JP2515391B2 - Automatic immunoassay device - Google Patents

Description

TECHNICAL FIELD The present invention relates to an automatic immunoassay device for automatically measuring components contained in a sample such as serum or urine by an immune reaction using a solid phase reagent.

[Conventional technology]

Enzyme-linked immunosorbent assay is a method of knowing the degree of antigen-antibody reaction using enzyme activity as a marker, and quantifying the amount of the antigen or antibody from this, by reacting the solid phase antibody (or antigen) with the antigen (or antibody), Furthermore, a sandwich method in which an enzyme-labeled antibody (or antigen) is reacted, a competitive reaction method in which the amount of the unlabeled antigen is obtained by competing the enzyme-labeled antigen (or antibody) with the unlabeled antigen (or antibody), and various other methods There is a way.

FIG. 4 is a diagram for explaining the principle of measurement by the sandwich method, and FIG. 5 is a diagram for explaining the principle of measurement by the competitive method. 61 is a solid phase carrier, 62 is an antibody, 63 is an antigen to be measured, 64 is a labeled substance, 65 is a labeled antibody, 66 is a substrate, and 67 is a product.

In the sandwich method, as shown in FIG. 4, first, an antibody 62 is immobilized on the surface of a solid phase carrier 61 by physical adsorption or a bond utilizing a chemical reaction, and the antigen 62 to be measured is included in the solid phase carrier 61. Add sample.

As a result, an antigen-antibody reaction (first reaction) occurs, and the measured antigen 63 binds to the solid-phased antibody 62, and thereafter, washing is performed to discard the miscellaneous components in the sample.

Next, the enzyme-labeled antibody 65 to which the enzyme is bound as the labeling substance 64 is added.

As a result, an antigen-antibody reaction (second reaction) occurs, and the enzyme-labeled antibody 65 is deposited on the antigen 63 bound to the immobilized antibody 62.
Combine. In this case, since the enzyme-labeled antibody 65 is added in excess, the free-form portion (fre) that is not bound to the binding-type portion (bound, B) generated by the binding of the antigen 63 and the antibody 65.
e, F) can be done.

Therefore, by washing the free part (fr
Discard excess enzyme labeled antibody of ee, F). In other words, B / F
Perform separation.

Next, an enzymatic reaction is performed. Since the enzyme activity at this time is determined by the amount of the enzyme-labeled antibody in the binding type portion, the amount of the enzyme-labeled antibody represents the amount of antigen bound to the immobilized antibody, that is, the amount of antigen to be measured.

In the competitive reaction method, as shown in FIG. 5, the antibody is immobilized and the antigen to be measured and the labeled antigen in which the label is bound to the same antigen as the antigen to be measured are added.

As a result, an antigen-antibody reaction occurs, and the antigen to be measured and the labeled antigen bind to the solid-phased antibody in proportion to their respective amounts.

Then, B / F separation is performed in the same manner as the sandwich method, and the enzymatic reaction is performed. Since the enzyme activity at this time is determined by the amount of the enzyme-labeled antibody in the bound portion, the amount of the antigen to be measured can be determined from the amount of the labeled antigen added by using a calibration curve.

All of the above methods are so-called separation methods, and there is also a non-separation method in which B / F separation is not performed. The non-separation method has a short measurement time and is easy to operate, but has a drawback such as low measurement sensitivity. On the other hand, the separation method has a high measurement sensitivity but is very complicated and troublesome to operate, and it is difficult to automate it.

When automating the above-mentioned enzyme immunoassay, a solid phase carrier such as polystyrene balls or glass beads on which an antibody or an antigen is immobilized is used as a solid phase reagent. Since this solid-phase reagent is unstable, it is usually placed in a container filled with a preservative solution, and when performing enzyme immunoassay, the solid-phase reagents are transferred from the container to the reaction detection container one by one. , Sample dispensing, labeling reagent dispensing, B / F separation, washing, etc. are performed, and then the binding type portion is transferred to the detector.

