CN114588184A

CN114588184A - Potentilla anserine extract and preparation method and application thereof

Info

Publication number: CN114588184A
Application number: CN202011397666.7A
Authority: CN
Inventors: 唐亮; 严发展; 丁滨
Original assignee: Hangzhou Zhimo Biotechnology Co ltd
Current assignee: Hangzhou Zhimo Biotechnology Co ltd
Priority date: 2020-12-03
Filing date: 2020-12-03
Publication date: 2022-06-07
Anticipated expiration: 2040-12-03
Also published as: CN114588184B

Abstract

The invention discloses a small coleus blumei extract and a preparation method and application thereof. The coleus tomentosa extract comprises active sites such as flavonoid compounds, organic acid compounds, ester compounds and the like. The invention can obviously promote cell proliferation, enhance the synthesis of ATP by cell energy metabolism, and simultaneously activate CAT enzyme to prevent oxidative damage generated in the energy metabolism process. Therefore, the coleus blumei extract can be used as a functional component of medicines, health-care products and daily chemical products for promoting cell proliferation and enhancing cell energy metabolism.

Description

Potentilla henryi extract and preparation method and application thereof

Technical Field

The invention belongs to the field of chemistry, and particularly relates to separation of an active site of a small coleus blumei and application of the active site in promoting cell proliferation.

Background

The coleus is a large genus of pteridophyte in the water-dragon orthopaedics, and the basic plants are mainly coleus macrophyllus, coleus angularis, coleus polystachyus and the like. The whole grass of the small Chinese swertia herb can be used as a medicine, has the functions of clearing heat and removing toxicity, and relieving swelling and pain, is widely applied in folks, and is generally used as tea to treat various inflammations. The research shows that the small lysimachia christinae hance can accelerate the body temperature reduction of a febrile to shorten the course of disease when being used for treating respiratory tract infection diseases. The small coleus blumei is rich in various organic acids, esters and flavonoid compounds, and has certain activity of resisting neuritis, cancer and the like.

Without cell proliferation, there are no multicellular organisms on earth. In the process of cell proliferation, not only are large amounts of amino acids, carbohydrates and nucleotides required, but also large amounts of energy are required, wherein Adenosine Triphosphate (ATP) is one of the main sources of energy required for some vital activities. Most multicellular organisms normally acquire ATP by respiration. The peroxide produced during this process is scavenged by Catalase (CAT), protecting the cells from peroxide damage.

Therefore, the research on the active site and the efficacy of the small coleus blumei has wide application prospect.

Disclosure of Invention

In order to realize the purpose of the invention, the invention provides a small coleus blumei extract and a preparation method and application thereof.

The coleus floridulus is named as a traditional Chinese medicine and refers to plant varieties of coleus genus (ophioglosum Linn.) in the coleaceae family, including coleus floridulus L., leptospirillum angustifolia L., and coleus cordifolia L., and also includes coleus cuspidatus Desv., coleus blunted kovar floridum Hook, coleus floridulus Grev. and other varieties of coleus including O.pendula, O.ailchisonii, O.pusillus, O.palustum, praeloglottis, and coleus japonica.

According to a first aspect, the invention provides a small goldenrod herb extract, which comprises 39.98-54.40% of total flavone compounds, 17.11-69.92% of total organic acid compounds and 13.16-38.09% of ester compounds by weight.

In some embodiments, the coleus tomentosa extract contains 40% to 52%, preferably 45% to 50%, by total weight of total flavonoids.

Further, the common goldenrod herb extract contains 14.70-30.92% of flavonoid glycoside compounds in total weight.

In some embodiments, the comfrey extract contains 20% to 60%, preferably 30% to 45%, by total weight of total organic acid compounds.

Further, the common goldenrod herb extract contains 1.30-17.95% of fatty acid compounds by total weight.

In some embodiments, the coleus tomentosa extract contains 15% to 35%, preferably 20% to 30%, by total weight of total ester compounds.

In a second aspect, the present invention provides a method of preparing an extract of comfrey, the method comprising the steps of: reflux-extracting herba Chloranthi Henryi with ethanol water solution, concentrating the extractive solution, extracting with ether solvent, discarding ether extractive solution, extracting with ester solvent, collecting ester extractive solution, and drying to obtain herba Chloranthi Henryi extract.

