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GB2147100A - Multi-well plate inoculating means - Google Patents
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GB2147100A - Multi-well plate inoculating means - Google Patents

Multi-well plate inoculating means Download PDF

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Publication number
GB2147100A
GB2147100A GB08419819A GB8419819A GB2147100A GB 2147100 A GB2147100 A GB 2147100A GB 08419819 A GB08419819 A GB 08419819A GB 8419819 A GB8419819 A GB 8419819A GB 2147100 A GB2147100 A GB 2147100A
Authority
GB
United Kingdom
Prior art keywords
liquid
wells
well
well plate
support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB08419819A
Other versions
GB2147100B (en
GB8419819D0 (en
Inventor
John Ernest Foster Holley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ORBEC Ltd
Original Assignee
ORBEC Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ORBEC Ltd filed Critical ORBEC Ltd
Publication of GB8419819D0 publication Critical patent/GB8419819D0/en
Publication of GB2147100A publication Critical patent/GB2147100A/en
Application granted granted Critical
Publication of GB2147100B publication Critical patent/GB2147100B/en
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

A multi-well plate 6 has a channel connecting the wells in each row of wells. Liquid is introduced into a reservoir 4 at one end and the plate placed on support 2 at a pre-set angle to the horizontal. The liquid runs down the channels and fills the wells to a pre-determined level. <IMAGE>

Description

SPECIFICATION Innoculating means This invention relates to a method and apparatus for automaticaly innoculating multi-well plates.
Multi-well plates are known which are used for carrying out a plurality of microbiological reactions. For example in the testing the effectiveness of antibiotics against a liquid sample a multi-well plate can have its wells innoculated with the sample, and a series of antibiotics innoculated into the weis. The plate can then be incubated and growth or otherwise in each well noted.
Typical multi-well plates have an array of wells set in a plate. A typical multi-well plate has an array of 4x8 wells, although other configurations are used.
It is a time consuming business to innoculate al the wells in a multi-well plate individually and the present invention relates to a multi-well plate and equipment to be used therewith which makes innoculation easier and quicker.
According to the invention there is provided a means to innoculate wells in a multi-well plate, which means comprises a support which is adapted to support a multi-well plate and which support can be adjusted to be at a range of angles to the horizontal, an innoculating system which comprises a reservoir for liquid and a liquid dispenser adapted to dispense liquid in separate channels to correspond to rows of wells in a multi-well plate so that the dispenser can innoculate each row of wells in a multi-well plate placed on the support by placing liquid in the reservoir and tilting the support so that the reservoir is at the higher end.
Preferably the multi-well plate has wells formed in it in rows and the wells in each row are interconnected by depressions made in the walls separating the wells in each row. Thus the preferred plate allows liquid to flow from well to well in a row when the plate is inclined.
In a preferred form of the apparatus of the invention there is a liquid storage means incorporated in the apparatus and means for readily transferring the liquid to the reservoir and hence to the liquid dispenser. The reservoir and liquid dispenser can form part of the same structure.
The dispenser can comprise a plurality of nozzles connected by tubing to the liquid storage means so that the liquid can be pumped up to flow out of the nozzles. Preferably the dispenser also incorporates a plurality of baffles which form channels which cooperate with the wells in a multi-well plate placed on the support.
At the bottom of the apparatus of the invention (when the apparatus is in use) there is preferably a collector tray for the collected liquid.
As can be seen the maximum amount of liquid in any well in a multi-well plate which is placed at an angle to the horizontal will depend on the angle. If there is too much liquid it will overflow the edge of the well. Thus by adjusting the angle of a multi-well plate and dispensing excess liquid in the uppermost well the amount of liquid in each well can be predetermined. Preferably the apparatus is calibrated for a particular type of plate to give suitable alternative amounts of liquid in the wells.
The invention is further described with reference to the accompanying drawings in which Fig. 1 shows the apparatus in use and Fig. 2 shows a magnified view of a well.
The unit comprises a base 1 with support 2 adjustable to be at various angles. There is inside the base a liquid storage means and pump (not shown). Connected to the storage means and pump are nozzles 3. The nozzles discharge into reservoir 4. The reservoir 4 has baffles 5 defining channels.
When a plate 6 is placed on support 2 the channels line up with the rows of wells. In use the support 2 is placed at the predetermined angle and liquid pumped into reservoir 4. By means of grooves in plate 6 the liquid flows into all the wells.
The mechanism of flow is shown in Fig. 2.
Excess liquid flows into tray 7.
Thus the wells are quickly innoculated.
1. Equipment for innoculating wells in a multi-well plate which equipment comprises a support adapted to support a multi-well plate, which support can be adjusted to a range of angles to the horizontal and an innoculating system which comprises a reservoir for liquid and a liquid dispenser adapted to dispense liquid in separate channels, which channels correspond to rows of wells in a multi-well plate placed on the said support, the reservoir being positioned so that when the said support is placed at angle to the horizontal the reservoir is at the elevated end of the support.
2. Equipment as claimed in claim 1 in which there is a liquid storage means incorporated in the equipment and means for transferring the liquid from the liquid storage means to the reservoir and thence to the said liquid dispenser.
3. Equipment as claimed in claim 1 or 2 in which the dispenser comprises a plurality of nozzles connected by tubing to the liquid storage means and a plurality of baffles which define channels which channels cooperate with wells in a multi-well plate placed on the support.
4. Equipment as claimed in any one of claims 1-3 in which there is a tray for liquid
**WARNING** end of DESC field may overlap start of CLMS **.

