GB2189146A - Virucidal composition comprising glutaraldehyde and a quaternary ammonium salt - Google Patents
Virucidal composition comprising glutaraldehyde and a quaternary ammonium salt Download PDFInfo
- Publication number
- GB2189146A GB2189146A GB08709209A GB8709209A GB2189146A GB 2189146 A GB2189146 A GB 2189146A GB 08709209 A GB08709209 A GB 08709209A GB 8709209 A GB8709209 A GB 8709209A GB 2189146 A GB2189146 A GB 2189146A
- Authority
- GB
- United Kingdom
- Prior art keywords
- glutaraldehyde
- composition
- arquad
- virucidal
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 43
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 title claims abstract description 23
- 230000003253 viricidal effect Effects 0.000 title claims abstract description 20
- 150000003242 quaternary ammonium salts Chemical class 0.000 title description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims abstract description 14
- 239000007864 aqueous solution Substances 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims description 4
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 208000008071 Parvoviridae Infections Diseases 0.000 claims 1
- 239000012895 dilution Substances 0.000 abstract description 8
- 238000010790 dilution Methods 0.000 abstract description 8
- 239000006172 buffering agent Substances 0.000 abstract description 4
- 239000012141 concentrate Substances 0.000 abstract description 4
- 208000036142 Viral infection Diseases 0.000 abstract 1
- 230000009385 viral infection Effects 0.000 abstract 1
- 241000700605 Viruses Species 0.000 description 10
- 206010018910 Haemolysis Diseases 0.000 description 9
- 230000008588 hemolysis Effects 0.000 description 9
- 239000004094 surface-active agent Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 239000003093 cationic surfactant Substances 0.000 description 4
- 239000000645 desinfectant Substances 0.000 description 3
- 239000002304 perfume Substances 0.000 description 3
- 241000125945 Protoparvovirus Species 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 230000003330 sporicidal effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- MPNXSZJPSVBLHP-UHFFFAOYSA-N 2-chloro-n-phenylpyridine-3-carboxamide Chemical compound ClC1=NC=CC=C1C(=O)NC1=CC=CC=C1 MPNXSZJPSVBLHP-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000046998 Canine minute virus Species 0.000 description 1
- 241000701931 Canine parvovirus Species 0.000 description 1
- 241001647378 Chlamydia psittaci Species 0.000 description 1
- 241000701925 Feline parvovirus Species 0.000 description 1
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 1
- 241000702619 Porcine parvovirus Species 0.000 description 1
- 241000701093 Suid alphaherpesvirus 1 Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000001049 brown dye Substances 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 150000003856 quaternary ammonium compounds Chemical class 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- HVLUSYMLLVVXGI-USGGBSEESA-M trimethyl-[(z)-octadec-9-enyl]azanium;chloride Chemical compound [Cl-].CCCCCCCC\C=C/CCCCCCCC[N+](C)(C)C HVLUSYMLLVVXGI-USGGBSEESA-M 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
A virucidal composition for use in preventing the spread of a range of viral infections includes glutaraldehyde and dimethylcocobenzylammonmium chloride. The composition can be used as an aqueous solution without the addition of a buffering agent and is normally supplied to the intended user in the form of a concentrate for dilution at the appropriate rate.
Description
SPECIFICATION
Virucidal composition
Field of the Invention
This invention relates to virucidal compositions and to methods of disinfecting using such compositions.
The invention is more specifically concerned with a virucidal composition which includes glutaraldehyde.
Description of the Prior Art
The use of sporicidal compositions containing glutaraldehyde and a cationic surfactant is disclosed in British Patent Specification number 1052537. In use, it has been discovered that these compositions have a virucidal as well as sporicidal activity.
A disadvantage of the particular compositions disclosed in Patent Specification number 1052537 is that aqueous solutions of the glutaraldehyde and surfactant need to be mixed with a buffering agent to enhance the activity of the composition.
Enveloped viruses which are surrounded by an inert sheath have been found to be treatable by such solutions. However, unenveloped viruses which have no inert sheath are much more difficult to treat. One such virus is the parvovirus, which has at least four strains, i.e. canine, feline, bovine and porcine parvoviruses.
