and then cooled to 20 0 C and stood at this temperature for 16 hours. The mixture was poured into water/ethyl acetate ml of each) and the organic layer was separated, dried and evaporated to give an oily solid which was purified by flash column chromatography on silica using methanol in dichloromethane as the mobile phase and then rechromatographed using ethyl acetate as the mobile phase followed-by 5% methanol in dichloromethane.

Appropriate fractions were collected, combined and evaporated to give dihydroindeno[1,2-c]pyrazol-3-yl)diethylamino-acetanilide, m.p. 99-100 0

C.

WO 00/27822 PCT/US99/26105 102 Example 58 a) A mixture of 4-cyanophenacyl bromide (3.0 methyl thiosalicylate (2.25 g), sodium methoxide (1.52 g) and ethanol (100 ml) was boiled under reflux for hours. The mixture was cooled and diluted with 2M hydrochloric acid (100 ml). A precipitate was collected by filtration and recrystallized from ethanol to give 2-(4-cyanobenzoyl)-3-hydroxybenzo[b]thiophene.

b) Hydrazine hydrate (5 ml) was added to a solution of the product from a) in ethanol (100 ml) containing glacial acetic acid (1 drop) over 4A molecular sieves (5 The mixture was boiled under reflux for 24 hours and then filtered. The filtrate was concentrated under reduced pressure and the residue obtained was purified by flash column chromatography on silica using diethyl ether/petroleum ether, b.p. 60-80 0 C as the mobile phase to give a solid which was recrystallized from ethanol/water to give 4-(1H-[l]benzothieno[3,2c]pyrazol-3-yl)benzamide, m.p. 290-292 0 C (with decomposition).

Example 59 In a similar manner to Example 58, a mixture of methyl thiosalicylate (2.76 g), 4-nitrophenacyl bromide (4.0 sodium methoxide (1.94 g) and ethanol (200 ml) gave 2-(4-nitrobenzoyl)-3-hydroxybenzo[b]thiophene of which 2.25 g was reacted with hydrazine hydrate (6 ml) in a similar manner to Example 58, to give 3-(4aminophenyl)-1H-[1]benzothieno [3,2-c]pyrazole, m.p. 113-115°C.

Example In a similar manner to Example 58, 4-methoxyphenacyl bromide (0.81 g) was reacted with methylthiosalicylate (5.0 g) in sodium methoxide and methanol to give 2- (4-methoxybenzoyl)-3-hydroxy[b]thiophene which was reacted with hydrazine hydrate to give 3-(4-methoxyphenyl)-1H-benzothieno[3,2-c]pyrazole, m.p. 185-187 0

C.

Example 61 Boron tribromide (3.0 ml of a 1.OM solution in dichloromethane) was added to a solution of the product from Example 60 (210 mg) in dichloromethane (10 ml) WO 00/27822 PCT/US99/26105 103 at -78 0 C under nitrogen with stirring. The mixture was allowed to warm to ambient temperature slowly, and after stirring at this temperature for 2 days the mixture was quenched by the addition of aqueous ammonium chloride solution. The organic layer was separated, dried and evaporated to give a residue which was purified by flash column chromatography on silica using dichloromethane and then increasing amounts of methanol, up to 15% of methanol, in dichloromethane as the mobile phase. Appropriate fractions were collected, combined and evaporated to give 3-(4hydroxyphenyl)-lH-[1]benzothieno[3,2-c]pyrazole, m.p. 137-139 0

C.

Example 62 (2.5 g) was reacted with benzoyl chloride (2.63 g) in dichloromethane and triethylamine to give 5-benzamidoindan-l-one which was reacted with benzaldehyde in a similar manner to Example 7a) to give N-(2which was reacted with hydrogen peroxide in a similar manner to Example 7 b) to give an intermediate which was reacted with hydrazine hydrate in a similar manner to Example 7 c) to give N -phenyl-1,4dihydroindeno[l,2-c]pyrazol-6-yl)benzamide, m.p. 271 after chromatography of the reaction mixture.

Example 63 4-(1,4-Dihydroindeno[1,2-c]pyrazol-3-yl)phenol was stirred with water ml) containing 2M aqueous sodium hydroxide solution (0.41 ml). The suspension was warmed gently and then stirred at ambient temperature for 1.5 hours and then ethanol (20 ml) was added to give almost complete solution. The solvent was removed under reduced pressure and the residue was dried under vacuum. The solid was triturated with diethyl ether (10 ml) and filtered to give 4-(1,4dihydroindeno[1,2-c]pyrazol-3-yl)phenol sodium salt, m.p. >300 0

C.

The compounds shown in Tables 1, 2 and 3 were prepared in a similar manner to Example 7b and 7c. Alternatively these compounds are prepared in a similar manner to Example WO 00/27822 PCT/US99/26105 104 Example 64 A mixture of methyl 4-(1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzoate (100 mg) and N-(2-aminoethyl)morpholine (0.5 ml) was stirred and heated at 150 0

C

for 5 hours. The mixture was cooled and washed with petroleum ether, b.p. 40-60 0

C

(20 ml). The mixture was dissolved in a mixture of dichloromethane/IMS/triethylamine (25:2:1) and purified by flash column chromatography on silica using the same solvent mixture as the mobile phase. Appropriate fractions were collected, combined and evaporated to give a gum which was dissolved in dichloromethane, washed with water, then dried, filtered and evaporated to give a solid which was triturated with ether and filtered to give N-(2-morpholinoethyl)-4-(1,4dihydroindeno[1,2-c]pyrazol-3-yl)benzamide monohydrate, m.p. 210-214°C.

Example In a similar manner to Example 7a, indan-1-one was reacted with 4cyanobenzaldehyde and the intermediate product obtained was reacted with hydrogen peroxide in a similar manner to Example 7b to give an intermediate spiro compound which was reacted with hydrazine hydrate in a similar manner to Example 7c to give 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzonitrile, m.p. 245 0

C.

Example 66 6-Methoxy-2-(4-thiomethoxybenzylidene)-1-tetralone was reacted with hydrogen peroxide and then with hydrazine hydrate following the general procedure described for examples 41-50 to give 7-methoxy-3-(4-methylsulphonylphenyl)-4,5dihydro-2H-benz[g]indazole 2 0 3 S MWt 354, M' found, percentage purity by HPLC 66%).

Example 67 3-Phenylindeno[1,2-c]pyrazol-4-(1l)-one (2.4g) was suspended in toluene ml) under nitrogen and trimethyl aluminium (2.2 ml of a 2M solution in heptane) was added at 20 0 C with stirring. The mixture was heated at 90 0 C for 16 hours, then cooled to ambient temperature and poured on to crushed ice (approx. 600 ml) containing WO 00/27822 PCT/US99/26105 105 concentrated hydrochloric acid (30 ml). The precipitated solid was collected by filtration and recrystallized from ethyl acetate with hot filtering to give 4-methyl-3phenyl-1,4-dihydroindeno[ 1,2-c]pyrazol-4-ol, m.p.154-155 C.

Example 68 Dihydroxy 4-(4H-indeno-1,.2-cl-pvrazol-3-vl)phenvlborane.

(HO)2B

N

H

A solution of 3-(4-bromophenyl)-4H-indeno[1,2-c]pyrazole (2.0g, 6.43 mmol) in dry THF (50 ml) was added to a stirred suspension of potassium hydride (35% wt dispersion in mineral oil, 0.365g, 6.75mmol) in dry THF (50 ml) in an atmosphere of nitrogen. After 10 mins the mixture was cooled to about -78 0 C and tert-butyl lithium (1.5M solution in pentane, 8.78 ml, 13.18mmol) was added and the mixture stirred for about 15 mins. A solution of tri-isopropylborate in THF (1.56ml, 6.75mmol) was added to the red solution and the mixture was allowed to warm up to room temp and stirred overnight. Hydrochloric acid (1M, 150ml) was added and the solution stirred for about 30 mins and extracted with ether (3x200ml). The combined ether extracts were then extracted with sodium hydroxide (3x100ml). The combined alkaline extracts were acidified with hydrochloric acid (2M) and the precipitated off solid was filtered and dried at the pump to give dihydroxy 4-(4Hindeno-[1,2-c]pyrazol-3-yl)phenylborane (602 mg, Mpt >300 0

C

Example 69 4-(1H-l ]Benzothienor3,2-clpvrazol-3-vl)benzaldehvde.

o

H

WO 00/27822 PCT/US99/26105 106 A solution of 3-(4-bromophenyl)-1H-[1]benzothieno[3,2-c]pyrazole (4.0g, 12.2 mmol) in dry THF (200ml) was added dropwise to a stirred suspension of potassium hydride (35% wt dispersion in mineral oil, 1.53g 13.4 mmol) in an atmosphere of nitrogen, maintaining the temp at about 0°C. After complete addition, the reaction mixture was stirred for about 15 mins at about 0°C then cooled to about -78 0 C. Tertbutyl lithium (1.5M solution in pentane, 17ml, 25.62 mmol) was then added dropwise and the mixture stirred for about 45 mins. Dry DMF (61 mmol) was then added dropwise, maintaining the temp at about -78 0 C. The resulting mixture was stirred at this temp for about 1 hr, allowed to warm to room temp and stirred overnight. The reaction mixture was quenched by careful addition of hydrochloric acid (1M, 200ml) and the two layers separated. The aqueous layer was extracted with ether (2x 200ml). The combined extracts and the organic layer were washed with saturated sodium bicarbonate (1x200ml), water (3x 200ml) dried (MgSO 4 filtered and the solvent was removed to give a waxy red solid (1.3g) which was purified by flash chromatography (eluting with 20% ethyl acetate/80% 60-80 petroleum ether) to give 1H-[ 1]benzothieno[3,2-c]pyrazol-3-yl)benzaldehyde (200mg, Mpt 261-3°C Example 70 4-(1H-r[1Benzothienor3,2-clpvrazol-3-vl)-N-F3-(imidazol-1vl)propyl]benzvlamine trihvdrochloride.

N

H

N

H ,3HCI A solution of 4-(1H-[1]benzothieno[3,2-c]pyrazol-3-yl)benzaldehyde (0.1g 0.36mmol), 3-(imidazol-l-yl)propylamine (0.058g, 0.47mmol) and glacial acetic acid (0.03ml, 0.47mmol) in 1,2-dichloroethane was stirred at room temp for about 1 hr. Solid sodium triacetoxyborohydride (0.084g, 0.4 mmol) was then added in one portion and the resulting mixture stirred for about 16 hrs at room temp. An WO 00/27822 PCT/US99/26105 107 additional portion of sodium triacetoxyborohydride (0.084g, 0.4 mmol) was added and the mixture stirred for another 24 hrs. The reaction mixture was poured onto a solution of saturated sodium bicarbonate (20ml) and stirred for about 30 mins, the layers separated and the aqueous layer extracted with dichloromethane (2x50ml).

The combined extracts and organic layer were washed with water (1x25ml), dried (MgSO 4 filtered and concentrated in vacuo to give a waxy solid which was taken up in ethanol. Two drops of hydrochloric acid (conc) were added, the solution cooled and scratched to give a solid which was removed by filtration, dried in vacuo to give 4-(1H-[1 ]benzothieno[3,2-c]pyrazol-3-yl)-N-[3-(imidazol-1-yl)propyl] benzylamine trihydrochloride (0.09g, 50%) Mpt 206-208°C.

Example 71 Methyl 4-(4-oxo-1.4-dihydroindenor 1.2-clpyrazol-3-vl)benzoate

H

y^N 0 0 A mixture of methyl 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzoate (6.3g, Example sodium bismuthate (31 glacial acetic acid (180ml) and water was boiled under reflux for about 3.5 hours. A further amount of sodium bismuthate (33g) was added and the mixture boiled under reflux for about 24 hours, then cooled.

The pH of the reaction mixture was adjusted to 8 9 by addition of saturated sodium bicarbonate solution. The reaction mixture was filtered through a celite pad and the residue solid washed with ethyl acetate. The organic layer was separated, dried, filtered and evaporated to give a brown solid. The solid was boiled up in methanol cooled and the solid was filtered off, then dried to give methyl 4-(4-oxo-1,4dihydroindeno[1,2-c]pyrazol-3-yl)benzoate, m.p. dec. 235°C.

WO 00/27822PCIS9210 PCTIUS99/26105 108 Example 72 4-(1 .4-dihydroindenor 1 2-clpvrazol-3-yl)benzamide oxime.

0 NH2

OH

A mixture of 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzonitrile (730mg, Example 65), hydroxylamine hydrochloride sodium carbonate (0.8g) and ethanol (30m1) was boiled under reflux for about 16 hours. The solvent was removed under reduced pressure at about 50'C and the residue stirred with water (70m1). The mixture was extracted with ethyl acetate and the combined organic phases dried, filtered and evaporated to give a solid. The solid was purified by flash column chromatography on silica using ethyl acetate as eluant to give a solid which was washed with diethyl ether and dried to give 4-(l,4-dihydroindeno[1,2-c]pyrazol- 3-yl)benzamide oxime, 300*C.

Example 73 3- f4-r'2-diethvlaminoethyl)aminomethynlphenvp, -1 .4-dihydroindeno 1 2-cljpvrazole trihydrochloride

N

A mixture of N-(2-diethylaminoethyl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)benzamide (2.75g, Example 29) and dry tetrahydrofiran (lO0ml) was stirred at room temperature under nitrogen and lithium alumniniumn hydride (3.4g) was added WO 00/27822 PCTIUS99/26105 109 portionwise. The reaction mixture was then boiled under reflux for about 2.5 hours, then cooled in an ice/water bath. Ethyl acetate (100ml) and water (100ml) were added. Then the organic phase was separated off, washed with brine (25ml), dried, filtered and evaporated to give a yellow oil. The oil was purified by flash column chromatography on silica using ethyl acetate/ ethanol/ triethylamine (7 2 1) as eluant to give a pale yellow gum. The gum was dissolved in warm ethanol hydrochloric acid (conc, 0.6ml) was added and the solvent then removed under reduced pressure. The gummy solid residue was boiled up with ethanol (10ml) and cooled in ice. The precipitated solid was collected by filtration, washed with ethanol and dried to give 3- {4-[(2-diethylaminoethyl)aminomethyl]phenyl}-1,4dihydroindeno[1,2-c]pyrazole trihydrochloride, m.p. 225 0

C.

Example 74 N-[4-(1,4-Dihvdroindeno[1.2-cpyvrazol-3-vl)phenvll benzenesulphonamide.

H

N-N

,S

a) 4'-(1,4-Dihydroindeno[l,2-c]pyrazol-3-yl)aniline (1 g) was dissolved in dichloromethane (30 ml) and triethylamine (0.62 ml) was added. The reaction mixture was then cooled to about 0°C and benzenesulphonyl chloride (0.57 ml) added dropwise over 5 minutes. The reaction mixture was then warmed to ambient temperature and stirred for about 2 hours. A further addition of benzenesulphonyl chloride (0.57 ml) and triethylamine (0.62 ml) was made and stirring continued for about another 4 hours at ambient temperature. Diethyl ether (80 ml) and water ml) was then added. A solid precipitated which was filtered and washed with an aqueous solution of sodium bicarbonate (40 ml) and diethyl ether (40 ml). The solid was further purified by flash chromatography over silica using dichloromethane as WO 00/27822 PCT/US99/26105 110 eluant. Early fractions were combined and solvent removed in vacuo to give N-[4- (1 -benzenesulphonyl- ,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenyl] benzenesulphonamide as a brown solid (0.35 g) m.p. 251-252 0 C, later fractions gave as a brown solid (0.76 g) m.p. 267-268 0

C.

b) N-[4-(1-benzenesulphonyl-1,4-dihydroindeno[l1,2-c]pyrazol-3- yl) phenyl]benzenesulphonamide (0.56 g) was suspended in methanol (40 ml) and a 2N aqueous solution of sodium hydroxide (5.3 ml) added at ambient temperature. A clear solution was obtained. The reaction mixture was stirred at ambient temperature for about 20 minutes and then poured onto a 2N aqueous solution of hydrochloric acid (75 ml). A colourless solid precipitated, which was collected by filtration (0.42 The solid was partitioned between an aqueous solution of sodium bicarbonate ml) and ethyl acetate (25 ml), and stirred for about 30 minutes at ambient temperature. It was then collected by filtration and dried in vacuo at about 60 0 C to give 4 -dihydroindeno[1,2-c]pyrazol-3-yl)phenyl]benzenesulphonamide as a colourless solid (0.14 g) m.p. 286-288 0

C.

Example 75 N-(2-Morpholinoethvl)-4'-dihvdroindeno[l.2-c]pvrazol-3-vlaniline dihydrochloride

H

N'N

NN

H

4'-(1,4-Dihydroindeno[1, 2 -c]pyrazol-3-yl)morpholinoacetamide (1.5 g, Example 38) was dissolved in tetrahydrofuran (80 ml) at ambient temperature under nitrogen and lithium aluminium hydride (0.6 g) was added portionwise over 10 minutes. The mixture was stirred at ambient temperature for about 6 hours, then poured onto a saturated aqueous solution of sodium sulphate (75 ml) and extracted with diethyl ether (2 x 75.ml). The combined organic extracts were dried (Na 2 SO) and evaporated in vacuo to give a yellow oil (2.24 g) which was purified by flash chromatography over silica using a gradient of a 1:20 to 1:4 mixture of methanol and WO 00/27822 PCT/US99/26105 111 dichloromethane as eluant. Appropriate fractions were combined and evaporated in vacuo to give a yellow solid (0.83 The solid was dissolved in ethanol (10 ml) and concentrated hydrochloric acid (0.5 ml) was added. A colourless solid precipitated which was collected by filtration to give N-(2-morpholinoethyl)-4'dihydroindeno[1,2-c]pyrazol-3-ylaniline dihydrochloride (0.94 g) m.p. 284-285 0

C.

Example 76 N-(1.4-Dihvdroindeno 1.2-c]pvrazol-6-vl)-2-morpholinoacetamide

H

NN

0 O

ON

H

a) 3-Phenyl-l,4-dihydroindeno[1,2-c]pyrazol-6-ylamine (2.0 g) was dissolved in dichloromethane (70 ml) and triethylamine (2.3 ml) was added. The resultant mixture was cooled to about 0°C and chloroacetyl chloride (1.3 ml) added. The reaction mixture was then stirred at ambient temperature for 10 hours under nitrogen. A colourless solid precipitated which was collected by filtration and dried in vacuo at about 60 0 C to give N-(1-chloroacetyl-3-phenyl-1,4-dihydroindeno[1,2c]pyrazol-6-yl)-2-chloroacetamide (2.04 g) used in the next stage without further purification.

b) N-(1-chloroacetyl-3-phenyl- ,4-dihydroindeno[1,2-c]pyrazol-6-yl)-2chloroacetamide (1.0 g) was dissolved in tetrahydrofuran (30 ml) and morpholine (0.87 ml) added. The mixture was then heated under reflux for about 90 minutes, cooled to ambient temperature and poured onto water (100 ml). A solid precipitated which was collected by filtration and dried in vacuo at about 60°C to give N-(1,4dihydroindeno[1,2-c]pyrazol-6-yl)-2-morpholinoacetamide as a brown solid (0.87 g) m.p. 141-148 0

C.

WO 00/27822 PCT/US99/26105 112 Example 77 N-(2-Morpholinoethvl)-3-phenvl-1,4-dihvdroindeno[1.2-cvpyrazol-6ylamine trihydrochloride

H

N,N

NNN

H

N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)-2-morpholinoacetamide (0.6 g) was dissolved in tetrahydrofuran (40 ml) at ambient temperature under nitrogen and lithium aluminium hydride (0.24 g) added portionwise over about 10 minutes. The mixture was stirred at ambient temperature for about 24 hours. A further addition of lithium aluminium hydride (0.24 g) was added and the mixture was stirred at ambient temperature for about another 24 hours. It was then poured onto a saturated aqueous solution of sodium sulphate (30 ml) and extracted with diethyl ether (2 x ml). The combined organic extracts were combined, dried (Na 2

SO

4 and evaporated in vacuo to give a yellow oil which was purified by flash chromatography over silica using a 1:20 mixture of methanol and dichloromethane as eluant. Appropriate fractions were combined and evaporated in vacuo to give a yellow solid. The solid was dissolved in ethanol (5 ml) and concentrated hydrochloric acid (10 drops) was added. A colourless solid precipitated was collected by filtration to give N-(2morpholinoethyl)-3-phenyl-1,4-dihydroindeno[1,2-c]pyrazol-6-ylamine trihydrochloride salt (0.18 g) m.p. 180-182 0

C.

Example 78 4'-(l-Acetyl-l 4-dihydroindenor1,2-c]pvrazol-3-vl)acetanilide

H

NN

H

WO 00/27822 PCT/US99/26105 113 a) 4 4 -Dihydroindeno[1,2-c]pyrazol-3-yl)aniline (1 g) was dissolved in dichloromethane (40 ml) and triethylamine (1.14 ml) was added followed by acetic anhydride (0.85 ml). The reaction mixture was then stirred at ambient temperature for 2 hours under nitrogen. A colourless solid precipitated which was collected by filtration and washed with water and dried in vacuo at about 60°C to give acetyl-l,4-dihydroindeno[1,2-c]pyrazol-3-yl)acetanilide as a colourless solid (0.84 g) m.p. 245-246 0

C.

b) 4'-(1-acetyl-l,4-dihydroindeno[1,2-c]pyrazol-3-yl)acetanilide (1.0 g) was suspended in methanol (40 ml) and a 2N aqueous solution of sodium hydroxide ml) added. The mixture was stirred at ambient temperature for about 20 minutes and then poured onto an aqueous solution of 2N hydrochloric acid (75 ml), a colourless solid precipitated which was collected by filtration and dried in vacuo at about 60 0

C

to give 4'-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)acetanilide dihydrochloride (0.96 g) m.p. 295-304 0

C.

Example 79 3 -F4-(2-morpholinoethox)phenvl] -14-dihydroindeno[ 1,2-clpvrazole

H

N-

N

ro A mixture of 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenol (0.76g, Example 4 -(2-hydroxyethyl)morpholine (0.85g), triphenylphosphine (1.77g) and dichloromethane (20ml) were stirred together with ice/water bath cooling and diethylazodicarboxylate (1.16g) was added dropwise. The resultant mixture was allowed to warm to room temperature and stirred for about 24 hours. The solvent was evaporated off under reduced pressure and the residue purified by flash column chromatography on silica using ethyl acetate/ethanol to give a solid. The solid was triturated with diethyl ether (10ml) and the solid filtered, washed with WO 00/27822 WO 0027822PCTIUS99/26105 114 diethyl ether and dried at about 65'C to give 3-14-(2-morpholinoethoxy)phenyl]-1,4dihydroindeno[1 ,2-c]pyrazole, m.p. 1 75-6'C.

