CN109966283A - Application of the degreasing cinnamon polyphenol extract in preparation prevention and treatment diabetic nephropathy product - Google Patents

Abstract

The invention belongs to the technical field of medicinal natural products, and in particular relates to the application of a defatted cinnamon polyphenol extract extracted from cinnamon bark in preparing a product for preventing and treating diabetic nephropathy. The weight content of total polyphenols in the described defatted cinnamon polyphenol extract is 40% to 80%, and the main active ingredient in the total polyphenols is one or a mixture of cinnamon tannin and cinnamon epicatechin; the The active ingredients can reduce urinary albumin and kidney index in diabetic nephropathy rats, and reduce glycated serum protein, glycated hemoglobin, blood creatinine and blood urea nitrogen in diabetic nephropathy rats; the defatted cinnamon polyphenol extract provided by the present invention can alleviate the large scale of diabetic nephropathy. Kidney damage in mice has the effect of preventing diabetic nephropathy, and is expected to be used for the treatment of diabetic nephropathy and other diseases.

Description

Application of defatted cinnamon polyphenol extract in the preparation of products for the prevention and treatment of diabetic nephropathy

technical field

The invention belongs to the technical field of medicinal natural products, and in particular relates to the application of a defatted cinnamon polyphenol extract extracted from cinnamon bark in the preparation of a product for preventing and treating diabetic nephropathy, in particular to the application of the defatted cinnamon polyphenol extract in the preparation of a product that can alleviate diabetic nephropathy. Use in foods and medicines related to kidney damage and prevention of diabetic nephropathy.

Background technique

In 2013, the incidence of diabetes in my country reached 10.9%, ranking first in the world with 110 million diabetic patients. Diabetic nephropathy is one of the most common serious complications of diabetes and the primary cause of end-stage renal failure. Its treatment mainly adopts comprehensive measures of controlling blood sugar and blood lipids and using diuretics appropriately. However, these therapeutic measures have limited efficacy and there is no targeted treatment. Drugs for diabetic nephropathy appear. The pathogenesis of diabetic nephropathy is very complex and has not yet been fully elucidated, and its clinical efficacy is not ideal. Therefore, the effective prevention and control of diabetic nephropathy is particularly urgent, and it is of great clinical application value to find effective components of traditional Chinese medicine that can improve the renal damage of diabetic nephropathy. In recent years, it has been found that polyphenols can delay the development of diabetic nephropathy.

Cinnamon, [Latin name] Cinnamomum cassia Presl , [alias] Yugui, Mugui, Jungui, is the dry bark of Lauraceae cinnamon, mainly distributed in Guangxi, Guangdong, Yunnan, Fujian in China, of which Guangxi is its most important origin. Cinnamon polyphenols are the main active components of the non-volatile oil part of cinnamon, which have pharmacological effects such as hypoglycemic, anti-tumor, anti-inflammatory, and blood vessel protection [Hu Shaoping. Research progress on the pharmacological effects of cinnamon polyphenols [J]. Yunnan Traditional Chinese Medicine Journal, 2014, 35(5): 79-81.]. Many researchers have reported that cinnamon polyphenols have hypoglycemic effects, and in addition, cinnamon polyphenols have obvious hypolipidemic effects [Liao Zuozhuang. Research progress on the pharmacological effects and mechanisms of cinnamon polyphenols [J]. Youjiang Medicine, 2018, 46(03):359-362. ], cinnamon and its extracts have obvious therapeutic effects on metabolic diseases [Morazade, Husaynzad. Research progress on the mechanism of cinnamon on metabolic syndrome[J] . Iran J Basic Med Sci, 2016, 19:1258-1270.](Mollazadeh H, Hosseinzadeh H. Cinnamon effects on metabolic syndrome: areview based on its mechanisms [J]. Iran J Basic Med Sci, 2016, 19: 1258-1270 .).) Cinnamon, as a traditional Chinese medicinal material for both medicine and food, is mainly used in the clinical treatment of diabetes and its complications, cardiovascular diseases, gastrointestinal diseases, gynecological diseases, etc. in the form of traditional Chinese medicine compounds. More interestingly, studies have shown that cinnamon can reduce blood urea nitrogen levels in diabetic bodies, suggesting the possibility of cinnamon as a primary or adjunct drug for diabetic nephropathy [Odroglu, Sozer, Tarbil, et al. Long-term observation of the toxic effects of bark and olive leaves on streptozotocin in rats[J]. Journal of Pharmacy and Pharmacology, 1999, 51(11):1305-1312.Onderoglu S, SozerS,ErbilKM, etal. The evaluation of long-term effects of cinnamon bark and oliveleaf on toxicity induced by streptozotoc in administration to rats[J]. The Journal of pharmacy and pharmacology, 1999, 51(11): 1305-1312.].

