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GB2248235A - Quinoline-sulphonamides having smooth muscle relaxation activity - Google Patents
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GB2248235A - Quinoline-sulphonamides having smooth muscle relaxation activity - Google Patents

Quinoline-sulphonamides having smooth muscle relaxation activity Download PDF

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GB2248235A
GB2248235A GB9122595A GB9122595A GB2248235A GB 2248235 A GB2248235 A GB 2248235A GB 9122595 A GB9122595 A GB 9122595A GB 9122595 A GB9122595 A GB 9122595A GB 2248235 A GB2248235 A GB 2248235A
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Hiroyoshi Hidaka
Tomohiko Ishikawa
Masatoshi Hagiwara
Tsutomu Inou
Kenji Naito
Osamu Sakuma
Masayuki Yuasa
Tadashi Morita
Isao Umezawa
Takashi Inaba
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Tobishi Pharmaceutical Co Ltd
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Tobishi Pharmaceutical Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
    • C07D295/16—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms acylated on ring nitrogen atoms
    • C07D295/18—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms acylated on ring nitrogen atoms by radicals derived from carboxylic acids, or sulfur or nitrogen analogues thereof
    • C07D295/182—Radicals derived from carboxylic acids
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    • C07C211/00—Compounds containing amino groups bound to a carbon skeleton
    • C07C211/43—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton
    • C07C211/44—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton having amino groups bound to only one six-membered aromatic ring
    • C07C211/49—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton having amino groups bound to only one six-membered aromatic ring having at least two amino groups bound to the carbon skeleton
    • C07C211/50—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton having amino groups bound to only one six-membered aromatic ring having at least two amino groups bound to the carbon skeleton with at least two amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton
    • C07C211/51—Phenylenediamines
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    • C07C229/52—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to carbon atoms of six-membered aromatic rings of the same carbon skeleton
    • C07C229/54—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to carbon atoms of six-membered aromatic rings of the same carbon skeleton with amino and carboxyl groups bound to carbon atoms of the same non-condensed six-membered aromatic ring
    • C07C229/60—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to carbon atoms of six-membered aromatic rings of the same carbon skeleton with amino and carboxyl groups bound to carbon atoms of the same non-condensed six-membered aromatic ring with amino and carboxyl groups bound in meta- or para- positions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C271/00—Derivatives of carbamic acids, i.e. compounds containing any of the groups, the nitrogen atom not being part of nitro or nitroso groups
    • C07C271/06—Esters of carbamic acids
    • C07C271/08—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
    • C07C271/10—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C271/16—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms to carbon atoms of hydrocarbon radicals substituted by singly-bound oxygen atoms
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    • C07C271/00—Derivatives of carbamic acids, i.e. compounds containing any of the groups, the nitrogen atom not being part of nitro or nitroso groups
    • C07C271/06—Esters of carbamic acids
    • C07C271/08—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
    • C07C271/10—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C271/22—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms to carbon atoms of hydrocarbon radicals substituted by carboxyl groups
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    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C57/00—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms
    • C07C57/64—Acyl halides
    • C07C57/76—Acyl halides containing halogen outside the carbonyl halide groups
    • C—CHEMISTRY; METALLURGY
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D217/00—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems
    • C07D217/02—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems with only hydrogen atoms or radicals containing only carbon and hydrogen atoms, directly attached to carbon atoms of the nitrogen-containing ring; Alkylene-bis-isoquinolines
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    • C07D295/00—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms
    • C07D295/04—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms
    • C07D295/12—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly or doubly bound nitrogen atoms
    • C07D295/125—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms with substituted hydrocarbon radicals attached to ring nitrogen atoms substituted by singly or doubly bound nitrogen atoms with the ring nitrogen atoms and the substituent nitrogen atoms attached to the same carbon chain, which is not interrupted by carbocyclic rings
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    • C07D295/16—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms acylated on ring nitrogen atoms
    • C07D295/20—Heterocyclic compounds containing polymethylene-imine rings with at least five ring members, 3-azabicyclo [3.2.2] nonane, piperazine, morpholine or thiomorpholine rings, having only hydrogen atoms directly attached to the ring carbon atoms acylated on ring nitrogen atoms by radicals derived from carbonic acid, or sulfur or nitrogen analogues thereof
    • C07D295/205—Radicals derived from carbonic acid
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
    • C07D409/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings
    • C07D409/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
    • C07D491/10—Spiro-condensed systems

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Abstract

The present invention provides a compound represented by the formula (I): <IMAGE> wherein Y represents N, or CH; R, represents hydrogen, optionally substituted alkyl, formyl, halophenylpropargyl, optionally substituted aralkyl or optionally substituted phenyl, and R2 represents a group represented by the formula (II); <IMAGE> wherein R3 represents hydrogen, optionally substituted alkyl, formyl, halophenylpropargyl, optionally substituted aralkyl or optionally substituted phenyl: or R1 and R3 together form an alkylene group; R4 represents hydrogen or alkyl; R5 represents hydrogen, halogen, nitro, alkyl, optionally substituted hydroxyl, optionally substituted amino, optionally substituted carboxy, polyfluoro-lower alkyl, cyano, hydroxymethyl, methylthio, methyl sulfinyl or methylsulfonyl; R6 represents hydrogen, halogen or alkoxy; or R5 and R6 together form an alkylenedioxy group: R7 represents hydrogen or alkoxy: X represents vinylene or ethynlene: Ar represents phenyl, naphthyl or a heterocyclyl group: m is from 1 to 3; and W represents alkylene, optionally substituted phenylene or optionally substituted phenylene-lower alkylene. Quinoline sulfonamino derivatives (I) have a vessel smooth muscle relaxation activity as well as a platelet agglutination inhibitory activity and inhibitory activity to protein kinase, proteinkinase C, and calmodulindependent proteinkinase II, but having little action on cardio function.

Description

COMPOUND HAVING VESSEL SMOOTH MUSCLE RELAXATION ACTIVITY The present invention relates to compounds having smooth muscle relaxation activity,and to their production and use Quinoline compounds having a vessel smooth muscle relaxation activity are described in, for example, Japanese Unexamined Patent Publication (KOKAI) Nos. 60-81168, 61-126026, 61-271221, 61-293914, 62-103066, and 63-211267; and U.S. Patent Nos. 4456757, 4525589, 4560755, 4634770, 4678783, 4709032, and 4798897.
Among the compounds described in the above references, some have a satisfactory smooth muscle relaxation activity, but have problems with relation to toxicity, organ-specificity, and safety.
The present invention provides novel compounds which Shave smooth muscle relaxation activity, and which can also have organ-specificity, safety, and low tonicity.
The- present invention provides a compound represented by the formula (I):
wherein Y represents N, H3C-N or CH; R1 represents a hydrogen atom, an optionally substituted lower alkyl group, a formyl group, a halophenylpropargyl group, an optionally substituted aralkyl group or optionally substituted phenyl; and (1) R2 represents a group represented by the formula (II):
wherein R3 represents a hydrogen atom, an optionally substituted lower alkyl group, a formyl group, a halophenylpropargyl group, an optionally substituted aralkyl group or optionally substituted phenyl; or R1 and R3 together form a lower alkylene group; R4 represents a hydrogen atom or a lower alkyl group; R5 represents a hydrogen atom, a halogen atom, a nitro group, a lower alkyl group, an optionally substitute hydroxyl group, an optionally substituted N-substituted amino group, an optionally substituted carboxy group, a polyfluoro-lower alkyl group, a cyano group, a hydroxymethyl group, a methylthio group, methyl sulfinyl group or methylsulfonyl group; R6 represents a hydrogen atom, a halogen atom or a lower alkoxy group; or R5 and R6 together form a lower alkylenedioxy group; R7 represents a hydrogen atom or a lower alkoxy group; X represents a vinylene group or an ethynylene group; Ar represents a phenyl group, a naphthyl group or a heterocyclyl group; m represents an integer of 1 to 3; and W represents a lower alkylene group, an optionally substituted phenylene group or an optionally substituted phenylene-lower alkylene group; or (2) R2 represents a group represented by the formula (III): wherein
R10 represents a hydrogen atom, a nitro group, an optionally substituted amino group, an optionally substituted hydroxyl group, a lower alkyl group, or a halogen atom; or R1 and R10 together form a lower alkylene group; R11 represents a hydrogen atom, a hydroxyl group or a lower alkoxy group; R12 and R13 each represent a hydrogen atom, or together represent = 0; Ar has the same meaning as defined under the formula (II); n represents an integer of 1 to 3; and A represents the group > CR14R15 or > NO14; w wherein R14 represents a hydrogen atom, an optionally substituted hydroxy group, an optionally substituted phenyl group, an acyl group, a substituted carbonyl group, an optionally substituted alkoxycarbonyl, a substituted carbamoyl, an optionally substituted amino group, an arylsulfonyl group, an aralkylsulfonyl group, an aralkyl group, or a heterocyclyl group; and R15 represents a hydrogen atom or a lower alkoxy group, or R15 and R14 together represent an alkylenedioxy group or = 0; and quaternary ammonium salts of the compound of the formula (I), and nontoxic salts of the compound of the formula (I).
The present invention also provides a process for the production of a compound represented by the formula (I) wherein R2 represents a group represented by the formula (II), comprising the steps of (1) reacting a compound represented by the formula (IV):
wherein Y, W, R1 and R3 have the same meanings as defined above, with a compound represented by the formula (V):
wherein B represents -CH2Hal or -CO-R4 , and other symbols have the same meanings as defined in claim 1; and optionally (2) reducing the compound produced in step (1), and/or optionally (3) alkylating or formylating the compound produced in step (1) or The present invention moreover provides a process for the production of a compound represented by the formula (I) wherein R2 represents a group represented by the formula (II), comprising the steps of: (1) reacting a compound represented by the formula (VI):
with a compound represented by the formula (VII):
or a reactive derivative thereof or a salt thereof, wherein all the symbols in formulae (VI) and (VII) have the same meanings as defined above, and optionally, (2) alkylating the compound produced in step (1).
The present invention still further provides a process for the production of a compound represented by the formula (I), according to claim 1, wherein R2 represents a group represented by the formula (III), comprising the steps of: (1) reacting a compound represented by the formula (VIII).
with a compound represented by the formula (VII):
or a reactive derivative thereof or a salt thereof, wherein all the symbols in the formulae (VII) and (VIII) have the some meanings as defined above; and optionally carrying out one or more than one of the following steps (2) to (8), (2) hydrolysis to form a free hydroxyl group or an amino group; (3) deprotection of a protecting group for a hydroxyl or amino group; (4) acylation or substituted-alkoxycarbonylation of a hydroxyl group or an amino group; (5) alkylation of a hydroxyl group or an amino group; (6) amination or hydroxylation of a carbonyl group; (7) reduction of a nitro group to an amino group; and (8) carbonylation of an acetal.
The present invention also provides a pharmaceutical composition comprising a compound described above.
DESCRIPTION OF THE PREFERRED EMBODIMENTS In the definition of the present invention, the optionally substituted lower alkyl includes an unsubstituted lower alkyl and a substituted lower alkyl.
"Lower alkyl" means an alkyl containing up to seven carbon atoms, preferably up to four carbon atoms. The unsubstituted lower alkylsinclude straight chain lower alkyl and branched chain lower alkyl and are, for example, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, pentyl, hexyl and heptyl.
In the definition of R1 and R3 , the substituted lower alkyls include an optionally substituted amino lower alkyl, such as 2-amino ethyl and 3-amino propyl, N,N-dimethyl amino propyl, 4-piperidyl lower alkyl such as 4-piperidyl propyl, a morpholino lower alkyl.such as morpholinoethyl, and piperidino lower alkyl such as piperidinoethyl. The halophenylpropargyl includes fluoro- chloro-, brom- and iodo-phenylpropagyls, and is preferably p-chlorophenylpropargyl. The optionally substituted aralkyls include unsubstituted aralkyl, for example, a phenyl lower alkyl such as benzyl and phenylethyl, and a substituted phenyl-lower alkyl such as p-methoxybenzyl. The optionally substituted phenyl includes a substituted phenyl such as 3,4-dimethoxy phenyl. The alkylene group formed by R1 and R3 is, for example, a methylene, ethylene, or propylene group.
The lower alkyl as R4 is as defined above.
The halogen as R5 is fluoro chloro, bromo or iodo, preferably chloro.
The lower alkyl as R5 is as defined above.
The optionally substituted hydroxy as R5 includes a hydroxyl group, and a substituted hydroxyl group, for example, a lower alkoxy such as methoxy, ethoxy or propoxy.
The optionally N-substituted amino as R5 is an amino, or a lower alkyl amino such as dimethylamino.
The optionally substituted carboxy as R5 includes a carboxyl group, per se., and a substituted carboxy, for example, a lower alkoxy carbonyl such as methoxy carbonyl, ethoxycarbonyl, and propoxycarbonyl.
The polyfluoro-lower alkyl as R5 is, for example, trifluoromethyl.
The halogen as R6 is as defined above for R5.
The lower alkoxy as R6 is as defined above for R5.
The lower alkylenedioxy formed by R5 and R6 is, for example, methylenedioxy, 1,2-ethylenedioxy, 1,3-dipropylenedioxy, 1,2-dipropylenedioxy, or the like, preferably 1,2-ethylenedioxy.
The lower alkoxy as R7 is as defined above for R5.
The lower alkylene group as W is, for example, a methylene group, ethylene group, 1,3-propylene group, or 1,4-butylene group.
The phenylene group as W is, for example, a 1,2-phenylene group or 1,3-phenylene. The phenylene lower alkylene group as W is, for example, a 1,2phenylene- or 1,3-phenylene-lower alkylene group such as 1,2-phenylene-ethylene or a 1,3-phenylene-ethylene group. The optional substituent for the phenylene moiety is, for example, a lower alkoxy carbonyl such as methoxy carbonyl.
The heterocyclyl group as Ar is, for example, a pyridyl such as 2-pyridyl, 3-pyridyl or 4-pyridyl, a pyridyl such as 2 pyridyl, 3-pyridyl or 4-pyridyl, a pyrrolyl such as 2-pyrrolyl or 3-pyrrolyl, a thionyl such as 2-thionyl a 3-thionyl, or a furyl such as 2-furyl or 3-furyl.
The optionally substituted amino as Rlo includes free amino and substituted amino. In the substituted amino, the substituents are exemplified by a lower alkyl such as methyl, ethyl, propyl or other lower alkyl as defined above, and substituted sulfo such as isoquinoline sulfo, naphtharenesulfo, methanesulfo, toluenesulfo. Accordingly, the substituted amino is, for example, isoquinoline sulfonamide, N-lower alkyl isoquinolinesulfonamide such as N-methyl-sulfonamide, naphtharenesulfonamide, N-lower alkyl naphtharenesulfonamide such as N-methylnaphtharenesulfonamide, methansulfonamide, N-lower alkyl methansulfonamide, N-methyl methansulfonamide, toluenesulfonamide, or N-lower alkyl methansulfonamide such as N-methyl methanesulfonamide. The substituted amino further is phtharimide.
The substituted hydroxy group as R10 includes ester, ether and protected hydroxy. The ester is, for example, a substituted sulfonyloxy such as isoquinolinesulfonyloxy, toluenesulfonyloxy or naphtharenesulfonyloxy, or a lower alkanoyloxy such as acetoxy, propionyloxy or butanoyloxy. The ether is, for example, a lower alkoxy such as methoxy, ethoxy or propoxy; an aralkyloxy such as benzyloxy; a lower alkanoyloxy-lower alkoxy such as acetoxy methoxy; or a heterocycle-lower alkoxy such as 2-pyridyl methoxy or 4-pyridylmethoxy.
The lower alkyl as R10 is as defined above. The halogen as R10 is as defined above. The lower alkoxy as R10 is as defined above for R5. The halogen as R10 is as defined above for R5. The heterocycle group as Ar2 is, for example1 an imidazolyl such as 4-imidazolyl.
The substituted hydroxyl group as R14 is, for example, an ether group such as a lower alkoxy defined as above, or an optionally substituted aralkyl, for example, phenyl-lower alkyl optionally substituted on the phenyl, such as benzyl, phenylpropyl, 4-methylbenzyl, 3,4-dichlorobenzyl, or an ester group, for example, a lower alkanoyloxy such as acetoxy or propanoyloxy.
The substituted phenyl as R14 is, for example, a lower alkylphenyl for example 3-methylpheny, a lower alkoxyphenyl such as 2,3- or a 4-methoxypheny, mono- or di-holophenyl such as 4-chlorophenyl, 3,4-dichlorophenyl.
The acyl as R14 is, for example, an acylphenyl such as benzoyl, on an aralkylcarbonyl such as benzylcarbonyl or phenylpropylcarbony.
The substituted alkoxycarbonyl R14 is, for example, a phenyl-lower alkoxycarbonyl such as benzyloxycarbonyl, or tert-butoxy carbonyl.
The substituted carbonyl is, for example, an arylcarbonyl such as phenylcarbonyl, or an aralkyl carbonyl such as benzylcarbonyl.
The substituted amino R14 is, for example, a lower alkylamino, an optionally substituted aralkylamine or N,N-lower alkyl aralkylamino, for example, methylamino benzylamino, 3,4-dichlorobenzylamino, N,N-methyl benzylamino or N,N-methyl 3,4-dichloroamino.
The aryl sulfonyl R14 is, for example, benzylsulfonyl, a isoquinolinesulfonyl.
The aralkylsulfonyl R14 is, for example, benzylsulfonyl or phenyl propylsulfonyl.
The aralkyl R14 is, for example, benzyl or phenylpropionyl.
The heterocyclyl group R14 is, for example, a pyridyl such as 2-pyridyl, or a pyrimidyl such as 2-pyrimidyl.
Since the present compounds have a nitrogen atom they can form quaternary ammonium salts, or salts such as nontoxic salts. To form a quaternary ammonium salt, a compound of the present invention is reacted with, for example, methyl iodide. The salts of the present invention are preferably nontoxicic salts, for example, salts with an inorganic acid such as hydrochloric acid, sulfuric acid, nitric acid, phosphorus acid, hydrogen bromide, hydrogen iodide or the like, as well as salts with an organic acid, such as citric acid, acetic acid, oxalic acid, tartaric acid, sulfonic acids such as methane sulfonic acid, ethanesulfonic acid benzenesulfonic acid, fumaric acid, maleic acid, malic acid or the like.
In an embodiment for the production of the present compounds, a compound represented by the formula (IV) as described above is reacted with a compound represented by the formula (V) as described above.
In a preferable embodiment of this variation, a compound of the formula (IV), wherein R1 and R3 represent a hydrogen atom, is reacted, and after the reaction, the resulting intermediate is derivatized, for example, alkylated or formulated to introduce R1 and/or R3.
In a particular case, an isoquinolinesulfonamide represented by the formula (IV'):
is reacted with a compound of the formula (V), and if necessary, the resulting intermediate is reduced. The reaction is carried out, for example, in a medium such as methanol, dimethylformamide, tetrahydrofuran, dimethy sulfoxide, diglyme, benzene, at a temperature of OOC to 100 C, preferably a room temperature. The reduction is carried out using, for example, sodium borohydride, aluminum lithium hydride or the like, at a temperature of OOC to 600C, preferably at a room temperature. The introduction of R1 and/or R3 can be carried out by using a halide of R1 and/or R3 , i.e., Hal-R1 or Hal-R2 while removing hydrogen halide. Where an alkylene halide is used, a compound wherein R1 and R3 are linked is provided. For an introduction of formyl, the intermediate is reacted with formic acid in the presence of acetic anhidride. The above-mentioned reactions are carried out, for example, in chloroform, dimethylacetamide, dimethylformamide or other aprotic solvent, at a temperature of about OOC to 1000C, preferably at a room temperature.
In another embodiment for the production of the present compounds, a compound of the formula (VI) is reacted with a compound (VII). In a preferable embodiment, a compound of the formula.(VI) wherein R3 is hydrogen atom is reacted with a compound of the formula (VII), and after the reaction, the resulting intermediate is alkylated to introduce R3.
In a particular embodiment, a compound of the formula (VI')
wherein R16 is an optional substituent on the phenyl moiety W, is reacted with a compound of the formula (VII) to obtain a compound represented by the formula (I-a):
The reaction is carried out in a medium such as pyridine, dimethylformamide, acetonitrile, dioxane, tetrahydrofuran, dichloromethane, chloroform or the like, at a temperature of about OOC to 400C, preferably 200C to 300C.
Note, the product (Ia) is reacted with a compound which introduces the substituent R1 and/or R3. The compound which introduces R1 and/or R3 is, for example, a halogen compound of R1 or R3 , i.e., Hal-R1 or Hal-R3 wherein Hal represents a halogen atom.
The reaction is carried out in a medium such as tetrahydrofuran, dimethylformamide, dioxane, deethoxymethane, methanol, ether such as ethyl ether, chloroform, ethyl acetate or the like in the pressure of a base which kinds the resulting hydrogen halide during the reaction, for example a tertiary amine such as pyridine, dimethylaminopyridine. N-methylpiperidine or triethylamine, or an inorganic base such as potassium bicarbonate, potassium hydroxide, sodium carbonate, sodium hydroxide or the like.
The starting material (VI) wherein R3 is a hydrogen atom can be obtained by reacting a compound represented by the formula (VIII):
with a compound represented by the formula (IX): H2N-W-NH2 (IX).
For example, to obtain the intermediate (VI'), a compound of the formula (VIII'):
with a compound of the formula (IX'):
These reactions can be carried out under substantially the same condition as for the introduction of R1 and R3.
In another embodiment for the production of the present compound (I), a compound of the formula (VIII) is reacted with a compound of the formula (VII).
The starting material (VIII) can be obtained by reacting a compound represented by the formula (X):
wherein R17 is a hydrogen atom or a lower alkyl, with a compound represented by the formula (XI):
By this reaction, the compound (VIII) wherein R12 and R13 together form =0 is obtained. A reduction of this compound provides the compound (VIII) wherein both R12 and R13 represent a hydrogen atom.
In a particular embodiment, a known compound tyrosine having the amino group protected, and represented by the formula (Xa):
is reacted with pyperazine having the nitrogen atom protected is represented by the formula (XIa):
to obtain an intermediate represented by the formula (VIIIa):
Next, the intermediate (VIIIa) is then condensed with isoquinolinesulfonylchloride to obtain a compound represented by the formula (XII):
Next, the following modification of the compound (XII) can be carried out to obtain some of the present compounds:: (2a) Hydrolysis to remove the isoquinolinesulfonyl group to free hydroxy on the phenyl ring; (3a) Deprotection of the piperazine ring; (4a) Acylation or alkylation of the free hydroxy; (4a) Acylation of the nitrogen atom of the piperazine moiety; (5a) Alkylation of the sulfonamide group.
To prepare other compounds of the present invention, bistidine, phenylalanine or-the like can be used in place of tyrosine, and/or piperidine or the like can be used in place of piperazine. Moreover, an N-alkylated compound can be used in place of an N-protected compound (IIa), and/or a hydroxy-protected compound of the compound (IIa) can be used. The compound (VIIIa) or (XII) can be reduced to convert the carbonyl structure to the methylene chain. The orth portion of the phenyl ring can be linked with an amino group via an alkylene chain to form a ring structure.
Where piperidine is used in place of piperazine, the piperidine moiety can be converted to a corresponding moiety having an acetal structure at the fourth position thereof. After condensation with a sulfonic acid derivative, (8a) the acetal can be carbonylated, (6a) the carbonyl can be converted to hydroxy or an amino group, (4b) the hydroxyl or amino group can be acylated, or (5b) the hydroxyl or amino group can be alkylated, to obtain some of the desired compounds of the present invention.
The reaction of the compound (X) and (XI) is carried out in a medium such as.tetrahydrofuran, dioxane, dichloromethane, or other aprotic solvent at a temperature of about OOC to 400C, preferably 200C to 300C.
The reaction of the compound (VII) and the compound (VIII) is carried out in an aprotic solvent such as tetrahydrofuran, methylene chloride, chloroform, dimethylformamide, or the like in the presence of a hose such as triethylamine or the like at a temperature of about OOC to 400C, preferably 200C to 300C.
The hydrolysis of step (2) is carried out in a solvent such as methanol, tetrahydrofuran, a mixture thereof, dimethyl-sulfoxide or the like, in the presence of a base such as sodium hydroxide, potassium hydroxide or the like.
The reprotection of step (3) is carried out in a solvent such as methanol, ethanol, chloroform, ethyl acetate or the like.
The acylation of step (4) is carried out in a solvent such as chloroform, tetrahydrofuran, pyridine or the like, in the presence of a base such as triethylamine.
The alkylation of step (5) is carried out in a solvent such as dimethylformamide, tetrahydrofuran, ethyl acetate, methanol, methylene chloride, or a mixture thereof.
The hydroxylation of step (6) is carried out in a protonic solvent such as methanol or ethanol in the presence of a reducing agent such as sodium borohydride, sodium cyanoborohydride. The amination of step (6) is carried out, after an imine formation, under the same condition as for the hydroxylation. The reduction of nitro in step (7) is carried out in a solvent, for example, an alcohol such as methanol or ethanol, by catalytic hydrogenation using as a catalyst a noble methanol catalyst such as palladium on carbon.
The conversion of acetol to oxo is carried out by acidic hydrolysis in an aqueous solution.
Examples The present invention will now be further illustrated by, but is by no means limited to, the following example.
In the Examples, melting points were determined by a melting point measurement apparatus Yamato MP-21 (Yamato Kagaku, Japan) using a capillary; nuclear magnetic resonance spectra ( H-NMR) were determined by JEOL.JNM-FX200 (Nippon Denshi, Japan); molecular weights were determined by JMS-D300 type mass spectrometer (Nippon Denshi, Japan); and infrared absorption spectra (IR) were determined by IRA-1 (Nippon Bunko Kogyo, Japan).
Reference Example 1. l-FN-(Benzvloxvcarbomvl) histidyl 1 -4-phenylpiperazine 7.13 g of N,N'-dibenzyloxycarbonyi histidine, 3.00 g of 4-phenylpiperazine and 16.1 g of N-hydroxybenzotriazole were dissolved in 100 ml of tetrahydrofuran, and to the mixture was added 3.84 g of DCC (dicyclohexylcarbodiimide), and the whole was stirred at a room temperature for three hours. An insoluble matter was filtered off, the filtrate was concentrated under a reduced pressure, and to the concentrate was added 200 ml of ethyl acetate to reform crystals, which were then filtered off. The filtrate was sequentially washed with 20% potassium carbonate aqueous solution and saturated sodium chloride aqueous solution, dried over magnesium sulfate, and concentrated under a reduced pressure.The resulting residue was dissolved in 60 ml of methanol, and after an addition of a 10% ammonium-methanol solution and stirring at a room temperature for 30 minutes, the solution was concentrated under a reduced pressure to obtain a residue, which was then subjected to silica gel chromatography and eluted with chloroform/methanol (50:1 to 10:1) to obtain 6.61 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 2.81 - 3.20 (6H, m), 3.40 - 3.82 (4H, m), 4.95 (lH, m), 5.09 (2H, s), 5.78 (lH, d, J = 8.3 Hz), 6.80 - 6.97 (4H, m), 7.20 - 7.30 (2H, m), 7.34 (5H, s), 7.55 (lH, s).
Reference Example 2. 1-F N- (tert-Butoxycarbonyl histidyl 1 -4-phenylpiperazine 6.61 g of 1-[N-(benzyloxycarbonyl)histidyl]-4 phenylpiperazine was dissolved in 80 ml of methanol, and to the solution was added 4 g of 5% palladium on carbon catalyst with ice cooling, and the mixture was stirred under a hydrogen atmosphere at a room temperature for 20 hours, and filtered to obtain a filtrate, which was then concentrated under a reduced pressure to obtain 4.26 g of a residue.The residue was dissolved in 80 ml of dimethylformamide, and to the solution were sequentially added 6.8 g of tert-butoxycarboxylic acid anhydride and 10 ml of triethylamine, and the mixture was stirred at a room temperature for 90 minutes. 200 ml of ethyl acetate was added to the reaction mixture, which was then washed twice with saturated sodium chloride aqueous solution, dried over magnesium sulfate, and filtered to obtain a filtrate. The filtrate was concentrated under a reduced pressure to obtain a residue, which was then dissolved in 100 ml of methanol, and to the resulting solution was added 20 ml of 10% sodium hydroxide aqueous solution, and the whole was stirred at a room temperature for 30 minutes.The reaction mixture was concentrated under a reduced pressure to one third of the original volume, and after the addition of 150 ml of water, the concentrate was extracted twice with 80 ml each of chloroform, the resulting chloroform phase was dried over magnesium sulfate, filtered, and concentrated under a reduced pressure to obtain a residue. The residue was applied on a silica gel column, and eluted with chloroform/methanol (50:1 to 20:1) to obtain 4.53 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.43 (9H, s), 2.80 - 3.22 (6H, m), 3.40 - 3.83 (4H, m), 4.87 (lH, m), 5.46 (lH, br), 6.86 - 6.93 (4H, m), 7.23 - 7.31 (2H, m), 7.57 (lH, s).
Reference Example 3. 1-F 2- (tert-Butoxycarbonyl- amino)-3-imidazol-4(55-ol-propyll-4-phensl- piperazine A solution of 1.3 g of lithium aluminum hydride in 38 ml of tetrahydrofuran was added to a solution of 4.56 g of aluminum chloride in 38 ml of ethyl ether with ice cooling, and the mixture was stirred for 20 minutes with ice cooling. To the mixture was dropwise added a solution of 4.53 g of 1-[N-(tert-butoxycarbonyl)histidyl]-4-phenylpiperazine in 51 ml of tetrahydrofuran, and stirring for one hour with ice cooling, to the reaction mixture was added 20 ml of 25% potassium carbonate aqueous solution, followed by 100 ml of chloroform to obtain a suspension. The suspension was filtered using silica as a filter aid to obtain a filtrate.After the silica was washed with 208 methanol in chloroform, the combined filtrate was concentrated under a reduced pressure to obtain a residue. The residue was applied to a silica gel column, and eluted with chloroform/methanol (40:1 to 10:1) to obtain 3.1 g of the title compound in a colorless amorphous.
H-NMR (CDCl3 , 6 ppm): 1.44 (9H, s), 2.33 (lH, dd, J = 7.3, 12.2 Hz), 2.47 (1H, dd, J = 7.8, 12.2 Hz), 2.64 (4H, m), 2.93 (2H, m), 3.20 (4H, m), 3.97 (1H, m), 5.10 (lH, br), 6.81 - 6.97 (4H, m), 7.21 - 7.30 (2H, m), 7.58 (1H, s) Example 1. N- Hz1 - 1 - I- (5-Isoauinolinesulfonyl imi- dazol-4{5)-sl-methyll-2-{4-phenolpiPerazinYl) ethyl}-5-isouinolinesulfonamide 3.1 g of the amorphous compound obtained in Reference Example 3 was dissolved in 10 ml of ethyl acetate, and to the solution was added 16 ml of 4N hydrochloric acid in ethyl acetate was added, and the mixture was stirred at a room temperature for 30 hours and evaporated to dryness under a reduced pressure.To the residue were added 70 ml of tetrahydrofuran and 30 ml of chloroform to form a suspension, to which were added 6 g of isoquinolinesulfonic acid chloride and 30 ml of triethylamine, and after stirring at a room temperature for 18 hours, 150 ml of water was added and the whole was extracted twice with 70 ml of chloroform.
The extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to silica gel column, and eluted with chloroform/methanol (80:1 to 60:1) to obtain 1.86 g of the title compound in a colorless amorphous form.
IR (KBr) cm : 1618, 1600, 1490, 1380, 1325, 1210, 1170, 1132, 1073; 1H-NMR (CDCl3 , S ppm): 2.00 - 2.34 (6H, m), 2.59 - 2.81 (6H, m), 3.39 (1H, m), 6.74 - 6.89 (3H, m), 7.04 (1H, d, J = 1.5 Hz), 7.19 - 7.29 (3H, m), 7.69 (1H, t, J = 7.3 Hz), 7.80 (1H, t, J = 7.8 Hz), 7.93 (1H, d, J = 1.5 Hz), 8.21 (1H, d, J = 8.3 Hz), 8.34 (1H, d, J = 8.3 Hz), 8.38 - 8.46 (3H, m), 8.52 (1H, dd, J = 1.0, 7.3 Hz), 8.69 (lH, d, J = 6.3 Hz), 8.77 (1H, d, J = 6.3 Hz), 9.36 (1H, s), 9.39 (lH, s).
Example 2. N-[1-(Imidazol-4(5-yl-methyl)-2-(4- phenylpiperazinyl)ethyl]-5-isoquinoline sulfonamide 250 mg of the amorphous compound obtained in Example 1 was dissolved in a mixture of 1 ml of tetrahydrofuran and 5 ml of methanol, and to the solution 1 ml of 4N sodium hydroxide was added. After stirring at a room temperature for 10 minutes, 20 ml of water was added to the mixture, which was then extracted twice with a mixture of 10 ml of chloroform and 2 ml of isopropanol. The extract was dried over magnesium sulfate, and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column, and eluted with chloroform/methanol (20:1) and chloroform/methanol/triethylamine (20:1:0.2) to obtain 163 mg of the title compound in a colorless amorphous form.
IR (KBr) cm : 1615, 1600, 1490, 1448, 1320, 1225, 1153, 1130; 1H-NMR (CDCl3 , 6 ppm): 2.06 - 2.44 (6H, m), 2.67 - 2.90 (5H, m), 3.02 (1H, dd, J = 5.4, 10.0 Hz), 3.25 (lH, m), 6.74 - 6.90 (4H, m), 7.19 - 7.33 (2H, m), 7.54 (lH, s), 7.74 (1H, t, J = 7.8 Hz), 8.24 (lH, d, J = 7.8 Hz), 8.47 (1H, d, J = 6.4 Hz), 8.52 (1H, dd, J = 1.0, 7.32 Hz), 8.70 (1H, d, J = 5.9 Hz), 9.38 (1H, s).
Example 3. N-Fl- Fl- (5-Isoouinolinesulfonyl imidazol-4 (5 -yl-methyl 1-2- (Dhenvlierazinyl ethyl -N-methyl-5-isoquinoline sulfonamide 1.45 g of the amorphous compound obtained in Example 1 was dissolved in 20 ml of dimethylformamide, and to the solution were sequentially added 120 mg of 60% sodium hydride and 0.2 ml of methyl iodide with ice cooling, and after stirring for 30 minutes with ice cooling, and 30 ml of water was added. After extraction of the reaction mixture with 30 ml of ethyl acetate, the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with chloroform/methanol (80:1) to obtain 616 mg of the title compound in a colorless amorphous form.
IR (KBr) cm : 1618, 1600, 1490, 1380, 1320, 1210, 1170, 1140, 1080; 1H-NMR (CDCl3 , 6 ppm): 2.35 - 2.47 (6H, m), 2.64 (1H, dd, J = 7.8, 14.6 Hz), 2.80 (3H, s), 2.85 - 2.97 (5H, m), 4.36 (lH, m), 6.82 - 6.89 (3H, m), 7.08 (1H, d, J = 1.5 Hz), 7.21 - 7.29 (2H, m), 7.61 (1H, t, J = 7.3 Hz), 7.75 (lH, t, J = 7.8 Hz), 7.87 (1H, d, J = 1.5 Hz), 8.14 (lH, d, J = 7.8 Hz), 8.25 - 8.29 (2H, m), 8.37 - 8.45 (3H, m), 8.64 (1H, d, J = 5.9 Hz), 8.76 (lH, d, J = 6.3 Hz), 9.31 (lH, s), 9.35 (lH, s).
Example 4. N- r 1- ( Imidazol-4(5)-vl-methvl!-2-(4- PhenYlDiPerazinvlBethvll-N-methyl-5-isoauinoline sulfonamide 450 mg of the amorphous compound obtained in Example 3 was dissolved in a mixture of 2 ml of tetrahydrofuran and 10 ml of methanol, and to the solution was added 1 ml of 4 N sodium hydroxide. After stirring at a room temperature for 10 minutes the reaction mixture was worked up according to the same procedure as described in Example 2 to obtain 299 mg of the title compound in a colorless amorphous form.
IR (RBr) cm : 1595, 1490, 1448, 1320, 1225, 1150, 1128i 1H-NMR (CDCl3 , 6 ppm): 2.45 - 2.65 (6H, m), 2.89 (3H, s), 2.90 - 3.08 (6H, m), 4.37 (lH, m), 6.68 (lH, s), 6.82 - 6.90 (3H, m), 7.20 - 7.32 (3H, m), 7.64 (lH, t, J = 7.8 Hz), 8.14 (lH, d, J = 7.8 Hz), 8.31 (lH, d, J = 6.3 Hz), 8.46 (1H, dd, J = 1.0, 7.3 Hz), 8.62 (lH, d, J = 5.9 Hz), 9.29 (lH, s).
Reference Example 4. N- (tert-Butoxycarbonyl -3,4- dibenzyloxvphenylalanine benzyl ester 21.12 g of N-(tert-butoxycarbonyl) DOPA was dissolved in 200 ml of dimethylformamide, and after 50 g of benzyl bromide and 40 g of potassium carbonate were added, the mixture was stirred at a room temperature for 40 hours. After the addition of 400 ml of sodium chloride aqueous solution, the reaction mixture was extracted with 500 ml of ethyl acetate, and the extract was washed twice with saturated sodium chloride aqueous solution, dried over magnesium sulfate, filtered, and concentrated under a reduced pressure. To the resulting residue was added hexane to crystallize the title compound, which was then washed, filtered and dried to obtain 30.0 g of the colorless crystals.
1H-NMR (CDCl3 , 6 ppm): 1.42 (9H, s), 2.99 (2H, d, J = 14.14 Hz), 4.59 (1H, m), 4.98 (1H, brd), 5.05 (2H, s), 5.07 (2H, s), 5.11 (2H, s),6.56 (1H, dd, J = 2.0, 7.8 Hz), 6.71 (1H, d, J = 2.0 Hz), 6.79 (1H, d, J = 7.8 Hz), 7.20 - 7.50.
Reference Example 5. N- (tert-Butoxycarbonyl -3,4- dibenzvloxyphenvlalanine 30.0 g of the crystals obtained in Example 4 was dissolved in 600 ml of methanol, and after the addition of 65 ml of 10% sodium hydroxide, the mixture was stirred at a room temperature for 20 hours, and 1000 ml of water was added. The reaction mixture was adjusted to pH 4 with concentrated hydrochloric acid, and extracted twice with 800 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure to crystallize the title compound, which was then filtered and washed with hexane to obtain 25.2 g of colorless crystals.
H-NMR (CDCl3 , 6 ppm): 1.40 (9H, s), 3.02 (2H, m), 4.49 (lH, brs), 4.88 (lH, brs), 5.11 (4H, s), 6.68 (lH, dd, J = 2.0, 7.8 Hz), 6.76 (1H, d, J = 2.0 Hz), 6.74 (lH, d, J = 7.8 Hz), 7.23 - 7.45 (l0H, m).
Reference Example 6. 1-rN- (tert-Butoxycarbonyl - 3 4-dibenzyloxvphenylalaninvl 1 -4-phenylpiperazine 5.67 g of the crystals obtained in Reference Example 5, 1.9 g of N-phenylpiperazine and 1.53 g of N-hydroxybenzotriazole were dissolved in 80 ml of methylene chloride, and after the addition of 2.4 g of DCC, the mixture was stirred at a room temperature for 18 hours. Resulting insoluble matter was filtered off, and washed with ethyl acetate.
The combined filtrate was concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column, and eluted with hexane/ethyl acetate (2:1) to obtain 6.39 g of the title compound as colorless amorphous.
1H-NMR (CDCl3 , E ppm): 1.44 (9H, s), 2.39 (lH, m), 2.76 - 3.10 (6H, m), 3.30 (1H, m), 3.61 (2H, m), 4.78 (1H, m), 5.03 (2H, s), 5.14 (2H, s), 5.42 (1H, brd, J = 8.3 Hz), 6.69 (1H, dd, J = 2.0, 8.3 Hz), 6.79 - 6.91 (5H, m), 7.20 - 7.48 (12H, m).
Reference Example 7. 1-f2- r N-(tert-Butoxzcar- bonolamino)l-3-(3,4-dibenzvloxophenol) Propol T -4- phenylpiperazine 3.66 g of the colorless amorphous obtained in Reference Example 6 was dissolved in 50 ml of tetrahydrofuran, and the addition of 700 mg of lithium aluminum hydride with ice cooling, the mixture was stirred for 90 minutes with ice cooling, and to the mixture was added water until foaming ended. Then 80 ml of chloroform was added to the reaction mixture to form a suspension, which was then filtered using silica gel as a filter acid to remove insoluble matter. The resulting filtrate was concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column, and eluted with hexane/ethyl acetate (3:1) to obtain 2.67 g of the title compound in colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.43 (9H, s), 2.24 (2H, m), 2.53 (4H, m), 2.79 (2H, m), 3.16 (4H, m), 3.90 (1H, m), 4.58 (1H, brs), 5.13 (2H, s), 5.16 (2H, s), 6.70 (1H, dd, J = 2.0, 8.3 Hz), 6.80 - 6.93 (5H, m), 7.20 7.46 (12H, m).
Reference Example 8. 1-[2-Amino-3-(3,4-diben- zyloxyphenyl] propyl-4-phenylpiperazine 4.35 g of the amorphous compound obtained in Reference Example 7 was dissolved in 20 ml of ethyl acetate, and after the addition of 30 ml of 4 N hydrochloric acid in ethyl acetate, the mixture was stirred at a room temperature for one hour. The reaction mixture was concentrated under a reduced pressure, alkalized with sodium bicarbonate aqueous solution, and extracted twice with 80 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform-methanol (100:1 to 30:1) to obtain 1.64 g of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.27 - 2.68 (8H, m), 3.10 - 3.20 (5H, m), 5.10 (2H, brs), 5.14 (2H, s), 5.17 (2H, s), 6.73 (1H, dd, J = 2.0, 8.3 Hz), 6.81 - 6.94 (5H, m), 7.22 - 7.46 (12H, m).
Example 5. N-;1-f (3 ,4-Dibenzvloxvphenvl)methvl 1-2- (4-phenylpiperazinyl )ethyl-5-isocuinoline sulfon amide 640 mg of the amorphous compound obtained in Reference Example 8 was dissolved in 15 ml of methylene chloride, and to the solution were added 1 ml of triethylamine and 350 mg of 5-isoquinolinesulfonyl chloride with ice cooling, and after stirring for one hour with ice cooling, was added 50 ml of water,. and the mixture was extracted twice with 50 ml of chloroform.
The extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with hexane/ethyl acetate (1:1) to obtain 470 mg of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.08 - 2.24 (6H, m), 2.64 - 2.91 (6H, m), 3.30 (lH, m), 5.08 (2H, s), 5.10 (2H, s), 6.51 (lH, dd, J = 2.0, 8.3 Hz), 6.63 (lH, d, J = 2.0 Hz), 6.71 (lH, d, J = 8.3 Hz), 6.76 - 6.89 (3H, m), 7.21 - 7.43 (12H, m), 7.67 (lH, t, J = 7.8 Hz), 8.18 (lH, d, J = 8.3 Hz), 8.44 (2H, m), 8.67 (1H, d, J = 5.9 Hz), 9.34 (lH, s).
Example 6. N-rl-T3,4-DibenzvloxyPhenvllmethvll-2- (4-phenvliperazinyl ethyl -N-methvl-5-isocruinoline sulfonamide 470 mg of the amorphous compound obtained in Example 5 was dissolved in 8 ml of dimethylformamide, and to the solution were sequentially added 30 mg of 60% sodium hydride and 0.1 ml of methyl iodide with ice cooling, and after stirring for two hours with ice cooling was added saturated sodium chloride, and the mixture was extracted with 50 ml of ethyl acetate. The extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with hexane/ethyl acetate (1:1) to obtain 413 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 2.34 (1H, dd, J = 6.35, 13.2 Hz), 2.42 - 2.60 (5H, m), 2.65 (1H, dd, J = 7.3, 14.2 Hz), 2.81 (1H, dd, J = 6.4, 14.2 Hz), 2.86 (3H, s), 2.99 (4H, m), 4.22 (1H, m), 5.03 (2H, s), 5.10 (2K, s), 6.54 (1H, dd, J = 2.0, 8.3 Hz), 6.61 (1H, d, J = 2.0 Hz), 6.63 (1H, d, J = 8.3 Hz), 6.82 - 6.90 (3H, m), 7.19 - 7.53 (13H, m), 8.05 (lH, d, J = 8.3 Hz), 8.24 (1H, dd, J = 1.0, 7.3 Hz), 8.30 (1H, d, J = 5.9 Hz), 8.60 (1H, d, J = 5.9 Hz), 9.24 (1H, d, J = 1.0 Hz).
Example 7. N-{l-r(3 4-DihydroxvPhensl)methyll-2- (4-phenylpiperazinyl)ethyl}-N-methyl-isquinoline sulfonamide 310 mg of the amorphous compound obtained in Example 6 was dissolved in 2 ml of 1,2-ethanedithiol, and to the solution were added 1 ml of boron trifluoride/ethyl ether, and after stirring at a room temperature for 18 hours, was added saturated sodium bicarbonate aqueous solution, and the reaction mixture was extracted twice with a mixture of chloroform and methanol (10:1). The extract was dried over magnesium sulfate, and concentrated under a reduced pressure to obtain a residue, which was applied to a silica gel column and eluted with chloroform/methanol (80:1 to 20:1) to obtain 148 mg of the title compound in a colorless amorphous form.
IR (KBr) cm 1 1600, 1495, 1448, 1328, 1230, 1155, 1130; H-NMR (CDCl3 , 6 ppm): 2.41 (1H, dd, J = 10.25, 14.65 Hz), 2.50 - 2.98 (7H, m), 3.01 (3H, s), 3.17 (4H, m), 4.06 (1H, m), 6.12 (1H, dd, J = 20, 8.3 Hz), 6.20 (1H, d, J = 8.3 Hz), 6.28 (1H, d, J = 2.0 Hz), 6.82 6.95 (3H, m), 7.26 (2H, m), 7.62 (1H, t, J = 7.8 Hz), 8.09 (1H, d, J = 6.8 Hz), 8.13 (1H, d, J = 9.3 Hz), 8.30 (1H, d, J = 6.8 Hz), 8.41 (1H, d, J = 4.9 Hz), 9.25 (1H, s).
Example 8. N-(1-r (3 ,4-Dihydroxvphenyl)methyll-2- (4-phenylpiperazinvl ! ethyli-5-isoauinoline sulfonamide The amorphous compound obtained in Example 5 was treated according to the procedure as described in Example 7 to obtain the title compound in colorless amorphous form.
IR (RBr) cm 1 1610, 1600, 1490, 1445, 1320, 1220, 1150, 1128; 1H-NMR (CDC13 , 6 ppm): 2.30 - 2.60 (6H, m), 2.74 - 3.02 (6H, m), 3.36 (1H, m), 6.15 (1H, d, J = 8.3 Hz), 6.33 (1H, d, J = 8.3 Hz), 6.36 (1H, s), 6.76 - 6.90 (3H, m), 7.19 - 7.29 (2H, m), 7.65 (1H, t, J = 7.8 Hz), 8.16 (1H, d, J = 8.3 Hz), 8.33 (lH, d, J = 6.5 Hz), 8.39 (lH, d, J = 7.3 Hz), 8.51 (1H, d, J = 5.5 Hz), 9.28 (1H, s).
Reference Example 9. 6 7-Dibenzoloxw-3-f(4-Phenol- piperazinylmethyl)-1,2,3,4-tetrahydroisoaquinoline 1.00 g of the amorphous compound obtained in Reference Example 8 was dissolved in 2 ml of tetrahydrofuran, and to the solution was added 0.25 ml of 37% formalin. After stirring at a room temperature for 30 minutes, 600 mg of 12 N hydrochloric acid was added to the mixture, which was then stirred at a room temperature for two hours. After the addition of saturated sodium bicarbonate aqueous solution, the reaction mixture was extracted twice with 20 ml of chloroform.
The extract was dried over magnesium sulfate, and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 585 mg of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.28 - 2.38 (8H, m), 3.02 (1H, m), 3.21 (4H, m), 3.95 (2H, s), 5.11 (4H, s), 6.63 (1H, s), 6.68 (1H, s), 6.80 - 6.95 (3H, m), 7.20 - 7.46 (12H, m).
Example 9. 6 7-Dibenzvloxv-2-(5-isoquinolinesul- fonyl)-3-r (4-phenvlpiperazinvl)methyll-1,2,3,4- tetrahodroisoquinoline 580 mg of the amorphous compound obtained in Reference Example 9 was dissolved in 10 ml of methylene chloride, and to the solution were added 1 ml of triethylamine and 400 mg of 5-isoquinoline sulfonyl chloride.HCl with ice cooling. The mixture was stirred at a room temperature for two hours, and after the addition of 20 ml of water, extracted twice with 10 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with hexane/ethyl acetate (1:1) to obtain 610 mg of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.31 (1H, dd, J = 7.8, 11.6 Hz), 2.43 (1H, dd, J = 6.8, 11.6 Hz), 2.53 (4H, m), 2.70 (IR, dd, J = 2.0, 16.2 Hz), 2.87 (1H, dd, J = 4.2, 16.2 Hz), 3.05 (4H, m), 4.26 (1H, d, J = 15.6 Hz), 4.48 (1H, d, J = 15.6 Hz), 4.49 (1H, m), 5.06 (2H, s), 5.07 (2H, s), 6.56 (1H, s), 6.60 (1H, s), 6.80 - 6.90 (3H, m), 7.20 - 7.95 (12H, m), 7.64 (1H, t, J = 7.8 Hz), 8.15 (lH, d, J = 7.81 Hz), 8.37 (1H, d, J = 5.9 Hz), 8.48 (1H, dd, J = 1.0, 7.3 Hz), 8.64 (1H, d, J = 6.4 Hz), 9.30 (lH, d, J = 1.0 HZ?- Example 10. 6 7-Dihydroxo-2-(5-isoquinoline- sulfonyl)-3-F (4-phenvlpiperazinvlznethvl1-1,2,3,4- tetrahvdroisosuinoline To 314 mg of the amorphous compound obtained in Example 9, were added 2 ml of 1,2-ethanedithiol and 1 ml of boron trifluoride/ethyl ether, and the mixture was stirred at a room temperature for 18 hours. After the addition of saturated sodium bicarbonate aqueous solution, the reaction mixture was extracted twice with a mixture of chloroform and methanol (1:1), and the extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with chloroform/methanol (50:1 to 20:1) to obtain 213 mg of the title compound in a colorless amorphous form.
IR (KBr) cm : 1610, 1600, 1490, 1445, 1320, 1225, 1150, 1130; 1H-NMR (CDC13 , 6 ppm): 2.35 - 2.80 (8H, m), 3.11 (4H, m), 4.24 (lH, d, J = 16.1 Hz), 4.40 (lH, d, J = 16.1 Hz), 4.55 (lH, m), 6.45 (2H, s), 6.80 - 6.9.0 (3K, m), 7.20 - 7.28 (2H, m), 7.67 (lH, t, J = 7.8 Hz), 8.14 (lH, d, J = 8.5 Hz), 8.40 (lH, d, J = 6.3 Hz), 8.50 (1H, dd, J = 1.0, 7.3 Hz), 8.59 (lH, d, J = 6.4 Hz), 9.25 (1H, d, J = 1.0 Hz).
Reference Example 10. 1-[N-(tert-Butoxycarbonyl)- 1-FN-(tert-Butoxycarbonyl )- p-nitrophenvlalanvl I -4-phenylpiperazine 7.03 g of p-nitrophenylalanine was suspended in 70 ml of 1,4-dioxane, and to the suspension were added 28 ml of 10% sodium hydroxide aqueous solution and 7.5 g of di-tert-butyl-dicarbonate, and the mixture was stirred at a room temperature for 30 minutes. 200 ml of water and 7 ml of 12 N hydrochloric acid were added to the reaction mixture, which was then extracted with 150 ml of ethyl acetate, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate, and concentrated under a reduced pressure. The resulting residue was dissolved in 150 ml of tetrahydrofuran, and to the solution were added 6.0 g of N-phenylpiperazine and 5.5 g of N-hydroxybenzotriazole, and further added 7.6 g of DCC.After stirring at a room temperature for three hours, the reaction mixture was filtered to remove insoluble matter and the filtrate was concentrated under a reduced pressure, and the resulting residue was dissolved in 200 ml of ethyl acetate. The solution was sequentially washed with 10% potassium carbonate aqueous solution and saturated sodium chloride aqueous solution, dried over magnesium sulfate, and concentrated under a reduced pressure to obtain a residue, which was then applied to a silicon gel column and eluted with hexane/ethyl acetate (2:1) to obtain 11.1 g of the title compound as pale yellow crystals.
1H-NMR (CDCl3 , 6 ppm): 1.40 (9H, s), 2.83 - 3.20 (6H, m), 3.37 (lH, m), 3.57 - 3.70 (3H, m), 3.84 (1H, m), 4.92 (1H, m), 5.40 (1H, d, J = 8.3 Hz), 6.85 - 6.95 (3H, m), 7.24 - 7.32 (2H, m), 7.38 (2H, d, J = 8.8 Hz), 8.16 (2H, d, J = 8.8 Hz).
Example 11. 1- r N-(5-Isoquinolinesulfonyl)-D- nitrophenvlalanyl 1-4 -phenylpiperazine 11.0 g of the crystals obtained in Reference Example 10 was dissolved in 100 ml of ethyl acetate, and after the addition of 100 ml of 4 N hydrochloric acid in ethyl acetate, the reaction mixture was stirred at a room temperature for one hour and concentrated to dryness under a reduced pressure. To the residue was added 200 ml of saturated sodium bicarbonate, and the mixture was extracted twice with 100 ml of chloroform.
The extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with chloroform/methanol (80:1 to 10:1) to obtain free amine. The free amine was dissolved in 100 ml of methylene chloride, and to the solution were sequentially added 8.5 g of 5-isoquinoline sulfonyl chloride.HCl and 20 ml of triethylamine. The reaction mixture was stirred at a room temperature for 18 hours, and after the addition of water, extracted twice with 100 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1) to obtain 9.66 g of the title compound as colorless crystals.
Melting point: 184 - 1880C (decomposed); IR (KBr) cm : 1660, 1600, 1520, 1420, 1345, 1325, 1230, 1150, 1135; 1H-NMR (CDC13 , 6 ppm): 2.72 - 3.06 (6H, m), 3.20 - 3.61 (4H, m), 4.46 (lH, m), 6.06 (1H, br), 6.83 (2H, d, J = 7.8 Hz), 6.94 (1H, t, J = 7.3 Hz), 7.10 (2H, t, J = 8.8 Hz), 7.29 (2H, m), 7.56 (1H, t, J = 7.8 Hz), 7.82 (2H, d, J = 8.8 Hz), 8.09 (1H, d, J = 7.8 Hz), 8.22 8.29 (2H, m), 8.71 (lH, d, J = 6.3 Hz), 9.26 (1H, s).
Example 12. 1- r N-(5-Isoauinolinesulfonvl)-N- methvl-p-nitrophenylalanvl 1 -4-phenylpiperazine 5.87 g of the crystals obtained in Example 11 was dissolved in 60 ml of dimethylformamide, and to the solution were sequentially added 500 mg of 60% sodium hydride and 1.5 ml of methyl iodide with ice cooling.
After stirring for two hours with ice cooling, water was added to the reaction mixture, which was then extracted with 150 ml of ethyl acetate. The extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 5.93 g of the title compound in a yellow amorphous form.
-l IR (KBr) cm : 1640, 1600, 1535, 1445, 1340, 1225, 1150, 1125; 1H-NMR (CDC13 , 6 ppm): 2.60 (1H, dd, J = 4.9, 12.7 Hz), 2.83 (lH, m), 2.92 - 3.06 (3H, m), 3.06 (3H, s), 3.40 (lH, dd, J = 7.8, 13.2 Hz), 3.46 - 3.63 (2H, m), 3.70 - 3.88 (2H, m), 5.23 (lH, dd, J = 4.9, 9.8 Hz), 6.82 (2H, d, J = 7.8 Hz), 6.91 (1K, t, J = 7.3 Hz), 7.22 - 7.30 (4H, m), 7.73 (1H, t, J = 7.8 Hz), 8.07 (2H, d, J = 8.8 Hz), 8.25 (1H, d, J = 8.3 Hz), 8.36 (1H, dd, J = 1.0, 7.3 Hz), 8.48 (1H, d, J = 6.4 Hz), 8.71 (1H, d, J = 6.4 Hz), 9.37 (1H, d, J = 1.0 Hz).