[Problems to be Solved by the Invention]

However, in the conventional automated enzyme immunoassay,
As described above, the solid-phase reagents are transferred one by one from the container to the reaction detection container, and after the reaction, they are transferred to the detector, so that the solid-phase reagent may be taken in and out. In addition, B /
Even in F separation, the free portion is separated from the solid-phase reagent, removed, and further washed, but in this case, the conventional reaction detection container cannot be processed in a stationary state, and the free To drain the portion, it is necessary to perform an operation such as tilting the reaction detection container. Moreover, when cleaning
It is difficult to cleanly drain the wash water while leaving the solid phase reagent. The same applies to washing after taking out the solid phase reagent.

As described above, in the conventional automation device, there are problems that operations for loading and unloading the solid phase reagent, B / F separation, and washing are complicated, and the mechanism is complicated. As a result, an extra processing time is required and a dead time is generated, so that the operating efficiency of the device is reduced. Furthermore, immobilized solid phase beads, etc.
In order to take it in and out of the reaction detection container, a large thing of about several mmφ is required, and the contact area is reduced in the immune reaction. Furthermore, since the change in absorbance is measured by enzyme reaction measurement, there is a problem that the measurement sensitivity is not good.

The present invention solves the above-mentioned problems, and an object of the present invention is to provide an automatic immunoassay device that can perform immunoassay automatically and with high sensitivity.

[Means for solving the problem]

Therefore, the present invention is an automatic immunoassay device for quantitatively measuring the amount of a sample antigen or antibody by an immunoreaction with a sample detection antigen or antibody by setting a reaction detection container in a reaction turntable having a plurality of positions, A solid phase reagent having a filter and a thin discharge hole at the bottom and closing the discharge port and the upper opening, and using fine-diameter granules as a solid phase carrier on the surface of the solid phase carrier with an antibody or antigen immobilized thereon, together with a preservative solution Enclosed reaction detection container and sample cup, storage turntable for storing reagent bottle, detector for detecting the amount of labeled reagent in reaction detection container, and attachment / detachment of reaction detection container to / from reaction turntable and dispensing of sample and reagent The reaction detection container is equipped with an arm mechanism to perform the reaction detection by feeding the reaction turntable forward one position at a time and penetrating the discharge hole. A mechanism is set on the reaction turntable and pressurized air is supplied from the upper opening to remove the preservation solution from the discharge hole, dispense the sample and dispense the labeling reagent, and accelerate the immune reaction for a certain period of time. It is characterized in that the reaction detection container after / F separation is transferred to a detector. Further, the sample dispensing and the labeling reagent dispensing are performed at the same position, two types of rotation pitches are set, and the two types of rotation pitches are alternately applied to rotate the reaction turntable, and the reaction turntable is rotated in the forward direction. Characterized by sending position by position.

[Action]

In the automatic immunoassay device of the present invention, a reaction detection container having a discharge hole at the bottom and containing a solid-phase reagent in which an antibody or an antigen is immobilized on the surface of the solid-phase carrier is used as a solid-phase carrier. The sample is dispensed while the turntable is moved forward one position at a time, the labeling reagent is dispensed, the immune reaction is accelerated for a certain period of time, and the reaction detection container is transferred to the detector after B / F separation. By making the reaction detection container disposable and draining and exhausting it from the discharge hole at the bottom, the processing up to transfer to the detector can be performed in a stationary state without tilting the reaction detection container.

Further, when the sample dispensing and the labeling reagent dispensing are performed at the same position, two types of rotation pitches are set, and the two types of rotation pitches are alternately applied to rotate the reaction turntable, and the forward direction is set. Immune reaction can be measured by the sandwich method while sending each position to
By allowing the labeling reagent to be dispensed at the same time as the sample is dispensed, the immune reaction can be measured by the competitive reaction method.

〔Example〕

 Hereinafter, embodiments will be described with reference to the drawings.