Regarding the effect of cinnamon on reducing diabetic nephropathy, the inventor also retrieved the following documents:

1. [Title] The active components of cinnamon in inhibiting the aggregation of human amylin and the protective mechanism of apelin on diabetic nephropathy [Author] Jiao Lihua [Dissertation] Huazhong University of Science and Technology, 2013. 【Abstract】Studies have found that long-term consumption of cinnamon can alleviate the occurrence of diabetes. The occurrence of amylin misfolding and aggregation in type 2 diabetes is thought to be one of the causes of the disease. This paper investigated the effect of cinnamon on the aggregation toxicity of amylin. The study found that cinnamon water extract can effectively inhibit the aggregation of human amylin, and identified the main component that exerts the inhibitory activity as procyanidin. Proanthocyanidins can affect the secondary structure of human amylin and delay its change from random coil to β-sheet-rich structure. Further research found that proanthocyanidins can not only inhibit the production of human amylin toxic oligomers, but also significantly It significantly reduces cell membrane damage caused by amylin aggregation and cytotoxicity to rat pancreatic cancer cells. Indicate a possible mechanism of cinnamon alleviating the occurrence of diabetes, and suggest the important role of proanthocyanidins in the treatment and prevention of type 2 diabetes.

Document 1 discloses that proanthocyanidins of cinnamon are the main components that inhibit the activity of human insulin amyloid polypeptide, but there is no research on the effect of cinnamon proanthocyanidins on diabetic nephropathy.

2. 【Title】Protective effect of cinnamon-proposed methyl chard ketone on renal damage in diabetic rats with unilateral nephrectomy 【Author】Wu Fan;Gong Chao;Chen Zhiyuan 【Institution】Wuhan University 【Title】Chinese Journal of Experimental Surgery 2009 , 26(11):1515-1518 [Abstract] Objective To observe the effect of cinnamon extract methyl charles polyketone (MHCP) on diabetic kidney damage. Methods The rat model of diabetes was established by unilateral nephrectomy and intraperitoneal injection of streptozotocin. The rats were randomly divided into 3 groups, and then intragastrically administered for 12 weeks. and other indicators, and observed the histomorphological changes of the rat kidneys. Results Methyl Charles Done could partially reduce the expression of vascular endothelial cell production factor (average relative absorbance: 25.3%), insulin receptor substrate-1 (average relative absorbance: 189.5%), and enhance the antioxidant substances in rat kidney cortex. activity. Conclusion Methyl Charles Done can alleviate diabetic kidney damage by increasing the activity of insulin receptor substrate-1, increasing the body's sensitivity to insulin, reducing insulin resistance, controlling blood sugar, reducing oxidative stress and improving endothelial cell function.

3. [Title] Ameliorative effect of the cinnamon oil from Cinnamomum zeylanicum upon early stage diabetic nephropathy. [Author] Mishra A, Bhatti R, Singh A, Singh Ishar MP Medical Factory, 2010, 76(5): 312-417. Planta Med. 2010, 76(5): 412-417 [Abstract] Objective To evaluate the improving effect of cinnamon oil on early diabetic nephropathy. Methods Cinnamon oil was extracted from cinnamon bark by steam distillation. Early stages of diabetic nephropathy were induced by injection of alloxan (150 mg/kg). Animals with successful modeling were divided into three dose groups: 5 mg/kg, 10 mg/kg, and 20 mg/kg of cinnamon oil, and 10 mg/kg of pioglitazone group. Fasting blood glucose, total cholesterol, high-density lipoprotein, urea nitrogen, and sulfur were measured at the same time. Levels of barbituric acid-reactive substances, reduced glutathione and catalase. Results The volatile oil of cinnamon contained more than 98% cinnamaldehyde, which had an obvious dose-dependent protective effect on renal damage caused by alloxan. At the dose of 20 mg/kg, fasting blood glucose decreased the most.