Example 13. 1- r p-Amino-N-(5-isoquinolinesulfonsl)- N-methylalanyl 1-4 -phenvipiperazine 6.08 g of the amorphous compound obtained in Example 12 was dissolved in 70 ml of methanol, and to the solution were added 5 ml of 12 N hydrochloric acid and 30 ml of water, and then 5 g of 5% palladium on carbon. The mixture was stirred at a room temperature under a hydrogen atmosphere for 30 minutes, and filtered to remove insoluble matter, and the filtrate was concentrated under a reduced pressure, and to the residue was added 150 ml of saturated sodium bicarbonate aqueous solution, and the mixture was extracted twice with 200 ml of chloroform.The extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with chloroform/methanol (50:1) to obtain 3.32 g of the title compound in a yellow amorphous form.
IR (KBr) cm : 1635, 1600, 1495, 1445, 1325, 1220, 1150, 1125; 1H-NMR (CDC13 , E ppm): 2.47 - 2.56 (2H, m), 2.87 - 3.22 (4H, m), 3.14 (3H, s), 3.33 - 3.75 (4H, m), 5.14 (1H, dd, J = 4.9, 9.8 Hz), 6.50 (2H, d, J = 8.3 Hz), 6.80 - 6.92 (5H, m), 7.22 - 7.34 (2H, m), 7.69 (1H, t, J = 7.8 Hz), 8.20 (1H, d, J = 8.3 Hz), 8.36 (1H, dd, J = 1.5, 7.3 Hz), 8.40 (1H, d, J = 5.9 Hz), 8.68 (1H, d, J = 6.4 Hz), 9.34 (lH, s).
Example 14.
3.75 g of the crystal prepared in Example 11 was treated according to the procedure as described in Example 13 to obtain 2.17 g of l-[p-amino-N (5-isoquinolinesulfonyl ) phenylalanyl) -4-phenylpiperazine as yellow crystals.
IR (KBr) cm 1 1635, 1600, 1495, 1440, 1320, 1225, 1155, 1135; 1H-NMR (CDC13 , 6 ppm): 2.41 (1H, m), 2.59 - 3.07 (6H, m), 3.17 (1H, m), 3.33 (1H, m), 3.51 (1H, m), 4.35 (1H, m), 5.95 (1H, d, J = 9.3 Hz), 6.38 (2H, d, J = 8.3 Hz), 6.75 (2H, d, J = 8.3 Hz), 6.78 (2H, d, J = 7.8 Hz), 6.91 (1H, t, J = 7.3 Hz), 7.22 - 7.30 (2H, m), 7.60 (1H, t, J = 8.3 Hz), 8.12 (1H, d, J = 8.3 Hz), 8.30 - 8.35 (2H, m), 8.71 (1H, d, J = 5.9 Hz), 9.30 (1H, s).
Example 15. 1-FN- (5-Isouinolinesulfonyl )-p- (p- toluenesulfohylamino ! phenylalanyl -4-phenyl piperazine 200 mg of the crystals obtained in Example 14 was dissolved in 5 ml of pyridine, and to the solution was added 90 mg of p-toluenesulfonylchloride with ice cooling, and the mixture was stirred for one hour with ice cooling and poured to 30 ml of saturated sodium bicarbonate aqueous solution. The mixture was extracted twice with 15 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with chloroform/methanol (80:1 to 50:1) to obtain 128 mg of the title compound.
IR (KBr) cm : 1635, 1600, 1335, 1225, 1155, 1090; 1H-NMR (CDC13 , 6 ppm): 2.30 (3H, s), 2.65 - 2.80 (4H, m), 2.83 - 3.04 (2H, m), 3.17 - 3.50 (4H, m), 4.33 (1H, m), 6.14 (1H, d, J = 9.3 Hz), 6.70 - 6.83 (6H, m), 6.93 (1H, t, J = 7.3 Hz), 6.77 (1H, s), 7.17 (2H, d, J = 8.3 Hz), 7.26 - 7.36 (2H, m), 7.59 (1H, t, J = 7.3 Hz), 7.59 (2H, d, J = 8.3 Hz), 8.13 (lH, d, J = 7.8 Hz), 8.30 (2H, m), 8.64 (1H, br), 9.31 (lH, br).
Example 16.
The same procedure as described in Example 15 was repeated, except that 200 mg of 5-isoquinolinesulfonyl chloride.RCl and 300 mg of the crystals obtained in Example 14 were used and elution was carried out with chloroform/methanol (40:1 to 20:1), to obtain 372 mg of 1- [N- (5-isoquinolinesulfonyl ) -p- (5-isoquinolinesulfonyl- amino )phenylalanyl) -4-phenylpiperazine.
IR (KBr) cm 1 1630, 1600, 1340, 1225, 1155, 1135; 1H-NMR (CDC13 , 6 ppm): 2.35 - 3.07 (9H, m), 3.30 (lH, m), 4.26 (lH, m), 6.67 - 6.84 (6H, m), 6.89 - 6.96 (2H, m), 7.23 (2H, t, J = 8.8 Hz), 7.52 (lH, t, J = 7.8 Hz), 7.54 (lH, t, J = 7.8 Hz), 7.98 (1H, d, J = 7.8 Hz), 8.07 (lH, d, J = 8.3 Hz), 8.25 (lH, d, J = 6.8 Hz), 8.31 - 8.36 (2H, m), 8.54 (1H, d, J = 6.3 Hz), 8.65 (2H, d, J = 6.4 Hz), 9.17 (1H, s), 9.26 (1H, s), 10.06 (1H, s).
Example 17.
The same procedure as described in Example 15 was repeated, except that 200 mg of 1-naphtharenesulfonyl chloride and 360 mg of the crystals obtained in Example 14 were used as starting materials and elution was carried out using chloroform/methanol (80:1 to 50:1), to obtain 385 mg of 1-EN-(5-isoquinolinesulfonyl)-p-(1- naphtharenesulfonylamino)phenylalanyl]-4-phenyl- piperazine.
1H-NMR (CDC13 , 6 ppm): 2.40 - 2.74 (6H, m), 2.80 - 3.04 (3K, m) 3.33 (lH, m), 4.24 (1H, m), 6.68 - 6.82 (6H, m), 6.92 (1H, t, J = 7.3 Hz), 7.12 (lH, d, J = 9.3 Hz), 7.26 - 7.57 (SH, m), 7.65 (1H, m), 7.78 (1H, d, J = 8.3 Hz), 7.88 (lH, d, J = 7.8 Hz), 7.99 (1H, d, J = 8.3 Hz), 8.16 (lH, dd, J = 1.0, 7.3 Hz), 8.21 (1H, dd, J = 1.0, 7.3 Hz), 8.36 (1H, d, J = 5.9 Hz), 8.65 (lH, d, J = 6.4 Hz), 8.77 (1H, d, J = 8.8 Hz), 9.22 (1H, s), 9.88 (1H, s).
Example 18.
The same procedure as described in Example 15 was repeated, except that 0.07 ml of methanesulfonyl chloride and 360 mg of the crystals obtained in Example 14 were used as starting materials and elution was carried out using chloroform/methanol (50:1 to 30:1), to obtain 356 mg of 1-[N-(5-isoquinolinesulfonyl)-p-(methane- sulfonylamino ) phenylalanyl ) -4-phenylpiperazine.
IR (KBr) cm 1 1635, 1600, 1330, 1225, 1150; H-NMR (CDC13 , 6 ppm): 2.38 (1H, m), 2.72 (3H, s), 2.70 - 2.90 (6H, m), 3.04 - 3.21 (3H, m), 3.42 (1H, m), 4.39 (1H, m), 6.78 (2H, d, J = 7.8 Hz), 6.88 (lH, t, J = 7.3 Hz), 6.92 (2H, d, J = 8.3 Hz), 7.00 (2H, d, J = 8.3 Hz), 7.20 - 7.30 (3H, m), 7.62 (1H, t, J = 7.8 Hz), 8.16 (1K, d, J = 8.3 Hz), 8.32 (1H, dd, J = 1.0, 7.3 Hz), 8.42 (1K, d, J = 5.9 Hz), 8.69 (lH, d, J = 6.4 Hz), 9.15 (1H, s), 9.31 (lH, s).
Example 19. l-rN-(5-Isoquinolinesulfonvl)-p- methanesulfonvlamino-N-methylphenvlalanvl 1-4- phenylpiperazine 700 mg of the amorphous compound obtained in Example 13 was dissolved in 7 ml of pyridine, and to the solution was added 0.13 ml of methanesulfonyl chloride with ice cooling, and the mixture was stirred for one hour with ice cooling and poured to 50 ml of saturated sodium bicarbonate aqueous solution. The mixture was extracted twice with 30 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1) to obtain 790 mg of the title compound.
IR (KBr) cm 1 1635, 1595, 1325, 1220, 1145; H-NMR (CDC13 , 6 ppm): 2.48 - 2.59 (2H, m), 2.83 (3H, s), 2.85 - 3.10 (3H, m), 3.12 (3H, s), 3.22 (lH, dd, J = 9.8, 13.2 Hz), 3.44 - 3.80 (4H, m), 5.16 (1H, dd, J = 5.4, 9.8 Hz), 6.80 - 6.93 (4H, m), 7.04 (4H, s), 7.26 (2H, t, J = 8.3 Hz), 7.72 (1H, t, J = 7.8 Hz), 8.23 (1H, d, J = 8.3 Hz), 8.35 (lH, dd, J = 1.0, 7.3 Hz) 8.42 (ire, d, J - 5.9 Hz), 8.68 (lH, d, J = 5.9 Hz), 9.36 (lH, s).
Example 20.
The same procedure as described in Example 19 was repeated, except that 360 mg of l-naphtharenesulfonyl chloride and 700 mg of the amorphous compound obtained in Example 13 were used as starting materials, and elution was carried out using chloroform/methanol (100:1) to obtain 770 mg of 1-[N-(5-isoquinolinesulfonyl)-N-methyl-p-(1-naphtharenesulfonylamino)phenylalanyl]-4-phenylpiperazine.
IR (KBr) cm : 1635, 1595, 1440, 1330, 1220, 1150, 1120; 1H-NMR (CDC13 , 6 ppm): 2.42 (lH, dd, J = 4.9, 12.7 Hz), 2.63 (1H, m), 2.85 - 3.17 (4H, m), 3.07 (3H, s), 3.32 - 3.73 (4H, m), 5.04 (1H, dd, J = 5.4, 10.3 Hz), 6.73 - 6.96 (7H, m), 7.05 (1H, br), 7.30 7.41 (3H, m) 7.54 - 7.70 (3H, m), 7.88 (lH, d, J = 7.8 Hz), 7.95 (1H, d, J = 8.3 Hz), 8.12 (lH, dd, J = 1.0, 7.3 Hz), 8.16 (1H, d, J = 8.3 Hz), 8.27 (1H, dd, J = 1.5, 7.3 Hz), 8.37 (lH, d, J = 6.3 Hz), 8.62 - 8.68 (2H, m), 9.32 (lH, s).
Example 21.
The same procedure as described in Example 19 was repeated except that 320 mg of 5-isoquinolinesulfonyl chloride.HCL as a sulfonating agent, 500 mg of the amorphous compound obtained in Example 13, 5 ml of pyridine and chloroform/methanol (80:1 to 50:1) as an eluent were used, to obtain 498 mg of 1-[N-(5-iso- quinolinesulfonyl)-p-( 5 -isoquinolinesulfonylamino)-N- methylphenylalanyl]-4-phenylpiperazine.
IR (KBr) cm 1 1640, 1595, 1330, 1225, 1155, 1135 1H-NMR (CDC13 , s ppm): 2.44 (1H, dd, J = 4.9, 13.2 Hz), 2.61 (1H, m), 2.85 - 3.26 (5H, m), 3.05 (3H, s), 3.40 - 3.70 (3H, m), 5.06 (1H, dd, J = 4.9, 9.8 Hz), 6.77 (2H, d, J = 8.8 Hz), 6.79 - 6.97 (5K, m), 7.31 (2H, t, J = 7.3 Hz), 7.53 (lH, t, J = 8.3 Hz), 7.63 (1H, t, J = 8.8 Hz), 8 08 (1H, d, J = 8.3 Hz), 8.20 (1H, d, J = 8.3 Hz), 8.27 - 8.32 (2H, m), 8.37 (2H, d, J = 6.4 Hz), 8.64 (1H, d, J = 6.4 Hz), 8.67 (lH, d, J = 6.4 Hz), 9.29 (1H, s), 9.34 (lH, s) Example 22.
The same procedure as described in Example 19 was repeated except that 300 mg of p-toluenesulfonyl chloride as a sulfonating agent, 700 mg of the amorphous compound obtained in Example 13, 10 ml of pyridine as an eluate and chloroform/methanol (100:1) were used, to obtain 812 mg of 1-[N-(5-isoquinolinesulfonyl)-N-methylp-(p-toluenesulfonylamino)phenylalanyl]-4-phenyl- piperazine.
IR (KBr) cm : 1635, 1595, 1440, 1325, 1220, 1150; 1H-NMR (CDC13 , s ppm): 2.32 (3H, s), 2.50 (1H, dd, J = 4.9, 12.7 Hz), 2.68 (1H, m), 2.90 - 3.03 (4H, m), 3.10 (3H, s), 3.29 (1H, m), 3.42 - 3.73 (3H, m), 5.12 (1H, dd, J = 5.4, 9.8 Hz), 6.79 - 6.97 (7H, m), 7.17 (2H, d, J = 8.3 Hz), 7.28 (2H, t, J = 7.3 Hz), 7.61 (2H, d, J = 8.3 Hz), 7.69 (lH, t, J = 7.8 Hz), 8.21 (1H, d, J = 8.3 Hz), 8.31 (1H, dd, J r 1.5, 7.3 Hz), .8.40 (1H, d, J = 5.9 Hz), 8.65 (1H, d, J = 6.4 Hz), 9.35 (1H, s).
Example 23. 1-{N-(5-Isoquinolinesulfonyl)-p- [N'- (5-isoquinolinesulfonol)-N r -methvlaminol-N-methyl- phenylalanyl -4 -phenylpiperazine 306 mg of the product in Example 21 was dissolved in 5 ml of dimethylformamide, and to the solution were added 25 mg of 60% sodium hydride and 0.1 ml of hydrogen iodide with ice cooling, and the mixture was stirred for one hour with ice cooling. After the addition of 30 ml of saturated sodium chloride, the mixture was extracted with 30 ml of ethyl acetate, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (80:1) to obtain 266 mg of the title compound.
IR (KBr) cm 1 1640, 1600, 1445, 1340, 1225, 1150, 1130; 1H-NMR (CDC13 , 6 ppm): 2.41 - 2.61 (2H, m), 2.83 - 3.09 (3H, m), 3.07 (6H, s), 3.27 (1H, dd, J = 10.7, 13.2 Hz), 3.43 (1H, m), 3.56 - 3.71 (3H, m) 5.18 (1H, dd, J = 4.4, 10.7 Hz), 6.80 - 6.91 (3H, m), 6.94 (2H, d, J = 8.8 Hz), 7.00 (2H, d, J = 8.8 Hz), 7.21 - 7.30 (2H, m), 7.60 (1H, t, J = 7.8 Hz), 7.73 (1H, t, J = 7.8 Hz), 7.98 (1H, d, J = 5.9 Hz), 8.14 - 8.23 (3H, m), 8.36 (1H, d, J = 8.4 Hz), 8.40 (1H, d, J = 5.9 Hz), 8.46 (1H, d, J = 6.4 Hz), 8.69 (1H, d, J = 6.4 Hz), 9.29 (1H, s), 9.37 (1H, s).
Example 24.
The same procedure as described in Example 23 was repeated except that 594 mg of the product of Example 19 was dissolved in 6 ml of dimethylformamide and to the solution were added 60 mg of 60% sodium hydride and 0.1 ml of methyl iodide, to obtain 450 mg of 1-[N-(5 isoquinolinesulfonyl ) -p- (N' -methanesulfonyl-N'-methyl- amino) N-methylphenylalanyl]-4-phenylpiperazine.
IR (XBr) cm : 1635, 1595, 1445, 1335, 1225, 1150, 1140; 1H-NMR (CDC13, 6 ppm): 2.36 (1H, m), 2.50 (lH, dd, J = 3.9, 12.2 Hz), 2.64 (3H, s), 2.81 (lH, m), 2.96 3.16 (2H, m), 3.11 (3H, s), 3.16 (3H, s), 3.31 (lH, dd, J = 10.7, 12.7 Hz), 3.37 - 3.62 (3H, m), 3.78 (lH, m), 5.20 (1H, dd, J = 4.4, 10.7 Hz), 6.80 (2H, d, J = 7.8 Hz), 6.88 (lH, t, J = 7.3 Hz), 7.12 (2H, d, J = 8.8 Hz), 7.21 - 7.31 (4H, m), 7.74 (lH, t, J = 7.8 Hz), 8.24 (1H, d, J = 7.8 Hz), 8.38 (1H, dd, J = 1.0, 7.3 Hz), 8.47 (1H, d, J = 6.4 Hz), 8.71 (1H, d, J = 6.4 Hz), 9.37 (1H, s).
Example 25.
The same procedure as described in Example 23 was repeated, except that 587 mg of the product of Example 20 was dissolved in 6 ml of dimethylformamide and to the solution were added 50 mg of 60% sodium hydride and 0.1 ml of methyl iodide, and elution was carried out using chloroform/methanol (100:1), to obtain 490 mg of 1-{N-(5-isoquinolinesulfonyl)-N-methyl-p-[N'-methyl-N' (l-naphtharenesulfonyl)amino]phenylalanyl}-4-phenylpiperazine.
IR (KBr) cm 1: 1640, 1600, 1440, 1330, 1220, 1150, 1125; 1H-NMR (CDC13 , 6 ppm): 2.47 (lH, dd, J = 4.4, 12.7 Hz), 2.53 (lH, m), 2.80 - 3.07 (3H, m), 3.07 (3H, s), 3.08 (3H, s), 3.27 (lH, dd, J = 10.3, 12.7 Hz), 3.38 (1H, m), 3.51 - 3.65 (3H, m), 5.17 (lH, dd, J = 4.4, 10.3 Hz), 6.81 (2H, d, J = 8.8 Hz), 6.88 (lH, t, J = 7.3 Hz), 6.98 (4H, 5), 7.24 (2H, dd, J = 7.3, 8.8Hz), 7.38 - 7.57 (3H, m), 7.72 (1H, d, J = 7.3 Hz), 7.88 (1H, d, J = 7.8 Hz), 8.01 (1H, d, J = 8.3 Hz), 8.04 (1H, d, J = 7.3 Hz), 8.23 (1H, d, J = 8.3 Hz), 8.32 - 8.37 (2H, m), 8.46 (1H, d, J = 5.9 Hz), 8.68 (lH, d, J = 6.3 Hz), 9.36 (1H, s).
Example 26.
The same procedure as described in Example 23 was repeated, except that 650 mg of the product of Example 22 was dissolved in 10 ml of dimethylformamide, and to the solution were added 60 mg of 60% sodium hydride and 0.1 ml of methyl iodide, and elution was carried out using chloroform/methanol (100:1y, to obtain 603 mg of l-{N-(5-isoquinolinesulfonyl)-N-methyl-p-[N'-methyl N' -(p-toluenesulfonyl)amino)phenylalanyl}-4- phenylpiperazine.
IR (KBr) cm 1: 1640, 1600, 1440, 1335, 1220, 1145; 1H-NMR (CDC13 , s ppm): 2.37 (3H, s), 2.52 (1H, dd, J = 4.9, 12.7 Hz), 2.55 (1H, m), 2.80 - 3.10 (3H, m), 2.99 (3H, s), 3.11 (3H, s), 3.28 (lH, dd, J = 10.3, 12.7 Hz), 3.40 (lH, m), 3.50 - 3.68 (3H, m), 5.20 (1H, dd, J = 4.9, 10.3 Hz), 6.81 (2H, d, J = 8.3 Hz), 6.88 (1H, t, J = 7.3 Hz), 6.98 (2H, d, J = 8.8 Hz) 7.05 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 8.3 Hz), 7.24 (2H, dd, J = 7.3, 8.3 Hz), 7.37 (2H, d, J = 8.3 Hz), 7.73 (lH, t, J = 7.8 Hz), 8.23 (1H, d, J = 8.3 Hz), 8.36 (1H, dd, J = 1.0, 7.8 Hz), 8.46 (1H, d, J = 6.4 Hz), 8.70 (1H, d, J = 6.4 Hz) 9.36 (lH, s).
Reference Example 11. l-(N-Benzyloxycarbonyl tyrosyl ! -4- ( tert-butoxvcarbonvl piperazine 21.31 g of N-benzyloxycarbonyltyrosine and 11.79 of N- (tert-butoxycarbonyl )piperazine were dissolved in a mixed solvent of 200 ml of methylene chloride and 100 ml of ethyl acetate, and to the solution was added 14 g of DCC.After stirring at a room temperature for 40 hours, precipitated insoluble matter was filtered off, and the filtrate was concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (1:1) to obtain 23.9 g of the title compound in a colorless amorphous form 1H-NMR (CDC13 , 6 ppm): 1.45 (9H, s), 2.80 - 3.02 (4H, m), 3.14 - 3.39 (4H, m), 3.49 (2H, m), 4.83 (1H, m), 5.08 (lH, d, J = 12 Hz), 5.10 (lH, d, J = 12. Hz), 5.69 (1H, d, J = 8.8 Hz), 6.17 (lH, br), 6.72 (2H, d, J = 8.8 Hz), 7.01 (2H, d, J = 8.3 Hz), 7;34 (5K, s).
Example 27. 1- r N ItrN,O-bis(5-isoquinolinesulfonyl) tyrosyl]-4-(tert-butoxycarbonyl)piperazine 1.00 g of the amorphous compound obtained in Reference Example 11 was dissolved in 20 ml of methanol, to the solution was added 500 mg of 5% palladium on carbon, and the mixture was stirred under a hydrogen atmosphere at a room temperature for 5 hours. After removing insoluble matter by filtration, the filtrate was concentrated under a reduced pressure. To the resulting residue were added sequentially 30 ml of tetrahydrofuran, 630 mg of 5-isoquinolinesulfonyl chloride.HCl and 1.4 ml of triethylamine, and the mixture was stirred at a room temperature for 50 hours, and after the addition of 100 ml of water, extracted twice with 50 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure.The resulting residue was then applied to a silica gel column and eluted with chloroform/methanol (50:1 to 25:1) to obtain 1.38 g of the title compound in a yellow amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.45 (9H, s), 2.53 - 3.18 (lOH, m), 4.29 (1H, m), 6.05 (1H, d, J = 9.3 Hz), 6.61 (2H, d, J = 8.8 Hz), 6.85 (2H, d, J = 8.8 Hz), 7.62 (1H, t, J = 7.8 Hz), 7.66 (lH, t, J = 7.8 Hz), 8.19 (2H, d, J = 7.8 Hz), 8.26 - 8.31 (3H, m), 8.52 (1H, d, J = 5.9 Hz), 8.69 (1H, d, J = 5.9 Hz), 8.84 (1H, d, J = 6.4 Hz), 9.33 (1H, s), 9.43 (1H, s) Example 28. 1-F N, 0-bis (5-isoauinolinesulfonvl) tyrosyl 1 piperazine 366 mg of the amorphous compound prepared in Example 27 was dissolved in 3 ml of chloroform, and to the solution was added 5 ml of 3N hydrochloric acid/ ethyl acetate. After stirring at a room temperature for one hour, the mixture was concentrated under a reduced pressure, and to resulting residue was added 50 .ml of saturated sodium bicarbonate aqueous solution. The mixture was then twice extracted with 30 ml of a mixed solvent of chloroform/methanol (5:1), and the extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain 301 mg of a crude preparation of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.11 (1H, m), 2.35 (1H, m), 2.43 (2H, m), 2.70 - 2.83 (4H, m), 2.90 (lH, m), 3.09 (1H, m), 4.30 (lH, t, J = 7.4 Hz), 6.65 (2H, d, J = 8.3 Hz), 6.88 (2H, d, J = 8.3 Hz), 7.62 (1K, dd, J = 7.3, 8.3 Hz), 7.64 (1H, t, J = 7.8 Hz), 8.17 (1H, d, J = 8.3 Hz), 8.24 - 8.31 (4H, m), 8.52 (lH, d, J = 5.9 Hz), 8.68 (lH, d, J = 6.3 Hz), 8.83 (1H, d, J = 6.4 Hz), 9.32 (lH, s), 9.43 (lH, s).
Example 29. l-Benzyloxycarbonyl-4-FN-(5-iso- cuinolinesulfonyl tyrosyl 1 piperazine 620 mg of the crude product obtained in Example 28 was dissolved in 10 ml of methylene chloride, and to the solution were sequentially added 0.29 ml of benzyloxycarbonyl chloride and 3.04 ml of triethylamine with ice cooling. After stirring for two hours with ice cooling, 40 ml of saturated sodium chloride aqueous solution was added to the reaction mixture, which was then extracted twice with 20 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain a residue.The residue was dissolved in 6 ml of methanol, and after the addition of 2 ml of 1 N sodium hydroxide aqueous solution, the mixture was refluxed for two hours, dried over magnesium sulfate and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (80:1 to 50:1) to obtain 336 mg of the title compound as colorless crystals.
Melting point: 137 - 1410C IR (RBr) cm : 1700, 1630, 1510, 1417, 1318, 1218, 1148, 1128; H-NMR(CDC13-CD30D, b ppm): 2.60 - 2.77 (2H, m), 2.80 - 3.55 (8H, m), 4.25 (1H, t, J = 7.8 Hz), 5.10 (1H, s), 5.12 (1H, s), 6.29, 6.74 (Total 2H, each d, each J = 8.3 Hz), 6.60, 7.01 (Total 2H, each d, each J = 8.3 Hz), 7.35 (5H, s), 7.60 (lH, t, J = 7.8 Hz), 8.15 (lH, d, J = 8.3 Hz), 8.26 (1H, d, J = 7.8 Hz), 8.29 (1H, d, J = 5.9 Hz), 8.57 (lH, d, J = 5.9 Hz), 9.25 (1H, s).
Example 30.
The same procedure as described in Example 29 was repeated to obtain l-[N-(5-isoquinolinesulfonyl)tyrosyl]-4-phenylacetylpiperazine in a yellow amorphous form.
IR (KBr) cm 1: 1620, 1510, 1435, 1320, 1228, 1152, 1130; H-NMR (DMSO-d6 , 6 ppm): 2.20 - 3.45 (10H, m), 3.67, 370 (Total 2H, each s), 4.32, 4.82 (Total 1H, each m), 6.45, 6.65 (Total 2H, each d, each J = 8.3 Hz), 6.82, 7.00 (Tctal 2H, each d, each J = 8.3 Hz), 7.13 7.39 (5K, m), 7.60 - 7.74 (1H, m), 8.13 - 8.42 (3H, m), 8.64 (1H, d, J = 5.9 Hz), 9.18 (1H, br), 9.39 (1H, br).
Example 31.
The same procedure as described in Example 29 was repeated to obtain 1-[N- ( 5-isoquinolinesulfonyl)- tyrosyl]-4-(3-phenylpropionyl)piperazine as colorless crystals.
Melting point: 172 - 1780C; IR (RBr) cm : 1630, 1510, 1440, 1320, 1225, 1150, 1128; 1H-NMR (CDC13-CD30D, 6 ppm): 2.50 - 3.47 (14H, m), 4.26 (lH, t, J = 7.3 Hz), 6.32 (2H, d, J = 8.3 Hz), 6.62 (2H, d, J = 8.3 Hz), 7.15 - 7.34 (5H, m), 7.62 (1H, t, J = 7.8 Hz), 8.17 (lH, d, J = 7.8 Hz), 8.24 - 8.33 (2H, m), 8.58 (1H, d, J = 5.4 Hz), 9.26 (1H, s).
Example 32. l-rN,O-bis(5-isoauinolinesulphonvll tyrosyll-4-(3-phenolpropvl)piperazine 301 mg of the crude product obtained in Example 28 and 95 mg of 3-phenylpropyl bromide were dissolved in 5 ml of dimethylformamide, and to the solution were added 66 mg of potassium carbonate and 72 mg of sodium iodide. After stirring at 800C for 7 hours, 30 ml of saturated sodium chloride was added to the reaction mixture, which was then extracted with 40 ml of ethyl acetate, and the extract was washed with 30 ml of saturated sodium chloride aqueous solution, dried over magnesium sulfate, and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (40:1) to obtain 216 mg of the title compound in a yellow amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.60 - 1.95 (6H, m), 2.06 - 2.29 (2H, m), 2.53 - 3.20 (8H, m), 4.28 (1H, m), 5.98 (1H, d, J = 9.3 Hz), 6.64 (2H, d, J = 8.3 Hz), 6.86 (2H, d, J = 8.3 Hz), 7.14 - 7.35 (5H, m), 7.59 (1H, t, J = 7.8 Hz), 7.62 (1H, t, J = 7.8 Hz), 8.12 (1H, d, J = 8.3 Hz), 8.23 - 8.29 (4H, m), 8.52 (1H, d, J = 5.9 Hz), 8.68 (1H, d, J = 6.4 Hz), 8.82 (lH, d, J = 6.4 Hz), 9.28 (lH, s), 9.42 (1H, s).
Example 33. 1-rN-(5-Isoquinolinesulfonvl) tyrosyl]-4-(3-phenylpropyl)piperazine 216 mg of the amorphous compound obtained in Example 32 was dissolved in 3 ml of methanol, and to the solution was added 0.6 ml of 2 N potassium hydroxide aqueous solution. The mixture was refluxed for 10 hours, and after the addition of 30 ml of saturated sodium chloride aqueous solution, extracted twice with 20 ml of a mixed solvent of chloroform/isopropanol (5:1). The extract was dried over magnesium sulfate and concentrated under a reduced pressure, and a resulting residue was applied to a silica gel column and eluted with chloroform/methanol (40:1 to 10:1) to obtain 74 mg of the title compound in a colorless amorphous form.
IR (KBr) cm : 1630, 1510, 1440, 1320, 1230, 1150, 1128; 1H-NMR (CDC13 , 6 ppm): 1.65 - 1.83 (2H, m), 2.00 - 2.37 (6H, m), 2.57 - 2.80 (4H, m), 3.02 - 3.42 (4H, m), 4.31 (lH, m), 6.30 (2H, d, J = 8.3 Hz), 6.41 (1H, d, J = 9.3 Hz), 6.65 (2H, d, J = 8.3 Hz), 7.15 - 7.37 (5H, m), 7.60 (lH, t, J = 7.8 Hz), 8.16 (1K, d, J = 8.3 Hz), 8.23 - 8.33 (2H, m), 8.58 (1H, br), 9.33 (1H, br).
Reference Example 12. l-N-(tert-Butoxycarbonyl) tyrosyl I -4-phenylpiperazine 19.7 g of N-(tert-butoxycarbonyl)tyrosine, 12.5 g of N-phenylpiperazine and 16.1 g of N-hydroxybenzotriazole were dissolved in 100 ml of tetrahydrofuran, and to the solution was added dropwise a solution of 18.7 g of DCC in 50 ml of tetrahydrofuran for 20 minutes with ice cooling, and the mixture was stirred for one hour. The reaction mixture was filtered to remove insoluble matter, which was then washed with 300 ml of ethyl acetate, and the filtrates were combined and concentrated under a reduced pressure.The resulting residue was dissolved in 500 ml of ethyl acetate, and the solution was washed three times with saturated sodium bicarbonate aqueous solution and once with saturated sodium chloride aqueous solution, dried over magnesium sulfate, and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with ethyl acetate/hexane (1:2 to 1:1) to collect desired fractions, which were then combined and concentrated under a reduced pressure.
Resulting residue was dissolved in 100 ml of ethyl acetate, and the solution was allowed to stand overnight in a refrigerator and then filtered to remove insoluble matter. The filtrate was concentrated under a reduced pressure, subjected to azeatropic distillation with benzene and dried under a reduced pressure to obtain 40.0 g of the title compound in a colorless amorphous form.
IR (KBr) cm : 1700, 1620, 1220; 1H-NMR (DMSO.d6 , 6 ppm): 1.33 (9H, s), 2.6 - 3.1 (6H, m), 3.4 - 3.7 (4H, m), 4.55 (1H, m), 6.64 2H, d, J = 8.2 Hz), 6.80 (1H, t, J = 7.3 Hz), 6.90 (2H, d, J = 7.9 Hz), 7.02 (2H, d, J = 8.2 Hz), 7.22 (2H, dd, J = 7.3, 7.9 Hz), 9.16 (lH, s).
Reference Example 13. 1-f2-ttert-Butoxycarbonsl- amino - 3 - (p-hvdroxyphenyl propyl 1-4 -phenylpipe- razine With ice cooling, to a solution of 8.0 g of lithium aluminum hydride in 230 ml of tetrahydrofuran a solution of 28.0 g of aluminum chloride in 230 ml of ether was added dropwise for 50 minutes, and after 15 minutes to the resulting solution was added dropwise a solution of 40.0 g of the amorphous compound obtained in Reference Example 12 in 230 ml of tetrahydrofuran, for 15 minutes.
The reaction mixture was allowed to become a room temperature, and after the addition of 300 ml of tetrahydrofuran, stirred for 25 minutes. The mixture was filtered to remove insoluble matter which was then washed with tetrahydrofuran. The combined filtrate was concentrated under a reduced pressure, and resulting residue was applied to a silica gel column, and eluted with chloroform/methanol (20:1) to collect fractions, which were then concentrated under a reduced pressure.
Then 100 ml of ethyl acetate was added to the residue to crystallize a product. The product was filtered to collect, and washed 5 times with a mother liquid and further 3 times with n-hexane and dried under a reduced pressure to obtain 24.1 g of the title compound as colorless crystals.
Melting point: 199 - 2020C (decomposed) H-NMR (DMSO-d6 , 6 ppm): 1.33 (9H, s), 2.2 - 2.8 (8H, m), 3.09 (4H, brs), 3.72 (lH, m), 6.5 - 7.0 (7H, m), 7.20 (2H, t, J = 8.3 Hz), 9.10 (1K, s); IR (KBr) cm 1690, 1500, 1230.
Reference Example 14. 1-F 2-Amino-3- (p-hydroxv- phenyl ) propyl 1 -4-phenylpiperazine To a suspension of 23.6 g of the crystals obtained in Reference Example 13 in 100 ml of ethyl acetate, was added dropwise 215 ml of 4N hydrochloric acid solution in ethyl acetate for 30 minutes, and after stirring for 90 minutes, excess hydrochloric acid was removed from the reaction mixture under a reduced pressure. After extraction with 200 ml of water, the separated ethyl acetate layer was extracted with 50 ml of 1 N hydrochloric acid aqueous solution. The aqueous layers were combined and neutralized to pH 7.4 with solid sodium bicarbonate, and resulting crystals was collected, washed with water and benzene and dried by phosphorus pentaoxide in a desiccator under a reduced pressure to obtain 16.9 g of the title compound as colorless crystals.
Melting point: > 2700C; IR (XBr) cm 1: 1600, 1470, 1230; 1H-NMR (DMSO-d6 , 6 ppm): 2.2 - 3.5 (13H, m), 6.7 - 6.8 (3H, m), 6.90 (2H, d, J = 8.3 Hz), 7.07 (2H, d, J = 8.3 Hz), 7.19 (2H, t, J = 7.6 Hz), 8.00 (2H, brs), 9.41 (1H, brs).
Example 34. N-1-Fp- (5-Isoouinolinesulfonyloxy) benzyl-2-(4-Phenylpiperazinyl)ethyl}-5-isoqui nolinesulfonamide To a suspension of 22.96 gof the crystals obtained in Reference Example 14 in 700 ml of tetrahydrofuran, was added 51.01 g of 5-isoquinolinesulfonyl chloride.HCl with ice cooling for 5 minutes, and then was added dropwise 103 ml of triethylamine for 30 minutes. After allowing to warm to a room temperature, the reaction mixture was poured into 460 ml of ice water, and the whole was extracted with 920 ml and 230 ml of chloroform. The combined extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated to dryness under a reduced pressure.The resulting amorphous residue was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1) to obtain 45.5 g of the title compound in a yellow amorphous form.
IR (RBr) cm 1 1600, 1500, 1130; 1H-NMR (CDC13 , 6 ppm): 2.0 - 3.0 (12H, m), 3.30 (lH, m), 5.51 (lH, brs), 6.7 - 7.8 (llH, m), 8.20 (lH, d, J = 8.2 Hz), 8.28 (2H, d, J = 7.7 Hz), 8.4 - 8.5 (2H, m), 8.53 (lH, d, J = 6.1 Hz), 8.67 (lH, d, J = 6.1 Hz) 8.81 (lH, d, J = 6.1 Hz), 9.35 (lH, s), 9.42 (lH, s).
Example 35. N-1-Fp-(5-Isouinolinesulfonyloxy) benzoll-2-(4-PhenYlPiserazinvl)etholi-N-methyl-5 isoquinolinesulfonamide To a solution of 25.0 g of the amorphous compound obtained in Example 34 in 200 ml of dimethylformamide was added in three portions 1.64 g of 60% sodium hydride, and after 5 minutes, also was added dropwise 3.14 ml of methyl iodide for two minutes, and the reaction mixture was stirred for one hour. The reaction mixture was poured to 400 ml of ice water, and the whole was extracted with 200 ml, 200 ml and 100 ml of ethyl acetate.The combined extract was washed three times with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure, and the resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 20.0 g of the tittle compound in a yellow amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.30 (lH, dd, J = 6.8, 12.2 Hz), 2.39 - 2.52 (5H, m), 2.68 (1H, dd, J = 7.3, 14.2 Hz), 2.86 (3H, s), 2.89 - 3.01 (5H, m), 4.18 (lH, m), 6.61 (2H, d, J = 8.3 Hz), 6.83 - 6.92 (5H, m), 7.26 (2H, t, J = 7.8 Hz), 7.57 (1K, t, J = 7.8 Hz), 7.60 (lH, t, J = 7.8 Hz), 8.10 (lH, d, J = 8.3 Hz), 8.23 - 8.28 (3H, m), 8.33 (lH, dd, J = 1.0, 7.3 Hz), 8.56 (lH, d, J = 5.9 Hz), 8.58 (lH, d, J = 5.9 Hz), 8.83 (lH, d, J = 5.9 Hz), 9.27 (lH, s), 9.41 (1H, d, J = 1.0 Hz) IR (RBr) cm 1 1620, 1500, 1370, 1325, 1130 Example 36. N-T1-(p-Hvdroxvbenzv1)-2-(4-phenvl- piDerazinyl!ethyll-N-methol-5-isoquinoline sulfonamide To 17.7 g of the amorphous compound obtained in Example 35 were added 240 ml of methanol, 60 ml of tetrahydrofuran and 29 ml of 2 N sodium hydroxide aqueous solution, and the mixture was refluxed for 150 minutes, and then poured to saturated sodium chloride aqueous solution. The mixture was extracted three times with 200 ml of chloroform, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate, and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (50:1), and 10.9 g of a yellow amorphous product was obtained from the elute.To the product was added 54 ml of ethanol and the mixture was stirred at a room temperature for one hour, and under ice cooling for 30 minutes to form crystals, which was then collected, washed three times with a mother liquid and twice with benzene, and dried under a reduced pressure to obtain 8.2 g of the title compound as light yellow crystals.
Melting point: 2010C; 1H-NMR (CDCl3 , 6 ppm): 2.49 (lH, dd, J = 6.8, 9.8 Hz), 2.52 - 2.77 (7H, m), 2.95 (1H, dd, J = 4.4, 14.2 Hz), 3.02 (3H, s), 3.14 (4H, t, J = 4.9 Hz), 4.03 (lH, m), 6.26 (2H, d, J = 8.3 Hz), 6.61 (2H, d, J = 8.8 Hz), 6.86 (1H, t, J = 6.8 Hz), 6.91 (2H, d, J = 7.3 Hz), 7.27 (2H, t, J = 7.8 Hz), 7.60 (1H, t, J = 7.3 Hz), 8.11 (lH, d, J = 5.9 Hz), 8.14 (lH, d, J = 6.4 Hz), 8.33 (lH, dd, J = 1.0, 7.3 Hz), 8.47 (lH, d, J = 6.3 Hz), 9.27 (1H, s); IR (KBr) cm : 1600, 1510, 1445, 1320, 1205, 1150, 1125.
Example 37.
The amorphous compound obtained in Example 34 was subjected to alkaline hydrolysis according to the procedure as described in Example 36, to obtain N-[l-(p-hydroxybenzyl)- 2-(4 -phenylpiperazinyl)ethyl]-5-- isoquinolinesulfonamide in a colorless amorphous.
1H-NMR (CDC13 , 6 ppm): 2.25 - 255 (6H, m), 2.65 (lH, dd, J = 13.7, 6.85 Hz), 2.79 (1H, dd, J = 13.7, 6.85 Hz), 2.82 - 3.0 (4H, m), 3.37 (1H, quintet, J = 6.85 Hz), 6.42 (2H, d, J = 8.57 Hz), 6.69 (2H, d, J = 8.57 Hz), 6.84 (2H, d, J = 8.57 Hz), 6.85 (1H, t, J = 8.57 Hz), 7.26 (2H, t, J = 8.57 Hz), 7.69 (1H, t, J = 7.42 Hz), 8.22 (1H, d, J = 7.99 Hz), 8.38 (1H, d, J = 6.28 Hz), 8.43 (lH, dd, J = 7.42, 1.0 Hz), 8.59 (1H, d, J = 6.28 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 38. N- F 1- (p-Methoxybenzyl -2- ( 4-phenyl- piperazinyl)ethyl]-N-methyl-5-isoquinoline- sulfonamide 1.51 g of the crystals obtained in Example 36 was dissolved in 20 ml of a mixed solvent of dimethyl formamide/tetrahydrofuran (1:1), and to the solution was added 140 mg of 60% sodium hydride with stirring under ice cooling, and stirring was continued for about 30 minutes. After foaming was finished, 490 mg of methyl iodide was added and the mixture was further stirred overnight at a room temperature. After the addition of ice, the reaction mixture was three times extracted with 50 ml of ethyl acetate, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure.The resulting residue was applied to a silicon gel column and eluted with chloroform/methanol (100:1) to obtain 1.55 g of the title compound as a light brown oil.
1H-NMR (CDC13 , S ppm): 2.45 (lH, dd, J = 7.1, 13.8 Hz), 2.6 (5H, m), 2.65 (1H, m), 2.88 (1H, s), 2.95 (3H, s), 3.05 (4H, m), 3.74 (3H, s), 4.2 (lH, m), 6.5 (2H, d, J = 8.5 Hz), 6.9 (5K, m), 7.25 (2H, m), 7.55 (lH, t, J = 7.5 Hz), 8.07 (lH, d, J = 7.5 Hz), 8.22 (1H, d, J = 6.4 Hz), 8.56 (lH, d, J = 6.4 Hz), 9.22 (lH, s); IR (KBr) cm 1 1600, 1510, 1320, 1240, 1150, 1130.
Example 39.
The same procedures as described in Reference Examples 12 to 14 and Example 34 were repeated except that l-(2-pyrimidyl)piperazine. dihydrochloride was used in place of N-phenylpiperazine, to obtain N-{1-[p-(5- isOquinolinesufonyloxy)benzyl]-2-[4-(2-pyrimidyl)pipera- zinyl]ethyl)-5-isoquinolinesulfonamide in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.8 - 1.96 (2H, m), 1.96 2.24 (4H, m), 2.8 (lH, dd, J = 13.7, 6.85 Hz), 2.92 (lH, dd, J = 13.7, 4.57 Hz), 3.0 - 3.47 (5H, m), 5.49 (lH, br), 6.47 (lH, t, J = 4.57 Hz), 6.70 (2H, d, J = 8.57 Hz), 6.94 (2H, d, J = 8.57 Hz), 7.64 (1H, t, J = 7.42 Hz), 7.70 (1H, t, J = 7.42 Hz), 8.17 - 8.35 (5H, m), 8.37 - 8.48 (2H, m) 8.52 (1H, d, J = 5.71 Hz), 8.68 (1H, d, J = 6.28 Hz), 8.82 (lH, d, J = 6.28 Hz) 9.37 (lH, s), 9.42 (lH, d, J = 1.0 Hz).
Example 40.
The amorphous compound of the Example 39 was treated as described in Example 37, to obtain N-{1-(p-hydroxybenzyl)-2-[4-(2-pyrimidyl)piperazi nylethyl)-5-isoquinolinesulfonamide in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 2.05 - 2.55 (6H, m), 2.66 (1H, dd, J = 13.13, 6.85 Hz), 2.82 (lH, dd, J = 13.13, 6.28 Hz), 3.2 - 3.7 (5H, m), 6.42 (2H, d, J = 7.99 Hz), 6.46 (lH, t, J = 4.57 Hz), 6.72 (2H, d, J = 7.99 Hz), 7.68 (1H, t, J = 7.42 Hz), 8.20 (lH, d, J = 8.57 Hz), 8.27 (2H, d, J = 4.57 Hz), 8.35 - 8.50 (2H, m), 8.57 (lH, d, J = 5.71 Hz), 9.31 (1H, s) Example 41.
The amorphous compound of Example 39 was treated as described in Example 35, to obtain N-{1-[p-(5-iso quinolinesulfonyloxy)benzyl]-2-(4-(2-pyrimidyl)piperazi- nyl]ethyl}-N-methyl-5-isoquinolinesulfonamide in a colorless amorphous form.
H-NMR (CDC13 , E ppm): 2.15 - 2.36 (5H, m), 2.44 (lH, dd, J = 13.7, 6.85 Hz), 2.71 (lH, dd, J = 1.3.13, 6.85 Hz), 2.8 - 2.95 (lH, m), 2.87 (3H, s), 3.56 (4H, m), 4.17 (1H, quintet, J = 6.85 Hz), 6.48 (lH, t, J = 4.85 Hz), 6.63 (2H, d, J = 9.14 Hz), 6.92 (2H, d, J = 9.14 Hz), 7.58 (1H, t, J = 6.85 Hz), 7.61 (1H, t, J = 6.85 Hz), 8.13 (1H, d, J = 7.42 Hz), 8.18 - 8.38 (6H, m), 8.56 (lH, d, J = 6.28 Hz), 8.58 (lH, d, J = 6.28 Hz), 8.84 (lH, d, J = 6.28 Hz), 9.28 (lH, s), 9.42 (1H, d, J = 1.0 Hz) Example 42.
The amorphous compound of Example 41 was treated as described in Example 36, to obtain N-{1-(p-hydroxy benzyl ) -2-[ 4-( 2-pyrimidyl )piperazinyl)ethyl}-N-methyl- 5-isoquinolinesulfonamide in a colorless amorphous form.
IR (KBr) cm : 1585, 1510, 1355, 1325, 1255, 1130; H-NMR (CDC13 , s ppm): 2.4 - 2.65 (6H, m), 2.70 (lH, dd, J = 13.13, 6.28 Hz), 2.97 (lH, dd, J = 13.13, 5.71 Hz), 3.03 (3H, s), 3.77 (4H, t, J = 4.57 Hz), 4.04 (lH, m), 6.28 (2H, d, J = 8.57 Hz), 6.49 (lH, t, J = 5.14 Hz), 6.62 (2H, d, J = 8.57 Hz), 7.62 (1H, t, J = 7.42 Hz), 8.11 (1H, d, J = 6.28 Hz), 8.15 (1H, d, J = 7.42 Hz), 8.30 (2H, d, J = 5.14 Hz), 8.32 (1H, dd, J = 7.42, 1.0 Hz), 8.48 (1H, d, J = 6.28 Hz), 9.28 (lH, s).
Example 43.
The same procedures as described in Reference Examples 12 to 14 and Examples 34 and 35 were repeated except that N- (tert-butoxycarbonyl ) phenylalanine was used in place of N-(tert-butoxycarbonyl) tyrosine, to obtain N-[1-benzyl-2-(4-phenylpiperazinyl)ethyl]-N- methyl-5-isoquinolinesulfonamide in a light yellow amorphous form.
IR (KBr) cm 1 1595, 1490, 1300, 1220, 1120; 1H-NMR (CDC13, 6 ppm): 2.45 (lH, dd, J = 6.6, 13 Hz), 2.7 (1H, dd, J = 8, 13 Hz), 2.55 (5H, m), 3.0 (5H, m), 4.3 (1H, m), 6.84, 6.9 (Total 3H, m), 7.0 (5H, brs), 7.25 (2H, m), 7.5 (1H, t, J = 7.5 Hz), 8.05 (1H, d, J = 8 Hz), 8.2 (1H, d, J = 7.5 Hz), 8.3 (1H, d, J = 8 Hz), 8.55 (1H, d, J = 6.1 Hz), 9.23 (1H, s).
Example 44.
The same procedures as described in Reference Examples 12 to 14 and Example 34 were repeated except that N-(2-pyridyl) piperazine was used in place of N-phenylpiperazine, to obtain N-(l-[p-(5-isoquinoline- sulfonyloxy)benzyl-2-[4-(2-pyridyl)piperazinyllethyl}-5isoquinolinesulfonamide in a yellow amorphous form IR (KBr) cm-1 : 1615, 1590, 1480, 1430, 1370, 1310, 1150, 1130; 1H-NMR (CDCl3 , 6 ppm): 1.93 - 2.21 (6H, m), 2.77 (1H, dd, J = 7.3, 14.2 Hz), 2.83 - 3.00 (3H, m), 3.02 3.19 (2H, m), 3.29 (lH, m), 5.46 (1H, br), 6.47 (1H, d, J = 8.8 Hz), 6.62 (lH, dd, J = 4.9, 7.3 Hz), 6.69 (2H, d, J = 8.8 Hz), 6.92 (2H, d, J = 8.8 Hz), 7.44 (1H, ddd, J = 1.0, 8.8, 7.3 Hz), 7.64 (1H, t, J = 7.8 Hz), 7.70 (1H, dd, J = 7.3, 8.3 Hz), 8.13 (lH, dd, J = 1.0, 4.9 Hz), 8.22 (1H, d, J = 8.3 Hz), 8.28 (2H, d, J = 7.3 Hz), 8.43 (2H, m), 8.53 (1H, d, J = 5.9 Hz), 8.67 (1H, d, J = 6.3 Hz), 8.81 (lH, d, J = 5.9 Hz), 9.35 (1H, d, J = 1.0 Hz), 9.42 (1H, s).
Example 45.
The product of Example 44 was treated as described in Example 35 to obtain N-{1-[p-(5-isoquinoline- sulfonyloxy)benzyl]-2-[4-(2-pyridyl)piperazinyl]ethyl} -N-methyl-5-isoquinolinesulfonamide IR (KBr) cm-1 : 1590, 1480, 1430, 1370, 1310, 1130; 1H-NMR (CDCl3 , 6 ppm): 2.23 - 2.50 (6H, m), 2.69 (1H, dd, J = 7.3, 14.2 Hz), 2.86 (3H, s), 2.88 (1H, dd, J = 14.2, 10.2 Hz), 3.30 (4H, m), 4.18 (1H, m), 6.55 6.65 (4H, m), 6.90 (2H, d, J = 8.8 Hz), 7.47 (1H, ddd, J = 1.0, 7.3, 8.8 Hz), 7.58 (1H, dd, J = 7.3, 8.3 Hz), 7.60 (lH, t, J = 7.8 Hz), 8.11 (1H, d, J = 8.3 Hz), 8.17 (1H, dd, J = 1.0, 4.9 Hz), 8.22 - 8.27 (3H, m), 8.33 (lH, dd, J = 1.0, 7.3 Hz), 8.56 (1H, d, J = 5.9 Hz), 8.58 (lH, d, J = 5.9 Hz), 8.84 (1H, d, J = 6.4 Hz), 9.28 (lH, s), 9.41 (lH, s).
Example 46.
The product of Example 45 was treated as described in Example 36 to obtain N-{1-(p-hydroxybenzyl)2-[ 4- ( 2-pyridyl )piperazinyl]ethyl}-N-methyl-5-isoquino- linesulfonamide.
IR (RBr) cm : 1590, 1475, 1445, 1320, 1230, 1150, 1125; H-NMR (CDCl3 , 6 ppm): 2.48 (1H, dd, J = 3.4, 9.4 Hz), 2.50 - 2.75 (6H, m), 2.95 (1H, dd, J = 4.9, 14.7 Hz), 3.02 (3H, s), 3.49 (4H, t, J = 4.9 Hz), 4.06 (lH, m), 6.27 (2H, d, J = 8.3 Hz), 6.62 (2H, d, J = 8.3 Hz), 6.61 - 6.66 (2H, m), 7.43 (1H, ddd, J = 1.0, 7.3, 8.8 Hz), 7.61 (lH, dd, J = 7.3, 8.3 Hz), 8.10 8.16 (2H, m), 8.19 (1H, dd, J = 1.0, 4.3 Hz), 8.32 (1H, dd, J = 1.0, 7.3 Hz), 8.48 (1H, d, J = 6.4 Hz), 9.28 (lH, s).
Example 47.
The same procedures as described in Reference Example 12 to 14 and Examples 34 to 36 were repeated except that N-(m-chlorophenyl)piperazine was used in place of N-phenylpiperazine, to obtain N-{2-[4-(m chlorophenyl)piperazinyl]-l-(p-hydroxybenzyl)ethyl]-N methyl-5-isoquinolinesulfonamide in a light yellow amorphous form.
IR (KBr) cm 1 1590, 1320, 1230, 1130; 1H-NMR (CDC13 , s ppm): 2.5 (1H, dd, J = 12.0, 10 Hz), 2.5 - 2.8 (2H, m), 2.6 - 2.7 (4H, m), 2.95 (lH, dd, J = 4.5, 13.8 Hz), 3.0 (3H, s), 3.15 (4H, m), 4.0 (1H, m), 6.22 (2H, d, J = 8.0 Hz), 6.55 (2H, d, J = 8.0 Hz), 6.77 (1H, dd, J = 8.5, 2.2 Hz), 6.8 (lH, d, J = 8.0 Hz), 6.85 (1H, d, J = 2.2 Hz), 7.16 (lH, t, J = 8.0 Hz), 7.6 (1H, t, J = 7.8 Hz), 8.1 (lH, d, J = 6.1 Hz), 8.15 (1H, d, J = 8.1 Hz), 8.3 (1H, d, J = 7.3 Hz), 8.45 (lH, d, J = 6.4 Hz), 9.28 (lH, s).
Example 48.
The same procedures as described in Reference Examples 12 to 14 and Example 34 were repeated except that N-(p-fluorophenyl)piperazine was used in place of N-phenylpiperazine, to obtain N-(2-[4-(p-fluoro- phenyl)piperazinyl]-l-[p-(5-isoquinolinesulfonylOxy)ben- zyl)ethyl] }-S-isoquinolinesulfonamide in a colorless amorphous form.
IR (KBr) cm 1 1610, 1500, 1370, 1320, 1210, 1130, 860, 820; 1H-NMR (CDC13 , 6 ppm): 2.0 - 2.3 (5H, m), 2.4 2.9 (6H, m), 3.3 (1H, m), 6.6 - 6.75 (4H, m), 6.85 - 7.0 (4H, m), 7.65 (1H, t, J = 8.1 Hz), 7.7 (1H, t, J = 8.4 Hz), 8.2 (1H, d, J = 8.3 Hz), 8.3 (lH, d, J = 7.8 Hz), 8.4 (1H, d, J = 6.3 Hz), 8.4 (lH, d, J = 6.1 Hz), 8.5 (lH, d, J = 6.1), 8.65 (1H, d, J = 6.1 Hz), 8.8 (1H, d, J = 6.3 Hz), 9.3 (1H, s), 9.4 (1H, s).
Example 49.
The amorphous compound obtained in Example 48 was methylated according to the procedure described in Example 35 to obtain N-{2-[4-(p-fluorophenyl) piperazinyl]-l-[p-(5-isoquinolinesulfonyloxy)ben- zyl]ethyl]}-N-methyl-5-isoquinolinesulfonamide in a light yellow amorphous form.
IR (KBr) cam : 1620, 1510, 1370, 1330, 1210, 1140 1H-NMR (CDC13 , 6 ppm): 2.3 (1H, dd, J = 12.1, 6.5 Hz), 24.5 (4H, m), 2.4 - 2.6 (lH, m), 2.67 (1H, dd, J = 13.8, 7.8 Hz), 2.75 - 3.0 (5H, m), 4.17 (1H, m), 6.63 (2H, d, J = 8.6 Hz), 6.7 - 7.0 (6H, m), 7.57 (1H, t, J = 8.0 Hz), 7.60 (1H, t, J = 7.6 Hz), 8.1 (1H, d, J = 8.0 Hz), 8.2 - 8.35 (4H, m), 8.55 (1H, d, J = 5.4 Hz), 8.56 (1H, d, J = 8.1 Hz), 8.83 (1H, d, J = 6.3 Hz), 9.27 (lH, d, J = 0.7 Hz), 9.40 (1H, d, J = 1.0 Hz) Example 50.