FIG. 1 is a layout diagram showing an embodiment of an automatic immunoassay device according to the present invention, and FIG. 2 is a diagram showing an embodiment of a cartridge used in the automatic immunoassay device according to the present invention. In the figure, 1 is a reaction turntable, 2 is a cartridge turntable, 3 is a reagent turntable, 4 is a sample turntable, 5 is a sample cup, 6 is a disposable chip, 7 to 9 are arm mechanisms, 10 is a detector, 11 Is dust, 12 is a control processing unit, 21 is a cartridge body, 22 is a filter, 23 is a solid-phase reagent, 24 is a discharge hole, 25 is an opening, 26 is an aluminum cap, 27 is an orient, and 28 is a tip.

In FIG. 1, the cartridge turntable 2 is
It is a table that stores and rotates a disposable cartridge (reaction detection container) as shown in Fig. 2, and is an example in which it is composed of 10 removable cassettes and each cartridge can store 30 cartridges. Is shown. According to this, since the same individualized antibody cartridge is stored in each section, it is possible to prepare a cartridge for 10 items. The reagent turntable 3 is a table that stores and rotates a reagent antibody reagent bottle and a disposable chip for dispensing the labeled antibody, and shows an example in which 10 types of reagent bottles, that is, 10 reagent bottles can be stored. There is. The sample turntable 4 is a table that stores and rotates a sample cup 5 that stores a sample and a disposable chip 6 that dispenses the sample. Two sample disposable chips 6 are provided inside the sample turntable 4 corresponding to each sample cup 5. An example of enabling storage is shown. The reaction turntable 1 is for carrying out sample dispensing, addition of labeled antibody, stirring reaction by vibration, washing, etc. as described above while the cartridge of the cartridge turntable 2 is set and rotated one position at a time. . Arm mechanism 7
Numerals 9 to 9 are mechanisms for inserting / removing a cartridge and dispensing a reagent or a sample between the reaction turntable 1, the cartridge turntable 2, the reagent turntable 3 and the sample turntable 4, respectively. The circles a, b, and c are shown. Further, the detector 10 is for injecting a luminescent reagent into the cartridge after the reaction to detect the luminescence amount, and the dust 11 is about to discard the cartridge after the luminescence amount is detected.

As shown in FIG. 2, the cartridge used in the automatic immunoassay device according to the present invention has a cartridge body 21 having a cylindrical shape, a solid phase reagent 23 placed therein, and a filter 22 provided below the solid phase reagent 23. Further, a thin discharge hole 24 is provided partway below the filter 22, and an opening 25 at the upper end is closed by an aluminum cap 26. Solid phase reagent 23 is several tens of μm
The antibody is immobilized on the surface of granules of about φ, and the antibody and antigen of the solid-phase reagent 23 are proteins that are easily decomposed, and thus are stored as a preservative or a buffer solution for maintaining a constant pH. It is submerged in liquid.

In addition, there is a cutout 27 in the portion where the discharge hole 24 is provided, and the tip 28 can be bent to easily remove from the cartridge main body 21 to penetrate the discharge hole 24. be able to. The discharge hole 24 is formed with an extremely small diameter, and the filter 22
Therefore, when the cartridge is used, even if the tip portion 28 is removed from the cartridge body 21, the dispensed sample, reagent, washing water, etc. are not easily discharged from the discharge hole 24, The compressed air is supplied from the opening 25 at the upper end or sucked from the discharge hole 24 to be discharged.

Therefore, the cartridge has an aluminum cap on the top.
The solid-phase reagent 23 is stored on the filter 22 in the state where the lower end is completely sealed by the tip portion 28 by the 26 and is stored in the cartridge turntable 2. When the cartridge is transferred from the cartridge turntable 2 to the reaction turntable 1 by the arm mechanism 7,
The tip portion 28 of the dust 11 is removed, and the preservative solution is discharged and washed at the next position of the reaction turntable 1.

Next, the operation will be described based on the flow system of the automatic immunoassay device according to the present invention.

Fig. 3 is an overall flow diagram, 31 is a drain tank,
32 is a compressor, 33 is an in-out switching valve,
34-37, 51 and 53 are pumps, 38-41 are tanks, 42 is 3-way joint, 43 is resistance tube, 44 is preheater, 45, 52 and 54
Is a valve and 46 is a mixer.