H

R6 R 5 II N

R

R4 X R I R3 Preparation Melt Point EX R2 X R3,R4,R5,R6 Comments 00 4-(Ph)PHENYL CH, H Prep as Ex 51 245-6 81 4-(OPh)PHENYL OH 2 H Prep as Ex 7 195-6 82 4-(SO 2 Me)PHENYL (OH 2 2 R4 MeO Prep as Ex 66 83 3-(CO 2 H),4-(OH)PHENYL OH 2 H Prep as Ex 51 283-4 84 3-(N0 2 ),4-(OH)PHENYL OH 2 H Prep as Ex 51 250-2 3-(OCH 2 00NH 2 )PHENYL OH 2 H Prep as Ex 56 246-8 86 4-(OCH 2 Ph)PHENYL OH 2 R4,R5 (MeO) 2 Prep as Ex 51 110-4 87 14-(OCH 2 Ph)PHENYL OH 2 IR5 MeO Prep as Ex 51 228-31 88 4-(OH)PHENYL OH 2 R4, R5 (MeD) 2 Prep as Ex 13 250-4 89 3-(CONH(CH 2 2 MOR),4- HHPrpaEx416(dc (OH))PHENYL OHHPrpaEx416(d) 3-(OONH(0H 2 2 NEt 2 (OH)PHENYL OH 2 H Prep as Ex 40 210 (dec) 91 4-(Br)PHENYL OH 2 H Prep as Ex 7 277-9 92 4-(OH)PHENYL OH 2 R4 MeD Prep as Ex 13 256-60 93 4-(CONH(CH 2 3 NEt 2 H rpa x3 +8 PHENYLOHHPrpaEx3M38 94 4-(OONH(0H 2 2 OMe) H rpa x3 +3 PHENYLOHHPrpaEx3M+3 4-(OONH(4-NO 2 Ph)) H rpa x3 +9 PHENYLOHHPrpaEx3M36 96 4-(OH)PHENYL OH 2 R5 =MeD Prep as Ex 51 237-40 97 4-(CONH(0H 2 2 NEt 2 C2 R H PrpaEx9214 PHENYL O 2 R H rpa x2 1- 98 4-(Br)PHENYL S H Prep as Ex 1-6 287-9* 99 4-(OH)PHENYL OH 2 R3 O(OH 2 2 QMe Prep as Ex 51 197-8 100 4-(SO 2

NH(OH

2 2 MOR) H Prep as Ex 7 218-20 PHENYL OHH 11 4-(SO 2 NH(0H 2 2 OMe) 11PHENYL C H 2 H Prep as Ex 7 184-6 102 P-(HNYL4-H OH 2 H Prep as Ex 51 183-7 103 4-(OH)PHENYL OH 2 R3=O(0H 2 )2MOR Prep as Ex 51 280 (dec) 104 4-(SO 2 NH(0H 2 2 NEt 2 H Prep as Ex 7 192-6 PHENYL OHH 4-(OH 2 C0NH 2

OH

2 R3=0(H 2 2 OMe Prep as Ex 56 1234-6 WO 00/27822 WO 0027822PCT1US99/261 PHENYL 106 4-(OH)PHENYL CH 2 R4=(OH), Prep as Ex 51 96-100 R5=0(CH 2 2 OMe 107 4-(OH)PHENYL CH 2 R4=O(CH 2 2 OMe Prep as Ex 51 75-81 108 4-(CONH(CH 2 2 NHEt) HHPrpaEx423 PHENYLCHHPrpaEx423 109 4-(CONHCH 2 HHPrpaEx422- 1 PYRR)PHENYL CHHPrpaEx4226 110 4-(OCH 2 00NH 2 )PHENYL CH 2 R3 OH Prep as Ex 51 220 ill 4-(OCH 2

CO

2 H)PHENYL CH 2 H Prep as Ex 34 250-3 Exam~le 112 3-f2-(2H- 1.2,3,4-Tetraazol-5 -yl)-4-pvridyll-4. 5-dihydro-2Hbenzorizlindazole 4-(4,5-Dihydro-211-benzo [g]indazol-3 -yl)-2-pyridinecarbonitrile 0.l184nimoL) was dissolved in 5mL, of THF. To this was added 900mg (Il5eq) of tributyltinazide. The reaction mixture was heated for about 24 hours and the progress of the reaction was followed by HPLC. Some starting material still remained at this point; the reaction was allowed to cool to room temperature. The solvent was removed under reduced pressure. The residue was dissolved in dichioromethane (5mL) and to this was added excess IN HCl A precipitate formed which was filtered and dried to give 33mg of a white solid. HPLC/MS confirmed product formation: MH+ 316.2, retention time: 1 .73min EXPERIMENTALS FOR EXAMPLES 113-159 Tricyclic pyrazoles, ureas and carbamates.

LC conditions (analytical run): Column: PECOSPHERE, CLS, 3gm, 33x4.6mm Gradient: From 100% pH 4.5, 50m.M NHlOAc/H 2 0 to 100% CH 3 CN in minutes Flow rate: 3.5 mL/min LC/MS purification conditions: Column: Hypersil®BDS, C 18, 100~x21.2 mmn Gradient: Generally from 100% pH 4.5, 50mM NHOAc/H 2 0 to 100% CH 3

CN

in 8.5 minutes but varied depending upon required separation.

WO 00/27822 PCTIUS99/26105 116 Flow rate: 25 mL/min Melting points: Were recorded on an Electrothermal 9100 melting point microscope in open capillaries and are uncorrected.

IR:

IR spectra were recorded on a Hewlett Packard Nicolete Impact 400 FT-IR spectrophotometer using KBr pellets. Only the strongest and most significant bands are reported.

N N OH 1 0 2

N

q 3 4 -Isocvanatophenvl)-1,4-dihvdroindenor1,2-cl]prazole, 2: To a solution of 4- (1, 4 -dihydroindeno[1,2-c]pyrazol-3-yl)benzoic acid 1 (5.8 g, 20.99 mmol) in DMF (200 mL) were added diphenylphosphoryl azide (5.22 mL, 23.09 mmol) and Et 3

N

(3.35 mL, 23.09 mmol). The reaction mixture was heated to about 80 °C for about 4 h, until no more N 2 gas evolution was observed. The off-white precipitation was filtered off and washed with EtOAc (3x5 mL) and EtO2 (3x5 mL). The solid was dried under vacuum to give quantitative yield of 2 that was used in the next step without further purification. Mp 338-339 IR (KBr) 2132, 1723,1582,1520 WO 00/27822 PCT/US99/26105 117 Examples 113-121 General procedure for O-substituted N-[4-(1,4-dihydroindeno[1,2-c]pyrazol-3yl)phenyl]carbamates: To a solution of an appropriate alcohol in THF was added NaH (12 eq.) and the reaction mixture was stirred for about 45 min. at about 20 °C.

Solid 3-(4-isocyanatophenyl)-1,4-dihydroindeno[1,2-c]pyrazole, 2 was added. The reaction mixture was stirred for about 3 hours at about 45 2 M HCI was added until pH 7 and the crude material was extracted into EtOAc. Purification by flash chromatography (CH 2

CI

2 /MeOH) gave the pure carbamate 3.

Example 113: 2-(Diethylamino)ethyl N-[4-(1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl] carbamate: To a solution of N,N-diethyl ethanol amine (0.368 mL, 2.75 mmol) in THF (5 mL) was added NaH (60 in mineral oil, 113 mg, 2.75 mmol). The reaction mixture was stirred at about 20 °C for about 1 hour then solid 3-(4-isocyanatophenyl)-1,4dihydroindeno[1,2-c]pyrazole, 2 (58 mg, 0.22 mmol) was added. The reaction mixture was heated to about 45 °C for about 3 hours. The reaction mixture was quenched by addition of H 2 0 and the product was extracted into EtOAc. The crude material was purified by flash chromatography on SiO 2 (MeOH/CH 2 ,C1 10/90) to give 51 mg of pure product. LC/MS 391 LC retention time 2.68 minutes.

Example 114: 2-Morpholinoethyl N-[4-(1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]carbamate: 56 mg of pure product was isolated. LC/MS 405 LC retention time 2.69 minutes.

Example 115: 3-(Dibenzylamino)propyl N-[4-(1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl] carbamate: 34 mg of pure product was isolated. LC/MS 530 LC retention time 4.18 minutes.

WO 00/27822 WO 0027822PCTIUS99/26105 118 ExamRle 116: 2-[Ethyl(2-hydroxyethyl)arnino] ethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)phenyl]carbamate acetate salt: 19 mg of pure product was isolated. LC/MS 407 LC retention time 2.65 minutes.

Example 117: 2-[[2-(Dimethylainino)ethyl](methyl)amino]ethyl 1,4-dihydroindeno[ 1,2clpyrazol-3-yl)phenyl]carbamate acetate salt: 32 mg of pure product was isolated. LC/MS 420 LC retention time 2.80 minutes.

Example 118: 1 -Methyl-2-propoxyethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)phenyllcarbamate: 42 mg of pure product was isolated. LCIMS 392 LC retention time 3.60 minutes.

Example 119: I-Methyltetrahydro-1H-2-pyrrolyl)ethyl ,4-dihydroindeno[ 1,2-c]pyrazol-3yl)phenyl]carbamate: 4 mg (7 of pure product was isolated. LC/MS 403 LC retention time 2.81 minutes.

Example 120: 2-[2-(Dimethylamino)ethoxy~ethyl 1,4-dihydroindeno[1 ,2-c]pyrazol-3yl)phenyl]carbamate acetate salt: 32 mg of pure product was isolated. LC/MS 407 LC retention time 2.62 minutes.

Example 121: 2-(Diethylamino)-l1-methylethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3yl)phenyl]carbamate acetate salt: 7 mg of pure product was isolated. LC/MS 405 LC retention time 2.84 minutes.

WO 00/27822 WO 0027822PCT/US99/26105 119 N I N N I 2~ N 4 N'I, NRIR2 Examjles 122-159 General procedures for N'-substituted 1,4-dihydroindeno [1 ,2-c]pyrazol-3yl)phenyl]ureas 4: To a suspension of 3-(4-isocyanatophenyl)-1,4dihydroindeno[1,2-c]pyrazole, 2, in toluene was added Et 3 N (5 eq.) and an appropriate amine (5 The reaction mixture was stirred at about 95 'C for 0.5 to hours. The solid was filtered off and washed with EtOAc and Et,O and dried in vacuum to give the desired urea 4.

Example 122: N-[2-(Diethylamino)ethyl] ,4-dihydroindeno[ 1,2-c]pyrazol-3yl)phenyl]urea: A suspension of of 3-(4-isocyanatophenyl)- 1,4-dihydroindeno[ 1,2clpyrazole, 2 (75mg, 0.274 mmol), Et 3 N (0.191 mL, 1.37 mmol), N,Ndiethylethylenediamine 112 mL, 1.37 mmol) in toluene (3 mL) was heated to about 95 'C for about 3 hours. The white solid was filtered off and washed with EtOAc and dried under vacuum to afford 61 mng of pure product. LC/MS 390 LC retention time 2.76 minutes.

Example 123: ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N'-(2-morpholinoethyl)urea 84 mg of pure product was isolated. LC/MS 404 LC retention time 2.50 minutes.

WO 00/27822 WO 0027822PCT/US99/26105 120 Example 124: NI 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenyl]- 1 -piperidinecarboxamide 32 mg of pure product was isolated. LC/MS 359 LC retention time 3.36 minutes.

Examnle 125: 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-A'-[2-(dimethylamino)- 1methylethyllurea: 57 mg of pure product was isolated. LC/MS 376 LC retention time 2.72 minutes.

Example 126: 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-AP-tetrahydro-2furanylmethylurea: 57 mg of pure product was isolated. LC/MS 375 LC retention time 2.98 minutes.

Examnle 127: 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N-(2-fiirylmethyl)urea: 66 mg of pure product was isolated. LC/MS 371 LC retention time 3.34 minutes.

Examnle 128: 1,3-Benzodioxol-5-yhnethyl)-N-[4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3yl)phenyl]urea: 75 mg of pure product was isolated. LC/MS 425 LC retention time 3.45 minutes.

Example 129: N-C yclobutyl-N'-[4-( 1,4-dihydroindeno [I,2-c]pyrazol-3-yl)phenyl]: 60 mg of pure product was isolated. LC/MS 345 LC retention time 3.28 minutes.

WO 00/27822 WO 0027822PCTIUS99/26 105 121 Examle 130: 1,4-Dihydroindeno [1 2 -c]pyrazol- 3 -yl)phenyll-AP-(2-piperidinoethyl)urea urea: 64 mg of pure product was isolated. LC/MS 402 LC retention time 2.84 minutes.

Example 131: N-Benzyl-N'-[4-( 1,4-dihydroindeno[ 1, 2 -c]pyrazol-3-yl)phenyl]urea: 47 mg of pure product was isolated. LC/MS 381 LC retention time 3.40 minutes.

Example 132: N-[4-(Diethylamino)butyl-N'[4-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenyl] urea: 62 mg of pure product was isolated. LC/MS 418 LC retention time 2.76 minutes.

Example 133: 1,4-Dihydroindeno [1 2 -c]pyrazol-3-yl)phenyl]-N'42-(2-thienyl)ethylurea: mg of pure product was isolated. LC/MS 401 LC retention time 3.44 minutes.

Example 134:

N-[

3 -(Diethylamino)propyl]-N'..[4-(1 ,4-dihydroindeno [1 ,2-c]pyrazol-3yl)phenyl]urea: 50 mg of pure product was isolated. LCIMS 404 LC retention time 2.73 minutes.

Example 135: 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenyl]-N'-[( I-ethyltetrahydro- IH-2pyrrolyl)methyl]urea: 53 mg of pure product was isolated. LC/MS 402 LC retention time 2.89 minutes.

WO 00/27822 WO 0027822PCT/US99/261 122 Example 136: N-(2,5-Difluorobenzyl)-N-[4-( 1,4-dihydroindeno [1 ,2-c]pyrazol-3 -yl)phenyl]urea: mg of pure product was isolated. LC/MS 417 LC retention time 3.50 min Example 137: 1,4-Dihydroindeno [1 ,2-c]pyrazol-3 -yl)phenyl]-N'-[2-(2hydroxyethoxy)ethyl]urea: 34 mng of pure product was isolated. LC/MS 379 LC retention time 2.66 xnin Exam~le 138: 1,4-Dihydroindeno[ 1, 2 -clpyrazol-3-yl)phenyl]-AV-[2-hydroxy- (hydroxymethyl)ethyl]urea: 4 mng of pure product was isolated. LC/M4S 365 LC retention time 2.48 min Example 139: 1,4-Dihydroindeno[1I,2-c]pyrazol-3-yl)phenyl]-N,-(2,3 -dihydroxypropyl)urea: 8 mg of pure product was isolated. LC/MS 365 LC retention time 2.54 min Example 140: Ni 1,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)phenyl]-4-(2-pyridyl)- 1piperazinecarboxamide: 68 mng (7 of pure product was isolated. LCIMS 437 LC retention time 3.13 minutes.

Example 141:

N

1 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N-[3 -(dimethylamino)propyl]- N-methylurea: 54 mng of pure product was isolated. LC/MS 390 LC retention time 2.60 minutes.

WO 00/27822 WO 0027822PCT/US99/26105 123 Example 142: Ni 1,4-Dihydroindeno[ 1,2-clpyrazol-3 -yl)phenyl] -1-azetanecarboxamide: 48 mg of pure product was isolated. LC/MS 331 LC retention time 2.86 minutes.

Examnle 143: Ni -[4-(1I,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-4-(4-fluorophenyl). 1 piperazinecarboxamide: 63 mg of pure product was isolated. LC/MS 454 LC retention time 3.53 minutes.

Example 144: N-Benzyl-N'-[4-( 1,4-dihyclroindeno [1 2 -c]pyrazol-3-yl)phenyl]-N-methylurea: mg of pure product was isolated. LCIMS 395 LC retention time 3.41 minutes.

Example 145: 1,4-Dihydroindeno [1 ,2-c]pyrazol-3-yl)phenyl]-N-ethyl-N-(2hydroxyethyl)urea: 50 mg of pure product was isolated. LC/MS 363 LC retention time 2.78 minutes.

Example 146: Ni 1,4-Dihydroindeno [1 2 -c]pyrazol-3-yl)phenyl]-4-(2-methoxyphenyl)-. 1 piperazinecarboxamide: 49 mg of pure product was isolated. LCIMS 464 (M- LC retention time 3.45 minutes.

Example 147: 1,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)phenyl]-N-[2-(dimethylamino)etliyl]p N-methylurea: 55 mg of pure product was isolated. LC/MS 376 LC retention time 2.48 minutes.

WO 00/27822 WO 0027822PCT/US99/26105 124 Example 148: Ni 1,4-Dihydroindeno [1 ,2-c]pyrazol-3-yl)phenyl]-4-methyl- 1piperazinecarboxamide: 16 mg of pure product was isolated. LC/MS 374 LC retention time 2.48 minutes.

Example, 149: Ni 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-4-(4-hydroxyphenyl)- 1piperazinecarboxamide: 11 mg (11 of pure product was isolated. LC/MS 452 LC retention time 3.04 minutes.

Example 150: NI 1,4-Dihydroindeno[ 1 2 -c]pyrazoI- 3 -yl)phenyl]-4-[(F)-3-phenyl-2-propenyl]p 1-piperazinecarboxamide: 47 mg of pure product was isolated. LC/MS 476 LC retention time 3.26 minutes.

Example 151: NI 1,4-Dihydroindeno[ 1,2-c~pyrazoi-3-yl)phenyl]-4-phenyl- 1piperazinecarboxamide: 46 mg of pure product was isolated. LC/MS 436 LC retention time 3.58 minutes.

Example 152:

N

1 1,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)phenyl]-N,N-di(2-methoxyethyl)urea: 31 mg (3 of pure product was isolated. LC/MS 407 LC retention time 3.25 minutes.

Example 153: 1,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)phenyl]-N-(2,3-dihydroxypropyl>Nmethylurea: 36 mg of pure product was isolated. LC/MS 379 LC retention time 2.62 minutes.

WO 00/27822 WO 0027822PCT/US99/26 105 125 Example 154: N,N-di[2-(Diethylamino)ethyl]-N'-[4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3 yl)phenyl]urea: 50 mg of pure product was isolated. LC/MS 489 LC retention time 2.61 minutes.

Example 155: 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenyl]-N'-(2-pyridylinethyl)urea: 38 mg of pure product was isolated. LC/MS 382 LC retention time 2.85 minutes.

Example 156: 1,4-Dihydroindeno [1,2cprzl3y hnl-M(-yiymty~ra 26 mg (3 1 of pure product was isolated. LC/MS 3 82 LC retention time 2.70 minutes.

Example 157: 1,4-Dihydroindeno[ 1,2cprzl3y hnl-A'(-yiymty~ra 43 mg (5 of pure product was isolated. LC/MS 382 LC retention time 2.69 min Example 158: 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenyl]-N'-(2-hydroxyethyl)urea: 7 mg of pure product was isolated. LC/MS 335 LC retention time 2.57 minutes.

Example 159: 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenyl]-V-[7- (dimethylamino)heptyl]urea: 57 mg of pure product was isolated.L S43 LC retention time 2.85 minutes.

05-01-2001 05-012001US 009926105 2079.1002001 Examples 160 209 Synthesis of the compound 1) RR'NH (=o~did=*n) 2) NaBH, (X GB 2 or 0) assay: ca. 85% (o .i±u rmr lns *condition A: in EtOH, r.t. (o lpai rmr mns condition B: p-TSA (cat.) in toluene, reflux (for secondary or aromatic amines) Entry X NRR' Condition Yield 160 CR2 NHn-C12H25 A (464g 161 CR2 HN~.~OeA 78 mg) 162 CR27 A (430 mg) 163 C.2 A38% 163 C2 HN'~ A(130 mg) 164 CR2 HN- A73 -0 (480 mg) 165 CH282% 165 CR2 A (350mrg) 166 H2 A87% 166 R2 A(370 mg) N H21 167 CH2 Y NB AMENDED SHEET WO 00/27822 WO 0027822PCTIUS99/26105 168 C2 HN B77% 168 CH2 (300 mg) HN H21 169 CH2 NB (21%g N 52% 170 CH2 HN B (210 mg) 171 H2 H N B62% 171 C2 H~~i B(250 mg) 172 0HN A52 172 HN~N.~~MO A(190 mg) 173 0N B73% 173 N Ne B(400 mg) N- 60 174 o HN// B(3m) 0H -N B68% 175 HN-J B(440 mg) 16 0HN--fQ\--NMe 2 B 62% 176 0 (440 mg) 17 N )-NN HIB 33% 177 0 NH (MI) (210 mg) MN 52% 178 0 1 N N B(3m) 179 HN- /N B47% 179 0 \NNjNB (320 mg) 18 sHN,^,N -\0A 61 180 S N~~~N(430 mg) 181 sHN 181 A(130 mg) 182 s HN-(N B 58% mg) 183 s N N B66% 183- S 02~~ (420 mg) 184 S B(240 mg) 185 sH 185 SHN~\,N B(380 mg) 186 CH2 NHiso-Pr A (602%g WO 00/27822 WO 0027822PCTIUS99/261 187 CH2 NHcyc-PR A 188 CH2 Nun-Hex A (32%g 189 CH2 AH A3 (350 mg) 190 CH No 190 H2 NJ B(190 mg) 191 H2 e B76% 191 C2 N ~e B(500 mg) 56% 192 CH2 N\_0 B (360 mg) 22% 193 CH2 HN- OMe B (140 mg) 53% 194 CH2 HN-/ \OH B (360 mg) N 71% 195 CH2 HN /0 B (460 mg) N54 196 CH2 B 350mg 197 CH2 H N A 64% HN (430 mg) HN N 198 H2 \NH B48% 198 CH2 NH (300 mg)

HN

199 CH2 )f B 51% N /(340 mg) WO 00/27822 WO 0027822PCTIUS99/26 105 200 0 No7 B73 (460 mg) 201 HN- N 201 0HN B(430 mg) 202 0 NNMeA 2 ~(350 mg) 203(2HCL) 82% 23 0A (700 mg)

H

N-

N

R Entry X NRR' Condition Yield 204 CH2 HN N/\0(2HCI) A 69% (600 mg) 205 CH2 NoD B 65 (400 mg) 206 H2 B53 C2 (260 mg) 207 CH2 mg) N 73% 208 CH2 HNB (380 mg) 209 CH2 HN- T B (340 mg Exam~1e A The use of compounds of the present invention in the manufacture of pharmaceutical compositions is illustrated by the following description. In this description the term "active compound" denotes any compound of the invention but WO 00/27822 PCT/US99/26105 130 particularly any compound which is the final product of one of the preceding Examples.

a) Capsules In the preparation of capsules, 10 parts by weight of active compound and 240 parts by weight of lactose are de-aggregated and blended. The mixture is filled into hard gelatin capsules, each capsule containing a unit dose or part of a unit dose of active compound.

b) Tablets Tablets are prepared from the following ingredients.

Parts by weight Active compound Lactose 190 Maize starch 22 Polyvinylpyrrolidone Magnesium stearate 3 The active compound, the lactose and some of the starch are de-aggregated, blended and the resulting mixture is granulated with a solution of the polyvinylpyrrolidone in ethanol. The dry granulate is blended with the magnesium stearate and the rest of the starch. The mixture is then compressed in a tabletting machine to give tablets each containing a unit dose or a part of a unit dose of active compound.

c) Enteric coated tablets Tablets are prepared by the method described in above. The tablets are enteric coated in a conventional manner using a solution of 20% cellulose acetate phthalate and 3% diethyl phthalate in ethanol:dichloromethane d) Suppositories In the preparation of suppositories, 100 parts by weight of active compound is incorporated in 1300 parts by weight of triglyceride suppository base and the mixture 131 formed into suppositories each containing a therapeutically effective amount of active ingredient.

Example B Protein Kinase Activity Assays following the procedures described on pages 63-67 were performed on compounds of the present invention to determine protein kinase activity. The results of the assays are set out in the following table. The units of measurement of the results shown in the table are in [M.

*oe• 131a Structure. KRIE KIN KIN' 4. 501 -0.2 -0.04423 0.05981 0.10353 0 N .47229 2.81081 0.05277 N1.4586 0 0 0 oo..