In some embodiments, the method further comprises the steps of drying and pulverizing before extracting the coleus blumei.

Preferably, the drying temperature is from 35 ℃ to 50 ℃, more preferably from 40 ℃ to 45 ℃.

Preferably, the powder is crushed to 40-80 meshes, and more preferably 60-80 meshes.

In some embodiments, reflux extraction with 60% to 95% aqueous ethanol; preferably, reflux extraction is carried out for 2-3 times; further preferably, each extraction is carried out for 0.5 to 1.5 hours. Preferably, the ratio of the volume of the 60-95% ethanol aqueous solution to the mass of the coleus blumei extract is (10-30) to 1.

In some embodiments, the volume ratio of the extraction solvent to the concentrate is (1-2) to 1, preferably 1: 1.

In some embodiments, each extraction solvent is extracted 3 to 5 times.

In some embodiments, the ethereal solvent is selected from one or more of methyl ether, ethyl ether, and petroleum ether; preferably petroleum ether.

In some embodiments, the ester solvent is selected from one or more of ethyl formate, ethyl acetate, ethyl propionate, or ethyl butyrate; preferably ethyl acetate.

In some embodiments, the method of preparing a coleus alternifolius extract of the present invention comprises the steps of: (1) pulverizing dried whole plant of Chlorella sorrel to 40-80 mesh, adding 60-95% ethanol water solution, extracting under reflux twice with boiling for 0.5-1.5 hr each time, mixing extractive solutions, and performing rotary evaporation on the filtrate under reduced pressure to obtain Chlorella sorrel crude extract concentrated solution; (2) adding petroleum ether into the concentrated solution according to the volume of 1: 1, extracting for 3-5 times, and discarding the extract; (3) adding ethyl acetate into the concentrated solution extracted by petroleum ether according to the volume of 1: 1, extracting for 3-5 times, combining the extracts, and performing rotary evaporation under reduced pressure to obtain a dry small coleus blumei extract.

In a third aspect, the present invention provides a coleus tomentosa extract prepared by the method.

The active site of the coleus blumei extract obtained by the preparation method of the present invention is not a single compound, but includes a combination of all compounds in the extract obtained by the preparation method.

Preferably, the common goldenrod herb extract contains 39.98-54.40% of total flavone compounds, 17.11-69.92% of total organic acid compounds and 13.16-38.09% of ester compounds by total weight.

In some embodiments, the comfrey extract contains 40% to 52%, preferably 45% to 50% total flavonoids by total weight.

In a fourth aspect, the invention provides application of the small coleus blumei extract in preparing medicines, health-care products and daily chemical products for promoting cell proliferation.

In some embodiments, the promoting cell proliferation comprises promoting proliferation of HaCaT and/or HOEC cells, and/or increasing transcription of structural proteins COL1a1 and FN1 and the cytokine TNF-a in the cells.

In a fifth aspect, the invention provides application of the coleus blumei extract in preparing medicines, health-care products and daily chemical products for enhancing cell energy metabolism.

In some embodiments, the enhancing cellular energy metabolism comprises increasing intracellular ATP concentration, and/or increasing intracellular CAT enzyme activity.

In a sixth aspect, the present invention provides the use of the coleus tomentosa extract for the preparation of a medicament, health product, daily chemical product for promoting hair growth or regrowth and/or eyelash growth.

In a seventh aspect, the invention provides application of the coleus deltoidea extract in preparing medicines, health products and daily chemical products for promoting wound healing.

The coleus blumei extract can be mixed with auxiliary materials accepted by medicaments, daily chemical products and food to prepare various dosage forms such as capsules, tablets, powder, liniment, soft capsules, oral liquid, pills, gels, emulsions, essential oil, sustained release agents and the like which mainly comprise the coleus blumei extract by modern mixing and preparation technology.

The invention has the beneficial effects that: the invention of the small coleus extract can be 1: promoting cellular energy metabolism, and showing that the ATP content and CAT enzyme activity in cells are increased; 2: the functional components of medicines, health products and daily chemical products for promoting cell proliferation, such as hair growth, wound healing, eyelash growth, etc., can promote cell proliferation, and increase the cell number, the cell proliferation-related cytokine TNF-a, and the transcription of the structural protein FN1 and COL1A 1.