According to the procedure described in Example 36, 160 mg of the amorphous compound obtained in Example 49 was dissolved in 2 ml of methanol, and to the solution was added 0.5 ml of 2N sodium hydroxide, and the reaction mixture was refluxed for two hours, cooled, and extracted three times with chloroform. The extract was purified on a silica gel column using chloroform/methanol (100:2), to obtain 103 mg of N-(l-(p- hydroxybenzyl)-2-[4-(p-fluorophenyl)piperazinyl]ethyl} N-methyl-5-isoquinolinesulfonamide in a light yellow amorphous form.
IR (KBr) cm 1 1610, 1500, 1320, 1230, 1150, 1130, 820; 1H-NMR (CDC13 , s ppm): 2.4 - 2.6 (2H, m), 2.6 2.8 (1H, m), 2.8 - 3.0 (1H, m), 2.75 (4H, m), 3.05 (1H, m), 3.1 (1H, m), 6.3 (2H, d, J = 8.3 Hz), 6.67 (2H, d, J = 8.3 Hz), 6.87 (2H, dd, J = 8.3, 10.1 Hz), 6.95 (2H, t, J = 8.3 Hz), 7.6 (1H, dd, J = 7.6, 8.0 Hz), 8.12 (1H, d, J = 9.0 Hz), 8.13 (1H, d, J = 6.1 Hz), 8.3 (1H, d, J = 7.3 Hz), 8.5 (1H, d, J = 6.1 Hz), 9.25 (1H, s).
Example 51.
The same procedures as described in Reference Examples 12 to 14 and Examples 34 to 36 were repeated except that N-(m-methylphenyl)piperazine was used in place of N-phenylpiperazine, to obtain N-(l-(p-hydroxy- benzyl)-2-[4-(m-methylphenyl)piperazinyl]ethyl}-N- methyl-5-isoquinolinesulfonamide in a light yellow amorphous form.
IR (KBr) cm 1 1600, 1440, 1320, 1210, 1190, 1150, 1120; 1H-NMR (CDC13 , 6 ppm): 2.30 (3H, s), 2.55 (4H, m), 2.96 (lH, dd, J = 11.6, 7.1 Hz), 2.5 - 2.9 (3H, m), 2.9 (3H, s), 3.1 .(4H, m), 4.3 (1H, m), 6.8 (2H, d, J = 8.3 Hz), 7.0 (2H, d, J = 8.3 Hz), 7.0 - 7.15 (3H, m), 7.3 (1H, m), 7.55 (1H, t, J = 7.8 Hz), 8.1 (lH, d, J = 7.8 Hz), 8.2 - 8.3 (2H, complex), 8.58 (1H, d, J = 6.1 Hz), 9.25 (1H, s).
Example 52.
The same procedures as described in reference Examples 12 to 14 and Example 34 were sequentially repeated except that N-(p-methoxyphenyl)piperazine was used in place of N-phenylpiperazine, to obtain N-{l-[p-(5-isoquinolinesulfonyloxy)benzyl-2-[4-(p-methoxyphenyl)piperazinyl]ethyl}-5-isoquinolinesulfonamide in yellow amorphous form.
IR (KBr) cm 1 1615, 1500, 1360,'1130; 1H-NMR (CDC13 , 6 ppm): 1.96 - 2.22 (6H, m), 2.39 - 2.52 (2H, m), 2.52 - 2.67 (2H, m), 2.77 (lH, dd, J = 7.3, 14.2 Hz), 2.90 (1H, dd, J = 4.4, 14.2 Hz), 3.27 (1H, m), 3.76 (3H, s), 5.50 (1H, br), 6.70 (4H, d, J = 8.8 Hz), 6.82 (2H, d, J = 8.8 Hz), 6.94 (2H, d, J = 8.8 Hz), 7.65 (1H, t, J = 7.8 Hz), 7.70 (1H, t, J = 7.3 Hz), 8.21 (1H, d, J = 8.3 Hz), 8.29 (2H, d, J = 7.8 Hz), 8.40 - 8.43 (2H, m), 8.53 (1H, d, J = 5.9 Hz), 8.68 (1H, d, J = 6.4 Hz), 8.81 (1H, d, J = 5.9 Hz), 9.36 (1H, s), 9.42 (1H, s).
Example 53.
The amorphous compound of Example 52 was treated with methyl iodide according to the procedure described in Example 35 to obtain N-{1-[p-(5-iso- quinolinesulfonyloxy)benzyl]-2-[4-(p-methoxyphenyl)piperazinyl]ethyl}-N-methyl-5-isoquinolinesulfonamide in a yellow amorphous form.
IR (KBr) cm : 1665, 1615, 1505, 1365, 1320, 1130; H-NMR (CDCl3 , 6 ppm): 2.30 (1H, dd, J = 6.8, 12.2 Hz), 2.37 - 2.51 (SH, m), 2.68 (lH, dd, J = 7.3, 14.2 Hz), 2.85 (3H, s), 2.78 - 2.97 (5H, m), 3.77 (3H, s), 4.16 (1H, m), 6.62 (2H, d, J = 8.3 Hz), 6.82 (4H, s), 6.90 (2H, d, J = 8.3 Hz), 7.57 (1H, t, J = 7.8 Hz), 7.60 (1H, t, J = 7.8 Hz), 8.11 (1H, d, J = 8.3 Hz), 8.23 - 8.28 (3H, m), 8.33 (1H, dd, J = 1.0, 7.3 Hz), 8.56 (1H, d, J = 6.4 Hz), 8.58 (1H, d, J = 6.4 Hz), 8.83 (1H, d, J = 5.9 Hz), 9.27 (lH, d, J = 1.0 Hz), 9.41 (1H, d, J = 1.0 Hz).
Example 54.
The amorphous compound obtained in Example 53 was subjected to alkaline hydrolysis according to the procedure described in Example 36 to obtain N {1-(p-hydroxybenzyl)-2-[4-(p-methoxyphenyl)piperazinyl]- ethyl)-N-methyl-5-isoquinolinesulfonamid as yellow crystals, Melting point: 157 - 1600C (decomposed); IR (KBr) cm 1 1615, 1510, 1445, 1320, 1305, 1240 1125; 1H-NMR (CDCl3 , 6 ppm): 2.46 - 2.74 (7H, m), 2.88 - 3.02 (5H, m), 3.00 (3H, s), 3.77 (3H, s), 4.06 (1H, m), 6.30 (2H, d, J = 8.3 Hz), 6.65 (2H, d, J = 8.3 Hz), 6.83 (2H, d, J = 9.3 Hz), 6.88 (2H, d, J = 9.3 Hz), 7.57 (lH, dd, J = 7.3, 8.3 Hz), 8.12 (lH, d, J = 8.3 Hz), 8.13 (lH, d, J = 6.4 Hz), 8.32 (lH, dd, J = 1.0, 7.3 Hz), 8.50 (1H, d, J = 6.4 Hz), 9.26 (1H, s).
Example 55.
The amorphous compound obtained in Example 52 was subjected to alkaline hydrolysis according to the procedure described in Example 37, to obtain N-{1-(p-hydroxybenzyl)-2-[4- (p-methoxyphenyl)pipera- zinyl]ethyl)-5-isoquinolinesulfonamide as yellow crystals.
Melting point: 200 - 2080C (decomposed); IR (KBr) cm 1 1615, 1590, 1510, 1450, 1340, 1230, 1150, 1130, 1025; 1H-NMR (CDC13 , 6 ppm): 2.20 - 2.44 (6H, m), 2.58 - 2.82 (6H, m), 3.33 (1H, m), 3.77 (3H, s), 5.55 (1H, br), 6.47 (2H, d, J = 8.3 Hz), 6.76 (2H, d, J = 8.3 Hz), 6.78 (2H, d, J = 6.8 Hz), 6.83 (2H, d, J = 6.8 Hz), 7.70 (1H, t, J = 7.8 Hz), 8.21 (1H, d, J = 8.3 Hz), 8.40 (1H, d, J = 6.4 Hz), 8.44 (1H, dd, J = 1.0, 7.3 Hz), 8.64 (1H, d, J = 6.4 Hz), 9.34 (1H, s).
Example 56.
The same procedures as described in Reference Examples 12 to 14 and Examples 34 and 35 were sequentially repeated except that N-(2-methoxyphenyl)piperazine was used in place of N-phenylpiperazine to obtain N-{1-[p-(S-isoquinolinesulfonyloxy)benzyl)-2-[4-(2- methOxyphenyl)piperazinyl]ethyl}-N-methyl-5-isOqui- nolinesulfonamide, and 800 mg of the compound was subjected to alkaline hydrolysis according to the procedure described in Example 36 to obtain 504 mg of N-{1-(p-hydroxybenzyl)-2-[4-(2-methoxyphenyl)pipe razinyl]ethyl}-N-methyl-5-isoquinolinesulfonamide in a light yellow amorphous form.
IR (KBr) cm 1 1610, 1590, 1500, 1320, 1230, 1130; 1H-NMR (CDC13 , 6 ppm): 2.5 (1H, dd, J = 13.8, 10.0 Hz), 2.55 - 2.8 (2H, m), 2.9 - 3.0 (1H, m), 2.7 (4H, m), 3.0 (4H, m), 3.05 (3H, s), 3.86 (3H, s), 4.0 (1H, m), 6.23 (2H, d, J = 8.3 Hz), 6.57 (2H, d, J = 8.3 Hz), 6.8 - 7.1 (4H, m), 7.6 (1H, t, J = 8.0 Hz), 8.14 (1H, d, J = 6.1 Hz), 8.16 (1H, d, J = 7.9 Hz), 8.35 (1H, dd, J = 7.4, 1.0 Hz), 9.30 (1H, s).
Example 57.
1-[2-Amino-3-(p-hydroxyphenyl)propyl]-4-phenyl- piperazine in crystals obtained in Reference Example 14 was reacted with 1-naphtharenesulfonyl chloride according to the procedure of Example 34, and the product thus obtained was treated with methyl iodide according to the procedure described in Example 35, to obtain N-methyl-N-{1-[p-(&alpha;-naphtharenesulfonyloxy) -benzyl]-2-(4-phenylpiperazinyl)ethyl}-&alpha;- naphtharenesulfonamide in colorless amorphous form.
H-NMR (CDCl3 , 6 ppm): 2.26 (1H, dd, J = 12.56, 6.85 Hz), 2.42 (4H,m), 2.48 (lH, dd, J = 12.56, 6.85 Hz), 2.68 (1H, dd, J = 14.85, 6.85 Hz), 2.84 (1H, dd, J = 14.85, 6.85 Hz), 2.85 (3H, s), 2.92 (4H,m), 4.12 (1H, quintet, J = 6.85 Hz), 6.56 (2H, d, J = 8.0 Hz), 6.78 - 6.92 (5H, m), 7.26 (2H, t, J = 8.0 Hz), 7.42 (2H, t, J = 8.0 Hz), 7.46 - 7.58 (2H, m), 7.68 (1H, dt, J = 8.0, 1.0 Hz), 7.77 - 7.90 (2H, m), 7.90 - 8.07 (3H, m), 8.12 (1H, d, J = 8.57 Hz), 8.17 (1H, dd, J = 8.57, 1.0 Hz), 8.45 (lH, m), 8.84 (1H, d, J = 9.14 Hz) Example 58 The amorphous compound obtained in Example 57 was subjected to alkaline hydrolysis according to the procedure described in Example 36, to obtain N-[1-(p-hydroxybenzyl)-2-(4-phenylpiperazinyl)ethyl]- N-methyl-a-naphtharenesulfonamide in a colorless amorphous form.
IR (KBr) cm 1 1595, 1315, 1310,1225, 1150, 1120; H-NMR (CDCl3 , 6 ppm): 2.37 (1H, dd, J = 13.70, 6.85 Hz), 2.47 (4H, m), 2.55 (1H, dd, J = 13.70, 6.85 Hz), 2.72 (1H, dd, J = 14.28, 6.85 Hz), 2.85 (lR, dd, J = 14.28, 6.85 Hz), 2.89 (3H, s), 2.97 (4H, m), 4.27 (1H, quinted, J = 6.85 Hz), 5.10 (1H, br), 6.57 (2H, d, J = 7.99 Hz), 6.78 - 6.89 (3H, m), 6.91 (2H, d, J = 7.99 Hz), 7.25 (2H, t, J = 7.99 Hz), 7.43 (lH, t, J = 7.42 Hz), 7.50 - 7.63 (2H, m), 7.86 (1H, dd, J = 7.99, 1.0 Hz), 7.98 (lH, d, J = 7.42 Hz), 8.22 (1H, dd, J = 7.42, 1.0 Hz), 8.56 (1H, dd, J = 7.99, 1.0 Hz).
Reference Example 15. 1-{[2-Amino-3-(p-hydroxy)- phenyl]propyl}-4-benzyloxycarbonylpiperazine 1.41 g of 1- (N-tert-butoxycarbonyltyrosyl)-4- (benzyloxycarbonyl)piperazine prepared according to the procedure described in Reference Example 11 was dissolved in 5.6 ml of absolute ethyl acetate, and to the solution was added dropwise 11.25 ml of 4N hydrogen chloride in ethyl acetate with stirring under ice cooling for two minutes, and the reaction mixture was stirred for 2 hours at a room temperature. After the reaction was completed, the solvent was evaporated off under a reduced pressure, and to the residue was added 20 ml of 5% sodium bicarbonate aqueous solution.The mixture was extracted with 30 ml and then 20 ml of a mixed solvent of chloroform/methanol (9:1), and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and filtrated.
The filtrate was evaporated under a reduced pressure to obtain a colorless foam. The foam was applied to a silica gel column and eluted with chloroform/methanol (6:1), to obtain about 700 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 2.2 - 2.8 (8H, m), 3.19 (1H, m), 3.50 (7H, brs), 5.13 (2H, s), 6.71 (2H, d, J = 8.5 Hz), 7.02 (2H, d, J = 8.5 Hz), 7.35 (5H, s).
Example 59. N-2-(4-Benzyloxycarbonylpipera- zinvl!-l-rp-(5-isoquinolinesulfonoloxvlbenzYll- ethyli-5-isoauinolinesulfonamide 680 mg of the amorphous compound obtained in Reference Example 15 was dissolved in 18 ml of absolute tetrahydrofuran, and to the solution was added 1.27 g of 5-isoquinolinesulfonyl chloride.HCl, and then was added dropwise 3.20 ml of triethylamine with stirring under ice cooling for one minute, and the mixture was stirred at a room temperature for 150 minutes. The reaction mixture was diluted with 50 ml of chloroform, washed with water, and the washings was extracted with 20 ml of chloroform. The combined organic extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and filtered, and the filtrate was evaporated under a reduced pressure.The resulting residue was applied to a silica gel column, and eluted with chloroform/methanol (100:1) to obtain 987 mg of the title compound in a light yellow amorphous form.
IR (KBr) cm : 1700, 1620, 1500, 1370, 1230, 1130; H-NMR (CDC13 , 6 ppm): 1.8 - 2.2 (6H, m), 2.7 3.4 (7H, m), 5.05 (2H, s), 5.36(1H, brs), 6,67 (2H, d, J = 8.4 Hz), 6.89 (2H, d, J = 8.4 Hz), 7.2 - 7.4 (5H, m), 7.62 (1H, d, J = 7.7 Hz), 7.69 (lH, d, J = 7.7 Hz), 8.2 - 8.5 (5H, brs), 8.52 (1H, d, J = 6.2 Hz), 8.68 (1H, d, J = 6.2 Hz), 8.81 (1H, d, J = 5.9 Hz), 9.34 (1H, s), 9.41 (1H, s).
Example 60. N-{2-(4-Benzyloxycarbonylpipera- zinyl -1- F P- (S-isopuinolinesulfonyloxy)benzyl 1 - ethl-N-methvl-5-isosuinolinesulfonamid 852 mg of the amorphous compound obtained in Example 59 was dissolved in 8.5 ml of absolute dimethylformamide, and to the solution was added 59 mg of 60% sodium hydride with stirring under ice cooling, and further was added 113 p1 of methyl iodide, and the reaction mixture was stirred for 2 hours with ice cooling. After the addition of 30 ml of ice water1 the reaction mixture was extracted with 30'ml, 20 ml and 20 ml of ethyl acetate, the combined extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate, filtered and concentrated under a reduced pressure.The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 679 mg of the title compound in a light yellow amorphous form.
IR (KBr) cm : 1700, 1620, 1500, 1370, 1240, 1130; H-NMR (CDCl3 , 6 ppm): 2.23 (5H, brs), 2.3 - 2.9 (3H, m), 2.83 (3H, s), 3.25 (4H, brs), 4.09 (1H, m), 5.11 (2H, s), 6.60 (2H, d, J = 8.7 Hz), 6.86 (2H, d, J = 8.7 Hz), 7.34 (5H, brs), 7.5 - 7.7 (2H, m), 8.12 (lH, d, J = 8.2 Hz), 8.1 - 8.3 (4H, m), 8.56 (2H, m), 8.84 (lH, d, J = 6.1 Hz), 9.29 (lH, s), 9.41 (1H, s).
Example 61. N-f2- [(4-Benzenesulfonyl)pipera- (4-Benzenesulfonyl )pipera- zinyl 1-1-[p-( 5-isocruinolinesulfonyloxy benzyl]- ethyl}-N-methyl-5-isoguinolinesulfonamid To 480 mg of the amorphous compound obtained in Example 60 was added 3 ml of a solution of 30% hydrogen bromide in acetic acid, and the mixture was stirred at a room temperature for 80 minutes. Then 50 ml of ether was added to the mixture, which was then stirred. The resulting precipitate was collected and washed with ether and dried under a reduced pressure to obtain 567 mg of N-{1-[p-(5-isoquinolinesulfonyloxy)benzyl] -2-piperazinyl-ethyl)-N-methyl-5 -isoquinolinesulfonamide.HBr as colorless crystals.
H-NMR (DMSO - d6 + D2O, 6 ppm): 3.06 (3H, s), 3.46 (12H, brs), 4.24 (lH, brs), 6.08 (2H, d, J = 8.5 Hz), 6.74 (2H, d, J = 8.5 Hz), 7.93 (2H, m), 8.25 (1H, d, J = 6.7 Hz), 8.35 (1H, d, J = 7.3 Hz), 8.5 - 8.8 (5H, m), 9.05 (lH, d, J = 6.4 Hz), 9.86 (2H, brs).
550 mg of the crystals thus obtained was suspended in 10 ml of absolute tetrahydrofuran, and after stirring with ice cooling, to the suspension were added 84 1 of benzenesulfonyl chloride and 767 p1 of triethylamine, and the reaction mixture was stirred at a room temperature for 140 minutes. Then 30 ml of chloroform and 20 ml of water were added to the reaction mixture, and after separation of the layers, the aqueous layer was extracted with 20 ml of chloroform. The chloroform layers were combined, washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate, and filtered. The filtrate was evaporated under a reduced pressure, and the resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 331 mg of the title compound in a light yellow amorphous form.
IR (KBr) cm : 1620, 1500, 1440, 1330, 1170; H-NMR (CDC13 , 6 ppm): 2.1 - 2.5 (6H, m), 2.5 2.8 (6H, m), 2.78 (3H, s), 4.05 (1H, m), 6.60 (2H, d, J = 8.7 Hz), 6.83 (2H, d, J = 8.7 Hz), 7.37 (lH, t, J = 7.8 Hz), 7.5 - 7.8 (6H, m), 7.98 (lH, d, J = 7.9 Hz), 8.1 - 8.3 (4H, m), 8.49 (lH, d, J = 6.1 Hz), 8.55 (lH, d, J = 6.1 Hz), 8.84 (1H, d, J = 6.1 Hz), 9.17 (1H, s), 9.42 (1H, s) Example 62.N- 2- F ( 4-Benzenesulfonyl}pipera- (4-Benzenesulfonyl pipera- zinyl)-1-(p-hydroxybenzyl-ethyl-N-methyl- 5-isoquinolinesulfonamide 221 mg of the amorphous compound obtained in Example 61 was dissolved in a mixed solution of 2.69 ml of methanol and 0.66 ml of tetrahydrofuran, and to the solution was added 0.33 ml of 2N sodium hydroxide aqueous solution. The reaction mixture was refluxed for 3.5 hours, and to the mixture were added 30 ml of chloroform and 20'ml of 10% ammonium chloride aqueous solution, and the resulting layers were separated. The aqueous layer was extracted with 20 ml of chloroform, and the chloroform layers were combined, washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and filtered.The filtrate was evaporated under a reduced pressure, and the resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 146 mg of the title compound in a colorless amorphous form.
IR (KBr) cm : 1620, 1510, 1440, 1320, 1160; 1H-NMR (CDC13 , 6 ppm): 2.3 - 2.5 (2H, m), 2.60 (4H, brs), 2.6 - 2.8 (2H, m), 2.95 (3H, s), 3.01 (4H, brs), 3.92 (lH, brs), 6.21 (2H, d, J = 8.2 Hz), 6.51 (2H, d, J = 8.2 Hz), 7.4 - 7.8 (6H, m), 8.03 (lH, d, J = 6.1 Hz), 8.09 (lH, d, J = 8.2 Hz), 8.24 (lH, dd, J = 1.2, 7.3 Hz), 8.40 (lH, d, J = 5.8 Hz), 8.68 (lH, brs), 9.23 (1H, brs).
Reference Example 16. O-Benzvl-N-benzYloxy- carbonyltyrosinol 27. 25 g of O-benzyl-N-benzyloxycarbonyltyrosine methyl ester was dissolved in a mixed solvent of 185 ml of ethanol and 122 ml of tetrahydrofuran, and to the solution were added 5.8 g of lithium chloride and 5.2 g of sodium borohydride under ice cooling. The reaction mixture was stirred at a room temperature for 18 hours, and after the addition of 500 ml of saturated sodium chloride aqueous solution, extracted twice with 300 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure to obtain 25.4 g of the title compound as colorless crystals.
1H-NMR (CDC13 , 6 ppm): 2.79 (2H, d, J = 7.4 Hz), 3.51 - 3.79 (2H, m), 3.89 (lH, m), 4.93 (lH, br), 5.04 (2H, s), 5.08 (2H, s), 6.90 (2H, d, J = 8.6 Hz), 7.11 (2H, d, J = 8.6 Hz), 7.26 - 7.46 (5H, m).
Reference Example 17. 1-F2-Benzyloxycarbonylamino- 3- (p-benzyloxyphenyl propyl 1-4-( tert-butoxycar- bonyl !piperazine 5.6 g of the crystals obtained in Reference Example 16 was dissolved in 70 ml of carbon tetrachloride, and after the addition of 4.5 g of triphenylphosphine, the mixture was refluxed for 20 hours. The reaction mixture was filtered to remove insoluble matter, and the filtrate was concentrated under a reduced pressure, and the resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (6:1) to obtain 4.96 g of 2-benzyloxycarbonylamino-3-(p-benzyloxyp chloride as colorless crystals.
1H-NMR (CDCl3 , 6 ppm): 2.76 - 2.90 (2H, m), 3.56 - 3.80 (2H, m), 4.39 (lH, m), 5.03 (2H, s), 5.05, 5.13 (Total 2H, each s), 6.85, 6.89 (Total 2H, each d, each J = 8.3 Hz), 7.00, 7.09 (Total 2H, each d, each J = 8.3 Hz), 7.23 - 7.45 (5H, m).
4.1 g of the above-crystals and 2.23 g of N- (tert-butoxycarbonyl )piperazine were dissolved in 40 ml of dimethylformamide, to the solution were added 1.8 g of methyl iodide and 1.66 g of potassium car bonate, and the mixture was stirred at 1200C for 3 hours. After the addition of 100 ml of saturated sodium chloride aqueous solution, the reaction mixture was extracted twice with 60 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (2:1) to obtain 2.69 g of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , S ppm): 1.45 (9H, s), 2.22 - 2.49 (6H, m), 2.82 (2H, m), 3.36 (4H, m), 3.94 (1H, m), 4.83 (lH, br), 8.03 (2H, s), 5.09 (2H, s), 6.88 (2H, d, J = 8.3 Hz), 7.06 (2H, d, J = 8.3 Hz), 7.30 - 7.45 (5H, m).
Example 63. N-; 2-F 4- (tert-Butoxycarbonyl pipera- zinvll-l- r P- 5-isoquinolinesulfonsloxy!benzoll ethl}-5-isosquinolinesulfonamide 1.6 g of the amorphous compound obtained in Reference Example 17 was dissolved in 25 ml of methanol, and to the solution was added 1.0 g of 5% palladium on carbon. The mixture was stirred in a hydrogen atmosphere for 20 hours, and filtered to remove insoluble matter. The filtrate was concentrated under a reduced pressure, resulting residue was dissolved in 30 ml of tetrahydrofuran, and to the solution were added 2.8 g of 5-isoquinolinesulfonyl chloride.HCl and 4 ml of triethylamine under ice cooling. After stirring at a room temperature for 3 hours, to the reaction mixture was added 100 ml of water, and the mixture was extracted twice with 70 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1) to obtain 1.27 g of the title compound in a yellow amorphous form.
1H-NMR (CDCl3 , s ppm): 1.40 (9H, s), 1.75 - 2.18 (6K, m), 2.15 - 3.07 (6H, m), 3.27 (1H, m), 5.35 (1H, br), 6.67 (2H, d, J = 8.3 Hz), 6.90 (2H, d, J = 8.3 Hz), 7.65 (lH, t, J = 7.8 Hz), 7.69 (lH, t, J = 7.8 Hz), 8.21 (lH, d, J = 8.3 Hz), 8.27 - 8.32 (2H, m), 8.37 - 8.41 (2H, m), 8.53 (lH, d, J = 6.4 Hz), 8.69 (lH, d, J = 6.9 Hz), 8.82 (1H, d, J = 6.4 Hz), 9.36 (lH, s), 9.43 (1K, s).
Example 64.
940 mg of the amorphous compound obtained in Example 63 was dissolved in a mixed solvent of 7.5 ml of tetrahydrofuran and 7.5 ml of dimethylformamide, and to the solution were sequential added 67 mg of 60% sodium hydride and 0.11 ml of methyl iodide under ice cooling, and the mixture was stirred at a room temperature for one hour. After the addition of 30 ml of saturated sodium chloride aqueous solution, the reaction mixture was extracted with 40 ml of ethyl acetate, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure.Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (60:1) to obtain 723 mg of N-C2-[4-(tert-butoxy- carbonyl)piperazinyl]-[p-(5-isoquinolinesulfonylOxy)ben- zyl]ethyl}-N-methyl-S-isoquinolinesulf6namide in a yellow amorphous form.
H-NMR (CDC13 , s ppm): 1.44 (9H, s), 2.21 (5H, m), 2.40 (lH, dd, J = 6.9, 12.6 Hz), 2.15 (lH, dd, J = 7.4, 14.3 Hz), 2.83 (lH, dd, J = 6.9, 14.3 Hz), 2.84 (3H, s), 3.17 (9H, m), 4.12 (1H, m), 6.60 (2H, d, J = 8.8 Hz), 6.86 (2H, d, J = 8.8 Hz), 7.58 (1K, t, J = 7.8 Hz), 7.63 (1H, t, J = 7.8 Hz), 8.13 (1H, d, J = 8.3 Hz), 8.21 - 8.30 (4H, m), 8.56 (1K, d, J = 6.9 Hz), 8.57 (1H, d, J = 5.9 Hz), 8.84 (1H, d, J = 5.9 Hz), 9.29 (1H, s), 9.42 (1H, s).
Example 65.
To 720 mg of the amorphous compound obtained in Example 64 was added 10 ml of 4N hydrochloric acid in ethyl acetate, and the mixture was stirred at a room temperature for one hour and concentrated under a reduced pressure. To the mixture was added 30 ml of saturated sodium bicarbonate aqueous solution, and the reaction mixture was extracted twice with 20 ml of a mixed solvent of chloroform/isopropanol (5:1). The extract was dried over magnesium sulfate and concentrated to dryness under a reduced pressure to obtain 620 mg of N-(l-[p-(5-isoquinolinesulfonyloxy)ben- zyl]ethyl-2-piperazinyl}-N-methyl-5-isoquinolinesulfonamide in a yellow amorphous form.
1H-NMR (CDC13 , 6 ppm): 2.18 - 2.28 (5H, m), 2.37 (lH, dd, J = 7.3, 12.7 Hz), 2.63 (4H, m), 2.66 (1H, dd, J = 7.3, 14.8 Hz), 2.83 (3H, s), 2.86 (1H, dd, J = 5.4, 14.8 Hz), 4.13 (lH, m), 6.61 (2H, d, J = 8.8 Hz), 6.89 (2H, d, J = 8.8 Hz), 7.59 (lH, t, J = 7.8 Hz), 7.63 (1H, t, J = 7.8 Hz), 8.12 (1K, d, J = 8.3 Hz), 8.23 - 8.35 (4H, m), 8.56 (1H, d, J = 5.9 Hz), 8.58 (1H, d, J = 6.4 Hz), 8.83 (1H, d, J = 6.9 Hz), 9.29 (1K, s), 9.42 (lH, s).
Example 66.
620 mg of the amorphous compound obtained in Example 65 was dissolved in 10 ml of methylene chloride, to the solution were added 0.29 ml of benzyl chloroformate and 0.4 ml of triethylamine with ice cooling.
The reaction mixture was stirred for two hours with ice cooling, and after the addition of 40 ml of saturated sodium chloride aqueous solution, extracted twice with 20 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (60:1) to obtain 660 mg of light yellow amorphous product showing the same 1H-NMR spectrum as that of the compound of Example 60.
Example 67.
650 mg of the amorphous compound obtained in Example 66 was dissolved in 6 ml of methanol, and to the solution was added 2 ml of 1 N sodium hydroxide aqueous solution. The mixture was refluxed for 2 hours, and after the addition of 30 ml of saturated sodium chloride aqueous solution, extracted twice with 200 ml of a mixed solvent of chloroform/isopropanol (5:1).The extract was dried over magnesium sulfate and concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (80:1 to 50:1) to obtain 386 mg of N-[2-(4-benzyloxycarbonyl piperazinyl)-1-(p-hydroxybenzyl)ethyl]-N-methyl-5-iso- quinolinesulfonamide in a yellow amorphous form IR (KBr) cm : 1693, 1510, 1425, 1320, 1235, 1120; 1H-NMR (CDCl3 , 6 ppm): 2.39 - 2.55 (6H, m), 2.64 (1H, dd, J = 6.4, 12.7 Hz), 2.87 (1H, dd, J = 4.4, 14.6 Hz), 2.97 (3H, s), 3.44 (4H, m), 4.03 (1H, m), 5.13 (2H, s), 6.28 (2H, d, J = 8.3 Hz), 6.62 (2H, d, J = 8.3 Hz), 7.36 (5H, s), 7.39 (1H, dd, J = 7.3, 7.8 Hz), 8.09 (1H, d, J = 5.9 Hz), 8.13 (1H, d, J = 7.8 Hz), 8.26 (1H, d, J = 7.3 Hz), 8.48 (1X, d, J = 5.9 Hz), 9.27 (1H, s).
Example 68.
In the amorphous compound obtained in Example 63, protecting group of the piperazine moiety was removed according to the procedure described in Example 65, and the deprotected intermediate was treated according to the procedures described in Examples 66 and 67 to obtain N-[2-(4-benzyloxycarbonylpiperazinyl)-1-(p-hydroxybenzyl)ethyl]-5-isoquinolinesulfonamide.
IR (KBr) cm 1 1670, 1510, 1425, 1320, 1230, 1150, 1125, 993; H-NMR (CDC13 , 6 ppm): 2.07 - 2.23 (4H, m), 2.30 (1H, dd, J = 6.3, 13.2 Hz), 2.39 (1K, dd, J = 7.8, 13.2 Hz), 2.62 (1H, dd, J = 6.8, 14.2 Hz), 2.76 (1K, dd, J = 6.4, 14.2 Hz), 3.00 - 3.38 (5H, m), 5.09 (2H, s), 6.41 (2H, d, J = 8.3 Hz), 6.69 (2H, d, J = 8.8 Hz), 7.33 (5H, s), 7.68 (1H, dd, J = 7.3, 8.3 Hz) 8.21 (1H, d, J = 8.3 Hz), 8.35 (lH, d, J = 5.9 Hz), 8.40 (lH, dd, J =1.0, 7.3 Hz), 8.61 (lH, d, J = 5.9 Hz), 9.34 (1H, s).
Example 69.
The same procedure as described in Example 68 was repeated except that phenylpropionyl chloride was used in place of benzyl chloroformate, to obtain N-(l-(p- hydroxybenzyl)-2-[4-(3-phenylpropyl)piperazi- nyl]ethyl)-5-isoquinolinesulfonamide.
IR (KBr) cm 1 1610, 1510, 1445, 1320, 1150, 1130, 993; H-NMR (CDC13 , 6 ppm): 2.00 - 2.20 (4H, m), 2.26 (1H, dd, J = 5.9, 12.7 Hz), 2.36 (lH, dd, J = 7.8, 12.7 Hz), 2.46 - 2.60 (2H, m), 2.62 - 2.77 (2H, m) 2.83 - 3.00 (2H, m), 3.00 - 3.41 (5H, m), 5.24 (lH, br), 6.43 (2H, d, J = 8.3 Hz), 6.71 (2H, d, J = 8.3 Hz), 7.13 7.34 (5H, m), 7.69 (lH, dd, J = 7.3, 8.3 Hz), 8.20 (1H, d, J = 7.8 Hz), 8.34 (lH, d, J = 5.9 Hz), 8.40 (lH, dd, J = 1.0, 7.3 Hz), 8.63 (1H, br), 9.12 (1H, br).
Example 70.
The same procedure as described in Example 68 was repeated except that phenylisocyanate was used in place of benzyl chloroformate, to obtain N-[1-(p-hydroxy benzyl)-2-(4-phenylaminocarbonylpiperazinyl)ethyl]-5- isoquinolinesulfonamide in yellow amorphous form.
1H-NMR (CDC13 + CD3OD, 6 ppm): 2.37 (1H, dd, J = 8.8, 14.2 Hz), 2.65 (lH, dd, J = 5.4, 13.7 Hz), 2.73 3.20 (4H, m), 3.40 - 3.95 (7H, m), 6.12 (2H, d, J = 8.3 Hz), 6.50 (2H, d, J = 8.3 Hz), 7.05 (lH, m), 7.25 7.38 (4H, m), 7.67 (lH, dd, J = 7.8, 8.3 Hz), 8.21 (lH, d, J = 7.8 Hz), 8.31 (1H, d, J = 6.8 Hz), 8.53 (1H, br), 9.23 (1H, br).
Example 71.
The same procedure as described in Example 68 was repeated except that benzylisocyanate was used in place of benzyl chloroformate, to obtain N-[2-(4-benzylaminocarbonylpiperazinyl)-1-(p-hydroxybenzyl)ethyl] 5-isoquinolinesulfonamide in a yellow amorphous form.
1H-NMR (CDC13 - CD3OD, s ppm): 2.40 - 2.71 (2H, m), 2.76 - 3.23 (4H, m), 3.40 - 3.80 (7H, m), 4.39 (2H, s), 6.08 (2H, d, J = 8.3 Hz), 6.46 (2H, d, J = 8.3 Hz), 7.20 - 7.38 (5H, m), 7.63 (lH, t, J = 7.8 Hz), 8.17 (1H, d, J = 8.3 Hz), 8.29 (2H, d, J = 6.9 Hz), 8.52 (lH, br), 9.23 (lah, br).
Example 72.
200 mg of the amorphous compound obtained in Example 67 was dissolved in 5 ml of chloroform, and to the solution was added 1 ml of methanol, and also a solution of diazomethane in ether with ice cooling. The reaction mixture was stirred for 90 minutes and then concentrated. Resulting residue was applied to silica gel column and eluted with chloroform/methanol (80:1 to 50:1), to obtain 103 mg of N-[2-(4-benzyloxycarbonyl piperazinyl)-1-(p-methoxybenzyl)ethyl]-N-methyl- 5-isoquinolinesulfonamide.
IR (KBr) cm 1 1692, 1508, 1420, 1320, 1235, 1120; H-NMR (CDC13 , 6 ppm): 2.32 - 2.45 (5H, m), 2.53 - 2.65 (2H, m), 2.78 - 2.90 (1K, m) 2.92 (3H, s), 3.37 (4H, m), 3.73 (3H, s), 4.16 (1H, m), 5.11 (2H, s), 6.50 (2H, d, J = 8.3 Hz) 6.82 (2H, d, J = 8.3 Hz), 7.35 (5K, s), 7.55 (1H, t, J = 7.8 Hz), 8.09 (lH, d, J = 8.3 Hz), 8.18 (lH, d, J = 6.4 Hz), 8.22 (1H, d, J = 7.3 Hz), 8.55 (1H, d, J = 6.4 Hz), 9.24 (1H, s).
Example 73.
The product of Example 68 was treated according to the procedure in Example 72, to obtain N-[2-(4 benzylOxyCarbonylpiperazinyl)-l-(p-methoxybenzyl)ethyl]- 5-isoquinolinesulfonamide.
H-NMR (CDC13 , 6 ppm): 1.97 - 2.17 (4H, m), 2.19 (1H, dd, J = 4.4, 13.7 Hz), 2.28 (lH, dd, J = 6.8, 13.7 Hz), 2.70 (1H, dd, J = 6.8, 14.2 Hz), 2.82 (lH, dd, J = 5.4, 14.2 Hz), 3.03 (2H, m), 3.15 (2H, m), 3.34 (1H, m), 3.74 (3H, s), 5.07 (2H, s), 5.36 (1H, br), 6.59 (2H, d, J = 8.8 Hz), 6.85 (2H, d, J = 8.3 Hz), 7.27 - 7.37 (5H, m), 7.68 (lH, dd, J = 7.3, 7.8 Hz), 8.18 (1H, d, J = 8.3 Hz), 8.39 (1H, d, J = 5.9 Hz), 8.42 (1H, dd, J = 1.0, 7.8 Hz), 8.67 (1H, d, J = 5.9 Hz), 9.33 (1H, s).
Example 74. N-;2- (4-Benzoylpiperazinyl-1- [p-(5-isoquinolinesulfonyloxy)benzyl]ethyl}-N methvl-5-isosuinolinesulfonamide 764 mg of the intermediate crystals obtained in Example 61 was suspended in 7 ml of tetrahydrofuran, to the suspension were added 135 mg of benzoyl chloride and 5 minutes later 1.1 ml of triethylamine with ice cooling, and the mixture was stirred for one hour.
After adding chloroform and water, the reaction mixture was extracted three times with 30 ml of chloroform, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated to dryness under a reduced pressure.
0.82 g of the resulting pale yellow residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 198 mg of the title compound in a colorless amorphous form.
IR (RBr) cm 1 1620, 1370, 1130; 1H-NMR (CDCl3 , 6 ppm): 2.2 - 2.8 (10H, m)., 2.84 (3H, s), 3.1 - 3.7 (4H, m), 4.14 (1H, m), 6.61 (2H, d, J = 8.6 Hz), 6.85 (2H, d, J = 8.6 Hz), 7.3 - 8.6 (14K, m), 8.84 (lH, d, J = 5.8 Hz), 9.30 (lH, s), 9.43 (1H, s).
Example 75. N- r 2- (4-Benzoylpiperazinyl -1- (p-hydroxybenzyl)ethyl]-N-methyl-5-isoquinoline- sulfonamide To 349 mg of the amorphous compound obtained in Example 74 were added 4 ml of methanol, 1 ml of tetrahydrofuran and 0.5 ml of 2 N sodium hydroxide, and after ref fluxing for 2 hours, to the reaction mixture were added chloroform, water and sodium chloride. The reaction mixture was three times extracted with 30 ml of chloroform, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure to obtain 0.25 g of yellow oil. The yellow oil was applied to a silica gel column and eluted with chloroform/methanol (50:1) to obtain 145 mg of the title compound in a colorless amorphous form.
IR (KBr) cm 1 1610, 1440, 1120; 1H-NMR (CDCl3 r 6 ppm): 2.6 - 2.9 (8H, m), 2.99 (3H, s), 3.2 - 3.9 (4H, m), 4.00 (1H, m), 6.25 (2H, d, J = 8.5 Hz), 6.56 (2H, d, J = 8.5 Hz), 7.41 (5H, s), 7.59 (1R, t, J = 7.9 Hz), 8.08 (1H, d, J = 6.1 Hz), 8.15 (1H, d, J = 7.9 Hz), 8.25 (1H, d, J = 7.9 Hz), 8.46 (1H, d, J = 6.1 Hz), 9.29 (1H, s) Example 76.
The same procedures as described in Examples 74 and 75 were sequentially repeated except that 470 mg of the intermediate crystals in Example 61 and 113 mg of benzylsulfonyl chloride in place of benzoyl chloride were used to obtain 116 mg of N-[2-(4-benzylsulfonyl piperazinyl)-l-(p-hydroxybenzyl)ethyl]-N-methyl-5- isoquinolinesulfonamide in a colorless amorphous form.
IR (RBr) cm 1 1610, 1320, 1150; 1H-NMR (CDC13 , 6 ppm): 2.3 - 2.9 (8H, m), 2.94 (3H, s), 3.0 - 3.2 (4H, m), 4.03 (lH, m), 4.20 (2H, s), 6.26 (2H, d, J = 8.2 Hz), 6.56 (2H, d, J = 8.2 Hz), 7.40 (5H, s), 7.60 (1H, t, J = 7.9 Hz), 8.07 (1H, d, J = 6.4 Hz), 8.15 (1H, d, J = 7.9 Hz), 8.22 (1H, d, J = 7.9 Hz), 8.46 (1H, d, J = 6.4 Hz), 9.29 (1H, s).
Example 77.
The same procedures as described in Examples 74 and 75 were sequentially repeated except that 382 mg of the intermediate crystals in Example 61 and 133 mg of 5-isoquinolinesulfonamide*HCl in place of benzoyl chloride were used, to obtain N-{1-(p-hydroxybenzyl]-2-[4-(5-isoquinolinesulfonyl)piperazinyl)ethyl} N-methyl-5-isoquinolinesulfonamide in a colorless amorphous form.
IR (RBr) cm : 1610, 1320, 1150; K-NMR (CDC13 , 6 ppm): 2.3 - 2.8 (8H, m), 2.90 (3H, s), 3.0 - 3.2 (4H, m), 3.95 (lH, m), 6.29 (2H, d, J = 8.6 Hz), 6.53 (2H, d, J = 8.6 Hz), 7.50 (1H, t, J = 7.9 Hz), 7.75 (1H, t, J = 7.9 Hz), 8.0 - 8.1 (2H, m), 8.17 (1H, d, J = 7.9 Hz), 8.26 (1H, d, J = 7.9 Hz), 8.3 - 8.5 (2H, m), 8.53 (1H, d, J = 6.1 Hz), 8.70 (1H, d, J = 6.1 Hz), 9.19 (1H, s), 9.39 (1H, s).
Example 78.
The same procedures as described in Examples 59 and 60 were sequentially repeated except that a-naphtharenesulfonyl chloride was used in place of 5-isoquinolinesulfonyl chloride.HC1, to obtain N-{2-(4 benzyloxycarbonylpiperazinyl)-1-[p-( &alpha;-naphtharene- sulfonylOxy)benzyl]ethyl}-N-methyl-a-naphtharenesulfon- amide in a colorless amorphous form.
IR (KBr) cm : 1700, 1365, 1130, 860, 765; H-NMR (CDCl3 , 6 ppm): 2.05 - 2.25 (5H, m), 2.41 (1H, dd, J = 13.13, 6.85 Hz), 2.55 - 2.8 (2H, m), 2.84 (3H, s), 3.05 - 3.25 (4H, m), 4.05 (1H, quintet, J = 6.85 Hz), 5.11 (2H, s), 6.57 (2H, d, J = 8.57 Hz), 6.82 (2H, d, J = 8.57 Hz), 7.25 - 7.60 (9H, m), 7.60 - 8.20 (8H, m), 8.42 (1H, m), 8.82 (1H, d, J = 7.99 Hz).
Example 79.
The amorphous compound obtained in Example 78 was subjected to alkaline hydrolysis according to the procedure in Example 62 to obtain N-[2-(4-benzyloxy carbonylpiperazinyl)-1-(p-hydroxybenzyl)ethyla-N-methyl -a-naphtharenesulfonamide in a colorless amorphous form.
IR (KBr) cm : 1695, 1670, 1310, 1240, 1120; 1H-NMR (CDC13 , E ppm): 2.15 - 2.4 (5H, m), 2.48 (lH, dd, J = 12.56, 7.42 Hz), 2.6 - 2.85 (2H, m), 2.87 (3H, s), 3.15 - 3.35 (4H, m), 4.20 (1H, quintet, J = 6.85 Hz), 5.10 (2H, s), 5.13 (1H, br), 6.58 (2H, d, J = 7.99 Hz), 6.89 (2H, d, J = 7.99 Hz), 7.33 (5H, s), 7.44 (1H, t, J = 7.42 Hz), 7.47 - 7.63 (2H, m), 7.8 - 7.93 (1H, m), 7.99 (1H, d, J = 7.99 Hz), 8.15 (1H, dd, J = 6.85, 1.0 Hz), 8.45 - 8.6 (1H, m).
Example 80. N-f1- r P- ( 5-Isoquinolinesulf onylOxv ! benzyl-2-(4-phenylhomopiperazinyl)ethyl}-5-iso- auinolinesulfonamide The same procedure as described in Example 34 except that 1-[2-amino-3-(p-hydroxyphenyl)propyl]-4- phenylhomopiperazine was used in place of 1-[2-amino3-(p-hydroxyphenyl )propyl]-4-phenylpiperazine, to obtain the title compound in a yellow amorphous form.
IR (KBr) cam : 1620, 1600, 1365, 1135; 1H-NMR (CDC13 , 6 ppm): 2.10 - 2.36 (7H, m), 2.63 (lH, dd, J = 6.8, 13.7 Hz), 2.76 (1H, dd, J = 4.9, 13.7 Hz), 2.99 - 3.29 (6H, m), 6.53 (2H, d, J = 8.3 Hz), 6.60 (2H, d, J = 8.8 Hz), 6.64 (1H, t, J = 7.3 Hz), 6.80 (2H, d, J = 8.8 Hz), 7.17 (1H, t, J = 8.8 Hz), 7.62 (1H, t, J = 7.8 Hz), 7.66 (1H, t, J = 7.8 Hz), 8.18 (1H, d, J = 8.3 Hz), 8.26 (2H, d, J = 7.3 Hz), 8.37 - 8.38 (2H, m), 8.53 (1H, d, J = 5.9 Hz), 8.67 (1H, d, J = 6.4 Hz), 8.82 (1H, d, J = 5.9 Hz), 9.33 (1H, s), 9.42 (1H, s).
Example 81.
The amorphous compound obtained in Example 80 was treated with methyl iodide according to the procedure in Example 35 to obtain N-{1-[p-(5-iso- quinolinesulfonyloxy)benzyl ] -2- (4-phenylhomopiperazi- nyl)ethyl}-N-methyl-5-isoquinolinesulfonamide in a yellow amorphous form.
IR (KBr) cm : 1620, 1600, 1500, 1365, 1135; 1H-NMR (CDC13 , E ppm): 1.70 (2H, m), 2.37 (1H, dd, J = 8.8, 11.7 Hz), 2.40 - 2.65 (6H, m), 2.76 (1H, dd, J = 4.9, 13.7 Hz), 2.84 (3H, s), 3.23 - 3.42 (4H, m), 3.98 (1H, m), 6.51 - 6.65 (5H, m), 6.77 (2H, d, J = 8.3 Hz), 7.16 (2H, t, J = 7.8 Hz), 7.54 (1H, dd, J = 7.3, 8.3 Hz), 7.59 (1H, t, J = 7.8 Hz), 8.08 (1H, d, J = 7.8 Hz), 8.18 - 8.26 (4H, m), 8.55 (1H, d, J = 5.9 Hz), 8.55 (1H, dd, J = 1.0, 6.3 Hz), 8.84 (1H, d, J = 6.3 Hz), 9.26 (1H, s), 9.41 (1H, d, J = 1.0 Hz).
The amorphous compounds obtained in Examples 80 and 81 were subjected to alkaline hydrolysis according to the procedure described in Example 36, to obtain the following two compounds.
Example 82. N-[1-(p-Hydroxybenzyl-2-(4-phenyl- homopiperazinyl)ethyl]-5-isoquinolinesulfonamide Yellow crystals; Melting point: 170 - 1780C (decomposed); IR (KBr) cm : 1615, 1600, 1505, 1365, 1320, 1205, 1155, 1130; 1H-NMR (CDC13 , 6 ppm): 1.79 (2H, m), 2.44 (1H, dd, J = 8.3, 13.7 Hz), 2.50 - 2.72 (7H, m), 3.23 (1H, m), 3.30 - 3.45 (4H, m), 6.24 (2H, d, J = 8.3 Hz), 6.51 (2H, d, J = 8.3 Hz), 6.66 (2H, d, J = 8.3 Hz), 6.68 (1H, t, J = 7.3 Hz), 7.23 (2H, dd, J = 7.3, 8.3 Hz), 7.64 (1H, t, J = 7.8 Hz), 8.19 (1H, d, J = 7.8 Hz), 8.27 (1H, d, J = 6.4 Hz), 8.34 (1H, dd, J = 1.0, 7.3 Hz), 8.53 (1H, d, J = 6.4 Hz), 9.33 (1H, brs).
Example 83. N- [1-(p-Hydroxybenzyl)-2-(4-phenyl- homopiperazinyl)ethyl]-N-methyl-5-isoquinoline- sulfonamide Yellow amorphous.
IR (KBr) cm : 1615, 1600, 1500, 1360, 1320, 1210, 1150, 1125; 1H-NMR (CDC13 , 6 ppm): 1.93 (2H, m), 2.40 (1H, dd, J = 9.8, 14.2 Hz), 2.56 (lH, dd, J = 8.8, 12.7 Hz), 2.70 (2H, m), 2.80 - 2.92 (4H, m), 3.00 (3H, s), 3.46 3.53 (4H, m), 3.85 (1H, m), 6.19 (2H, d, J = 8.3 Hz), 6.51 (2H, d, J = 8.3 Hz), 6.65 (1H, t, J = 7.3 Hz), 6.69 (2H, d, J = 8.3 Hz), 7.21 (2H, dd, J = 7.3, 8.8 Hz), 7.58 (2H, t, J = 7.3 Hz), 8.07 (1H, d, J = 6.4 Hz), 8.12 (1H, d, J = 8.3 Hz), 8.27 (lH, dd, J = 1.0, 7.3 Hz), 8.45 (1H, d, J = 5.9 Hz), 9.27 (1H, s) Reference Example 18. N-tert-ButoxYcarbonvl-4- hydroxypiperidine 7.14 g of 4-piperidone.monohydrate.hydrochloride was dissolved in 50 ml of dimethylformamide and 10 ml of water, and to the solution were added 25 ml of diisopropylethylamine and 9.5 g of di-tert-butyl dicarbonate at a room temperature with stirring, and the reaction mixture was stirred for 4 hours. After adding water and saturating with sodium chloride, the reaction mixture was extracted twice with 300 ml of chloroform. The extract was dried over magnesium sulfate and evaporated under a reduced pressure to obtain 9.6 g of residue, which was then dissolved in 100 ml of ethanol.To the solution was added 1.83 g of sodium borohydride with stirring under ice cooling, and the mixture was stirred for 90 minutes under the same condition, and then for 30 minutes at a room temperature. After adding saturated sodium chloride aqueous solution, the reaction mixture was alkalized with sodium bicarbonate and extracted twice with 600 ml of chloroform. The extract was dried over magnesium sulfate and evaporated under a reduced pressure, and resulting residue was subjected to a silica gel column and eluted with hexane/ethyl acetate (2:1), to obtain 8.03 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.35 - 1.6 (2H, m), 1.45 (9H, s), 1.75 - 1.95 (2H, m), 3.03 (2H, ddd, J = 13.13, 10.28, 4.00 Hz), 3.75 - 3.95 (3H, m).
Reference Example 19. 4-(p-Methylbenzvloxy) piperidine 2.0 g of the amorphous compound obtained in Reference Example 18 was dissolved in 20 ml of dimethylformamide, to the solution was added 0.48 g of 60% sodium hydride in an ice bath. After removing from the ice bath, the reaction mixture was stirred at a room temperature for 30 minutes, and after adding 1.54 g of p-methylbenzyl chloride, further stirred for 18 hours.
The reaction mixture was poured on ice, saturated with sodium chloride and extracted twice with 150 ml of chloroform. The extract was dried over magnesium sulfate and evaporated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (5:1) to obtain 1.27 g of N-tert-butoxycarbonyl-4-p-methylbenzyloxypiperidine.
This compound was dissolved in 3 ml of ethyl acetate, and after adding 12 ml of 3N hydrogen chloride in ethyl acetate at a room temperature with further stirring for 17 hours, the solvent was evaporated off under a reduced pressure. Resulting residue was dissolved in water, and the solution was alkalized with sodium bicarbonate, saturated with sodium chloride and extracted twice with 200 ml of chloroform. The extract was dried over magnesium sulfate and the solvent was evaporated off under a reduced pressure to obtain 830 mg of the title compound in colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.55 - 1.8 C2R, m), 1.9 2.15 (2H, m), 2.34 (3H, s), 2.81 (2H, ddd, J = 13.13, 10.28, 4.00 Hz), 3.17 (2H, ddd, J = 11.42, 7.42, 4.00 Hz), 3.56 (1H, septet, J = 3.71 Hz), 4.50 (2H, s), 5.01 (1H, brs), 7.14 (2H, d, J = 7.99 Hz), 7.22 (2H, d, J = 7.99 Hz).
Reference Example 20. N-tert-Butoxycarbonyl-o-(2- methoxyethoxymethvl )tyrosine methyl ester 13.39 g of N-tert-butoxycarbonyltyrosine methyl ester was dissolved in 65 ml of tetrahydrofuran and 65 ml of dimethylformamide, and to the solution was added 1.9 g of 60% sodium hydride with stirring in an ice bath. After removing from the ice bath, the mixture was stirred at a room temperature for 30 minutes, and after addition of 5.4 g of methoxyethoxymethyl chloride with ice cooling, stirred for 15 hours allowing to warm to a room temperature. The reaction mixture was poured on ice, saturated with sodium chloride and extracted twice with 800 ml of chloroform.The extract was dried over magnesium sulfate and the solvent was evaporated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (4:1) to obtain 13.85 g of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , s ppm): 1.46 (9H, s), 3.02 (2H, m), 3.37 (3H, s), 3.5 - 3.6 (2H, m), 3.71 (3H, s), 3.75 - 3.85 (2H, m), 4.54 (1H, m), 4.95 (lH, m), 5.24 (2H, s), 6.96 (2H, d, J = 9.71 Hz), 7.04 (2H, d, J = 9.71 Hz).
Reference Example 21. 2- (N-tert-Butoxycarbonyl- amino ! -l-chloro-3- r p- ( 2 -methoxvethoxvmethoxv ) phe nyl 1 propane 13.85 g of the amorphous compound obtained in Reference Example 20 was dissolved in 90 ml of ethanol and 60 ml of tetrahydrofuran, and to the solution were added 3.11 g of lithium chloride and 2.77 g of sodium borohydride with stirring in an ice bath. After removing from the ice bath, the mixture was stirred at a room temperature for 16 hours, and after addition of satulated sodium chloride aqueous solution, the reaction mixture was alkalized with sodium bicarbonate and extracted twice with 800 ml of chloroform.The extract was dried over magnesium sulfate and the solvent was evaporated off under a reduced pressure, to obtain 11.73 g of N-tert-butoxycarbonyl-o-(2methoxyethoxymethyl)tyrosinol. This compound was dissolved in 120 ml of of carbon tetrachloride, and to the solution was added 10 g of triphenylphosphine. The mixture was refluxed for 3 hours and further heated at 800C for 17 hours. The solvent was evaporated off under a reduced pressure, and the resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) followed by with hexane/ethyl acetate (4:1), to obtain 7.22 g of the title compound in colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.43 (9H, s), 2.75 2.9 (2H, m), 3.38 (3H, s), 3.4 - 3.65 (4H, m), 3.75 3.9 (2H, m), 4.08 (1H, m), 4.79 (1H, m), 5.26 (2H, s), 6.99 (2H, d, J = 9.71 Hz), 7.16 (2H, d, J = 9.71 Hz).