It is an object of the invention to provide a virucidal composition which is effective against unenveloped viruses and which can be used without the addition of a buffering agent.
Summary of the Invention
According to one aspect of the present invention there is provided a virucidal composition comprising glutaraldehyde and dimethylcocobenzylammonium chloride.
According to another aspect of the invention there is provided a method of disinfecting an object which comprises subjecting the object to treatment with a virucidal composition comprising glutaraldehyde and dimethylcocobenzylammonium chloride.
The virucidal composition may be used as an aqueous solution which does not contain a buffering agent.
Description of the Preferred Embodiments
In one example of the invention a virucidal composition is prepared by introducing 200 litres of water into a mixing vessel equipped with a stirrer and adding thereto 30 grams of solubilised brown dye (18409). With continuous stirring 150 kg of glutaraldehyde solution are added followed by 100 kg. of cationic surfactant Arquad B50. 10 kg. of an aromatic perfume are then added and, when the mixture has been made up to 500 litres by the addition of further water, the mixture is then stirred for a further twenty minutes before batches of the composition are run off into suitable containers.
The above composition is an aqueous solution of the glutaraldehyde and surfactant and is hereinafter referred to as Composition A.
The Arquad B50 included in Composition A comprises an aqueous 50% by weight solution of quaternary ammonium salt the principal component of which is dimethylcocobenzylammonium chloride. The glutaraldehyde solution of Composition A is itself a 50% by weight solution of glutaraldehyde in water. Thus, Composition A comprises 15% by weight glutaraldehyde and
10% by weight cationic surfactant.
Tests have been carried out to establish the virucidal activity of Composition A in accordance with the standard test procedures of the Ministry of Agriculture, Fisheries and Food. These tests have shown the effectiveness of Composition A against the following viruses at the dilutions quoted, which give a 4 log10 reduction in virus titre:
Virus Dilution of Composition A
Parainfluenza 3 virus 1:750
Aujeszky's Disease Virus 1:1400
Paraviccima virus 1:1000
Transmissible gastroentiritis virus 1:900
Chlamydia psittaci 1:1000
Canine parvovirus 1:50
For use, therefore, as a spray in veterinary surgeries or as a disinfectant/detergent in premises where parvoviruses may be encountered, Composition A is used as a dilution in water of approximately 1:50 whereas, for general purposes, it can be used at a dilution of 1:500.At a dilution of 1:50, the concentration of glutaraldehyde is 0.3% by weight, the preferred concentra tion range being 0.2% to 1% by weight. At the same dilution the concentration of dimethylcocobenzylammonium chloride is 0.2%, the preferred concentration range being 0.1% to 0.6% by weight.
Similarly, at a dilution of 1:500, the concentration of glutaraldehyde is 0.03% and the concentration of dimethylcocobenzylammonium chloride is 0.02%
The preferred aromatic perfume for inclusion in the composition is that sold under the trade mark SOL-ODO-MASK H by Kilgern Chemicals Limited, of West Yorkshire.
Arquad B50 is produced by Akzo Chemie of Holland and chemical analysis of Arquad B50 shows that it comprises a number of structurally similar quaternary ammonium compounds and that the percentages by weight of the different carbon chain length compounds are as follows:
C10 C12 C14 C16 C18 C20 3 54 22 10 10
The term dimethylcocobenzylammonium chloride as used herein is thus to be interpreted as including a product one of the principai components of which has a 12 carbon chain.
Other cationic surfactants produced by Akzo Chemie include Arquad S50 (oleyltrimethylammonium chloride) and Arquad T50 (tallow trimethylammonium chloride).
The percentages by weight of the different carbon chain length constituents of Arquad S50 and Arquad T50 are as follows:
C10 C12 C14 C16 C18 C20
S50 - 1 4 12 82 1
T50 - 1 4 31 64 1
Tests have been carried out to compare the effectiveness of Composition A with that of
Compositions in which the Arquad B50 is replaced by Arquad S50 or Arquad T50. In these tests, a 5% aqueous solution of milk is exposed to disinfectant for varying lengths of time and the culture so obtained is plated on to a blood/agar plate which is then incubated at 37"C for 72 hours prior to examination.