0 0 0 0 131 b StrUctureKD .TE .KIN: KIN 0.0502 0.12496 0.15709, M N0.43605 S0:0813 ,0.26969 594 6.161-95 .75975 19556 N I 0.365 131 c StrKtuR TIE2 StrctreKIN KIN g~ 0.88507 2.09121 2.36612 501 o 0.1686 5 0 I 2:50 01.36359 0 2.18227 501 0.31 872

ICY-

0.57331, i 14542 1.30214,

S

SSS*

**SS

131 d Strctre KDR TIE2 Strctre KIN KIN 0.53868, 0.31 94 0.291 761 13.2998 01.57693 1.51915. 2.62678, 3.53164 0.52232 1.26899 4.41 543 5 0 -~1.52661 C- t .t 4 4

S

131e 131f Str ucture KDR TIE2 KIN. KIN 47 0.4261 1.6784 3.11-28, ,3.8972 10.25491 10.2804 6.661 ~9~1.~0.6821- 1.46171 OA>QF 2.4515 a 131 g K DR., ,,TIE2 StructureKI KN :0.794.6 0.6347 1.1385 0.6812 752 1.7974 2.3627], 3.948

N-N

1281 0 7 "l-81-50 131 h Structure KDA TIE2 1KIN KIN, 3.0437 3.3434 &.6759 a0 1. 1949 3.8441 .2.2882 2.997 Z2755 1.4925 0.306 -4484458' 9 9 131 i Structure' KR -E 2.8862 0.448 0.7107 .91184, .62853, 18022- 0.1T5366 00 1 31j KR TIE2 Structure KIN

KI

9.250331 0o 0.57969 0.09912 .0222 0.6606 36.3898 0.0949 0. 1002. .0.1305 o. 131 k KDR TI52- StructureKN KI 0.1245.

:0.1,977 21 1311 While this invention has been particularly shown and described with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Those skilled in the art will recognise or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described specifically herein. Such equivalents are intended to be encompassed in the scope of the claims.

Comprises/comprising and grammatical variations thereof when used in this specification are to be taken to specify the presence of stated features, integers, steps or components or groups thereof, but do not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof.

e *et **l

Claims

1. A method of inhibiting protein kinase activity comprising the administration of a compound represented by the formula: R 3 R4 X R2 R* NN R 6 RI and pharmaceutically acceptable salts thereof in which X represents a) substituted methylene carbonyl c) oxygen, d) a group of formula -C=NOR7 in which R7 represents H or a C1-4 alkyl group, e) a group of formula NR8 in which R8 represents H, an optionally substituted C1-4 alkyl group or optionally substituted phenyl, f) a group of formula (CH 2 in which n is 1, 2 or 3, or g) a group of formula S(O)p in which p is 0, 1 or 2; R, represents H R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C,6 alkoxy group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C 1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally WO 00/27822 PCT/US99/26105 133 substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group a phenyl C1-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, with the provisos that 1) when X represents carbonyl or a substituted methylene, R1 is hydrogen and R3, R4, R5 and R6 each represent hydrogen, then R2 is not pyridyl, phenyl or phenyl substituted by C1-2 alkyl, a halogen atom, a lower alkoxy group, a hydroxyl group or an amino group; and 2) when X is a group of formula (CH 2 in which n is 1, 2 or 3, R1 is hydrogen and two of R3, R4, R5 and R6 independently represent hydrogen, halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl and the other two represent hydrogen, then R2 is not thienyl, furyl, pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having two WO 00/27822 PCT/US99/26105 134 or less substituents wherein the substituents are halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl; to said kinase in sufficient concentration to inhibit the enzyme activity of said kinase.

2. The method of Claim 1 wherein said protein kinase is a tyrosine kinase.

3. The method of Claim 2 wherein said tyrosine kinase is either a receptor tyrosine kinase or a non-receptor tyrosine kinase.

4. The method of Claim 3 wherein said tyrosine kinase is selected from the group consisting of KDR, fit-1, TIE-2, Lck, Src, fyn and yes. The method of Claim 1 wherein the activity of said tyrosine kinase affects angiogenesis.

6. The method of Claim 5 wherein the inhibition of said tyrosine kinase is anti- angiogenic.

7. The method of Claim 6 wherein said inhibition of said tyrosine kinase inhibits the progression of a disease state selected from the group consisting of cancer, arthritis, atherosclerosis, psoriasis, hemangioma, myocardial angiogenesis, coronary and cerebral collateral vascularization, ischemic limb angiogenesis, corneal disease, rubeosis, neovascular glaucoma, macular degeneration, retinopathy of prematurity, wound healing, ulcers, Helicobacter related diseases, fractures, endometriosis, diabetic retinopathy, cat scratch fever, and thyroid hyperplasia.

8. The method of Claim 2 wherein the activity of said tyrosine kinase affects vascular hyperpermeability or the production of edema.

9. The method of Claim 8, wherein the inhibition of said tyrosine kinase is antiedematous. The method of Claim 9, wherein the inhibition of said tyrosine kinase inhibits the progression of a disease state selected from the group consisting of bums, trauma, chronic lung disease, stroke, polyps, cysts, synovitis, psoriasis, chronic and allergic inflammation, macular degeneration, diabetic retinopathy, retinopathy of prematurity, ovarian hyperstimulation syndrome, pulmonary and cerebral edema, keloid, fibrosis, cirrhosis, carpal tunnel syndrome, adult respiratory distress syndrome, ascites and tumor-associated effusions and edema.

11. The method of Claim 2 wherein the inhibition of said tyrosine kinase has an anti-tumor effect.

12. The method of Claim 2 wherein the inhibition of said tyrosine activity is associated with anti-fertility or abortifacient effects.

13. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents a group of formula in which p represents 0,1 or 2; R, represents H; R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and R3, R4, R5, and R6 are as previously defined; with the provisos that 1) when R1, R3, R4, R5 and R6 each represent hydrogen and X represents SO,, then R' does not represent phenyl and 2) when X represents S and RI, R3, R4, R5 and R6 each represent hydrogen, then R 2 does not represent 2,4-dichlorophenyl. 4 a *444 3 WO 00/27822 PCT/JS99/26105 136

14. Compounds according to Claim 13 in which R 2 represents optionally substituted phenyl, naphthyl, optionally substituted thienyl, optionally substituted pyridyl, optionally substituted furyl, or optionally substituted pyrrolyl. Compounds according Claim 13 in which R. represents 2-thienyl, 3-thienyl, 2-furyl, 3-furyl, 2-pyridyl, 3-pyridyl or 4- pyridyl each of which is optionally substituted, naphthyl and optionally mono-, di- and tri-substituted phenyl, wherein the substituents are selected from optionally substituted alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxymethoxy, 2-carboxyethoxy, 2-carbamoylethoxy, carbamoylmethoxy, 3-carbamoylpropoxy, 2-piperidino-ethoxy, 2-(piperazin-1 -yl)ethoxy, 2- (pyrrolidin-1 -yl)ethoxy, 2-dimethylamino-ethoxy, 2-(perhydro-thiazin-4- yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin-1 -yl)propoxy, 3-(pyrrolidin- 1 yl)-propoxy, 3-dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), lower alkyl (particularly methyl), halo (particularly fluoro and chloro), aryl (particularly phenyl), hydroxy, aryloxy (particularly phenoxy), arylalkoxy (particularly benzyloxy), di-lower-alkylamino (particularly dimethylamino), polyhalo-lower-alkyl, polyhalo-lower-alkoxy (particularly difluoromethoxy), nitro, cyano, lower-alkylthio (particularly methylthio), carboxy, lower- alkoxycarbonyl (particularly methoxycarbonyl), amido (particularly acetamido and benzamido) and optionally substituted carbamoyl (particularly carbamoyl, N-methylcarbanoyl, N-phenylcarbamoyl) and a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro.

16. Compounds according to Claim 13 in which R 2 represents 4-pyridyl, 2-formamidomethyl-4-pyridyl, 2-aminomethyl-4- pynidyl, 2-(hydroxyamidino)-4-pyridyl, 2-carbamoyl-4-pyridyl, 4-pynidyl-N- oxide, 2-chloro-4-pyridyl, 2-cyano-4-pyidyl, 5-methyl-2-furyl, 5-(2-nitro-4- trifluoromethylphenyl)fur-2-yl, phenyl, 4-methoxyphenyl, 3-methoxyphenyl, WO 00/27822PCIS9210 PCTIUS99/26105 137 2-methoxyphenyl, 3 ,4-dimethoxyphenyl, 3,4,5-trimethoxyphenyl, 4-(3- morpholinopropoxy)phenyl, 4-(2-morpholinoethoxy)phenyl, 4-(3-carboxy- propoxy)phenyl, 4-carboxymethoxyphenyl, 4-(3-carbamoylpropoxy)phenyl, 4-carbamoylmethoxyphenyl, 3-(3-morpholino-propoxy)phenyl, 3-(2- morpholino-ethoxy)phenyl, 3-(3-carboxy-propoxy)phenyl, 4- carboxymethoxyphenyl, 3 -(3-carbamoylpropoxy)phenyl, 3- carbamoylrnethoxyphenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 4-hydroxyphenyl, 3-hydroxy-4-rnethoxyphenyl, 4-hydroxy-3- methoxyphenyl, 4-difluoromethoxyphenyl, 3 -nitrophenyl, 4-nitrophenyl, 3,5 di-tert-butyl-4-hydroxyphenyl, 4-methyiphenyl, 4-fluorophenyl, 2- chiorophenyl, 3-chiorophenyl, 4-chiorophenyl, 2,4-dichiorophenyl, 2-chioro- -nitrophenyl, 4-fluoro-2-chlorophenyl, 4-methyithiophenyl, 4-biphenylyl, 3 -phenoxyphenyl, 4-phenoxyphenyl, 4-benzyloxyphenyl, 4- dimethylaminophenyl, 4-diethyl-aminophenyl, 4-methoxycarbonyiphenyl, 4-carbamnoylphenyl, 4-cyanophenyl, 4-N-methylcarbamoylphenyl, 4-N- phenylcarbamoylphenyl, 4-acetamido-phenyl, 4-benzamidophenyl 4- carboxyphenyl, 4-[N-(2-diethylaminoethyl)-carbamoyl]phenyl, 4-(prop- 1- enyloxy)phenyl, 3-(2-hydroxyethoxy)phenyl, 3-(N-(2-diethyl aminoethyl)- carbamoylmethoxy)phenyl, 3- [3-(N-(2-diethylamino-ethyl)carbamoyl) propoxy]phenyl, 4-(N-(2-diethylaminoethyl)carbamoyl-methoxy)phenyl, 4- [3-(N-(2-diethylaminoethyl)-carbamoyl)propoxy]phenyl, 2-furyl, 5-[3,5- bis(trifluoromethyl)phenyl]-2-fuiryl, 3-bromo-2-thienyl, 5-methoxy-2-fuiryl, -(2-nitro-4-trifluoromethylphenyl)-2-furyl, 3-N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl, 3 -[3-(N-(2-morpholinoethyl) carbamoyl)propoxy-phenyl], 4-(N-(2-morpholino ethyl)- carbamoylmethoxy)phenyl 4-(morpholinacetamido)phenyl and 4-[3 morpholinoethyl)carbamoyl)propoxy~phenyl.

17. Compounds according to Claim 13 in which R 3 R, and R 6 independently represent hydrogen halo (particularly fluoro), optionally substituted lower alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxy- 138 methoxy, 2-carboxyethoxy, 2-carbamoylethoxy, 3 -carbamoylpropoxy, 2- piperidinoethoxy, 2-(piperazin-l -yl)ethoxy, 2-(pyrrolidin-1I-yl)ethoxy, 2- dimethylaminoethoxy, 2-(perhydrothiazin- 1-yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin- 1-yl)propoxy, 3-(pyrrolidin-l -yl)propoxy, 3- dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), carbamoylmethoxy, hydroxypropyloxy, hydroxyethoxy, (3- morpholino)propoxy and (2-morpholino)ethoxy), amido (particularly acetamido and benzamido), optionally substituted carbamoyl (particularly carbamnoyl, N-methyl-carbamoyl and N-phenylcarbamoyl), carboxy, nitro and amino.

18. Compounds according to Claim 13 in which R 3 R, and R, represent the following 6,7-dimethoxy, 6,7,8-trimethoxy, 6- fluoro, 6-acetarmido, 7-methoxy, 6-carbamoyl, 6-(N-methylcarbamoyl), 6-(N- phenylcarbamoyl), 3-morpholinopropoxy and 2-morpholinoethoxy.

19. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents oxygen; 20 R, represents H; 6666 R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and R3, R4, R5 and R6 are as previously defined; with the proviso that when Ri, R3, R4, R5 and R6 each represent hydrogen, then R 2 does not represent phenyl, 2,4-dimethylphenyl or 2,4-dichlorophenyl. Compounds according to Claim 19 in which R, represents optionally 6666 substituted phenyl, naphthyl, Qptionally substituted thienyl, optionally substituted pyridyl, optionally substituted furyl, or optionally substituted 6666 pyrrolyl. WO 00/27822 WO 0027822PCT/US99/26105 139

21. Compounds according Claim 19 in which R 2 represents 2-thienyl, 3-thienyl, 2-furyl, 3-furyl, 2-pyridyl, 3-pyridyl or 4- pyridyl each of which is optionally substituted, naphthyl and optionally mono-, di- and tni-substituted phenyl, wherein the substituents are selected from optionally substituted alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3 -carboxypropoxy, carboxymethoxy, 2-carboxyethoxy, 2-carbamnoylethoxy, carbamnoylmethoxy, 3-carbamoylpropoxy, 2-piperidino-ethoxy, 2-(piperazin- 1-yl)ethoxy, 2- (pyrrolidin- 1-yl)ethoxy, 2-dimethylamino-ethoxy, 2-(perhydro-thiazin-4- yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin-l-yl)propoxy, 3-(pyrrolidin- 1 yl)-propoxy, 3-dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), lower alkyl (particularly methyl), halo (particularly fluoro and chloro), aryl (particularly phenyl), hydroxy, aryloxy (particularly phenoxy), arylalkoxy (particularly benzyloxy), di-lower-alkylamino (particularly dimethylamino), polyhalo-lower-alkyl, polyhalo-lower-alkoxy (particularly difluoromethoxy), nitro, cyano, lower-alkylthio, (particularly methylthio), carboxy, lower- alkoxycarbonyl (particularly niethoxycarbonyl), amido (particularly acetamido and benzamido) and optionally substituted carbamnoyl (particularly carbamnoyl, N-methylcarbamoyl, N-phenylcarbamoyl) and a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro.

22. Compounds according to Claim 19 in which R, represents 4-pyridyl, 2-formamidomethyl-4-pyridyl, 2-aminomethyl-4- pyridyl, 2-(hydroxyamidino)-4-pyridyl, 2-carbamoyl-4-pyridyl, 4-pyridyl-N- oxide, 2-chloro-4-pyridyl, 2-cyano-4-pyridyl, 5-methyl-2-furyl, 5-(2-nitro-4- trifluoromethylphenyl)fur-2-yl, phenyl, 4-methoxyphenyl, 3-methoxyphenyl, 2-methoxyphenyl, 3 ,4-dimethoxyphenyl, 3 ,4,5-trimethoxyphenyl, 4-(3- morpholinopropoxy)phenyl, 4-(2-morpholinoethoxy)phenyl, 4-(3-carboxy- propoxy)phenyl, 4-carboxymethoxyphenyl, 4-(3-carbamnoylpropoxy)phenyl, 4-carbamoylmethoxyphenyl, 3-(3-morpholino-propoxy)phenyl, 3-(2- morpholino-ethoxy)phenyl, 3-(3-carboxy-propoxy)phenyl, 4- WO 00/27822 WO 0027822PCTIUS99/26105 140 carboxyrnethoxyphenyl, 3 -(3-carbamoylpropoxy)phenyl, 3- carbamoylmethoxyphenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 4-hydroxyphenyl, 3 -hydroxy-4-methoxyphenyl, 4-hydroxy-3- methoxyphenyl, 4-difluoromethoxyphenyl, 3-nitrophenyl, 4-nitrophenyl, di-tert-butyl-4-hydroxyphenyl, 4-methyiphenyl, 4-fluorophenyl, 2- chiorophenyl, 3-chiorophenyl, 4-chiorophenyl, 2,4-dichiorophenyl, 2-chioro- -nitrophenyl, 4-fluoro-2-chlorophenyl, 4-methyithiophenyl, 4-biphenylyl, 3 -phenoxyphenyl, 4-phenoxyphenyl, 4-benzyloxyphenyl, 4- dimethylaminophenyl, 4-diethyl-aminophenyl, 4-methoxycarbonyiphenyl, 4-carbamoylphenyl, 4-cyanophenyl, 4-N-methylcarbamoylphenyl, 4-N- phenylcarbamoylphenyl, 4-acetamido-phenyl, 4-beazamidophenyl 4- carboxyphenyl, 4-[N-(2-diethylaminoethyl)-carbamoyl]phenyl, 4-(prop-1 enyloxy)phenyl, 3-(2-hydroxyethoxy)phenyl, 3-(N-(2-diethyl aminoethyl)- carbamoylmethoxy)phenyl, 3-[3-(N-(2-diethylamnino-ethyl)carbamnoyl) propoxylphenyl, 4-(N-(2-diethylaminoethyl)carbanioyl-methoxy)phenyl, 4- [3-(N-(2-diethylaminoethyl)-carbamoyl)propoxy]phenyl, 2-fuiryl, 5-[3 bis(trifluoromethyl)phenyl]-2-furyl, 3-bromo-2-thienyl, 5-methoxy-2-furyl, 5-(2-nitro-4-trifluoromethylphenyl)-2-furyl, 3-N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl, 3-[3-(N-(2-morpholinoethyl) carbamoyl)propoxy-phenyl], 4-(N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl, 4-(morpholinacetamido)phenyl and morpholinoethyl)carbamoyl)propoxy]phenyl.

23. Compounds according to Claim 19 in which R 3 R 4 R, and R, independently represent haydrogen halo (particularly fluoro), optionally substituted lower alkoxy (particularly methoxy, 3-morpholinopropoxy, 2-morpholinoethoxy, 3- carboxypropoxy, carboxy-methoxy, 2-carboxyethoxy, 2-carbamoylethoxy, 3- carbaxnoylpropoxy, 2-piperidinoethoxy, 2-(piperazin- 1-yl)ethoxy, 2- (pyrrolidin- 1-yl)ethoxy, 2-dimethylaminoethoxy, 2-(perhydrothiazin- 1- yl)ethoxy, 3-pip eridinopropoxy, 3-(piperazin- 1-yl)propoxy, 3-(pyrrolidin- 1- yl)propoxy, 3-dimethylamninopropoxy, 3-(perhydrothiazin-4-yl)propoxy), carbamoylmethoxy, hydroxypropyloxy, hydroxyethoxy, (3- morpholino)propoxy and (2-morpholino)ethoxy), amido (particularly acetamido and benzamido), optionally substituted carbamoyl (particularly carbamoyl, N-methyl-carbamoyl and N-phenylcarbamoyl), carboxy, nitro and amino.

24. Compounds according to Claim 19 in which R 3 R 4 R, and R 6 represent the following 6,7-dimethoxy, 6,7,8-trimethoxy, 6- fluoro, 6-acetamido, 7-methoxy, 6-carbamoyl, 6-(N-methylcarbamoyl), 6-(N- phenylcarbamoyl), 3-morpholinopropoxy and 2-morpholinoethoxy. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents a group of formula NR,; R, represents H; R, represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and R3, R4, R5 and R6 are as previously defined.

26. Compounds according to Claim 25 in which R, represents optionally 20 substituted phenyl, naphthyl, optionally substituted thienyl, optionally substituted pyridyl, optionally substituted furyl, or optionally substituted pyrrolyl.

27. Compounds according Claim 25 in which R, represents 2-thienyl, 3-thienyl, 2-furyl, 3-furyl, 2-pyridyl, 3-pyridyl or 4- pyridyl each of which is optionally substituted, naphthyl and optionally mono-, di- and tri-substituted phenyl, wherein the substituents are selected from optionally substituted alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, 30 carboxymethoxy, 2-carboxyethoxy, 2-carbamoylethoxy, carbamoylmethoxy, 3-carbamoylpropoxy, 2-piperidino-ethoxy, 2-(piperazin-1-yl)ethoxy, 2- (pyrrolidin-1-yl)ethoxy, 2-dimethylamino-ethoxy, 2-(perhydro-thiazin-4- WO 00/27822 WO 0027822PCT[US99/261 142 yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin-l -yl)propoxy, 3-(pyrrolidin- I yl)-propoxy, 3-dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), lower alkyl (particularly methyl), halo (particularly fluoro and chioro), aryl (particularly phenyl), hydroxy, aryloxy (particularly phenoxy), arylalkoxy (particularly benzyloxy), di-lower-alkylamino (particularly dimethylamino), polyhalo-lower-alkyl, polyhalo-lower-alkoxy (particularly difluoromethoxy), nitro, cyano, lower-alkylthio (particularly methylthio), carboxy, lower- alkoxycarbonyl (particularly methoxycarbonyl), amido (particularly acetamido and benzamido) and optionally substituted carbamnoyl (particularly carbamoyl, N-methylcarbamoyl, N-phenylcarbamoyl) and a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro.

28. Compounds according to Claim 25 in which R 2 represents 4-pyridyl, 2-formamidomethyl-4-pyridyl, 2-aminomethyl-4- pyridyl, 2-(hydroxyamidino)-4-pyridyl, 2-carbamoyl-4-pyridyl, 4-pyridyl-N- oxide, 2-chloro-4-pyridyl, 2-cyano-4-pyridyl, 5-methyl-2-furyl, 5-(2-nitro-4- trifluoromethylphenyl)fur-2-yl, phenyl, 4-methoxyphenyl, 3-methoxyphenyl, 2-methoxyphenyl, 3 ,4-dimethoxyphenyl, 3 ,4,5-trimethoxyphenyl, 4-(3- morpholinopropoxy)phenyl, 4-(2-morpholinoethoxy)phenyl, 4-(3-carboxy- propoxy)phenyl, 4-carboxymethoxyphenyl, 4-(3-carbamnoylpropoxy)phenyl, 4-carbamoylmethoxyphenyl, 3-(3-morpholino-propoxy)phenyl, 3-(2- morpholino-ethoxy)phenyl, 3-(3-carboxy-propoxy)phenyl, 4- carboxymethoxyphenyl, 3-(3-carbamoylpropoxy)phenyl, 3- carbamoylmethoxyphenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 4-hydroxyphenyl, 3-hydroxy-4-methoxyphenyl, 4-hydroxy-3- methoxyphenyl, 4-difluoromethoxyphenyl, 3-nitrophenyl, 4-nitrophenyl, di-tert-butyl-4-hydroxyphenyl, 4-methyiphenyl, 4-fluorophenyl, -2- chlorophenyl, 3-chlorophenyl, 4-chlorophenyl, 2,4-dichiorophenyl, 2-chloro- 5 -nitrophenyl, 4-fluoro-2-chlorophenyl, 4-methylthiophenyl, 4-biphenylyl, 3-phenoxyphenyl, 4-phenoxyphenyl, 4-benzyloxyphenyl, 4- dimethylaminophenyl, 4-diethyl-aminophenyl, 4-methoxycarbonylphenyl, WO 00/27822 WO 0027822PCTIUS99/26 105 143 4-carbamoylphenyl, 4-cyanophenyl, 4-N:-methylcarbamoylphenyl, 4-N- phenylcarbamoylphenyl, 4-acetamido-phenyl, 4-benzamidophenyl 4- carboxyphenyl, 4-[N-(2-diethylaminoethyl)-carbamoyl]phenyl, 4-(prop- I1- enyloxy)phenyl, 3-(2-hydroxyethoxy)phenyl, 3-(N-(2-diethyl aminoethyl)- carbamoylmethoxy)phenyl, 3-[3-(N-(2-diethylamino-ethyl)carbamoyl) propoxy]phenyl, 4-(N-(2-diethylaminoethyl)carbaxnoyl-methoxy)phenyl, 4- 3 -(N-(2-diethylaminoethyl)-carbamoyl)propoxy]phenyl, 2-furyl, 5-[3 ,5 bis(trifluoromethyl)phenyl] -2-fuiryl, 3-bromo-2-thienyl, 5-methoxy-2-fiiryl, -(2-nitro-4-trifluoromethylphenyl)-2-fiiryl, 3-N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl, 3- [3 -(N-(2-morpholino ethyl) carbamoyl)propoxy-phenyl], 4-(N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl 4-(morpholinacetamido)phenyl and morpholinoethyl)carbamoyl)propoxy]phenyl.