Reference Example 22. N- 2- (tert-Butoxycarbonyl- amino)-3- [p-(2-methoxyethoxymethoxy)phenyl propyl}-4-(p-methylbenzyloxy)piperidine 1.56 g of the amorphous compound obtained in Reference Example 21 was dissolved in 25 ml of dimethylformamide, to the solution were added 0.83 g of the amorphous compound obtained in Reference Example 19, 0.67 g of potassium carbonate and 0.67 g of sodium iodide, and the mixture was stirred at 1000C for 2 hours, and after adding saturated sodium chloride aqueous solution, extracted twice with 150 ml of chloroform. The extract was dried over magnesium sulfate and the solvent was evaporated off under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 490 mg of the title compound in a colorless amorphous form.
H-NMR (CDCl3 , s ppm): 1.42 (9H, s), 1.5 - 1.75 (3H, m), 1.75 - 2.0 (2H, m), 2.0 - 2.3 (3H, m), 2.33 (3H, s), 2.55 - 2.95 (4H, m), 3.25 - 3.5 (lH, m), 3.38 (3H, s), 3.5 - 3.65 (2H, m), 3.75 - 3.95 (3H, m), 4.49 (2H, s), 4.64 (1H, m), 5.25 (2H, s), 6.96 (2H, d, J = 9.71 Hz), 7.09 (2K, d, J = 9.71 Hz), 7.14 (2H, d, J = 9.71 Hz), 9.23 (2H, d, J = 9.71 Hz).
Example 84. N-f1- r p- ( S-Isopuinolinesulfonyloxy) benzvll-2- r 4-(D-methvlbenzoloxv!PiPeridinolethylE- 5-isoquinolinesulfonamide 490 mg of the amorphous compound obtained in Reference Example 22 was dissolved in 1 ml of ethyl acetate, and to the solution was added 5 ml of 3 N hydrogen chloride in ethyl acetate at a room temperature with stirring, and the reaction mixture was stirred for one-hour. After evaporating off the solvent, resulting residue was alkalized with sodium bicarbonate aqueous solution, and the mixture was saturated with sodium chloride, washed with a small amount of methanol, and extracted twice with 100 ml of chloroform.The extract was dried over magnesium sulfate, and the solvent was evaporated off to obtain a residue comprising N- [ 2-amino-3- (p-hydroxyphenyl) ]propyl-4- (p- methylbenzyloxy)piperidine. The residue was dissolved in 7 ml of tetrahydrofuran, and to the solution were added 545 mg of 5-isoquinolinesulfonyl chloride.HCl and 450 mg of triethylamine at a room temperature with stirring. The reaction mixture was stirred for 18 hours, alkalized with a sodium bicarbonate aqueous solution and extracted twice with 100 ml of chloroform.
The extract was dried over magnesium sulfate, and the solvent was evaporated off under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1) to obtain 572 mg of the title compound in colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.0 - 1.33 (3H, m), 1.33 1.54 (lH, m), 1.59 - 1.88 (2H, m), 1.88 - 2.25 (4H, m), 2.33 (3H, s), 2.71 (1H, dd, J = 14.28, 6.85 Hz), 2.91 (1H, dd, J = 14.28, 4.57 Hz), 3.18 (2H, m), 4.34 (2H, s), 6.68 (2H, d, J = 9.14 Hz), 6.90 (2H, d, J = 9.14 Hz), 7.14 (4H, s), 8.13 (1H, t, J = 7.42 Hz), 8.18 (1H, t, J = 7.42 Hz), 9.19 (1H, d, J = 7.42 Hz), 8.27 (2H, d, J = 7.42 Hz), 8.40 (1H, dd, J = 7.99, 1.0 Hz), 8.44 (1H, d, J = 6.85 Hz), 8.52 (1H, d, J = 6.28 Hz), 8.68 (1H, d, J = 6.28 Hz), 8.81 (1H, d, J = 6.28 Hz), 9.34 (1H, s), 9.41 (lH, s).
Example 85. N-1-(p-Hydroxybenzyl)-2-F4-(p- methvlbenzyloxvBpiperidinoletholE-isoquinoline- sulfonamide 400 mg of the amorphous compound obtained in Example 84 was dissolved in 2.5 ml of methanol and 2.5 ml of tetrahydrofuran, to the solution was added 4 ml of 1 N sodium hydroxide solution, and the mixture was refluxed for 2 hours. After cooling, the reaction mixture was diluted with water, acidified with citric acid and then alkalized with sodium bicarbonate, and extracted twice with 100 ml of chloroform. The extract was dried over magnesium sulfate, and the solvent was evaporated off under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (20:1) to obtain 172 mg of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.2 - 1.75 (4H, m), 1.85 2.15 (2H, m), 2.2 - 2.58 (4H, m), 2.32 (3H, s), 2.58 2.85 (2H, m), 3.15 - 3.4 (2H, m), 4.41 (2H, s), 4.8 (2H, br), 6.42 (2H, d, J = 9.14 Hz), 6.68 (2H, d, J = 9.14 Hz), 7.14 (2H, d, J = 7.42 Hz), 7.19 (2H, d, J = 7.42 Hz), 7.67 (1H, t, J = 7.42 Hz), 8.19 (1H, d, J = 7.42 Hz), 8.33 - 8.50 (2H, m), 8.58 (lH, d, J = 6.28 Hz), 9.33 (1H, s).
Example 86.
The same procedure as described in Example 84 was repeated except that N-[2-(tert-butoxycarbonylamino)-3 -[p-(2-methoxyethoxymethoxy)phenyl]propyl}-4-(3,4- dichlorobenzyloxy)piperidine was used in place of N-(2-(tert- butoxyCarbonylamino)-3-[p-(2-methoxyethoxymethOxy)phe- nylpropyl}-4-(p-methylbenzyloxy)piperidine, to obtain N-{1-[p-5-isoquinolinesulfonyloxy)benzyl]-2-[4-(3,4- dichlorobenzyloxy) piperidino]ethyl)-5-isoquinoline- sulfonamide in a colorless amorphous form.
H-NMR (CDC13 , 6 ppm): 0.95 - 1.17 (1H, m), 1.17 - 1.34 (2H, m), 1.34 - 1.91 (2H, m), 1.91 - 2.30 (4H, m), 2.72 (1H, dd, J = 13.13, 7.42 Hz), 2.89 (lH, dd, J = 13.13, 4.57 Hz), 3.20 (2H, m), 4.33 (2H, s), 6.68 (2H, d, J = 9.14 Hz), 6.92 (2H, d, J = 9.14 Hz), 7.10 (1H, dd, J = 9.14, 1.71 Hz), 7.36 (lH, d, J = 1.71 Hz), 7.38 (lH, d, J = 9.14 Hz), 7.64 (1H, t, J = 7.42 Hz), 7.68 (lH, t, J = 7.42 Hz), 8.20 (1H, d, J = 7.42 Hz), 8.28 (2H, d, J = 7.42 Hz), 8.39 (1H, dd, J = 7.42, 1.0 Hz), 8.43 (1H, d, J = 6.28 Hz), 8.53 (1H, d, J = 6.28 Hz), 8.69 (lH, d, J = 6.28 Hz), 8.80 (1H, d, J = 6.28 Hz), 9.35 (1H, s), 9.42 (lH, s); IR (KBr) cm 1615, 1375, 1130, 860.
Example 87.
The amorphous compound obtained in Example 86 was subjected to alkaline hydrolysis according to the procedure in Example 85, to obtain N-(l-(p- hydroxybenzyl)-2-[4-(3,4-dichlorobenzyloxy)piperi- dino]ethyl)-5-isoquinolinesulfonamide in a colorless amorphous form.
IR (KBr) cm : 1615, 1375, 1130, 860; 1H-NMR (CDC13 , E ppm): 1.2 - 1.8 (4H, m), 1.9 2.2 (2H, m), 2.2 - 2.6 (4H, m), 2.62 (1H, dd, J = 14.28, 6.85 Hz), 2.75 (1H, dd, J = 14.28, 6.28 Hz), 3.29 (2H, m), 4.33 (2H, br), 4.39 (2H, s), 6.38 (2H, d, J = 8.57 Hz), 6.67 (2H, d, J = 8.57.Hz), 7.12 (1H, dd, J = 8.57, 1.71 Hz), 7.38 (lH, d, J = 8.57 Hz), 7.39 (lH, d, J = 1.71 Hz), 7.67 (1H, t, J = 7.42 Hz), 8.20 (lH, d, J = 7.42 Hz), 8.37 (1H, d, J = 6.28 Hz), 8.40 (1H, dd, J = 7.42, 1.0 Hz), 8.58 (1H, d, J = 6.28 Hz), 9.32 (1H, s).
Reference Example 23.
The same procedure as described in Reference Example 21 was repeated, except that N-benzyloxycarbonyl-o-benzyltyrosine methyl ester was used in place of N-tert-butoxycarbonyl-o-(2methoxyethoxymethyl)tyrosine methyl ester, to obtain 2-benzyloxycarbonylamino-3-(p-benzyloxyphenyl)-1 -chloropropane in colorless amorphous form.
1H-NMR (CDC13 , s ppm): 2.7 - 2.95 (2H, m), 3.49 (1H, dd, J = 11.42, 3.43 Hz), 3.63 (1H, dd, J = 11.42, 4.00 Hz), 4.13 (1H, m), 5.00 (lH, m), 5.04 (2H, s), 5.10 (2H, s), 6.92 (2H, d, J = 7.99 Hz), 7.15 (2H, d, J = 7.99 Hz), 7.3 - 7.5 (5H, m).
To the amorphous compound so obtained was added N-phenylpiperazine, and the same procedure as described in Reference Example 21 was repeated to obtain 1-{2-(benzyloxycarbonylamino)-3-(p-Benzyloxyphenyl)propyl}-4-phenylpiperazine in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.34 (2H, d, J = 6.85 Hz), 2.4 - 2.7 (4H, m), 2.75 - 3.0 (2H, m), 3.14 (4H, t, J = 5.14 Hz), 3.99 (1H, m), 4.90 (1H, m), 5.04 (2H, s), 5.10 (2H, s), 6.75 - 7.0 (5H, m), 7.09 (2H, d, J = 8.57 Hz), 7.2 = 7.5 (12H, m) Example 88. N-81-r(p-BenzYloxY)benzvll-2-(4- phenylpiperazinyl)ethyl}-5-isoquinolinesulfonamide 500 mg of the 1-substituted 4-phenylpiperazine obtained in Reference Example 23 was dissolved in 1 ml of acetic acid, and to the solution was added 2 ml of 30% hydrogen bromide in acetic acid, and the mixture was stirred for 10 minutes and poured on ice. After adding saturated sodium thiosulfate aqueous solution, the reaction mixture was alkalized with saturated sodium bicarbonate aqueous solution, and extracted twice with 150 ml of chloroform.The extract was dried over magnesium sulfate and the solvent was evaporated off under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1), to obtain 235 mg of 1-{2-amino-3- (p-benzyloxyphenyl)propyl}-4-phenylpip- erazine. 235 mg of this compound was dissolved in 5 ml of tetrahydrofuran, and to the solution were added 195 mg of 5-isoquinolinesulfonyl chloride.HCl and 178 mg of triethylamine at a room temperature with stirring, and the mixture was stirred for 16 hours. After adding saturated sodium chloride aqueous solution, the reaction mixture was alkalized with sodium bicarbonate aqueous solution, and extracted twice with 150 ml of chloroform.
The extract was dried over magnesium sulfate, and the solvent was evaporated off under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (50:1) to obtain 280 mg of N-{l-[ (4-benzyloxy)benzyl]-2-(4-phenylpiperazi- nyl)ethyl)-5-isoquinolinesulfonamide in a calorless amorphous form.
H-NMR (CDC13 , 6 ppm): 2.1 - 2.5 (6H, m), 2.7 3.0 (6H, m), 3.37 (lH, m), 4.99 (2H, s), 5.5 (lH, br), 6.71 (2H, d, J = 8.57 Hz), 6.81 (3H, t, J = 8.57 Hz), 6.90 (2H, d, J = 8.57 Hz), 7.24 (2H, t, J = 8.57 Hz), 7.3 - 7.5 (5H, m), 7.68 (lH, t, J = 7.42 Hz), 8.17 (1H, d, J = 7.99 Hz), 8.43 (1H, d, J = 6.28 Hz), 8.46 (lH, d, J = 6.85 Hz), 8.67 (1H, d, J = 6.28 Hz), 9.32 (lH, s).
Example 89. N-82- r 4-(3 4-Dichlorobenzoloxv!pip- eridino]-1-1-[p-(5-isoquinolinesulfonyloxy)ben- zyl)ethyl}-N-methyl-5-isoquinolinesulfonamide 1.70 g of the amorphous compound obtained in Example 86 was dissolved in 10 ml of dimethylformamide, and to the solution was added 93 mg of sodium hydride with stirring in an ice bath, and after stirring for 10 minutes the ice bath was removed. The reaction mixture was stirred at a room temperature for 4 hours, and after adding 150 ml of ethyl acetate, washed three times with water and the washing were extracted with 50 ml of ethyl acetate. The extracts were combined and washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate, and the solvent was evaporated off under a reduced pressure.Resulting residue was applied to a silica gel column (silica gel: Fuji Debison Kagaku, BW-820MH), and eluted with 1% methanol in chloroform to obtain 1.30 g of the title compound in a colorless amorphous form.
IR (CRCl3) cm : 2920, 2810, 1618, 1583, 1563, 1450, 983, 902; 1H-NMR (CDC13 , 6 ppm): 1.30 - 1.52 (2H, m), 1.60 - 1.78 (2H, m), 1.95 - 2.12 (2H, m), 2.15 - 3.00 (6H, m), 2.84 (3H, s), 3.31 (1H, m), 4.10 (1H, m), 4.42 (2H, s), 6.58 (2K, brd, J = 8.5 Hz), 6.87 (2H, brd, J = 8.5 Hz), 7.14 (1H, dd, J = 8.3, 1.9 Hz), 7.39 (1H, d, J = 8.3 Hz), 7.41 (1H, d, J = 1.9 Hz), 7.53 - 7.65 (2H, m), 8.11 (1H, d, J = 8.3 Hz), 8.28 - 8.31 (3H, m), 8.32 (lH, dd, J = 7.5, 1.2 Hz), 8.54 (1H, brd, J = 6.1 Hz), 8.57 (1H, d, J = 6.1 Hz), 8.83 (lH, d, J = 6.1 Hz), 9.28 (1H, d, J = 1.0 Hz), 9.41 (lH, d, J = 1.0 Hz).
Example 90. N-2-F4- r ,4 -Dichlorobenzvloxv~) piperidino]-1-(p-hydroxybenzyl)ethyl}-N-methyl-5- isoquinolinesulfonamide 1.04 g of the amorphous compound obtained in Example 89 was dissolved in 10 ml of dimethylsulfoxide, to the solution was added a solution of 152 mg of sodium hydroxide in 2 ml of water, and the mixture was stirred at 800C for 2 hours. After adding 150 ml of ethyl acetate, the reaction mixture was twice washed with 100 ml of water, and the washings were extracted with 50 ml of ethyl acetate. The ethyl acetate layers were combined, washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.Resulting residue was applied to a silica-gel column and eluted with 1% methanol in chloroform to obtain 650 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.50 - 1.67 (2H, m), 1.75 - 1.94 (2H, m), 2.15 - 2.30 (2H, m), 2.40 - 2.96 (6H, m), 3.00 (3H, s), 3.39 (1H, m), 3.96 (1H, m), 4.47 (2H, s), 6.26 (2H, brd, J = 8.5 Hz), 6.61 (2H, brd, J = 8.5 Hz), 6.90 - 7.10 (lH, br), 7.16 (lH, dd, J = 8.3, 1.9 Hz), 7.41 (1H, d, J = 8.3 Hz), 7.44 (lH, d, J = 1.9 Hz), 7.60 (1H, dd, J = 8.0, 7.5 Hz), 8.09 (lH, brd, J = 6.1 Hz), 8.14 (1H, brd, J = 8.0 Hz), 8.31 (lH, dd, J = 7.5, 1.2 Hz), 8.47 (lH, d, J = 6.1 Hz), 9.28 (1H, brs).
Example 91. N-;2-F 4- (3 4-Dichlorobenzvloxv ! Pipe- ridinel-1-(p-methoxybenzYl)etholi-N-methYl-5- isoauinolinesulfonamide 350 mg of the amorphous compound obtained in Example 90 was dissolved in 10 ml of dimethylformamide, and to the solution 82.8 mg of methyl iodide was added with stirring in an ice bath. The mixture was stirred for 30 minutes, and after removing the ice bath further stirred for 2 hours at a room temperature. To the reaction mixture was added 100 ml of ethyl acetate, and the mixture was washed three times with 50 ml of water.
The washing were extracted with 50 ml of ethyl acetate, the ethyl acetate layer was washed with water. The ethyl acetate layers were combined, washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate, and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with 1% methanol in chloroform to obtain 297 mg of the title compound in a colorless amorphous form.
IR (CHC13) cm 1 2910, 2835, 1615, 1585, 1322, 1125, 986; 1H-NMR (CDC13 , 6 ppm): 1.38 - 1.60 (2H, m), 1.69 - 1.85 (2H, m), 2.06 - 2.22 (2H, m), 2.36 (1H, dd, J = 13.0, 7.3 Hz), 2.51 - 2.76 (4H,-m), 2.88 (lH, m), 2.93 (3H, s), 3.34 (lH, m), 3.73 (3H, s), 4.13 (1H, m), 4.44 (2H, s), 6.50 (2H, dm, J = 8.8 Hz), 6.83 (2H, dm, J = 8.8 Hz), 7.14 (lH, dd, J = 8.0, 1.9 Hz), 7.40 (lH, d, J = 8.0 Hz), 7.43 (1H, d, J = 1.9 Hz), 7.56 (1H, dd, J = 8.3, 7.3 Hz), 8.08 (1H, brd, J = 8.3 Hz), 8.29 (1H, dd, J = 7.3, 1.2 Hz), 8.55 (1H, d, J = 6.3 Hz), 9.23 (1H, brs) Example 92.
The same procedures as described in Examples 34 and 35 were sequentially repeated except that 1-[2amino-3- (p-hydroxyphenyl ) propyl] -4-phenylpiperidine was used in place of 1-[2-amino-3-(p-hydroxyphenyl)propyl]- 4-phenylpiperazine, to obtain N-(l-[p-(5-isoquinoline- sulfonyloxy)benzyl]-2-(4-phenylpiperidino)ethyl}-Nmethyl-5-isoquinolinesulfonamide in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.15 - 1.75 (4H, m), 1.80 - 2.10 (2H, m), 2.20 - 2.50 (3H, m), 2.60 - 2.80 (2H, m), 2.80 - 3.05 (3H, m), 2.86 (3H, s), 4.13 (1H, d, J = 6.85 Hz), 6.62 (2H, d, J = 7.99 Hz), 6.91 (2K, d, J = 7.99 Hz), 7.12 (2H, dd, J = 6.85, 1.0 Hz), 7.16 - 7.40 (3H, m), 7.58 (lox, t, J = 7.42 Hz), 7.61 (lH, t, J = 7.42 Hz), 8.11 (1H, d, J = 7.42 Hz), 8.26 (3H, dd, J = 6.85, 1.0 Hz), 8.36 (lH, dd, J = 7.42, 1.0 Hz), 8.56 (lH, d, J = 6.28 Hz), 8.59 (lH, d, J = 6.28 Hz), 8.83 (lH, d, J = 6.28 Hz), 9.28 (1H, s), 9.41 (lH, d, J = 1.0 Hz).
Example 93.
The amorphous compound obtained in Example 92 was subjected to alkaline hydrolysis according to the procedure in Example 36, to obtain N-[l-(p-hydroxy benzyl)-2-(4-phenylpiperidino)ethyl]-N-methyl-5-iso- quinolinesulfonamide in a colorless amorphous form.
IR (KBr) cam : 1615, 1515, 1325, 1125; 1H-NMR (CDC13 , 6 ppm): 1.55 - 1.95 (5H, m), 2.22 (2H, dt, J = 6.28, 1.7 Hz), 2.35 - 2.60 (3H, m), 2.70 (lH, dd, J = 12.56, 5.71 Hz), 2.8 - 3.25 (3H, m), 3.06 (3H, s), 3.98 (lH, m), 6.23 (2H, d, J = 8.57 Hz), 6.59 (2H, d, J = 8.57 Hz), 7.15 - 7.40 (SH, m), 7.62 (lH, t, J = 7.42 Hz), 8.10 (lH, d, J = 6.85 Hz), 8.16 (lH, d, J = 7.42 Hz), 8.35 (1H, dd, J = 7.42, 1.0 Hz), 8.47 (lH, d, J = 6.28 Hz), 8.29 (lH, s) Example 94.
The same procedures as described in Examples 34 and 35 were sequentially repeated except that 1-[2amino-3- (p-hydroxyphenyl )propyl]-4,4-ethylenedioxy- piperidine was used in place of 1-[2-amino-3-(p-hydroxyphenyl)propyl]-4-phenylpiperazine, to obtain N-{2-(4,4- ethylenedioxypiperidino ) -1- [p- (S-isoquinolinesulfonyl- oxy)benzyl]ethyl}-N-methyl-5-isoquinolinesulfonamide in a colorless amorphous form.
1H-NMR (CDC13 , E ppm): 1.4 - 1.6 (4H, m), 2.22 (1H, dd, J = 12.56, 6.85 Hz), 2.25 - 2;5 (5H, m), 2.6 2.8 (1H, m), 2.8 - 2.95 (lH, m), 2.84 (3H, s), 3.90 (4H, s), 4.11 (1H, q, J = 6.85 Hz), 6.61 (2H, d, J = 8.57 Hz), 6.89 (2H, d, J = 8.57 Hz), 7.61 (lH, t, J = 7.42 Hz), 7.63 (lH, t, J = 7.42 Hz), 8.12 (lH, d, J = 7.42 Hz), 8.26 (2H, d, J = 7.42 Hz), 8.27 (1H, d, J = 7.42 Hz), 8.35 (lH, d, J = 7.42 Hz), 8.57 (lH, d, J = 6.28 Hz), 8.58 (1H, d, J = 6.28 Hz), 8.84 (lH, d, J = 6.28 Hz), 9.29 (1H, s), 9.42 (lH, s).
Example 95. N-{1-[p-(5-Isoquinolinesulfonyl- oxy)benzyl]-2-(4-oxopiperidino)ethyl}-N-methyl-5- isoquinolinesulfonamide 2.57 g of the product of Example 94 was dissolved in 50 ml of 3 N hydrochloric acid, and after ref fluxing for 6 hours and then cooling, the reaction mixture was alkalized with saturated sodium bicarbonate aqueous solution and extracted twice with 200 ml of chloroform.
The extract was dried over magnesium sulfate and evaporated under a reduced pressure to remove the solvent, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (50:1) to obtain 2.22 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 2.1 - 2.25 (4H, m), 2.31 (1H, dd, J = 13.13, 6.85 Hz), 2.4 = 2.65 (6H, m), 2.65 2.85 (1H, m), 2.79 (3H, s), 4.10 (1H, q, J = 6.85 Hz), 6.52 (2H, d, J = 7.71 Hz), 6.78 (2H, d, J = 7.71 Hz), 7.49 (1H, t, J = 7.42 Hz), 7.55 (1H, t, J = 7.42 Hz), 8.05 (1H, d, J = 7.99 Hz), 8.10 - 8.20 (3H, m), 8.20 (1H, d, J = 7.42 Hz), 8.46 (1H, d, J = 6.28 Hz), 8.48 (1H, d, J = 6.28 Hz), 8.76 (lH, d, J = 6.28 Hz), 9.20 (1H, s), 9.34 (1H, s).
Example 96. N-2-r4-(N'-Benzyl-N'-methyl- amino piperidino 1-1- F p- 5-isosuinolinesulf onvloxv ) benzyllethYli-N-methYl-5-isoquinolinesulfonamide 1.0 g of the product of Example 95 was dissolved in 15 ml of methanol, to the solution were added 270 mg of benzylmethylamine and 120 mg of sodium cyanoborohydride at a room temperature with stirring, and the reaction mixture was stirred for 18 hours. After addition of saturated sodium bicarbonate aqueous solution, the reaction mixture was twice extracted with 150 ml of chloroform.The extract was dried over magnesium sulfate and evaporated under a reduced pressure to remove the solvent, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1), to obtain 380 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , S ppm): 1.0 - 1.45 (2H, m), 1.45 1.65 (1H, m), 1.65 - 2.0 (3H, m), 2.13 (3H, s), 2.15 2.45 (3H, m), 2.5 - 2.9 (4H, m), 2.84 (3K, s), 3.49 (3H, s), 4.07 (1H, q, J = 6.85 Hz), 6.61 (2H, d, J = 7.99 Hz), 6.89 (2H, d, J = 7.99 Hz), 7.28 (5H, s), 7.58 (1H, t, J = 7.42 Hz), 7.60 (1H, t, J = 7.42 Hz), 8.11 (1H, d, J = 7.99 Hz), 8.25 (1H, d, J = 6.28 Hz), 8.26 (2H, d, J = 7.42 Hz), 8.34 (1H, dd, J = 7.42, 1.0 Hz), 8.55 (1H, d, J = 6.28 Hz), 8.57 (lH, d, J = 6.28 Hz), 8.83 (1H, d, J = 6.28 Hz), 9.26 (lH, s), 9.41 (lH, s).
Example 97. N-2-r4-(N-Benzyl-N-methylamino) piperidino 1-1- (p-hydroxybenzyl ethyl -N-methyl-S- isoquinolinesulfonamide 380 mg of the amorphous compound obtained in Example 96 was dissolved in 2 ml of tetrahydrofuran and 2 ml of methanol, to the solution was added 4 ml of 1 N sodium hydroxide aqueous solution, and the mixture was refluxed for 3 hours and cooled. The reaction mixture was poured to water, acidified with citric acid and then alkalized with sodium bicarbonate, washed with a small amount of methanol, and extracted twice with 100 ml of chloroform.The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (20:1) to obtain 147 mg of the title compound in a colorless amorphous form.
H-NMR (CDCl3 , 6 ppm): 1.35 - 1.95 (4H, m), 1.95 - 2.25 (2H, m), 2.19 (3H, s), 2.3 - 2.7 (4H, m), 2.75 3.15 (3H, m), 3.01 (3H, s), 3.57 (2K, s), 3.95 (lH, m), 6.25 (2H, d, J = 8.3 Hz), 6.58 (2H, d, J = 8.55 Hz), 7.2 - 7.4 (5H, m), 7.60 (lH, t, J = 7.57 Hz), 8.11 (lH, d, J = 5.86 Hz), 8.12 (lah, d, J = 8.06 Hz), 8.32 (lH, d, J = 7.33 Hz), 8.46 (1H, d, J = 6.35 Hz), 9.28 (1K, 5).
Example 98.
The same procedures as described in Examples 96 and 97 were sequentially repeated except that benzylamine was used in place of benzylmethylamine, to obtain N-{2-[4-(N-benzylamino)piperidino]-1-(p-hydroxyben- zyl)ethyl}-N-methyl-5-isoquinolinesulfonamide in a colorless amorphous form.
1H-NMR (CDC13 , s ppm): 1.2 - 1.6 (2H, m), 1.7 2.0 (2H, m), 2.0 - 2.3 (2H, m), 2.35 - 2.7 (4H, m), 2.7 - 3.5 (3H, m), 3.0 (3H, s), 3.83 (2H, s), 3.95 (lH, m), 6.24 (2H, d, J = 7.99 Hz), 6.56 (2H, d, J = 7.99 Hz), 7.32 (5H, m), 7.59 (lH, t, J = 7.42 Hz), 8.10 (lH, d, J = 6.28 Hz), 8.12 (lH, d, J = 7.42 Hz), 8.30 (lH, d, J = 7.42 Hz), 8.46 (lH, d, J = 6.28Hz), 9.26 (lH, s).
Example 99. N-{2-(4-hydroxypiperidino)-1- [p-(5- isoquinolinesulfonoloxy)benzyl]ethyl}-N-methyl-5- isoquinolinesulfonamide 200 mg of the amorphous compound obtained in Example 95 was dissolved in 5 ml of methanol, to the solution was added in portions 35.2 mg of sodium borohydride at a room temperature with stirring, and the mixture was stirred for 2 hours and evaporated to remove the solvent. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:4), to obtain 116 mg of the title compound as pale yellow oil.
IR (KBr) cm 1 1620, 1500, 1370, 1320, 1130, 860; 1H-NMR (CDC13 , 6 ppm): 1.2 - 1.4 (2H, m),. 1.5 1.8 (4H, m), 1.9 - 2.1 (2H, m), 2.25 (1H, dd, J = 12.6, 7.3 Hz), 2.4 (lH, dd, J = 12.7, 7.1 Hz), 2.7 (lH, dd, J = 13.8, 7.0 Hz), 2.8 - 2.95 (lH, m), 2.55 (lH, brs), 2.83 (3H, s), 3.55 (lH, m), 4.1 (1H, m), 6.6 (2H, d, J = 8.5 Hz), 6.87 (2H, d, J = 8.5 Hz), 7.6 (1H, t, J = 8.3 Hz), 7.63 (lH, t, J = 7.8 Hz), 8.12 (lH, d, J = 8.3 Hz), 8.23 (lH, d, J = 5.1 Hz), 8.26 (lH, d, J = 5.1 Hz), 8.3 (lH, dd, J = 1.2, 3.3 Hz), 8.54 (1H, d, J = 4.1 Hz), 8.57 (1H, d, J = 4.1 Hz), 8.83 (lH, d, J = 6.1 Hz), 9.28 (lH, s), 9.4 (lH, s).
Example 100. N-{ Hz2 - ( 4 {2-(4-Hydroxypiperidino)-1-[p-(5 - isoquinolinesulfonyloxy)benzyl]ethyl}-N-methyl- 5-isoauinolinesulfonamide 150 mg of the oil obtained in Example 99 was dissolved in 3 ml of methanol, to the solution was added 1 ml of 10% sodium hydroxide aqueous solution, and the reaction mixture were ref lured for 2 hours and evaporated to remove the solvent under a reduced pressure, the resulting residue was acidified with citric acid and then alkalized with a sodium bicarbonate aqueous solution, and the mixture was extracted three times with 20 ml of chloroform.The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, and the resulting residue was subjected to a silica gel preparative thin layer chromatographic and separated by chloroform/methanol (20:1), to obtain 87 mg of the title compound in a colorless amorphous form.
IR (RBr) cm 1 1610, 1510, 1320, 1150, 1125; 1H-NMR (CDC13 , 6 ppm): 1.4 - 1.7 (2H, m), 1.8 2.0 (2H, m), 2.1 - 2.4 (4H, m), 2.3 - 2.6 (1H, m), 2.7 (1H, dd, J = 12.7, 4.8 Hz), 2.8 (1H, dd, J = 14.6, 4.7 Hz), 2.95 (1H, dd, J = 12, 2.5 Hz), 2.99 (3H, s), 3.7 (1H, m), 3.9 (1H, m), 6.15 (2H, d, J = 8.3 Hz), 6.5 (2H, d, J = 8.3 Hz), 7.6 (1H, t, J = 7.6 Hz), 8.0 (1H, d, J = 6.1 Hz), 8.15 (1H, d, J = 8.3 Hz), 8.33 (lH, dd, J = 7.3, 1.0 Hz), 8.4 (1H, d, J = 6.1 Hz), 9.24 .(lH, s).
Example 101. N-r 1- (p-Hydroxybenzyl )-2- (4-hydroxy- PiPeridino)ethYll-N-methol-5-isoauinolinesulfon- amide 100 mg of the amorphous compound obtained by Example 100 was dissolved in 5 ml of a mixture of ethyl acetate/methanol (1:1), to the solution was added an excess amount of diazomethane in ether, and the reaction mixture was allowed to stand overnight at a room temperature. The solvent in the reaction mixture was evaporated off under a reduced pressure, and resulting residue was subjected to a silica gel preparative thin layer chromatography and separated by chloroform/methanol (20:1) to obtain 80.4 mg of the title compound in colorless amorphous form.
IR (KBr) cm : 1510, 1320, 1240, 1150, 1120, 1030; 1H-NMR (CDC13 , 6 ppm): 1.3 - 1.5 (2H, m), 1.7 2.0 (4H, m), 2.0 - 2.3 (2H, m), 2.35 (lH, dd, J = 13.0, 7.1 Hz), 2.55 (1H, dd, J = 10.0, 6.9 Hz), 2.62 (1H, dd, J = 10.3, 7.3 Hz), 2.89 (lH, dd, J = 14.8, 6.3 Hz), 2.6 - 2.75 (1H, m), 2.93 (3H, s), 3.6 (lH, m), 4.12 (lH, m), 3.73 (3H, s), 6.5 (2H, d, J = 8.7 Hz), 6.84 (lH, d, J = 8.7 Hz), 7.56 (lH, t, J = 8.0 Hz), 8.1 (1H, d, J = 8.3 Hz), 8.2 (1H, d, J = 6.1 Hz), 8.3 (lH, d, J = 7.3 Hz), 8.55 (1H, d, J = 6.1 Hz), 9.24 (lH, s).
Example 102. N-[1-(p-Acetoxybenzyl)-2-(4-acetoxy tiperidino ! ethyl 1 -N-methol-5-isoquinolinesulf on- amide 230 mg of the amorphous compound obtained in Example 100 was dissolved in 2 ml of pyridine, to the solution was added 1 ml of acetic anhydride, and the reaction mixture was allowed to stand overnight at a room temperature. To the mixture was added 20 ml of ice water, and the mixture was stirred for one hour and extracted twice with 20 ml of ethyl acetate. The extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 234.3 mg of the title compound in a colorless amorphous form.
IR (KBr) cm : 1760, 1730, 1360, 1320, 1240, 1215, 1200, 1030; 1H-NMR (CDCl3 , 6 ppm): 1.58 (3H, s), 2.03 (3H, s), 2.30 (3H, s), 2.91 (3H, s), 1.4 - 1.8 (4H, m), 2.2 2.9 (4H, m), 4.2 (1H, m), 4.7 (1H, m), 6.77 (2H, d, J = 6.6 Hz), 6.8 (2H, d, J = 6.6 Hz), 7.5 (lH, t, J = 8.0 Hz), 8.1 (lH, d, J = 8.1 Hz), 8.2 (lH, d, J = 7.4 Hz), 8.29 (lH, d, J = 6.1 Hz), 8.57 (1H, d, J = 6.1 Hz), 9.27 (lH, s).
Example 103.
The same procedure as described in Example 84 was repeated except that N-(2-(tert-butoxycarbonylamino)-3- [p- ( 2-methoxyethoxymethoxy)phenyl )propyl}-4-acetoxypipe- ridine synthesized in a similar manner was used in place of N-{2-(tert-butoxycarbonylamino)-3-[p-(2 -methoxyethoxymethoxy)phenyl]propyl}-4-(p- methylbenzyloxy)piperidine, to obtain N-{ 2- [4- acetoxypiperidino]-l-[p-(5-isoquinolinesulfonylOxy)ben- yl]ethyl)-5-isoquinolinesulfonamide in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 0.8 - 1.05 (lH, m), 1.2 1.55 (3H, m), 1.6 - 2.15 (5H, m), 1.99 (3H, s), 2.15 2.33 (1H, m), 2.73 (1H, dd, J = 13.13, 6.85 Hz), 2.89 (lH, dd, J = 13.13, 4.57 Hz), 3.22 (lah, m), 4.51 (1H, m), 5.43 (1H, br), 6.70 (2H, d, J = 8.57 Hz), 6.91 (lH, d, J = 8.57 Hz), 7.66 (1H, t, J = 7.42 Hz), 7.68 (lH, d, J = 7.42 Hz), 8.20 (lH, d, J = 7.42 Hz), 8.30 (2H, d, J = 7.42 Hz), 7.39 (lH, dd, J = 7.42, 1.0 Hz), 8.42 (lH, d, J = 6.28 Hz), 8.53 (lH, d, J = 6.29 Hz), 8.70 (lH, d, J = 6.28 Hz), 8.81 (1H, d, J = 6.28 Hz), 9.34 (lH, s), 9.43 (lH, s).
Example 104. N-2-T4-Hvdroxy,iperidinol-l-rp-(5- isoquinolinesulfonyloxy)benzyl]ethyl}-5-iso- quinolinesulfonamide 1.5 g of the product obtained in the Example 103 was dissolved in 8 ml of methanol, to the solution was added 8 ml of 1 N sodium hydroxide aqueous solution, and the mixture was stirred for two hours, and after adding water, extracted twice with 100 ml of chloroform. The extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. The Resulting residue was applied to a silica gel column an eluted with chloroform/methanol (30:1), to obtain 800 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 0.6 - 0.9 (lH, m), 1.05 1.3 (lH, m), 1.3 - 1.5 (2H, m), 1.5 - 1.9 (3H, m), 1.9 2.2 (2H, m), 2.2 - 2.4 (lH, m), 2.72 (1H, dd, J = 13.70, 6.85 Hz), 2.89 (1H, dd, J = 1.82 4.57 Hz), 3.19 (1H, m), 3.46 (1H, m), 6.72 (2H, d, J = 8.57 Hz), 6.72 (2H, d, J = 8.57 Hz), 7.66 (1H, t, J = 7.42 Hz), 7.69 (1H, t, J = 7.42 Hz), 8.21 (1H, d, J = 7.99 Hz), 8.29 (2H, d, J = 7.42 Hz), 8.41 (1H, d, J = 7.99 Hz), 8.44 (1H, d, J = 6.28 Hz), 8.53 (1H, d, J = 6.28 Hz), 8.69 (1H, d, J = 6.28 Hz), 8.81 (lH, d, J = 6.28Hz), 9.35 (1H, s), 9.42 (1H, s).
Example 105. N-3 ,4-Dichlorobenzyl-N- r 1- ( D-hvdroxy- benzvl -2- (4-hydroxypiperidino ethvl 1-S-iso- quinolinesulfonamide 400 mg of the amorphous compound obtained in Example 104 was dissolved in 5 ml of dimethylformamide, to the solution were added 130 mg of 3,4-dichlorobenzyl chloride and 28 mg of 60% sodium hydride with stirring under ice cooling, and the mixture was allowed to warm to a room temperature and stirred for 18 hours. After adding saturated sodium chloride aqueous solution, the reaction mixture was extracted twice with 100 ml of chloroform, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1), to obtain 228 mg of N-(3,4-dichlorobenzyl)-N -{2-(4-hydroxypiperidino)-1-[P-(5-isoquinolinesulfonyl oxy)benzyl )ethyl}-5-isoquinolinesulfonamide.
228 mg of the above compound was dissolved in 1.5 ml of methanol, to the solution was added 1 ml of 1 N sodium hydroxide aqueous solution, and the mixture was refluxed for 3 hours and then cooled, and after dilution with water, acidified with citric acid and then alkalized with sodium bicarbonate, and extracted twice with 100 ml of chloroform. The extract was dried over magnesium sulfate and evaporated to remove the solvent, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (20:1) to obtain 162 mg of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.2 - 1.6 (2H, m), 1.6 2.0 (3H, m), 2.0 - 2.2 (2H, m), 2.4 - 2.9 (SH, m), 3.5 (1H, m), 4.21 (1H, q, J = 6.08 Hz), 4.44 (1H, d, J = 16.36 Hz), 4.66 (1H, d, J = 16.11 Hz), 6.51 (2H, d, J = 8.55 Hz), 6.79 (2H, d, J = 8.55 Hz), 7.17 (1H, dd, J = 8.30, 1.96 Hz), 7.25 (1H, d, J = 8.06 Hz), 7.33 (lH, d, J = 1.96 Hz), 7.57 (1H, t, J = 7.57 Hz), 8.12 (1H, d, J = 8.3 Hz), 8.25 (1H, d, J = 6.10 Hz), 8.33 (1H, dd, J = 7.32, 0.98 Hz), 8.50 (1H, d, J = 5.86 Hz), 9.21 (1H, s).
Example 106. N-[1-(p-Hydroxybenzyl)-2-(4-hydroxy- piperidino ! ethyl 1 -N-p-methylbenzol-5-iso- quinolinesufonamide The same procedure as described in Example 105 was repeated except that 4-methylbenzyl chloride was used in place of 3,4-dichlorobenzyl chloride was used to obtain the title compound in colorless amorphous form.
1H-NMR (CDC13 + CD30D, s ppm): 1.2 - 1.55 (2H, m), 1.55 - 2.15 (5H, m), 2.33 (3H, s), 2.33 - 2.85 (5H, m), 3.5 (1H, m), 3,98 (lH, q, J = 6.59 Hz), 4.52 (1H., d, J = 15.63 Hz), 4.78 (lH, d, J = 15.62 Hz), 6.36 (2H, d, J = 8.05 Hz), 6.66 (2H, d, J = 8.06 Hz), 7;08 (2H, d, J = 7.56 Hz), 7.29 (2H, d, J = 7.57 Hz), 7.60 (1H, t, J = 7.57 Hz), 8.12 (lH, d, J=8.06 Hz), 8.28 (1H, d, J = 6.10 Hz), 8.39 (1H, d, J = 7.32 Hz), 8.48 (lH, d, J = 6.35 Hz), 9.20 (1H, s).
Example 107. N-r2-(4-HvdroxypiDeridino)-l-p-(5- isoquinolinesulfonyloxy)benzyl]ethyl}-N-methyl-5- isoauinolinesulfonamide The amorphous compound obtained in Example 103 was treated with methyl iodide according to the procedure in Example 89 and the intermediate product was subjected to alkaline hydrolysis according to the procedure in Example 104, to obtain the same product as in Example 99.
1H-NMR (CDC13 , 6 ppm): 1.20 - 1.42 (2H, m), 1.68 (2H, m), 2.01 (2H, m), 2.22 (1H, dd, J = 7.3, 13.2 Hz), 2.40 (1H, dd, J = 7.3, 13.2 Hz), 2.43 - 2.60 (2H, m), 2,66 (1H, dd, J = 6.8, 13.7 Hz), 2.84 (3H, S), 2.85 (lH, dd, J = 6.4, 13.7 Hz), 3,58 (1H, m), 4.10 (1H, m), 6.61 (2H, d, J = 8.8 Hz), 6.88 (2H, d, J = 8.8 Hz), 7.59 (lH, t, J = 7.8 Hz), 7.63 (lH, t, J = 7.8 Hz), 8.12 (1H, d, J = 8.3 Hz), 8.23 - 8.35 (4H, m),8.55 (1H, d, J = 6.4 Hz), 8.58 (1H, d, J = 6.4 Hz), 8.83 (1H, d, J = 5.9 Hz), 9.29 (1H, s), 9.42 (1H, s) Reference Example 24. 1- (N-Benzyloxycarbonyl- tyrosyl -4-phenylpiperazine 12.3 g of N-benzyloxycarbonyltyrosine and 6.6 g of N-phenylpiperazine were dissolved in 150 ml of methylene chloride, and to the solution was added 8.4 g of DCC, and the mixture was stirred at a room temperature for 5 hours. Precipitated insoluble matter was filtered off and the filtrate was concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (1:1 to 1:2) to obtain 10.5 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 2.63 (lH, m), 2.88 - 3.24 (6H, m), 3.48 (lH, m), 3.68 (2H, m), 4-88 (1H, m), 5.07 (1H, d, J = 12.7 Hz), 5.11 (1H, d, J = 12.7 Hz), 5.34 (1H, br), 5.67 (lH, d, J = 8.8 Hz), 6.71 (2H, d, J = 8.3 Hz), 6.84 (2H, d, J = 8.3 Hz), 6.90 (lH, t, J = 7.3 Hz), 7.04 (2H, d, J = 8.3 Hz), 7.26 (2H, t, J = 7.3 Hz), 7.34 (5H, s).
Example 108. 1-[N,O-Bis(5-isopuinolinesulfonyl - tyrosyl 1 -4-phenylpiperazine 4.59 g of the amorphous compound obtained in Reference Example 24 was dissolved in 50 ml of methanol, to the solution was added 3 g of 5% palladium on carbon, and the mixture was stirred for 17 hours at a room temperature in a hydrogen atmosphere. The resulting insoluble matter was filtered off, and the filtrate was concentrated under a reduced pressure to obtain a residue, which was then suspended in 50 ml of chloroform. To the suspension were sequentially added 5.7 g of 5-isoquinolinesulfonyl chloride.HCl and 10 ml of triethylamine with ice cooling, and then mixture was stirred for 3 hours at a room temperature.After adding 200 ml of water, the mixture was extracted twice with 100 ml of chloroform, the extract was dried over magnesium sulfate and concentrated under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (80:1 to 30:1), to obtain 5.46 g of the title compound in a yellow amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.50 - 2.90 (7H, m), 2.92 - 3.17 (2H, m), 3.24 (lH, m), 4.32 (1H, m), 5.99 (lH, br), 6.65 (2H, d, J = 8.8 Hz), 6.80 (2H, d, J = 7.8 Hz), 6.89 (2H, d, J = 8.8 Hz), 6.94 (1H, t, J = 7.3 Hz), 7.29 (2H, dd, J = 7.3, 8.3 Hz), 7.51 (1H, t, J = 7.8 Hz), 7.59 (1H, dd, J = 7.3, 8.3 Hz), 8.09 - 8.31 (5H, m), 8.50 (lH, d, J = 6.4 Hz), 8.69 (1H, d, J = 5.9 Hz), 8.81 (1H, d, J = 5.9 Hz), 9.28 (1H, s), 9.39 (1H, s).
Example 109. l-FN,O-Bis (S-isopuinolinesulònyl )- N-methyltyrosyl 1-4 -phenylpiperazine 2.27 g of the amorphous compound obtained in Example 108 was dissolved in 30 ml of dimethylformamide, to the solution were sequentially added 160 mg of 60% sodium hydride and 0.3 ml of methyl iodide with ice cooling, and the mixture was stirred for 90 minutes with ice cooling. After adding 80 ml of water, the reaction mixture was extracted with 100 ml of ethyl acetate, and the extract was washed with 80 ml of saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (60:1), to obtain 1.8 g of the title compound in a yellow amorphous form.
IR (KBr) cam : 1668, 1475, 1360, 1130; 1H-NMR (CDC13 , 6 ppm): 2.45 (1H, dd, J = 4.6, 13.1 Hz), 2.63 (lH, m), 2.82 - 3.07 (4H, m), 3.03 (3H, s), 3.13 - 3.29 (2H, m), 3.43 - 3.65 (4H, m), 5.11 (1H, dd, J = 4.6, 10.3 Hz), 6.76 (2H, d, J = 8.6 Hz), 6.85 (2H, d, J = 8.0 Hz), 6.88 (1H, t, J = 8.6 Hz), 7.29 (2H, dd, J = 8.0, 8.6 Hz), 7.49 (lH, dd, J = 8.3, 7.3 Hz), 7.70 (1H, dd, J = 8.3, 7.3 Hz), 8.16 (1H, dd, J = 1.0, 7.3 Hz), 8.21 (2H, d, J = 8.3 Hz), 8.30 (1H, dd, J = 1.0, 7.3 Hz), 8.41 (1H, d, J = 6.4 Hz), 8.51 (1H, d, J = 6.4 Hz), 8.68 (1H, d, J = 6.4 Hz), 8.80 (1H, d, J = 6.4 Hz), 9,36 (lH, s), 9.40 (lH, s).
Example 110. 110. (S-Isouinolinesulfonyl)-N- methyltyrosyl]-4-phenylpiperazine 1.15 g of the amorphous compound obtained in Example 109 was suspended in 20 ml of methanol, to the solution was added 2 ml of 2 N sodium hydroxide aqueous solution, and the mixture was refluxed for 90 minutes.
After adding 100 ml of water, the reaction mixture was extracted twice with 50 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (80:1 to 50:1), to obtain 820 mg of the title compound in a colorless amorphous form.
IR (KBr) cm-1 : 1638, 1590, 1440, 1326 1150; 1H-NMR (CDCl3 , E ppm): 2.56 (1H, dd, J = 5.4, 12.7 Hz), 2.61 (1H, m), 2.90 - 3.22 (3H, m), 3.15 (3H, s), 3.43 (lH, m), 3.51 - 3.71 (4H, m), 5.13 (lH, dd, J = 5.9, 9.8 Hz), 5.53 (lH, br), 6.62 (2H, d, J = 8.8 Hz), 6.84 (2H, d, J = 7.8 Hz), 6.89 (1H, t, J = 7.3 Hz), 6.90 (2H, d, J = 8.3 Hz), 7.26 (2H, t, J = 7.8 Hz), 7.70 (1H, dd, J = 7.3, 8.3 Hz), 8.21 (1H, d, J = 8.3 Hz), 8.32 (1H, dd, J = 1.0, 7.3 Hz), 8.38 (1H, d, J = 5.9 Hz), 8.66 (1H, d, J = 5.9 Hz), 9.33 (1H, br).
Example 111.
The product of Example 108 was subjected to alkaline hydrolysis according to the procedure described in Example 110 to obtain 1-[N-(5-isoquinoline- sulfonyl)tyrosyl]-4-phenylpiperazine in a yellow amorphous form.
IR (KBr) cm : 1630, 1590, 1510, 1440, 1325, 1220, 1150, 1128; H-NMR (CDCl3 - CD3OD, 6 ppm): 2.60 (1H, m), 2.72 - 2.77 (2H, m), 2.88 (4H, m), 3.10 - 3.43 (3H, m), 4.37 (1H, t, J = 7.8 Hz), 6.40 (2H, d, J = 8.3 Hz), 6.72 (2H, d, J =8.3 Hz), 6.83 (2H, d, J = 7.8 Hz), 6.91 (1H, t, J = 7.3 Hz), 7.27 (2H, dd, J = 7.8, 8.3 Hz), 7.63 (1H, dd, J = 7.3, 8.3 Hz), 8.17 (1H, d, J = 8.3 Hz), 8.30 (1H, dd, J = 1.0, 7.3 Hz), 8.38 (lH, d, J = 6.4 Hz), 8.60 (1H, d, J = 6.4 Hz), 9.24 (lH, s).
Reference Example 25.
N-Benzyloxycarbonylhomopiperazine To 230 ml of dimethylformamide were added 25 g of homopiperazine and 5.4 g of sodium bicarbonate and then 25 ml of water followed by dropwise addition of 10 g of benzyloxycarbonyl chloride with stirring under ice cooling, and the mixture was stirred at a room temperature overnight. After evaporating off dimethylformamide under a reduced pressure, the reaction mixture was extracted three times with 100 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (9:1), to obtain 9 g of the title compound as a light yellow liquor liquid.
IR (KBr) cm 1 1695, 1420; H-NMR (CDC13 , E ppm): 1.8 (2H, m), 2.8 - 3.0 (4H, m), 3.4 - 3.65 (4H, m), 5.15 (2H, s), 7.4 (5H, s).
Reference Example 26. 1-rN- (tert-Butoxycarbonyl - N-methyl 1 tyrosyl-4-benzyloxycarbonylhomopiperazine 1.0 g of N-tert-butoxycarbonyl-N-methyltyrosine was dissolved in 70 ml of methylene chloride, and after adding 793 mg of N-benzyloxycarbonylhomopiperazine and adding at a stroke 837 mg of DCC at a room temperature with stirring, the mixture was stirred at a room temperature overnight. The solvent was evaporated off under a reduced pressure, and to the resulting residue was added benzene. Insoluble matter was filtered off, and the filtrate was applied to a silica gel column and eluted with hexane/ethyl acetate (6:4 to 6:5) to obtain 1.06 g of the title compound as a light yellowish oil.
Acetylated derivative of this compound has the following properties.
IR (KBr) cm 1 1760, 1695, 1215, 1200; 1H-NMR (CDC13 , S ppm): 1.3, 1.4 (Total 9H, each s), 1.85 (lH, m), 2.3 (3H, s), 2.82 (3H, brs), 2.7 - 2.9 (2H, m), 3.0 - 3.8 (8H, m), 5.15 (2H, brs), 7.0 (2H, d, J = 8.3 Hz), 7.35 (5H, brs).
Reference Example 27. 1- 3, - (p-Acetoxyphenyl - 2'[N-(tert-butoxyzarbonol)-N-methylaminolProPvlo- 4-benzvloxYcarbonolhomopiPerazine 3.56 g of the product obtained in Reference Example 26 was dissolved in 60 ml of absolute tetrahydrofuran, to the solution was added 20 ml of 1.0 M borane in tetrahydrofuran with stirring under ice cooling, and the mixture was stirred overnight at a room temperature. The solvent was evaporated off under a reduced pressure, and resulting residue was dissolved in 10 ml of pyridine. To the solution was added 5 ml of acetic anhydride, and the mixture was allowed to stand overnight at a room temperature. After an addition of ice, the mixture was stirred for 30 minutes and extracted twice with 60 ml of chloroform.The extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1), to obtain 2.0 g of the title compound in a light yellow amorphous form.
cm-1 IR (KBr) cm : 1760, 1690, 1215, 1200; 1H-NMR (CDC13 , E ppm): 1.25, 1.27 (Total 9H, each s), 1.6 - 1.9 (2H, m), 2.27 (3H, s), 2.4 - 2.8 (llH, m), 3.4 - 3.6 (4H, m), 5.13 (2H, brs), 6.97 (2H, d, J = 8.6 Hz), 7.1 (2H, d, J = 8.6 Hz), 7.25, 7.33 (Total 5K, each s).
ExamPle 112. N- r 1- (D-Acetoxvbenzvl)-2-(4-benzvl- oxYcarbonvlhomociPerazinvl!etholl-N-methY1-5- isocuinolinesulfonamide 1 g of the amorphous compound obtained in Reference Example 27 was dissolved in 28 ml of methylene chloride, to the solution were added 2 ml of 2,6-lutidine, and then 2 ml of tert-butyldimethylsilyltrifluoromethane sulfonate with stirring at a room temperature, and the reaction mixture was stirred for 16 hours. After an addition of ice, the reaction mixture was extracted twice with 70 ml of ethyl acetate, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.To resulting residue were added 20 ml of tetrahydrofuran and 4.28 ml of 1.0 M tetrabutylammonium fluoride in tetrahydrofuran with stirring, and the reaction mixture was stirred at a room temperature for 40 minutes. After adding ice, the reaction mixture was extracted twice with 70 ml of chloroform, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (95:5 to 90:10), to obtain 723 mg of 1-[3'-( p-acetoxyphenyl)-2'-( N-methylamino)propyl]-4- benzyloxycarbonylhomopiperazine.
1H-NMR (CDC13 , 6 ppm): 1.8 (2H, m), 2.3 (3H, s), 2.48 (3H, d, J = 2.0 Hz), 2.35 - 3.8 (9H, m), 3.4 - 3.6 (4H, m), 5.1 (2H, s), 7.0 (2H, d, J = 8.5 Hz), 7.2 (2H, brd, J = 8.5 Hz), 7.35 (5H, s).
723 mg of the abovecompound was dissolved in 25 ml of dimethylformamide, and to the mixture was added 401 mg of triethylamine and then 564 mg of 5-isoquinolinesulfonyl chloride.HCl with stirring under ice cooling, and the mixture was stirred overnight at a room temperature. After adding water, the reaction mixture was extracted twice with 70 ml of ethyl acetate, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 796 mg of the title compound in a light yellow amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.7 (2H, m), 2.3 (3H, s), 2.7 - 2.8 (8H, m), 2.90, 2.91 (Total 3H, each s), 3.3 3.55 (4H, m), 4.1 (lH, m), 5.1 (2H, s), 6.7 (2H, d, J = 8.3 Hz), 6.9 (2H, d, J = 8.3 Hz), 7.34, 7.36 (Total 5H, each s), 7.53, 7.55 (Total lH, each t, J = 7.6 Hz), 8.1 (lH, d, J = 6.1 Hz), 8.18 (2H, d, J = 6.5 Hz), 8.55 (1H, d, J = 6.1 Hz), 9.25 (lH, s).
Example 113. N- r 2- (4-Benzyloxycarbonylhomo- piperazinyl ! -1- (P-hodroxYbenzYl)etholl-N-methol-5- isoauinolinesulfonamide 400 mg of the amorphous compound obtained in Example 112 was dissolved in 10 ml of methanol, to the solution was added 2 ml of 10% sodium hydroxide and the mixture was stirred for 10 minutes. The reaction mixture was acidified with citric acid aqueous solution and then alkalized with saturated sodium bicarbonate aqueous solution, and extracted twice with 50 ml of chloroform. The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:2) to obtain 339 mg of the title compound in a colorless amorphous form.
cm-1 IR (KBr) cm : 1700, 1330, 1210, 1150, 1120; 1H-NMR (CDC13 , 6 ppm): 1.8 (2H, m), 1.37, 1.38 (Total lH, each dd, J = 10.0, 13.8 Hz), 1.55 (1H, dd, J = 13.8, 9.8 Hz), 2.75 (4H, m), 2.7 - 3.0 (2H, m), 3.0 (3H, s), 3.5 (4H, m), 3.8 (lH, m), 5.13 (2H, s), 6.17 (2H, d, J = 8.0 Hz), 6.50, 6.51 (Total 2H, each d, J = 8.0 Hz), 7.49, 7.50 (total lH, each t, J = 7.7 Hz), 8.03 (lH, d, J = 6.1 Hz) 8.13 (lH, d, J = 7.8 Hz), 8.23 (1H, d, J = 7.1 Hz), 6.43 (lH, d, J = 6.1 Hz), 9.24 (lH, s), 7.35 (5H, s).