4. Application No.: CN201410380545.X; Name of Invention: A pharmaceutical composition for the prevention and/or treatment of diabetes and its complications; Applicant: Yan Ming; Abstract: A new prevention and/or treatment of diabetes and its complications The pharmaceutical composition of the invention is made from peony and cinnamon, wherein the weight ratio of peony and cinnamon is 10-1:1. The active ingredients of the composition include 50-150 parts by weight of pinane monoterpene glycosides in Paeonia lactiflora and 1-5 parts by weight of cinnamic acid/aldehyde compounds. The pharmaceutical composition not only has good and stable hypoglycemic and weight loss effects, but also can effectively prevent and improve various diabetic complications, such as fatty liver, diabetic cardiomyopathy, diabetic peripheral neuritis, diabetic eye disease, and diabetic nephropathy. The composition has low toxicity, few side effects, low cost and safe use.

Cinnamon is widely used in diabetes and its complications. The contents disclosed in Documents 2 and 3, and Patent 4 are similar to the contents of the present invention. Although cinnamon is used for extraction, documents 2, 3, and patent 4 use methyl charles polyketone, cinnamon oil, and cinnamic acid/aldehyde, all of which are fat-soluble components of cinnamon, but the present invention uses defatted cinnamon for extraction. Therefore, the obtained extract is very different from documents 2 and 3, and patent 4.

5. Application number: CN201711331121; Name of invention: A compound composition of traditional Chinese medicine for lowering blood sugar; Applicant: Guangxi University of Traditional Chinese Medicine; Abstract: The invention relates to the field of natural medicines, in particular to a compound composition of traditional Chinese medicine for lowering blood sugar. The traditional Chinese medicine compound composition comprises the following pharmaceutical active ingredients by weight: total flavonoids of cinnamon 5-10 mg/g, cinnamon polyphenols 6-11 mg/g, proanthocyanidins 0.5-1.0 mg/g, Rehmannia glutinosa polysaccharide 10-16 mg/g, Rehmannia glutinosa Glycoside A 0.6-1.5mg/g, rehmangoside D 0.6-1.6mg/g, verbascoside 0.5-1.5mg/g and catalpol 0.4-7.2mg/g, the hypoglycemic traditional Chinese medicine composition product of the present invention is stable, It has the functions of increasing insulin sensitivity, promoting insulin secretion, lowering blood sugar, and at the same time regulating blood lipids and improving liver function; the composition of the present invention is widely used in the treatment and prevention of diabetes, diabetic complications and blood lipid regulation medicines or health products Applications.

6. Application No.: CN200510110763.2; Name of Invention: A pharmaceutical composition for treating diabetes or diabetic nephropathy and its preparation method; Applicant: Shanghai Institute of Materia Medica, Chinese Academy of Sciences; Abstract: The present invention discloses a pharmaceutical composition for treating diabetes or diabetic nephropathy The pharmaceutical composition and preparation method thereof. The medicinal composition is prepared by using the extracts of traditional Chinese medicines Phellodendri, Anemarrhena and cinnamon as effective medicinal components. It contains Phellodendron alkaloids, Zhimu glycosides, cinnamon volatile oil and polyphenols. The pharmaceutical composition of the present invention can be extracted from the single medicinal materials of Phellodendron chinensis, Anemarrhena and Cinnamon respectively and then mixed together, or the medicinal materials can be combined and extracted. The preparation method includes ethanol extraction, macroporous resin adsorption, elution, concentration, drying and other steps.