Claims (6)

**WARNING** start of CLMS field may overlap end of DESC **. SPECIFICATION Innoculating means This invention relates to a method and apparatus for automaticaly innoculating multi-well plates. Multi-well plates are known which are used for carrying out a plurality of microbiological reactions. For example in the testing the effectiveness of antibiotics against a liquid sample a multi-well plate can have its wells innoculated with the sample, and a series of antibiotics innoculated into the weis. The plate can then be incubated and growth or otherwise in each well noted. Typical multi-well plates have an array of wells set in a plate. A typical multi-well plate has an array of 4x8 wells, although other configurations are used. It is a time consuming business to innoculate al the wells in a multi-well plate individually and the present invention relates to a multi-well plate and equipment to be used therewith which makes innoculation easier and quicker. According to the invention there is provided a means to innoculate wells in a multi-well plate, which means comprises a support which is adapted to support a multi-well plate and which support can be adjusted to be at a range of angles to the horizontal, an innoculating system which comprises a reservoir for liquid and a liquid dispenser adapted to dispense liquid in separate channels to correspond to rows of wells in a multi-well plate so that the dispenser can innoculate each row of wells in a multi-well plate placed on the support by placing liquid in the reservoir and tilting the support so that the reservoir is at the higher end. Preferably the multi-well plate has wells formed in it in rows and the wells in each row are interconnected by depressions made in the walls separating the wells in each row. Thus the preferred plate allows liquid to flow from well to well in a row when the plate is inclined. In a preferred form of the apparatus of the invention there is a liquid storage means incorporated in the apparatus and means for readily transferring the liquid to the reservoir and hence to the liquid dispenser. The reservoir and liquid dispenser can form part of the same structure. The dispenser can comprise a plurality of nozzles connected by tubing to the liquid storage means so that the liquid can be pumped up to flow out of the nozzles. Preferably the dispenser also incorporates a plurality of baffles which form channels which cooperate with the wells in a multi-well plate placed on the support. At the bottom of the apparatus of the invention (when the apparatus is in use) there is preferably a collector tray for the collected liquid. As can be seen the maximum amount of liquid in any well in a multi-well plate which is placed at an angle to the horizontal will depend on the angle. If there is too much liquid it will overflow the edge of the well. Thus by adjusting the angle of a multi-well plate and dispensing excess liquid in the uppermost well the amount of liquid in each well can be predetermined. Preferably the apparatus is calibrated for a particular type of plate to give suitable alternative amounts of liquid in the wells. The invention is further described with reference to the accompanying drawings in which Fig. 1 shows the apparatus in use and Fig. 2 shows a magnified view of a well. The unit comprises a base 1 with support 2 adjustable to be at various angles. There is inside the base a liquid storage means and pump (not shown). Connected to the storage means and pump are nozzles 3. The nozzles discharge into reservoir 4. The reservoir 4 has baffles 5 defining channels. When a plate 6 is placed on support 2 the channels line up with the rows of wells. In use the support 2 is placed at the predetermined angle and liquid pumped into reservoir 4. By means of grooves in plate 6 the liquid flows into all the wells. The mechanism of flow is shown in Fig. 2. Excess liquid flows into tray 7. Thus the wells are quickly innoculated. CLAIMS
1. Equipment for innoculating wells in a multi-well plate which equipment comprises a support adapted to support a multi-well plate, which support can be adjusted to a range of angles to the horizontal and an innoculating system which comprises a reservoir for liquid and a liquid dispenser adapted to dispense liquid in separate channels, which channels correspond to rows of wells in a multi-well plate placed on the said support, the reservoir being positioned so that when the said support is placed at angle to the horizontal the reservoir is at the elevated end of the support.
2. Equipment as claimed in claim 1 in which there is a liquid storage means incorporated in the equipment and means for transferring the liquid from the liquid storage means to the reservoir and thence to the said liquid dispenser.
3. Equipment as claimed in claim 1 or 2 in which the dispenser comprises a plurality of nozzles connected by tubing to the liquid storage means and a plurality of baffles which define channels which channels cooperate with wells in a multi-well plate placed on the support.
4. Equipment as claimed in any one of claims 1-3 in which there is a tray for liquid excess liquid dispensed by the liquid dispenser.
5. A process for innoculating wells in a multi-well plate which multi-well plate has wells formed in it in rows and the wells in each row are interconnected by depressions made in the walls separating the wells in each row, which process comprises placing the multi-well plate at an angle to the horizontal whereby each of the said rows of wells are at an angle to the horizontal and placing liquid in the wells at the elevated end of the said multi-well plate whereby liquid flows from well to well in each row of wells via the said depressions.
6. Equipment as herein described with reference to the drawing.
GB08419819A 1983-08-05 1984-08-03 Multiwell plate inoculating means Expired GB2147100B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB838321239A GB8321239D0 (en) 1983-08-05 1983-08-05 Innoculating means