Example 114.
220 mg of the amorphous compound obtained in Example 113 was dissolved in 2 ml of acetic acid, to the solution was added 6 ml of 25% hydrogen bromide in acetic acid, and the mixture was stirred at a room temperature for 20 minutes. 40 ml of dry ether was added to the reaction mixture to form a white precipitate, which was then alkalized with saturated sodium bicarbonate aqueous solution and extracted twice with 20 ml of chloroform/isopropanol (5:1). The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol ( 20:80 two 30:70) to obtain 67 mg of N-[l-(p-hydroxy) benzyl-2-homopiperazinylethyl] -N-methyl-S-isoquinoline- sulfonamide as a light yellow oil.
H-NMR (CDCl3 , 6 ppm): 1.75 (2H, m), 2.3 - 3.0 (12H, m), 2.93 (3H, s), 3.96 (lH, m), 6.3 (2H, d, J = 8.3 Hz), 6.6 (2H, d, J = 8.3 Hz), 7.6 (lH, t, J = 8.1Hz), 8.1 (1H, d, J = 5.3 Hz), 8.12 (lH, d, J = 8.3 Hz), 8.2 (1H, d, J = 7.4 Hz), 8.45 (lH, d, J = 6.1 Hz), 9.26 (1K, s).
Reference Example 28. 1-Benzyloxycarbonyl-4- (N-tert-butoxycarbonyltyrosyl homopiperazine 15.29 g of N-tert-butoxycarbonyltyrosine and 12.73 g of N-benzyloxycarbonylhomopiperazine were dissolved in 280 ml of tetrahydrofuran, to the solution were added 8.09 g of l-hydroxybenzotriazole hydrate and 11.77 g of DCC at a room temperature with stirring, and the reaction mixture was stirred for 16 hours. The reaction mixture was evaporated to remove the solvent under a reduced pressure, to the residue was added benzene, and insoluble matter was filtered by suction and washed with benzene. The benzene layers were combined and evaporated off under a reduced pressure.
The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1), to obtain 26.42 g of the title compound in colorless amorphous form.
1H-NMR-(CDCl3 , 6 ppm): 1.41 (9H, s), 1.5 - 2.0 (2H, m), 2.75 - 3.05 (2H, m), 3.05 - 3.7 (8H, m), 4.67 (lH, m), 5.10, 5.12 (Total 2H, each s), 5.25 (1H, m), 6.0 (1H, br), 6.68, 6.72 (Total 2H, each d, J = 8.57 Hz), 7.02, 7.03 (Total 2H, each d, J = 8.57 Hz), 7.32, 7.34 (Total 5H, each s).
Example 115. N-82- r (4-Benzoloxycarbonol!homo- piperazinyl 1-1- Fp- ( 5-isocruinolinesulfonyloxy) benzvllethvl)-5-isosuinolinesulfonamide 3.0 g of the amorphous compound obtained in Reference Example 28 was dissolved in 20 ml of tetrahydrofuran, to the solution was added 24 ml of 1 M borane in tetrahydrofuran, and the mixture was stirred under a nitrogen atmosphere at a room temperature for 15 hours. After the reaction was completed, the solvent was evaporated off under a reduced pressure and to the resulting residue was added 3 g of potassium bicarbonate. The mixture was stirred for 30 minutes at a room temperature and extracted twice with 200 ml of chloroform.The extract was dried over magnesium sulfate and concentrated under a reduced pressure, to obtain 1-benzyloxycarbonyl-4- [ 2- (tert-butoxycarbonyl -amino)-3-(p-hydroxyphenyl)propyl]homopiperazine.
This compound was dissolved in 6 ml of ethyl acetate, to the solution was added 30 ml of 4 N hydrogen chloride in ethyl acetate, and the mixture was stirred at a room temperature for 30 minutes. The reaction mixture was evaporated under a reduced pressure and the resulting residue was alkalized with a saturated sodium bicarbonate aqueous solution and extracted twice with 200 ml of chloroform. The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, to obtain 2.43 g of 1-[2-amino-3-(p-hydroxyphenyl)propyl]-4-benzylOxy carbonylhomopiperazine.
This intermediate was dissolved in 65 ml of tetrahydrofuran, to the solution were added 4.03 g of 5-isoquinolinesulfonyl chloride.HCl and 8.9 ml of triethylamine at a room temperature with stirring, and the mixture was stirred for 16 hours. After an addition of a saturated sodium bicarbonate solution, the mixture was extracted twice with 300 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.
Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (20:1), to obtain 2.26 g of the title compound in a colorless amorphous form.
H-NMR (CDC13 , b ppm): 1.43 (2H, m), 2.2 (6H, m), 2.73 (2H, m),-2.9 - 3.4 (5H, m), 5.07 (2H, s), 5.34 (lH, br), 6.62 (2H, d, J = 8.57 Hz), 6.84, 6.86 (Total 2H, each d, J = 8.57 Hz), 7.32, 7.33 (Total 5H, each s), 7.63 (1H, t, J = 8.28 Hz), 7.67 (1H, m), 8.16 (lH, t, J = 8.28 Hz), 7.67 (lH, m), 8.16 (lH, t, J = 7.42 Hz), 8.22 - 8.45 (4H, m), 8.53 (lH, d, J = 6.28 Hz), 8.66 (1H, dd, J = 6.57, 1.0 Hz), 8.82 (1H, d, J = 6.28 Hz), 9.31, 9.34 (Total lH, each s), 9.42 (lH, s).
Example 116.
1.0 g of the product obtained in Example 115 was dissolved in 5 ml of methanol and 5 ml of tetrahydrofuran, to the solution was added 10 ml of 1 N sodium hydroxide, and the mixture was refluxed for 2 hours and then cooled. The reaction mixture was acidified with citric acid and then alkalized with sodium bicarbonate, the produced insoluble matter, was then dissolved in methanol. The solution was extracted twice with 100 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (20:1), to obtain 458 mg of N-(2-(4-benzyloxycarbonyl -homopiperazinyl)-1-(p-hydroxybenzyl)ethyl}-5-isoquinolinesulfonamide in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.72 (2H, brs), 2.3 - 2.9 (8H, m), 3.1 - 3.7 (5H, m), 5.12 (2H, s), 6.27 (2H, d, J = 7.32 He),'6.57 (2H, d, J = 7.32 Hz), 7.6 (1H, br), 7.33 (6H, s), 7.63 (1H, t, J = 7.57 Hz), 8.17 (1H, d, J = 8.3 Hz), 8.33 (2H, d, J = 7.08 Hz), 8.52 (1H, brs), 9.28 (1H, brs).
ExamPle 117. N-(1-Fp- (S-Isoauinolinesulfonyloxy)- benzv11-2-homopiperazinslethYlE-5-isoauinoline- sulfonamide To 1.0 g of the product obtained in Example 115, was added 6 ml of 30% hydrogen bromide in acetic acid at a room temperature with stirring, and the mixture was further stirred for 24 hours. After an addtion of 100 ml of ether the reaction mixture was stirred for 30 minutes to form a salt, which was then colected by filtration, washed with ether and dried in a desiccator.
The salt was dissolved in water, and the solution was alkalized with sodium bicarbonate and extracted twice with 100 ml of chloroform. The extract was dried with magnesium sulfate and evaporated to remove the solvent under a reduced pressure, to obtain 830 mg of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.37 (2H, m), 2.1 - 2.9 (12H, m), 3.22 (lH, m), 6.62 (2H, d, J = 8.79 Hz), 6.87 (2H, d, J = 8.54 Hz), 7.64 (1H, t, J = 7.82 Hz), 7.66 (1H, t, J = 7.82 Hz), 8.18 (1H, d, J = 8.31 Hz), 8.23 8.36 (3H, m), 8.40 (1H, d, J = 6.35 Hz), 8.53 (lH, d, J = 6.1 Hz), 8.67 (1H, d, J = 6.11 Hz), 8.81 (1H, d, J = 6.35 Hz), 9.33 (1H, s), 9.42 (1H, s).
Example 118. N-{1-[p-(5-Isoquinolinesulfonyloxy benzvll-2- r 4-(3-phenolproPionvl ! homoPiserazinvll ethvlE-5-isoquinolinesulfonamide 420 mg of the amorphous compound obtained in Example 117 was dissolved in 6 ml of methylene chloride, to the solution were added 125 mg of 3-phenylpropionyl chloride and 100 mg of triethylamine at a room temperature with stirring, and the mixture was stirred for 17 hours. The reaction mixture was alkalized with a saturated sodium bicarbonate aqueous solution and extracted twice with 50 ml of chloroform. The extract was dried over magnesium sulfate and evaporated to remove the solvent. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1) to obtain 400 mg of the title compound in colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.46 (2H, m), 1.9 - 2.4 (6H, m), 2.4 - 2.6 (2H, m), 2.6 - 2.82 (2H, m), 2.82 2.98 (2H, m), 2.98 - 3.12 (lH, m), 3.12 - 3.33 (.3H, m), 3.4 (lH, t, J = 6.28 Hz), 6.61, 6.63 (Total 2H, each d, J = 8.57 Hz), 6.82, 6.85 (Total 2H, each d, J = 8.57 Hz), 7.1 - 7.35 (5H, m), 7.64 (1H, t, J = 8.28 Hz), 7.66 (1H, t, J = 8.28 Hz), 8.1 - 8.45 (SH, m), 8.52 (1H, d, J = 6.28 Hz), 8.67 (lH, dd, J = 6.28, 1.0 Hz), 8.82 (lH, d, J = 6.28 Hz), 9.33, 9.34 (Total lH, each s), 9.42 (lH, s).
Example 119. N-1-(t-Hydroxybenzyl)-2-F4-(3- phenylpropionyl)homopiperazinyl]ethyl}-5- isoouinolinesulfonylamide 400 mg of the amorphous compound obtained in Example 118 was dissolved in 2 ml of methanol and 2 ml of tetrahydrofuran, to the solution was added 4 ml of 1 N sodium hydroxide, and the mixture was refluxed for 3 hours and then cooled. The reaction mixture was acidified with citric acid and then alkalized with sodium bicarbonate to form an insoluble matter, which was then dissolved in a small amount of methanol and extracted twice with 50 ml of chloroform. The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1), to obtain 230 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , E ppm): 1.64 (2H, m), 2.3 - 2.85 (lOH, m), 2.96 (2H, t, J = 8.3 Hz), 3.15 - 3.6 (5H, m), 6.31, 6.35 (Total 2H, each d, J = 8.30 Hz), 6.57, 6.61 (Total 2H, each d, J = 8.30 Hz), 7.1 - 7.4 (5H, m), 7.65 (1K, t, J = 8.06 Hz), 8.19 (1H, d, J = 8.23 Hz), 8.25 8.4 (2H, m), 8.55 (lH, d, J = 6.28 Hz), 9.32 (lH, s).
Reference Example 29. l-Benzyloxycarbonyl-4- (N-tert-butoxycarbonyl-o-methyl tyrosylhomo- piperazine 5.0 g of the amorphous compound obtained in Reference Example 28 was dissolved in 25 ml of tetrahydrofuran and 25 ml of dimethylformamide, to the solution was added 0.41 g of 60% sodium hydride with stirring under ice cooling, and then the mixture was allowed to warm to a room temperature and stirred for 30 minutes. After adding 1.43 g of methyl iodide, the reaction mixture was stirred for 16 hours, and after an addition of a saturated sodium chloride aqueous solution, extracted twice with 300 ml of chloroform.
The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1), to obtain 3.76 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.41 (9H, s), 1.65 - 2.0 (2H, m), 2.8 - 3.05 (2H, m), 3.05 - 3.65 (8H, m), 3.77 (3H, s), 5.68 (lH, m), 5.10 (2H, s), 5.23 (1H, m), 6.79 (2H, d, J = 8.3 Hz), 7.11 (2H, d, J = 8.54 Hz), 7.33 (5H, s).
Reference Example 30.
1- (N-tert-Butoxycarbonyl-o-methyl tyrosyl-4-phenyl -acetylhomopiperazine 1.02 g of the amorphous compound obtained in Reference Example 29 was dissolved in 25 ml of methanol, to the solution was added 250 mg of 5% palladium on carbon with ice cooling, and after warming the mixture to a room temperature, the catalytic reduction was carried out for 6 hours. The catalyst was filtered off and washed with methanol, and the methanol solution was evaporated to obtain 800 mg of (N-tert-butoxycarbonyl -o-methyl)tyrosylhomopiperazine.
400 mg of the compound was dissolved in 6 ml of methylene chloride, to the solutionwere added 195 mg of phenylacetyl chloride and 190 mg of triethylamine, and the mixture was stirred at a room temperature for 24 hours. The reaction mixture was alkalized with a saturated sodium bicarbonate aqueous solution and extracted twice with 100 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.
Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (50:1), to obtain 433 mg of the title compound in a colorless amorphous form.
H-NMR (CDC13 , 6 ppm): 1.41 (9H, s), 1.6 - 2.0 (2H, m), 2.7 - 3.75 (12H, m), 3.77, 3.78 (Total 3H, each s), 4.65 (1H, m), 5.13, 5.24 (Total 1H, each d, J = 9.14 Hz), 6.78, 6.79 (Total 2H, each d, J = 9.71 Hz), 7.08, 7.11 (Total 2H, each d, J = 9.71 Hz), 7.28 (5H, m).
Example 120. l-rN-(5-IsoQuinolinesulfonvl)- o-methyl 1 tyrosyl-4-phenylacetylhomopiperazine 433 mg of the amorphous compound obtained in Reference Example 30 was dissolved in 1 ml of ethyl acetate, to the solution was added 4 ml of 4 N hydrogen chloride in ethyl acetate, and after stirring for 30 minutes at a room temperature, the solvent was evaporated off under a reduced pressure. To resulting residue was added a saturated sodium bicarbonate aqueous solution, and the solution was twice extracted with 50 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure.To resulting residue were added 6 ml of tetrahydrofuran, as well as 275 mg of 5-isoquinoline -sulfonyl chloride.KC1 and 1.2 ml of triethylamine at a room temperature with stirring, and the mixture was further stirred for 18 hours. The reaction mixture was alkalized with a saturated sodium bicarbonate aqueous solution and extracted twice with 50 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1), to obtain 439 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.5 - 1.9 (2H, m), 2.4 2.9 (3H, m), 2.9 - 4.0 (9H, m), 3.67, 3.68, 3.70 (Total 3H, each s), 4.18 (lH, m), 6.18 (lH, m), 6.25 - 6.5 (2H, m), 6.7 (2H, m), 7.28 (5H, m), 7.54, 7.56 (Total 1H, each t, J = 7.81 Hz), 8.09 (lH, t, J = 7.81 Hz), 8.15 8.3 (2H, m), 8.63 (1H, m), 9.22, 9.26 (Total 1H, each s).
Example 121. l-FN,O-Dimethyl-N- (5-isocuinoline- sulfonyl 1 tyrosyl-4-phenylacetylhomopiperazine 439 mg of the amorphous compound obtained in Example 120 was dissolved in 2.5 ml of tetrahydrofuran and 2.5 ml of dimethylformamide, to the solution was added 30 mg of 60% sodium hydride with ice cooling, and then the mixture was warmed to a room temperature and stirred for 30 minutes. After an addition of 110 mg of methyl iodide, the reaction mixture was further stirred for 16 hours. After an addition of a saturated sodium chloride aqueous solution, the reaction mixture was extracted twice with 50 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.
Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1), to obtain 348 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.5 - 2.0 (2H, m), 2.2 4.0 (12H, m), 3.03, 3.07, 3.08, 3.19 (Total 3H, each s), 3.71, 3.73, 3.75 (Total 3H, each s), 4.9 (1H, m), 6.5 - 6.78 (2H, m), 6.78 - 7.0 (2H, m), 7.26 (5H, m), 7.68 (1H, m), 8.1 - 8.33 (2H, m), 8.42 (1H, m), 8.66 (lH, m), 9.32 (1H, m).
Example 122. 1-Benzyloxycarbonyl-4- FN 0-bis ( 5-isoauinolinesulfonsl ) tyrosyl JhomopiPerazine 6.44 g of the amorphous compound obtained in Reference Example 28 was dissolved in 6 ml of ethyl acetate, to the solution was added 60 ml of 4 N hydrogen chloride in ethyl acetate at a room temperature with stirring, and the mixture was stirred for 3 hours. The reaction mixture was concentrated under a reduced pressure and after an addition of benzene, again.
concentrated under a reduced pressure, to obtain 1-benzyloxycarbonyl- 4 -tyrosylhomopiperåzine/hydrochlo -ride in an amorphous form.
To this intermediate were added 130 ml of tetrahydrofuran as well as 7.88 g of 5-isoquinolinesulfonyl chloride.HCl and 18 ml of triethylamine, and the mixture was stirred for 18 hours at a room temperature. The reaction mixture was alkalized with a saturated sodium bicarbonate aqueous solution and extracted twice with 700 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.
Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (30:1) to obtain 5.50 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , s ppm): 1.65 (2H, m), 2.4 - 3.8 (10H, m), 4.17 (lH, m), 5.1 (2H, m), 6.02 (lH, d, J = 9.52 Hz), 6.47, 6.51 (Total 2H, each d, J = 8.55 Hz), 6.75 (2H, d, J = 8.55 Hz), 7.29, 7.33 (Total 5H, each s), 7.58, 7.60 (Total 1H, each t, J = 8.06 Hz), 8.1 8.3 (5H, m), 8.52 (lH, d, J = 6.11 Hz), 8.64 (lH, d, J = 6.10 Hz), 8.84 (lH, d, J = 5.37 Hz), 9.29 (1H, s), 9.41 (lH, s).
Example 123. 1-Benzvloxycarbonyl-4- FN- (S-iso- puinolinesulfonyl tyrosyl 1 homopiperazine 5.50 g of the amorphous compound obtained in Example 122 was dissolved in 30 ml of methanol and 30 ml of tetrahydrofuran, to the solution was added 60 ml of 1 N sodium hydroxide, and the mixture was refluxed for 2 hours and then cooled. The reaction mixture was acidified with citric acid and then alkalized with sodium bicarbonate, resulting insoluble matter was dissolved with a small amount of methanol, and the solution was extracted twice with 400 ml of chloroform.
The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (20:1) to obtain 3.1 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 + CD30D, 6 ppm): 1.82 (2H, m), 2.48 (lH, m), 2.68 (lH, dt, J = 6.85, 5.71 Hz), 3.1 - 3.8 (8H, m), 4.16 (1H, m), 5.12, 5.13 (Total 2H, each s), 6.14, 6.17 (Total 2H, each d, J = 8.55 Hz), 6.52, 6.53 (Total 2H, each d, J = 8.55 Hz), 7.33, 7.35 (Total 5H, each s), 7.61 (1H, m), 8.16 (lH, d, J = 8.06 Hz), 8.2 8.45 (2H, m), 8.53 (lH, d, J = 6.11 Hz), 9.21 (1H, s).
Reference Example 31.
N-Benzyloxycarbonyltyrosine and N-tert-butoxycarbonylhomopiperazine were treated according to the procedure in Reference Example 28 to obtain l-(N-benzyloxycarbonyl)tyrosyl-4-tert butoxycarbonylhomopiperazine in a colorless amorphous form.
H-NMR (CDC13 , 6 ppm): 1.42, 1.44 (Total 9H, each s), 1.6-2.0 (2H, m), 2.7 - 3.8 (10H, m), 4.75 (lH, m), 5.04 (lH, d, J = 11.42 Hz), 5.13 (1H, d, J = 11.42 Hz), 5.5 (lH, m), 6.72 (2H, m), 7.02 (2H, m), 7.34 (5H, s).
Reference Example 32. 1- (O-Acetyl-N-benzyloxy- carbonsl)torosvl-4-tert-butoxycarbonolhomo- tiperazine 690 mg of the amorphous compound obtained in Reference Example 31 was dissolved in 7 ml of pyridine, and to the solution was added 3.5 ml of acetic anhydride at a room temperature with stirring, and the mixture was further stirred for 18 hours. After pouring the reaction mixture to saturated sodium hydroxide aqueous solution to alkalize, the mixture was extracted twice with 100 ml of chloroform. The extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate, and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 670 mg of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 1.41, 1.43 (Total 9H, each s), 1.5 - 2.0 (2H, m), 2.28 (3H, s), 2.8 - 3.7 (lOH, m), 4.7 (lH, m), 5.05 (1H, d, J = 11.4 Hz), 5.13 (lH, d, J = 11.4 Hz), 5.52 (1H, m), 6.99 (2H, d, J = 7.42 Hz), 7.21 (2H, d, J = 7.42 Hz), 7.34 (5H, s).
Reference Example 33. 1- (O-Acetyl-N-benzyloxy- carbonolltyrossl-4-(3-PhenYlPronYl) homopiperazine 670 mg of the amorphous compound obtained in reference Example 32 was dissolved in 2 ml of ethyl acetate, and to the solution was added 7 ml of 3 N hydrogen chloride in ethyl acetate at a room temperature with stirring, and the mixture was further stirred for 30 minutes, alkalized with sodium bicarbonate aqueous solution, saturated with sodium chloride, and extracted twice with 100 ml of ethyl acetate. The extract was dried over magnesium sulfate and evaporated under a reduced pressure, to obtain 460 mg of 1- (O-acetyl-N-benzyloxycarbonyl ) tyrosylhomopiperazine.
This compound was dissolved in 6 ml of dimetylformamide, and to the solution were added 150 mg of potassium carbonate, 160 mg of sodium iodide and 210 mg of l-bromo-3-phenylpropane at a room temperature with stirring, and the mixture was stirred for 20 hours.
After an addition of a saturated sodium chloride aqueous solution, the reaction mixture was extracted twice with 100 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced prssure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 430 mg of the title compound in a colorless amorphous form.
H-NMR (CDC13 , 6 ppm): 1.5 - 2.0 (6H, m), 2.26 (3H, s), 2.3 - 2.7 (6H, m), 2.9 - 3.8 (6H, m), 4.84 (1H, m), 5.03 (1H, d, J = 11.99 Hz), 5.12 (1H, d, J = 11.99 Hz), 5.6 (1H, m), 6.97 (2H, dd, J = 8.57, 1.0 Hz), 7.1 - 7.3 (7H, m), 7.33 (5H, s).
Reference Example 34. 1-(3-Phenylpropyl )-4- tyrosylhomopipera zine 430 mg of the amorphous compound obtained in Reference Example 33 was dissolved in 5 ml of methanol, to the solution was added 120 mg of potassium carbonate at a room temperature with stirring, and the reaction mixture was stirred for 70 hours. After an addition of a saturated sodium chloride aqueous solution, the reaction mixture was acidified with citric acid and extracted twice with 100 ml of chloroform. The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 395 mg of l-(N-benzyloxycarbonyl)tyrosyl-4-(3-phenylpropyl)homopi perazine.
This compound was dissolved in 15 ml of methanol, and to the solution were added 0.05 ml of concentrated hydrochloric acid and 150 mg of 5% palladium on carbon with ice cooling. After warming the reaction mixture to a room temperature, the catalytic reduction was carried out in a hydrogen atmosphere for 8 hours. The palladium on carbon catalyst was filtered suction, and washed with methanol. The filtrates were combined and evaporated to remove the solvent under a reduced pressure, and to resulting residue was added saturated sodium chloride aqueous solution. The mixture was alkalized with a sodium bicarbonate aqueous solution, precipitated insoluble matter was dissolved by adding a small amount of methanol, and the mixture was extracted twice with 80 ml of chloroform.The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (20:1), to obtain 180 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.75 (4H, m), 2.3 .- 2.8 (1OH, m), 2.92 (1H, m), 3.1 - 3.8 (4H, m), 3.86 (1H, q, J = 6.28 Hz), 6.65, 6.66 (Total 2H, each d, J = 8.57 Hz), 6.99, 7.00 (Total 2H, each d, J = 8.57 Hz), 7.1 - 7.35 (5H, m).
Example 124. 1-fN-(5-Isoquinolinesulfonsl)torosoll -4- ( 3-phenylpropyl )homopiperazine 180 mg of the amorphous compound obtained in Reference Example 34 was dissolved in 4 ml of tetrahydrofuran, and to the solution were added 137 mg of 5-isoquinolinesulfonyl chloride.HCl and 0.2 ml of triethylamine at a room temperature with stirring, and the mixture was stirred for 15 hours. After an addition of a saturated sodium chloride aqueous solution, the reaction mixture was alkalized with sodium bicarbonate, and the precipitated insoluble matter was made oily by adding a small amount of methanol, and the mixture was extracted twice with 50 ml of chloroform. The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.Resulting residue was applied to a silica gel column and a preparative thin layer chromatographic plate and eluted with chloroform/methanol (10:1), to obtain 130 mg of the title compound in colorless amorphous form.
1H-NMR (CDC13 + CD30D, 6 ppm): 1.76 (4H, m), 2.3 2.8 (lOH, m), 3.1 - 3.7 (4H, m), 4.22 (lH, m), 6.17, 6.19 (Total 2H, each d, J = 8.57 Hz), 6.53, 6.56 (Total 2H, each d, J = 8.57 Hz), 7.1 - 7.4 (5H, m), 7.57, 7.59 (Total lH, each t, J = 8.28 Hz), 8.12 (1H, d, J = 8.28 Hz), 8.15 - 8.35 (2H, m), 8.53 (1H, dd, J = 6.28, 1.0 Hz), 9.18 (1H, s).
Example 35. 1-rN-(tert-Butoxvcarbonvl)-p-nitro- phenolalans11-4-(p-methoxvPhenvl)piperazine 9.00 g of N-(tert-butoxycarbonyl)-p-nitrophenylalanine was dissolved in 120 ml of tetrahydrofuran, 100 ml of methylene chloride and 100 ml of chloroform, and to the solution were sequentially added 7.68 g of N-(p-methoxyphenyl) piperazine dihydrochloride and 4.44 g of N-hydroxybenzotriazole monohydrate as well as 20 ml of triethylamine and 6 g of DCC, and the mixture was stirred at a room temperature for 18 hours.
The reaction mixture was concentrated to one third of the original volume under a reduced pressure, and after adding 200 ml of 2.5% potassium carbonate aqueous solution, extracted twice with 200 ml of chloroform.
The extract was dried over magnesium sulfate and concentrated under a reduced pressure to crystallize a product, which was washed with methanol to obtain 10.75 g of the title compound as colorless crystals.
1H-NMR (CDC13 , 6 ppm): 1.40 (9H, s), 2.73 (1H, m), 2.87 - 3.00 (3H, m), 3.04 (1H, dd, J = 6.3, 13.2 Hz), 3.17 (lH, dd, J = 7.3, 13.2 Hz), 3.35 (lH, m), 3.55 - 3.70 (2H, m), 3.77 (3H, s), 3.84 (1H, m), 4.92 (1H, m), 5.4 (lH, d, J = 8.8 Hz), 6.83 (4H, s), 7.38 (2H, d, J = 8.8 Hz), 8.16 (2H, d, J = 8.8 Hz).
Reference Example 36. 1- FN- (tert-Butoxycarbonyl - p-aminophenylalanyl 1-4- (p-methoxvphenyl piperazine 10.75 g of the crystals obtained in Reference Example 35 was dissolved in a mixed solvent of 100 ml of tetrahydrofuran and 20 ml of methanol, and to the solution was added 5 g of 5% palladium on carbon, and the mixture was stirred for 2 hours at a room temperature in a hydrogen atmosphere. After filtering off insoluble matter, the filtrate was concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (200:1 to 100:1), to obtain 10.08 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.43 (9H, s), 2.42 (lH, m), 2.75 - 3.00 (4H, m), 3.13 (lH, m), 3.43 (1H, m), 3.57 (lH, m), 3.63 - 3.73 (2H, m), 3.76 (3H, s), 4.78 (1H, m), 5.43 (1H, br), 6.59 (2H, d, J = 8.3 Hz), 6.82 (4H, s), 6.98 (2H, d, J = 8.3 Hz).
Reference Example 37. 1-F 3-(p-Aminophenyl)-2- (tert-butoxycarbonylamino propyl 1-4- (p-methoxy- phenyl piperazine 2.54 g of lithium aluminum hydride was suspended in 75 ml of tetrahydrofuran, to the suspension was added a solution of 8.91 g of aluminum chloride in 75 ml of ether with ice cooling, and also a solution of 10.08 g of the amorphous compound obtained in Reference Example 36 in 100 ml of tetrahydrofuran was added dropwise for 20 minutes with ice cooling. Under the same condition the mixture was stirred for one hours, and after terminating the reaction by adding a small amount of water, 70 ml of 30% potassium carbonate aqueous solution and 200 ml of chloroform were added to the reaction mixture, which was then filtered to remove insoluble matter using silica gel as a filter aid. The insoluble matter was washed with 20% methanol in chloroform, and the filtrates were combined and concentrated under a reduced pressure. 200 ml of saturated sodium bicarbonate aqueous solution was added to the residue, the mixture was extracted twice with 100 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure.
Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 7.72 g of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 1.43 (9H, s), 2.30 (2H, d, J = 6.8 Hz), 2.48 - 2.68 (4H, m), 2.78 (2H, t, J = 6.3 Hz), 3.06 (4H, t, J = 4.9 Hz), 3.76 (3H, s), 3.86 (1H, m), 4.59 (lH, br), 6.62 (2H, d, J = 8.3 Hz), 6.82 (2H, d, J = 9.3 Hz), 6.89 (2H, d, J = 9.3 Hz), 6.98 (2H, d, J = 8.3 Hz).
Reference Example 38. 1-F 2- (tert-Butoxycarbonyl- amino-3-( p-phthalimidephenol )protyl 1-4- p-methoxy- phenyl piperazine 7.0 g of the amorphous compound obtained in Reference Example 37 was dissolved in 70 ml of chloroform, to the solution was added 2.66 g of phthalic anhydride. The mixture was refluxed for one hour, concentrated under a reduced pressure, and after an addition of 100 ml of toluene, further ref luxed for 2 hours. The solvent was evaporated off under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1), to obtain 8.91 g of the title compound as colorless crystals.
1H-NMR (CDC13 , s ppm): 1.45 (9H, s), 2.37 (2H, d, J = 6.8 Hz), 2.51 - 2.71 (4H, m), 2.96 (2H, d, J = 5.4 Hz), 3.09 (4H, t, J = 4.9 Hz), 3.77 (3H, s), 4.01 (1H, m), 4.66 (1H, br), 6.83 (2H, d, J = 9.3 Hz), 6.90 (2H, d, J = 9.3 Hz), 7.36 (4H, s), 7.79 (2H, dd, J = 3.4, 5.4 Hz), 7.96 (2H, dd, J = 3.4, 5.4 Hz).
Example 125. N-{2-[4-(p-Methoxyphenyl)pipera- zinyl]-1-(p-Phthalimidebenzyl)ethyl}-5-isoqui- nolinesulfonamide 8.91 g of the crystals obtained in Reference Example 38 was dissolved in 50 ml of ethyl acetate, to the solution was added 100 ml of 4 N hydrogen chloride in ethyl acetate, and the mixture was stirred at a room temperature for 1 hour. The reaction mixture was concentrated under a reduced pressure, and to the residue was added 200 ml of saturated sodium bicarbonate aqueous solution, and the mixture was extracted twice with 100 ml of 20% methanol/chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure to crystallize an amino-free compound.
The crystals was suspended in 120 ml of tetrahydrofuran, to the suspension were added 5.0 g of 5-isoquinolinesulfonyl chloride.HCl and 20 ml of triethylamine with ice cooling, and after warming to a room temperature, the mixture was stirred for 2 hours. After adding 200 ml of water, formed crystals was collected. The filtrate was extracted twice with 100 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated to dryness under a reduced pressure to obtain a residue. The residue with the crystals was sequentially washed with methanol, ethyl acetate and hexane to obtain 6.49 g of the title compound as colorless crystals.
Melting point: 204 - 2110C (decomposed); IR (KBr) cm 1 1710, 1510, 1370, 1235, 1150, 1130; H-NMR (CDC13 , 6 ppm): 2.03 - 2.33 (6H, m), 2.46 - 2.59 (2H, m), 2.59 - 2.72 (2H, m), 2.85 (1H, dd, J = 7.3, 13.7 Hz), 3.10 (1H, dd, J = 4.4, 13.7 Hz), 3.41 (1H, m), 3.77 (3H, s), 5.63 (1H, br), 6.73 (2H, d, J = 9.3 Hz), 6.83 (2H, d, J = 9.3 Hz), 7.20 (2H, d, J = 8.8 Hz), 7.29 (2H, d, J = 8.8 Hz), 7.74 (1H, t, J = 8.3 Hz), 7.80 (2H, dd, J = 3.4, 5.4 Hz), 7.96 (2H, dd, J = 3.4, 5.4 Hz), 8.24 (lH, d, J = 8.3 Hz), 8.48 - 8.52 (2H, m), 8.72 (1H, d, J = 6.4 Hz), 9.36 (1H, s).
Example 126. N-{2-F4- (p-Methoxyphenyl)pipera- zinyll-1-(p-phthalimidebenzol)ethvlT-N-meths1-5- isopuinolinesulfonamide 4.71 g of the crystals obtained in Example 125 was dissolved in 70 ml of dimethylformamide, to the solution were sequentially added 500 mg of 60% sodium hydride and 1 ml of methyl iodide with ice cooling, and the mixture was stirred under the same condition for 3 hours. The reaction was terminated by adding a small amount of water, and after adding 200 ml of saturated ammonium chloride aqueous solution, the mixture was extracted twice with 100 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure.To resulting residue were added 50 ml of acetic anhydride and 1.2g of sodium acetate, and the mixture was stirred for one hour at 800C and then concentrated to dryness under a reduced pressure, and resulting residue was dissolved in 200 ml of ethyl acetate. The solution was sequentially washed with 100 ml of saturated sodium bicarbonate aqueous solution and 100 ml of saturated sodium chloride aqueous solution, dried over magnesium sulfate, and concentrated under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 4.84 g of the title compound as colorless crystals.
Melting point: 170 - 1720C; IR (KBr) cm 1 1710, 1610, 1510, 1375, 1300, 1235, 1145, 1125; 1H-NMR (CDC13 , 6 ppm): 2.48 (1H, dd, J = 7.3, 13.2 Hz), 2.50 - 2.63 (4H, m), 2.66 (lH, dd, J = 7.3, 13.2 Hz), 2.82 (1H, dd, J = 6.8, 14.2 Hz), 2.86 - 2.96 (4H, m), 2.97 (3H, s), 3.02 (lH, dd, J = 6.8, 14.2 Hz), 3.77 (3H, s), 4.32 (lH, m), 6.84 (4H, s), 7.15 (2H, d, J = 8.8 Hz), 7.22 (2H, d, J = 8.8 Hz), 7.61 (lH, t, J = 7.3 Hz), 7.81 (2H, dd, J = 2.9, 5.4 Hz), 7.97 (2H, dd, J = 2.9, 5.4 Hz), 8.13 (lH, d, J = 8.3 Hz), 8.31 (2H, d, J = 6.4 Hz), 8.60 (1H, a, J = 6.3 Hz), 9.23 (lH, s).
Example 127.
1.5 g of the crystals obtained in Example 125 was suspended in 30 ml of ethanol, to the suspension was added 3 ml of hydrozine hydrate, and the mixture was refluxed for one hour. After adding 10% sodium hydroxide aqueous solution, the reaction mixture was extracted twice with 30 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under reduced pressure to form crystals, which was washed with a mixed solvent of ethyl acetate and methanol, to obtain 1.14 g of N-{1-(p-aminovenzyl)-2-t4-(p-methoxyphenyl) -piperazinyl]ethyl}-5-isoquinolinesulfonamide as light yellow crystals.
Melting point: 210 - 2110C; IR (RBr) cm : 1615, 1510, 1330, 1245, 1225, 1150, 1130, 1025; 1H-NMR (CDC13 , 6 ppm): 2.12 - 2.34 (6H, m), 2.53 - 2.72 (5H, m), 2.85 (1H, dd, J =4.9, 14.2 Hz), 3.31 (1H, m), 3.52 (2H, br), 3.77 (3H, s), 5.48 (lH, br), 6.43 (2H, d, J = 8.3 Hz), 6.75 (2H, d, J = 9.3 Hz), 6.77 (2H, d, J = 8.3 Hz), 6.83 (2H, d, J = 9.3 Hz), 7.70 (lH, t, J = 7.8 Hz), 8.20 (lH, d, J = 8.3 Hz), 8.44 (.lH, d, J = 6.4 Hz), 8.47 (lH, dd, J = 1.0, 7.3 Hz), 8.68 (1H, d, J = 6.4 Hz), 9.35 (1H, s).
Example 128.
4.64 g of the crystals obtained in Example 126 was suspended in 80 ml of ethanol, to the suspension 8 ml of hydrazine hydrate was added, and the mixture was refluxed for 90 minutes. After adding 100 ml of 10% sodium hydroxide, the reaction mixture was extracted twice with 80 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1), to obtain 3.29 g of N-{1-(p-amino benzyl)-2-[4-(p-methoxyphenyl)piperazinyl]ethyl}-N- methyl-5-isoquinolinesulfonamide in a yellow amorphous form.
IR (KBr) cm : 1620, 1510, 1315, 1235, 1150, 1125; 1H-NMR (CDC13 , E ppm): 2.43 (1H, dd, J = 6.8, 13.2 Hz), 2.53 - 2.66 (6H, m), 2.85 (1K, dd, J = 6.4, 14.2 Hz), 2.87 - 2.94 (4H, m), 2.92 (3H, s), 3.50 (2H, br), 3.77 (3H, s), 4.20 (lH, m), 6.34 (2H, d, J = 8.3 Hz), 6.75 (2H, d, J = 8.3 Hz), 6.84 (4H, s), 6.56 (lH, t, J = 7.3 Hz), 8.09 (lH, d, J = 8.3 Hz), 8.24 (lH, d, J = 6.3 Hz), 8.31 (lH, dd, J = 1.0, 7.3 Hz), 8.56 (lH, d, J = 5.9 Hz), 9.25 (lH, d, J = 1.0 Hz).
Example 129.
500 mg of the amorphous compound obtained in Example 128 was dissolved in 5 ml of pyridine, to the solution was added 305 mg of 5-isoquinolinesulfonyl chloride.HCl under ice cooling, and the mixture was stirred under the same condition for 20 minutes and then at a room temperature for one hour. After adding 30 ml of saturated sodium bicarbonate aqueous solution, the reaction mixture was extracted twice with 20 ml of chloroform, and the extract was dried over magnesium sulfate and concentrated under a reduced pressure.
Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (50:1), to obtain 665 mg of N-{l-[p-(S-isoquinolinesulfonylamin6benzyl) ]-2-[4-(p- methoxyphenyl)piperazinyl]ethyl}-N-methyl-5-iso- quinolinesulfonamide in a yellow amorphous form.
IR (KBr) cm 1 1615, 1510, 1325, 1225, 1150, 1130; 1H-NMR (CDC13 , 6 ppm): 2.34 (lH, dd, J = 7.3, 12.7 Hz), 2.45 - 2.61 (6H, m), 2.79 - 2.94 (5H, m), 2.90 (3H, s), 3.77 (2H, s), 4.04 (lH, m), 6.55 (2H, d, J = 8.3 Hz), 6.70 (2H, d, J = 8.3 Hz), 6.83 (4H, s), 7.57 (2H, t, J = 7.8 Hz), 8.08 - 8.15 (3H, m), 8.28 - 8.35 (2H, m), 8.40 (lH, d, J = 6.4 Hz), 8.50 (1H, d, J = 5.3 Hz), 8.64 (1H, d, J = 6.4 Hz), 9.29 (1H, s), 9.31 (1H, d, J = 1.0 Hz).
Example 130.
200 mg of the crystals obtained in Example 127 was dissolved in 3 ml of pyridine, to the solution was added 130 mg of 5-isoquinolinesulfonyl chloride.l/2 sulfate with ice cooling, and the mixture was stirred with ice cooling for 20 minutes and then at a room temperature for one hour, and after adding 30 ml of saturated sodium bicarbonate aqueous solution, extracted twice with 20 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (50:1 to 25:1), to obtain 270 mg of N-(l-[p-(5-isoquinoline- sulfonylaminobenzyl)]-2-[4-(p-methoxyphenyl)pipera- zinyl]ethyl)-5-isoquinolinesulfonamide in a yellow amorphous form.
IR (KBr) cam : 1615, 1505, 1330, 1230, 1150, 1125; 1H-NMR (CDC13 , s ppm): 2.16 - 2.33 (6H, m), 2.49 - 2.81 (6H, m), 3.28 (lH, m), 3.76 (3H, s), 6.69 (2H, d, J = 8.3 Hz), 6.73 (2H, d,.J = 9.3 Hz), 6.79 (2H, d, J = 8.3 Hz), 6.82 (2H, d, J = 9.8 Hz), 7.61 (lH, t, J = 7.8 Hz), 7.67 (lH, t, J = 7.8 Hz), 8.16 (lH, d, J = 8.3 Hz), 8.19 (1H, d, J = 8.3 Hz), 8.34 - 8.48 (4H, m), 8.62 (2H, d, J = 6.4 Hz), 9.33 (1H, s), 9.35 (1H, s).
Example 131. N-F F l-;p-FN-S-Isoauinolinesulfonyl)-N- (methvlamino)benzvllT-2- r 4-(D-methoxvDhenolsipera- zinvlletholll-N-methYl-5-isoauinolinesulfonamide 503 mg of the amorphous compound obtained in Example 129 was dissolved in 8 ml of dimethylformamide, to the solution were added 50 mg of 60% sodium hydride and 0.1 ml of methyl iodide with ice cooling, and the mixture was stirred for one hours with ice cooling.
After adding 30 ml of saturated sodium chloride aqueous solution, the reaction mixture was extracted with 30 ml of ethyl acetate, and the extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate and concentrated under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1), to obtain 488 mg of the title compound in a yellow amorphous form.
IR (KBr) cm 1 1610, 1505, 1340, 1320, 1235, 1145, 1125,; 1H-NMR (CDC13 , s ppm): 2.32 (1H, dd, J = 6.4, 13.2 Hz), 2.41 - 2.56 (5H, m), 2.73 - 2.98 (6H, m), 2.88 (3H, s), 3.23 (3H, s), 3.77 (3H, s), 4.31 (1H, m), 6.82 (4H, s), 6.89 (2H, d, J = 8.3 Hz), 7.01 (2H, d, J = 8.3 Hz), 7.63 (1H, t, J = 7.8 Hz), 7.64 (1H, t, J = 7.8 Hz), 8.02 (1H, d, J = 5.9 Hz), 8.13 (1H, d, J = 8.3 Hz), 8.19 (1H, d, J = 8.3 Hz), 8.23 (1H, d, J = 7.3 Hz), 8.34 (1H, d, J = 6.3 Hz), 8.40 - 8.47 (2H, m), 8.60 (1H, d, J = 5.9 Hz), 9.28 (1H, s), 9.29 (1H, s).
ExamPle 132. N-l-Tp-/4-Picolvloxv)benzvll-2-T4- (2-pyrimidyl piperazinyl lethyl -N-methyl-S-iso- quinolinesulfonamide 100 mg of the amorphous compound obtained in Example 42 was dissolved in 10 ml of a mixture of dried tetrahydrofuran/dried dimethylformamide (1:1), to the solution were added 34.8 mg of 4-picolyl chloride hydrochloride and then 24 mg of triethylamine, and the mixture was stirred at a room temperature for 30 minutes. After adding 10 mg of 60% sodium hydride, the mixture was stirred overnight at a room temperature, and after adding 20 g of water, extracted twice with 20 ml of chloroform. The extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate, and evaporated to remove the solvent under a reduced pressure. The resulting residue was applied to a silica gel column and extracted with chloroform/methanol (100:1) to obtain 73 mg of the title compound in colorless amorphous form.
NMR (CDC13) E ppm: 2.45 (4H, complex), 2.5 - 2.75 (2H, complex), 2.95 (3H, s), 3.65 (4H, complex), 4.22 (1H, complex), 5.0 (2H, s), 6.49 (1H, t, J = 4.26 Hz), 6.6 (2H, d, J = 8.0 Hz), 6.9 (2H, d, J = 8.0 Hz), 7.4 (2H, brd), 7.6 (1H, t, J = 8.3 Hz), 8.11 (1H, d, J = 8.3 Hz), 8.23 (1H, d, J = 6.64 Hz), 8.3 (2H, d, J = 4.26 Hz), 8.37 (1H, dd, J = 1.0, 6.6 Hz), 8.57 (1H, d, J = 6.6 Hz), 8.65 (2H, brd), 9.25 (1H, d, J = 1.0 Hz).
Example 133. N-rl-Tp-(2-Picolvloxvbenzvl-2-r4- (2-pyrimidyl )piperazinyl 1 ethyl-N-methyl-S-iso- quinolinesulfonamide The same procedure as described in Example 132 was repeated except that the same amount of 2-picolyl chloride hydrochloride was used in place of 4-picolyl chloride hydrochloride, to obtain 74.4 mg of the title compound in colorless amorphous form.
NMR (CDC13) E ppm: 2.45 (4H, complex), 2.5 - 2.9 (2H, complex), 2.98 (3H, s), 3.75 (4H, complex), 4.2 (1H, complex), 5.13 (2H, s), 6.46 (1H, t, J = 4.8 Hz), 6.65 (2H, d, J = 8.0 Hz), 6.9 (2H, d, J = 8.0 Hz), 7.15 (lH, complex), 7.58 (2H, t, J = 6.9 Hz), 7.75 (1H, complex), 8.1 (1H, d, J = 8.0 Hz), 8.2 - 8.35 (2H, complex), 8.3 (1H, d, J = 4.8 Hz), 8.58 (1H, d, J = 6.6 Hz), 8.6 (1H, brs), 9.25 (1H, s).
Example 134. N-;l-Fp-(4-Picolyloxy)benzyll-2-r4- (2-pvridol!piperazinyllethylT-N-metho1-5-iso- auinolinesulfonamide The same procedure as described in Example 132 was repeated except that the product of Example 46 was used in place of the amorphous compound obtained in Example 42, to obtain the title compound in a colorless amorphous form in a yield of 53.5%.
NMR (CDC13) s ppm: 2.5 (4H, complex), 2.5 - 2.75 (2H, complex), 2.95 (3H, s), 3.38 (4H, complex), 4.22 (1H, complex), 5.0 (2H, s), 6.58 (2H, d, J = 8.6 Hz), 6.6 (2H, t, J = 5.7 Hz), 6.9 (2H, d, J = 8.6 Hz), 7.35 7.5 (4H, complex), 7.58 (lah, t, J = 7.8 Hz), 8.07 (lH, d, J = 8.1 Hz), 8.17 (1H, brd), 8.23 (lH, d, J = 6.1 Hz), 8.35 (1H, dd, J = 1.0, 7.4 Hz), 8.57 (lH, d, J = 6.1 Hz), 8.63 (1H, brd), 8.63 (1H, d, J = 5.8 Hz), 9.2 (1H, d, J = 1.0 Hz).
Example 135. N-l-(p-(2-Picolvloxvbenzvll-2-r4- (2-psridyl)piperazinylZethvlT-N-methvl-5-iso- quinolinesulfonamide The same procedure as described in Example 133 was repeated except that the product of Example 46 was used in place of the amorphous compound obtained in Example 42, to obtain the title compound in a colorless amorphous for in a yield of 59.5%.
NMR (CDC13) 6 ppm: 2.5 (4H, complex), 2.5 - 2.9 (2H, complex), 2.95 (3H, s), 3.38 (4H, complex), 4.22 (lH, complex), 5.12 (2H, s), 6.55 - 6.65 (2H, complex), 6.54 (2H, d, J = 8.6 Hz), 6.9 (2H, d, J = 8.6 Hz), 7.2 7.25 (1H, complex), 7.4 - 7.7 (4H, complex), 7.65 - 7.8 (lH, complex), 8.1 (lH, d, J = 7.7 Hz), 8.2 (lH, brd), 8.27 (1H, d, J = 6.6 Hz), 8.3 (lH, d, J = 6.6 Hz), 8.57 (1H, d, J = 6.3 Hz), 8.6 (1H, brs), 9.73 (1H, s).
Example 136. N-(2-Aminoethol}-N- r 2-(4-benzvloxo- carbonslpiperazinyl)-1-{p-methoxYbenzol)etholl-5- isoquinolinesulfonamide 1.0 g of the product obtained in Example 73 was dissolved in 5 ml of tetrahydrofuran, to the solution were added 685 mg of triphenylphosphine and 340 mg of N-tert-butoxycarbonylethanolamine, and then added dropwise a solution of 530 mg of diisopropyl azodicarboxylate in 3 ml of tetrahydrofuran with stirring in a ice bath. After removing from the ice bath, the mixture was stirred at a room temperature for 3 hours and poured to water, and the mixture was alkalized with sodium bicarbonate and extracted twice with 150 ml of chloroform. The extract was dried over magnesium sulfate and the solvent was evaporated off under a reduced pressure.Resulting oil was dissolved in 2 ml of ethyl acetate, to the solution was added 30 ml of 4 N hydrochloric acid in ethyl acetate, and the mixture was stirred at a room temperature for 30 minutes. After adding 100 ml of 1 N hydrochloric acid, the reaction mixture was washed twice with ethyl acetate, and the aqueous layer was alkalized with sodium Obicarbonate and extracted twice with 150 ml of chloroform. The extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, and resulting oil was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1) to obtain 400 mg of the title compound in colorless amorphous form.
IR (KBr) cm 1 1701, 1514, 1325, 1248, 1135, 763, 601; NMR (CDC13) 6 ppm: 1.99 (2H, brs), 2.15 - 2.40 (5H, m), 2.55 - 2.80 (3H, m), 2.90 - 3.10 (2H, m), 3.20 - 3.70 (6H, m), 3.73 (3H, s), 4.98 (lH, m), 5.10 (2H, s), 6.54 (2H, d, J = 8.55 Hz), 6.77 (2H, d, J = 8.55 Hz), 7.33 (5H, s), 7.62 (lH, dd, J = 8.06, 7.57 Hz), 8.14 (lH, d, J = 8.06 Hz), 8.34 (lH, d, J = 6.10 Hz), 8.39 (lH, d, J = 7.57 Hz), 8.63 (lH, d, J = 6.10 Hz), 9.28 (lH, s).
Example 137. N-T2-(4-Benzvloxvcarbonvlpiperazinvl- l-p-methoxybenzvl)ethyl1-N-(2-dimetholaminoethYl!-- 5-isoquinolinesulfonamide 6.08 g of the product obtained in Example 73 was dissolved in 30 ml of tetrahydrofuran, to the solution were added 5.0 g of triphenylphosphine and 1.42 g of N,N-dimethylethanolamine, and then added dropwise a solution of 3.21 g of diisopropyl azodicarboxylate in 10 ml of tetrahydrofuran with stirring in ice bath.
After removing from the ice bath, the reaction mixture was stirred at a room temperature for 3 hours, diluted with ethyl acetate, and extracted with 100 ml of 1 N hydrochloric acid. The extract was alkalized with sodium bicarbonate and extracted twice with 100 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting oil was applied to a silica gel column and eluted with chloroform/methanol (200:1 to 100:1), to obtain 4.99 g of the title compound in a colorless amorphous form.
cm1 IR (KBr) cm : 1703, 1514, 1327, 1247, 1135, 600; NMR (CDC13) 6 ppm: 2.10 - 2.45 (5H, m), 2.26 (6H, s), 2.45 - 2.85 (SH, m), 3.20 - 3.65 (6H, m), 3.73 (3H, s), 4.00 (1H, m), 5.10 (2H, s), 6.53 (2H, d, J = 8.79 Hz), 6.83 (2H, d, J = 8.79 Hz), 7.34 (5H, s), 7.56 (1H, dd, J = 8.05, 7.57 Hz), 8.10 (1H, d, J = 8.05 Hz), 8.31 (1H, d, J = 6.10 Hz), 8.35 (lH, d, J = 7.57 Hz), 8.59 (1H, d, J = 6.10 Hz), 9.25 (lH, s).
Example 138. N-(2-Acetoxsethol)-N- r 2-(4-benzoloxv- carbonvlDiperazinvl)-l-(P-methoxybenzvl)ethvll-5- isoquinolinesulfonamide.
1.0 g of the product obtained in Example 73 was dissolved in 5 ml of tetrahydrofuran, to the solution were added 220 mg of ethylene glycol monoacetate and 685 mg of triphenylphosphine in place of N-tert-butoxycarbonylethanolamine, according to the procedure described in Example 136, to obtain 600 mg of the title compound in a colorless amorphous form.
NMR (CDC13) 6 ppm: 2.04 (3H, s), 2.20 - 2.45 (5H, m), 2.60 - 2.80 (3H, m), 3.20 - 3.40 (4H, m), 3.45 3.73 (2H, m), 3.74 (3H, s), 4.04 (1H, m), 4.27 (2H, t, J = 6.84 Hz), 5.10 (2H, s), 6.54 (2H, d, J = 8.55 Hz), 6.82 (2H, d, J = 8.55 Hz), 7.34 (5H, s), 7.59 (lH, dd, J = 8.05, 7.57 Hz), 8.13 (lH, d, J = 8.05 Hz), 8.30 (lH, d, J = 6.10 Hz)-, 8.36 (1H, d, J = 7.57 Hz), 9.27 (1H, s).
Example 139. N- F 2- (4-Benzyloxycarbonylpipera- zinoll (p-methoxYbenzol!etholl-N-(2-hYdroxv- ethvl\-5-isoquinolinesulfonamide 600 mg of the amorphous compound obtained in Example 138 was dissolved in 6 ml of methanol and 3 ml of tetrahydrofuran, to the solution was added 6 ml of 1 N sodium hydroxide aqueous solution, and the mixture was stirred at a room temperature for 2 hours. The reaction mixture was diluted with water and extracted twice with 50 ml of chloroform, and the extract was washed with saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting oil was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1) to obtain 403 mg of the title compound in a colorless amorphous form.
IR (KBr) cm 1 1701, 1514, 1433, 1332, 1249, 1136; NMR (CDCl3) b ppm: 2.10 - 2.25 (3H, m), 2.25 2.50 (4H, m), 2.50 - 2.70 (lH, m), 3.10 - 3.45 (5H, m), 3.55 - 3.75 (2H, m), 3.76 (3H, s), 4.00 - 4.20 (2H, m), 5.08 (2H, s), about 5.4 (lH, br), 6.70 (2H, d, J = 8.79 Hz), 6.79 (2H, d, J = 8.79 Hz), 7.32 (5H, s), 7.73 (lH, dd, J - 8.30, 7.32 Hz), 8.22 (lH, d, J = 8.3 Hz), 8.50 (1H, d, J = 7.32 Hz), 8.63 (1H, d, J = 6.10 Hz), 8.72 (1H, d, J = 6.10 Hz), 9.34 (1H, s).
Example 140. N-r2-r4-(3,4-Dichlorobenzvlamino)- piperidinol-1-(p-methoxybenzyl ! etholE-N-methyl-5- isoquinolinesulfonamide The amorphous compound obtained in Example 94 was subjected to alkaline hydrolysis, methylation with methyl iodide and potassium carbonate in dimethylformamide/tetrahydrofuran (1:1), and reflux with 3 N hydrochloric acid, to obtain N-{1-(p-methoxybenzyl) 2- ( 4-oxopiperidino ) ethyl}-N-methyl-S-isoquinolinesulfon- amide in a colorless amorphous form.
NMR (CDC13) 6 ppm: 2.37 (4H, t, J = 5.99 Hz), 2.40 - 2.90 (8H, m), 2.94 (3H, s), 3.74 (3H, s), 4.23 (lH, m), 6.51 (2H, d, J = 8.55 Hz), 6.83 (2H, d, J = 8.55 Hz), 7.55 (1H, dd, J = 8.32, 7.50 Hz), 8.10 (lH, d, J = 8.32 Hz), 8.19 (1H, d, J = 7.50 Hz), 8.19 (lH, d, J = 6.10 Hz), 8.55 (1H, d, J = 6.10 Hz), 9.25 (lH, s).