The compound ingredients disclosed in Patent 5 and Patent 6 all contain cinnamon polyphenols, but the content of cinnamon polyphenols is relatively low. The content limit in the composition is only ≥1%, and Patent 5 and Patent 6 do not specify the main composition type in the cinnamon polyphenols they use. At present, there is no report on the single use of cinnamon polyphenols with higher purity and clear main components in the preparation of foods and medicines for the adjuvant treatment of diabetic nephropathy.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a kind of application of the defatted cinnamon polyphenol extract extracted from cinnamon bark in the preparation of products for preventing and treating diabetic nephropathy, specifically, the application of the defatted cinnamon polyphenol extract in the preparation of reducing diabetic nephropathy kidney damage and preventing diabetic nephropathy New uses in related foods and medicines.

Further, the application of the defatted cinnamon polyphenol extract of the present invention in preparing a product for preventing and treating diabetic nephropathy, the main active ingredient of the defatted cinnamon polyphenol extract is one of cinnamon tannin and cinnamon epicatechin or The mixture of the two, one or both of cinnamon tannin and epicatechin extracted from cinnamon, can reduce urinary albumin, kidney index, blood urea nitrogen and blood urea in diabetic rats in vivo. creatinine. The structural formulas of cinnamon tannins and cinnamon epicatechin are as follows:

The mixture of cinnamon tannin and cinnamon epicatechin is mixed in any proportion.

The application of the above-mentioned defatted cinnamon polyphenol extract in the preparation of a product for preventing and treating diabetic nephropathy, the present invention also provides an extraction method for the defatted cinnamon polyphenol extract, which is specifically divided into two steps, namely, the first step of supercritical degreasing To obtain defatted cinnamon, the second step is solvent extraction of cinnamon polyphenol extract.

In the method for extracting the defatted cinnamon polyphenol extract, the specific steps for obtaining defatted cinnamon by supercritical degreasing in the first step are as follows: take the cinnamon bark and pulverize it, put it into a supercritical _CO extraction kettle, and extract it at a pressure of 25 to 45 MPa. Temperature 30℃~75℃, separation I pressure 10~30MPa, separation I temperature 25℃~75℃, separation II pressure 4~10MPa, separation II temperature 25℃~55℃, _CO flow 10~30kg per hour per kilogram of raw material , extract 2~10h, take out the defatted cinnamon from the extract. The specific steps of the second step of solvent extraction of cinnamon polyphenol extract are as follows: add ethanol with a volume fraction of 45 to 75% in a ratio of 1:1 to 1:9 times the weight of defatted cinnamon, until the sample is completely submerged, extract 1 to 3 1~10 h each time, the temperature was kept at 30 ℃~80 ℃, the filtrates were combined and concentrated, adsorbed on a DM130 macroporous resin column, washed with pure water and ethanol with a mass concentration of 10%~40% in turn, and then Elution is carried out with ethanol having a mass concentration of 40% to 80%, the eluate is collected, and the extract is obtained after drying under reduced pressure, wherein the total cinnamon polyphenol weight content is 40% to 80%.

In the defatted cinnamon polyphenol extract obtained by the above-mentioned extraction method of defatted cinnamon polyphenol extract, the total polyphenol weight content is 40% to 80%; wherein cinnamon tannin accounts for 30% of the total polyphenol weight content in the extract. %~55%; epicatechin accounts for 10%~30% of the total polyphenol weight content in the extract.

The application of the defatted cinnamon polyphenol extract extracted from cinnamon in the preparation of products for preventing and treating diabetic nephropathy includes the application of the defatted cinnamon polyphenol extract in preparing foods and medicines related to reducing diabetic nephropathy kidney damage and preventing diabetic nephropathy.

Defatted cinnamon polyphenol extract prevents and treats renal function in diabetic nephropathy, which refers to the effect of defatted cinnamon polyphenols (containing one or two mixtures of cinnamon tannin and cinnamon epicatechin) on urinary albumin, renal function of diabetic nephropathy patients. Index, serum urea nitrogen and the control of diabetic nephropathy-related indicators recognized by modern medicine.