Drawings

FIG. 1 is a flow chart of the process for preparing the extract of Potentilla henryi of the present invention.

FIG. 2 is a UPLC-Q/TOF-MS analysis chart of the extract of Potentilla discolor of the present invention.

FIG. 3 shows the cell proliferation promoting activity of the extract of Potentilla discolor of the present invention.

FIG. 4 shows that the extract of geldanella vulgaris of the present invention promotes the transcription of COL1A1 and FN1 as well as TNF-a.

FIG. 5 shows that the extract of Potentilla fragrans of the present invention promotes cellular ATP synthesis.

FIG. 6 shows that the extract of Potentilla discolor of the present invention enhances the activity of CAT enzyme.

FIG. 7 shows that the invention of Meyer sedge promotes the hair regeneration in the mouse depilation region.

Detailed Description

To further illustrate the embodiments of the present invention, the present invention is further described below with reference to the following examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the present invention, the raw materials, reagents and equipment used are all commercially available or commonly used in the art, unless otherwise specified.

Example 1

This example provides a comfrey extract prepared by a process comprising the steps of:

(1) collected whole grass of Potentilla chinensis (Ophioglossum vulgatum L.) was oven-dried at 45 deg.C, pulverized to 60 mesh, and stored in a cool and ventilated place.

(2) Placing 5g of the small glechoma herb powder into a 200mL round-bottom flask, adding 75 vol% ethanol according to the mass-volume ratio of 20 times, carrying out micro-boiling reflux extraction twice for 1 hour each time, and combining the extracting solutions.

(3) Concentrating the extractive solution by rotary evaporation under reduced pressure until no alcohol smell exists, adding water until the volume of the concentrated solution is 100mL, adding petroleum ether with the same volume according to the volume ratio of 1: 1 for extraction, discarding the extractive solution, and continuously extracting for 3 times until the extractive solution is colorless.

(4) Extracting with equal volume of ethyl acetate for 3 times until the extractive solution is colorless, and mixing the extractive solutions.

(5) Drying the extractive solution by rotary evaporation under reduced pressure to obtain herba Lysimachiae Capillipedis extract, and placing into a dryer for use.

The process flow described above is summarized as figure 1.

The extraction efficiency of the extract prepared from the dry powder of the Potentilla discolor is 1.67 + -0.77% (g/g Potentilla discolor powder).

The obtained herba Lysimachiae Foenumgraeci extract contains total flavone compounds 46.47 + -3.50% and flavonoid glycosides 22.70 + -4.53%; the content of total organic acid is 41.07 plus or minus 3.93 percent, and the content of fatty acid compounds is 13.85 plus or minus 3.46 percent; the content of the ester compound is 23.83 +/-5.30%.

Example 2

(1) collected whole grass of Potentilla furcifera (O.pedunculus Desv.) was oven-dried at 35 deg.C, pulverized to 40 mesh, and stored in a cool and ventilated place.

(2) Placing 5g of the brevifolius powder into a 200mL round-bottom flask, adding 95 vol% ethanol according to the mass-volume ratio of 10 times, performing micro-boiling reflux extraction for three times, each time for 0.5 hour, and combining the extracting solutions.

(3) Concentrating the extractive solution by rotary evaporation under reduced pressure until no alcohol smell exists, adding water until the volume of the concentrated solution is 100mL, adding petroleum ether at a volume ratio of 1: 1.5, extracting, discarding the extractive solution, and continuously extracting for 3 times until the extractive solution is colorless.

(4) Adding ethyl acetate according to the volume ratio of 1: 1.5 for extraction, continuously extracting for 3 times until the extract liquor is colorless, and combining the extract liquor.

(5) Drying the extract by rotary evaporation under reduced pressure to obtain the extract of the small coleus blumei.

The extraction efficiency of the extract prepared from the dry powder of the Potentilla discolor is 0.80 + -0.91% (g/g Potentilla discolor powder).

The obtained herba Lysimachiae Foenumgraeci extract contains 44.21 + -4.23% total flavonoids, and further contains 17.75 + -3.05% flavonoid glycosides; the content of the total organic acid is 23.70 +/-3.70 percent, and further, the content of the fatty acid compound is 5.56 +/-4.26 percent; the content of the ester compound is 18.98 +/-3.98%.