3.34 g of this compound was dissolved in 30 ml of methanol, to the solution were added 1.89 g of 3,4-dichlorobenzylamine and 0.6 ml of acetic acid, and the mixture was stirred at a room temperature for 3 hours. The reaction mixture was cooled in a ice bath, and after adding 450 mg of sodium cyanoborohydride, stirred with ice cooling for 30 minutes and then at a room temperature for one hour. This reaction mixture was alkalized with sodium bicarbonate and extracted twice with 150 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting oil was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1), to obtain 2.78 g of the title compound in a colorless amorphous form.
IR (KBr) cm 1 1514, 1327, 1249, 1157, 1130, 826, 600; NMR (CDC13) 6 ppm: 1.05 - 1.40 (2H, m), 1.60 2.15 (4H, m), 2.30 - 2.90 (8H, m), 2.93 (3H, s), 3.73 (5H, s), 4.13 (lH, m), 6.49 (2H, d, J = 8.79 Hz), 6.83 (2H, d, J = 8.79 Hz), 7.15 (1H, dd, J = 8.20, 1.95 Hz), 7.38 (1H, d, J = 8.20 Hz), 7.44 (lH, d, J = 1.95 Hz), 7.56 (1H, dd, J = 8.06, 7.57 Hz), 8.08 (1H, d, J = 8.06 Hz), 8.19 (lH, d, J = 6.35 Hz), 8.29 (lH, d, J = 7.57 Hz), 8.55 (lH, d, J = 6.35 Hz), 9.23 (lH, s).
Example 141. N-F2-F4-FN-(3 ,4-Dichlorobenzyl)-N- methylamino 1 piperidino-1- (p-methoxybenzvl ethyl 11 - N-methyl-5-isoauinolinesulfonamide 1.62 g of the amorphous compound obtained in Example 140 was dissolved in 10 ml of tetrahydrofuran and 10 ml of dimethylformamide, to the solution was added 115 mg of 60% sodium hydride with stirring under ice cooling, and the mixture was allowed to react at the same temperature for 5 minutes and then at a room temperature for 15 minutes and again ice-cooled. After adding 405 mg of methyl iodide, the mixture was allowed to react at the same temperature for 5 minutes and then at a room temperature for 2 hours, and poured to water.
The mixture was extracted with 200 ml of ethyl acetate, and the extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. Resulting oil was applied to a silica gel column and eluted with chloroform/methanol (200:1 to 100:1) to obtain 880 mg of the title compound in a colorless amorphous form.
IR (KBr) cm 1 1514, 1329, 1249, 1157, 1131, 826, 600; NMR (CDCl3) 6 ppm: 1.10 - 2.10 (6H, m), 2.14 (3H, s), 2.20 - 3.00 (7H, m), 2.93 (3H, s), 3.46 (2H, s), 3.73 (3H, s), 4.12 (1H, m),6.51 (2H, d, J = 8.55 Hz), 6.85 (2H, d, J = 8.55 Hz), 7.15.(1H, dd, J = 8.30, 1.71 Hz), 7.37 (1H, d, J = 8.30 Hz), 7.42 (1H, d, J = 1.71 Hz), 7.56 (lH, t, J = 7.82 Hz), 8.08 (1H, d, J = 7.82 Hz), 8.20 (1H, d, J = 6.11 Hz), 8.30 (1H, d, J = 7.82 Hz), 8.56 (lH, d, J = 6.11 Hz), 9.23 (1H, s).
Reference Example 39. 4-Chlorocinnamyl alcohol 25.9 g of p-chlorocinnamic acid was dissolved in 250 ml of methanol, to the solution was added 1.5 ml of concentrated sulfuric acid, and the mixture was refluxed for 2 hours. The reaction mixture was poured on ice, and the mixture was alkalized with sodium bicarbonate and extracted twice with 1000 ml of chloroform. The extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (10:1), to obtain 26.5 g of the methyl p-chlorocinnamate.
This compound was dissolved in 250 ml of toluene, to the solution was added 200 ml of 1.5 M diisobutyl aluminum hydride in toluene with stirring under ice cooling, and the mixture was stirred for 2 hours. The reaction mixture was poured on ice, acidified with concentrated hydrochloric acid, and extracted twice with 700 ml of benzene. The extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (4:1), to obtain 21.0 g of the title compound as colorless crystals.
1H-NMR (CDC13 , 6 ppm): 4.33 (2H, brs), 6.33 (1H, dt, J = 17.1, 5.7 Hz), 6.59 (lH, dt, J = 17.1, 2.0 Hz), 7.29 (4H, s).
Reference Example 40. N-4-Chlorocinnamyl-1,2- phenylenediamine 11.9 g of the crystals obtained in Reference Example 39 was dissolved in 120 .ml of chloroform, to the solution was added 10.1 g of thionyl chloride with stirring in an ice bath, and after removing from the ice bath, the mixture was stirred for one hour while allowing the reaction temperature to rise up to a room temperature. Chloroform and excess thionyl chloride were evaporated off under a reduced pressure, to the residue was added benzene, and the solvent was evaporated off under a reduced pressure. Resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (15:1), to obtain 11.3 g of 4-chlorocinnamyl chloride as colorless crystals.
1H-NMR (CDCl3 , s ppm): 4.23 (2H, dd, J = 6.3, 1.0 Hz), 6.29 (1H, dt, J = 16.6, 6.9 Hz), 6.62 (1H, dt, J = 16.6, 1.0 Hz), 7.30 (4H, s).
19.6 g of 1,2-phenylenediamine was dissolved in 300 ml of dimethylformamide, to the solution were added 11.3 g of the above-prepared 4-chlorocinnamyl chloride crystals and 12.5 g of potassium carbonate at a room temperature with stirring, and the mixture was stirred for 48 hours under the same condition. After adding water and sodium chloride, the reaction mixture was extracted twice with 1000 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure.
Resulting residue was applied to a silica gel column and eluted with hexane/ethyl acetate (3:1), to obtain 12.85 g of the title compound as colorless crystals.
1H-NMR (CDC13 , 6 ppm): 3.4 (3H, brs), 3.93 (2H, dd, J = 5.71, 1.0 Hz), 6.36 (lH, dt, J = 16.0, 5.71 Hz), 6.59 (1H, dt, J = 16.0, 1.0 Hz), 6.68 - 6.9 (4H, m), 7.28 (4H, s).
Example 142. N T N-r2-(4-Chlorocinnamvlamino)shenvll- 5-isoauinolinesulfonamide 12.85 g of the crystals obtained in Reference Example 40 was dissolved in 200 ml of pyridine, to the solution was added 15.1 g of 5-isoquinolinesulfonyl chloride, hydrochloride with stirring in a ice bath, and after removing from the ice bath, the mixture was allowed to react at a room temperature for 18 hours.
The reaction mixture was poured on ice, alkalized with sodium bicarbonate and extracted twice with 1000 ml of chloroform. The extract was dried over magnesium sulfate and the solvent was evaporated off under a reduced pressure to form scarcely soluble crystals. To the crystals was added chloroform, the whole was refluxed and then cooled, and the resulting crystals was collected by suction filtration, washed with chloroform and dried under a reduced pressure, to obtain 17.23 g of the title compound as colorless crystals.
Melting point: 205 - 2080C (decomposed); IR (KBr) cm : 1600, 1320, 1150, 1135; 1H-NMR (CDC13 + CD30D, S ppm): 3.73 (2H, dd, J = 5.62, 1.46 Hz), 6.04 (lH, dt, J = 15.8, 5.37 Hz), 6.27 6.35 (2H, m), 6.42 (1H, dt, J = 16.11, 1.46 Hz), 6.58 (lH, d, J = 7.81 Hz), 7.04 (lH, ddd, J = 8.30, 6.10, 2.93 Hz), 7.25 (2H, d, J = 9.03 Hz), 7.31 (2H, d, J = 9.03 Hz), 7.63 (lH, dd, J = 8.06, 7.33 Hz), 8.17 (lH, dd, J = 7.32 , 0.98 Hz), 8.30 (1H, dd, J = 7.57, 1.23 Hz), 8.47 (lH, dd, J = 6.35, 1.02 Hz), 8.55 (lH, d, J = 6.35 Hz), 9.25 (lH, d, J = 0.98 Hz).
Example 143. N-F 2- r 4 -Chlorocinnamvlamino phenyl 1 - N-methyl-S-isoguinolinesulfonamide 380 mg of the crystals obtained in Example 142 was dissolved in 6 ml of methanol, to the solution was added 10 ml of a solution of diazomethane in ether at a room temperature with stirring, and the mixture was stirred for 18 hours. The solvent was evaporated off under a reduced pressure to obtain an oil, which was then applied to a silica gel column and eluted with hexane/ethyl acetate (1:1) to obtain acetate, which was then recrystallized from hexane/ethyl acetate to obtain 270 mg of the title compound as colorless crystals.
Melting point: 149 - 1510C; IR (KBr) cm 1 1595, 1325, 1125, 830, 745; 1H-NMR (CDC13 , 6 ppm): 3.24 (3H, s), 3.87 (2H, m), 4.81 (1H, t, J = 5.71 Hz), 6.13 (1H, dt, J = 19.14, 5.71 Hz), 6.25 - 6.40 (2H, m), 6.53 (lH, dt, J = 19.14, 1.0 Hz), 6.67 (1H, d, J = 8.57 Hz), 7.05 - 7.18 (lH, m), 7.28 (4H, s), 7.67 (lH, t, J = 7.42 Hz), 8.19 (1H, d, J = 7.42 Hz), 8.28 (1H, d, J = 6.28 Hz), 8.32 (1H, dd, J = 7.42, 1.0 Hz), 8.51 (lH, d, J = 6.28 Hz), 9.30 (1H, d, J = 1.0 Hz).
Example 144. 1-(4-Chlorocinnamol)-4-(5-isoqui- nolinesulfonsl)-1,2,3,4-tetrahodroquinoxaline 5.0 g of the crystals obtained in Example 142 was dissolved in 75 ml of dimethylformamide, to the solution was added 4.6 g of potassium carbonate and 2.19 g of 1,2-dibromoethane at a room temperature with stirring, and the mixture was stirred for 60 hours. The reaction mixture was poured in water, saturated'with sodium chloride, and extracted twice with 400 ml of chloroform.
The extract was dried over magnesium sulfate, evaporated to remove the solvent undr a reduced prssure.
Resulting residue was applied to a silica gel column and eluted with chloroform/methanol (400:1) and then hexane/ethyl acetate (2:1), to obtain 3.32 g of the title compound in yellow amorphous form.
IR (KBr) cm 1 1600, 1340, 1150, 1130, 660; 1H-NMR (CDC13 , E ppm): 2.68 (2H, t, J = 5.71 Hz), 3.49 (2H, dd, J = 6.28, 1.0 Hz), 3.89 (2H, t, J = 5.71 Hz), 5.43 (1H, dt, J = 15.42, 6.28 Hz), 6.10 (1H, dt, J = 15.42, 1.0 Hz), 6.48 (1H, dd, J = 7.99, 1.0 Hz), 6.75 (1H, dt, J = 7.99, 1.0 Hz), 7.09 (2H, d, J = 7.99 Hz), 7.12 (lH, dt, J = 7.99, 1.0 Hz), 7.31 (2H, d, J = 7.99 Hz), 7.54 (1H, dd, J = 7.99, 1.0 Hz), 7.59 (IH, t, J = 7.99 Hz), 7.77 (1H, d, J = 6.28 Hz), 7.94 (1H, d, J = 7.99 Hz), 8.30 (1H, d, J = 6.28 Hz), 8.38 (1H, ad, J = 7.99, 1.0 Hz), 9.03 (1H, d, J = 1.0 Hz).
Example 145.
The same procedure as described in Example 142 was repeated except that N-[3-(3-pyridyl)allyl-1,2-phe- nylenediamine was used in place of N-(4-chlorocinnamyl)1,2-phenylenediamine, to obtain N-(2-[3-(3-pyridyl)a1- lylamino ] phenyl } -5-isoquinolinesulfonamide in a brown amorphous form.
1H-NMR (CDC13 , s ppm): 2.2 (lH, br), 3.78 (2H, dd, J = 5.14, 1.0 Hz), 4.85 (1H, br), 6.14 (1H, dt, J = 15.99, 5.14 Hz), 6.33 (2H, d, J = 4.57 Hz), 6.42 (1H, dt, J = 15.99, 1.0 Hz), 6.58 (1H, d, J = 7.42 Hz), 6.98 - 7.15 (1H, m), 7.26 (1H, dd, J = 7.42, 4.57 Hz), 7.59 (1H, t, J = 7.42 Hz), 7.65 (lH, dt, J = 7.42, 1.0 Hz), 8.16 (lH, d, J = 7.99 Hz), 8.30 (lH, d, J = 6.85 Hz), 8.35 - 8.53 (3H, m), 8.56 (lH, d, J = 6.28 Hz), 9.32 (lH, s).
Example 146.
The amorphous compound obtained in Example 145 was treated according to the procedure in Example 143-to obtain N-{2-[3-(3-pyridyl)allylamino)phenyl}-N-methyl -5-isoquinolinesulfonamide.
1H-NMR (CDC13 , 6 ppm): 3.24 (3H, s), 3.92 (2H, t, J = 4.57 Hz), 4.90 (1H, t, J = 5.71 Hz), 6.26 (1H, dt, J = 15.42, 5.14 Hz), 6.32 (2H, d, J = 4.57 Hz), 6.58 (1H, dt, J = 15.42, 1.0 Hz), 6.62 - 6.74 (2H, m), 7.05 - 7.20 (1H, m), 7.26 (1H, dd, J = 7.99, 4.57 Hz), 7.6 - 7.75 (1H, m), 8.21 (lH, d, J = 7.99 Hz), 8.28 (lH, d, J = 6.85 Hz), 8.32 (lH, d, J = 6.28 Hz), 8.47 (lH, dd, J = 5.71, 1.0 Hz), 8.51 (lH, d, J = 6.28 Hz), 8.58 (1H, d, J = 1.7 Hz), 9.31 (lH, s).
Reference Example 41. 2-Amino-3- (4-chlorocinnamyl- amino ! pyridine 7.71 g of p-chlorocinnamyl chloride and 13.5 g of 2,3-diaminopyridine were dissolved in 220 ml of dimethylformamide, and'to the solution was added 8.6 g of potassium carbonate, and the mixture was stirred at a room temperature for 50 hours, and after adding 300 ml of water, extracted twice with 200 ml of chloroform.
The extract was dried over magnesium sulfate and concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1), to obtain 4.52 g of the title compound as yellow crystals.
1H-NMR (CDC13 , 6 ppm): 3.38 (1H, br), 3.92 (2H, m), 4.20 (2H, br), 6.31 (lH, dt, J = 16.1, 5.9 Hz), 6.59 (lH, dt, J - 16.1, 1.5 Hz), 6.71 (1H, dd, J = 4.9, 7.8 Hz), 6.86 (1H, dd, J = 1.5, 7.8 Hz), 7.29 (4H, s), 7.63 (lH, dd, J = 1.5, 4.9 Hz).
Example 147. 3-(4-Chlorocinnamolamino)-2-(5-iso- cuinolinesulfonylamino pyridine 4.52 g of the crystals obtained in Reference Example 41 was dissolved in 50 ml of pyridine, to the solution were added 5.8 g of 5-isoquinolinesulfonyl chloride hydrochloride and 3 g of dimethylaminopyridine, and the mixture was stirred for 18 hours at a room temperature, after adding 150 ml of water, extracted twice with 80 ml of chloroform. The extract was dried over magnesium sulfate and concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1), and resulting crystals was washed with ethyl acetate, to obtain 1.2 g of the title compound as yellow crystals.
Melting point: 211 - 2170C (decomposed); IR (KBr) cm 1 1595, 1550, 1345, 1285, 1250, 1105; 1H-NMR (CDC13 , 6 ppm): 3.89 (2H, m), 5.45 (lH, t, J = 5.9 Hz), 6.12 (1H, dt, J = 16.1, 5.4 Hz), 6.45 (1H, d, J = 16.1 Hz), 6.51 - 6.62 (2H, m), 6.92 (1H, brs), 7.21 (2H, d, J = 8.8 Hz), 7.29 (2H, d, J = 8.8 Hz), 7.64 (1H, dd, J = 7.3, 8.3 Hz), 8.12 (lH, d, J = 8.3 Hz), 8.45 (1K, dd, J = 1.0, 7.3 Hz), 8.64 (1H, d, J = 5.9 Hz), 8.69 (1H, d, J = 5.9 Hz), 9.31 (1H, s).
Reference Example 42. Methvl 4-amino-3-(4-chloro cinnamylaminolbenzoate 5.0 g of methyl 3,4-diaminobenzoate was dissolved in 40 ml of dimethylformamide, and to the solution were added 2.07 g of potassium carbonate and 1.87 g of p-chlorocinnamyl chloride, and reaction was carried out according to the procedure in Reference Example 40, to obtain 2.0 g of the title compound as a light brown oil.
NMR (CDC13) 6 ppm: 3.85 (3H, s), 3.94 (2H, brd), 6.35 (1H, dt, J = 5.86, 15.8 Hz), 6.59 (1H, d, J = 5.8 Hz), 6.7 (lH, d, J = 8.02 Hz), 7.28 (4H, s), 7.4 (1H, d, J = 1.4 Hz), 7.46 (1H, dd, J = 1.4, 8.0 Hz).
Example 148. Methyl 4-(S-isopuinolinesulfonamino)- 3-(4-chlorocinnamolamino)benzoate 1.8 g of the oil obtained in Reference Example 42 was dissolved in 18 ml of pyridine, to the solution was added 1.29 g of 5-isoquinolinesulfonyl chloride hydrochloride with stirring under ice cooling, and the mixture was treated according to the procedure in Example 142 to obtain residue, which was then applied to a silica gel column and eluted with chloroform/methanol (100:1), to obtain'1.28 g of the title compound as light yellow crystals.
Melting point: 143 - 1450C (subliming at a higher temperature than melting point); NMR (CDC13) 6 ppm: 3.78 (2H, brd), 3.82 (3H, s), 6.0 (1H, dt, J = 5.86, 15.87 Hz), 6.4 (1H, d, J = 15.8 Hz), 6.45 (1H, d, J = 8.3 Hz), 7.05 (1H dd, J = 1.8, 8.3 Hz), 7.2 - 7.3 (5H, brs), 7.60 (1H, t, J = 7.6 Hz), 8.15 (1H, d, J = 8.3 Hz), 8.29 (1hut dd, J = 1.2, 7.3 Hz), 8.43 (1H, d, J = 6.1 Hz), 8.61 (1H, d, J = 6.1 Hz), 9.3 (1H, d, J = 1.2 Hz).
Reference Example 43. N-Cinnamyl-1,2-phenylene- diamine 3.24 g of ortho-phenylenediamine was dissolved in 30 ml of dimithylformaide, to the solution were added 2.07 g of potassium carbonate and 1.52 g of cinnamyl chloride, and the mixture was stirred overnight at a room temperature. After adding 100 ml of water, the reaction mixture was extracted twice with 100 ml and 50 ml of chloroform, and the extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform, to obtain 2.0 g of the title compound as light brown crystals.
Melting point: 59 - 660C (decomposed); NMR (CDC13) 6 ppm: 3.3 (2H, brs), 3.93 (2H, brd), 6.4 (1H, d and t, J = 5.6, 16.1 Hz), 6.1 - 6.45 (4H, complex) 5.2 - 5.7 (5H, complex).
Example 149. N-(2-Cinnamylamino)phenvl-S-iso- quinolinesulfonamide 1.8 g of the crystals obtained in Reference Example 43 was dissolved in 18 ml of pyridine, to the solution was added 1.83 g of isoquinolinesulfonyl chloride hydrochloride, and the mixture was stirred for 1 hours at a room temperature. After adding SO ml of water, the reaction mixture was extracted twice with 80 ml of chloroform, and the extract was washed with a saturated sodium chloride aqueous solution, dried over magnesium sulfate and evaporated to remove the solvent under a reduced pressure. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1), to obtain 2.40 g of the title compound as pale reddish crystals.
Melting point: 181 - 1850C; NMR (CDC13) 6 ppm: 3.75 (2H, brd), 4.55 (1H, brs), 6.05 (lH, d and t, J = 5.6, 16.1 Hz), 6.35 (2H, brd), 6.45 (1H, d, J = 16.1 Hz) 6.63 (1H, d, J = 8.3 Hz), 7 7.13 (1K, complex), 7.25 - 7.4 (5H, complex), 7.6 (1H, t, J = 8.2 Hz), 8.15 (1H, d, J = 8.3 Hz), 8.31 (1H, dd, J = 1.0, 8.2 Hz), 8.4 (lH, d, J = 6.6 Hz), 8.65 (lH, d, J = 6.6 Hz), 9.3 (lH, d, J = 1.0 Hz).
Reference Example 44. N- (4-Chlorocinnamyl -1,3- phenylenediamine 3.24 g of metha-phenylenediamine was dissolved in 40 ml of dimethylformamide, to the solution were added 2.07 g of potassium carbonate and 1.87 g of pchlorocinnamyl chloride, and the mixture was subjected to react according to the procedure in Reference Example 40. The resulting residue was applied to a silica gel column and eluted with n-hexane/ethyl acetate (3:1 to 2:1), to obtain 1.70 g of the title compound as a light brown oil.
NMR (CDC13) 6 ppm: 3.65 (2H, brs), 3.90 (2H, dd, J = 1.4, 5.6 Hz), 6.0 - 6.2 (3H, complex), 6.3 (lH, dd, J = 5.6, 15.9 Hz), 6.56 (lH, dd, J = 1.4, 15.9 Hz), 6.97 (lH, t, J = 8.1 Hz), 7.3 (4H, s).
Example 150. N- F - ( 4 - 3- (4-Chlorocinnamylamino ! ) Phenvl 5-isoquinolinesulfonamide 1.7 g of the oil obtained in Reference Example 44 was dissolved in 18 ml of pyridine, to the solution was added 1.99 g of 5-isoquinolinesulfonyl chloride.HCl with stirring under a ice cooling, and the same procedure as described in Example 142 was repeated to obtain 1.45 g of the title compound as a light brown oil.
NMR (CDCl3)S ppm: 3.8 (2H, brd), 3.92 (lH, brs), 6.15 (lH, d and t, J = 5.6, 15.9 Hz), 6.25 (lH, brs), 6.35 (2H, brd), 6.49 (lH, d, J = 15.9 Hz), 6.92 (lH, t, J = 8.1 Hz), 7.3 (4H, s), 7.51 (lH, t, J = 8.3 Hz), 8.1 (lah, d, J = 8.3 Hz), 8.35 (lH, dd, J = 1.0, 8.3 Hz), 8.45 (1H, d, J = 6.1 Hz), 8.65 (lH, d, J = 6.4 Hz), 9.3 (lH, d, J = 1.0 Hz).
Example 151. N- 2- (p-Chlorocinnamylamino phenyl- N-(2-hvdroxyethyl)-5-isoquinolinesulfonamide 1.5 g of the crystals obtained in Example 142 was dissolved in 8 ml of tetrahydrofuran, to the solution were added 1.32 g of triphenylphosphine and 420 mg of ethylene glycol monoacetate, and also added dropwise a solution of 1.01 g of diisopropyl azodicarboxylate in 2 ml of tetrahydrofuran with stirring in a ice bath.
After being removed from the ice bath, the mixture was warmed to a room temperature, stirred for 3 hours, diluted with ethyl acetate and extracted twice with 70 ml of 2 N hydrochloric acid. The aqueous layer was alkalized with sodium bicarbonate and extracted twice with 150 ml of chloroform, and the extract was dried over magnesium sulfate and evaporated under a reduced pressure to remove the solvent. Resulting oily residue was dissolved in 20 ml of methanol and 20 ml of tetrahydrofuran, to the solution was added 20 ml of 1 N sodium hydroxide aqueous solution, and the reaction was carried out at a room temperature for 2 hours. The reaction mixture was diluted with water and extracted twice with 100 ml and 50 ml each of chloroform, and the extract was dried over magnesium chloride and evaporated under a reduced pressure to remove the solvent.The resulting oil was applied to a silica gel column and eluted with chloroform/methanol (100:1 to 50:1), to obtain 1.59 g of the title compound in a yellow amorphous form.
IR (KBr) cm 1 = 1603, 1516, 1491, 1342, 1161, 1139, 835, 758, 604, 509; NMR (CDC13) 6 ppm: 3.09 (lH, m), 3.29 (lH, ddd, J = 13.43, 4.64, 3.18 Hz), 3.47 (lH, m), 3.75 (lH, m), 3.85 (2H, m), 4.33 (lH, ddd, J = 13.43, 8.30, 4.15 Hz), 5.12 (1H, m), 6.16 (lH, at, J = 15.87, 5.62 Hz), 6.23 (lH, dd, J = 8.06, 1.47 Hz), 6.40 (lH, td, J = 7.33, 1.47 Hz), 6.55 (lH, d, J = 16.11 Hz), 6.76 (1H, d, J = 8.54 Hz), 7.15 (1H, t, J = 8.30 Hz), 7.29 (4H, s), 7.63 (lH, t, J = 8.30 Hz), 8.18 (1H, d, J = 8.30 Hz), 8.28 (lH, d, J = 8.30 Hz), 8.28 (lH, d, J = 6.35 Hz), 8.52 (1H, d, J = 6.3 Hz), 9.31 (lH, s).
Example 152. N-82-(p-Chlorocinnamvlamino ! PhenYl E - N- ( 2-dimethylaminoethyl -S-isoauinolinesulfonamide 2.0 g of the crystals obtained in Example 142 was dissolved in 10 ml of tetrahydrofuran, to the solution were added 1.75 g of triphenylphosphine and 520 mg of N,N-dimethyl ethanolamine, and thereto added dropwise a solution of 1.3 g of diisopropyl azodicarboxylate in 3 ml of being tetrahydrofuran with stirring in ice bath.
After being removed from the ice bath, the mixture was warmed to a room temperature, stirred for 3 hours, and then diluted with ethyl acetate, and extracted twice with 100 ml of 2 N hydrochloric acid. The extract was alkalized with a sodium bicarbonate aqueous solution and extracted twice with 200 ml of chloroform. The extract was dried over magnesium sulfate and evaporated under a reduced pressure to remove the solvent, and a resulting oil was applied to a silica gel column and eluted with chloroform/methanol (100:1), to obtain 1.35 g of the title compound in a yellow amorphous form.
IR (KBr) cm : 1603, 1521, 1491, 1458, 1329, 1160, 1137, 834, 749, 601, 507; NMR (CDCl3) 6 ppm: 2.19 (6H, s), 2.15 - 2.55 (2H, m), 3.19 (1H, dt, J = 12.69, 4.15 Hz), 3.56 (2H, m), 4.35 (1H, m), 5.78 (1H, m), 5.89 (1H, dt, J = 15.87, 5.37 Hz), 6.30 - 6.55 (3H, m), 6.64 (1H, dd, J = 7.81, 1.71 Hz), 7.09 (lH, td, J = 7.81, 1.71Hz), 7.23 (2 H, d, J = 9.03 Hz), 7.30 (2H, d, J = 9.03 Hz), 7.57 (1H, dd, J = 8.30, 7.57 Hz), 8.11 (1H, d, J = 8.30 Hz), 8.24 (lH, d, J = 7.57 Hz), 8.40 (lH, d, J = 6.35 Hz), 8.53 (1H, d, J = 6.35 Hz), 9.26 (1H, s).
Examples 153 to 171 In Example 153 to 171 the following general reaction was used.
Example 153. (n = 2, Aryl = 4-chloroDhenoll N- r 2-t4-Chloro-a-methvlcinnamolamino)etholl-5- isoquinolinesulfonamide 7.30 g of N-(2-aminoethyl)-5-isoquinolinesulfonamide was dissolved in 150 ml of methanol, to the solution was added 6.30 g of p-chlorobenzalacetone, and the mixture was stirred at a room temperature for 36 hours. After addition of 1.32 g of sodium tetrahydrideborate with ice-water cooling, the mixture was stirred for 30 minutes. The reaction mixture was concentrated to half of original volume under a reduced pressure, and after adding 300 ml of ethyl acetate, washed three times with water. The aqueous layer was extracted with 100 ml of ethyl acetate, and the extract was washed with water as described above.The ethyl acetate layers were combined, washed twice with a saturated sodium chloride aqueous solution, dried over magnesium sulfate, filtered, and evaporated under a reduced pressure to remove the solvent. The resulting residue was purified using a silica gel column (silica gel: 200 g; eluant: 5% methanol in chloroform), to obtain 6.78 g of the title compound in a colorless amorphous form, while recovering the residual starting material.
1H-NMR (CDC13 , a ppm): 1.06 (3H, d, J = 6.6 Hz), 1.8 - 2.8 (2H, br), 2.57 - 2.64 (2H, m), 2.96 (2H, t, J = 5.7 Hz), 3.06 (1H, dq, J = 7.8, 6.6 Hz), 5.79 (lH, dd, J = 15.8, 7.8 Hz), 6.24 (1H, d, J = 15.8 Hz), 7.19 (2H, dm, J = 8.8 Hz), 7.25 (2H, dm, J = 8.8 Hz), 7.67 (lH, dd, J = 8.0, 7.6 Hz), 8.28 (lH, dt, J = 8.0, 1.0 Hz), 8.42 - 8.46 (2H, m), 8.69 (lH, d, J = 6.1 Hz), 9.35 (lH, d, J = 1.0 Hz).
Example 154. (n = 2, Arvl = phenyl) N- r 2-(a-MethylcinnamYlamino)etholl-5-isoquinoline- sulfonamide Colorless amorphous form; 1H-NHR (CDC13 , s ppm): 2.0 - 3.0 (2H, br), 2.59 2.66 (2H, m), 2.98 (2H, t, J = 5.5 Hz), 3.09 (1H, dq, J = 8.0, 6.6 Hz), 5.80 (1H, dd, J = 15.9, 8.0 Hz), 6.28 (1H, d, J = 15.9 Hz), 7.28 (5H, brs), 7.66 (1H, dd, J = 8.3, 7.3 Hz), 8.17 (1H, brd, J = 8.3 Hz), 8.43 (1H, dd, J = 7.3, 1.2 Hz), 8.44 (lH, d, J = 6.1 Hz), 8.68 (lH, d, J = 6.1 Hz), 9.34 (lH, d, J = 1.0 Hz).
Example 155. (n = 2, Aryl = 2,4-difIuorophenyl) N- r 2-(2 4-Difluoro-t-methvlcinnanvlamino ? ethtll-5- isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.06 (3H, d, J = 6.4 Hz), 1.3 - 2.2 (2H, br), 2.57 - 2.67 (2H, m), 2.96 (2H, t, J = 5.6 Hz), 3.04 (1H, dq, J = 8.0, 6.4 Hz), 5.81 (1H, dd, J = 16.1, 8.0 Hz), 6.35 (1H, d, J = 16.1 Hz), 6.79 (lH, d, J = 8.3 Hz and 1H, ddd, J = 17.6, 8.8, 2.0 Hz), 7.30 (lH, ddd, J = 14.9, 8.3, 2.0 Hz), 7.69 (lH, dd, J = 8.3, 7.3 Hz), 8.29 (lH, dt, J = 8.3, 1.0 Hz), 8.42 8.47 (2H, m), 8.70 (1H, d, J = 6.1 Hz), 9.35 (1H, d, J = 1.0 Hz).
Example 156. (n = 2 Arvl = 2,4-dichlorophenyl) N-[2-(2 4-Dichloro-&alpha;-methylcinnamylamino)ethyl]-5- isopuinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.07 (3H, d, J = 6.6 Hz), 1.5 - 2.5 (2H, br), 2.58 - 2.65 (2H, m), 2.97 (2H, t, J = 5.5 Hz), 3.09 (lH, dq, J = 8.0, 6.6 Hz), 5.75 (1H, dd, J = 15.8, 8.0 Hz), 6.58 (lH, d, J = 15.8 Hz), 7.18 (lH, dd, J = 8.5, 2.0 Hz), 7.28 (1H, d, J = 8.5 Hz), 7.35 (1H, d, J = 2.0 Hz), 7.68 (lH, dd, J = 8.0, 7.3 Hz), 8.18 (1H, td, J = 8.0, 1.0 Hz), 8.42 - 8.47 (2H, m), 8.70 (lH, d, J = 6.1 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 157. (n = 2, Aryl = 3-chlorophenyl) N-[2-(3-Chloro-&alpha;-methylcinnamylamino)ethyl]-5- isoauinolinesulfonamide Colorless amorphous form; H-NMR (CDCl3 , 6 ppm): 1.06 (3H, d, J = 6.6 Hz), 1.3 - 2.4 (2H, br), 2.56 - 2.63 (2H, m), 2.97 (2H, t, J, 5.6 Hz), 3.06 (1H, dq, J = 7.8, 6.6 Hz), 5.80 (1H, dd, J = 15.9, 7.8 Hz), 6.22 (1H, d, J = 15.9 Hz), 7.10 - 7.26 (4H, m), 7.68 (1H, dd, J = 8.1, 7.5 Hz), 8.18 (1H, dt, J = 8.1, 1.0 Hz), 8.42 - 8.47 (2H, m), 8.70 (1H, d, J = 6.1 Hz), 9.35 (lH, d, J = 1.0 Hz).
Example 158. (n = 2, Aryl = 2-nitrophenyl} N-[2-(&alpha;-Methyl-2-nitrocinnamylamino)ethyl]-5-iso- suinolinesulfonamide Light yellow amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.08 (3H, d, J = 6.4 Hz), 1.3 - 2.6 (2H, br), 2.61 - 2.67 (2H, m), 2.99 (2H, t, J = 5.6 Hz), 3.09 (1H, dq, J = 7.8, 6.4 Hz), 5.73 (1H, dd, J = 15.6, 7.8 Hz), 6.73 (1H, d, J = 15.6 Hz), 7.36 7.56 (3H, m), 7.68 (1H, dd, J = 8.3, 7.3 Hz), 7.92 (1H, dd, J = 7.9, 1.2 Hz), 8.42 - 8.47 (2H, m), 8.67 (1H, d, J = 6.1 Hz), 9.31 (1H, d, J = 1.0 Hz).
Example 159. (n = 2, Aryl = 4-nitrophenyl N-F 2- (a-Methyl-4-nitrocinnamylamino ) ethyl 1-S- isoauinolinesulfonylamide Light yellow amorphous form; 1H-NMR (CDC13 , s ppm): 1.10 (3H, d, J = 6.6 Hz), 1.4 - 2.6 (2H, br), 2.60 - 2.67 (2H, m), 2.99 (2H, t, J = 5.5 Hz), 3.14 (1H, dq, J = 7.6, 6.6 Hz), 6.05 (1H, dd, J = 15.9, 7.6 Hz), 6.38 (1H, d, J = 15.9 Hz), 7.40 (2H, dm, J = 8.8 Hz), 7.69 (1H, dd, J = 8.3; 7.5 Hz), 8.14 (2H, dm, J = 8.8 Hz), 8.23 (1H, brd, J = 8.3 Hz), 8.43 8.48 (2H, m), 8.68 (1H, d, J = 6.1 Hz), 9.36 (1H, d, J = 1.0 Hz).
Example 160. (n = 2, Arvl = 4-metholphensl) N- r 2-trz 4-DimetholcinnamYlaminossethvll-5-iso- quinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.05 (3H, d, J = 6.6 Hz), 2.0 - 2.5 (2H, br), 2.33 (3H, s), 2.56 - 2.64 (2H, m), 2.96 (2H, t, J = 5.9 Hz), 3.05 (1H, in), 5.73 (lH, dd, J = 15.9, 7.8 Hz), 6.24 (1H, d, J = 15.9 Hz), 7.09 (2H, brd, J = 8.3 Hz), 7.16 (2H, brd, J = 8.3 Hz), 7.67 (lH, t, J = 8.0 Hz), 8.17 (lH, brd, J = 8.0 Hz), 8.43 (lH, d, J = 8.0 Hz), 8.44 (1H, d, J = 6.1 Hz), 8.68 (1H, a, J = 6.1 Hz), 9.34 (lH, d, J = 1.0 Hz).
Example 161. (n = 2 Arvl = 3,4-methylenedioxy- phenyl) N- F 2- (a-Methyl-3 ,4-methylenedioxycinnamyl- amino ethyl 1 -5-isoauinolinesulfonamide Colorless amorphous form; 1H-NMR (CDC13 , 6 ppm): 1.04 (3H, d, J = 6.3 Hz), 2.56 - 2.63 (2 H, m), 2.95 (2H, t, J = 5.6 Hz), 3.05 (lH, dq, J = 8.0, 6.3 Hz), 5.60 (1H, dd, J = 15.9, 8.0 Hz), 5.95 (2H, s), 6.18 (1H, d, J = 15.9 Hz), 6.70 (lH, dd, J = 7.5, 1.5 Hz), 6.73 (1H, d, J = 7.5 Hz), 6.79 (lah, d, J = 1.5 Hz), 7.68 (1H, dd, J = 8.1, 7.5 Hz), 8.19 (1H, brd, J = 8.1 Hz), 8.42 - 8.46 (2H, m), 8.69 (1H, d, J = 6.1 Hz), 9.35 (1H, d, J = 1.0 Hz).
Example 162. (n = 2, Arvl = 2-pyridyl) N-; 2-F 1-Methyl-3- ( 2-pyridyl -2-propenyl- amino 1 ethyl -5-isopuinolinesulfonamide 1H-NMR (CDC13 , 6 ppm): 1.07 (3H, d, J = 6.6 Hz), 1.5 - 4.0 (2H, br), 2.62 (2H, dt, J = 5.7, 5.7 Hz), 2.97 (2H, t, J = 6.4 Hz), 3.06 (1H, dq, J = 5.6, 6.6 Hz), 6.35 (1H, d, J = 5.6 Hz), 6.37 (1H, s), 7.12 (1H, dddd, J = 7.8, 5.0, 2.0, 1.0 Hz), 7.21 (1H, d, J = 7.8 Hz), 7.62 (lH, td, J = 7.8, 2.0 Hz), 7.68 (IH, dd, J = 8.0, 7.3 Hz), 8.18 (1H, brd, J = 8.0 Hz), 8.44 (lH, d, J = 7.3 Hz), 8.45 (1H, d, J = 7.3 Hz), 8.52 (1H, ddd, J = 5.0, 2.0, 1.0 Hz), 8.67 (1H, d, J = 6.1 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 163. (n = 2, Aryl = 4-pyridvi) N-82- r 1-MethYl-3-(4-psridyl)-2-roPenolaminoleth- vl)-5-isosuinolinesuIfonvlamide Colorless amorphous form; H-NMR (CDCl3 , s ppm): 1.09 (3H, d, J = 6.3 Hz), 1.2 - 1.9 (2H, br), 2.59 - 2.65 (2H, m), 2.98 (2H, t, J = 6.0 Hz), 3.12 (1H, dq, J = 7.3, 6.3 Hz), 6.06 (lH, dd, J = 15.9, 7.3 Hz), 6.26 (1H, d, J = 15.9 Hz), 7.14 (2H, dd, J = 6.1, 1.5 Hz), 7.69 (1H, dd, J = 8.1, 7.5 Hz), 8.19 (lH, brd, J = 8.1 Hz), 8.42 - 8.47 (2H, m), 8.51 (2H, dd, J = 6.1, 1.5 Hz), 8.68 (1H, d, J = 6.3 Hz), 9.35 (1H, d, J = 1.0 Hz).
Example 164. (n = 2, Arvl = 2-thienyl) N-X2- r 1-MethYl-3-(2-thienol)-2-roPenvlaminol ethvl} -5-isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDC13 , # ppm): 1,05 (3H, d, J = 6.6 Hz), 1.2 - 2.5 (2H, br), 2.56 - 2.64 (2H, m), 2.93 - 3.05 (3H, m), 5.65 (1H, dd, J = 15.6, 8.0 Hz), 6.41 (1H, d, J = 15.6 Hz), 6.85 (1H, dd, J = 3.7, 2.4 Hz), 6.94 (lH, dd, J = 4.9, 3.7 Hz), 7.13 (1H, dd, J = 4.9, 2.4 Hz), 7.68 (1H, dd, J = 8.3, 7.5 Hz), 8.19 (1H, brd, J = 8.3 Hz), 8.42 - 8.46 (2H, m), 8.69 (1H, d, J = 6.1 Hz), 9.35 (1H, d, J = 1.0 Hz).
Example 165. (n = 2, Aryl = 2-furyl) N-{2-[3-(2-Furyl)-1-methyl-2-propenylamino]ethyl -5-isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , s ppm): 1.04 (3H, d, J = 6.4 Hz), 1.3 - 1.5 (2H, br), 2.59 (2H, td, J = 6.0, 4.9 Hz), 2.95 (2H, t, J = 6.0 Hz), 2.98 (1H, dq, J = 7.8, 6.4 Hz), 5.75 (1H, dd, J = 15.9, 7.8 Hz), 6.10 (1H, d, J = 15.9 Hz), 6.16 (1H, d, J = 3.2 Hz), 6.35 (1H, dd, J = 3.2, 1.9 Hz), 7.32 (1H, d, J = 1.9 Hz), 7.68 (1H, dd, J = 8.3, 7.5 Hz), 8.19 (1H, brd, J = 8.3 Hz), 8.42 - 8.47 (2H, m), 8.69 (1H, d, J = 6.1 Hz), 9.35 (1H, d, J = 1.0 Hz).
Example 166. (n = 2, Arvl = 4-fluorophenyl) N-[2-(4-Fluoro-&alpha;-methylcinnamylamino)ethyl]-5- isopuinolinesulfonamide Colorless amorphous form; 1H-NMR (CDC13 , 6 ppm): 1.06 (3H, d, J = 6.4 Hz), 1.3 - 2.0 (2H, br), 2.57 - 2.63 (2H, m), 2.95 (2H, t, J = 5.5 Hz), 3.05 (1H, dq, J = 8.0, 6.4 Hz), 5.72 (1H, dd, J = 15.9, 8.0 Hz), 6.25 (lH, d, J = 15.9 Hz), 6.98 (2H, tm, J = 8.7 Hz), 7.20 - 7.27 (2H, m), 7.68 (1H, dd, J = 8.1, 7.3 Hz), 8.18 (lH, brd, J = 8.1 Hz), 8.42 - 8.47 (2H, m), 8.69 (lH, d, J = 6.1 Hz), 9.35 (lH, d, J = 1.0 Hz).
Example 167. (n = 2, Aryl = 4-bromophenyl) N- [2-(4-Bromo-&alpha;-methylcinnamolamino)ethyl]-5- isocuinolinesulfonamide Colorless amorphous form; H-NMR (CDCl3 , 6 ppm): 106 (3H, d, J = 6.4 Hz), 1.3 - 2.2 (2H, br), 2.56 - 2.63 (2H, m), 2.95 (2H, t, J = 5.7 Hz), 3.05 (1H, dq, J = 8.0, 6.4 Hz), 5.79 (1H, dd, J = 15.9, 8.0 Hz), 6.22 (1H, d, J = 15.9 Hz), 7.13 (2H, dm, J = 8.5 Hz), 7.41 (2H, dm, J = 8.5 Hz), 7.68 (1H, dd, J = 8.3, 7.4 Hz), 8.19 (1H, brd, J = 8.3 Hz), 8.42 8.46 (2H, m), 8.69 (1H, d, J = 6.1 Hz), 9.35 (1H, d, J = 1.0 Hz).
Example 168. (n = 2, Aryl = 4-isopropylphenyl) N-[2-(4-isopropyl-&alpha;-methylcinnamolamino)ethyl]-5- isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.05 (3H, d, J = 6.6 Hz), 1.24 (6H, d, J = 6.8 Hz), 1.5 - 2.5 (2H, br), 2.56 2.63 (2H, m), 2.80 - 3.05 (3H, m), 5.74 (lH, ad,. J = 15.9, 8.0 Hz), 6.24 (lH, d, J = 15.9 Hz), 7.16 (2H, d, J = 8.6 Hz), 7.20 (2H, d, J = 8.6 Hz), 7;66 (lH, dd, J = 8.3, 7.3 Hz), 8.17 (1H, brd, J = 8.3 Hz), 8.43 (lH, dd, J = 7.3, 1.0 Hz), 8.44 (1H, d, J = 6.1 Hz), 8.69 (1H, d, J = 6.1 Hz), 9.34 (lH, d, J = 1.0 Hz).
Example 169. (n = 2, Aryl = 4-methoxyphenyl) N-[2-(4-Methoxy-&alpha;-methylcinnamylamino)ethyl]-5- isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.05 (3H, d, J = 6.4 Hz), 1.5 - 2.5 (2H, br), 2.56 - 2.63 (2H, m), 2.96 (2H, t, J = 5.6 Hz), 3.02 (1H, dq, J = 8.0, 6.4 Hz), 3.81 (3H, s), 5.64 (lH, dd, J = 15.9, 8.0 Hz), 6.21 (lH, d, J = 15.9 Hz), 6.83 (2H, dm, J = 8.8 Hz), 7.20 (2H, dm, J = 8.8 Hz), 7.67 (1H, dd, J = 8.3, 7.3 Hz), 8.19 (lH, brd, J = 8.3 Hz), 8.44 (1H, dd, J = 7.3, 1.2 Hz), 8.44 (1H, d, J = 6.1 Hz), 8.69 (1H, d, J = 6.1 Hz), 9.34 (lH, d, J = 1.0 Hz).
Example 170. (n = 2, Aryl = 4-hydroxyphenyl) N- r 2- (4-hydroxy-a-methylcinnamylamino ) ethyl 1-5-iso- quinolinesulfonamide Colorless crystals; Melting point: 70 - 730C; 1H-NMR (CDCl3 , 6 ppm): 1.06 (3H, d, J = 6.4 Hz), 2.61 (2H, brt, J = 5.7 Hz), 3.00 (2H, brt, J = 5.7 Hz), 3.05 (lH, dq, J = 8.0, 6.4 Hz), 3.3 - 3.5 (3H, br), 5.61 (1H, dd, J = 15.9, 8.0 Hz), 6.19 (1H, d, J = 15.9 Hz), 6.75 (2H, brd, J = 8.5 Hz), 7.10 (2H, brd, J = 8.5 Hz), 7.65 (1H, dt, J = 8.3, 7.3 Hz), 8.16 (1H, brd, J = 8.3 Hz), 8.40 - 8.46 (2H, m), 8.59 (1H, d, J = 6.1 Hz), 9.32 (1H, d, J = 1.0 Hz).
Example 171. (n = 3, Aryl = Phenol) N-[3-&alpha;-Methylcinnamylamino)propyl]-5-isoquinoline sulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.27 (3H, d, J = 6.6 Hz), 1.50 - 1.60 (2H, m), 1.6 - 2.5 (2H, br), 2.60 - 2.67 (2H, m), 3.01 - 3.09 (2H, m), 3.24 (1H, dq, J = 7.8, 6.6 Hz), 5.91 (1H, dd, J = 15.9, 7.8 Hz), 6.40 (lH, d, J = 15.9 Hz), 7.30 (5H, m), 7.68 (1H, dd, J = 8.0, 7.3 Hz), 8.18 (1H, brd, J = 8.0 Hz), 8.43 (1H, dd, J = 7.3, 1.2 Hz), 8.47 (1H, d, J = 6.1 Hz), 8.67 (1H, d, J = 6.1 Hz), 9.36 (1H, d, J = 1.0 Hz).
Examples 172 to 188.
In Examples 172 to 188, the following general reaction was used.
Example 172. (n = 2, m = 1, Aryl = 4-chlorophenyl) N-[2-(4-Chlorocinnamylamino)ethyl]-5-isoquinoline- sulfonamide 2.01 g of N-(2-aminoethyl)-5-isoquinolinesulfonamide was dissolved in 30 ml of methanol, to the solution was added 1.60 g of p-chlorocinnamaldehyde, and the mixture was stirred for one hour at a room temperature. After an addition.of 350 mg of sodium tetrahydrideborate in portions with ice cooling, the mixture was stirred for 30 minutes. After an addition of ethyl acetate, the reaction mixture was sequentially washed three times with water, and then twice with a saturated sodium chloride aqueous solution, and dried over magnesium sulfate. The mixture was filtered and evaporated to remove the solvent under a reduced pressure.A residue was purified using a silica gel column (silica gel 80 g, eluant: 5% methanol in chloroform), and resulting crystals were washed with benzene/hexane (1:1), to obtain 2.30 g of the title compound as colorless crystals.
Melting point: 120 - 1230C; 1H-NMR (CDCl3 , 6 ppm): 1.8 - 3.5 (2H, br)., 2.64 2.70 (2H, m), 2.97 - 3.03 (2H, m), 3.14 (2H, dd, J = 6.1, 1.2 Hz), 6.00 (lH, dt, J = 15.9, 6.1 Hz), 6.32 (lH, d, J = 15.9 Hz), 7.21 (2H,dd, J = 8.8, 2.4 Hz), 7.28 (2H, dd, J = 8.8, 2.4 Hz), 7.69 (lH, dd, J = 8.3, 7.4 Hz), 8.19 (lH, dd, J = 8.3, 1.0 Hz), 8.42 - 8.47 (2H, m), 8.69 (1H, d, J = 6.1 Hz), 9.34 (lH, d, J = 1.0 Hz).
Example 173. In = 2, m = 1, Aryl = phenyl) N- ( 2-Cinnamylaminoethyl ) 5-isoquinolinesulfonamide Colorless amorphous form; H-NMR (CDC13 , 6 ppm): 1.8 - 2.8 (2H, br), 2.64 2.69 (2H, m), 2.97 - 3.03 (2H, m), 3.14 (lH, dd, J = 6.3, 1.2 Hz), 6.02 (1H, dt, J = 15.9, 6.3 Hz), 6.46 (1H, dt, J = 15.9, 1.2 Hz), 7.30 (5H, s), 7.68 (lH, dd, J = 8.1, 7.3 Hz), 8.18 (1H, dt, J = 8.1, 1.0 Hz), 8.42 8.48 (2H, m), 8.70 (lH, d, J = 6.1 Hz), 9.34 (lH, d, J = 1.0 Hz).
Example 174. (n = 2, m = 1, Arvl = 4-dimethyl aminophenyl) N-[2-(4-Dimetolaminocinnamylamino)ethyl]-5-iso- quinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 2.65 (2H, brs), 2.70 (2H, dd, J = 6.1, 4,9 Hz), 2.96 (6H, s), 3.02 (2H, dd, J = 6.1, 4.9 Hz), 3.14 (2H, dd, J = 6.6, 1.0 Hz), 5.81 (lH, dt, J = 15.9, 6.5 Hz), 6.27 (1H, brd, J = 15.9 Hz), 6.66 (2H, brd, J = 8.8 Hz), 7.20 (2H, brd, J = 8.8 Hz), 7.68 (1H, dd, J = 8.0, 7.5 Hz), 8.18 (lH, dt, J = 8.0, 1.0 Hz), 8.44 (1H, d, J = 6.0 Hz and lH, d, J = 7.5 Hz), 8.71 (1H, d, J = 6.1 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 175. (n = 2, m = 1, Aryl = 4-fluorophenyl) N-[2-4-Fluorocinnamylamino)ethyl]-5-isoquinoline- sulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.5 - 2.5 (2H, br), 2.63 2.69 (2H, m), 2.97 - 3.02 (2H, m), 3.12 (2H, dd, J = 6.1, 1.2 Hz), 5.94 (1H, dt, J = 15.9, 6.1 Hz), 6.33 (lH, d, J = 15.9 Hz), 7.00 (2H, ddd, J = 8.6, 8.6, 2.2 Hz), 7.27 (2H, ddd, J = 8.6, 5.3, 2.2 Hz), 7.69 (lH, dd, J = 8.2, 7.6 Hz), 8.19 (1H, brd, J = 8.2 Hz), 8.42 - 8.48 (2H, m), 8.70 (1H, d, J = 6.1 Hz), 9.35 (lH, d, J = 1.0 Hz).
Example 176. (n = 2, m = 1, Aryl = 4-bromophenyl) N-[2-(4-Bromophenylcinnamylamino)ethyl]-5-iso- cruinolinesulfonamide Colorless crystals; Melting point: 124 - 1270C; 1H-NMR (CDCl3 , 6 ppm): 2.0 - 3.5 (2H, br), 2.64 2.69 (2H, m), 2.97 - 3.03 (2H, m), 3.13 (2H, dd, J = 6.1, 1.0 Hz), 6.01 (1H, dt, J = 15.9, 6.3 Hz), 6.30 (lH, d, J = 15.9 Hz), 7.14 (2H, dm, J = 8.6 Hz), 7.41 (2H, dm, J = 8.6 Hz), 7.68 (1H, dd, J = 8.3, 7.5 Hz), 8.18 (lH, brd, J = 8.3 Hz), 8.43 - 8.47 (2H, m), 8.68 (lH, d, J = 6.1 Hz), 9.34 (lH, d, J = 1.0 Hz).
Example 177. (n = 2, m = 1 Aryl = 4-isopropyl- phenyl) N-[2-(4-Isopropylcinnamylamino)ethyl]-5-iso- quinolinesulfonamide Colorless amorphous form; 1H-NMR (CDC13 , 6 ppm): 1c24 (6H, d, J = 7.1 Hz), 2.0 - 2.3 (2H, br), 2.63 - 2.69 (1H, m), 2.87 (1H, q, J = 7.1 Hz), 2.97 - 3.03 (2H, m), 3.13 (1H, dd, J = 6.3, 1.2 Hz), 5.97 (lH, dt, J = 15.9, 6.3 Hz), 6.33 (lH, brd, J = 15.9 Hz), 7.16 (2H, dm, J = 8.3 Hz), 7.23 (2H, dm, J = 8.3 Hz), 7.67 (lH, dd, J = 8.3, 7.3 Hz), 8.17 (lH, d, J = 8.3 Hz), 8.43 - 8.47 (2H, m), 8.69 (lH, d, J = 6.1 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 178. (n = 2, m = 1, Aryl = 4-methoxy phenyl) N-[2-(4-Methoxycinnamylamino)ethyl]-5-isoquinoline- sulfonamide Colorless crystals; Melting point: 92 - 950C; NMR (CDCl3 , 6 ppm): 2.0 - 3.0 (2H, br), 2.63 2.69 (2H, m), 2.97 - 3.06 (2H, m), 3.11 (2H, dd, J = 6.3, 1.2 Hz), 3.81 (3H, s), 5.88 (1H, dt, J = 15.9, 6.3 Hz), 6.30 (1H, d, J = 15.9 Hz), 6.84 (2H, dm, J = 8.8 Hz), 7.23 (2H, dm, J = 8.8 Hz), 7.68 (1H, dd, J = 8.3, 7.5 Hz), 8.19 (1H, brd, J = 8.3 Hz), 8.42 - 8.47 (2H, m), 8.69 (lH, d, J = 6.1 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 179. (n = 2, m = 1, Arvl = 4-trifluoro methylphenyl) N-[2-(4-Trifluorometholcinnamylamino)ethyl]-5- isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.5 - 2.5 (2H, br), 2.66 2.72 (2H, m), 2.98 - 3.04 (2H, m), 3.19 (2H, dd, J = 6.1, 1.2 Hz), 6.14 (1H, dt, J = 15.9, 6.1 Hz), 6.41 (1H, d, J = 15.9 Hz), 7.39 (2H, brd, J = 8.3 Hz), 7.56 (2H, brd, J = 8.3 Hz), 7.69 (lH, dd, J = 8.3, 7.3 Hz), 8.20 (1H, brd, J = 8.3 Hz), 8.42 - 8.48 (2H, m), 8.70 (1H, d, J = 6.1 Hz), 9.35 (lH, d, J = 1.0 Hz).
Example 180. (n 3 2, m = 2, Aryl = 4-trifluoro methylphenyl) N-{2- [5-(4-Trifluoromethylphenyl)-2,4-pentadien amino 1 ethvl -5-isoouinolinesulfonamide Light yellow amorphous form; 1H-NMR (CDC13 , 6 ppm): 1.0 - 2.5 (2H, br), 2.61 2.67 (2H, m), 2.96 - 3.05 (2H, m), 3.08 (2H, dd, J = 6.3, 1.0 Hz), 5.69 (lH, dt, J = 15.0, 6.3 Hz), 6.19 (1H, dd, J = 15.0, 10.0 Hz), 6.48 (1H, d, J = 15.7 Hz), 6.75 (1H, dd, J = 15.7, 10.0 Hz), 7.47 (2H, brd, J = 8.3 Hz), 7.57 (2H, brd, J = 8.3 Hz), 7.72 (lH, dd, J = 8.3, 7.3 Hz), 8.20 (1H, dt, J = 8.3, 1.0 Hz), 8.43 - 8.48 (2H, m), 8.72 (1H, d, J = 6.1 Hz), 9.36 (lH, d, J = 1.0 Hz).