Use of defatted cinnamon polyphenol extracts in the preparation of products for preventing and treating diabetic nephropathy, referring to the use of defatted cinnamon polyphenols (containing one or a mixture of cinnamon tannin and cinnamon epicatechin) in the preparation of improving diabetic nephropathy The use of related foods and drugs for the prevention of diabetic nephropathy and renal function.

Pharmacodynamic experiments of the present inventors show that in a rat model of diabetic nephropathy induced by high-fat feeding and low-dose streptozotocin, it is found that taking defatted cinnamon polyphenols for 8 weeks can significantly reduce urinary albumin and glycated hemoglobin , Glycated serum protein, renal index, blood urea nitrogen, serum creatinine, indicating that defatted cinnamon polyphenols have protective effects on the kidneys of diabetic nephropathy rats.

According to the above research of the inventor, the defatted cinnamon polyphenol extract provided by the present invention has the effect of improving the renal function of patients with diabetic nephropathy, and is expected to be used for the prevention and treatment of diseases such as diabetic nephropathy.

The invention has the following advantages: the method for extracting the defatted cinnamon polyphenol extract provided by the present invention is simple and easy to operate, and suitable for industrial production; experiments show that the defatted cinnamon polyphenol can significantly reduce the renal function damage of diabetic nephropathy patients; it has a good application prospect , the slag after industrial extraction of cinnamon oil can be used as the raw material source of defatted cinnamon, which not only makes full use of traditional Chinese medicine resources, but also ensures low cost of raw materials.

Detailed ways

In order to better illustrate the characteristics and essence of the present invention, the extraction method of defatted cinnamon polyphenols extract and the pharmacological experimental results of defatted cinnamon polyphenols improving the renal function of diabetic nephropathy rats are provided below in the form of examples, to illustrate its practical use, However, the specific use is not limited by the examples.

Example 1

Application of a defatted cinnamon polyphenol extract in the preparation of a product for preventing and treating diabetic nephropathy, the defatted cinnamon polyphenol extract comprises one or a mixture of cinnamon tannin and cinnamon epicatechin, cinnamon tannin and The structural formula of cinnamon epicatechin is as follows:

The mixture of cinnamon tannin and cinnamon epicatechin is mixed in any proportion.

A method for extracting a defatted cinnamon polyphenol extract is specifically divided into two steps, namely, the first step is supercritical degreasing to obtain defatted cinnamon, and the second step is solvent extraction of the cinnamon polyphenol extract. The concrete steps that the first step supercritical degreasing obtains degreasing cinnamon are as follows: get cinnamon bark and pulverize, pack into supercritical CO ₂ in extraction kettle, extraction pressure 25～45MPa, extraction temperature 30 ℃～75 ℃, separate 1 pressure 10～30MPa, Separation I temperature is 25°C~75°C, separation II pressure is 4~10MPa, separation II temperature is 25°C~55°C, _CO flow rate is 10~30kg per kilogram of raw material per hour, extraction is 2~10h, and defatted cinnamon is taken out from the extract. The specific steps of the second step of solvent extraction of cinnamon polyphenol extract are as follows: add ethanol with a volume fraction of 45 to 75% in a ratio of 1:1 to 1:9 times the weight of defatted cinnamon, until the sample is completely submerged, extract 1 to 3 1~10 h each time, the temperature was kept at 30 ℃~80 ℃, the filtrates were combined and concentrated, adsorbed on a DM130 macroporous resin column, washed with pure water and ethanol with a mass concentration of 10%~40% in turn, and then Elution with ethanol having a mass concentration of 40% to 80%, collecting the eluate, and drying under reduced pressure to obtain a defatted cinnamon polyphenol extract; in the obtained defatted cinnamon polyphenol extract, the total polyphenol weight content is 40% to 40%. 80%.