The results which were obtained using 1% aqueous solutions of the disinfectants demonstrate the increased effectiveness of Composition A containing Arquad B50 and were as follows:
5 minutes 10 minutes 20 minutes 30 minutes
Arquad B50 No growth No growth No growth No growth
Arquad S50 Light Light Moderate Light
haemolysis haemolysis haemolysis haemolysis
Arquad T50 Light Moderate Severe Moderate haemolysis haemolysis haemolysis haemolysis haemolysis
On repetition of the 10 minutes exposure time tests to ascertain colony formation, no colonies were formed with Arquad B50, between 4 and 10 colonies were formed with Arquad S50, and between 11 and 50 colonies were formed with Arquad T50.
The 10 minute exposure tests were then repeated with two further surfactants produced by
Akzo Chemie. These were Arquad 2-C75, which is dicocodimethylammonium chloride, and
Ethoquad C-25, which is polyoxyethylenecocomethylammonium chloride. The percentages by weight of the different carbon chain length constitutents of Arquad 2-C75 and Ethoquad C-25 are:
C8 ClO C72 C14 C16 C18 2-C75 3 5 50 19 10 10
C-25 3 5 50 19 10 10
With Arquad B50 no colony growth occurred whereas with Arquad 2-C75 there was significant colony growth at two sites and with Ethoquad C-25 there was rapid colonial growth at one site.
These test results clearly demonstrate the surprising increased effectiveness of dimethylcocobenzylammonium chloride as compared to other surfactants of similar structural formulae.
Composition A contains an aqueous solution of glutaraldehyde and surfactant. As an alternative -thereto, the glutaraldehyde, surfactant, dye and perfume may be mixed together directly without the addition of water to form a concentrate. This concentrate is storage-stable and can be produced particularly for export purposes.
Composition A, or the concentrate when dissolved in an equal weight of water, has a pH in the region of 7.5. The glutaraldehyde and surfactant interact synergistically at this pH to obtain a virucidal activity which is significantly greater than either glutaraldehyde or the surfactant sepa rately.
Claims (7)
1. A virucidal composition comprising glutaraldehyde and dimethylcocobenzylammonium chloride.
2. A virucidal composition according to Claim 1, wherein the glutaraldehyde comprises a 50% by weight aqueous solution.
3. A virucidal composition according to claim 1, in the form of an aqueous solution containing 15% by weight glutaraldehyde and 10% by weight dimethylcocobenzylammonium chloride.
4. A virucidal composition according to claim 1, in the form of an aqueous solution containing 0.2 to 1% by weight glutaraldehyde and 0.1 to 0.6% by weight dimethylcocobenzylammonium chloride.
5. A virucidal composition for use in combatting parvovirus infections comprising an aqueous solution of Composition A as hereinbefore described.
6. A method of disinfecting an object which comprises subjecting the object to treatment with a virucidal composition comprising glutaraldehyde and dimethylcocobenzylammonium chloride.