Example 3

(1) collected whole grass of Callicarpa angustifolia (O.thermal Kom.) was oven-dried at 50 deg.C, pulverized to 80 mesh, and stored in a cool and ventilated place.

(2) Placing 5g of the brevifolius powder into a 200mL round-bottom flask, adding 60 vol% ethanol according to the mass-volume ratio of 30 times, performing micro-boiling reflux extraction twice for 1.5 hours each time, and combining the extracting solutions.

(3) Concentrating the extractive solution by rotary evaporation under reduced pressure until no alcohol smell exists, adding water to volume of the concentrated solution to 100mL, adding petroleum ether at volume ratio of 1: 1, extracting, discarding the extractive solution, and continuously extracting for 4 times until the extractive solution has no color.

(4) Adding ethyl acetate according to the volume ratio of 1: 1 for extraction, continuously extracting for 4 times until the extract liquor is colorless, and combining the extract liquor.

The extraction efficiency of the extract prepared from the dry powder of the coleus blumei is 2.64 + -0.132% (g/g coleus blumei powder).

The obtained herba Lysimachiae Foenumgraeci extract contains 46.30 + -5.70% total flavonoids, and further contains 26.63 + -4.29% flavonoid glycosides; the content of the total organic acid is 39.45 plus or minus 3.36 percent, and further, the content of the fatty acid compounds is 12.42 plus or minus 5.53 percent; the content of the ester compound is 26.80 +/-3.20%.

Experimental example 1

1. The activity verification of the small coleus extract for enhancing cell energy metabolism and promoting cell proliferation.

1.1 Experimental materials

HaCaT cells (human immortalized keratinocytes) and HOEC cells (human oral epithelial cells) were purchased from a national laboratory cell resource sharing platform. Organic reagents such as DMSO (from east China medicine); fetal bovine serum and DMEM high-glucose medium (Sigma aldrich); ATP kit, CAT kit (manufactured by Nanjing); cDNA synthesis kit, PCR reaction system and SYBR GREEN (Dalianbao organism); COL1A1, FN-B1, GAPDH upstream and downstream primers (Shanghai Producer).

1.2 Experimental drugs

The extract of Potentilla discolor prepared in the above example 1 was dissolved in DMSO to prepare a stock solution of 10 mg/mL.

1.3 Experimental instruments

HH-2 digital display constant temperature water bath (Honghua instrument factory in Jiangsu Jintan city); WH-2 micro vortex mixer (Shanghai Huxi Analyzer); BP211D electronic analytical balance (Sartorius AG); water high performance liquid chromatograph; ultra-micro uv spectrophotometer (Quawell Q5000); rotary evaporator (sienna super jie).

1.4 Experimental methods

UPLC-Q/TOF-MS analysis: dissolving the herba Centellae extract with methanol, and analyzing the compound composition in 4 μ L methanol solution of herba Centellae extract with concentration of 1mg/mL by UPLC-Q-TOF-MS system. Chromatographic conditions are as follows: the chromatographic column is ACQUITY UPLC BEH C18 (150X 2.1mm, 1.7 μm), and the mobile phase is a composite system of solution A (acetonitrile containing 0.1% formic acid) and solution B (0.1% formic acid) mixed according to a certain concentration gradient along with time, and the specific conditions are as follows: 15% -20% of A in 0-5 min; 20-40% A in 5-32 min; 32-37 minutes 40-95% A. The flow rate was 0.3 mL/min. Analyzing chemical components in the oenocarpus chinensis extract by adopting a turbine ion spray ion source and an ESI anion-cation scanning mode. The concrete conditions are as follows: ion source Gas 1(Gas 1): 55, ion source Gas 2(Gas 2): 55, curtain gas (Cur): 35, source temperature: ionic phase voltage float (ISV F) at 60 ℃,: 5500v/4500 v; TOFMS scans m/z range: 50-1500 da, production scan m/z range: 50-1000 da, the MS scanning accumulation time is 0.25 s/spectrum, and the product ion scanning accumulation time is 0.05 s/spectrum; secondary mass spectrometry used IDA and high sensitivity mode, attenuation potential (DP): 60v (positive and negative ion mode). The obtained molecular weight is matched with a secondary data spectrum comprising 1500 Chinese herbal medicines through SCIEXOS software, and the analysis and identification of the compound are completed.