29. Compounds according to Claim 25 in which R 3 R, and R, independently represent hydrogen, halo (particularly fluoro), optionally substituted lower alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxy- methoxy, 2-carboxyethoxy, 2-carbamoylethoxy, 3 -carbamoylpropoxy, 2- piperidinoethoxy, 2-(piperazin- 1-yl)ethoxy, 2-(pyrrolidin- 1-yl)ethoxy, 2- dimethylaminoethoxy, 2-(perhydrothiazin- 1 -yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin- 1-yl)propoxy, 3-(pyrrolidin-1I-yl)propoxy, 3- dimnethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), carbamoylmethoxy, hydroxypropyloxy, hydroxyethoxy, (3- morpholino)propoxy and (2-morpholino)ethoxy), amido (particularly acetamido and benzamido), optionally substituted carbamoyl (particularly carbamoyl, N-methyl-carbamoyl and N-phenylcarbaxnoyl), carboxy, nitro and amino. 144 Compounds according to Claim 25 in which R 3 R 4 R, and R, represent the following 6,7-dimethoxy, 6,7,8-trimethoxy, 6- fluoro, 6-acetamido, 7-methoxy, 6-carbamoyl, 6-(N-methylcarbamoyl), 6-(N- phenylcarbamoyl), 3-morpholinopropoxy and 2-morpholinoethoxy.

31. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents a group of formula -C=NOR7 in which R7 represents H or a C 1-4 alkyl group; R, represents H; R, represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and R 3 R 4 and R, are as previously defined; with the proviso that when RI, R3, R4, R5 and R6 each represent hydrogen, and X represents C=NOH then R2 does not represent 4-methylphenyl or 3,4-dimethoxyphenyl.

32. Compounds according to Claim 31 in which R, represents optionally substituted phenyl, naphthyl, optionally substituted thienyl, optionally 20 substituted pyridyl, optionally substituted furyl, or optionally substituted pyrrolyl.

33. Compounds according Claim 31 in which R, represents 2-thienyl, 3-thienyl, 2-furyl, 3-furyl, 2-pyridyl, 3-pyridyl or 4- pyridyl each of which is optionally substituted, naphthyl and optionally monol, di- and tri-substituted phenyl, wherein the substituents are selected from optionally substituted alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxymethoxy, 2-carboxyethoxy, 2-carbamoylethoxy, carbamoylmethoxy, 3-carbamoylpropoxy, 2-piperidino-ethoxy, 2-(piperazin-I -yl)ethoxy, 2- (pyrrolidin-1-yl)ethoxy, 2-dimethylamino-ethoxy, 2-(perhydro-thiazin-4- yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin-1-yl)propoxy, 3-(pyrrolidin- WO 00/27822 WO 0027822PCT/US99/26 105 145 1 yl)-propoxy, 3-dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), lower alkyl (particularly methyl), halo (particularly fluoro and chloro), aryl (particularly phenyl), hydroxy, aryloxy (particularly phenoxy), arylalkoxy (particularly benzyloxy), di-lower-alkylamino (particularly dimethylamino), polyhalo-lower-alkyl, polyhalo-lower-alkoxy (particularly difluoromethoxy), nitro, cyano, lower-alkylthio (particularly methylthio), carboxy, lower- alkoxycarbonyl (particularly methoxycarbonyl), amido (particularly acetamido, and benzamido) and optionally substituted carbamoyl (particularly carbamoyl, N-methylcarbamnoyl, N-phenylcarbamoyl) and a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro.

34. Compounds according to Claim 31 in which R 2 represents 4-pyridyl, 2-formamidomethyl-4-pyridyl, 2-aminomethyl-4- pyridyl, 2-(hydroxyamidino)-4-pyridyl, 2-carbamoyl-4-pyridyl, 4-pyridyl-N- oxide, 2-chloro-4-pyridyl, 2-cyano-4-pyridyl, 5-methyl-2-furyl, 5-(2-nitro-4- trifluoromethylphenyl)fur-2-yl, phenyl, 4-methoxyphenyl, 3-methoxyphenyl, 2-methoxyphenyl, 3 ,4-dimethoxyphenyl, 3 ,4,5-trimethoxyphenyl, 4-(3 morpholinopropoxy)phenyl, 4-(2-morpholinoethoxy)phenyl, 4-(3-carboxy- propoxy)phenyl, 4-carboxymethoxyphenyl, 4-(3-carbamoylpropoxy)phenyl, 4-carbamoylmethoxyphenyl, 3-(3-morpholino-propoxy)phenyl, 3-(2- morpholino-ethoxy)phenyl, 3-(3-carboxy-propoxy)phenyl, 4- carboxymethoxyphenyl, 3-(3 -carbamoylpropoxy)phenyl, 3- carbamnoylmethoxyphenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 4-hydroxyphenyl, 3-hyclroxy-4-methoxyphenyl, 4-hydroxy-3- methoxyphenyl, 4-difluoromethoxyphenyl, 3-nitrophenyl, 4-nitrophenyl, di-tert-butyl-4-hydroxyphenyl, 4-methylphenyl, 4-fluorophenyl, 2- chlorophenyl, 3-chiorophenyl, 4-chlorophenyl, 2,4-dichlorophenyl, 2-chioro- 4-fluoro-2-chlorophenyl, 4-methylthiophenyl, 4-biphenylyl, 3-phenoxyphenyl, 4-phenoxyphenyl, 4-benzyloxyphenyl, 4- dimethylarninophenyl, 4-diethyl-aminophenyl, 4-methoxycarbonyiphenyl, 4-carbamoylphenyl, 4-cyanophenyl, 4-N-methylcarbamoylphenyl, 4-N- WO 00/27822 WO 0027822PCT/US99/261 146 phenylcarbamoylphenyl, 4-acetamido-phenyl, 4-berizamidophenyl 4- carboxyphenyl, 4-1N-(2-diethylaminoethyl)-carbamoyl]phenyl, 4-(prop- 1- enyloxy)phenyl, 3-(2-hydroxyethoxy)phenyl, 3-(N-(2-diethyl aminoethyl)- carbamoylmethoxy)phenyl, 3 -[3-(N-(2-diethylamnino-ethyl)carbamoyl) propoxylphenyl, 4-(N-(2-diethylaminoethyl)carbamoyl-methoxy)phenyl, 4- [3-(N-(2-diethylaminoethyl)-carbamoyl)propoxy]phenyl, 2-furyl, 5-[3 bis(trifluoromethyl)phenyl]-2-furyl, 3-bromo-2-thienyl, 5-methoxy-2-furyl, 5-(2-nitro-4-trifluoromethylphenyl)-2-furyl, 3 -morpholinoethyl)- carbamoylmethoxy)phenyl, 3- [3 -(N-(2-morpholino ethyl) carbamoyl)propoxy-phenyl], 4-(N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl 4-(morpholinacetamido)phenyl and morpholinoethyl)carbamoyl)propoxy]phenyl. Compounds according to Claim 31 in which R 3 R 4 R, and R independently represent hydrogen, halo (particularly fluoro), optionally substituted lower alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3 -carboxypropoxy, carboxy- methoxy, 2-carboxyethoxy, 2-carbamoylethoxy, 3-carbamoylpropoxy, 2- piperidinoethoxy, 2-(piperazin- 1 -yl)ethoxy, 2-(pyrrolidin- 1 -yl)ethoxy, 2- dimethylarninoethoxy, 2-(perhydrothiazin- 1-yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin- 1-yl)propoxy, 3-(pyrrolidin- 1-yl)propoxy, 3- dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), carbamoylmethoxy, hydroxypropyloxy, hydroxyethoxy, (3- morpholino)propoxy and (2-morpholino)ethoxy), amido (particularly acetamido and benzamnido), optionally substituted carbamoyl (particularly carbamoyl, N-methyl-carbamnoyl and N-phenylcarbamoyl), carboxy, nitro and amino.

36. Compounds according to Claim 31 in which R 3 R 5 and R 6 represent the following 6,7-dimethoxy, 6,7,8-trimethoxy, 6- fluoro, 6-acetamido, 7-methoxy, 6-carbamnoyl, 6-(N-methylcarbamoyl), 6-(N- phenylcarbamoyl), 3-morpholinopropoxy and 2-morpholinoethoxy.

37. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents substituted methylene or a carbonyl group; R, represents H; R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; and R3, R4, R5, and R6 are as previously defined; with the provisos that 1) when X represents carbonyl or a substituted methylene, R1 is hydrogen and R3, R4, R5 and R6 each represent hydrogen, then R2 is not pyridyl, 2-thienyl, 3-thienyl, phenyl or phenyl substituted by C 1-2 alkyl, a halogen atom, a lower alkoxy group, a hydroxyl group or an amino group; 2) when X represents methylene, R1 is hydrogen and two of R3, R4, and R6 independently represent hydrogen, halogen having an atomic weight of about 19 to 36, Cl-4 alkyl, C1-4 alkoxy or trifluoromethyl and the other two represent hydrogen, then R2 is not thienyl, furyl, S* .pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having 20 two or less substituents wherein the substituents are halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl; and 3) when X represents a carbonyl group, R2 represents phenyl, 4- chlorophenyl or 4-methoxyphenyl, then R3, R4, R5 and R6 are not trifluoromethyl; and 4) when X represents a carbonyl group, R2 represents phenyl, R3 represents bromo, R4 represents hydroxy and R5 represents methoxy, S 30 then R6 is not hydrogen and *~l WO 00/27822 PCT/US99/26105 148 when X represents carbonyl and R3, R4, R5 and R6 represent hydrogen, then R6 is not aryl.

38. Compounds according to Claim 37 in which R. represents optionally substituted phenyl, naphthyl, optionally substituted thienyl, optionally substituted pyridyl, optionally substituted furyl, or optionally substituted pyrrolyl.

39. Compounds according Claim 37 in which R, represents 2-thienyl, 3-thienyl, 2-furyl, 3-furyl, 2-pyridyl, 3-pyridyl or 4- pyridyl each of which is optionally substituted, naphthyl and optionally mono-, di- and tri-substituted phenyl, wherein the substituents are selected from optionally substituted alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxymethoxy, 2-carboxyethoxy, 2-carbamoylethoxy, carbamoylmethoxy, 3-carbamoylpropoxy, 2-piperidino-ethoxy, 2-(piperazin-1-yl)ethoxy, 2- (pyrrolidin- 1 -yl)ethoxy, 2-dimethylamino-ethoxy, 2-(perhydro-thiazin-4- yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin-1-yl)propoxy, 3-(pyrrolidin- lyl)-propoxy, 3-dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), lower alkyl (particularly methyl), halo (particularly fluoro and chloro), aryl (particularly phenyl), hydroxy, aryloxy (particularly phenoxy), arylalkoxy (particularly benzyloxy), di-lower-alkylamino (particularly dimethylamino), polyhalo-lower-alkyl, polyhalo-lower-alkoxy (particularly difluoromethoxy), nitro, cyano, lower-alkylthio (particularly methylthio), carboxy, lower- alkoxycarbonyl (particularly methoxycarbonyl), amido (particularly acetamido and benzamido) and optionally substituted carbamoyl (particularly carbamoyl, N-methylcarbamoyl, N-phenylcarbamoyl) and a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro. WO 00/27822 WO 0027822PCT/US99/261 149 Compounds according to Claim 37 in which R 2 represents 4-pyridyl, 2-formamidomethvl-4-pyridyl, 2-aminomethyl-4- pyridyl, 2-(hydroxyamidino)-4-py-idyl, 2-carbamoyl-4-pyridyl, 4-pyridyl-N- oxide, 2-chloro-4-pyridyl, 2-cyano-4-pyridyl, 5-methyl-2-fuiryl, 5-(2-nitro-4- trifluoromethylphenyl)far-2-yl, phenyl, 4-methoxyphenyl, 3-methoxyphenyl, 2-methoxyphenyl, 3 ,4-dimethoxyphenyl, 3 ,4,5-trimethoxyphenyl, 4-(3- morpholinopropoxy)phenyl, 4-(2-morpholinoethoxy)phenyl, 4-(3-carboxy- propoxy)phenyl, 4-carboxymethoxyphenyl, 4-(3 -carbamoylpropoxy)phenyl, 4-carbamoylmethoxyphenyl, 3-(3-morpholino-propoxy)phenyl, 3-(2- morpholino-ethoxy)phenyl, 3-(3-carboxy-propoxy)phenyl, 4- carboxyrnethoxyphenyl, 3-(3-carbamoylpropoxy)phenyl, 3- carbamoylmethoxyphenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 4-hydroxyphenyl, 3-hydroxy-4-methoxyphenyl, 4-hydroxy-3- methoxyphenyl, 4-difluoromethoxyphenyl, 3-nitrophenyl, 4-nitrophenyl, di-tert-butyl-4-hydroxyphenyl, 4-methyiphenyl, 4-fluorophenyl, 2- chiorophenyl, 3-chiorophenyl, 4-chiorophenyl, 2,4-dichiorophenyl, 2-chioro- 4-fluoro-2-chlorophenyl, 4-methyithiophenyl, 4-biphenylyl, 3 -phenoxyphenyl, 4-phenoxyphenyl, 4-benzyloxyphenyl, 4- dimethylaminophenyl, 4-diethyl-aminophenyl, 4-methoxycarbonyiphenyl, 4-carbamoylphenyl, 4-cyanophenyl, 4-N-methylcarbamoylphenyl, 4-N- phenylcarbamoylphenyl, 4-acetamido-phenyl, 4-benzamnidophenyl 4- carboxyphenyl, 4-[N-(2-diethylaminoethyl)-carbamoyl]phenyl, 4-(prop- 1- enyloxy)phenyl, 3-(2-hydroxyethoxy)phenyl, 3-(N-(2-diethyl aminoethyl)- carbamoylmethoxy)phenyl, 3-[3-(N-(2-diethylamino-ethyl)carbamoyl) propoxyjphenyl, 4-(N-(2-diethylaminoethyl)carbamoyl-methoxy)phenyl, 4- [3-(N-(2-diethylaminoethyl)-carbamnoyl)propoxy~phenyl, 2-furyl, 5- bis(trifluoromethyl)phenyl]-2-furyl, 3-bromo-2-thienyl, 5-methoxy-2-furyl, 5-(2-nitro-4-trifluoromethylphenyl)-2-furyl, 3-N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl, 3-[3-(N-(2-morpholinoethyl) carbamnoyl)propoxy-phenyl], 4-(N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl 4-(morpholinacetamido)phenyl and morpholinoethyl)carbamoyl)propoxy]phenyl.

41. Compounds according to Claim 37 in which R, and R, independently represent hydrogen halo (particularly fluoro), optionally substituted lower alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxy- methoxy, 2-carboxyethoxy, 2-carbamoylethoxy, 3-carbamnoylpropoxy, 2- piperidinoethoxy, 2-(piperazin- 1-yl)ethoxy, 2-(pyrrolidin- 1-yl)ethoxy, 2- dimethylaminoethoxy, 2-(perhydrothiazin-1I-yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin- 1-yl)propoxy, 3-(pyrrolidin- 1-yl)propoxy, 3- dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), carbanioylmethoxy, hydroxypropyloxy, hydroxyethoxy, (3- morpholino)propoxy and (2-morpholino)ethoxy), amido (particularly acetamido and benzamido), optionally substituted carbamnoyl (particularly carbanioyl, N-methyl-carbamoyl and N-phenylcarbamoyl), carboxy, nitro and amino.

42. Compounds according to Claim 37 in which R 3 R 4 R, and R, represent the following 6,7-dimethoxy, 6,7,8-trimethoxy, 6- fluoro, 6-acetamido, 7-methoxy, 6-carbamoyl, 6-(N-methylcarbamoyl), 6-(N- phenylcarbarnoyl), 3-morpholinopropoxy and 2-morpholinoethoxy.

43. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: *too*:X represents a group of formula (CH2)n in which n is 1, 2 or 3 R, represents H; R 2 represents aryl, pyridyl, thienyl, fliryl or pyrrolyl each of which is optionally substituted; and R 3 P, and R, are as previously defined with the proviso that when X is a group of formula (ICH 2 in which n is 1, 2 or 3, RI is hydrogen 30 and two of R3, R4, R5 and R6 independently represent hydrogen, halogen having an atomic weight of about 19 to 36, CI-4 alkyl, C1-4 ailkoxy or trifluoromethyl and the other two represent hydrogen, then R2 is not thienyl, WO 00/27822 PCT/US99/26105 151 furyl, pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having two or less substituents wherein the substituents are halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl, and with the proviso that when n is 2 and R3, R4, R5 and R6 each represent hydrogen or methoxy, then R2 does not represent 3-carboxypyrid-2-yl, 3-methoxycarbonylpyrid-2- yl or 2-carboxyphenyl

44. Compounds according to Claim 43 in which R 2 represents optionally substituted phenyl, naphthyl, optionally substituted thienyl, optionally substituted pyridyl, optionally substituted furyl, or optionally substituted pyrrolyl. Compounds according Claim 43 in which R, represents 2-thienyl, 3-thienyl, 2-furyl, 3-furyl, 2-pyridyl, 3-pyridyl or 4- pyridyl each of which is optionally substituted, naphthyl and optionally mono-, di- and tri-substituted phenyl, wherein the substituents are selected from optionally substituted alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxymethoxy, 2-carboxyethoxy, 2-carbamoylethoxy, carbamoylmethoxy, 3-carbamoylpropoxy, 2-piperidino-ethoxy, 2-(piperazin-1 -yl)ethoxy, 2- (pyrrolidin-1-yl)ethoxy, 2-dimethylamino-ethoxy, 2-(perhydro-thiazin-4- yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin-1-yl)propoxy, 3-(pyrrolidin- lyl)-propoxy, 3-dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), lower alkyl (particularly methyl), halo (particularly fluoro and chloro), aryl (particularly phenyl), hydroxy, aryloxy (particularly phenoxy), arylalkoxy (particularly benzyloxy), di-lower-alkylamino (particularly dimethylamino), polyhalo-lower-alkyl, polyhalo-lower-alkoxy (particularly difluoromethoxy), nitro, cyano, lower-alkylthio (particularly methylthio), carboxy, lower- alkoxycarbonyl (particularly methoxycarbonyl), amido (particularly acetamido and benzamido) and optionally substituted carbamoyl (particularly carbamoyl, N-methylcarbamoyl, N-phenylcarbamoyl) and a pyridyloxy or WO 00/27822 WO 0027822PCT[US99/26105 152 pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro.

46. Compounds according to Claim 43 in which R, represents 4-pyridyl, 2-formamidomethyl-4-pyridyl, 2-aminomethyl-4- pyridyl, 2-(hydroxyamidino)-4-pyridyl, 2-carbamoyl-4-pyridyl, 4-pyridyl-N- oxide, 2-chloro-4-pyridyl, 2-cyano-4-pyridyl, 5-methyl-2-furyl, 5-(2-nitro-4- trifluoromethylphenyl)fur-2-yl, phenyl, 4-methoxyphenyl, 3-methoxyphenyl, 2-methoxyphenyl, 3 ,4-dimethoxyphenyl, 3 ,4,5-trimethoxyphenyl, 4-(3- morpholinopropoxy)phenyl, 4-(2-morpholinoethoxy)phenyl, 4-(3-carboxy- propoxy)phenyl, 4-carboxymethoxyphenyl, 4-(3 -carbamoylpropoxy)phenyl, 4-carbamoylmnethoxyphenyl, 3-(3-morpholino-propoxy)phenyl, 3 morpholino-ethoxy)phenyl, 3-(3-carboxy-propoxy)phenyl, 4- carboxymethoxyphenyl, 3-(3-carbamoylpropoxy)phenyl, 3- carbamoylmethoxyphenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 4-hydroxyphenyl, 3-hydroxy-4-methoxyphenyl, 4-hydroxy-3- methoxyphenyl, 4-difluoromethoxyphenyl, 3 -nitrophenyl, 4-nitrophenyl, di-tert-butyl-4-hydroxyphenyl, 4-methyiphenyl, 4-fluorophenyl, 2- chiorophenyl, 3-chiorophenyl, 4-chlorophenyl, 2 ,4-dichlorophenyl, 2-chioro- 5-nitrophenyl, 4-fluoro-2-chlorophenyl, 4-methyithiophenyl, 4-biphenylyl, 3-phenoxyphenyl, 4-phenoxyphenyl, 4-benzyloxyphenyl, 4- dimethylaminophenyl, 4-diethyl-aminophenyl, 4-methoxycarbonylphenyl, 4-carbamoylphenyl, 4-cyanophenyl, 4-N-methylcarbamoylphenyl, 4-N- phenylcarbamoylphenyl, 4-acetamido-phenyl, 4-benzamnidophenyl 4- carboxyphenyl, 4- [N-(2-diethylaminoethyl)-carbamoyljphenyl, 4-(prop- 1- enyloxy)phenyl, 3-(2-hydroxyethoxy)phenyl, 3-(N-(2-diethyl aminoethy])- carbamoylmethoxy)phenyl, 3-[3-(N-(2-diethylamino-ethyl)carbamoyl) propoxyiphenyl, 4-(N-(2-diethylaminoethyl)carbamnoyl-methoxy)phenyl, 4- [3-(N-(2-diethylaminoethyl)-carbamoyl)propoxy]phenyl, 2-furyl, 5-[3 bis(trifluoromethyl)phenyl]-2-furyl, 3-bromo-2-thienyl, 5-methoxy-2-furyl, 5-(2-nitro-4-trifluoromethylphenyl)-2-fiyl, 3-N-(2-morpholinoethyl)- carbamnoylmethoxy)phenyl, 3 -[3-(N-(2-morpholinoethyl) carbamnoyl)propoxy-phenyl], 4-(N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl 4-(morpholinacetamido)phenyl and morpholinoethyl)carbamoyl)propoxy]phenyl.

47. Compounds according to Claim 43 in which R 3 R, and R 6 independently represent hydrogen halo (particularly fluoro), optionally substituted lower alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxy- methoxy, 2-carboxyethoxy, 2-carbamoylethoxy, 3-carbamoylpropoxy, 2- piperidinoethoxy, 2-(piperazin-1-yl)ethoxy, 2-(pyrrolidin-1-yl)ethoxy, 2- dimethylaminoethoxy, 2-(perhydrothiazin-1I-yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin- 1-yl)propoxy, 3-(pyrrolidin-1 -yl)propoxy, 3- dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), carbamoylmethoxy, hydroxypropyloxy, hydroxyethoxy, (3- morpholino)propoxy and (2-morpholino)etlioxy), amido (particularly acetamido and benzamido), optionally substituted carbamoyl (particularly carbamoyl, N-methyl-carbamoyl and N-phenylcarbanioyl), carboxy, nitro and amino.

48. Compounds according to Claim 43 in which R 3 R, R, and R 6 represent the following 6,7-dimethoxy, 6,7,8-trimethoxy, 6- fluoro, 6-acetamido, 7-methoxy, 6-carbamoyl, 6-(N-methylcarbamoyl), 6-(N- phenylcarbamoyl), 3-morpholinopropoxy and 2-morpholinoethoxy.

49. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents a) substituted methylene b) carbonyl c) oxygen d) a group of formula -C=NOR7 in which R7 represents H or a C 1 -4 alkyl group, e) a group of formula NR8 in which R8. represents H, an optionally substituted C1..4 alkyl group or optionally substituted phenyl, f) a group of formula 06* (CH 2 in which n is 1, 2 or 3, or g) a group of formula S(O)p in which p is 0, 1 or 2; 0600 WO 00/27822 PCT/US99/26105 154 R, represents H; R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a Ci., alkoxy group optionally substituted by one or more of the following: hydroxy, or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Re in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C 1-6 alkyl group or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from 0, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-6 alkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-6 alkyl group a C3-8 cycloalkyl group or phenyl, wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj are as defined above, i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted WO 00/27822 PCT/US99/26105 155 phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C.6 alkyl group, a C,. 6 alkoxy group or halo, providing that at least one of R3 R4 R5 and R 6 or a substituent on R, represents one of the following: a) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, b) a C1-6 alkyl group substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj are as defined above, c) a C. 6 alkoxy group optionally substituted by one or more of the following: hydroxy, or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo d) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc wherein Rb and Re are as defined above, e) a group of formula NRdRe in which Rd and Re are as defined above.

50. Compounds according to Claim 49 in which R 2 represents optionally substituted phenyl, naphthyl, optionally substituted thienyl, optionally substituted pyridyl, optionally substituted furyl, or optionally substituted pyrrolyl.

51. Compounds according Claim 49 in which R, represents 2-thienyl, 3-thienyl, 2-furyl, 3-furyl, 2-pyridyl, 3-pyridyl or 4- pyridyl each of which is optionally substituted, naphthyl and optionally mono-, di- and tri-substituted phenyl, wherein the substituents are selected from optionally substituted alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxymethoxy, 2-carboxyethoxy, 2-carbamoylethoxy, carbamoylmethoxy, WO 00/27822 WO 0027822PCT[US99/261 156 3-carbamoylpropoxy, 2-piperidino-ethoxy, 2.-(piperazin- 1-yl)ethoxy, 2- (pyrrolidin-1I-yl)ethoxy, 2-dimethylamnino-ethoxy, 2-(perhydro-thiazin-4- yl)ethoxy, 3-piperidinopropoxy, 3 -(piperazin-1I-yl)propoxy, 3-(pyrrolidin- 1 yl)-propoxy, 3-dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), lower alkyl (particularly methyl), halo (particularly fluoro and chloro), aryl (particularly phenyl), hydroxy, aryloxy (particularly phenoxy), arylalkoxy (particularly benzyloxy), di-lower-alkylamino (particularly dimethylamino), polyhalo-lower-alkyl, polyhalo-lower-alkoxy (particularly difluoromethoxy), nitro, cyano, lower-alkylthio (particularly methylthio), carboxy, lower- alkoxycarbonyl (particularly methoxycarbonyl), amido (particularly acetamnido and benzamido) and optionally substituted carbainoyl (particularly carbamnoyl, N-methylcarbamoyl, N-phenylcarbamoyl) and a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following: trifluoromethyl or nitro.

52. Compounds according to Claim 49 in which R 2 represents 4-pyridyl, 2-formnamidomethyl-4-pyridyl, 2-aminomethyl-4- pyridyl, 2-(hydroxyamidino)-4-pyridyl, 2-carbamoyl-4-pyridyl, 4-pyridyl-N- oxide, 2-chloro-4-pyridyl, 2-cyano-4-pyridyl, 5-methyl-2-furyl, 5-(2-nitro-4- trifluoromethylphenyl)fur-2-yl, phenyl, 4-methoxyphenyl, 3-methoxyphenyl, 2-methoxyphenyl, 3,4-dimethoxyphenyl, 3 ,4,5-trimethoxyphenyl, 4-(3- morpholinopropoxy)phenyl, 4-(2-morpholinoethoxy)phenyl, 4-(3-carboxy- propoxy)phenyl, 4-carboxymethoxyphenyl, 4-(3 -carbamoylpropoxy)phenyl, 4-carbamoylmethoxyphenyl, 3-(3-morpholino-propoxy)phenyl, 3-(2- morpholino-ethoxy)phenyl, 3-(3-carboxy-propoxy)phenyl, 4- carboxymethoxyphenyl, 3-(3-carbamoylpropoxy)phenyl, 3- carbamoylmethoxyphenyl, 2-hydroxyphenyl, 3-hydroxyphenyl, 4-hydroxyphenyl, 3 -hydroxy-4-methoxyphenyl, 4-hydroxy-3-. methoxyphenyl, 4-difluoromethoxyphenyl, 3-nitrophenyl, 4-nitrophenyl, di-tert-butyl-4-hydroxyphenyl, 4-methylphenyl, 4-fluorophenyl, 2- chlorophenyl, 3-chlorophenyl, 4-chiorophenyl, 2,4-dichlorophenyl, 2-chioro- 4-fluoro-2-chlorophenyl, 4-methylthiophenyl, 4-biphenylyl, WO 00/27822 WO 0027822PCTIUS99/26 105 157 3-phenoxyphenyl, 4-phenoxyphenyl, 4-benzyloxyphenyl, 4- dimethylaminophenyl, 4-diethyl-aminophenyl, 4-methoxycarbonyiphenyl, 4-carbamayiphenyl, 4-cyanophenyl, 4-N-methylcarbamoylphenyl, 4-N- phenylcarbamoylphenyl, 4-acetamido-phenyl, 4-benzamidophenyl 4- carboxyphenyl, 4-[N-(2-diethylaminoethyl)-carbamoyl]phenyl, 4-(prop- 1- enyloxy)phenyl, 3-(2-hydroxyethoxy)phenyl, 3-(N-(2-diethyl aminoethyl)- carbamoylmethoxy)phenyl, 3 -[3-(N-(2-diethylamino-ethyl)carbamoyl) propoxylphenyl, 4-(N-(2-diethylaminoethyl)carbamoyl-methoxy)phenyl, 4- [3 -(N-(2-diethylaminoethyl)-carbamoyl)propoxy]phenyl, 2-furyl, 5-[3 bis(trifluoromethyl)phenyl] -2-furyl, 3 -bromo-2-thienyl, 5-methoxy-2-furyl, 5-(2-nitro-4-trifluoromethylphenyl)-2-furyl, 3-N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl, 3-[3-(N-(2-morpholinoethyl) carbamoyl)propoxy-phenyl], 4-(N-(2-morpholinoethyl)- carbamoylmethoxy)phenyl 4-(morpholinacetamido)phenyl and 4-[3 morpholinoethyl)carbamoyl)propoxy]phenyl.

53. Compounds according to Claim 49 in which R 3 R, and R 6 independently represent hydrogen halo (particularly fluoro), optionally substituted lower alkoxy (particularly methoxy, 3- morpholinopropoxy, 2-morpholinoethoxy, 3-carboxypropoxy, carboxy- methoxy, 2-carboxyethoxy, 2-carbamoylethoxy, 3-carbamoylpropoxy, 2- piperidinoethoxy, 2-(piperazin- 1 -yl)ethoxy, 2-(pyrrolidin- 1 -yl)ethoxy, 2- dimethylaminoethoxy, 2-(perhydrothiazin- 1-yl)ethoxy, 3-piperidinopropoxy, 3-(piperazin-1I-yl)propoxy, 3-(pyrrolidin- 1-yl)propoxy, 3- dimethylaminopropoxy, 3-(perhydrothiazin-4-yl)propoxy), carbamoylmethoxy, hydroxypropyloxy, hydroxyethoxy, (3- morpholino)propoxy and (2-morpholino)ethoxy), amido (particularly acetamido and benzamido), optionally substituted carbamoyl (particularly carbamoyl, N-methyl-carbamoyl and. N-phenylcarbamoyl), carboxy, nitro and amino. 158

54. Compounds according to Claim 49 in which R 3 R, and R 6 represent the following 6,7-dimethoxy, 6,7,8-trimethoxy, 6- fluoro, 6-acetamido, 7-methoxy, 6-carbamoyl, 6-(N-methylcarbamoyl), 6-{N- phenylcarbamoyl), 3-morpholinopropoxy and 2-morpholinoethoxy. A compound of formula I as defined in Claim 1 selected from: 3-(3,4,5-trimethoxyphenyl)- 1,4-dihydroindeno[1 ,2-c]pyrazole, 3-(l ,4-dihydroindeno 1 ,2-c]pyrazol-3-yl)phenol, 3-phenyl- IH-[l1]benzothieno[3,2-c]pyrazole, 3 -(2-thienyl)- IH-l I]benzothieno[3,2-clpyrazole, 3-phenyl- lH-[1)benzothieno[3,2-c]pyrazole 4-oxide, 3-phenyl-lIH-[ I ]benzothieno[3,2-cjpyrazole 4,4-dioxide, 3-(2-thienyl)- IH-[ I ]benzothieno [3,2-clpyrazole, 3-phenylindeno[ 1,2-c]pyrazol-4(lH)-one oxime, 3-(3,4-dimethoxyphenyl)indeno[l ,2-c]pyrazol-4(lH)-one oxime, 3-(4-methylphenyl)indeno[ 1,2-c]pyrazol-4( lm-one oxime, 3-(2-thienyl)indeno[1 ,2-c]pyrazol-4(lH)-one, 3-phenyl- IH-benzofuro[3 ,2-c]pyrazole, 1 ,4-dihydro-3-phenylpyrazolo[4,3-b]indole, 20 1 ,4-dihydro-4-methyl-3-phenylpyrazolo[4,3-b]indole, 4,4-dimethyl-3-phenyl- 1,4-dihydroindeno[ 1,2-cjpyrazole, 4-(l ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzoic acid, methyl 4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzoate, ,4-dihydroindeno[1,2-c]pyrazol-3-yl)acetanilide, ,4-dihydroindeno[ I ,2-clpyrazol-3-yl)-3-morpholinopropionanilide, 4' ,4-dihydr'oindeno[ 1,2-clpyrazol-3-yl)morpholinoacetanilide, ,4-dihydroindeno[1I,2-c]pyrazol-3-yl)benzanilide, N-(3-phenyl- 1,4-dihydroindeno[l ,2-clpyrazol-6-yl)acetamide, 3 -morpholino-N-(3-phenyl- I ,4-dihydroindeno[ 1 ,2-clpyrazol-6-yl)propionamide, 30 N-(3-phenyl-1 ,4-dihydroindeno[1 ,2-c~pyrazol-6-yl)benzanilide, 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-methyl-4-(1 ,4-dihydroindeno 1 ,2-c]pyrazol-3-yl)benzamide, WO 00/27822 WO 0027822PCTILJS99I26105 159 1,4-dihydroindeno[ [1,2-c~pyrazol-3-yl)benzanilide, N -(2-diethylaminoethyl)-4-(1I,4-dihydroindeno[ 1,2-cjlpyrazol-3-yl)benzamide, N-(2-morpholinoethyl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3 -yI)benzamide, 4-(1I,4-dihydroindeno[l ,2-clpyrazol-3-yl)phenol, 3-[3-(2-morpholinoethoxy)phenyl]- 1,4-dihydroindeno[ 1,2-c]pyrazole, 3 -(2-thienyl)- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-ol, 6-(2-morpholinoethoxy)-3-(2-thienyl)- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole, 3-[3 -(2-hydroxyethoxy)phenyl]- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole, 3-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenoxyacetic acid, ethyl 3-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenoxyacetate, 3 ,4-dihydroindeno [1,2-c]pyrazol-3-yl)phenoxyacetamnide, N-(2-diethylaminoethyl)-3-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)phenoxyacetamide, N-(2-morpholinoethyl)-3 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenoxyacet am-ide, 4- ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenoxy~butyric acid, ethyl 4- 1,4-dihydroindeno[ 1,2-clpyrazol-3-yl)phenoxy} butyrate, 4- {3-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenoxy}butyrainide, N-(2-diethylarninoethyl)-4- {3-(l1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenoxy} butyramide, N-(2-morpholinoethyl)-4- 1,4-dihydroindeno [1 ,2-c]pyrazol-3-yl)phenoxy} butyramide, 3-(2-thienyl)- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole-6-carboxamide, N-methyl-3-(2-thienyl)- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole-6-carboxamide, N-(2-morpholinoethyl)-3 -phenyl- 1 ,4-dihydroindeno[ 1 ,2-c~pyrazole-6-carboxamide, 3-(2-thienyl)- I ,4-dihydroindeno[ 1 ,2-c]pyrazole-6-carboxanilide; N-(3-phenyl- 1 ,4-dihydroindeno[ 1 ,2-clpyrazol-6-yl)acetamide, 3-phenyl- 1,4-dihydroindeno [1 ,2-c]pyrazol-6-ylamine, 3-(4-nitrophenyl)- 1 ,4-dihydroindeno[ 1 ,2-clpyrazole, 4-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)aniline, 4-(4,5-dihydro-l1H-benzo[glindazol-3-yl)pyridine 1-oxide, 3-(2-chloro-4-pyridyl)-4,5-dihydro-l1H-benzo~g]indazole, 4-(4,5-dihydro- 1H-benzo[g]indazol-3-yl)-2-pyridinecarbonitrile, WO 00/27822 WO 0027822PCTIUS99/261 160 4-(4,5-dihydro- 1H-benzo[g]indazol-3 -yl)-2-pyridinecarboxamide oxime, 4-(4,5-dihydro- IH-benzo[g]indazol-3-yl)-2-pyridinecarboxamide, [4-(4,5-dihydro- 1 H-benzo[glindazol-3-yI)-2-pyridyl]methyl} ammonium chloride, N- {[4-(4,5-dihydro- 1H-benzo[g] -indazol-3-yl)-2-pyridyllmethyl} formainide, 1,4-dihydroindeno[1I,2-c]pyrazol-3-yl)phenoxy]ethanol, 2-morpholinoethyl 4-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)benzoate, 3-(3-nitrophenyl)-1,4-dihydroindeno[1 ,2-c]pyrazole, 3-(4-thiomethoxyphenyl)- 1,4-dihydroindeno[ 1,2-c]pyrazole, 3-(2-naphthyl)- 1,4-dihydroindeno 1 ,2-c]pyrazole, 3-(4-difluorornethoxyphenyl)- 1,4-dihydroindeno [1 ,2-c]pyrazole, 3-(4-acetanmidophenyl)-4,5-dihydro-2H-benzg]indazole, 3-(4-bromo-2-thienyl)-4,5-dihydroindeno[ 1,2-cjpyrazole, 3-(4-benzyloxyphenyl)-4,5-dihydro-2H-benz[g]indazole, 6,7-dimethoxy-3-(3-phenoxyphenyl)- 1,4-dihydroindeno- [1 ,2-c]pyrazole, 3 -[4-(5-trifluoromethyl-2-pyridyloxy)phenyl]- 1,4-dihydroindeno[1I,2-c]pyrazole, 6,7,8-trimethoxy-3-(2,3,4-trimethoxyphenyl)- 1,4-dihydroindeno[ 1,2-c]pyrazole, 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yI)-2-hydroxymethyl)phenol, 2-methoxy-5-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenol, 2-chloro-4-(1 ,4-dihydroindeno[1,2-c]pyrazol-3-yl)phenol, 2-methoxy-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenol, 3-chloro-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenol, 1,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)phenoxy]acetamide, 4 ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)diethylamino-acetanilide, IH-[ I ]benzothieno[3 ,2-c]pyrazol-3-yl)benzamide, 3-(4-aminophenyl)- 1H-[l1]benzothieno [3,2-cjpyrazole, 3-(4-methoxyphenyl)- IH-benzothieno[3,2-c]pyrazole, 3-(4-hydroxyphenyl)-l1H-[ I]benzothieno[3 ,2-c]pyrazole, N-(3-phenyl-1 ,4-dihydroindeno[1 ,2-c]pyrazol-6-yl)benzamide, N-(2-morpholinoethyl)-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, 4-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzonitrile, 7 -methoxy-3-(4-methylsulphonylphenyl)-4,5-dihylro-2H-benz[g]indazole, 4-methyl-3-phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-4-ol, WO 00/27822 WO 0027822PCTIUS99/26 105 161 N-[2-(N,N-diethylamino)ethyl]-4-( 1,4-dihydroindeno[1I,2-c]pyrazol-3-yl)benzamide, N- [2-(N,N-dimethylamino)ethyl]-4-( 1,4-dihydroindeno[1I,2-c]pyrazol-3- yl)benzainide, N-[2-(N,N-dipropylaniino)ethyl]-4-(1,4-dihydroindeno[1 ,2-c]pyrazol-3- yI)benzamide, N-[2-(N,N-di-isopropylamino)ethyll-4-(1,4-dihydroindeno[1 ,2-c]pyrazol-3- yl)benzamide, N-[3-(N,N-diethylamnino)propyl]-4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3- yl)benzamide, N-[3-(N,N-dimethylamino)propyl]-4-( 1,4-dihydroindeno[1I,2-c]pyrazol-3- yl)benzamide, N- [3-(N,N-dipropylamino)propyl]-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzamide, N-[3-(N,N-di-isopropylamino)propyl]-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3- yl)benzamide, N-(2-Piperidinoethyl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-(2-Piperidinopropyl)-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)benzamide, N-(2-Morpholinoethyl)-4-( 1,4-dihydroindeno[ 1,2-cjpyrazol-3-yl)benzamide, N-(2-Morpholinopropyl)-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzainide, N-[2-(Piperazin- 1 -yl)ethyl]-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamide, N-[3-(Piperazin- 1 -yl)propyl]-4-( 1,4-dihydroindeno[1 ,2-c]pyrazol-3-yl)benzamide, N-[2-(Pyrrolidin- 1 -yl)ethyl]-4-(1 ,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-[3-(Pyrrolidin- 1 -yl)propyl]-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)benzainide, N-[2-(4-Methylpiperazin- I -yl)ethyl]-4-(1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzamide, N-[3-(4-Methylpiperazin- 1 -yl)propyl]-4-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzaniide, N-[2-(Thiomorpholin- 1 -yl)ethyl]-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzamide, N-[3-(Thiomorpholin- 1-yl)propyl]-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3- yl)benzamide, WO 00/27822 WO 0027822PCTIUS99/26105 162 N-[2-(Homopiperazin- 1 -yl)ethyl]-4-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzamide, N- [3-(Homopiperazin- 1 -yl)propylj-4-( 1,4-dihydroindeno[ [1,2-c]pyrazol-3- yl)benzamide, N-[2-(Perhydroazepin- 1 -yl)ethyl] -4-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzamide, N-[3-(Perhydroazepin-1 -yl)propylj-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzamide, N-Isopropyl-4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)benzamide, N-But-2-yl-4-( 1,4-dihydroindeno[ [1,2-c]pyrazol-3 -yl)benzamide, N-Methyl-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-Ethyl-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-Pentyl-4-(1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-(2-Bromoethyl)-4-(1I,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)benzamide, N-(3 ,3 ,3-trifluoroprop- I 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamnide, N-(Cyclopropylmethyl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-Cyclopentyl-4-(1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamnide, N-(Cyclohexylmethyl)-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamide, N-(2-Chlorocyclopentyl)-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)benzaxnide, N-[3-(N,N-dimethylamino)-2,2-dimethylpropyl-4-(1 ,4-dihydroindeno[ 1,2- c]pyrazol-3-yl)benzamide, N-[3-(2-Methylpiperidin- 1 -yl)propyl]-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3 yl)benzamide, N-(3-Methylbut-2-yl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-[2-(Pyrrolidin- 1 -yl)ethyl]-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamide, N-[3-(N,N-Dimethylamino)prop-2-yl]-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzamide, N-(2-Hexyl)-4-(1 ,4-dihydroindeno [1 ,2-c]pyrazol-3-yl)benzamide, N-tert-Butyl-4-( 1,4-dihydroindeno[ 1,2:,c~pyrazol-3-y1)benzamide, N-[7-(N,N-Dimethylamino)heptyl]-4-( 1,4-dihydroindeno[ 1,2-clpyrazol-3- yl)benzamide, N-(2-Methylbut-2-yl)-4-(1 ,4-dihydroindeno[1I,2-cjpyrazol-3-yl)benzamide, WO 00/27822 WO 0027822PCTIUS99/26 105 163 N-(2-Pentyl)-4-( 1,4-dihydroindeno 1,2-c]pyrazol-3 -yl)benzamide, N-sec-Butyl-4-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)benzamide, N-(3 ,3-Dimethylbutyl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-(2,2,3 ,3,3-Pentafluoropropyl)-4-( 1,4-dihydroindeno( 1,2-c]pyrazol-3- yl)benzamide, N-(2,5-Dichloropentyl)-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-(2,2-Difluoroethyl)-4-(1I,4-dihydroindeno[ I ,2-c]pyrazol-3-yl)benzarnide, N-(2-Chloroethyl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3 -yl)berizamide, N-[2-(N,N-dimethylamino)propyl]-4-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3 yl)benzamide, N-(3 -Morpholinopropyl)-4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)benzamide, N-[3-(Pyrrolidin- 1 -yl)propyl]-4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3-yl)benzamide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)piperidinoacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-4-methylpiperazin- 1-ylacetaniilide, (1 ,4-Dihydroindeno[ 1,2-clpyrazol-3-y1)-4-methylhomopiperazin-1I-ylacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)piperazin- 1-ylacetanilide, (1 ,4-Dihydroindeno[ 1,2-cjpyrazol-3-yl)homopiperazin-1I-ylacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)dipropylaminoacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-y1)dimethylaminoacetanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)fluoroacetanilide, (1 ,4-Dihydroindeno[1I,2-c]pyrazol-3-yl)-3,5-difluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-4-fluorobenzylanilide, (1 ,4-Dihydroindeno[1,2-c]pyrazol-3-yl)-2-fluorobenzylanilide, (1 ,4-Dihydroindeno [1 ,2-c]pyrazol-3-yl)-3-fluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)-2,4-difluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-2,5-difluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yI)-2,3-difluorobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-4-nitrobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol:3 -yl)-3 -nitrobenzylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-3,3,3-trifluoropropananilide, (1 ,4-Dihydroindeno [1 ,2-c]pyrazol-3-yl)isobutananilide, (1 ,4-Dihydroindeno[1I,2-c]pyrazol-3-yl)isopentananilide, WO 00/27822 WO 0027822PCTIUS99/261 164 (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-2-methylbutananilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-2-methylpentanaflilide, (1 ,4-Dihydroindeno[1I,2-c]pyx-azol-3-yl)-2-ethylbutanaflilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)neopentylanilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-4,4-dimethylpeltalafilide, (1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)cyclohexanaflilide, (1 ,4-Dihydroindeno [1 ,2-c]pyrazol-3-yl)trifluoroacetaflilide, (1 ,4-Dihydroindeno[1I,2-c]pyrazol-3 -yl)pentafluoropropananilide, Fluoro-N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)acetamide, 3 ,5-Difluoro-N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamide, 4-Fluoro-N-(3 -Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamide, 2-Fluoro-N-(3-Phenyl-1I,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamide, 3-Fluoro-N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamide, 2,4-Difluoro-N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamnide, 2,3-Difluoro-N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-clpyrazol-6-yl)benzylamide, 2,5-Difluoro-N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)benzylamide, 4-Nitro-N-(3-Phenyl-1I,4-dihydroindeno[1I,2-c]pyrazol-6-yl)benzylamide, 3-Nitro-N-(3-Phenyl-1 ,4-dihydroindeno[l ,2-c]pyrazol-6-yl)benzylamide, 3,3 ,3-Trifluoro-N-(3 -Phenyl- 1,4-dihydroindeno[1I,2-c]pyrazol-6-yl)propanamide, N-(3 -Phenyl- 1,4-dihydroindeno [1 ,2-c]pyrazol-6-yl)isobutanamide, N-(3 -Phenyl- 1,4-dihydroindeno [1 ,2-c~jpyrazol-6-y)isopentanamnide, 2-Methyl-N-(3-phenyl- 1,4-dihydroindeno [1 ,2-c]pyrazol-6-yl)butanamide, 2-Methyl-N-(3-phenyl- 1 ,4-dihydroindeno[1 ,2-c]pyrazol-6-yl)pentanamide, 2-Ethyl-N-(3-phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)butanamide, N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)neopentanamide, 4,4-Dimethyl-N-(3-phenyl- 1,4-dihydroindeno[1I,2-c]pyrazol-6-yl)pentanamnide, N-(3-Phenyl- 1,4-dihydroindeno[ 1,2-cllpyrazol-6-yl)cyclohexanecarboxamide, Trifluoro-N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-6-yl)acetamide, Pentafluoro-N-(3-Phenyl- 1 ,4-dihydroindeno[ 1,2-c]pyrazol-6-yl)propanamide, (1 ,4-Dihydroindeno[1 ,2-c]pyrazol-3-yl)acetanilide, 2-Hydroxy-N-(2-morpholinoethyl)-5-( 1,4-dihyclroindeno[ 1,2-c]pyrazol-3- yl)benzarnide, WO 00/27822 WO 0027822PCTIUS99/261 165 2-Hydroxy-N-(2-morpholinopropyl)-4-(1I,4-dihydroinderio[ 1 ,2-c]pyrazol-3- yl)benzamide, 2-Hydroxy-N-[2-(pyrrolidin- 1 -yl)ethyl]-5-( 1,4-dihydroindeno[ [1,2-clpyrazol-3- yl)benzamide, 2-Hydroxy-N-[3-(pyrrolidin- 1 -yl)propyl]-5-( 1,4-dihydroindeno[ [1,2-cllpyrazol-3- yI)benzamide, N-[2-(N,N-Diethylamino)ethyl]-5-(1I,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)-2- hydroxybenzamide, 1,4-dihydroindeno[ [1,2-c]pyrazol-3-yl)-2- hydroxybenzamide, 2-Hydroxy-N-[2-(N,N-dimethylamino)ethyl]-5-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3 yl)benzamide, 2-Hydroxy-N-[3-(N,N-dimethylamino)propyl] 1,4-dihydroindeno[lI,2-c]pyrazol- 3-yl)benzamnide, 2-Hydroxy-N- [2-(N,N-dipropylamino)ethyl]-5-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3- yI)benzamide, 2-Hydroxy-N-[2.-(N,N-di-isopropylamino)ethyl]-5-( 1,4-dihydroindeno[ 1,2- c]pyrazol-3-yl)benzamnide, 2-Hydroxy-N- [3 -(N,N-dipropylamino)propyl]-5-(1I,4-dihydroindeno[ 1,2-c]pyrazol- 3-yl)benzamnide, 2-Hydroxy-N- [3-(N,N-di-isopropylamino)propyl]-5 ,4-dihydroindeno[ 1,2- clpyrazol-3-yl)benzamide, 2-Hydroxy-N-(2-piperidinoethyl)-5-( 1,4-dihydroindeno[ 1 ,2-clpyrazol-3- yl)benzamide, 2-Hydroxy-N-(2-piperidinopropyl)-5-(1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3 yl)benzamide, 2-Hych-oxy-N-[2-(piperazin- 1 -yl)ethyl]-5-( 1,4-dihydroindeno 1,2-c]pyrazol-3 yl)benzamide, 2-Hydroxy-N-[3 -(piperazin- 1 -yl)propyl]-5 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzamide, 2-Hydroxy-N- [2-(4-methylpiperazin- 1 -yl)ethyl]-5 ,4-dihydroindeno[ 1,2- c]pyrazol-3-yl)benzainide, WO 00/27822 WO 0027822PCTIUS99/26105 166 2-Hydroxy-N-[3-(4-methylpiperazin- 1 -yl)propyl]-5-( 1,4-dihydroindeno[ 1,2- clpyrazol-3-yl)benzamnide, 2-Hydroxy-N-[2-(thiomorpholin- 1 -yl)ethyl]-5-(1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3 yl)benzamide, 2-Hydroxy-N-[3 -(thiomorpholin- 1 -yl)propyl] 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3 yl)benzamide, 2-H-ydroxy-N-[2-(Homopiperazin- 1 -yl)ethyl]-5-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)benzarnide, 2-Hydroxy-N-[3 -(Homopiperazin- 1 -yl)propyl]-5-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol- 3-yl)benzamide, 2-Hydroxy-N- [2-(Perhydroazepin- I -yl)ethyi]-5-( 1,4-dihydroindeno[ I ,2-c]pyrazol-3 yI)benzamide, 2-Hydroxy-N-[3 -(Perhydroazepin- I -yl)propyl]-5-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol- 3-yl)benzamide, 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)-N-(2-morphilinoethyl)aniline, 1,4-Dihydroindeno[1I,2-c]pyrazol-3-yI)-N-(2-morphilinopropyl)aniline, 4-(1 4 -Dihydroindeno[ 1,2-c]pyrazol-3-yl)-N-(2-piperidinoethyl)aniline, 1,4-Dihydroindeno[ 1 2 -clpyrazol-3-yl)-N-(2-piperidinopropyl)aniline, 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)-N-[2-(thiomorphilinl -yl)ethyl] aniline, 4-(1 ,4-Dihydroindeno 1 ,2-c]pyrazol-3 -yl)-N-[2-(thiomorphilin 1 -yl)propyl] aniline, 1,4-Dihydroindeno[ 1 2 -c]pyrazol-3-yl)-N-[2-(piperazin- 1 -yl)ethyl] aniline, 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)-N-[2-(piperazin- I -yl)propyl] aniline, 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)-N-[2-(4-methylpiperazin- 1 1)ethyl] aniline, 4-(1 ,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)-N-[2-(4-methylpiperazin- 1- yl)propyl] aniline, N-[2-(N,N-Diethylaniino)ethyl]-4-(1 ,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)aniline, N-[3-(N,N-Diethylamino)propyl]-4.( 1 ,4-Dihydroindeno[ [1,2-c]pyrazol-3-yl)aniline, 4-(1 ,4-Dihydroindeno[ I 2 -c]pyrazo1-3-y1)-N- [2-(N,N-dipropylamino)ethy1] aniline, 1,4-Dihydroindeno 1 2 -c]pyrazo1-3 [3 -(N,N-dipropylamino)propyl] aniline, 4-(1 ,4-Dihydroindeno[ 1 2 -c]pyrazol-3 -y)-N-[2-(N,N-dimethy1amino)ethyl] aniline, 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -(N,N-dimethylamino)propyl) aniline, WO 00/27822 PCTIUS99/261 167 Methyl 4-(6-Acetamido- 1,4-Dihydroindeno[ 1, 2 -c]pyrazol-3-yl)benzoate, N-(3-Methoxypropyl)-4-(1 ,4-dihydroindeno[ 1 2 -c]pyrazol-3-yl)benzarnide, 1,4-Dihydroindeno[ l, 2 -c]pyrazol-3-yl)-N-(4-Nitrophenyl)benzamide, N-(3 -Phenyl- 1 ,4-dihydroindeno[ 1 2 -c]pyrazol-6-yl)morpholinoacetamide, N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 2 -c]pyrazol-6-y)morphoinoacetmide, N-(3 -Phenyl-l 4 -dihydroindeno[ 1,2-c]pyrazol.6yl)piperidinoacetamide N-(3 -Phenyl- 1 ,4-dihydroindeno[ 1, 2 -c]pyrazol-6-yl)thiomorpholinoacetamde, N-(3-Phenyl- 1 ,4-dihydroindeno[ 1 2 -c]pyrazol-6-yl)-4-methylpiperazin- 1 ylacetatnide, N-(3-Phenyl- 1 ,4-dihydroindeno 1, 2 -c~jpyrazol-6-yl)piperazin- 1 -ylacetamide, N-(3 -Phenyl- 1 ,4-dihydroindeno 1, 2 -c~pyrazol-6-yl)pyrrolidin- 1 -ylacetamide, 2 -(N,N-Diethylamino)-N-(3-phenylp 1 ,4-dihydroindeno[ 1 2 -c]pyrazol-6- yl)acetamide, N-(3 -Phenyl- 1,4-dihydroindeno[ l, 2 -c]pyrazol-6-yl)-2-(dimethylamino)acetmide, N-(3-Phenyl- 1 ,4-dihydroindeno[ l, 2 -c]pyrazol-6-yl)-2-(dipropylamino)acetamide, 4-(6-Amino- 1,4-dihydroindeno[ l, 2 -c]pyrazol-3-yl)-N-[2-(NNdiethylamino)ethyl]j benzamide, 3-[3 2 -Morpholinoethoxy)phenyl]y 1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3 3 -(2-Morpholinoethoxy)phenyl]- 1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3 3 -Morpholinopropoxy)pheny]-. 1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3 3 2 -Piperidinoethoxy)phenyl]y 1 ,4-dihydroindeno 1,2c]pyrazole, 3 3 3 -Piperidinopropoxy)phenyl]y 1 ,4-dihydroindeno[ 1 ,2c]pyrazole, 3-1{3 -[2-(Piperazin- 1 -yl)ethoxy]phenyl} 1,4-dihydroindeno[ 1 ,2c]pyrazole, 3- 3 3 -(Piperazin- 1 -yl)propoxy]phenyl} 1,4-dihydroindeno[ 1 ,2cjpyrazole, 3- f{ 3 -1 2 -(4-Methylpiperazin- 1 -yl)ethoxy]phenyl} 1,4-dihydroindeno 1, 2 c]pyrazole, 3- 3 3 4 -Methylpiperazin- 1 -yl)propoxy]phenyl} 1,4-dihydroindeno[ 1 2 c]pyrazole, 3- 3 -[2-(Homopiperazin- 1 -yl)ethoxy]phenyl} 1,4-dihydroindeno[ 1 2 cjpyrazole, 3- 3 -13-(Homopiperazin- 1 -yl)propoxy]phenyl} 1,4-dihydroindeno[ 1 ,2c]pyrazole, 3- 3 -1 2 -(4-Methylhomopiperazin- 1 -yl)ethoxy]phenyl} 1,4- dihydroindeno[ 1 ,2c]pyrazole, 3- (3 3 4 -Methylhomopiperazin- 1 -yl)propoxy]phenyl} 1,4- dihydroindeno[ 1,2c]pyrazole, WO 00/27822 WO 0027822PCTIUS99/26 105 168 3- {3-[2-(iN,N-Diethylamino)ethoxy]phenyl} 1,4-dihydroindeno[ 1,2c]pyrazole, 3- {3-[3-(N,N-Diethylamino)propoxy]phenyl} 1,4-dihydroindeno[ 1 ,2c]pyrazole, 3- {3 -[2-(N,N-Dimethylamino)ethoxy]phenyl} 1,4-dihydroindeno[ 1 ,2c]pyrazole, 3- [3-(N,N-Dimethylamino)propoxy]phenyl} -1 ,4-dihydroindeno[ [1,2c]pyrazole, 3- {3-[2-(N,N-Dipropylamino)ethoxy]phenyl} -1 ,4-dihydroindeno[l1,2c]pyrazole, and 3- {3-[3-(N,N-Dipropylamino)propoxy]phenyl} -1 ,4-dihydroindeno[ 1,2c]pyrazole, and pharmaceutically acceptable salts thereof and tautomners thereof.