7. A method according to Claim 6, wherein the virucidal composition comprises an aqueous solution of Composition A as hereinbefore described.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB868609329A GB8609329D0 (en) | 1986-04-16 | 1986-04-16 | Virucidal sporicidal composition |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB8709209D0 GB8709209D0 (en) | 1987-05-20 |
| GB2189146A true GB2189146A (en) | 1987-10-21 |
| GB2189146B GB2189146B (en) | 1989-04-05 |
Family
ID=10596323
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB868609329A Pending GB8609329D0 (en) | 1986-04-16 | 1986-04-16 | Virucidal sporicidal composition |
| GB8709209A Expired GB2189146B (en) | 1986-04-16 | 1987-04-16 | Virucidal composition comprising glutaraldehyde and a quaternary ammonium salt |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB868609329A Pending GB8609329D0 (en) | 1986-04-16 | 1986-04-16 | Virucidal sporicidal composition |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0275262A1 (en) |
| JP (1) | JPS63502990A (en) |
| AU (1) | AU589005B2 (en) |
| FI (1) | FI875510L (en) |
| GB (2) | GB8609329D0 (en) |
| WO (1) | WO1987006099A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0692192A1 (en) * | 1994-07-11 | 1996-01-17 | MFH Marienfelde GmbH Unternehmen für Hygiene | Alcoholic disinfecting agent preparation and use thereof |
| US8609381B2 (en) | 2007-06-28 | 2013-12-17 | Dow Brasil Sudeste Industrial Ltda. | Methods to control bacterial growth in fermentation processes |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3636541A1 (en) * | 1986-10-27 | 1988-04-28 | Henkel Kgaa | DISINFECTANT WITH PARASITE KILLING EFFECT |
| US5674829A (en) * | 1993-01-29 | 1997-10-07 | Antoinetta P. Martin | Stable aqueous glutaraldehyde solutions containing sodium acetate and a nonionic detergent |
| DE102006009508A1 (en) * | 2006-02-27 | 2007-08-30 | Gerhard Ruff Gmbh | Disinfectant containing glutaric dialdehyde and quaternary ammonium compounds |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1499789A (en) * | 1975-06-16 | 1978-02-01 | Henkel & Cie Gmbh | Disinfectants |
| GB2074866A (en) * | 1980-04-25 | 1981-11-11 | Anios Lab Sarl | Bactericidal Compositions |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1052537A (en) * | 1963-05-10 | |||
| DE3439519A1 (en) * | 1984-10-29 | 1986-04-30 | Henkel KGaA, 4000 Düsseldorf | METHOD FOR IMPROVING THE CORROSION BEHAVIOR OF DISINFECTANT SOLUTIONS |
-
1986
- 1986-04-16 GB GB868609329A patent/GB8609329D0/en active Pending
-
1987
- 1987-04-16 GB GB8709209A patent/GB2189146B/en not_active Expired
- 1987-04-16 WO PCT/GB1987/000256 patent/WO1987006099A1/en not_active Ceased
- 1987-04-16 AU AU72878/87A patent/AU589005B2/en not_active Ceased
- 1987-04-16 FI FI875510A patent/FI875510L/en not_active IP Right Cessation
- 1987-04-16 EP EP87902611A patent/EP0275262A1/en not_active Withdrawn
- 1987-04-16 JP JP62502444A patent/JPS63502990A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1499789A (en) * | 1975-06-16 | 1978-02-01 | Henkel & Cie Gmbh | Disinfectants |
| GB2074866A (en) * | 1980-04-25 | 1981-11-11 | Anios Lab Sarl | Bactericidal Compositions |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0692192A1 (en) * | 1994-07-11 | 1996-01-17 | MFH Marienfelde GmbH Unternehmen für Hygiene | Alcoholic disinfecting agent preparation and use thereof |
| US8609381B2 (en) | 2007-06-28 | 2013-12-17 | Dow Brasil Sudeste Industrial Ltda. | Methods to control bacterial growth in fermentation processes |
Also Published As
| Publication number | Publication date |
|---|---|
| GB8709209D0 (en) | 1987-05-20 |
| GB2189146B (en) | 1989-04-05 |
| EP0275262A1 (en) | 1988-07-27 |
| FI875510A7 (en) | 1987-12-15 |
| WO1987006099A1 (en) | 1987-10-22 |
| FI875510A0 (en) | 1987-12-15 |
| AU7287887A (en) | 1987-11-09 |
| JPS63502990A (en) | 1988-11-02 |
| FI875510L (en) | 1987-12-15 |
| GB8609329D0 (en) | 1986-05-21 |
| AU589005B2 (en) | 1989-09-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 737B | Application made to the patents court (sect. 37(1)/1977) | ||
| 737A | Proceeding under section 37(1) patents act 1977 | ||
| PCNP | Patent ceased through non-payment of renewal fee | ||
| 728C | Application made for restoration (sect. 28/1977) | ||
| 728A | Order made restoring the patent (sect. 28/1977) | ||
| 732E | Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977) | ||
| 772 | Application made for revocation (sect. 72/1977) | ||
| 732E | Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977) | ||
| 772C | Application made to the comptroller for revocation (sect. 72/1977) | ||
| 772S | Application withdrawn ** no order for revocation made (sect. 72/1977) | ||
| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19960416 |