Example 2

A method for extracting a defatted cinnamon polyphenol extract is specifically divided into two steps, namely, the first step is supercritical degreasing to obtain defatted cinnamon, and the second step is solvent extraction of the cinnamon polyphenol extract. The concrete steps that the first step supercritical degreasing obtains degreasing cinnamon are as follows: get cinnamon bark and pulverize, pack into supercritical CO ₂ in the extraction kettle, extraction pressure 25MPa, extraction temperature 30 ℃～75 ℃, separation 1 pressure 10MPa, separation 1 temperature 25 ℃~75℃, separation II pressure 4MPa, separation II temperature 25℃~55℃, _CO2 flow rate 10kg per hour per kg of raw material, extraction for 10h, take out the defatted cinnamon from the extract. The specific steps of the second step of solvent extraction of cinnamon polyphenol extract are as follows: add 75% ethanol by volume 1 to 9 times the weight of defatted cinnamon until the sample is completely submerged, and extract once for 10 h each time , the temperature was kept at 30 ℃ ~ 80 ℃, the filtrates were combined and concentrated, adsorbed on a DM130 macroporous resin column, rinsed with pure water and 10% ethanol in turn, and then eluted with 40% ethanol, collected. The eluate was dried under reduced pressure to obtain a defatted cinnamon polyphenol extract.

Example 3

A method for extracting a defatted cinnamon polyphenol extract is specifically divided into two steps, namely, the first step is supercritical degreasing to obtain defatted cinnamon, and the second step is solvent extraction of the cinnamon polyphenol extract. The concrete steps that the first step supercritical degreasing obtains degreasing cinnamon are as follows: get cinnamon bark and pulverize, pack into supercritical _CO In the extraction kettle, extraction pressure 35MPa, extraction temperature 30 ℃～75 ℃, separation 1 pressure 20MPa, separation 1 temperature 25 ℃~75℃, separation II pressure 6MPa, separation II temperature 25℃~55℃, _CO2 flow rate 20kg per hour per kilogram of raw material, extraction for 8h, take out defatted cinnamon from the extract. The specific steps of the second step of solvent extraction of cinnamon polyphenol extract are as follows: add ethanol with a volume fraction of 60% in a ratio of 6 times the weight of defatted cinnamon, extract twice, and extract 7 hours each time, and keep the temperature at 30 ℃ ~ 80 ℃ , the filtrate was combined and concentrated, adsorbed with DM130 macroporous resin column, rinsed with pure water and 30% ethanol in turn, and then eluted with 60% ethanol, collected the eluate, and dried under reduced pressure to obtain Defatted cinnamon polyphenol extract.

Example 4

A method for extracting a defatted cinnamon polyphenol extract is specifically divided into two steps, namely, the first step is supercritical degreasing to obtain defatted cinnamon, and the second step is solvent extraction of the cinnamon polyphenol extract. The concrete steps that the first step supercritical degreasing obtains defatted cinnamon are as follows: get cinnamon bark and pulverize, pack into supercritical CO ₂ in the extraction kettle, extraction pressure 45MPa, extraction temperature 30 ℃～75 ℃, separation 1 pressure 30MPa, separation 1 temperature 25 ℃~75℃, separation II pressure 10MPa, separation II temperature 25℃~55℃, _CO2 flow rate 30kg per hour per kg of raw material, extraction for 2h, take out defatted cinnamon from the extract. The specific steps of the second step of solvent extraction of cinnamon polyphenol extract are as follows: add ethanol with a volume fraction of 45% according to the proportion of 9 times the weight of defatted cinnamon, and extract for 3 times, each time for 1 h, and the temperature is kept at 30 ℃ ~ 80 ℃ ℃, the filtrate was combined and concentrated, adsorbed with DM130 macroporous resin column, rinsed with pure water and 40% ethanol in sequence, and then eluted with 80% ethanol, collected the eluate, and dried under reduced pressure. A defatted cinnamon polyphenol extract was obtained.

Example 5

Protective effect of defatted cinnamon polyphenols on renal function in rats with diabetic nephropathy

1.1 Reagents: defatted cinnamon polyphenols: the defatted cinnamon polyphenols extract obtained by extracting the defatted cinnamon polyphenol extracts according to the extraction method of the defatted cinnamon polyphenol extracts in Example 3 was obtained by using cinnamon as a raw material. Streptozotocin (STZ), a product of SIGMA; Irbesartan tablets, Sanofi (Hangzhou) Pharmaceutical Co., Ltd.; Blood glucose test strips, produced by Roche Diagnostics (Shanghai) Co., Ltd.; High-fat feed formula: basic feed 74.5 %, cholesterol 1%, egg yolk powder 5%, sucrose 10%, lard 9%, sodium cholate 0.5%; rat urine microalbumin (MAU) enzyme-linked immunosorbent assay kit, Wuhan Elite Biotechnology Co., Ltd.; urea nitrogen kit and creatinine kit, produced by Nanjing Jiancheng Bioengineering Institute.