56. A compound selected from: R 3 R2R. X=(CH2)n TABLE I R3 R4R5 R6 N R H 1 N 5 N 2 H 13-N02 6,7-(OMe)2 13-OPh H I4-SMe H 1N H 1 -3-OH,4-OMe H 14-OH,3-OMe H 1-O 16,7-(OMe)2 1 4-NMe2 WO 00/27822 WO 0027822PCT/US99/26105 R3 R4 R5R6 N Rx H1 3,4- 6,7,8-(OMe)3 12,3,4-(OMe)3 H 14-OCHIF2 H 14-OH, 3,5-(But )2 H 2 4-NHAc H 2 4-OCH2 CH=CH2 H 2 4-NIEt2 H 2 4-N02 H 2 4-OCH2Ph H 2 4-CN H 2 2-Cl, 5-N02 and pharmaceutically acceptable salts thereof and tautomers thereof.

57. A compound selected from: R3 2 R) 1 X=(CH2)n TABLE 2 R3 R4R5 R 6 N R,) 6-AcNH I WO 00/27822 WO 0027822PCT/US99/261 R3 R4R5 R6 N R2 I CF 3 H 1Br 7-OMe 2 z Me H 2 O M and pharmaceutically acceptable salts thereof and tautomers thereof.

58. A compound selected from: R3 R34RR x R2 H c~o NO N I0 C F and pharmaceutically acceptable salts thereof and tautomers thereof. 171

59. A pharmaceutical composition comprising a compound of formula I as defined in Claim 1 in conjunction with a pharmaceutically acceptable diluent or carrier. A compound of formula I as defined in Claim 1 when used as a medicament.

61. A compound of formula I as defined in Claim 1 when used as a medicament for inhibiting protein kinase activity.

62. The use of a compound of formula I as defined in Claim 1 in the manufacture of a medicament for use in inhibiting protein kinase activity.

63. The method of Claim 1 wherein said compound is in an enantiomeric form, is in a mixture with one or more other said compounds, or is both in an enantiomeric form and in a mixture with one or more other said compounds.

64. The method of Claim 1 wherein said protein kinase is a serine kinase. S 65. The method of Claim 1 wherein said protein kinase is a threonine kinase.

66. The method of Claim 2 wherein said tyrosine kinase is KDR.

67. A compound of formula I as defined in Claim 1 selected from: Dihydroxy 4-(4H-indeno-[1,2-c]-pyrazol-3-yl)phenylborane 4-(1H-[1]Benzothieno[3,2-c]pyrazol-3-yl)benzaldehyde 4-(1 H-[1 ]Benzothieno[3,2-c]pyrazol-3-yl)-N-[3-(imidazol- 1 -yl)propyl] benzylamine trihydrochloride 0Methyl 4-(4-oxo-1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzoate 4-(1,4-dihydroindeno[1,2-c]pyrazol-3-yl)benzamide oxime 3-{4-[(2-diethylaminoethyl)aminomethyl]phenyl}-1,4-dihydroindeno[1,2-c] pyrazole trihydrochloride N-[4-(1,4-Dihydroindeno[1,2-c]pyrazol-3-yl)phenyl]benzenesulphonamide WO 00/27822 WO 0027822PCT/US99/26 105 172 N-(2-Morpholinoethyl)-4 '-dihydroindeno[ [1,2-.c]pyrazol-3 -ylaniline dihydrochioride 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-6-yl)-2-morpholinoacetamide N-(2-Morpholinoethyl)-3-phenyl- 1 ,4-dihyd-oindeno 1 2 -c]pyrazol-6-ylamine trihydrochioride 4 -Acetyl- 1,4-dihydroindeno[ [1,2-c]pyrazol-3 -yl)acetanilide 3 [4-(2-morpholinoethoxy)phenyl]- 1 ,4-dihydroindeno[ 1 2 -c]pyrazole 3 1 ,2,3,4-Tetraazol-5 -yl)-4-pyridyl]-4,5-dihydro-2H- benzo[g]indazole 3 -(4-Isocyanatophenyl)- 1 ,4-dihydroindeno[ 1 ,2-c]pyrazole, 2-(Diethylamnino)ethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3- yl)phenyl]carbamate 2-Morpholinoethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3- yl)phenyl]carbamate 3-(Dibenzylamino)propyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3- yl)phenyl]carbamate 2-[Ethyl(2-hydroxyethyl)amino] ethyl 1,4-dihydroindeno[ 1,2-c]pyrazol- 3-yl)phenyl]carbamate 2-fj[2-(Dimethylamino)ethyl](methyl)amino]ethyl 1,4- dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]carbamnate 1 -Methyl-2-propoxyethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3 yl)phenyl]carbamate 1 -Methyltetrahydro- 1H-2-pyrrolyl)ethyl 1,4-dihydroindeno 1,2- c]pyrazol-3-yl)phenyl]carbamate 2-[2-(Dimethylamino)ethoxy] ethyl 1,4-dihydroindeno[ 1,2-c]pyrazol-3- yl)phenyl]carbainate 2-(Diethylamino)-l1-methylethyl 1,4-dihydroindeno[ 1, 2 -c]pyrazol-3- yl)phenyl]carbamate N-[2-(Diethylamino)ethyl]-N'- 1,4-dihydroindeno[ 1,2-c]pyrazol-3- yl)phenyl]urea ,4-Dihydroindeno 1 2 -c]pyrazol-3-yl)phenyl]-NV-(2- morpholinoethyl)urea WO 00/27822PC/S/610 PCT/US99/26105 173 Ni 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)phenyl]- 1 pipecri dinecarboxamide 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N'-[2-(dimethylamino)- 1 -methylethyl]urea 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)phenyl] -N'-tetrahydro-2- fiiranylmethylurea 1,4-Dihydroindeno[ [1,2-c]pyrazol-3 -yl)phenyl]-AV-(2-furylmethyl)urea 1,3-Benzodioxol-5-ylmethyl)>N'-[4-( 1,4-dihydroindeno[ 1 ,2-clpyrazol-3 yl)phenyl]urea N-Cyclobuty1-A,"-[4-( 1,4-dihydroindeno 1,2-clpyrazol-3-yl)phenyl] N-[4-(1I,4-Dihydroindeno[ 1,2-c]pyrazo1-3-y1)pheny]-AM-(2- piperidinoethyl)urea urea N-B enzyl-N'-[4-( 1,4-dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]urea N-[4-(Diethylamino)butyl]-'-[4-( 1 ,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)phenyl]urea 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N'-[2-(2- thienyl)ethyl]urea N-[3-(Diethylamino)propyl]-N'-[4-( 1,4-dihydroindeno[ 1 ,2-c]pyrazol-3- yl)phenyl]urea ,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenyl]-N'-[( 1 -ethyltetrahydro- 1 H-2-pyrrolyl)methyl]urea N-(2,5-Difluorobenzyl)-N-[4-( 1,4-dihydroindenoE 1 ,2-c]pyrazol-3- yl)phenyl]urea 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N'-[2-(2- hydroxyethoxy)ethyl] urea AT[4-( 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)phenyl]-AP-[2-hydroxy- 1 (hydroxymethyl)ethyl]urea 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenyl]-N'-(2,3- dihydroxypropyl)urea Ni 1,4-Dihydroindeno [1 2 -c]pyrazol-3-yl)phenyl]-4-(2-pyridyl)- 1- piperazinecarboxamide WO 00/27822 WO 0027822PCTIUS99/26105 174 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N-[3- (dimethylamino)propyll -N-methylurea Ni -[4-(1I,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenyl]- 1 -azetanecarboxamide NI 1,4-Dihydroindeno[ I ,2-c]pyrazol-3 -yi)phenyl]-4-(4-fluorophenyi)- 1 piperazinecarboxamide N-Benzyi-N'-[4-(1 ,4-dihydroindeno [1 ,2-c]pyrazoi-3-yl)phenyl]-N- methylurea 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yl)phenyl]-N-ethyl-N-(2- hydroxyethyl)urea NI -[4-(1I,4-Dihydroindeno[ 1 ,2-c]pyrazol-3-yl)phenyl]-4-(2-methoxyphenyl)- 1 -piperazinecarboxamide 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yi)phenyl]-N-[2- (dimethylamino)ethyl]-N-methylurea Ni ,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yi)phenyl]-4-methyl- 1- piperazinecarboxamide Ni 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yi)phenyl]-4-(4-hydroxyphenyi)- I -piperazinecarboxamide Ni 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yl)phenyl]-4-[(E)-3-pheriyl-2- prop enyl]- 1 -pip erazinecarboxamide NI 1,4-Dihydroindeno[ 1 ,2-cjpyrazol-3-yl)phenyl]-4-phenyl- I1- piperazinecarboxamide 1,4-Dihydroindeno[ 1,2-c]pyrazol-3 -yi)phenyl]-N,N-di(2- methoxyethyl)urea 1,4-Dihydroindeno[1I,2-c]pyrazol-3 -yi)phenyl]-N-(2,3- dihydroxypropyl)-N-methylurea N,N-di[2-(Diethylamino)ethyl]-'-14-(1I,4-dihydroindeno[ 1 ,2-c]pyrazol-3 yI)phenylllurea 1,4-Dihydroindeno[1 ,2-c]pyrazol-3-yl)phenyl]-N-(2- pyridylmethyl)urea 1,4-Dihydroindeno[ 1 ,2-c]pyrazol-3 -yi)phenyl]-N'-(3- pyridyimethyl)urea WO 00/27822 WO 0027822PCT[US99/261 175 N- 1,4-Dihydroindeno[ 1,2-c]pyrazo1-3-yl)pheny]-AM-(4- pyridylmethyl)urea 1,4-Dihydroindeno 1,2-c]pyrazol-3 -yl)phenyl]-N'-(2- hydroxyethyl)urea 1,4-Dihydroindeno[ 1,2-c]pyrazol-3-yl)phenyl]-N- [7- (dimethylamino)heptyl]urea.