And (3) cell concentration determination: HaCaT cells were plated at 1.0X 10⁴Inoculating the strain/well into 96-well plate containing 10% fetal calf serum and 90% DMEM high-sugar medium at 37 deg.C and 5% CO₂Culturing in a cell culture box. The following day, incubation was continued for 14-16 hours with freshly prepared media containing varying concentrations of the coleus blumei extract (DMSO solution) as planned, with 6 replicates for each drug concentration. Cell concentrations were determined using MTT kit according to the instructions.

ATP concentration and CAT activity assay: HaCaT cells were plated at 5.0X 10⁵Cells were cultured and treated in 24-well plates at individual/mL concentrations, and the intracellular ATP levels and CAT activities were determined according to the methods provided in the ATP and CAT assay kits.

Real-Time PCR: HaCaT cells were plated at 5.0X 10⁵The cells were cultured and treated in the same manner by inoculating 24-well plates at a concentration of one/mL. After the collected cells are washed by PBS solution, total RNA is extracted by a Trizol-chloroform method, 1 microliter of RNA sample is added with 99 microliter of DEPC water and is fully and uniformly mixed, the concentration of RNA in the sample is measured, and a reverse transcription system is prepared according to the RNA concentration. After RNA is subjected to reverse transcription, a 12.5 mu L real-time PCR amplification system is prepared; the reaction system was activated at 95 ℃ for 3min and then 45 cycles of denaturation at 95 ℃ for 2 sec, annealing at 60 ℃ and extension for 30 sec were performed. The primer sequences used are listed in table 1 below:

TABLE 1 primer sequences

Westem blot: HaCaT cells were plated at 5.0X 10⁵The cells were cultured and treated in the same manner by inoculating 24-well plates at a concentration of one/mL. Collecting cells, washing with PBS solution, adding PBS solution, and performing ultrasonic cleavageAfter cell lysis, cell debris was removed by centrifugation. After the protein concentration of the supernatant was quantified by the Bradford method, SDS Loading buffer (Loading buffer) was prepared at a concentration of 40. mu.g of protein. After the protein is separated by 10% SDS-PAGE, the protein is electrically transferred to a cellulose acetate membrane, and after 2 hours of skim milk blocking, the protein is incubated with the antibody overnight for the next day of detection.

1.5 animal handling and smearing experiments

After applying depilatory cream to the two-flank region of an 8-week-old female BALB/c mouse, the mouse was washed with sterile distilled water 2 times for 3 minutes each. The skin was moisturized with 1mg/mL of the extract of Potentilla chinesis and an equal volume of distilled water, 6 times a day. Anesthetized mice measured both side bare skin surface areas after 14 days.

1.6 statistics and analysis

Measure data results to

T-test was used for the differences between groups.

2. Results

FIG. 1 is a schematic diagram showing a process for preparing a Potentilla anserine extract according to the present invention; the extraction efficiency of the extract prepared from the dry powder of coleus blumei according to the method of example 1 was 1.67 ± 0.77% (g/g coleus blumei powder).

FIG. 2 shows UPLC-Q/TOF-MS analysis chart of the extract of Potentilla chinense of the present invention.

Through comparison and screening with the primary accurate mass number, isotope distribution ratio and MS/MS of a compound TCM MS/MS Library in a traditional Chinese medicine secondary database carried by SCIEX OS software, 15 chromatographic peaks (shown in a table 2) are assigned in a positive ion mode (shown in a figure 2A), and 22 chromatographic peaks (shown in a table 3) are assigned in a negative ion mode (shown in a figure 2B).

TABLE 2 chemical composition of the Potentilla extract in positive ion mode

TABLE 3 chemical composition of the Potentilla extract in negative ion mode

FIG. 3 shows that the extract of Coleus forskohlii of the present invention can promote the proliferation of both HaCaT (FIG. 3A) and HOEC (FIG. 3B) cells; it was shown that when the extract concentration was less than 125. mu.g/mL, the cell concentration in each concentration gradient treated group was increased compared to the untreated control group (Ct), with the two concentration effects of 62.5. mu.g/mL and 125. mu.g/mL being significant and very significant, respectively.