1.2 Experimental animals: SD rats, all male, weighing 180-220 g, purchased from Hunan Slike Jingda Laboratory Animal Co., Ltd.

1.3 Experimental method: A number of SD male rats were taken and fed with high-fat feed, and a blank group was set up and fed with normal feed. After 6 weeks, all rats outside the blank group were injected intraperitoneally with streptozotocin 35 mg/Kg to make a model, the blank group was injected with the same amount of sodium citrate buffer, and blood glucose was measured once 72 hours after the injection of streptozotocin , blood sugar >11.1 mmol/L. Rats with successful modeling were randomly and evenly divided into 5 groups according to their blood glucose values: model group, high defatted cinnamon polyphenols (400 mg/kg), defatted cinnamon polyphenols (200 mg/kg), and defatted cinnamon polyphenols (100 mg/kg). /kg) three treatment groups, irbesartan treatment group (30 mg/kg). The administration group was given ig, once a day, the blank group and the model group were given the same amount of normal saline for 8 consecutive weeks, the blank group was given ordinary feed during the experiment, and the model group and each drug treatment group were given high-fat feed. feed.

During the administration period, fasting blood glucose levels were measured at 2, 4, 6, and 8 weeks after administration. On the 8th week of administration, 24h urine was collected to measure urinary albumin. After 8 weeks of administration, the patients were anesthetized with 10% chloral hydrate, and blood was collected from the abdominal aorta to detect glycosylated hemoglobin according to the kit method. Serum creatinine, blood urea nitrogen, glycated serum protein. Both kidneys were taken and weighed to calculate the kidney index.

1.4 Statistical analysis: The experimental results are expressed as mean ± standard deviation. Differences between groups were analyzed by t test.

1.5 Experimental results: During the drug treatment period, the fasting blood glucose value of the model group has been maintained at a high level. From the 4th week of administration, compared with the model group, the defatted cinnamon polyphenol high-dose group had a significant hypoglycemic effect (P<0.05), and the effect lasted until the 8th week (P<0.05, which was similar to the model group). After 6 weeks of administration, both the middle-dose and low-dose defatted cinnamon polyphenols had significant hypoglycemic effects (P<0.05), which also lasted to 8 weeks after administration. It shows that defatted cinnamon polyphenol has a stable hypoglycemic effect on diabetic nephropathy rats. The data are shown in Table 1.

The detection of glycated serum protein in the blood of diabetic nephropathy rats showed that compared with the model group, each dose of defatted cinnamon polyphenols could significantly reduce the level of glycated serum protein (P<0.01 or P<0.05). In the determination of glycated hemoglobin, it was found that compared with the model group, defatted cinnamon polyphenols 400mg/kg had a significant reduction effect on the glycated hemoglobin of diabetic nephropathy rats (P<0.05). Decreased glycated hemoglobin. The data are shown in Table 2.

By collecting the 24h urine of the animals and measuring the urinary albumin, it was found that compared with the model group, each dose of defatted cinnamon polyphenols could significantly reduce the urinary albumin (p<0.01). It shows that defatted cinnamon polyphenols can effectively improve the renal filtration damage in animals with diabetic nephropathy and play a role in renal protection. The experimental data are shown in Table 3.

In order to further verify the renal protective effect of cinnamon polyphenols, we detected the renal index of animals in each group, and found that defatted cinnamon polyphenols 200mg/kg could reduce the renal index (P<0.05). The data are shown in Table 4.

In the blood of diabetic nephropathy rats, when measuring blood urea nitrogen and serum creatinine, it was found that compared with the model group, defatted cinnamon polyphenols could significantly reduce the levels of blood urea nitrogen and serum creatinine at each dose (P<0.01). The data are shown in Table 5.