ExamPle 181. (n = 2, m = 3 Arvl - 4-trifluoro methylphenyl) N-; 2-F 7- ( 4-Trifluoromethylphenyl ) -2,4, 6 -heptatri - enylaminol etholT-5-isoquinolinesulfonamide Light yellow amorphous form; 1H-NMR (CDC13 , 6 ppm): 2.0 - 3.5 (2H, br), 2.61 2.67 (2H, m), 2.95 - 3.01 (2H, m), 3.07 (2H, dd, J = 6.3, 1.0 Hz), 5.59 (1H, dt, J = 14.6, 6.3 Hz), 6.05 6.22 (1H, m), 6.29 - 6.34 (2H, m), 6.55 (1H, d, J = 15.6 Hz), 6.81 - 6.93 (1H, m), 7.47 (2H, brd, J = 8.3 Hz), 7.55 (2H, brd, J = 8.3 Hz), 7.71 (1H, dd, J = 8.3, 7.3 Hz), 8.21 (1H, brd, J = 8.3 Hz), 8.43 - 8.48 (2H, m), 8.72 (1H, d, J = 6.1 Hz), 9.37 (1H, d, J = 1.0 Hz).
Example 182. Fn = 2, m = 1, Arvl = 4-(2-methoxy ethoxyMmethoxoshenoll N-X2- r 4-(2-methoxsethoxo)methoxvcinnamolaminol ethyl}-5-isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.8 - 2.7 (2H, br), 2.63 2.69 (2H, m), 2.96 - 3.02 (2H, m), 3.11 (2H, dd, J = 6.4, 1.2 Hz), 3.37 (3H, s), 3.53 - 3.58 (2H, m), 3.80 3.85 (2H, m), 5.27 (2H, s), 5.90 (1H, dt, J = 15.9, 6.4 Hz), 6.30 (1H, d, J = 15.9 Hz), 6.99 (2H, dm, J = 8.8 Hz), 7.23 (2H, dm, J = 8.8 Hz), 7.68 (1H, dd, J = 8.3, 7.3 Hz), 8.19 (lH, dt, J = 8.3, 1.0 Hz), 8.42 8.47 (2H, m), 8.70 (lH, d, J = 6.1 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 183. (n = 2, m = 1 Aryl = 4-hydroxy- phenyl) N-[2-(4-Hydroxycinnamylamino)ethyl]-5-isoquinoline- sulfonamide Colorless crystals; Melting point: 156 - 1590C; 1H-NMR (DMSO-d6 , s ppm): 2.44 (2H, brt, J = 6.3 Hz), 2.88 (2H, brt, J = 6.3 Hz), 3.01 (2H, brd, J = 6.1 Hz), 3.39 (3H, br), 5.83 (1H, dt, J = 15.9, 6.1 Hz), 6.20 (1H, d, J = 15.9 Hz), 6.70 (2H, brd, J = 8.3 Hz), 7.14 (2H, brd, J = 8.3 Hz), 7.81 (1H, t, J = 7.8 Hz), 8.34 - 8.46 (3H, m), 8.68 (1H, d, J = 6.1 Hz), 9.46 (1H, d, J = 1.0 Hz).
Example 184. (n = 2, m = 1 Aryl = 1-naphthyl) N-{2-[3-(1-Naphthyl)-2-propenylamino]ethyl}-5-iso- quinolinesulfonamide Colorless crystals; Melting point: 135 - 1380C; 1H-NMR (CDCl3 , s ppm): 1.5 - 4.0 (2H, br), 2.68 2.73 (2H, m), 3.01 - 3.06 (2H, m), 3.26 (1H, dd, J = 6.3, 1.5 Hz), 6.00 (1H, dt, J = 15.6, 6.3 Hz), 7.10 (1H, d, J = 15.6 Hz), 7.43 - 7.51 (4H, m), 7.61 (1H, dt, J = 8.3, 7.3 Hz), 7.78 (1H, dd, J = 7.1, 2.7 Hz), 7.83 7.89 (1H, m), 7.97 - 8.02 (1H, m), 8.07 (1H, brd, J = 8.3 Hz), 8.44 (1H, dd, J = 7.3, 1.0 Hz), 8.44 (1H, d, J = 6.1 Hz), 8.68 (1H, d, J = 6.1 Hz), 9.27 (1H, d, J = 1.0 Hz).
Example 185. (n = 2, m = 1, Arvl = 3,4,5-tri methoxyphenyl N-[2-(3,4,5-Trimethoxycinnamylamino)ethyl]-5- isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , # ppm): 1.5 - 2.6 (2H, br), 2.65 2.71 (2H, m), 2.97 - 3.03 (2H, m), 3.15 (2H, dd, J = 6.3, 1.2 Hz), 3.85 (3H, s), 3.88 (6H, s), 5.97 (lH, dt, J = 15.9, 6.3 Hz), 6.31 (1H; d, J = 15.9 Hz), 6.55 (2H, s), 7.69 (lH, dd, J = 8.3, 7.5 Hz), 8.20 (1H, brd, J = 8.3 Hz), 8.43 (lH, brd, J = 6.1 Hz), 8.46 (lH, dd, J = 7.5, 1.2 Hz), 8.70 (lH, d, J = 6.1 Hz), 9.35 (lH, d, J = 1.0 Hz).
Example 186. (n = 2 m = 1, Aryl = 4-methoxv carbonylphenyl) N-[2-(4-Carbomethoxycinnamylamino)ethyl]-5-iso- quinolinesulfonamide Colorless crystals; Melting point 110 - 1130C H-NMR (CDCl3 , 6 ppm): 1.2 - 2.0 (2H, br), 2.65 2.70 (2H, m), 2.97 - 3.02 (2H, m), 3.17 (2H, dd, J = 5.9, 1.2 Hz), 3.92 (3H, s), 6.15 (lH, dt, J = 15.9, 5.9 Hz), 6.41 (1H, d, J = 15.9 Hz), 7.36 (2H, dm, J = 8.3 Hz), 7.69 (1H, dd, J = 8.3, 7.3 Hz), 7.98 (2H, dm, J = 8.3 Hz), 8.19 (lH, brd, J = 8.3 Hz),8.43 (1H, brd, J = 6.1 Hz), 8.46 (1H, dd, J = 7.3, 1.5 Hz), 8.71 (1H, d, J = 6.1 Hz), 9.35 (lH, d, J = 1.0 Hz).
Example 187. (n = 2, m = 1, Arvl = 4-carboxv- phenyl) N-F 2- (4-Carboxycinnamylamino)ethyl]-5-isoquinoline- sulfonamide Colorless crystals; Melting points: 239 to 2400C (decomposed); 1H-NMR (DMSO-d6 , 6 ppm): 2.49 (2H, brt, J = 6.3 Hz), 2.91 (2H, brt, J = 6.3 Hz), 3.13 (2H, brd, J = 5.7 Hz), 3.0 - 4.0 (3H, br), 6.24 (1H, dt, J = 16.1, 5.7 Hz), 6.44 (1H, d, J = 16.1 Hz), 7.44 (2H, brd, J = 8.3 Hz), 7.82 (1H, dd, J = 8.3, 7.3 Hz), 7.88 (2H, brd, J = 8.3 Hz), 8.36 (1H, dd, J = 7.3, 1.2 Hz), 8.42 (1H, brd, J = 8.3 Hz), 8.44 (1H, brd, J = 6.1 Hz), 8.69 (1H, d, J = 6.1 Hz), 9.46 (1H, d, J = 1.0 Hz) ExamPle 188. (n = 3 m = 1, Aryl = phenyl) N-(3-Cinnamolaminopropy)-5-isoquinolinesulfonamide Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.5 - 2.2 (2H, br), 1.59 (2H, tt, J = 5.6, 5.6 Hz), 2.66(2H, t, J = 5.6 Hz), 3.06 (2H, t, J = 5.6 Hz), 3.30 (2H, dd, J = 6.1, 1.5 Hz), 6.21 (lH, dt, J = 15.9, 6.1 Hz), 6.52 (1H, d, J = 15.9 Hz), 7.21 - 7.40 (5H, m), 7.67 (1H, dd, J = 8.3, 7.5 Hz), 8.18 (1H, d, J = 8.3 Hz), 8.43 (1H, dd, J = 7.5, 1.2 Hz), 8.44 (1H, d, J = 6.1 Hz), 8.63 (1H, d, J = 6.1 Hz), 9.35 (1H, d, J = 1.0 Hz).
Example 189. N-{2- [3-(4-Chlorophenol)-2-propynyl- amino]ethyl}-5-isopuinolinesulfonamide (compound 189-I; N-{2-Bis-(3-( (4-chlorophenyl) )-2-propynyl) amino]ethyl}-5-isoquinolinesulfornamide (compound 189-II); N-[3-(4-Chlorophenyl)-2-propynyl]-N-[ 2-(p-chloroph enY)-2-PropynslaminolethYl1-5-isoauinolinesulfonam ide (compound 189-111)
(Compound 189-III) 1.90 g of N-(2-aminoethyl)-5-isoquinolinesulfonamide and 1.39 g of 3-p-chlorophenyl-2-propynyl chloride were dissolved in 10 ml of dimethylformamide, to the solution was added 1.38 g of potassium carbonate, and the mixture was stirred for 24 hours at a room temperature.The reaction mixture was poured to 100 ml of ethyl acetate, washed sequentially three times with water and then twice with a saturated sodium chloride aqueous solution, dried over magnesium sulfate, filtered, and evaporated to remove the solvent under a reduced pressure. The resulting residue was separated and purified using a silica gel column (silica gel 100 g; eluant: 5% methanol/chloroform). By crystallizing from a mixture of ether-hexane, 855 mg of compound 189-I as colorless crystals 220 mg of compound 189-II, and 214 mg of compound 189-III in colorless amorphous form were obtained.
Compound 189-I Colorless crystals; Melting point: 120 - 1230C; H-NMR (CDC13 , 6 ppm): 1.30 - 1.80 (2H, br), 2.74 - 2.80 (2H, m), 3.00 - 3.05 (2H, m), 3.39 (1H, s), 7.26 (4H, s), 7.69 (1H, dd, J = 8.3, 7.3 Hz), 8.20 (1H, dt, J = 8.3, 1.0 Hz), 8.42 (1H, dt, J = 6.1, 1.0 Hz), 8.46 (lH, dd, J = 7.3, 1.2 Hz), 8.70 (1H, d, J = 6.1 Hz), 9.36 (1H, d, J = 1.0 Hz).
ComPound 189-Il Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 2.69 - 2.74 (2H, m), 3.01 - 3.09 (2H, m), 3.43 (4H, s), 5.48 (lH, t, J = 5.0 Hz), 7.27 (4H, dm, J = 9.0 Hz), 7.30 (4H, dm, J = 9.0 Hz), 7.68 (1H, dd, J = 8.3, 7.3 Hz), 8.20 (lH, brd, J = 8.3 Hz), 8.42 (1H, brd, J = 6.1 Hz), 8.47 (1H, dd, J = 7.3, 1.2 Hz), 8.66 (1H, d, J = 6.1 Hz), 9.35 (1H, d, J = 1.0 Hz).
Compound 189-Ill Colorless amorphous form; 1H-NMR (CDCl3 , 6 ppm): 1.58 (1H, br), 3.02 (2H, t, J = 6.0 Hz), 3.55 (2H, t, J = 6.0 Hz), 3.64 (2H, s), 4.50 (2H, s), 6.81 (2H, dm, J = 8.8 Hz), 7.15 (2H, dm, J = 8.8 Hz), 7.27 (2H, dm, J = 9.0 Hz), 7.31 (2H, dm, J = 9.0 Hz), 7.66 (1H, dd, J = 8.3, 7.3 Hz), 8.13 (lH, brd, J = 8.3 Hz), 8.48 (1H, dd, J = 7.3, 1.2 Hz), 8.57 (1H, brd, J = 6.1 Hz), 8.69 (1H, d, J = 6.1 Hz), 9.26 (1H, d, J = 1.0 Hz).
Example 190. N-[2-(4-Chloro-N-methylcinnamvl- amino ) ethyl 1 -5-isoauinolinesulfonamide
1.50 g of the product of Example 172 was dissolved in 10 ml of chloroform, to the solution was added 3 ml of methyl iodide at a room temperature, and the mixture was stirred for 40 minutes. Excess methyl iodide was immediately evaporated off under a reduced pressure, and resulting residue was purified on a silica gel column (silica gel 50 g, eluant: 5% methanol in chloroform), to obtain 720 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , s ppm): 1.4 - 2.1 (1H, br), 1.95 (3H, s), 2.37 (2H, t, J = 5.5 Hz), 2.92 - 3.00 (3H, m), 5.97 (1H, at, J = 15.9, 6.6 Hz), 6.34 (1H, d, J = 15.9 Hz), 7.25 (2H, dm, J = 8.8 Hz), 7.28 (2H, dm, J = 8.8 Hz), 7.68 (lH, dd, J = 8.3, 7.8 Hz), 8.19 (1H, brd, J = 8.3 Hz), 8.44 (1H, brd, J = 6.1 Hz), 8.45 (1H, dd, J = 7.8, 1.5 Hz), 8.69 (1H, d, J = 6.1 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 191. 1- (4-Chlorocinnamyl )-4- (5-iso- quinolinesulfonyl)piperazine
1.31 g of the product of Example 172 was dissolved in 3 ml of dimethylformamide, to the solution were added 644 mg of 1,2-dibroinoethane and 1.13 g of anhydrous potassium carbonate at a room temperature, and the mixture was stirred for 24 hours. After adding 100 ml of ethyl acetate, the ethyl acetate layer was sequentially washed with water and a saturated sodium chloride aqueous solution twice in each case, and dried over magnesium sulfate. The solution was filtered and evaporated under a reduced pressure, and resulting residue was purified on a silica gel column (silica gel 50 g, eluant: 5% methanol in chloroform), to obtain 356 mg of the title compound in a colorless amorphous form, while recovering the residual starting material.
1H-NMR (CDC13 , 6 ppm): 2.53 (4H, brt, J = 4.9 Hz), 3.10 (2H, dd, J = 6.6, 1.2 Hz), 3.20 (4H, brt, J = 4.9 Hz), 6.07 (lH, dt, J = 15.9, 6.6 Hz), 6.43 (lH, d, J = 15.9 Hz), 7.24 (4H, s), 7.72 (1H, dd, J = 8.1, 7.3 Hz), 8.22 (lH, brd, J = 8.1 Hz), 8.37 (1H, dd, J = 7.3, 1.2 Hz), 8.55 (lH, brd, J = 6.1 Hz), 8.68 (1H, d, J = 6.1 Hz), 9.34 (1H, d, J = 1.0 Hz).
Example 192. N-Ethyl-N-[2-(4-chloro-N-ethyl- cinnamylamino)ethyl]-5-isoquinolinesulfonamide
The procedure as described in Example 191 was repeated except that 1.31 g of the product of Example 172 and 2.14 g of ethyl iodide as N-alkylating agent were used, to obtain 720 mg of the title compound in a colorless amorphous form.
1H-NMR (CDC13 , 6 ppm): 0.99 (3H, t, J = 7.1 Hz), 1.05 (3H, t, J = 7.1 Hz), 2.53 (2H, q, J = 7.1 Hz), 2.61 (2H, t, J = 7.8 Hz), 3.19 (2H, dd, J = 6.5, 1.2 Hz), 3.33 - 3.43 (4H, m), 6.12 (lH, dt, J ='15.9, 6.5 Hz), 6.32 (1H, d, J = 15.9 Hz), 7.26 (4H, s), 7.62 (lH, dd, J = 8.1, 7.3 Hz), 8.14 (lH, dd, J = 8.1 Hz), 8.35 (lH, dd, J = 7.3, 1.2 Hz), 8.42 (lH, brd, J = 6.1 Hz), 8.66 (lH, d, J = 6.1 Hz), 9.31 (1H, d, J = 1.0 Hz).
Example 193. N-F2-(4-Chloro-N-formylcinnamyl- amino]ethyl]-5-isoquinolinesulfonamide
3 ml of formic acid and 3 ml of acetic anhydride were mixed and stirred at a room temperature, and to the mixture was added 1.41 g of the product of Example 172, and the mixture was stirred for one hour. The reaction mixture was added to 50 ml of ethyl acetate and 30 ml of saturated sodium carbonate aqueous solution with ice, and the mixture was stirred, and after foaming was terminated, the ethyl acetate layer was sequentially washed twice with water and once with a saturated sodium chloride aqueous solution, and dried over magnesium sulfate, filtered and evaporated under a reduced pressure.The resulting residue was purified on a silica gel column (silica gel 60 g, eluant: 2% methanol in chloroform), to obtain 1.49 g of the title compound as a mixture of twe isomers (3:2) in a colorless amorphous form.
1H-NMR (CDC13 , E ppm): 3.07 - 3.16 (2H, m), 3.39 - 3.47 (2H, m), 3.90 (0.6 x 2H, dd, J = 6.3, 1.0 Hz), 4.03 (0.4 x 2H, dd, J = 6.3, 1.0 Hz), 5.93 (0.6H, dt, J = 15.9, 6.3 Hz), 6.01 (0.4H, dt, J = 15.9, 6.3 Hz), 6.40 (0.4H, d, J = 15.9 Hz), 6.44 (0.6H, d, J = 15.9 Hz), 7.20 (0.6 x 2H, d, J =8.8 Hz), 7.21 (0.4 x 2H, d, J = 8.8 Hz), 7.26 (0.6 x 2H, d, J = 8.8 Hz), 7.27 (0.4 x 2H, d, J = 8.8 Hz), 7.61 (0.6H, dd, J = 8.0, 7.6 Hz), 7.64 (0.4H, dd, J = 8.0, 7.6 Hz), 8.05 (0.6H, s), 8.09 (0.4H, s), 8.16 (0.6H, brd, J = 8.0 Hz), 8.17 (0.4H, brd, J = 8.0 Hz), 8.33 - 8.42 (2H, m), 8.60 (0.4H, d, J = 6.1 Hz), 8.65 (0.6H, d, J = 6.1 Hz), 9.33 (1H, d, J = 1.0 Hz).
Example 194. N-;2-r4-Chloro-N-(4-hydroxybenzyl) cinnamvlaminolethvlE-5-isoquinolinesulfonamide 0.2 g of the product of Example 172 and 0.13 g of p-hydroxybenzaldehyde were dissolved in 10 ml of methanol, to the solution were added 60 mg of sodium cyanoborohydride and two drops of acetic acid, and the mixture was stirred for 2 days at a room temperature.
The reaction mixture was concentrated under a reduced pressure, and after adding a saturated sodium chloride aqueous solution, extracted three times with 20 ml of ethyl acetate. The extracts were combined, washed with sodium chloride, dried over magnesium sulfate, filtered and concentrated under a reduced pressure. The resulting residue was purified on a silica gel column (silica gel 10 g, eluant: 2% methanol in chloroform), to obtain 150 mg of the title compound in a colorless amorphous form.
1H-NMR (CDCl3 , 6 ppm): 2.50 (2H, brt), 2.90 (2H, brt), 3.10 (2H, d, J = 6.6 Hz), 3.35 (2H, s), 6.06 (1H, dt, J = 15.6, 6.6 Hz), 6.35 (1H, d, J = 15.9 Hz), 6.75 (2H, d, J = 8.5 Hz), 6.97 (2H, d, J = 8.5 Hz), 7.30 (4H, s), 7.65 (1H, t, J = 8.0 Hz), 8.15 (1H, d, J = 8.0 Hz), 8.37 - 8.41 (2H, m), 8.63 (1H, d, J = 6.0 Hz), 9.32 (1H, s).
Example 195. 2-Methyl-5-rF2-(4-chloro-N,N dimethYlcinnamvlammonio)ethYliaminosulfonYlE isoquinolium diiodide 83 mg of the product of Example 172 was dissolved in 2.0 ml of dimethylformamide, to the solution was added 1.0 ml of methyl iodide, and the mixture was stirred for 4 hours at a room temperature. Excess of methyl iodide and dimethylformamide were evaporated off under a reduced pressure, and the resulting residue was crystallized from 5 my of a mixture of methanol/chloroform (1:5). Crude crystals thus obtained was recrystallized from 10 ml of a mixture of methanol/chloroform (1:5), to obtain 78 mg of the title compound as light yellow crystals.
Melting point: 199 - 2000C; 1H-NMR (DMSO-d6 , 6 ppm): 3.09 (6H, s), 3.43 (4H, brs), 4.16 (2H, d, J = 7.0 Hz), 4.53 (3H, s), 6.48 (lH, dt, J = 15.9, 7.0 Hz), 6.89 (1H, d, J = 15.9 Hz), 7.58 (2H, dm, J = 9.4 Hz), 7.61 (2H, dm, J = 9.4 Hz), 8.21 (lH, t, J = 7.9 Hz), 8.72 - 8.78 (2H, m), 8.90 - 8.99 (3H, m), 10.22 (lH, brs).
Example 196. 2-Methyl-5-;N-methyl-N-F 2-(4-chloro- N,N-dimethylcinnamolammonio ! ethvllaminosulfonvl} isoquinolium iodide 83 mg of the product of Example 172 was dissolved in 2.0 ml of dimethylformamide, to the solution were added 1.0 ml of methyl iodide and 83 mg of anhydrous sodium carbonate, and the mixture was stirred for 4 hours at a room temperature. Excess methyl iodide and dimethylformamide were evaporated off under a reduced pressure, and after adding 10 ml of a mixture of methanol/chloroform (1:5) the mixture was stirred, and then filtered to remove insoluble matter. The filtrate was concentrated under a reduced pressure, and to the concentrate was added 10 ml of a mixture of methanol/chloroform (1:5) to precipitate an insoluble matter, which was then filtered off.This concentration and filtration procedure was twice repeated, to obtain 145 mg of the title compound in yellow amorphous form.
1H-NMR (DMSO-d6 , s ppm): 2.97 (3H, s), 3.15 (6H, s), 3.60 - 3.70 (2H, m), 3.70 - 3.80 (2H, m), 4.21 (2H, d, J = 7.3 Hz), 4.54 (3H, s), 6.54 (1H, dt, J = 15.6, 7.3 Hz), 6.93 (1H, d, J = 15.6 Hz), 7.48 (2H, brd, J = 8.5 Hz), 7.63 (2H, brd, J = 8.5 Hz), 8.23 (1H, t, J = 7.9 Hz), 8.75 - 8.85 (3H, m), 8.99 (lH, d, J = 7.1 Hz), 10.22 (1H, brs).
The isoquinoline compounds other than that of Example 174 can be treated with an excess amount of methyl iodide in dimethylformamide, as described in this Example, to obtain corresponding compounds wherein the nitrogen atom on the isoquinoline ring has been methylated to a quaternary nitrogen atom.
Example 197. N- r 2-(4-Chlorocinnamvlamino!eths11-5- isoauinolinesulfonamide dihydrochioride 2.00 g of the product of Example 172 was suspended in 20 ml of methanol, the suspension was made to a clear solution by adding 1 ml of concentrated hydrochloric acid, and stirred for 10 minutes with ice cooling to form crystals. The crystals were collected by filtration, and recrystallized from a mixture of 20 ml of methanol and 3 ml of water to obtain 1.65 g of the corresponding dihydrochloride as colorless crystals.
Melting point: 205 - 2080C; 1H-NMR (DMSO-d6 , E ppm): 2.90 - 3.05 (2H, m), 3.10 - 3.20 (2H, m), 3.65 - 3.75 (2H, m), 4.3 - 4.9 (br), 6.33 (1H, dt, J = 16.1, 7.1 Hz), 6.76 (1K, d, J = 16.1 Hz), 7.45 (4H, s), 8.00 (lH, dd, J = 8.3, 7.5 Hz), 8.54 (1H, dd, J = 7.5, 1.2 Hz), 8.64 (1H, brd, J = 8.3 Hz), 8.71 (1H, brd, J = 6.4 Hz), 8.80 (1H, br), 8.82 (1H, d, J = 6.4 Hz), 9.39 (1H, brs), 9.79 (lH, brs).
Example 198. N-[2-(&alpha;-Methylcinnamylamino)ethyl]-5- isoauinolinesulfonamide dihydrochloride The same procedure as described in Example 194 was repeated except that the product of Example 154 was used as a starting material, to obtain the corresponding dihydrochloride as colorless crystals.
Melting point: 80 - 850C; 1H-NMR (DMSO-d6 , 6 ppm): 1.40 (3H, d, J = 6.5 Hz), 2.89 (2H, m), 3.16 (2H, brt, J = 6.5 Hz), 3.91 (1H, m), 5.0 - 6.0 (br), 6.19 (1H, dd, J = 16.1,.
7.5 Hz), 6.71 (1H, d, J = 16.1 Hz), 7.39 (5H, m), 7.97 (1H, dd, J = 8.0, 7.6 Hz), 8.51 - 8.82 (5H, m), 9.44 (2H, br), 9.76 (1H, d, J = 1.0 Hz) Example 199. N-F2-(4-Chloro-a-methylcinnamyl- amino ethyl 1 -5-isopuinolinesulfonamide dihydro chloride The same procedure as described in Example 194 was repeated except that the product of Example 153 was used as a starting material, to obtain the corresponding dihydrochloride as a white hygroscopic powder.
1H-NMR (DMSO-d6 , 6 ppm): 1.40 (3H, d, J = 6.5 Hz), 2.85 - 3.96 (2H, m), 3.10 - 3.20 (2H, m), 3.80 - 4.00 (1H, m), 5.1 - 6.1 (br), 6.23 (1H, dd, J = 15.9, 8.5 Hz), 6.72 (1H, d, J = 15.9 Hz), 7.44 (4H, s), 7.99 (1H, dd, J = 8.2, 7.4 Hz), -8.54 (1H, ad, J = 7.4, 1.2 Hz), 8.64 (1H, brd, J = 8.2 Hz), 8.72 (1H, brd, J = 6.4 Hz), 8.80 (1H, br), 8.82 (1H, d, J = 6.4 Hz), 9.48 (2H, brs), 9.80 (lH, brs).
Example 200. N- r 2- (4-Bromocinnamylamino )ethyl 1-5- isoquinolinesulfonamide dihydrochloride The same procedure as described in Example 194 was repeated except that the product of Example 176 was used a starting material, to obtain the corresponding dihydrochloride as colorless crystals.
Melting point: 195 - 2000C; 1H-NMR (DMSO-d6 , 6 ppm): 2.90 - 3.10 (2H, brs), 3.2 - 3.3 (2H, m), 3.65 - 3.75 (2H, brs), 6.35 (1H, dt, J = 16.0, 7.1 Hz), 6.76 (lH, d, J = 16.0 Hz), 7.38 (2H, d, J = 8.5 Hz), 7.57 (2H, d, J = 8.5 Hz), 8.10 (1H, t, J = 7.6 Hz), 8.67 (lH, d, J = 7.6 Hz), 8.78 (lH, d, J = 7.6 Hz), 8.90 (lH, brs), 9.05 (lH, brs), 10.0 (lH, s).
Example 201. N- ( 2-Cinnamylaminoethyl ) -5- isoquinolinesulfonamide.1/2 fumarate 303 mg of the product of Example 173 was dissolved in 5 ml of ethyl acetate, to the solution was added a solution of 89 ml of fumaric acid in 2 ml of methanol at a room temperature, and the mixture was stirred for 30 minutes to from crystals, which was then collected by filtration and washed with ethyl acetate to obtain 312 mg of the corresponding 1/2 fumarate as colorless crystals.
Melting point: 153 - 156eC; 1H-NMR (DMSO-d6 , s ppm): 2.69 (2H, brt, J = 6.3 Hz), 3.00 (2H, brt, J = 6.3 Hz), 3.34 (2H, brd, J = 6.1 Hz), 5.0 - 8.0 (3H, br), 6.16 (lH, dt, J = 16.0, 6.1 Hz), 6.51 (lH, d, J = 16.0 Hz), 6.54 (lH, s), 7.23 7.3 (2H, m), 7.34 - 7.40 (3H, m), 7.82 (1H, dd, J = 8.1, 7.5 Hz), 8.36 (lH, dd, J = 7.5, 1.0 Hz), 8.42 (1H, brd, J = 8.1 Hz), 8.44 (lH, brd, J = 6.1 Hz), 8.69 (lH, d, J = 6.1 Hz), 9.47 (lH, d, J = 1.0 Hz).
Example 202. N-F 2-(a-Methylcinnamylamino)ethyl 1-5- isoauinolinesulfonamide 1/2 fumarate The same procedure as described in Example 198 was repeated except that the product of Example 154 was used as a starting material.to obtain the corresponding 1/2 fumarate as colorless crystals.
Melting point: 162 - 1670C; 1H-NMR (DMSO-d6 , 6 ppm): 1.02 (3H, d, J = 6.4 Hz), 2.49 (2H, brt, J = 6.6 Hz), 2.5 - 5.7 (3H, br), 2.93 (2H, brt, J = 6.6 Hz), 3.17. (1H, dd, J = 7.9, 6.4 Hz), 5.91 (1H, dd, J = 16.1, 7.9 Hz), 6.35 (1H, d, J = 16.1 Hz), 6.55 (1H, s), 7.32 (5H, m), 7.79 (1H, dd, J = 8.0, 7.3 Hz), 8.34 (1H, dd, J = 7.3, 1.2 Hz), 8.39 (1H, brd, J = 8.0 Hz), 8.43 (lH, brd, J = 6.1 Hz), 8.69 (1H, d, J = 6.1 Hz), 9.45 (lH, d, J = 1.0 Hz).
Example 203. N- F 2- (4-Chloro-a-methylcinnamyl- amino)ethyl] -5-isoquinolinesulfonamide L-(+)- tartrate 6.60 g of the product of Example 153 was dissolved in 50 ml of ethyl acetate, to the solution was added a solution of 2.38 g of L-(+)-tartaric acid in methanol to form crystals, which was then collected by filtration and washed with ethyl acetate to obtain the corresponding L-(+)-tartrate as colorless crystals.
Melting point: 125 - 1300C; 1H-NMR (DMSO-d6 , 6 ppm): 1.14 (3H, d, J = 6.3 Hz), 2.64 (2H, brt, J = 6.5 Hz), 2.98 (2H, brt, J = 6.5 Hz), 3.45 (1H, dd, J = 8.0, 6.3 Hz), 3.5 - 4.7 (6H, br), 4.13 (2H, s), 6.03 (lH, dd, J = 15.9, 8.0 Hz), 6.49 (1H, d, J = 15.9 Hz), 7.41 (4H, s), 7.81 (1H, dd, J = 8.0, 7.3 Hz), 8.36 (lH, brd, J = 8.0 Hz), 8.41 (1H, d, J = 6.1 Hz), 8.42 (1H, brd, J = 7.3 Hz), 8.69 (1H, d, J = 6.1 Hz), 9.46 (1H, d, J = 1.0 Hz).
Example 204. N-(2-Aminoethvl -N-( 2-cinnamvlamino- ethyl -5-isopuinolinesulfonamide trihydrochloride To a solution of 1.10 g of the amorphous compound obtained in Example 173, 1.18 g of triphenylphosphine and 0.73 g of 2-(tert-butoxycarbonylamino)ethanol in 15 ml of tetrahydrofuran, was added dropwise a solution of 0.78 g of diethyl azodicarboxylate in 5 ml of tetrahydrofuran for 15 ml with ice cooling, and the mixture was stirred for 4 hours at a room temperature.
After again ice-cooling, to the reaction mixture was added 0.39 g of triphenylphosphine, and added dropwise a solution of 0.26 g of diethylazodicarboxylate in 3 ml of tetrahydrofuran, and the reaction mixture was stirred at a room temperature for one hour; The reaction mixture was concentrated under a reduced pressure, and resulting residue was applied to a silica gel column and eluted with chloroform/methanol (19:1), to obtain 0.99 g of a light orange amorphous product. The product was dissolved in 20 ml of methanol, to the solution was added 7.7 ml of 4 N hydrochlonic acid in ethyl acetate, and the mixture was stirred at a room temperature for 3 hours. The reaction mixture was evaporated to remove the solvent under a reduced pressure, and thereto was added ethyl acetate to form a solid.The solid was collected by filtratoin, washed with ethyl acetate and n-hexane, and dried under a reduced pressure to obtain 0.97 g of the title compound as a colorless hygroscopic powder.
NMR (D20) 5 ppm: 3.2 - 3.5 (4H, m), 3.7 - 4.0 (6H, m), 6.1 - 6.3 (1H, m), 6.82 (1H, d, J = 15.9 Hz), 7.44 (5H, s), 8.15 (1H, t, J = 7.6 Hz), 8.1 - 8.3 (3H, m), 9.09 (1H, d, J = 7.0 Hz), 9.7 (lH, s).
Example 205. N-(4-Aminobutyl)-N-F2- (4-chloro- cinnamvlamino ! ethvll-5-isoquinolinesulfonamide trihydrochlorides.
To a solution of 0.4 g of the crystals obtained in Example 172. 0.226 g of 4-(tert-butoxycarbonylamino)butanol and 0.445 g of triphenylphosphine in 5 ml of tetrahydrofuran, was added a solution of 0.295 g of diethyl azodicarboxylate in 2 ml of tetrahydrofuran with stirring under ice cooling. The mixture was allowed to stand at room temperature, and evaporated under a reduced pressure to obtain a residue, which was then applied to a silica gel column and eluted with methanol/chloroform (2:98) to obtain 0.28 g of oil. To a solution of the oil in 1 ml of methanol, as added 4 N hydrochloric acid/ethyl acetate to form a precipitate, which was then collected by filtration, washed with ethyl acetate and dried to obtain 0.2 g of the title compound as a colorless powder.
NMR (D2O) a ppm: 1.70 (4H, brs), 2.95 (2H, m), 3.30 (2H, m), 3.55 (2H, m), 3.77 (2H, m), 3.89 (2H, dd, J = 7.3 Hz), 6.15 (lH, dt, J = 15.8, 7.3 Hz), 6.76 (1H, d, J = 15.8 Hz), 7.35 (4H, s), 8.11 (lH, t, J = 8.0 Hz), 8.6 - 8.8 (2H, m), 8.98 (lH, d, J = 7.0 Hz), 9.75 (1H, s).
Example 206. N- F 2- ( 4-Chloro-N-methylcinnamyl- amino )ethyll-N-F 2- (4-piperidyl)ethyll-5-iso- cuinolinesulfoneamide To a solution of 0.39 g of the amorphous compound obtained in Example 190, 0.145 g of 4-piperidinethanol and 0.265 g of triphenylphosphine in 5 ml of tetrahydrofuran, was added a solution of 0.245 g of diethyl azodicarboxylate in 2 ml of tetrahydrofuran with ice cooling, and the mixture was stirred for one hour at a room temperature and evaporated to remove the solvent under a reduced pressure. After an addition of 30 ml of ethyl acetate, the mixture was extracted three times with 5 ml of 1 N hydrochloric acid. The extract was alkalized with sodium bicarbonate and extracted three times with 10 ml of ethyl acetate. The organic layer was dried over magnesium sulfate and evaporated to remove the solvent.The resulting residue was applied to an almina chromatographic column and eluted with 1% methanol in chloroform to obtain 180 mg of the title compound as colorless oil.
NMR (CDC13) 6 ppm: 0.87 - 1.05 (2H, m), 1,30 1.45 (5H, m), 1.85 (1H, brs), 2.21 (3H, s), 2.33 (2H, m), 2.51 (2H, t, J = 7.6 Hz), 2.89 (2H, m), 3.08 (2H, d, J = 6.6 Hz), 3.30 (2H, t, J = 7.8 Hz), 3.42 (2H, t, J = 7.3 Hz), 6.09 (1H, dt, J = 15.8, 6.6 Hz), 6.41 (lH, d, J = 15.8 Hz), 7.26 (4H, s), 7.65 (1H, dd, J = 8.0, 8.6 Hz), 8.15 (1H, d, J = 8.0 Hz), 8.4 (1H, d, J = 8.6 Hz), 8.40 (1H, d, J = 6.1 Hz), 8.67 (lH, d, J = 6.1 Hz), 9.31 (1H, s).
The oil thus obtained was dissolved in 1 ml of methanol, and thereto were added 0.3 ml of 4 N hydrochlonic acid in ethyl acetate and then 30 ml of ether, to obtain the corresponding trihydrochloride as colorless powder.
Example 207. N- r 2- (4-Chloro-N-methylcinnamyl- amino ? ethyl 1-N-F 2-morpholinoethyl ) -5-isopuinoline- sulfonamide trihydrochloride To a solution of 1 g of the amorphous compound obtained in Example 190, 0.377 g of 2-N-morpholinoethanol and 1.25 g of triphenylphosphine in 5 ml of tetrahydrofuran, was added dropwise a solution of 0.835 g of diethyl azodicarboxylate in 2 ml of tetrahydrofuran with stirring under ice cooling, and the mixture was stirred for 2 hours. After evaporating off the solvent under a reduced pressure, to the residue was added 20 ml of ethyl acetate, and the mixture was extracted three times with 10 ml of 1 N hydrochloric acid. The extract was alkalized with sodium bicarbonate and extracted three times with 10 ml of ethyl acetate.
The extract was dried over magnesium sulfate and evaporated under a reduced pressure to remove the solvent. The resulting residue was applied to a silica gel column and eluted with methanol/ethyl acetate (10:90) to obtain an oil.
NMR (CDC13) 6 ppm: 2.18 (3H, s), 2.28 - 2.33 (4H, m), 2.4 (2.6 (4H, m), 3.07 (2H, d, J = 6.6 Hz), 3.4 3.6 (8H, m), 6.07 (lH, dt, J = 15.9, 6.6 Hz), 6.40 (lH, d, J = 15.9 Hz), 7.26 (4H, s), 7.63 (1H, dd, J = 8.0, 7.1 Hz), 8.14 (lH, d, J = 8.0 Hz), 8.42 (1H, d, J = 7.1 Hz), 8.42 (1H, d, J = 7.1 Hz), 8.42 (1H, d, J = 6.1 Hz), 8.67 (1H, d, J = 6.1 Hz), 9.31 (1H, s).
The oil thus obtained was dissolved 4 ml of methanol, and was added in 2 ml of 4 N hydrochloric acid in ethyl acetate, and the solvent was evaporated to remove the solvent under a reduced pressure, the resulting product was recrystallized from ethanol to obtain 0.67 g of the title compound as colorless crystals.
Melting point: 172 - 1760C; NMR (D20) 6 ppm: 3.04 (3H, s), 3.2 - 3.6 (8H, m), 3.8 - 4.1 (lOH, m), 6.18 (1H, dt, J = 15.9, 7.0 Hz), 6.76 (1H, d, J = 15.9 Hz), 7.22 (4H, s), 8.09 (lH, dd, J = 7.6, 8.2 Hz), 8.52 (1H, d, J = 7.6 Hz), 8.65 - 8.75 (2H, m), 8.87 (1H, d, J = 7.0 Hz), 9.74 (1H, s).
Example 208. N- F 2- (4-Chloro-N-methylcinnamyl- amino ) ethvl l-N-( 2-PiPeridinoethvl -5-isocruinoline- sulfonamide To a solution of 0.39 g of the amorphous compound obtained in Example 190, 0.145 g of l-piperidinethanol and 0.369 g of triphenylphosphine in 5 ml of tetrahydrofuran, was added dropwise a solution of 0.245 g of diethyl azodicarboxylate in 2 ml of tetrahydrofuran with stirring under ice cooling.. The mixture was stirred for 2 hours and evaporated to remove the solvent under a reduced pressure, and resulting residue dissolved in 30 ml of ethyl acetate and extracted three times with 10 ml of 1 N hydrochloric acid.The aqueous layer was alkallized with sodium bicarbonate and extracted three times with 10 ml of ehtyl acetate, and the organic extract was dried over magnesium sulfate and evaporated to remove the solvent at a reduced pressure. The resulting residue was applied to a silica gel column and eluted with 2% methanol in chloroform, to obtain 0.37 g of the title compound as a colorless oil.
NMR (CDC13) 6 ppm: 1.3 - 1.5 (6H, m), 2.20 (3H, s), 2.20 - 2.30 (4H, m), 2.39 (2H, t, J = 7.1 Hz), 2.55 (2H, t, J = 7.1 Hz), 3.08 (2H, d, J = 6.8 Hz), 3.46 (4H, q, J = 7.1 Hz), 6.09 (1H, dt, J = 15.9, 6.8 Hz), 6.40 (1H, d, J = 15.9 Hz), 7.25 (4H, s), 7.63 (1H, dd, J = 7.3, 8.1 Hz), 8.14 (lH, d, J = 8.1 Hz), 8.43 (1H, d, J = 7.3 Hz), 8.43 (lH, d, J = 6.1 Hz), 8.67 (1H, d, J = 6.1 Hz), 9.31 (1H, s).
To a solution of above-obtained oil in 3 ml of methanol, was added 0.5 ml of 4 N hydrochloric acid in ethyl acetate, and the whole was evaporated to remove the solvent under a reduced pressure. To the concentrate was added ether to form powder, which was then collected by filtration to obtain 0.35 g of the corresponding trihydrochloride as a colorless powder.
NMR (D20) 6 ppm: 1.3 - 2.0 (6H, m), 2.8 - 3.0 (2H, m), 3.05 (3H, s), 3.3 - 3.6 (6H, m), 3.8 - 4.1 (6H, m), 6.25 (1H, dt, J = 15.8, 8.0 Hz), 6.80 (1H, d, J = 15.8 Hz), 7.25 (4H, s), 8.13 (1H, t, J = 8.0 Hz), 8.60 (1H, d, J = 8.0 Hz), 8.68 - 8.78 (2H, m), 8.95 (1H, d, J = 7.0 Hz), 9.70 (1H, s).
Example 209. N-F2-(4-Chloro-N-methylcinnamyl- amino ) ethyl 1-N- (2-dimethylaminoethyl ) -5-iso- auinolinesulfonamide To a solution of 1.0 g of the amorphous compound obtained in Example 190, 0.267 g of 2-dimethylamino ethanol and 0.982 g of triphenylphosphine in 5 ml of tetrahydrofuran, was added dropwise a solution of 0.652 g of diethyl azodicarboxylate in 2 ml of tetrahydrofuran with stirring under ice cooling. After 2 hours, the reaction mixture was concentrated under a reduced pressure to remove tetrahydrofuran, and resulting residue was dissolved in 10 ml of ethyl acetate and extracted three times with 10 ml of l N hydrochloric acid.The aqueous layer was alkalized with sodium bicarbonate and extracted three times with 10 ml of ethyl acetate, and the organic layer was dried over magnesium sulfate and evaporated to removed the solvent under a reduced pressure. The resulting residue was then applied to a silica gel column and eluted with 3% methanol in chloroform, to obtain 0.77 g of the title compound as a colorless oil.
NMR (CDC13) , 6 ppm: 2.11 (6H, s), 2.20 (3H, s), 2.38 (2H, t, J = 7.3 Hz), 2.54 (2H, t, J = 7.3 Hz), 3.08 (2H, d, J = 6.6 Hz), 3.38 - 3.50 (4H, m), 6.08 (1H, dt, J = 15.8, 6.6 Hz), 6.40 (1H, d, J = 15.8 Hz), 7.26 (4H, s), 7.63 (1H, dd, J = 8.1, 7.5 Hz), 8.14 (lH, d, J = 8.1 Hz), 8.40 - 8.45 (2H, m), 8.68 (1H, d, J = 6.1 Hz), 9.31 (1H, s).
To a solution of the oil thus obtained in 5 ml of methanol was added 1.4 ml of 4 N hydrochloric acid in ethyl acetate, and after evaporating off the solvent under a reduced pressure, to the resulting concentrate was added ether to form powder, which was then collected by filtration and dried to obtain 0.7 g of the corresponding trihydrochloride as a powder.
NMR (D2O) 6 ppm: 2.96 (6H, s), 3.03 (3H, s), 3.4 3.6 (4H, m), 3.9 - 4.1 (6H, m), 6.17 (1H, dt, J = 15.9, 7.0 Hz), 6.73 (1H, d, J = 15.9 Hz), 7.19 (4H, s), 8.01 (1H, t, J = 8.0 Hz), 8.54 (1H, d, J = 8.0 Hz), 8.70 (2H, m), 8.91 (1H, d, J = 8.0 Hz), 9.78 (1H, s).
Example 210. N-(2-Piperidinoethol)-N- r 2-(N-methyl- cinnamylaminoBetholl-5-isoquinolinesulfonamide The amorphous product obtained in Example 173 was treated according to the procedure described in Example 190, to obtain N-[2-(N-methylcinnamylamino)ethyl]-5-isoquinolinesulfonamide.
NMR (CDC13) 6 ppm: 1.95 (3H, s), 2.37 (2H, t, J= 5.7 Hz), 2.93 - 3.00 (4H, m), 6.00 (lH, dt, J = 15.8, 6.6 Hz), 6.38 (1K, d, J = 15.8 Hz), 7.31 (SH, s), 7.68 (1H, dd, J = 8.3, 7.3 Hz), 8.18 (1H, d, J = 8.3 Hz), 8.43 - 8.47 (2H, m), 8.69 (lH, d, J = 6.1 Hz), 9.34 (1H, s).
To a solution of 0.476 g of the above compound, 0.193 g of 1-piperidinethanol and 0.524 g of triphenylphosphine in 5 ml of tetrahydrofuran, was added a solution of 0.348 g of diethyl azodicarboxylate in 2 ml of tetrahydrofuran with stirring under ice cooling, and the mixture was allowed to stand for 3 hours and evaporated to remove the solvent under a reduced pressure. To the concentrate was added 30 ml of ethyl acetate, and the mixture was extracted three times with 10 ml of 1 N hydrochloric acid. The extract was alkalized with sodium bicarbonate and extracted three times with 10 ml of ethyl acetate. The ethyl acetate solution was dried over magnesium sulfate and evaporated under a reduced pressure to remove the solvent. The resulting residue was applied to a silica gel column and eluted with 5% methanol in chloroform, to obtain 0.44 g of the title compound as colorless oil.
NMR (CDC13) s ppm: 1.3 - 1.5 (6H, m), 2.20 (3H, s), 2.20 - 2.30 (4H, m), 2.41 (2H, t, J = 6.8 Hz), 2.53 (2H, t, J = 6.3 Hz), 3.09 (2H, d, J = 6.6 Hz), 3.4 - 3.55 (4H, m), 6.10 (1H, dt, J = 15.8, 6.6 Hz), 6.45 (1H, d, J = 15.8 Hz), 7.2 - 7.4 (5H, m), 7.61 (1H, dd, J = 8.0, 7.5 Hz), 8.11 (1H, d, J = 8.0 Hz), 8.4 - 8.5 (2H, m), 8.66 (1H, d, J = 6.1 Hz), 9.29 (1H, s).
To the oil thus obtained in 5 ml of methanol was added 0.8 ml of 4 N hydrochloric acid in ethyl acetate, and the solution was evaporated to remove the solvent under a reduced pressure. A precipitate obtained by addition of 50 ml of ether was collected by filtration and dried to obtain 0.4 g of the corresponding trihydrochloride as a colorless powder.
NMR (D2O) 6 ppm: 1.6 - 2.0 (6H, m), 2.7 - 2.9 (2H, m), 3.04 (3H, s), 3.4 - 3.6 (6H, m), 3.9 - 4.1 (6H, m), 6.25 (1H, dt, J = 15.8, 8.0 Hz), 6.86 (1H, d, J = 15.8 Hz), 7.40 (5H, s), 8.14 (1H, t, J = 8.0 Hz), 8.6 - 8.7 (3H, m), 9.0 (1H, d, J = 7.0 Hz), 9.72 (1H, s).
Example 211. N-Anisol-N- r 2-(4-chlorocinnamyl- aminoNethYll-5-isoquinolinesulfonamide 0.4 g of the crystals obtained in Example 172, 0.276 g of anisyl alcohol and 0.524 g of triphenylphosphine were dissolved in 10 ml of tetrahydrofuran, and to the solution was added dropwise a solution of 0.404 g of diisopropyl azodicarboxylate in 2 ml of tetrahydrofuran with stirring under ice cooling. The reaction mixture was warmed to a room temperature and was allowed to stand overnight and then evaporated under a reduced pressure to remove the solvent. The resulting residue was dissolved in 30 ml of ethyl acetate, and the mixture was extracted twice with 30 ml of 1 N hydrochloric acid. The extract was alkalized with sodium bicarbonate and extracted twice with 30 ml of ethyl acetate.The ethyl acetate solution was washed with water, dried over magnesium sulfate and evaporated under a reduced pressure to remove the solvent. The resulting residue was applied to a silica gel column and eluted with 1% methanol in chloroform, to obtain 0.22 g of the title compound as a colorless oil.
NMR (CDC13) 6 ppm: 2.61 (2H, t, J = 6.6 Hz), 3.14 (2H, d, J = 6.1 Hz), 3.34 (2H, t, J = 6.6 Hz), 3.74 (3H, s), 4,43 (2H, s), 6.0 (lH, dt, J = 15.9, 6.1 Hz), 6.30 (1H, d, J = 15.9 Hz), 6.75 (2H, d, J = 8.8 Hz), 7.08 (2H, d, J = 8.8 Hz), 7.26 (4H, s), 7.66 (1H, dd, J = 8.3, 7.3 Hz), 8.17 (lH, brd, J = 8.3 Hz), 8.39. - 8.49 (2H, m), 8.69 (1H, d, J = 6.1 Hz), 9.3 (lH, s).
Example 212. N-F 2- ( 4-Chlorocinnamylamino )ethyl 1 - N-phenethyl-S-isoouinolinesulfonamide The procedure described in Example 211 was repeated except that 0.146 g of phenethyl alcohol was used in place of anisyl alcohol, to obtain 0.37 g of the title compound as a colorless oil.
NMR (CDC13) 6 ppm: 1.4 (1H, brs), 2.75 - 2.90 (4H, m), 3.27 (2H, d, J = 6.1 Hz), 3,4 - 3.6 (4H, m), 6.10 (1H, dt, J = 15.9, 6.1 Hz), 6.39 (lH, d, J = 15.9 Hz), 6.59 - 7.05 (2H, m), 7.1 - 7.2 (3H, m), 7.26 (4H, s), 7.65 (1H, dd, J = 8.3, 7.6 Hz), 8.15 (lH, d, J = 8.3 Hz), 8.38 (2H, t, J = 6.1 Hz), 8.63 (1H, d, J = 6.1 Hz), 9.28 (1H, s).
Example 213. N-Benzyl-N-F2-(4-chlorocinnamyl- amino ) ethyl 1 -5-isomiinolinesulfonamide The procedure described in Example 211 was repeated except that 0.162 g of benzyl alcohol was used in place of anisyl alcohol, to obtain 0.3 g of the title compound as a colorless oil.
NMR (CDC13) 6 ppm: 2.0 (lH, brs), 2.6 (2H, t, J = 6.6 Hz), 3.15 (2H, d, J = 6.1 Hz), 3.40 (2H, t, J = 6.6 Hz), 4.50 (2H, s), 6.0 (1H,'dt, J = 15.8, 6.1 Hz), 6.30 (1H, d, J = 15.8 Hz), 7.15 - 7.25 (9H, m), 7.66 (1H, dd, J = 8.0, 7.6 Hz), 8.16 (1H, d, J = 8.0 Hz), 8.4 - 8.5 (2H, m), 8.70 (1H, d, J = 6.1 Hz), 9.31 (1H, 5).
Example 214. N- 2 - 2-(4-ChlorocinnamYlamino)ethvll- N-methyl-5-isoquinolinesulfonamide The procedure described in Example 211 was repeated except that 48 mg of methanol was used in place of anisyl alcohol, to obtain 0.3 g of the title compound as a colorless oil.
NMR (CDC13) 6 ppm: 1.5 (1H, brs), 2.86 (2H, t, J = 6.2 Hz), 2.88 (3H, s), 3.3 - 3.4 (4H, m), 6.15 (1H, dt, J = 15.8, 6.1 Hz), 6.43 (1H, d, J = 15.8 Hz), 7.27 (4H, s), 7.69 (lH, dd, J = 8.3, 7.3 Hz), 8.18 (1H, d, J = 8.3 Hz), 8.38 (1H, d, J = 7.3 Hz), 8.50 (1H, d, J = 6.3 Hz), 8.67 (1H, d, J = 6.3 Hz), 9.31 (lH, s).
Reference Example 45. N- (3 ,4-Dimethoxyphenyl ) -5- isoquinolinesulfonamide 3.06 g of 3,4-dimethoxyaniline was dissolved in 30 ml of pyridine, to the solution was added in small portions 5.28 g of 5-isoquinolinesulfonyl chloride.HCl with stirring under ice cooling, and the mixture was stirred for 30 minutes, and further stirred at a room temperature overnight. After evaporating off the pyridine under a reduced pressure and additing 20 ml of water, the mixture was extracted twice with 50 ml of chloroform/isopropanol (10:1). The extract was dried over magnesium sulfate and evaporated under a reduced pressure to remove the solvent. To the resulting residue was added 20 ml of benzene/chloroform (3:1), and the mixture was slightly warmed and collected to obtain 5.64 g of the title compound as colorless crystals.
Melting point: 195 - 1970C; NMR (CDC13) s ppm: 3.67 (3H, s), 3.77 (3H, s), 6.3 (1H, dd, J = 8.5, 2.7 Hz), 6.5 - 6.6 (3H, complex), 7.61 (1H, t, J = 8.3 Hz), 8.2 (1H, d, J = 8.3 Hz), 8.3 (1H, dd, J = 1.3, 7.3 Hz), 8.4 (1H, d, J = 6.1 Hz), 8.7 (1H, d, J = 6.4 Hz), 9.36 (1H, d, J = 1.3 Hz).
Reference Example 46. N-(3 4-DimethoxvPhenvl)-N- (2-phthalimidethvl!-5-isoquinolinesulfonamide 500 mg of the crystals obtained in Reference Example 45 was dissolved in 7 ml of dimethylformamide and 4 ml of tetrahydrofuran, to the solution was added 70 mg of 60% sodium hydride with stirring under ice cooling, and the mixture was stirred for 20 minutes, and fter adding 406 mg of bromoethylphthalimide, the whole was refluxed for 6 hours with stirring. After adding 10 ml of ice water, the reaction mixture was extracted with 30 ml of ethyl acetate, and the extract was dried over magnesium sulfate-and evaporated under a reduced pressure to remove the solvent.The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 320 mg of the title compound as colorless crystals.
Melting point: 197 - 2010C; NMR (CDC13) 6 ppm: 3.80 (3H, s), 3.88 (3H, s), 3.7 - 3.78 (2H, complex), 3.75 - 4.0 (2H, complex), 6.67 (1H, s), 6.68 (1H, s), 6.73 (1H, s), 7.57 (1H, t, J = 7.57 Hz), 7.73 (4H, s), 8.0 (1H, dd, J = 1.0, 8.3 Hz), 8.05 (lH, d, J = 7.3 Hz), 8.24 (1H, dd, J = 1.0, 7.57 Hz), 8.44 (1H, brd), 9.1 (1H, brs).
Reference Example 47. N-(3 ,4-Dimethoxvphenyl )- N-(2-aminoethvl)-5-isoquinolinesulfonamide 517 mg of the crystals obtained in Reference Example 46 was dissolved in 5 ml of methanol and 5 ml of chloroform, to the solution was added 60 mg of hydrozine hydrate, and the mixture was refluxed for 3 hours.
Crystallized insoluble matter was filtered off, and the filtrate was evaporated under a reduced pressure to remove the solvent. After an addition of 10 ml of ethyl acetate, the whole was filtered to remove the insoluble matter and then evaporated under a reduced pressure to yield 420 mg of the title compound obtained as a slightly yellow oil.
NMR (CDC13) 6 ppm: 2.76 (2H, t, J = 6.1 Hz), 3.60 (3H, s), 3.75 (2H, t, J = 6.1 Hz), 3.83 (3H, s), 6.48 (lH, s), 6.46 (lH, d, J = 9.2 Hz), 6.63 (1H, d, J = 9.2 Hz), 7.61 (1H, t, J = 7.5 Hz), 8.18 (1H, d, J = 8.0 Hz), 8.25 (1H, dd, J = 1.3, 8.3 Hz), 8.5 (lH, d, J = 6.1 Hz), 9.3 (1H, d, J = 1.3 Hz).
Example 215. N-(3,4-Dimethoxophenyl)-N- r 2-(4- chlorocinnamolamino)ethvll-5-isoquinoline- sulfonamide 320 mg of the oil obtained in Reference Example 47 was dissolved in 6 ml of dimethylformamide, to the solution were added 200 mg of potassium carbonate and 150 mg of p-chlorocinnamyl chloride, and the mixture was stirred overnight at a room temperature.
