ES2498790B2 - Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra - Google Patents
Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra Download PDFInfo
- Publication number
- ES2498790B2 ES2498790B2 ES201330398A ES201330398A ES2498790B2 ES 2498790 B2 ES2498790 B2 ES 2498790B2 ES 201330398 A ES201330398 A ES 201330398A ES 201330398 A ES201330398 A ES 201330398A ES 2498790 B2 ES2498790 B2 ES 2498790B2
- Authority
- ES
- Spain
- Prior art keywords
- analyte
- sample
- electrochemical
- compound
- magneto
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000012491 analyte Substances 0.000 title abstract 4
- 238000000835 electrochemical detection Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000005406 washing Methods 0.000 title abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 238000011534 incubation Methods 0.000 abstract 2
- 239000006249 magnetic particle Substances 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000002848 electrochemical method Methods 0.000 abstract 1
- 238000006479 redox reaction Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
Abstract
La invención describe un procedimiento rápido, simple y eficaz para la detección magneto-electroquímica de un analito en una muestra eliminando todo paso de lavado, que comprende la incubación de dicha muestra en presencia de partículas magnéticas y de un elemento de reconocimiento ligado a un enzima en que dicho elemento de reconocimiento y dichas partículas magnéticas son capaces de interaccionar independientemente con dicho analito, la aplicación del electrodo al resultado de esta etapa de incubación en presencia de al menos un imán, la adición de un sustrato electroquímico de dicho enzima para obtener un compuesto con capacidad óxido-reductora, la medición electroquímica en la superficie del electrodo de dicho compuesto con capacidad óxido-reductora o de al menos un producto de una reacción redox generada por dicho compuesto en presencia de los componentes de la muestra, los reactivos añadidos en la incubación y del sustrato electroquímico, y la determinación de la presencia del analito evaluando el resultado de la etapa anterior respecto de una referencia.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201330398A ES2498790B2 (es) | 2013-03-20 | 2013-03-20 | Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra |
| PCT/EP2014/055367 WO2014147049A1 (en) | 2013-03-20 | 2014-03-18 | No-wash magneto-electrochemical procedure to detect an analyte in a sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES201330398A ES2498790B2 (es) | 2013-03-20 | 2013-03-20 | Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2498790A1 ES2498790A1 (es) | 2014-09-25 |
| ES2498790B2 true ES2498790B2 (es) | 2015-06-25 |
Family
ID=50382431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES201330398A Expired - Fee Related ES2498790B2 (es) | 2013-03-20 | 2013-03-20 | Procedimiento para la detección magneto-electroquímica sin lavados de un analito en una muestra |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES2498790B2 (es) |
| WO (1) | WO2014147049A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3067116A1 (en) | 2015-03-13 | 2016-09-14 | Fundació Hospital Universitari Vall d' Hebron - Institut de Recerca | Device for electrochemical detection with magnetic particles |
| US10670595B2 (en) * | 2015-05-26 | 2020-06-02 | Oncogenesis, Inc. | System, method and kit for detection of analytes by production of electrochemical species |
| KR20180105198A (ko) * | 2016-01-27 | 2018-09-27 | 더 제너럴 하스피탈 코포레이션 | 자기적 전기화학적 감지 |
| ES2794173B2 (es) * | 2019-05-17 | 2022-07-21 | Univ Madrid Complutense | Método y plataforma electroquímica inmunosensora para la detección y/o cuantificación de adulteraciones en leche y productos lácteos |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4440487A1 (de) * | 1994-11-12 | 1996-05-15 | Behringwerke Ag | Heterogener Immunoassay mittels präzipitierbarer Festphase |
| ES2307430B1 (es) * | 2007-05-09 | 2009-10-20 | Consejo Superior De Investigaciones Cientificas | Biosensor y sus aplicaciones. |
| AU2012202981B2 (en) * | 2009-09-30 | 2014-01-23 | Cilag Gmbh International | Adhesive Composition for Use in an Immunosensor |
| WO2012016357A1 (en) * | 2010-08-06 | 2012-02-09 | Capitalbio Corporation | Microarray-based assay integrated with particles for analyzing molecular interactions |
| GB201218555D0 (en) * | 2012-10-16 | 2012-11-28 | Mode Diagnostics Ltd | Immunoassay using electrochemical detection |
-
2013
- 2013-03-20 ES ES201330398A patent/ES2498790B2/es not_active Expired - Fee Related
-
2014
- 2014-03-18 WO PCT/EP2014/055367 patent/WO2014147049A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| ES2498790A1 (es) | 2014-09-25 |
| WO2014147049A1 (en) | 2014-09-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
Ref document number: 2498790 Country of ref document: ES Kind code of ref document: B2 Effective date: 20150625 |
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| FD2A | Announcement of lapse in spain |
Effective date: 20210929 |