1.6 Conclusion: The inventors' experiments found that defatted cinnamon polyphenols can significantly alleviate the fasting blood sugar increase in diabetic nephropathy rats, and significantly reduce glycated serum protein and glycated hemoglobin, and at the same time can reduce kidney index, urine albumin, blood urea. Nitrogen and serum creatinine, indicating that defatted cinnamon polyphenols can alleviate kidney damage in rats with diabetic nephropathy, and have the effect of preventing and treating diabetic nephropathy.

Claims (7)

1. Application of defatted cinnamon polyphenol extract in the preparation of products for preventing and treating diabetic nephropathy. 2. the application of the defatted cinnamon polyphenol extract according to claim 1 in the preparation of products for preventing and treating diabetic nephropathy, it is characterized in that: the described defatted cinnamon polyphenol extract has the functions of relieving diabetic nephropathy kidney damage and preventing diabetic nephropathy in preparation related food and drug uses. 3. the application of the defatted cinnamon polyphenol extract according to claim 1 and 2 in the preparation of products for preventing and treating diabetic nephropathy, it is characterized in that: described defatted cinnamon polyphenol extract comprises cinnamon tannin and cinnamon epicatechin One or a mixture of both, cinnamon tannins and cinnamon epicatechin have the following structural formulas: The mixture of cinnamon tannin and cinnamon epicatechin is mixed in any proportion. 4. the application of the defatted cinnamon polyphenol extract according to claim 3 in the preparation of products for preventing and treating diabetic nephropathy, it is characterized in that: the extraction method of the described defatted cinnamon polyphenol extract is divided into two steps, respectively, One-step supercritical degreasing to obtain defatted cinnamon, and the second-step solvent extraction of cinnamon polyphenol extract. 5. the application of the defatted cinnamon polyphenol extract according to claim 4 in the preparation of products for preventing and treating diabetic nephropathy, it is characterized in that: in the extraction method of the described defatted cinnamon polyphenol extract, the first step of supercritical degreasing obtains The concrete steps of defatted cinnamon are as follows: get cinnamon bark and pulverize, pack into supercritical CO ₂ in extraction kettle, extraction pressure 25～45MPa, extraction temperature 30 ℃～75 ℃, separate 1 pressure 10～30MPa, separate 1 temperature 25～75 ℃ ℃, separation II pressure 4~10MPa, separation II temperature 25℃~55℃, _CO2 flow rate 10~30kg per hour per kilogram of raw material, extraction 2~10h, take out defatted cinnamon from the extract. 6. the application of the defatted cinnamon polyphenol extract according to claim 4 in the preparation of products for preventing and treating diabetic nephropathy, it is characterized in that: in the extraction method of the described defatted cinnamon polyphenol extract, the second step solvent extracts cinnamon polyphenols The specific steps of the phenolic extract are as follows: add ethanol with a volume fraction of 45 to 75% at a ratio of 1:1 to 1:9 times the weight of the defatted cinnamon, until the sample is completely submerged, extract 1 to 3 times, and extract 1 to 1 For 10 h, the temperature was kept at 30 ℃ ~ 80 ℃, the filtrates were combined and concentrated, adsorbed on a DM130 macroporous resin column, rinsed with pure water and ethanol with a mass concentration of 10% ~ 40% in turn, and then with a mass concentration of 40% ~ Elution with 80% ethanol, collecting the eluate, and drying under reduced pressure to obtain an extract, wherein the total cinnamon polyphenol weight content is 40% to 80%. 7. The application of the defatted cinnamon polyphenol extract according to claim 4 in the preparation of a product for preventing and treating diabetic nephropathy, characterized in that: the defatted cinnamon polyphenol extraction obtained by the extraction method of the described defatted cinnamon polyphenol extract is extracted. In the extract, the total polyphenol weight content is 40% to 80%; wherein cinnamon tannin accounts for 30% to 55% of the total polyphenol weight content in the extract; epicatechin accounts for 10% of the total polyphenol weight content in the extract. %~30%.