Publications (3)

Publication Number Publication Date
GB8419819D0 GB8419819D0 (en) 1984-09-05
GB2147100A true GB2147100A (en) 1985-05-01
GB2147100B GB2147100B (en) 1987-01-07

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GB838321239A Pending GB8321239D0 (en) 1983-08-05 1983-08-05 Innoculating means
GB08419819A Expired GB2147100B (en) 1983-08-05 1984-08-03 Multiwell plate inoculating means

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Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7033747B2 (en) 2001-04-11 2006-04-25 Nagaoka & Co., Ltd Multi-parameter assays including analysis discs and methods relating thereto
US7387898B1 (en) 1996-10-08 2008-06-17 Burstein Technologies, Inc. Apparatus and method for conducting assays
US9573128B1 (en) * 2012-09-06 2017-02-21 SciKon Innovation, Inc. Fluidics device allowing fluid flow between a plurality of wells
US9829499B2 (en) 2015-03-23 2017-11-28 SciKon Innovation, Inc. Method and related systems for use with a fluidics device
US10035145B2 (en) 2014-12-02 2018-07-31 SciKon Innovation, Inc. Piston assembly and related systems for use with a fluidics device
WO2019014635A1 (en) * 2017-07-14 2019-01-17 Corning Incorporated 3d cell culture vessels for manual or automatic media exchange
JP2019505214A (en) * 2016-02-04 2019-02-28 マサチューセッツ インスティテュート オブ テクノロジー Modular organ microphysiological system with integrated pumping, leveling and sensing
JP2020526216A (en) * 2017-07-14 2020-08-31 コーニング インコーポレイテッド Cell culture tank with a porous support
WO2021026010A1 (en) 2019-08-02 2021-02-11 Molecular Devices, Llc Method and system for cultivating cells in media-exchanging wells
US11441121B2 (en) 2013-04-30 2022-09-13 Corning Incorporated Spheroid cell culture article and methods thereof
US11584906B2 (en) 2017-07-14 2023-02-21 Corning Incorporated Cell culture vessel for 3D culture and methods of culturing 3D cells
US11613722B2 (en) 2014-10-29 2023-03-28 Corning Incorporated Perfusion bioreactor platform
US11661574B2 (en) 2018-07-13 2023-05-30 Corning Incorporated Fluidic devices including microplates with interconnected wells
US11732227B2 (en) 2018-07-13 2023-08-22 Corning Incorporated Cell culture vessels with stabilizer devices
US11749437B2 (en) 2018-05-01 2023-09-05 Massachusetts Institute Of Technology Pumps and hardware for organ-on-chip platforms
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
US11912968B2 (en) 2018-07-13 2024-02-27 Corning Incorporated Microcavity dishes with sidewall including liquid medium delivery surface
US11976263B2 (en) 2014-10-29 2024-05-07 Corning Incorporated Cell culture insert
US12065635B2 (en) 2018-03-19 2024-08-20 Massachusetts Institute Of Technology Organ-on-chip platforms with reduced fluid volume
US12203059B2 (en) 2014-10-29 2025-01-21 Corning Incorporated Microwell design and fabrication for generation of cell culture aggregates

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US8894946B2 (en) * 2011-10-21 2014-11-25 Integenx Inc. Sample preparation, processing and analysis systems
CN104762198A (en) * 2015-04-16 2015-07-08 润科生物工程（福建）有限公司 Special inoculation frame for liquid cell culture

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1436184A (en) * 1972-08-18 1976-05-19 Pfizer Compartmented container
GB1572596A (en) * 1976-12-06 1980-07-30 Opto Electronic Displays Ltd Apparatus and method for innoculation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1436184A (en) * 1972-08-18 1976-05-19 Pfizer Compartmented container
GB1572596A (en) * 1976-12-06 1980-07-30 Opto Electronic Displays Ltd Apparatus and method for innoculation