68. A compound selected from: R 1 =H R2 X R3,R4,R5,R6 4-(Ph)PHENYL CH, H 4-(OPh)PHENYL CH 2 H 4-(SO 2 Me)PHENYL (OH 2 2 R4 MeO 2 H),4-(OH)PHENYL CH 2 H 2 ),4-(OH)PHENYL CH 2 H 3-(OCH 2 C0NH 2 )PHENYL OH 2 H 4-(OCH 2 Ph)PHE NYL OH 2 R4,R5 (MeO) 2 4-(OCH 2 Ph)PHENYL CH 2 R5 MeG 4-(OH)PHENYL CH 2 R4, R5 (MeG) 2 3-(CONH(CH 2 2 MOR).4- OH 2 H (OH))PHENYL 3-(CONH(CH 2 2 NEt 2 CH 2 H (OH)PHENYL 4-(Br)PHENYL OH 2 H 4-(OH)PHENYL OH 2 R4 MeG 4-(CONH(CH 2 3 NEt 2 OH 2 H PHENYL 4-(CONH(CH 2 2 OMe) CH 2 H PHENYL 4-(CONH(4-NO 2 Ph)) OH 2 H PHENYL 4-(OH)PHENYL OH 2 R5 MeG 4-(CONH(CH 2 2 NEt 2 OH 2 R4 NH 2 WO 00/27822 WO 0027822PCTJUS99/261 PHENYL 4-(Br)PHENYL S H 4-(OH)PHENYL CH 2 R3 O(CH 2 2 OMe 4-(SO 2 NH(CH 2 2 MOR) OH 2 H PHENYL 4-(SO 2 NH(CH 2 2 oMe) CH 2 H PHENYL 3-(CH 2 NMe 2 HH PHENYLCHH 4-(OH)PHENYL CH 2 R3=0(0H 2 2 MOR 4-(SO 2 NH(CH 2 2 NEt 2 OH 2 H PHENYL 4-(OCH 2 C0NH 2 OH 2 R3=0(H 2 2 OMe PHENYL 4-(OH)PHENYL CH 2 R4=(OH), ___R5=0(H 2 2 OMe 4-(OH)PHENYL CH 2 R4=0(CH 2 2 OMe 4-(CONH(CH 2 2 NHEt) OH 2 H 4-(CONHOH 2 -2-PYRR)PHENYL OH 2 H 4-(OCH 2 C0NH 2 )PHENYL OH 2 R3 OH 4-(OCH 2 C0 2 H)PHENYL CH 2 H

69. A compound selected from: R X NRR' CH2 NHn-C12H25 CH2 HN -_,OMe CH2 HN CH2 HN- 4 O CH2 HN-G0 WO 00/27822 WO 0027822PCT/US99/26 105 CH2 N'D CH2 CH2 N CH2 HNO/ CH2 HN H~( CH2 H~~ o N No oH HN-( oH HN-j o HN- "--Me 0HN WeN oN o HN--o o HNNCN\ S ~HNYH1i SN 0S WO 00/27822 WO 0027822PCTIUS99/26 105 S H-C CH2 NHiso-Pr CH2 NHcyc-PR CH2 NHn-Hex CH2 HN-C N CH2 No CH2 N NWe CH2 N 0 CH2 HN OMe CH2 HN OH N CH2 HN N CH2 HN /02 CH2 HN /\N HN/ N- CH2 NH HN 0H2 I O No~ 0 HN- 179 A compound selected from: R' R N X NRR' CH2 H N O (2HCI) CH2 No CH2 N NMe elH2 HN--j CH2 HN-( CH2 HN' CH2 HN0 n r r

71. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents a group of formula in which p represents 0,1 or 2; R, represents H; R, represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted by one or more of the following: a) halo, WO 00/27822 PCT/US99/26105 180 b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a C, 6 alkoxy group optionally substituted by one or more of the following: hydroxy, COOH, an amino group of formula NRhRj, or an amide of the formula CONRhR,, wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo d) optionally substituted phenoxy, e) hydroxy, f) a group of formula CORa or SO 2 R wherein Ra is hydroxy, (C,-C 6 )alkyl, 6 )alkoxy or Ra represents a group of formula NRbRC; where Rb and Rc independently represent hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, phenyl(Co-C 6 )alkyl or heterocyclyl-(C-C 6 )allyl (heterocyclyl tetrahydrofuranyl, furanyl, 1,3-benzodioxole, pyridinyl, and thiophenyl) wherein the alkyl group, the cycloalkyl group, phenyl or heterocyclyl- (Co-C, 6 )alkyl are optionally substituted by one or more of the following: hydroxy, (C-C,)-hyrdroxy, halo, nitro, (C 1 -C 6 )alkyl, (C 1 -C 6 )alkoxy, -O-(C-C6)alkyl-hydroxy a C3-12 cycloalkyl group or an amino group of formula NRRJ; where Rh and Rj independently represent hydrogen, (C 1 -C,2)alkyl, (C 3 C 6 )cycloalkyl, (C 3 -C, 6 )heterocycloalkyl-(Co-C, 6 )alkyl (heterocycloalkyl tetrazole, pyridine, piperidine, pyrazine, imidazole, triazole, morpoline, and piperazine), (C 1 -C 6 )alkenyl, (C-C, 6 )alkynyl, (C 3 -C, 6 )cycloalkenyl-(Co- C 6 )alkyl, hydroxy(C -C 6 )alkyl, amino(C,-C 6 )alkyl, (C,-C,)alkoxy(C,- C)alkyl, mono- or di-(C,-C)alkylamino(C 1 -C, 6 )alkyl, morpholinyl-(C,- C 6 )alkyl, pyrrolidinyl-(Cl-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of halo, hydroxy, nitro, amino, mono- or di-(C 1 C,)alkylamino, 6 )alkyl and (C 1 -C, 6 )alkoxy; or Rh and Rj together with the nitrogen atom to which they are attached represent a four, five, six or seven membered heterocyclic ring which optionally contains one or more WO 00/27822 PCT/US99/26105 181 additional hetero atoms selected from O, S and N and is optionally substituted by a C1-6 alkyl group or a heterocycle, or Rb and R, are taken together with the nitrogen atom to which they are attached to form an optionally substituted 6- or 7-membered ring where said ring optionally contains one or more additional heteroatoms selected from the group consisting of O, N and S, and said ring is optionally substituted by (C,-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl, phenyl(C 2 -C 6 )alkenyl, where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of Br, Cl, F, I, hydroxy, nitro, amino, mono- or di-(C,-C,)alkylamino, (CI-C,)alkyl and (C,-C 6 )alkoxy; g) a group of formula NRdRe in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, S(0) 2 -phenyl, phenyl, a heterocycloalkyl-(C,-C,)alkyl, wherein the heterocycloalkyl is a four, five, six or seven menbered heterocyclic ring which has one or more heteroatoms selected from the group consisting of O, S and N, or Rd and R, are each, independently,a group of formula CORf wherein Rf represents hydrogen, NRbR, (C,-C 6 )alkoxy, amino-(C,- C 6 )alkoxy-(Ci-C 6 )alkoxy, mono-(C 1 -C 6 )alkyl-amino-(C,-C 6 )alkoxy-(C,-C 6 )alkoxy, N,N-di-(C,-C 6 )alkyl-amino-(C-C 6 )alkoxy-(C,-C 6 )alkoxy, a C1-12 alkyl group, a C3- 12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkoxy, the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro, (Ci-C 6 )alkyl, (C,-C 6 )alkoxy, di-(C 1 -C,)alkyl-amino-(C-C 6 )alkoxy, pyrrolidine which is optionally substituted with a (C-C,)alkyl, or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and mis 1, 2, 3, 4 or i) nitro, j) optionally substituted phenyl C1-6 alkyl, WO 00/27822 PCT/US99/26105 182 k) optionally substituted phenyl C1-6 alkoxy 1) cyano, m) a C 3 6 alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, r) a C1-6 alkythio group s) phenyl t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, u) CHO, v) dihydroxyborane w) tetrazolyl; and R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C,. 6 alkoxy group optionally substituted by one or more of the following: hydroxy, a C- 6 alkoxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group, (C 3 -C 6 )heterocycloalkyl-(Co-C 6 )alkyl (heterocycloalkyl pyridine, morpoline, piperazine, and N-methylpiperazine) or wherein Rh and Rj together with 183 the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a Cl-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2- 4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following: a C1-6 alkyl group, a C1-6 alkoxy group or halo; with the provisos that 1) when R1, R3, R4, R5 and R6 each represent hydrogen and X 20 represents SO,, then R 2 does not represent phenyl and 2) when X represents S and R1, R3, R4, R5 and R6 each represent hydrogen, then R 2 does not represent 2,4-dichlorophenyl.

72. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents oxygen; R, represents H; R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted by one or more of the following: 30 a) halo, WO 00/27822 PCT/US99/26105 184 b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a C, -alkoxy group optionally substituted by one or more of the following: hydroxy, COOH, an amino group of formula NRhRj, or an amide of the formula CONRhRI, wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo d) optionally substituted phenoxy, e) hydroxy, f) a group of formula CORa or SO 2 Ra wherein Ra is hydroxy, (C 1 -C 6 )alkyl, (C,.C 6 )alkoxy or Ra represents a group of formula NRbR,; where Rb and Re independently represent hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, phenyl(Co-C 6 )alkyl or heterocyclyl-(Co-C 6 )alkyl (heterocyclyl tetrahydrofuranyl, furanyl, 1,3-benzodioxole, pyridinyl, and thiophenyl) wherein the alkyl group, the cycloalkyl group, phenyl or heterocyclyl- (Co-C 6 )alkyl are optionally substituted by one or more of the following: hydroxy, (Cl-C6)-hyrdroxy, halo, nitro, (C,-C 6 )alkyl, (Cl-C 6 )alkoxy, 6 )alkyl-hydroxy a C3-12 cycloalkyl group or an amino group of formula NRhR); where Rh and Rj independently represent hydrogen, (C,-C 12 )alkyl, (C 3 C 6 )cycloalkyl, (C 3 -C 6 )heterocycloalkyl-(Co-C 6 )alkyl (heterocycloalcyl tetrazole, pyridine, piperidine, pyrazine, imidazole, triazole, morpoline, and piperazine), (C,-C 6 )alkenyl, (C -C 6 )alkynyl, (C 3 -C 6 )cycloalkenyl-(C,- C 6 )alkyl, hydroxy(C -C 6 )alkyl, amino(Cl-C 6 )alkyl, (C-C 6 )alkoxy(C,- C 6 )alkyl, mono- or di-(C 1 -C 6 )alkylamino(C-C 6 )alkyl, morpholinyl-(C,- C 6 )alkyl, pyrrolidinyl-(C,-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of halo, hydroxy, nitro, amino, mono- or di-(C,- C 6 )alkylamino, (C 1 -C 6 )alkyl and (C,-C,)alkoxy; or Rh and Rj together with the nitrogen atom to which they are attached represent a four, five, six or seven membered heterocyclic ring which optionally contains one or more WO 00/27822 PCT/US99/26105 185 additional hetero atoms selected from 0, S and N and is optionally substituted by a C1-6 alkyl group or a heterocycle, or Rb and R, are taken together with the nitrogen atom to which they are attached to form an optionally substituted 6- or 7-membered ring where said ring optionally contains one or more additional heteroatoms selected from the group consisting of O, N and S, and said ring is optionally substituted by (C 1 -C 6 )alkyl, pyridinyl, phenyl(C 0 -C 6 )alkyl, phenyl(C 2 -C 6 )alkenyl, where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of Br, Cl, F, I, hydroxy, nitro, amino, mono- or di-(C,-C 6 )alkylamino, (C,-C 6 )alkyl and (C,-C 6 )alkoxy; g) a group of formula NRdRe in which Rd and Re are independently selected. from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, S(O) 2 -phenyl, phenyl, a heterocycloalkyl-(C,-C 6 )alkyl, wherein the heterocycloalkyl is a four, five, six or seven menbered heterocyclic ring which has one or more heteroatoms selected from the group consisting of O, S and N, or Rd and R, are each, independently,a group of formula CORf wherein Rf represents hydrogen, NRbR, (C 1 -C 6 )alkoxy, amino-(C,- C 6 )alkoxy-(C,-C 6 )alkoxy, mono-(C -C 6 )alkyl-amino-(C -C 6 )alkoxy-(C 1 -C 6 )alkoxy, N,N-di-(C,-C 6 )alkyl-amino-(C,-C 6 )alkoxy-(C,-C 6 )alkoxy, a C1-12 alkyl group, a C3- 12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkoxy, the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro, (Cl-C 6 )alkyl, (CI-C 6 )alkoxy, di-(C 1 -C 6 )alkyl-amino-(C,-C 6 )alkoxy, pyrrolidine which is optionally substituted with a (C-C 6 )alkyl, or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or i) nitro, j) optionally substituted phenyl C1-6 alkyl, WO 00/27822 PCT/US99/26105 186 k) optionally substituted phenyl C1-6 alkoxy 1) cyano, m) a C3.alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, r) a C1-6 alkythio group s) phenyl t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, u) CHO, v) dihydroxyborane w) tetrazolyl; and R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C,. 6 alkoxy group optionally substituted by one or more of the following: hydroxy, a C.6alkoxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C 1-6 alkyl group, (C 3 -C 6 )heterocycloalkyl-(Co-C 6 )alkyl 187 (heterocycloalkyl pyridine, morpoline, piperazine, and N- methylpiperazine) or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group a phenyl C1-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the S 20 following: a C1-6 alkyl group, a C1-6 alkoxy group or halo. with the proviso that when RI, R3, R4, R5 and R6 each represent hydrogen, then R 2 does not represent phenyl, 2,4-dimethylphenyl or 2,4-dichlorophenyl.

73. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents a group of formula NR,; R, represents H; R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is S 30 optionally substituted by one or more of the following: a) halo, WO 00/27822 PCT/IUS99/26105 188 b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a CI- 6 alkoxy group optionally substituted by one or more of the following: hydroxy, COOH, an amino group of formula NRhRj, or an amide of the formula CONRI,R, wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo d) optionally substituted phenoxy, e) hydroxy, f) a group of formula CORa or SO 2 Ra wherein Ra is hydroxy, (C 1 -C 6 )alkyl, (C,.C 6 )alkoxy or Ra represents a group of formula NRbRC; where Rb and Rc independently represent hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, phenyl(Co-C 6 )alkyl or heterocyclyl-(Co-C 6 )alkyl (heterocyclyl tetrahydrofuranyl, furanyl, 1,3-benzodioxole, pyridinyl, and thiophenyl) wherein the alkyl group, the cycloalkyl group, phenyl or heterocyclyl- (Co-C 6 )alkyl are optionally substituted by one or more of the following: hydroxy, (C-C 6 )-hyrdroxy, halo, nitro, (C 1 -C 6 )alkyl, (C,-C 6 )alkoxy, -O-(C 1 -C 6 )alkyl-hydroxy a C3-12 cycloalkyl group or an amino group of formula NRhJ where Rh and Rj independently represent hydrogen, (C 1 -C 12 )alkyl, (C 3 C 6 )cycloalkyl, (C 3 -C,)heterocycloalkyl-(Co-C,)alkyl (heterocycloalkyl tetrazole, pyridine, piperidine, pyrazine, imidazole, triazole, morpoline, and piperazine), (C-C 6 )alkenyl, (C 1 -C 6 )alkynyl, (C 3 -C 6 )cycloalkenyl-(Co- C)alkyl, hydroxy(C -C 6 )alkyl, amino(C,-C 6 )alkyl, (C 1 -C 6 )alkoxy(C,- C6)alkyl, mono- or di-(C 1 -C 6 )alkylamino(C-C 6 )alkyl, morpholinyl-(C- C 6 )alkyl, pyrrolidinyl-(C-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of halo, hydroxy, nitro, amino, mono- or di-(C,- C)alkylamino, (C 1 -C 6 )alkyl and (C,-C 6 )alkoxy; or Rh and Rj together with the nitrogen atom to which they are attached represent a four, five, six or seven membered heterocyclic ring which optionally contains one or more WO 00/27822 PCT/US99/26105 189 additional hetero atoms selected from O, S and N and is optionally substituted by a C1-6 alkyl group or a heterocycle, or Rb and Re are taken together with the nitrogen atom to which they are attached to form an optionally substituted 6- or 7-membered ring where said ring optionally contains one or more additional heteroatoms selected from the group consisting of O, N and S, and said ring is optionally substituted by (C 1 -C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl, phenyl(C 2 -C 6 )alkenyl, where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of Br, Cl, F, I, hydroxy, nitro, amino, mono- or di-(C,-C 6 )alkylamino, (C,-C 6 )alkyl and (C 1 -C 6 )alkoxy; g) a group of formula NRdRe in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, S(O) 2 -phenyl, phenyl, a heterocycloalkyl-(C-C 6 )alkyl, wherein the heterocycloalkyl is a four, five, six or seven menbered heterocyclic ring which has one or more heteroatoms selected from the group consisting of O, S and N, or Rd and R, are each, independently,a group of formula CORf wherein Rf represents hydrogen, NRRc, (Ci-C 6 )alkoxy, amino-(C 1 C 6 )alkoxy-(C,-C 6 )alkoxy, mono-(C 1 -C 6 )alkyl-amino-(C -C 6 )alkoxy-(C -C 6 )alkoxy, N,N-di-(C-C 6 )alkyl-amino-(C-C 6 )alkoxy-(C,-C 6 )alkoxy, a C1-12 alkyl group, a C3- 12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkoxy, the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro, (C,-C 6 )alkyl, (C 1 -C 6 )alkoxy, di-(C,-C6)alkyl-amino-(C,-C 6 )alkoxy, pyrrolidine which is optionally substituted with a (C 1 -C 6 )alkyl, or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or i) nitro, j) optionally substituted phenyl C1-6 alkyl, WO 00/27822 PCT/US99/26105 190 k) optionally substituted phenyl C1-6 alkoxy 1) cyano, m) a C3. 6 alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, r) a C1-6 alkythio group s) phenyl t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, u) CHO, v) dihydroxyborane w) tetrazolyl; and R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C, 6 alkoxy group optionally substituted by one or more of the following: hydroxy, a C-.alkoxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1 -6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group, (C 3 -C,)heterocycloalkyl-(Co-C,)alkyl 191 (heterocycloalkyl pyridine, morpoline, piperazine, and N- methylpiperazine) or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group a phenyl C1-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is-1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the 20 following: a Cl-6 alkyl group, a C1-6 alkoxy group or halo.

74. Compounds of formula I as defined in Claim 1 and pharmaceutically thereof in which: X represents a group of formula -C=NOR7 in which R7 represents H or a C1-4 alkyl group; R, represents H; R, represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted by one or more of the following: a) halo, r r r r r acceptable salts WO 00/27822 PCT/US99/26105 192 b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a C, 6 alkoxy group optionally substituted by one or more of the following: hydroxy, COOH, an amino group of formula NRhRj, or an amide of the formula CONR,R, wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo d) optionally substituted phenoxy, e) hydroxy, f) a group of formula CORa or SO 2 Ra wherein Ra is hydroxy, (C 1 -C,)alkyl, (C,.C,)alkoxy or Ra represents a group of formula NRbRC; where Rb and Rc independently represent hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, phenyl(Co-C 6 )alkyl or heterocyclyl-(C-C 6 )alkyl (heterocyclyl tetrahydrofuranyl, furanyl, 1,3-benzodioxole, pyridinyl, and thiophenyl) wherein the alkyl group, the cycloalkyl group, phenyl or heterocyclyl- (Co-C6)alkyl are optionally substituted by one or more of the following: hydroxy, (C,-C)-hyrdroxy, halo, nitro, (C,-C 6 )alkyl, (C-C 6 )alkoxy, -O-(C 1 -C)alkyl-hydroxy a C3-12 cycloalkyl group or an amino group of formula NRRj; where Rh and Rj independently represent hydrogen, (C,-C,,)alkyl, (C 3 C 6 )cycloalkyl, (C 3 -C 6 )heterocycloalkyl-(Co-C 6 )alkyl (heterocycloalkyl tetrazole, pyridine, piperidine, pyrazine, imidazole, triazole, morpoline, and piperazine), (C-C 6 )alkenyl, (C,-C 6 )alkynyl, (C 3 -C 6 )cycloalkenyl-(Co- C 6 )alkyl, hydroxy(C 1 -C 6 )alkyl, amino(Cl-C 6 )alkyl, (C,-C 6 )alkoxy(C- C 6 )alkyl, mono- or di-(Cl-C 6 )alkylamino(C 1 -C 6 )alkyl, morpholinyl-(C- C 6 )alkyl, pyrrolidinyl-(Cl-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of halo, hydroxy, nitro, amino, mono- or di-(C,- Cj)alkylamino, (C,-C 6 )alkyl and (C,-C)alkoxy; or Rh and Rj together with the nitrogen atom to which they are attached represent a four, five, six or seven membered heterocyclic ring which optionally contains one or more WO 00/27822 PCT/US99/26105 193 additional hetero atoms selected from O, S and N and is optionally substituted by a C1 -6 alkyl group or a heterocycle, or Rb and R, are taken together with the nitrogen atom to which they are attached to form an optionally substituted 6- or 7-membered ring where said ring optionally contains one or more additional heteroatoms selected from the group consisting of O, N and S, and said ring is optionally substituted by (Cl-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl, phenyl(C 2 -C 6 )alkenyl, where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of Br, Cl, F, I, hydroxy, nitro, amino, mono- or di-(Ci-C 6 )alkylamino, (Cl-C 6 )alkyl and (C,-C 6 )alkoxy; g) a group of formula NRdRe in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, S(O) 2 -phenyl, phenyl, a heterocycloalkyl-(C-C 6 )alkyl, wherein the heterocycloalkyl is a four, five, six or seven menbered heterocyclic ring which has one or more heteroatoms selected from the group consisting of 0, S and N, or Rd and R, are each, independently,a group of formula CORf wherein Rf represents hydrogen, NRbRc, (C,-C 6 )alkoxy, amino-(C,- C 6 )alkoxy-(C 1 -C 6 )alkoxy, mono-(Cl-C 6 )alkyl-amino-(Ci-C 6 )alkoxy-(C,-C 6 )alkoxy, N,N-di-(C,-C 6 )alkyl-amino-(C-C 6 )alkoxy-(C,-C 6 )alkoxy, a C1-12 alkyl group, a C3- 12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkoxy, the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro, (C 1 -C 6 )alkyl, (C-C 6 )alkoxy, di-(Ci-C 6 )alkyl-amino-(C,-C 6 )alkoxy, pyrrolidine which is optionally substituted with a (C-C,)alkyl, or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or i) nitro, j) optionally substituted phenyl C1-6 alkyl, WO 00/27822 PCT/US99/26105 194 k) optionally substituted phenyl C1-6 alkoxy 1) cyano, m) a C3.6alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, r) a C1-6 alkythio group s) phenyl t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, u) CHO, v) dihydroxyborane w) tetrazolyl; and R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C 1 alkoxy group optionally substituted by one or more of the following: hydroxy, a C,.6alkoxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C 1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group, (C 3 -C 6 )heterocycloalkyl-(Co-C 6 )alkyl 195 (heterocycloalkyl pyridine, morpoline, piperazine, and N- methylpiperazine) or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from 0, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group a phenyl C1-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the 20 following: a Cl-6 alkyl group, a C1-6 alkoxy group or halo; with the proviso that when R1, R3, R4, R5 and R6 each represent hydrogen, and X represents C=NOH then R2 does not represent 4-methylphenyl or 3,4-dimethoxyphenyl.

75. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents substituted methylene or a carbonyl group; R, represents H; e R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is 30 optionally substituted by one or more of the following: a) halo, *o WO 00/27822 PCT/US99/26105 196 b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a alkoxy group optionally substituted by one or more of the following: hydroxy, COOH, an amino group of formula NRhRj, or an amide of the formula CONRhRj, wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo d) optionally substituted phenoxy, e) hydroxy, f) a group of formula CORa or SO,Ra wherein Ra is hydroxy, (CI-C 6 )alkyl, (C,.C 6 )alkoxy or Ra represents a group of formula NRbRC,; where Rb and Rc independently represent hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, phenyl(Co-C 6 )alkyl or heterocyclyl-(Co-C 6 )alkyl (heterocyclyl tetrahydrofuranyl, furanyl, 1,3-benzodioxole, pyridinyl, and thiophenyl) wherein the alkyl group, the cycloalkyl group, phenyl or heterocyclyl- (Co-C 6 )alkyl are optionally substituted by one or more of the following: hydroxy, (C 1 -C 6 )-hyrdroxy, halo, nitro, (C,-C 6 )alkyl, (C-C 6 )alkoxy, 6 )alkyl-hydroxy a C3-12 cycloalkyl group or an amino group of formula NRRJ; where Rh and Rj independently represent hydrogen, (C,-C 12 )alkyl, (C 3 C 6 )cycloalkyl, (C 3 -C 6 )heterocycloalkyl-(Co-C 6 )alkyl (heterocycloalkyl= tetrazole, pyridine, piperidine, pyrazine, imidazole, triazole, morpoline, and piperazine), (C,-C 6 )alkenyl, (C,-C 6 )alkynyl, (C 3 -C 6 )cycloalkenyl-(Co- C 6 )alkyl, hydroxy(C,-C 6 )alkyl, amino(C,-C 6 )alkyl, (C 1 -C)alkoxy(C- C 6 )alkyl, mono- or di-(C 1 -C 6 )alkylamino(C,-C 6 )alkyl, morpholinyl-(C,- C 6 )alkyl, pyrrolidinyl-(C,-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of halo, hydroxy, nitro, amino, mono- or di-(C,- C 6 )alkylamino, (C,-C 6 )alkyl and (C,-C 6 )alkoxy; or Rh and Rj together with the nitrogen atom to which they are attached represent a four, five, six or seven membered heterocyclic ring which optionally contains one or more WO 00/27822 PCT/US99/26105 197 additional hetero atoms selected from O, S and N and is optionally substituted by a C1-6 alkyl group or a heterocycle, or Rb and R, are taken together with the nitrogen atom to which they are attached to form an optionally substituted 6- or 7-membered ring where said ring optionally contains one or more additional heteroatoms selected from the group consisting of O, N and S, and said ring is optionally substituted by (C,-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl, phenyl(C 2 -C 6 )alkenyl, where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of Br, Cl, F, I, hydroxy, nitro, amino, mono- or di-(Ci-C 6 )alkylamino, (Ci-C 6 )alkyl and (C 1 -C 6 )alkoxy; g) a group of formula NRdRe in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, S(0) 2 -phenyl, phenyl, a heterocycloalkyl-(Ci-C 6 )alkyl, wherein the heterocycloalkyl is a four, five, six or seven menbered heterocyclic ring which has one or more heteroatoms selected from the group consisting of O, S and N, or Rd and R, are each, independently,a group of formula CORf wherein Rf represents hydrogen, NRbRc, (C 1 -C 6 )alkoxy, amino-(C,- C6)alkoxy-(C,-C 6 )alkoxy, mono-(C,-C 6 )alkyl-amino-(C,-C 6 )alkoxy-(C,-C 6 )alkoxy, N,N-di-(C,-C 6 )alkyl-amino-(C,-C 6 )alkoxy-(C 1 -C 6 )alkoxy, a C1-12 alkyl group, a C3- 12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkoxy, the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro, (C,-C 6 )alkyl, (C 1 -C 6 )alkoxy, di-(C 1 -C 6 )alkyl-amino-(Ci-C,)alkoxy, pyrrolidine which is optionally substituted with a (C,-C 6 )alkyl, or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or i) nitro, j) optionally substituted phenyl C1-6 alkyl, WO 00/27822 PCT/US99/26105 198 k) optionally substituted phenyl C1-6 alkoxy 1) cyano, m) a C3 6 alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, r) a C1-6 alkythio group s) phenyl t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, u) CHO, v) dihydroxyborane w) tetrazolyl; and R3, R4, R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C, 6 alkoxy group optionally substituted by one or more of the following: hydroxy, a C,. 6 alkoxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C 1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group, (C 3 -C 6 )heterocycloalkyl-(Co-C 6 )alkyl b 05-01-2001 US 009926105

2079.1002001 -199- (heterocycloalkyl pyridine, morpholine, piperazine, and N- methylpiperazine) or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from 0, S or N and is optionally substituted by a C1-. alkyl group, h) a group of formula NRdR, in which Rd and R, are independently selected from hydrogen, a C 1 2 alkyl group, a C3. 1 2 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a Cz. 12 alkyl group, a C 3 -12 cycloalkyl group, a phenyl Cj. alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRR wherein Rh and Rj are as defined above i) a group of formula O(CH 2 )A in which m is 2, 3, 4 or 5 and R, represents hydroxy or a group of formula NRR in which Rd and R, are as defined above; or R, represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or j) nitro, k) optionally substituted phenyl CI. 6 alkyl, 1) optionally substituted phenyl alkoxy m) cyano or o) a C 2 -4 alkenyl group or a C2 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following: a CI- alkyl group, a C,6 alkoxy group or halo; with the provisos that 1) when X represents carbonyl or a substituted methylene, R, is hydrogen and R 3 R 4 R, and R each represent hydrogen, then R 2 is not 2-pyrrolyl, pyridyl, 2-thienyl, 3-thienyl, phenyl or phenyl substituted by C,. 2 alkyl, a halogen atom, a lower alkoxy group, a hydroxyl group or an amino group; 2) when X represents methylene, R, is hydrogen and two of R 3 R 4 R and R, independently represent hydrogen, halogen having an atomic weight of about 19 to 36, C 14 alkyl, C,4 alkoxy or trifluoromethyl and the other two represent hydrogen, then R, is not thienyl, furyl, AMENDED SHEET 200 pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having two or less substituents wherein the substituents are halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl; and 3) when X represents a carbonyl group, R2 represents phenyl, 4- chlorophenyl or 4-methoxyphenyl, then R3, R4, R5 and R6 are not trifluoromethyl; and 4) when X represents a carbonyl group, R2 represents phenyl, R3 represents bromo, R4 represents hydroxy and R5 represents methoxy, then R6 is not hydrogen and when X represents carbonyl and R3, R4, R5 and R6 represent hydrogen, then R6 is not aryl. 76. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents a group of formula (CH2)n in which n is 1, 2 or 3 20 R, represents H; R represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted by one or more of the following: a) halo, b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a alkoxy group optionally substituted by one or more of the following: hydroxy, COOH, an amino group of formula NRhRj, or an amide of the formula CONRIRj, wherein Rh and Rj are as defined below provided that these groups are 30 not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo WO 00/27822 PCT/US99/26105 201 d) optionally substituted phenoxy, e) hydroxy, f) a group of formula CORa or SO.Ra wherein Ra is hydroxy, (C 1 -C 6 )alkyl, (C,.C,)alkoxy or Ra represents a group of formula NR,,R,; where Rb and Rc independently represent hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, phenyl(Co-C 6 )alkyl or heterocyclyl-(Co-C 6 )alkyl (heterocyclyl tetrahydrofuranyl, furanyl, 1,3-benzodioxole, pyridinyl, and thiophenyl) wherein the alkyl group, the cycloalkyl group, phenyl or heterocyclyl- (Co-C 6 )alkyl are optionally substituted by one or more of the following: hydroxy, (C,-C 6 )-hyrdroxy, halo, nitro, (C,-C 6 )alkyl, (C-C 6 )alkoxy, -O-(C,-C)alkyl-hydroxy a C3-12 cycloalkyl group or an amino group of formula NRhRj, where Rh and Rj independently represent hydrogen, (C 1 -C 12 )alkyl, (C 3 C 6 )cycloalkyl, (C 3 -C 6 )heterocycloalkyl-(Co-C 6 )alkyl (heterocycloalkyl= tetrazole, pyridine, piperidine, pyrazine, imidazole, triazole, morpoline, and piperazine), (C 1 -C 6 )alkenyl, (C,-C 6 )alkynyl, (C 3 -C 6 )cycloalkenyl-(Co- C)alkyl, hydroxy(C,-C,)alkyl, amino(CI-C 6 )alkyl, (C-C 6 )alkoxy(Cl- C 6 )alkyl, mono- or di-(C,-C 6 )alkylamino(C,-C 6 )alkyl, morpholinyl-(C,- C 6 )alkyl, pyrrolidinyl-(C,-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of halo, hydroxy, nitro, amino, mono- or di-(C,- C 6 )alkylamino, (C 1 -C 6 )alkyl and (C,-C 6 )alkoxy; or Rh and Rj together with the nitrogen atom to which they are attached represent a four, five, six or seven membered heterocyclic ring which optionally contains one or more additional hetero atoms selected from 0, S and N and is optionally substituted by a C1-6 alkyl group or a heterocycle, or Rb and R, are taken together with the nitrogen atom to which they are attached to form an optionally substituted 6- or 7-membered ring where said ring optionally contains one or more additional heteroatoms selected from the group consisting of O, N and S, and said ring is optionally substituted by (CI-C,)alkyl, pyridinyl, phenyl(Co-C 6 )alkyl, phenyl(C 2 -C 6 )alkenyl, where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of WO 00/27822 PCT/US99/26105 202 Br, C1, F, I, hydroxy, nitro, amino, mono- or di-(C,-C 6 )alkylamino, (C,-C 6 )alkyl and (C-C 6 )alkoxy; g) a group of formula NRdRe in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, S(O) 2 -phenyl, phenyl, a heterocycloalkyl-(C-C 6 )alkyl, wherein the heterocycloalkyl is a four, five, six or seven menbered heterocyclic ring which has one or more heteroatoms selected from the group consisting of O, S and N, or Rd and R, are each, independently,a group of formula CORf wherein Rfrepresents hydrogen, NRbRc, (C,-C 6 )alkoxy, amino-(C- C 6 )alkoxy-(C -C 6 )alkoxy, mono-(C 1 -C 6 )alkyl-amino-(C,-C 6 )alkoxy-(C,-C 6 )alkoxy, N,N-di-(C,-C 6 )alkyl-amino-(C 1 -C 6 )alkoxy-(C,-C 6 )alkoxy, a C1-12 alkyl group, a C3- 12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkoxy, the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro, (C,-C 6 )alkyl, (C 1 -C 6 )alkoxy, di-(C-C 6 )alkyl-amino-(C,-C 6 )alkoxy, pyrrolidine which is optionally substituted with a (C-C 6 )alkyl, or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or i) nitro, j) optionally substituted phenyl C1-6 alkyl, k) optionally substituted phenyl C1-6 alkoxy 1) cyano, m) a C 36 alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, WO 00/27822 PCT/US99/26105 203 r) a C1 -6 alkythio group s) phenyl t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, u) CHO, v) dihydroxyborane w) tetrazolyl; and R3, R4 R5 and R6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C,. 6 alkoxy group optionally substituted by one or more of the following: hydroxy, a C,.alkoxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C 1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C1-6 alkyl group, (C 3 -C 6 )heterocycloalkyl-(C 0 -C 6 )alkyl (heterocycloalkyl pyridine, morpoline, piperazine, and N- methylpiperazine) or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 204 alkyl group a C3-12 cycloalkyl group a phenyl C1-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl Cl-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following: a C1-6 alkyl group, a C1-6 alkoxy group or halo. with the proviso that when X is a group of formula (CH 2 in which n is 1, 2 or 3, R, is hydrogen and two of R3, R4, R5 and R6 independently represent hydrogen, halogen having an atomic weight of about 19 to 36, C1-4 alkyl, C1-4 alkoxy or trifluoromethyl and the other two represent hydrogen, then R2 is not thienyl, furyl, pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having two Sor less substituents wherein the substituents are halogen having an atomic S 20 weight of about 19 to 36, Cl-4 alkyl, C1-4 alkoxy or trifluoromethyl, and with the proviso that S• when n is 2 and R3, R4, R5 and R6 each represent hydrogen or methoxy, then R2 does not represent 3-carboxypyrid-2-yl, 3-methoxycarbonylpyrid-2- yl or 2-carboxyphenyl 77. Compounds of formula I as defined in Claim 1 and pharmaceutically acceptable salts thereof in which: X represents a) substituted methylene b) carbonyl, c) oxygen, d) a group of formula -C=NOR7 in which R7 represents H or a C1-4 alkyl group, e) a 30 group of formula NRg in which Rg represents H, an optionally substituted Qeoe WO 00/27822 PCT/US99/26105 205 C1-4 alkyl group or optionally substituted phenyl, f) a group of formula (CH 2 in which n is 1, 2 or 3, or g) a group of formula S(0)p in which p is 0, 1 or 2; R, represents H; R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted by one or more of the following: a) halo, b) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, c) a C, 6 alkoxy group optionally substituted by one or more of the following: hydroxy, COOH, an amino group of formula NRhRj, or an amide of the formula CONRhR, wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo d) optionally substituted phenoxy, e) hydroxy, f) a group of formula CORa or S0 2 R wherein Ra is hydroxy, (C 1 -C 6 )alkyl, (C, 1 C 6 )alkoxy or Ra represents a group of formula NRbRC; where Rb and Rc independently represent hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, phenyl(Co-C, 6 )alkyl or heterocyclyl-(Co-C, 6 )alkyl (heterocyclyl tetrahydrofuranyl, furanyl, 1,3-benzodioxole, pyridinyl, and thiophenyl) wherein the alkyl group, the cycloalkyl group, phenyl or heterocyclyl- (C-C, 6 )alkyl are optionally substituted by one or more of the following: hydroxy, (C-C 6 )-hyrdroxy, halo, nitro, (CI-C, 6 )alkyl, (C,-C)alkoxy, -O-(Cl-C, 6 )alkyl-hydroxy a C3-12 cycloalkyl group or an amino group of formula NRR; where Rh and Rj independently represent hydrogen, (C,-C,,)alkyl, (C 3 C, 6 )cycloalkyl, (C 3 -C, 6 )heterocycloalkyl-(Co-C 6 )alkyl (heterocycloalkyl tetrazole, pyridine, piperidine, pyrazine, imidazole, triazole, morpoline, and piperazine), (C 1 -C, 6 )alkenyl, (C 1 -C, 6 )alkynyl, (C3-C, 6 )cycloalkenyl-(Co- C 6 )alkyl, hydroxy(C,-Cj)alkyl, amino(C,-C 6 )alkyl, (CI-C 6 )alkoxy(C,- WO 00/27822 PCT/US99/26105 206 C 6 )alkyl, mono- or di-(C,-C 6 )alkylamino(C-C 6 )alkyl, morpholinyl-(C,- C 6 )alkyl, pyrrolidinyl-(Cl-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of halo, hydroxy, nitro, amino, mono- or di-(C,- C 6 )alkylamino, (C,-C 6 )alkyl and (C,-C 6 )alkoxy; or Rh and Rj together with the nitrogen atom to which they are attached represent a four, five, six or seven membered heterocyclic ring which optionally contains one or more additional hetero atoms selected from 0, S and N and is optionally substituted by a C1-6 alkyl group or a heterocycle, or Rb and R. are taken together with the nitrogen atom to which they are attached to form an optionally substituted 6- or 7-membered ring where said ring optionally contains one or more additional heteroatoms selected from the group consisting of 0, N and S, and said ring is optionally substituted by (C,-C 6 )alkyl, pyridinyl, phenyl(Co-C 6 )alkyl, phenyl(C 2 -C 6 )alkenyl, where the phenyl portion is optionally substituted by one or more moieties selected from the group consisting of Br, Cl, F, I, hydroxy, nitro, amino, mono- or di-(C,-C 6 )alkylamino, (Ci-C 6 )alkyl and (C 1 -C 6 )alkoxy; g) a group of formula NRdRe in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group, a C3-12 cycloalkyl group, S(O) 2 -phenyl, phenyl, a heterocycloalkyl-(C 1 -C 6 )alkyl, wherein the heterocycloalkyl is a four, five, six or seven menbered heterocyclic ring which has one or more heteroatoms selected from the group consisting ofO, S and N, or Rd and R, are each, independently,a group of formula CORf wherein Rfrepresents hydrogen, NRbR, (Ci-C 6 )alkoxy, amino-(C- C 6 )alkoxy-(C,-C 6 )alkoxy, mono-(C,-C 6 )alkyl-amino-(C 1 -C 6 )alkoxy-(C,-C 6 )alkoxy, N,N-di-(C,-C 6 )alkyl-amino-(Ci-C 6 )alkoxy-(C-C 6 )alkoxy, a C1-12 alkyl group, a C3- 12 cycloalkyl group, a phenyl C1-6 alkyl group or phenyl wherein in each case the alkoxy, the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro, (C,-C 6 )alkyl, (C,-C 6 )alkoxy, di-(C,-C 6 )alkyl-amino-(C,-C 6 )alkoxy, pyrrolidine which is optionally substituted WO 00/27822 PCT/US99/26105 207 with a (C,-C 6 )alkyl, or an amino group of formula NRhRj wherein Rh and Rj are as defined above, h) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3,4 or i) nitro, j) optionally substituted phenyl C1-6 alkyl, k) optionally substituted phenyl C1-6 alkoxy 1) cyano, m) a C 3 6 alkenyloxy group, n) a pyridyloxy or pyridylthio group in which the pyridine ring is optionally substituted by one or more of the following trifluoromethyl or nitro, o) hydroxyamidino, p) aminomethyl, q) formamidomethyl, r) a C1-6 alkythio group s) phenyl t) a C2-4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following a C1-6 alkyl group, a C1-6 alkoxy group or halo, u) CHO, v) dihydroxyborane w) tetrazolyl; R3, R4 R and R6 independently represent a) H, b) halo, c) a Cl-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C 1 6 alkoxy group optionally substituted by one or more of the following: hydroxy, a C,-.alkoxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the WO 00/27822 PCT/US99/26105 208 oxygen of the alkoxy group, e) optionally substituted phenoxy, f) hydroxy, g) a group formula CORa in which Ra represents hydroxy, a C1-6 alkoxy group or Ra represents a group of formula NRb Rc in which Rb and Rc independently represent hydrogen, a C1-6 alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C 1 -6 alkyl group, (C 3 -C 6 )heterocycloalkyl-(Co-C)alkyl (heterocycloalkyl pyridine, morpoline, piperazine, and N-methylpiperazine) or wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional hetero atom selected from O, S or N and is optionally substituted by a C1-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a C1-12 alkyl group a C3-12 cycloalkyl group a phenyl C 1-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above i) a group of formula O(CH2)m Rg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl C1-6 alkyl, 1) optionally substituted phenyl C1-6 alkoxy m) cyano or o) a C2- 4 alkenyl group or a C2-4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following: a C1-6 alkyl group, a C1-6 alkoxy group or halo. 209 78. A use of a compound represented by the formula: R 3 R 4 R2 N Formula I N R6 R, and pharmaceutically acceptable salts thereof in which: X represents a) substituted methylene, b) carbonyl, c) oxygen, d) a group of formula -C=NOR 7 in which R 7 represents H or a C 1 4 alkyl group, e) a group of formula NR 8 in which R 8 represents H, an optionally substituted C1- 4 alkyl group or optionally substituted phenyl, f) a group of formula (CH 2 )n in which n is 1, 2 or 3, or g) a group of formula S(O)p in which p is 0, 1 or 2; R 1 represents H; R 2 represents aryl, pyridyl, thienyl, furyl or pyrrolyl each of which is optionally substituted; R 3 R 4 Rs and R 6 independently represent a) H, b) halo, c) a C1-6 alkyl group optionally substituted by one or more of the following: hydroxy, halo or an amino group of formula NRhRj wherein Rh and Rj are as defined below, d) a C1- 6 alkoxy group optionally substituted by one or more of the following: hydroxy, or an amino group of formula NRhRj wherein Rh and Rj are as defined below provided that these groups are not attached to the carbon which is attached to the oxygen of the alkoxy group; or halo, e) optionally substituted phenoxy, f) hydroxy, g) a group of formula CORa in which Ra represents hydroxy, a C 1 6 alkoxy group or Ra represents a group of formula NRbRc in which Rb and Re independently represent hydrogen, a C 1 .e alkyl group or phenyl wherein the alkyl group and phenyl are optionally substituted by one or more of the following: hydroxy or an amino group of formula NRhRj wherein Rh and Rj independently represent hydrogen or a C 1 -6 alkyl group or 20/05 '03 TUE 11:29 [TX/RX NO 9359] 210 wherein Rh and Rj together with the nitrogen atom to which they are attached represent a five, six or seven membered saturated heterocyclic ring which optionally contains an additional heteroatom selected from 0, S or N and is optionally substituted by a CI-6 alkyl group, h) a group of formula NRd Re in which Rd and Re are independently selected from hydrogen, a Ci- 1 2 alkyl group, a C 3 1 2 cycloalkyl group or phenyl or a group of formula CORf wherein Rf represents hydrogen, a Ci-1 2 alkyl group, a C3- 12 cycloalkyl group, a phenyl Ci-6 alkyl group or phenyl wherein in each case the alkyl group, the cycloalkyl group and phenyl are optionally substituted by one or more of the following: halo, hydroxy, nitro or an amino group of formula NRhRj wherein Rh and Rj are as defined above, i) a group of formula O(CH 2 )mRg in which m is 2, 3, 4 or 5 and Rg represents hydroxy or a group of formula NRdRe in which Rd and Re are as defined above; or Rg represents a group of formula CORa wherein Ra is as defined above and m is 1, 2, 3, 4 or 5, j) nitro, k) optionally substituted phenyl CI-6 alkyl, 1) optionally substituted phenyl Ci-6 alkoxy m) cyano or o) a C 2 4 alkenyl group or a C 2 -4 alkynyl group each of which is optionally substituted by phenyl which is optionally substituted by one or more of the following: a Ci. 6 alkyl group, a Ci-6 alkoxy group or halo, with the provisos that 1) when X represents carbonyl or a substituted methylene, R, is hydrogen and R 3 R 4 R 5 and R 6 each represent hydrogen, then R 2 is not pyridyl, phenyl or phenyl substituted by CI- 2 alkyl, a halogen atom, a lower alkoxy group, a hydroxyl group or an amino group; and 2) when X is a group of formula (CH 2 )n in which n is 1, 2 or 3, R 1 is hydrogen and two of R 3 R 4 Rs and R 6 independently represent hydrogen, halogen having an atomic weight of about 19 to 36, CI.4 alkyl, CI. 4 alkoxy or trifluoromethyl and the other two represent hydrogen, then R 2 is not thienyl, fury, pyrrolyl, pyridyl, each of which is unsubstituted, or phenyl having two or less substituents wherein the substituents are halogen having an atomic weight of about 19 to 36, CI- 4 alkyl, Ci-4 alkoxy or trifluoromethyl; to said kinase in the manufacture of a medicament for use in inhibiting the enzyme activity of said kinase. 79. The use as claimed in Claim 78 wherein said protein kinase is a tyrosine kinase. oo 79. The use as claimed in Claim 78 wherein said protein kinase is a tyrosine kinase. *oo *o 211 The use as claimed in Claim 79 wherein said tyrosine kinase is either a receptor tyrosine kinase or a non-receptor tyrosine kinase. 81. The use as claimed in Claim 80 wherein said tyrosine kinase is selected from the group consisting of KDR, fit-1, TIE-2, Lck, Src, fyn and yes. 82. The use as claimed in Claim 78 wherein the activity of said tyrosine kinase affects angiogenesis. 83. The use as claimed in Claim 82 wherein the inhibition of said tyrosine kinase is anti-angiogenic. 84. The use as claimed in Claim 83 wherein said inhibition of said tyrosine kinase inhibits the progression of a disease state selected from the group consisting of cancer, arthritis, atherosclerosis, psoriasis, hemangioma, myocardial angiogenesis, coronary and cerebral collateral vascularization, ischemic limb angiogenesis, corneal disease, rubeosis, neovascular glaucoma, macular degeneration, retinopathy of prematurity, wound healing, ulcers, Helicobacter related diseases, fractures, endometriosis, diabetic retinopathy, cat scratch fever, and thyroid hyperplasia. S 85. The use as claimed in Claim 79 wherein the activity of said tyrosine kinase affects vascular hyperpermeability or the production of edema. 86.The use as claimed in Claim 85 wherein the inhibition of said tyrosine kinase is antiedematous. 87. The use as claimed in Claim 86, wherein the inhibition of said tyrosine kinase inhibits the progression of a disease state selected from the group consisting of bums, trauma, chronic lung i disease, stroke, polyps, cysts, synovitis, psoriasis, chronic and allergic inflammation, macular degeneration, diabetic retinopathy, retinopathy of prematurity, ovarian hyperstimulation syndrome, *go• 212 pulmonary and cerebral edema, keloid, fibrosis, cirrhosis, carpal tunnel syndrome, adult respiratory distress syndrome, ascites and tumor-associated effusions and edema. 88. The use as claimed in Claim 79 wherein the inhibition of said tyrosine kinase has an anti-tumor effect. 89. The use as claimed in Claim 79 wherein the inhibition of said tyrosine activity is associated with anti-fertility or abortifacient effects. A method according to any one of Claims 1 to 12 or 63 to 66, substantially as hereinbefore described with reference to any one of Examples 1 to 209 and Example A. 91. Compounds according to any one of Claims 13 to 58, 60, 61 or 67 to 77 substantially as hereinbefore described with reference to any one of Examples 1 to 209 and Example A. S 92. A composition according to claim 59 substantially as hereinbefore described with reference to any one of Examples 1 to 209 and Example A. 93. The use according to any one of claims 62 or 78 to 89 substantially as hereinbefore described with reference to any one of Examples 1 to 209 and Example A. DATED this 4th day of April, 2003 "BASF AKTIENGESELLSCHAFT WATERMARK PATENT TRADE MARK ATTORNEYS o 290 BURWOOD ROAD HAWTHORN VICTORIA 3122 AUSTRALIA P19457AU00 CJH/EXE/SIG