FIG. 4 shows that the extract of geldanum elegans of the present invention promotes the transcription of structural proteins COL1A1 and FN1 and cell proliferation regulatory factor TNF-. alpha.in cells; transcription of the structural proteins COL1A1 and FN1 in cells was very significantly elevated (p < 0.01) at concentrations of between 15. mu.g/mL and 60. mu.g/mL of the P.insubstantial extract. COL1a1 and FN1 are the coding genes for collagen type I and fibronectin, respectively, and expression of these two proteins indicates vigorous cell proliferation. The cell proliferation regulatory factor TNF-alpha is often involved in the proliferation of cells, and the intracellular TNF-alpha transcription is remarkably increased when the concentration of the Megalobrass extract is between 15 mu g/mL and 60 mu g/mL, and the TNF-alpha transcription is also an important evidence for vigorous cell proliferation.

FIG. 5 shows that the extract of Chlorella sorokiniana according to the present invention is capable of promoting cellular ATP synthesis; the intracellular ATP concentration was very significant (p < 0.001) and significantly increased (p < 0.05) at concentrations of the P.juncea extract of 7.5. mu.g/mL and 15. mu.g/mL, while the ATP concentration was somewhat but not significant at concentrations greater than 30. mu.g/mL.

FIG. 6 shows that the extract of Chlorella sorokiniana according to the present invention enhances the CAT enzyme activity in cells; the intracellular CAT enzyme activity was very significant (p < 0.001) and significantly increased (p < 0.05) at concentrations of the P.deltoidea extract of 30. mu.g/mL and 60. mu.g/mL. And the activity is reduced with the increase of the concentration.

FIG. 7 shows that the invention of the extract of Potentilla fragrans promotes hair regrowth in the mouse's depilatory area; application of the extract to the depilatory region (right side) of mice at a concentration of 1mg/mL of the extract of Chlorella mellea significantly promoted hair regrowth in the depilatory region (P < 0.05), as shown in Table 4.

Table 4 the extract of veronica peregrina of the present invention promotes hair regrowth in the mouse's hair removal zone

Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

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Claims

1. The small coleus extract is characterized by comprising 39.98-54.40 percent of total flavone compounds, 17.11-69.92 percent of total organic acid compounds and 13.16-38.09 percent of ester compounds by total weight.

2. A method of preparing a comfrey extract, comprising the steps of: reflux-extracting herba Chloranthi Henryi with ethanol water solution, concentrating the extractive solution, extracting with ether solvent, discarding ether extractive solution, extracting with ester solvent, collecting ester extractive solution, and drying to obtain herba Chloranthi Henryi extract.

3. The method according to claim 2, further comprising the steps of drying and pulverizing before extracting the coleus blumei;

preferably, the drying temperature is 35-50 ℃, and/or the crushing is carried out to 40-80 meshes.

4. The method according to claim 2 or 3, characterized in that the reflux extraction is carried out with 60-95% aqueous ethanol; preferably, reflux extraction is carried out for 2-3 times; further preferably, each extraction is carried out for 0.5 to 1.5 hours.

5. The method according to claim 2 or 3, wherein the ether solvent is selected from one or more of methyl ether, ethyl ether and petroleum ether; preferably petroleum ether;

and/or the ester solvent is selected from one or more of ethyl formate, ethyl acetate, ethyl propionate or ethyl butyrate; preferably ethyl acetate.

6. An extract of Potentilla chinense prepared by the method of any one of claims 2-5.

7. Use of the coleus tomentosa extract of claim 1 or 6 for the preparation of a medicament, health product, daily chemical product for promoting cell proliferation;

preferably, said promoting cell proliferation comprises promoting proliferation of HaCaT and/or HOEC cells, and/or increasing transcription of structural proteins COL1a1 and FN1 and the cytokine TNF- α in cells.

8. Use of the coleus tomentosa extract of claim 1 or 6 for the preparation of a medicament, health product, daily chemical product for enhancing energy metabolism of cells;

preferably, the enhancing cellular energy metabolism comprises increasing intracellular ATP concentration, and/or increasing intracellular CAT enzyme activity.

9. Use of the Potentilla anserine extract according to claim 1 or 6 for the preparation of a medicament, health product, daily chemical product for promoting hair growth or regrowth and/or eyelash growth.

10. Use of the coleus tomentosa extract according to claim 1 or 6 for the preparation of a medicament, a health product, a daily chemical product for promoting wound healing.