IL269671B2 - שתל קאראטופלסטי אנדותלילאלי ושיטות להכנתו - Google Patents
שתל קאראטופלסטי אנדותלילאלי ושיטות להכנתוInfo
- Publication number
- IL269671B2 IL269671B2 IL269671A IL26967119A IL269671B2 IL 269671 B2 IL269671 B2 IL 269671B2 IL 269671 A IL269671 A IL 269671A IL 26967119 A IL26967119 A IL 26967119A IL 269671 B2 IL269671 B2 IL 269671B2
- Authority
- IL
- Israel
- Prior art keywords
- graft
- collagen
- support layer
- methacrylate
- crosslinked
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 50
- 230000003511 endothelial effect Effects 0.000 title claims description 25
- 239000007943 implant Substances 0.000 title description 8
- 238000002360 preparation method Methods 0.000 title description 8
- 239000000463 material Substances 0.000 claims description 35
- 102000008186 Collagen Human genes 0.000 claims description 33
- 108010035532 Collagen Proteins 0.000 claims description 33
- 229920001436 collagen Polymers 0.000 claims description 33
- 108010010803 Gelatin Proteins 0.000 claims description 19
- 239000008273 gelatin Substances 0.000 claims description 19
- 229920000159 gelatin Polymers 0.000 claims description 19
- 235000019322 gelatine Nutrition 0.000 claims description 19
- 235000011852 gelatine desserts Nutrition 0.000 claims description 19
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 claims description 18
- 210000000399 corneal endothelial cell Anatomy 0.000 claims description 17
- 238000001035 drying Methods 0.000 claims description 16
- 239000000017 hydrogel Substances 0.000 claims description 16
- 210000004027 cell Anatomy 0.000 claims description 15
- 238000004132 cross linking Methods 0.000 claims description 13
- 238000002513 implantation Methods 0.000 claims description 11
- 210000002159 anterior chamber Anatomy 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 5
- GJKGAPPUXSSCFI-UHFFFAOYSA-N 2-Hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone Chemical compound CC(C)(O)C(=O)C1=CC=C(OCCO)C=C1 GJKGAPPUXSSCFI-UHFFFAOYSA-N 0.000 claims description 4
- 230000007613 environmental effect Effects 0.000 claims description 3
- JUYQFRXNMVWASF-UHFFFAOYSA-M lithium;phenyl-(2,4,6-trimethylbenzoyl)phosphinate Chemical compound [Li+].CC1=CC(C)=CC(C)=C1C(=O)P([O-])(=O)C1=CC=CC=C1 JUYQFRXNMVWASF-UHFFFAOYSA-M 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims description 2
- 210000000871 endothelium corneal Anatomy 0.000 claims description 2
- 210000002744 extracellular matrix Anatomy 0.000 claims description 2
- 239000010410 layer Substances 0.000 description 46
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 12
- 210000004087 cornea Anatomy 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 6
- 230000014509 gene expression Effects 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 5
- 239000004971 Cross linker Substances 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 210000002555 descemet membrane Anatomy 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 230000000155 isotopic effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000003892 spreading Methods 0.000 description 3
- 230000007480 spreading Effects 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- 201000004569 Blindness Diseases 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000002045 capillary electrochromatography Methods 0.000 description 2
- 208000015636 celiac disease-epilepsy-cerebral calcification syndrome Diseases 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 210000002889 endothelial cell Anatomy 0.000 description 2
- 210000003038 endothelium Anatomy 0.000 description 2
- 230000004438 eyesight Effects 0.000 description 2
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 1
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical group [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 1
- YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical group [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 239000012790 adhesive layer Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 210000003683 corneal stroma Anatomy 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 229910052805 deuterium Inorganic materials 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 229910052722 tritium Chemical group 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3808—Endothelial cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Cell Biology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Prostheses (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL269671A IL269671B2 (he) | 2019-09-25 | 2019-09-25 | שתל קאראטופלסטי אנדותלילאלי ושיטות להכנתו |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL269671A IL269671B2 (he) | 2019-09-25 | 2019-09-25 | שתל קאראטופלסטי אנדותלילאלי ושיטות להכנתו |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| IL269671A IL269671A (he) | 2021-03-25 |
| IL269671B1 IL269671B1 (he) | 2023-09-01 |
| IL269671B2 true IL269671B2 (he) | 2024-01-01 |
Family
ID=75267401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL269671A IL269671B2 (he) | 2019-09-25 | 2019-09-25 | שתל קאראטופלסטי אנדותלילאלי ושיטות להכנתו |
Country Status (1)
| Country | Link |
|---|---|
| IL (1) | IL269671B2 (he) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2023250450A1 (en) * | 2022-04-04 | 2024-10-17 | Precise Bio 3D Ltd | Retinal graft and method of preparation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2468312A1 (en) * | 2009-08-19 | 2012-06-27 | Tohoku University | Sheet for corneal transplants |
| EP2940216A1 (de) * | 2014-05-02 | 2015-11-04 | Küper GmbH & Co. KG | Randsteinabweiser für eine schneeräumleiste |
-
2019
- 2019-09-25 IL IL269671A patent/IL269671B2/he unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2468312A1 (en) * | 2009-08-19 | 2012-06-27 | Tohoku University | Sheet for corneal transplants |
| EP2940216A1 (de) * | 2014-05-02 | 2015-11-04 | Küper GmbH & Co. KG | Randsteinabweiser für eine schneeräumleiste |
Also Published As
| Publication number | Publication date |
|---|---|
| IL269671A (he) | 2021-03-25 |
| IL269671B1 (he) | 2023-09-01 |
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