Cited By (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7387898B1 (en) 1996-10-08 2008-06-17 Burstein Technologies, Inc. Apparatus and method for conducting assays
US7033747B2 (en) 2001-04-11 2006-04-25 Nagaoka & Co., Ltd Multi-parameter assays including analysis discs and methods relating thereto
US9573128B1 (en) * 2012-09-06 2017-02-21 SciKon Innovation, Inc. Fluidics device allowing fluid flow between a plurality of wells
US11441121B2 (en) 2013-04-30 2022-09-13 Corning Incorporated Spheroid cell culture article and methods thereof
US12146154B2 (en) 2013-04-30 2024-11-19 Corning Incorporated Spheroid cell culture article and methods thereof
US12203059B2 (en) 2014-10-29 2025-01-21 Corning Incorporated Microwell design and fabrication for generation of cell culture aggregates
US11976263B2 (en) 2014-10-29 2024-05-07 Corning Incorporated Cell culture insert
US11613722B2 (en) 2014-10-29 2023-03-28 Corning Incorporated Perfusion bioreactor platform
US10035145B2 (en) 2014-12-02 2018-07-31 SciKon Innovation, Inc. Piston assembly and related systems for use with a fluidics device
US9829499B2 (en) 2015-03-23 2017-11-28 SciKon Innovation, Inc. Method and related systems for use with a fluidics device
US11732229B2 (en) 2016-02-04 2023-08-22 Massachusetts Institute Of Technology Modular organ microphysiological system with integrated pumping, leveling, and sensing
US10876088B2 (en) * 2016-02-04 2020-12-29 Massachusetts Institute Of Technology Modular organ microphysiological system with integrated pumping, leveling, and sensing
JP2019505214A (en) * 2016-02-04 2019-02-28 マサチューセッツ インスティテュート オブ テクノロジー Modular organ microphysiological system with integrated pumping, leveling and sensing
JP2020527346A (en) * 2017-07-14 2020-09-10 コーニング インコーポレイテッド 3D cell culture vessel for manual or automatic medium replacement
US11970682B2 (en) 2017-07-14 2024-04-30 Corning Incorporated 3D cell culture vessels for manual or automatic media exchange
WO2019014635A1 (en) * 2017-07-14 2019-01-17 Corning Incorporated 3d cell culture vessels for manual or automatic media exchange
US11345880B2 (en) 2017-07-14 2022-05-31 Corning Incorporated 3D cell culture vessels for manual or automatic media exchange
JP2020526216A (en) * 2017-07-14 2020-08-31 コーニング インコーポレイテッド Cell culture tank with a porous support
US11584906B2 (en) 2017-07-14 2023-02-21 Corning Incorporated Cell culture vessel for 3D culture and methods of culturing 3D cells
US12311374B2 (en) 2017-07-14 2025-05-27 Corning Incorporated Cell culture vessel
US11767499B2 (en) 2017-07-14 2023-09-26 Corning Incorporated Cell culture vessel
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
US12065635B2 (en) 2018-03-19 2024-08-20 Massachusetts Institute Of Technology Organ-on-chip platforms with reduced fluid volume
US11749437B2 (en) 2018-05-01 2023-09-05 Massachusetts Institute Of Technology Pumps and hardware for organ-on-chip platforms
US11912968B2 (en) 2018-07-13 2024-02-27 Corning Incorporated Microcavity dishes with sidewall including liquid medium delivery surface
US11732227B2 (en) 2018-07-13 2023-08-22 Corning Incorporated Cell culture vessels with stabilizer devices
US12448594B2 (en) 2018-07-13 2025-10-21 Corning Incorporated Fluidic devices including microplates with interconnected wells
US11661574B2 (en) 2018-07-13 2023-05-30 Corning Incorporated Fluidic devices including microplates with interconnected wells
US12270017B2 (en) 2018-07-13 2025-04-08 Corning Incorporated Cell culture vessels with stabilizer devices
EP4007806A4 (en) * 2019-08-02 2023-08-23 Molecular Devices, LLC METHOD AND SYSTEM FOR CULTIVATION OF CELLS IN MEDIA EXCHANGE WELLS
WO2021026010A1 (en) 2019-08-02 2021-02-11 Molecular Devices, Llc Method and system for cultivating cells in media-exchanging wells
US12509652B2 (en) 2019-08-02 2025-12-30 Molecular Devices, Llc Method and system for cultivating cells in media-exchanging wells

Also Published As

Publication number Publication date
GB8321239D0 (en) 1983-09-07
GB2147100B (en) 1987-01-07
GB8419819D0 (en) 1984-09-05

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