After adding 20 ml of water the reaction mixture. was extracted twice with 30 ml of chloroform. The extract was washed with a saturated sodium chlbride aqueous solution, dried over magnesium chloride and evaporated under a reduced pressure to remove the solvent. The resulting residue was applied to a silica gel column and eluted with chloroform/methanol (100:1) to obtain 90 mg of the title compound as colorless crystals.
NMR (CDC13) 6 ppm: 2.75 (2H, t, J = 6.1 Hz), 3,36 (lH, d, J = 6.1 Hz), 3.6 (3H, s), 3.74 (2H, d, J = 6.1 Hz), 3.82 (3H, s), 6.15 (1H, d, and dt, J = 15.6, 6.1 Hz), 6.42 (lH, d, J = 15.6 Hz), 6.5 (1H, s), 6.61 (lH, d, J = 8.1 Hz), 6.48 (lH, d, J = 6.1 Hz), 7.3 (4H, brs), 7.63 (1H, t, J = 8.1 Hz), 8.16 (lH, d, J = 6.1 Hz), 8.17 (1H, d, J = 8.1 Hz), 8.3 (1H, dd, J = 1.0, 6.1 Hz), 8.5 (1H, d, J = 6.1 Hz), 9.3 (1H, d, J = 1.0 Hz).
Example 216. N-2-rBis (4-chlorocinnamyl )aminol ethyl -5-i soquinolinesulfonamide In Example 215, prior to elution using chloroform/methanol, elution was carried out using chloroform to obtain 100 mg of the title compound in a colorless amorphous form.
NMR (CDCl3) E ppm: 2.66 (2H, t, J = 6.2 Hz), 3.25 (4H, d, J = 6.2 Hz), 3.52 (3H, s), 3.75 (2H, t, J = 6.2 Hz), 3.71 (3H, s), 6.1 (2H, d and t, J = 15.6, 6.2 Hz), 6.3 (1H, d, J = 5.6 Hz), 6.4 (1H, s), 6.4 (2H, d, J = 15.6 Hz), 6.45 (lH, d, J = 5.6 Hz), 7.3 (8H, s), 7.51 (1H, t, J = 8.1 Hz), 8.14 (lH, d, J = 6.1 Hz), 8.16 (1H, d, J = 8.1 Hz), 8.2 (lH, dd, J = 1.0, 6.1 Hz), 8.45 (lH, d, J = 6.1 Hz), 9.3 (lH, d, J = 1.0 Hz).
As described above, the following compounds were prepared.
Example 217. N- F 2-4-methoxy-a-methylcinnamylamino) -ethvll-5-isoauinolinesulfonamide.2HC1 Colorless amorphous form.
IR(KBr)cm : 3420, 3200-2300, 1720, 1605, 1345, 1280; UMR(D2O) s ppm: 1.59 (3H, d, J = 6.71 Hz), 3.19 (2H, brt.), 3.38 (2H, brt), 4.01 (3H.s), 4.16 (1H, m), 6.28 (1H, dd, J = 15.9, 8.9 Hz), 6.83 (lH, d, J = 15.9 Hz), 7.51 (2H, d, J = 8.4 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.10 (1H, brt), 8.64 (1H, d, J = 8.6 Hz), 8.76 (2H, brt), 8.98 (1H, d, J = 7.0 Hz), 9.72 (lH, s).
Example 218. N- r 2-(4-methoxvcarbonol-N-dimethY -cinnamvlaminoethvll-5-isoauinoline- sulfonamide. 2HC1 Colorless amorphous form.
IR(KBr)cm 1 = 3420, 3150-2300, 1715, 1605, 1345, 1285; NMR(D2O)Bppm = 1.59 (3H, a, J = 6.4 Hz), 2.94 (3H, s), 3.44 (4H, brs), 3.99 (3H, s), 4.31 (1H, m), 6.37 (1H, dd, J = 16.2, 8.7 Hz), 6.89 (1H, d, J = 16.2 Hz), 7.54 (2H, d, J = 8.1 Hz), 8.08 (2H, d, J = 8.1 Hz), 8.10 (1H, brt), 8.67 (lH, d, J = 8.5 Hz), 8,75 (2H, brt), 8.96 (1H, d, J = 7.0 Hz), 9.74 (lH, s).
Example 219. N- r 2-t4-methoxv-N,-dimethylcinnamvl -amino)ethvll-5-isoauinolinesulfonamide Colorless oil.
NMR (CDC13)appm: 1.07 (3H, d, J = 6.6 Hz), 1.85 (3H, s), 2.3-2.5 (2H, m), 2.91 (2H, t, J = 6.0 Hz), 2.95-3.10 (1H, m), 3.80 (3H, s), 5.86 (lH, dd, J = 6.0 Hz), 2.95-3.10 (lH, m), 3.80 (3H, s), 5.86 ((lH, dd, J = 16.1, 7.3 Hz), 6.24 (1H, d, J = 16.1 Hz), 6.84 (2H, d, J = 8.8 Hz), 7.24 (2H, d, J = 8.8 Hz), 7.68 (1H, dd, J = 7.3, 8.0 Hz), 8.20 (1H, a, J = 8.0 Hz), 8.4 - 8.5 (2H, m), 8.66 (1H, d, J = 6.1 Hz), 9.34 (1H, s).
ExamPle 220. N- (2-methylaminoethyl ) -N- F 2- Hz 4 (4-chloro N-methylcinnamYlaminossethol1-5-isoquinoline- sulfonamide Colorless oil.
NMR (CDO3)Sppm: 1.9-2.2 (1H, br), 2.23 (3H, s), 2.35 (3H, s), 2.59 (2H, t. J = 6.6 Hz), 2.80 (2H, t, J = 6.1 Hz), 3.12 (2H, a, J = 6.6 Hz), 3.4-5.5 (4H, m), 6.10 ((1H, dt, J = 15.9, 6.6 Hz), 6.44 ((1H, d, J = 15.9 Hz), 7.26 (4H, s), 7.66 (1H, t, J = 15.9, 6.6 Hz), 6.44 (1H, d, J = 15.9 Hz), 7.26 (4H, s), 7.66 (1H, t, J = 7.8 Hz), 8.17 (1H, d, J = 7.8 Hz), 8.40 (1H, d, J = 7.8 Hz), 8.46 (1H, d, J = 6.1 Hz), 8.67 (1H, d, J = 6.1 Hz), 9.32 (1H, s).
Example 221. N-( 2-methylaminoethyl )-N-F 2 (4-chloro-N-methylcinnamolaminoletho11-5- isoquinolinesulfonamide.3HCl Colorless amorphous form.
NMR(D2O)Sppm: 2.80 (3H, s), 3.05 (3H, s), 3.4-3.6 (4H, m), 3.9-4.1 (6H, m), 6.17 (1H, d.t, J = 15.9, 7.2 Hz), 6.74 (1H, d, J = 15.9 Hz), 7.18 (4H, s), 8.10 (1H, t, J = 7.9 Hz), 8.53 (1H, d, J = 7.9 Hz), 8.6-8.8 (2H, m), 8.93 (1H, d, J = 7.0 Hz), 9.77 (1H, s).
Example 222. N-2-hydroxsethyl)-N- r 2-(4-methoxy- N,P-dimethvlcinnamvlaminolethvll-5- isoauinolinesulfonamide Colorless amorphous form.
NMR(CDCl3)Eppm: 1.29 (3H, d, J = 6.6 Hz), 2.30 (3H, s), 2.7-3.0 (2H, m), 3.2-3.4(5H, m), 3.80 (3H, s), 3.8-3.9 (2H, m), 6.04 (1H, dd, J = 16.1, 7.8 Hz), 6.40 (1H, d, J = 16.1 Hz), 6.86 (2H, d, J = 16.1, 7.8 Hz), 6.40 (1H, d, J = 16.1 Hz), 6.86-(2H, d, J = 8.7 Hz), 7.32 (2H, d, J = 8.7 Hz), 7.32 (2H, d, J = 8.7 Hz), 7.68 (1H, dd, J = 8.0, 7.3 Hz), 8.19 (1H, d, J = 8.0 Hz), 8.26 (Ih, d, J = 7.3 Hz), 8.58 (1H, d, J = 6.1 Hz), 8.68 (1H, d, J = 6.1 Hz), 8.68 (1H, d, J = 6.1 Hz), 9.33 (1H, 5) Example 223.N-F 2- (methoxy)ethyll-N-F2-(N-methyl -4-methoxv-&alpha;-methvlcinnamolamino)ethyl]-5- isoauinolinesulfonamide Colorless oil NMR(CDCl3)Eppm: 1.12 (3H, d. J = 6.6 Hz), 2.18 (3H, s), 2.4-2.65 (2H, m), 3.1 (3H, s), 3.10-3.20 (1H, m), 3.35-3.60 (6H, m), 3.81 (3H, s), 5.93 (1H, d,d, J = 16.1, 7.3 Hz), 6.31 (1H, d, J = 16.1 Hz), 6.85 (2H, d, J = 8.7 Hz), 7.62 (1H, dd, J = 7.6, 8.1 Hz), 8.13 (1H, d, J = 8.1 Hz), 8.35-8.45 (2H, m) 8.67 (1H, d, J = 6.1 Hz), 9.30 (1H, 5).
Example 224. N-[2- (4-chlorocinnamylamino)ethyl] -N-(2-dimethylaminoethyl-5-isquinolinesulforn- amide. 3HCl Colorless amorphous form.
IR(KBr)cm-1. 1 3420, 2700, 1340, 1150, 840, 590 NMR(D20)6ppm: 2.99 (6H, s), 3.33 (2H, t, J = 6.8 Hz), 3.55 (2H, t, J = 6.8 Hz), 3.8-4.0 (6H, m), 6.18 (1H, dt, J = 16.2, 6.7 Hz), 6.76 (1H, d, J = 16.2 Hz), 7.32 (4H, s), 8.12 (1H, t, J = 8.0 Hz), 8.6-8.8(3H, m), 8.97 (lH, d, J = 7.0 Hz), 9.74 (1H, s).
Example 225. N-[2-(4-chlorocinnamylamino)ethyl) -N-(2-metholaminoethyl)-5-isquinolinesulfonamide Colorless amorphos form.
NMR(D2O)#ppm: 3.26 (2H, brt), 3.92 (4H, brt), 5.04 (2H, s), 6.1-6.3 (1H, m), 6.77 (1H, d, J = 15.6 Hz), 7.38 (4H, s), 7.68 (1H, t, J = 6.7 Hz), 8.0-8.3 (2H, m), 8.57 (1H, d, J = 5.8 Hz), 8.7- 8.9 (3H, m), 9.02 (lH, d, J = 7.3 Hz), 9.80 (1H, s).
Example 226. N-2-(4-chlorocinnamylamino)ethyl] -N-(2-pyridylmethy)-5-isoquinolinesulfonamide.
3HC1 Pale yellow amorphous form.
IR(KBr)cm : 3420, 2800, 1350, 1150, 590; NMR(D20)Eppm: 3.12 (2H, brt), 3.8-4.0 (4H, m), 4.96 (2H, s), 6.0-6.2 (1H, m), 6.70 (1H, d, J = 15.7 Hz), 7.37 (4H, brq), 7.93 (1H, t, J = 6.3 Hz), 8.16 (2H, brt), 8.54 (1H, d, J = 5.8 Hz), 8.61 (1H, d, J = 8.5 Hz), 8.7- 8.8 (2H, m), 8.83 (1H, s), 9.01 (1H, d, J = 6.7 Hz), 9.76 (1H, s).
Example 227. N- 2-(4-chlorocinnamylamino)ethyl] -N-(3-pyridylmethyl)-5-isoQuinolinesulfonamide.3H Pale yellow amorphous form.
IR(KBr)cm 1: 3420, 2800, 1350, 1150, 590; NMR(D20)Eppm: 3.12 (2H, brt), 3.8-4.0 (4H, m), 4.96 (2H, s), 6.0-6.2 (1H, m), 6.70 (1H, d, J = 15.7 Hz), 7.37 (4H, brq), 7.93 (lH, t, J = 6.3 Hz), 8.16 (2H, brt), 8.54 (1H, d, J = 5.8 Hz), 8.61 (1H, d, J = 6.7 Hz), 9.76 (1H, s).
Example 228. N-[ 2-(3 4-dimethoxy-&alpha;-methylcinnamyl -amino)ethyl-5-isoquinolinesulfonamide.2HCl Yellow amorphous form.
HMR(D2O)Eppm: 1.56 (3H, d, J = 6.7 Hz), 3.1-3.2 (2H, m), 3.35-3.45 (2H, m), 3.84 (3H, s), 3.91 (3H, s), 4.0-4.5 (1H, m), 6.0 (1H, dd, J = 15.6, 9.0 Hz), 6.64 (1H, d, J = 15.6 Hz), 6.92 (3H, s), 8.07 (1H, t, J = 8.0 Hz), 8.60 (1H, d, J = 8.0 Hz), 8.73 (1H, d, J = 8.0 Hz), 8.73 (1H, d, J = 6.7 Hz), 8.95 (lH, d, J = 6.7 Hz), 9.68 (1H, s).
Example 229. N-[2-(&alpha;-methyl-3, 4, 5 ,trimethoxy- cinnamylamino)ethyl]1-5-isocruinolinesulfonamide. 2HCl NMR (D20) Spam: 1.65 (3H, d, J = 6.4 Hz), 3.2- 3.5 (4H, m), 3.84 (3H, s), 3.91 (6H, s), 4.1-4.3 (lH, m), 6.13 (1H, dd, J = 14.1, 8.8 Hz), 6.70 (lH, d, J = 14.1 Hz), 6.67 (2H, s), 8.16 (1H, t, J = 8.0 Hz), 8.17 (IR, d, J = 8.0 Hz), 8.75-8.85 (2H, m), 9.02 (1H, t, J = 8.0 Hz), 8.17 (1H, d, J = 8.0 Hz), 8.75-8.85 (2H, m), 9.02 (1H, d, J = 7.0 Hz), 9.80 (1H, s).
ExamPle 230. N-{2-dimethylaminoethyl)-N-[2- (N-methyl-3 4 5-trimethoxycinnamylamino) ethyl]-5 -isonolinesulfonamide Colorless oil.
NMR(CDCL3)Eppm: 2.12 (6H, s), 2.22 (3H, s), 2.39 (2H, t, J = 6.8 Hz), 3.10 (2H, d, J = 6.6 Hz), 3.4-3.5 (4H, m), 3.84 (3H, s), 3.87 (6H, s), 6.06 (1H, dt, J = 16, 6.6 Hz), 6.40 (1H, d, J = 16 Hz), 6.61 (2H, s), 7.64 (lH, t, J = 7.4 Hz), 8.15 (1H, d, J = 7.4 Hz), 8.44 (2H, m), 8.68 (lH, d, J = 6.1 Hz), 9.32 (1H, s).
Example 231. N-(2-dimetholaminoethyl)-N- [2-(N -methyl-3 4, 5-trimethoxycinnamylamino)ethyl -S -isoquinolinesulfonamide.3HCl Yellow amorphous form.
NMR (D2O)Sppm: 3.00 (6H, s), 3.08 (3H, s), 3.80 (3H, s), 3.82 (6H, s), 3.5-4.1 (lOH, m), 6.1 (1H, m), 651 (2H, s), 6.68 (1H, d, J = 16 Hz), 8.0 (lH, t, J = 16 Hz), 8.5 (2H, m), 8.7 (2H, m), 9.55 (1H, s).
Example 232. N- [2- (4-chlorocinnamylamino)ethyl) -N- (3, 4 5-trimethoxybenzyl)-5-isoquinolinesulfon -amide.2HCl Colorless amorphous form.
IR (RBr)cm 1 = 3420, 2920, 1330, 1130, 590; NMR (DMSO-d6)appm: 2.99 (2H, brs), 3.55 (6H, s), 3.56 (3H, s), 3.68 (4H, brs), 4.47 (2H, s), 6.2 - 6.4 (lH, m), 6.76 (1H, d, J = 15.9 Hz), 7.45 (4H, s), 7.95 (lH, t, J = 7.9 Hz), 8.54 (1H, d, J = 7.6 Hz), 8.6 - 8.7 (2H, m), 8.78 (1H, d, J - 6.3 Hz), 9.48 (2H, brs), 9.73 (lH, s).
Example 233. N-cyanomethyl-N- [2-(4-methyoxycorbonyl -N-&alpha;-dimethylcinnamylamino)ethyl]-5-isoquinoline sulfonamide Colorless oil.
IR (KBr)cm -1 = 2250, 1718, 1280; NMR (CDCl3)Sppm = 1.16 (3H, d, J = 6.6 Hz), 2.23 (3H, s), 2.65-2.8 (2H, m), 3.35 (lH, m), 3.43 (2H, t, J = 5.6 Hz), 3.91 (3H, s), 4.63 (2H, s), 6.23 (1H, dd, J = 15.9, 7.1 Hz), 6.46 (1H, d, J .15.9 Hz), 7.39 (2H, d, J = 8.3 Hz), 7.73 (1H, t, J = 8.3 Hz), 7.98 (2H, d, J 8.3 Hz), 8.25 (1H, d, J - 8.3 Hz), 8.4- 8.5 (2H, m), 8.70 (1H, d, J = 6.1 Hz), 9.36 (1H, s).
Example 234. N-cyanomethyl-N-[2-(4-methoxycarbonyl -N,&alpha;-dimethylcinnamylamino)ethyl]-5-isoquinoline- sulfonamide.2HCl Colorless amouphous form IRC (KBr)cm 1 = 1718, 1280; NMR (CDCl3)#ppm: 1.14 (3H, d, J = 6.6 Hz), 2.10 (6H, s), 2,21 (3H, s), 2.37 (2H, t, J = 7.3 Hz), 2.452.65 (2H, m), 3.22 (1H, m), 3.35-3.50 (4H, m), 3.91 (3H, s), 6.21 (1H, dd, J = 1.59, 6.8 Hz), 6.42 (1H, d, J = 15.9 Hz), 8.15 (1H, t, J = 7.7 Hz), 8.7-8.85 (3H, m), 8.99 (1H, d, J = 7.0 Hz), 9.76 (1H, s).
Example 235. N-(2-dimethylaminoethyl)-N- [2- (4-methoxycarbony-N,&alpha;-dimethylcinamylamino) -ethyl]-5-isoquinolinesulfonamide Colorless oil.
IRC (KBr)cm : 1718, 1280; NMR (CDCl3)#ppm: 1.14 (3H, d, J = 6.6 Hz), 2.10 (6H, s), 2.21 (3H, s), 2.37 (2H, t, J = 7.3 Hz), 2.45 2.65 (2h, m), 3.22 (1H, m), 3.35 - 3.50 (4H, m), 3.91 (3H, s), 6.21 (1H, dd, J = 15.9, 6.8 Hz), 6.42 (1H, d, J = 15.9 Hz), 7.38 (2H, d, J = 8.3 Hz), 7.63 (1H, t, J = 7.9 Hz), 7.98 (2H, a, J = 8.3 Hz), 8.14 (1H, d, J = 7.9 Hz), 8.4 - 8.5 (2H, m), 8.67 (1H, d, J = 6.4 Hz), 9.31 (1H, s).
Example 236. N- ( 2-dimethylaminoethyl -N- [ 2-(4 methoxycarbonyl-N, &alpha;-dimethylcinnamylamino 5 ethyl 1 -5-isoquinolinesulfonamide.3HCl Colorless amorphous.
NMR (D2O) #ppm: 1.53 (3H, d, J = 6.7 Hz), 2.95 (9H, s), 3.3-3.6 (4H, m), 3.9 - 4.0 (4H, m), 4.0 (3H, s), 4.25 (1H, m), 6.30 (1H, m), 6.75 (1H, d, J = 16.0 Hz), 7.37 (2H, brs), 7.81 (2H, brs), 8.04 (1H, t, J = 8.1 Hz), 8.55 (2H, m), 8.65 (1H, d, J = 7.0 Hz), 9.60 (lH, s).
Example 237. N-(2-morpholinoethyl )-N-[2-(4- methoxv-corbonvl-N, &alpha;-dimethylcinnamylamino)ethyl] -5-isoquinolinesulfonamide Colorless oil.
IR (KBr)cm : 1720, 1280; NMR (CDC13)Eppm: 1.15 (3H, d, J = 6.4 Hz), 2.21 (3H, s), 2.25-2.7 (2H, m), 3.1-3.3 (1H, m), 3.4-3.6 (8H, m), 3.91 (3H, s), 6.20 (1H, dd, J = 16.1, 7.3 Hz), 6.42 (1H, d, J = 16.1 Hz), 7.38 (2H, d, J = 8.3 Hz), 7.63 (lH, t, J = 7.8 Hz), 8.42 (1H, d, J = 7.8 Hz), 8.42 (1H, d, J = 7.1 Hz), 8.67 (1H, d, J = 7.1 Hz), 9.32 (1H, s).
Example 238. N-(2-morpholinoethyl)-N-[ 2- (4-methoxy -carbonyl-N,&alpha;-dimethylcinnamylamino)ethyl-5- isoquino-linesulfonamide. 3HCl Colorless amorphous form NMR (D20)Eppm: 1.55 (3H, d, J = 6.8 Hz), 3.00 (3H, s), 3.2-3.7 (8H, m), 3.8-4.1 (8H, m), 4.0 (3H, s), 4;24 (1H, m), 6.35 (lH, m), 6.76 (1H, d, J = 16 Hz), 7.40 (2H, brs), 7.82 (2H, brs), 8.06 (1H, t, J = 7.5 Hz), 8.5-8.75 (3H, m), 8.80 (1H, d, J = 7.0 Hz), 9.63 (1H, 5).
Example 239. 39. ( N-(2-aminoethyl)-N- [2-(4-methoxy -carbonyl-N, &alpha;-dimethylcinnanylamino)ethyl]-5 -isoouinolinesulfonamide Colorless oil.
-1 IR (KBr)cm : 1718, 1280; NMR (CDCl3)Sppm: 1.13 (3H, d, J = 6.6 Hz), 2.19 (3H, s), 2.6 (2H, m), 2.86 (2H, brs), 3.22 (lH, m), 3.37 (4H, t, J = 6.9 Hz), 3.91 (3H, s), 6.20 (lH, dd, J = 16.0, 6.9 Hz), 6.42 (IH, d, J = 16.0 Hz), 7.39 (2H, d, J = 8.3 Hz), 8.14 (1H, d, J = 8.1 Hz), 8.38 (1H, d, J = 8.1 Hz), 8.45 (1H, d, J = 6.1 Hz), 8.68 (1H, d, J = 6.1 Hz), 9.31 (1H, s).
Example 240. N-(2-aminoethyl)-N-[2-(4-methoxycar- bonyl-N,&alpha;-dimethylcinnamylamino)ethyl]-5 isoquinolinesulfonamlde.3HCl Colorless amorphous form.
NMR(D20)Eppm: 1.53 (3H, d, J = 6.4 Hz), 2.96 (3H, s), 3.3 - 3.5 (4H, m), 3.8 - 4.0 (4H, m), 4.0 (3H, s), 4.2 (lH, m), 6.3 (lH, m), 6.76 (lH, d, J = 15.9 Hz), 7.35 (2H, d, J = 8.0 Hz), 7.78 (2H, brs), 803 (1H, t, J = 7.9 Hz), 8.6 (2H, m), 8.66 (1H, d, J = 6.7 Hz), 8.85 (1H, d, J =6.7 Hz), 9.58 (1H, s).
Example 241. N-[ 2 (4-chloro-N-methylcinnamyl- amino)ethyl]-N-methoxycarbonolmethyl-5- isoquinoline-sulfonamide.2HCl Yellow amorphous form.
IR (KBr)cm -1 3420, 2650, 1750, 1350, 1150, 840, 590; NMR (D2O)Sppm: 3.05 (3H, s), 3.51 (2H, brs), 3.61 (3H, s), 3.89 (2H, brs), 4.06 (2H, d, J = 7.3 Hz.), 4.45 (2H, s), 6.2-6.4 (lH, m), 6.85 (1H, d, J = 15.6 Hz), 7.34 (4H, brq), 8.12 (1H, t, J = 8.0 Hz), 8.6 - 8.8 (3H, m), 8.95 (lH, d, J = 7.0 Hz), 9.79 (lH, s).
Example 242. N-carboxvmethvl-N-(2-(4-chloro-N -methylcinamylamino)ethyl]-5-isoquinolinesulfor- namide Pale brown amorpous form.
IR (KBr)cm : 3420, 1620, 1330, 1140, 590: NMR(DMSO-d6)6ppm: 2.43(3H, s), 2.89 (2H, brt), 3.3-3.6 (4H, m), 3.91 (2H, s), 6.2-6.4(1H, m), 6.67 (1H, d, J = 15.8 Hz), 7.45 (4H, brq), 7.85 (1H, t, J = 8.0), 8.3-8.5 (3H, m), 8.71 (1H, d, J = 6.2 Hz), 9.49 (lH, s).
Example 243. N-T2-IN-carboxvmethvl-4-chloracinn- amylamino ) ethvl 1 -N-methyl-5-isopuinoliesulfonainide Pale yellow amorphous form.
IR (KBr)cm-1: 3400, 1630, 1320, 1140, 590; NMR (DMSO-d6)Eppm: 2.75 (2H, brt), 2.79 (3H, s), 3.2-3.4(6H, m), 6.1-6.3 (lH, m), 6.50(1H, d, J = 16.2 Hz), 7.39 (4H, brq), 7.83 (1H, t, J = 7.8 Hz), 8.3-8.5 (3H, m), 8.68 (lH, d, J = 6.1 Hz), 9.48 (1H, s).
Example 244. N-[2-(4-chloro-N-methylcinnamyl- amino)ethyl]1-N-methyl-5-isoauinolinesulfonamide .2HC1 Pale brown amorphous form.
IR (KBr)cm-l: 3420, 2670, 1350, 1140, 830, 590; NMR(D20)Eppm: 3.00 (3H, s), 3.04 (3H, s), 3.51 (2H, brs), 3.66 (2H, brs), 4.10 (2H, brd), 6.2-6.4 (1H, m), 6.90 (1H, d, J = 15.9 Hz), 7.39 (4H, brq), 8.15 (lH, t, J = 8.0 Hz), 8.6-8.8 (3H, m), 9.08 (1H, d, J =6.3 Hz), 9.80 (1H, s).
Example 245. N-carbamoyl-N- F 2- (4-chrolo-N -methylcinnamylamino) ethyl]-5-isoquinolinesulfo namide.2HCl Colorless amorphous.
IR (KBr)cm : 3420, 2670, 1680, 1350, 1150, 840, 590; NMR (D20)Eppm: 3.04 (3H, s), 3.4-3.7 (2H, m), 3.89 (2H, brt), 4.06 (2H, brt), 4.29 (2H, s), 6.2-6.4 (1H, m), 6.86 (1H, d, J = 7.0 Hz), 9.80 (lH, s).
Example 246. N-[ 2 - 2-(4-chlorocinnamylmino)ethyl]- N-[[(5-methyl-4-imidazolyl )methyll-S-isoouinoline sulfonamide.HCl Pale yellow amorphous form.
IR (KBr) cm 1 3420, 3020, 1350, 1150, 830, 590; NMR(D20) Sppm: 2.49 (3H, s), 3.52 (4H, brs), 4.10 (2H, brd), 4.70 (2H, s), 6.1- 6.3 (1H, m), 6.83 (1H, d, J = 15.8 Hz), 7.34 (4H, s), 8.10 (1H, d, J = 8.0 Hz), 8.6-8.8 (3H, m), 8.83 (1H, s), 8.95 (1H, d, J = 7.0 Hz), 9.81 (1H, s).
Example 247. N-F2-(4-chloro-N-methoxycarbonyl- methylcinnamolamino)ethyll-N-methol-5-isoQuniline- sulfonamide. 2HCl Yellow amorphous form.
IR (KBr)cm 1 3420, 2620, 1750, 1350, 1140, 840, 590; NMR(D2O)Sppm: 3.07 (3H, s), 3.73 (4H, brt), 3.89 (3H, s), 4.2 (2H, brd), 4.37 (2H, s), 6.2-6.4 (1H, m), 6.89 (1H, a, J = 16.0 Hz), 7.32 (4H, brq), 8.14 (1H, t, J = 7.9 Hz), 8.6-8.8 (3H, m), 9.04 (1H, d, J = 7.0 Hz), 9.83 (1H, s).
Example 248. N- F 2- (N-carbamoylmethyl-4- chlorocinnanvlamino)-ethyll-N-methyl-5 isoquinoline-sulfonamide. 2HCl Pale yellow amorphous form.
IR (KBr) cm 1: 3400, 1690, 1350, 1140, 830, 590; NMR (D20) Sppm: 3.05 (3H, s), 3.5-3.8 (4H, m), 4.1-4.2 (2H, m), 4.23 (2H, s), 6.2-6.4 (1H, m), 6.91 (1H, d, J = 15.9 Hz), 734 (4H, brq), 8.11 (1H, t, J = 7.0 Hz), 8.6-8.8 (3H, m), 9.00 (1H, d, J = 7.0 Hz), 9.80 (lH, s).
Example 249. N-F 2- ( 4-chloro-N-cyanomethylcinnamyl- amino ethvl -N-methyl-5-isocniinolinesulfonamide .2HC1 Pale brown amorphous form.
IR (KBr)cm 1 3420, 2570, 1350, 1140, 830, 590; NMR (DMSO-d6)Sppm: 2.80 (2H, brt) 2.89 (3H, s), 3.3-3.5 (4H, m), 3.89 (2H, s), 6.1- 6.3 (1H, m), 6.64 (1H, d, J = 15.8 Hz), 7.43 (4H, brq), 8.09 (lH, t, J = 8.0 Hz), 8.63 (lah, d, J = 7.6 Hz), 8.7-8.9 (3H, m), 9.98 (1H, s).
Example 250. N- F 2- (4-chloro-N-methylcinnamyl- amino )~ethYl I -N-morpholinocarbonvImethyl-5-isoauino- linesulfonamide Colorless oil.
IR (KBr)cm : 1660, 1330, 1130; NMR (D20)Eppm: 2.17 (3H, s), 2.57 (2H, t, J = 6.3 Hz), 3.06 (2H, d, J = 6.3 Hz), 3.39 (4H, brs), 3.5-3.7 (6H, m), 4.41 (2H, s), 6.06 (1H, dt, J = 15.9, 6.3 Hz), 6.40 (1H, d, J = 15.9 Hz), 7.27 (4H, s), 7.66 (1H, t, J = 8.0 Hz), 8.15 (1H, d. J = 8.3 Hz), 8.43 (1H, d, J = 6.4 Hz), 8.54 (lH, d, J = 8.0 Hz), 8.66 (1H, d, J = 6.4 Hz), 9.30 (1H, s).
Example 251. N-{2-[N-(2-aminoethyl)-4-chlorocinn- amylamino] ehtyl}-N-methyl-5-isomiinolinesulfonamide .3HC1 Colorless amorphous form.
IR (KBr)cm 1 3420, 2950, 1490, 1350, 1140, 590; NMR (D20)6ppm: 3.05 (3H, s) 3.5-3.8 (8H, m), 4.20 (2H, brd), 6.2-6.4 (lH, m), 6.05 (1H, d, J = 15.9 Hz), 7.37 (4H, brq), 8.16 (1H, t, J = 7.9 Hz), 8.6-8.8 (3H, m), 9.07 (1H, d, J = 7.0 Hz), 9.84 (1H, s).
Example 252. N-[2-(4-chloro-N-methylcinnamylamino ) - ethyl]-N-[2-(1-Pyperazinyl)ethyl]-5 isoquinolinesul-fonamide.4HCl Colorless amorphous form.
IR (KBr)cm 1 3420, 2660, 1460, 1350, 1150, 590; NMR (D20)Eppm: 3.04 (3H, s), 3.3-3.7 (12H, m), 3.8- 4.1 (6H, m), 6.0 - 6.2 (1H, m), 6.73 (lH, a, J = 15.9 Hz), 7.18 (4H, s), 8.11 (1H, t, J = 7.9 Hz), 8.53 (lH, d, J = 7.3 Hz), 8.6-8.8 (2H, m), 8.89 (1H, d, J = 6.9 Hz), 9.78 (1H, s).
Example 253. N- [ 2- ( 4-chloro-N-methylcinnamyl- aminossethvll-N- r 2- (4-methvl-l-Pyperazinvl!ethvl-5 -isoquinolinesulfonamide-4HCl Colorless amorphous form IR (KBr)cm : 3420, 2660, 1460, 1140, 590; NMR (D20)Eppm: 3.02 (3H, s), 3.3-3.7 (12H, m), 3.8-4.1(6R, m), 6.1-6.3 (lH, m), 6.74 (lH, d, J = 16.0 Hz), 7.19 (4H, s), 8.11 (lH, t, J = 7.9 Hz), 8.55 (1H, d, J = 7.0 Hz), 8.6-8.8 (2H, m), 8.90 (1H, d, J = 7.0 Hz), 9.79 (1H, s).
Example 254. N-[2-(3-methyoxy-&alpha;-methylcinn- amylamino)ethyl]-5-isoquinolinesulfonamide. 2HCl Pale yellow crystalls.
Melting point 115 - 1180C; -1 IR (KBr)cm : 3420, 3200-2600, 1605, 1350, 1162, 1150; NMR (DMSO-d6)Sppm: 1.40 (3H, d, J = 6.4 Hz), 2.90-3.0 (2H, m), 3.15-3.25 (2H, m), 3.78 (3H, s), 3.80-4.0 (1H, m), 6.22 (lH, dd, J = 15.9, 8.8 Hz), 6.70 (1H, d, J = 15.9 Hz), 6.85 - 7.05 (3H, m), 7.30 (lH, t, J = 7.9 Hz), 7.30 (1H, br, disappears in D2O), 8.0 (lH, d, J = 7.9 Hz), 8.04 (lH, a, J = 7.3 Hz), 8.59 (1H, d, J = 7.3 Hz), 8.68 (lH, d, J = 7.9 Hz), 8.82 (2H, s), 8.91 (lH, m, disappears in D2O), 9.60 (2H, br, disappears in D2O), 9.88 (lH, s).
Example 255. N- r 2-(4-hodroxvmethvl-a-metholcinnamol amino ) ethvl 1 -5-isopuinolinesulfonamide Colorless crystals.
IR (KBr)cm : 1620, 1326, 1160, 1139, 831, 761, 598; NMR(CDC13)Eppm: 1.07 (3H, d, J = 6,35 Hz), near 1.95 (3H, br), 2.60 (2H, t. J = 6.0 Hz), 2.96 (2H, m), 3.05 (1H, m), 4.68 (2H, s), 5.78 (lH, dd, J = 15.87, 7.82 Hz), 6.27 (lH, d, J = 8.30 Hz), 7.67 (lH, dd, J = 8.30, 7.32 Hz), 8.18 (lH, d, J = 8.30 Hz), 8.40 (lH, d, J = 6.11 Hz), 8.44 (1H, d, J = 7.32 Hz), 8.61 (lH, d, J = 6.11 Hz), 9.32 (IR, s).
Example 256. N-T2-(a-methvl-4-methvlthiocinnamyl- amino ) ethyl 1 -5-isopuinolinesulfonamide Colorless amorphous form.
IR (KBr)cm : 1618, 1493, 1324, 1160, 1138, 1094, 830, 807, 760, 598; NMR (CDC13)Eppm: 105 (3H, d, J = 6.35 Hz), 2.48 (3H, s), 2.60 (2H, m), 2.96 (2H, t, J = 6.10 Hz), 3.03 (lH, in), 5.75 (lH, dd, J = 15.87, 7.81 Hz), 6.22 (lH, d, J - 15.87 Hz), 7.18 (4H, s), 7.67 (lH, dd, J = 8.30, 7.32 Hz), 8.17 (1H, d, J = 8.30 Hz), 8.43 (lH, d, J = 6.10 Hz), 8.44 (1H, d, J = 7.32 Hz), 8.68 (lH, d, J = 6.10 Hz), 9.34 (lH, s).
Example 257. N-f 2- ( a-methvl-4-methylsulfinyl- cinnamolamino)ethyll-5-isoauinolinesulfonamide Colorless amorphous form.
IR (KBr)cm 1; 1618, 1326, 1160, 1138, 1089, 1041, 831, 762, 599; NMR(CDC13)Eppm: 1.11 (3H, d, J = 6.59 Hz), 2.0-4.0 (2H, br), 2.65 (2H, m), 2.73 (3H, s), 3.00 (2H, t, J = 5.62 Hz), 3.15 (1H, m), 5.96 (1H, dd, J = 16.11, 7.81 Hz), 6.36 (1H, d, J = 16.11 Hz), 7.42 (2H, d, J = 8.30 Hz), 7.58 (2H, d, J = 8.30 HZ), 7.68 (lH, ad, J = 8.31, 7.32 Hz), 8.10 (1H, d, J = 8.30 Hz), 7.68 (lH, dd, J = 8.31, 7.32 Hz), 8.19 (lH, d, J = 8.31 Hz), 8.44 (1H, d, J = 6.1 Hz), 8.44 (1H, d, J = 7.32 Hz), 8.67 (1H, d, J = 6.10 Hz), 9.35 (1H, s).
Example 258. N-F2-(a-methyl-4-methylsulfonyalcynn- amylamino ethyl I -S-isouinolinesulfonamide Colorless amorphous.
IR (KBr)cm 1; 1310, 1149, 1090, 960, 832, 765, 599, 542; NMR (CDCl3)Eppm: 1.09 (3H, d, J = 6.35 Hz), 2.62 (2K, m), 2.98 (2H, t, J = 5.62 Hz), 3.05 (3H, s), 3.10 (1H, m), 6.02 (1H, dd, J = 15.87, 7.57 Hz), 6.37 (lH, d, J = 15.87 Hz), 3.05 (3H, s), 3.10 (lH, m), 6.02 (lH, dd, J = 15,87, 7,57 Hz), 6.37 (lH, d, J = 15.87 Hz), 7.44 (2H, d, J = 8.30 Hz), 7.69 (lH, dd, J = 8.06, 7.57 Hz), 7.85 (2H, d, J = 8.30 Hz), 8.20 (lH, d, J = 8.06 Hz), 8.44 (lH, d, J = 6.35 Hz), 8.45 (1H, d, J = 7.57 Hz), 8.67 (1H, d, J = 6.35 Hz), 9.36 (lH, s).
Example 259. N-[2-(4-cyano-&alpha;-methylcinnamylamino) ethyl ]-5-isoquinolinesulfonamide Colorless needles.
Melting point: 62-650C; IR (KBr)cm 1; 2230, 1620, 1322, 1140, 600; NMR (CDC13)Eppm: 1.08 (3H, d, J = 6.3 Hz), 2.57-2.65 (2H, m), 2.9-3.0 (2H, m), 3.0-3.2 (lH, m), 5.98 (1H, dd, J = 15.9, 7.8 Hz), 6,33 (lH, d, J = 15.9 Hz), 7.36 (2H, d, J = 8.3 Hz), 7.58 (2H, d, J = 8.3 Hz), 7.69 (lH, t, J = 8.0 Hz), 8.20 (1H, d, J = 8.0 Hz), 8.40-8.50 (2H, m), 8.69 (1H, d, J = 6.1 Hz), 9.36 (1H, s).
Example 260. N T 2 - ( 4 - N-F 2-(4-carbamoyl-a-methvlcinnamyl- amino ! ethyl]-5-isoquinolinesulfonamide Color less needles Melting point: 66-700C; IR (KBr)cm ; 3450, 1662, 1610, 1320, 1160, 1140; NMR (CDCl3)#ppm; 1.08 (3H, d, J = 6.4 HZ), 2.61 (2H, M), 2.90-3.20 (3H, m), 5.93 (lH, dd, J = 16.1, 7.8 Hz), 6.32 (lH, d, J = 16.1 Hz), 7.32 (2H, d, J = 8.3 Hz), 7.66 (lH, t, J = 8.3 Hz), 7.74 (2H, d, J = 8.3 Hz), 8.18 (lH, d, J = 8.3 Hz), 7.74 (2H, d, J = 8.3 Hz), 8.18 (lH, d, J = 8.3 Hz), 8.40-8.50 (2H, m), 8.67 (1H, d, J = 6.1 Hz), 9.34 (lH, s).
Example 261. N-[2-(4-acetamide-&alpha;-methylcinnamyl- amino)ethyl]-5-isoquinolinesulfonamide Colorless amorphous form.
IR (KBr)cm ; 3300-2800, 1670, 1600, 1538, 1320, 1160, 1140; NMR (CDC13)Eppm; 1.03 (3H, d, J = 6.6 Hz), 2.16 (3H, s), 2.55-2.65 (2H, m), 2.90-3.10 (3H, m), 5.68 (1H, dd, J = 15.9, 8.1 Hz), 6.20 (1H, d, J = 15.9 Hz), 7.17 (2H, d, J = 8.5 Hz), 7.44 (2H, d, J = 8.5 Hz), 7.66 (1H, dd, J = 8.3, 7.3 Hz), 8.44 (lH, d, J = 7.3 Hz), 7.66 (1H, s, disappears in D2O), 8.16 (lH, d, J = 8.3. Hz), 8.44 (lH, d, J = 7.3 Hz), 8.44 (1H, d, J = 6.1 Hz), 8.61 (1H, d, J = 6.1 Hz), 9.31 (1H, s).
Example 262. N-[2-(3-nitro-3-methoxy-&alpha;-methylcinn amvlamino )ethyl 1 -5-isoquinolinesulfonamide.HCl Colorless crystals.
Melting point: 159-1630C; IR (KBr) cm 3450, 3150-2600, 1530, 1330, 116-, 1140; NMR (DMSO-d6)Sppm: 1.34 (3H, d, J = 6.6 Hz), 2.75-3.0 (2H, m), 3.02-3.20 (2H, m), 3.90 (3H, s), 3.90-4.10 (1H, m), 6.30 (1H, dd, J = 15.6, 8.6 Hz), 6.57 (1H, d, J = 15.6 Hz), 7.26 (lH, d, J = 7.8 Hz), 7.32 (1H, d, J = 7.8 Hz), 7.83 (1H, t, J = 7.8 Hz), 8.35-8.45 (3H, m), 8.52 (1H, brs, disappears in D2O), 8.7 (1H, d, J = 6.1 Hz), 9.25 (2H, brs, disappears in D2O), 9.47 (1H, s).
Example 263. N-[2-(2-methoxy-&alpha;-metholcinnamolamino) -ethvll-5-isoauinolinesulfonamide Colorless amorphous form.
IR (KBr) cm l 1 1490, 1463, 1326, 1244, 1160, 1138, 755, 599; NMR (CDCl3) Sppm: 1.05 (3H, d, J = 6.35 Hz), 2.6 (2H, m), 2.96 (2H, t, J = 5.62 Hz), 3.04 (lH, m), 3.82 (3H, s), 5.79 (1H, dd, J = 16.12, 7.94 Hz), 6.85 (1H, brd, J = 8.06 Hz), 6.90 (1H, brt, J = 7.57 HZ), 7.21 (1H, m), 7.31 (1H, dd, J = 16.12, 7.94 Hz), 6.85 (lH, brd, J = 8.06 Hz), 6.90 (1H, brt, J = 7.57 Hz), 7.21 (1H, m) 7.31 (1H, dd, J = 7.57, (1.71 Hz), 7.66 (lH, dd, J = 8.06, 7.57 Hz), 8.16 (1H, t, J = 8.06 Hz), 8.44 (2H, m), 8.67 (1H, d, J = 6.35 Hz), 9.33 (lH, s).
Example 264.
To confirm the usefulness of the above-mentioned compound of the present invention, the following experiments were carried out.
Vessel Smooth Muscle Relaxation Action (V.R. ED3).
A rabbit was killed by bleeding and the superior mesenteric artery was removed and cut to a spiral form to prepare a band-shaped specimen according to a conventional method. The specimen was suspended by loading a strain in Krebs-Henseleit solution through which an oxygen gas containing 5% carbon dioxide was bubbled. The specimen was contracted by adding potassium chloride to maintain a predetermined strain.
Thereafter, a test compound was cumulatively administrated. The relaxation activity of the test compound was expressed by ED50 (M), i.e., a concentration of the compound which relaxes the strain to 50% of the strain only in the presence of potassium chloride (as 100%) Platelet Agglutination Inhibition (P.A.; IC50) (1) Preparation of washed platelets The blood was obtained from a healthy person and mixed with one tenth volume of 0.38% sodium citrate, and the mixture was centrifuged at 700 x G for 10 minutes to obtain a platelet-rich plasma (PRP). To the PRP was added one sixth volume of ACD solution (2.2% sodium citrate, 0.8% citric acid and 2.2% glucose; freshly prepared before use) and the mixture was centrifuged at 1500 x G for 10 minutes to obtain a platelet pellet.Next, the platelet pellet was suspended in a modified HEPES-Tyrode solution (135 mM NaCl, 2.7 mM KCl, 1 mM MgC12 , 0.1 mg/ml glucose, 20 mM HEPES; pH 7.4). To this suspension was added one sixth volume of ACD solution, and the whole was further centrifuged at 1500 x G for 5 minutes to prepare a platelet pellet. The platelet pellet was then suspended in a modified HEPES Tyrode solution to obtain about 3 x 105 l in washed platelet suspension.
(2) Measurement of Platelet agglutination To 270 Fl of the washed platelet suspension was added 3 rl of a solution of a test compound dissolved in an appropriate medium in different concentration, and the mixture was pre-incubated at 370C for 2 minutes. After an addition of 30 z of 20. pg/ml collagen solution, the absorbance was measured with 4-channel agglutination analyzer (HEMA'Tracer 601; Niko Bioscience).
(3) Determination of effect of test compounds As a control, the above-mentioned procedure was carried out except that the test medium without a test compound was used, and the absorbance before addition of collagen and the maximum absorbance after addition of collagen were measured, and the difference between both absorbances was taken as 1008 agglutination.
For test compound, the absorbance before an addition of collagen and the maximum absorbance after an addition of collagen were measured, and a percent of the inhibition was determined, compared with the control. A concentration of test compound which provides 50% of the inhibition expressed as IC50.
Calmodulin-Dependent Phosphodiesterase Inhibition (1) Preparation of Calmodulin-Dependent Phosphodiesterase (Ca PDE)Ca PDE was partially purified from the brain of rat by DEAE-Sepharose column chromatography.
(2) Preparation of calmodulin Calmodulin was purified from the calf brain using a calmodulin inhibitor W-7 affinity column.
(3) Measurement of Ca2+ PDE activity A reaction mixture contained 20 1 of 500 mM Tris-HCl (pH 8.0), 20 ra of 50 mM MgC12 , 20 iÌ of 2 mM CaC12 (or 10 mM EGTA), 20 pW of 1 mg/ml bovine serum albumin, PDE, 200 mg of calmodulin, test sample and distilled water to make total volume 200 p. To the 3 mixture was added 20 pX of 4 pM [ H)-cGMP (2.5 pCi/ml), the mixture was incubated at 300C for 15 minutes, and then heated in boiling water for 3 to 5 minutes to terminate the reaction, and cooled in ice-water bath.
20 pg of 5'-nucleotidase (Snake venum) was added to the mixture, and the mixture was again incubated at .300C for 10 minutes. After addition of about 2ml of water,~the sample was applied to a cation exchange resin column (Biorad AG.AGSOW-X4 to adsorbe the [ H]-guanosine and additionally about 2 ml of washing water for the sample was added to the column. The column was washed with about.20 ml of water, and the adsorbed [ H]-guanosine was eluted with 3 ml of 3 N NR4OH, and the elute was directly received by a vial. After an addition of 10 ml of an emulsified scintillation solution (ACS-II, AMERSHAM) the radio activity was measured by a scintillation counter LS7500 (Beckmann).The enzyme activity in the presence of calmodulin was taken as 0%, and a;concentration of a test compound in pM which provides a 50% inhibition was expressed as IC50.
The results are set forth in the following Table.
Example V.R. (ED50) P.A. (IC50) Ca2+ PDE(IC50) No. (M) (NM) 2 47 3 4 12 53 5 6 7 1.6 13 8 1.8 51 9 10 6.4 37 11 59 12 13 1.2 15 14 15 43 16 17 18 19 22 20 20 1 23 21 22 1.8 23 0.25 66 24 8.3 63 25 0.55 70 26 10 27 28 29 7.6 73 30 31 24 + 7.0 Ca 2+ Example V.R. (ED50) P.A. (IC50)Ca2 PDE(1C50) No. (#M) (EM) 32 33 21 34 1.8 35 6.9 36 0.81 10.5 37 4.4 29 38 8.1 39 40 4.7 41 1.8 10 42 6.2 43 0.36 3.8 44 45 7.5 46 4 36 47 0.39 18 48 3.9 6.5 49 0.92 50 0.67 70 11 51 0.17 38 52 9.9 53 1.3 54 1.7 55 8.6 56 1.2 39 57 0.75 50 58 0.25 63 59 60 10 61 10 62 2.3 Example V.R. (ED50) P.A. (IC50) Ca PDE(IC50) No. ( M) (ill) 63 64 65 66 67 2.2 3.6 68 3.3 + 1.4 6 69 14 70 71 14 93 72 4 6.2 73 14 21 74 2.8 75 9.7 76 2.9 77 1.4 78 1.8 79 5.5 80 2.4 13 81 1.7 32 82 0.86 33 83 0.39 24 84 43 85 75 86 13 24 87 1.7 1.9 88 4.3 14 89 90 0.33 3.4 91 1.1 10 92 63 93 0.31 3.4 Example V.R. (ED50) P.A. (IC50) Ca+ PDE(IC50) No. (AM) (pom) 94 95 96 97 39 98 22 99 2.8 61 100 95 101 29 102 24 103 104 105 5.4 12 106 8.9 28 107 108 1.2 10 109 0.59 1.3 110 3.8 9.3 111 7.0 77 112 113 0.88 20 1.2 114 54 100 115 116 1.5 117 118 119 11 51 120 19 + 3.0 121 122 123 4.8 22 124 11 Example V.R. (ED50) P.A. (IC50)Ca2+ PDE(IC50) No. (pM) (#M) 125 9.2 126 10 127 39 128 1.8 129 0.82 130 13 131 1.5 132 133 134 135 136 137 138 139 140 141 142 6.8 1.1 143 0.82 1.5 144 1.8 1.0 145 12 146 2.8 147 1.2 42 148 149 150 151 152 153 1.5 25 154 60 155 4.1 55 Example V.R. (ED50) P.A. (IC50) Ca PDE(IC50) No. (AM) (AM) 156 4.4 19 19 157 3.2 36 8.6 158 17 21 159 5.1 24 92 160 1.8 56 161 4.7 162 35 163 31 164 4.1 165 11 166 1.4 90 167 1.2 52 168 3.6 26 169 4.6 170 5.2 171 5.4 172 1.0 51 9.4 173 174 11 175 2.0 13 176 1.2 55 8.5 177 2.7 56 24 178 2.4 50 179 2.8 51 7.8 180 1.8 4.8 181 8.4 38 4.7 182 17 183 2.8 184 2.8 12 185 0.21 186 Example V.R. (ED50) P.A. (IC50) Ca PDE(IC50) No. (M) ( M) 187 188 4 189-I 0.22 2.7 189-II 0.20 189-III 0.29 1.0 190 0.19 191 1.9 26 192 0.12 84 193 3.2 12 194 80 195 196 197 198 199 200 201 3.2 202 1.3 203 204 205 206 207 208 209 210 211 212 213 214 Example V.R. (ED50) P.A. (IC50) Ca2+PDE (IC50) No. ( M) ( M) 215 216 It was shown that other compounds of the present inventions described above have a platelet agglutination-inhibitory action as.well as an inhibitory action against protein kinase A, myosin light chain kinase, protein kinase C, calmodulin-dependent protein kinase II, cyclic AMP dependent phosphodiesterase and the like, but have little effect on cardiac functions.
As seen from the above-mentioned results, the present compounds as described above have a smooth muscle relaxation action, and therefore are useful as vasodilator or brain circulation-improving agents; and since the present compounds have a platelet agglutination-inhibitory action, they are useful as prophylactic or therapeutsc agents for thrombosis Moreover, since the present compounds have an inhibitory activity against various kinases, they are useful as anti-tumor agents. The above-mentioned compounds have low toxicity, and therefore are applicable as pharmaceutical preparations.

Claims (7)

1. A compound represented by the formula (I):
wherein Y represents N, H3C-N or CH; R1 represents a hydrogen atoms, an optionally substituted lower alkyl group, a formyl group, a halophenylpropargyl group, an optionally substituted aralkyl group or optionally substituted phenyl; and (1) R2 represents a group represented by the formula (II):
wherein R3 represents a hydrogen atom, an optionally substituted lower alkyl group, a formyl group, a halophenylpropargyl group, an optionally substituted aralkyl group or optionally substituted phenyl; or R1 and R3 together form a lower alkylene group; R4 represents a hydrogen atom or a lower alkyl group; R5 represents a hydrogen atom, a halogen atom, a nitro group, a lower alkyl group, an optionally substituted hydroxyl group, an optionally N-substituted amino group, an optionally substituted carboxy group, a polyfluoro-lower alkyl group, a cyano group, a hydroxymethyl group, a cyano group, a hydroxymethyl group, a methylthio group, methylsulfinyl group or methylsulfonyl group; R6 represents a hydrogen atom, halogen atom or a lower alkoxy group; or R5 and R6 together form a lower alkylenedioxy group; R7 represents a hydrogen atom or a lower alkoxy group; X represents a vinylene group or an ethynylene group; Ar represents a phenyl group, a naphthyl group or a heterocyclyl group; m represents an integer of 1 to 3; and W represents a lower alkylene group, an optionally substituted phenylene group or an optionally substituted phenylene-lower alkylene group; and quaternary ammonium salts, or salts of the compound of the formula (I).
2. A process for production of a compound according to claim 1 comprising the steps of (1) reacting a-compound represented by the formula (IV):
wherein Y, W, R1 and R3 - are as defined in claim 1, with a compound represented by the following formula (V):
wherein B represents -CH2-Hal or =CO-R4 , and other symbols have the same meanings as defined in claim 1; and optionally, (2) Reducing the compound produced in step (1), and/or optionally (3) alkylating or formylating the compound produced in step (1) or (2), and/or (4) converting the compound prepared above to a quaternary salt, or salt thereof.
3. A process for production of a compound according to claim 1 comprising the steps of: (1) reacting a compound represented by the formula (VI):
with a compound represented by the formula (VII):
or a reactive derivative thereof or a salt thereof, wherein all the symbols in formulae (VI) and -(VII) have the same meanings as defined in claim 1, and optionally, (2) alkylating the compound produced in step (1), and/or (3) converting the compound prepared above to a quaternary salt, or salt thereof.
4. Novel quinoline sulfonamino derivatives according to claim 1 and substantially as hereinbefore descibed in any Example.
5. A pharmaceutical composition comprising a compound according to claim 1 or 4.
6. A process for the production of a quinoline sulfonamino derivative according to claim 1 or 4, the process being substantially as hereinbefore described in any Example.
7. Novel intermediates for compounds according to claim l or 4, and methods for their preparation, substantially as hereinbefore described, e.g. in the Reference Examples.
GB9122595A 1988-12-26 1991-10-24 Compound having vessel smooth muscle relaxation activity Expired - Fee Related GB2248235B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005011697A3 (en) * 2003-07-24 2005-04-21 Astex Technology Ltd Sulfonamide derivatives with activity on protein kinase a and b

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0061673A1 (en) * 1981-03-20 1982-10-06 Asahi Kasei Kogyo Kabushiki Kaisha Isoquinolinesulfonyl derivatives and process for the preparation thereof
EP0187371A2 (en) * 1984-12-27 1986-07-16 Asahi Kasei Kogyo Kabushiki Kaisha Substituted isoquinolinesulfonyl compounds
EP0287696A1 (en) * 1985-11-12 1988-10-26 Asahi Kasei Kogyo Kabushiki Kaisha Isoquinoline derivatives

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0061673A1 (en) * 1981-03-20 1982-10-06 Asahi Kasei Kogyo Kabushiki Kaisha Isoquinolinesulfonyl derivatives and process for the preparation thereof
EP0187371A2 (en) * 1984-12-27 1986-07-16 Asahi Kasei Kogyo Kabushiki Kaisha Substituted isoquinolinesulfonyl compounds
EP0287696A1 (en) * 1985-11-12 1988-10-26 Asahi Kasei Kogyo Kabushiki Kaisha Isoquinoline derivatives

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005011697A3 (en) * 2003-07-24 2005-04-21 Astex Technology Ltd Sulfonamide derivatives with activity on protein kinase a and b

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