As shown in FIG. 3, the flow system is a reaction turntable 1 having 29 positions, with the position serving as a starting point, to which a flow system for dispensing and washing samples and reagents is connected. At the position of the base point, the cartridge is first set on the reaction turntable, and the reaction turntable is alternately rotated by two predetermined positions.
The cartridge after the reaction is transferred to the detector 10.

First, each flow system connected to the reaction turntable 1 in FIG. 3 will be described.

The drain tank 31 is for accommodating the storage liquid and the cleaning liquid discarded from each cartridge of the reaction turntable 1, and is connected to a drain passage provided in an annular shape below each position of the reaction turntable 1. The compressor 32 supplies pressurized air for discarding the preservation solution, cleaning immediately after that, cleaning in B / F separation, discarding the sample and reagent remaining at the tip of the disposable chip, and cleaning.

The tank 38 is for accommodating the luminescence auxiliary reagent, the tanks 39 and 40 are for accommodating the luminescence reagent, respectively, and the in-out switching valve 33 and the pumps 34 to 37 are for supplying the luminescence auxiliary reagent, the diluent and the luminescence reagent. It is a thing. The buffer solution stored in the tank 41 is used as the diluting solution and the cleaning solution. As this buffer solution, a mixed solution of a surfactant, sugar or the like which promotes an immune reaction is used. The tank 41 has a closed structure and is configured to supply pressurized air from the compressor 32 and apply pressure to deliver the liquid.
By controlling the cleaning liquid through the resistance tube 43, the preheater 44, and the valve 45 so as to have a stable predetermined temperature and flow rate, the reaction is facilitated and the reaction is stabilized. Similarly, in the case of the luminescent reagent, a heater may be added to the mixer 46 to stabilize the temperature during the luminescent reaction. When injecting the luminescent reagent, the mixed material remains in the tube ahead of the three-way joint 42, so it is necessary to expel it immediately before.

For example, in the pump suction step in which the in-out switching valve 33 is in the state shown in the drawing, the pump 34 sucks the luminescence auxiliary reagent from the tank 38, and the pump 35 sucks the diluent (water).
Is sucked from the tank 41, and similarly, the luminescent reagent is sucked from the tanks 39 and 40 simultaneously by the pumps 36 and 37. And
When the in-out switching valve 33 is switched (the upper half is shifted to the right) and the pump discharge process is started, the luminescence auxiliary reagent and the diluent are heated to a predetermined temperature through the preheater 44 and injected into the cartridges at the respective positions. It The luminescent reagent is a three-way joint 42, a mixer.
It is mixed through 46 and injected into the cartridge of the detector 10.

In the cleaning process, the valve 45 is selectively opened and closed, and the buffer solution is injected from the tank 41 into the cartridge at each position through the resistance tube 43, the preheater 44, and the valve 45. Then, next, the air valve is selectively opened and closed, and the compressed air is supplied from the compressor 32 to the cartridge at each position, through the air valve. In normal cleaning, injection of a cleaning solution (buffer solution) and discarding with pressurized air are performed four times.

At the position, a sample dispensing flow system and a labeled antibody reagent dispensing flow system are connected so that the sandwich method and the competitive reaction method can be applied. Is used for inhalation and dispensing. In this case, since the sample or reagent remains at the tip, a flow system of pressurized air is connected so as to blow them out by pressurized air. that is,
In the sample dispensing system, it is a flow system of a valve 52. The tip of the disposable chip is inserted into the sample cup of the sample turntable 4, the sample is sucked by the sampling pump 51, and the sample is dispensed to the cartridge at the position. Can be switched to. Similarly, when a reagent is dispensed, the tip of the disposable chip is inserted into the reagent bottle of the reagent turntable 3, sucked and dispensed by the reagent pump 53, and then the valve 54 is connected to the pressurized air flow system. Can be switched. Further, since the suction amount becomes unstable when the internal pressure rises, a valve for opening to the atmosphere is also provided.

Next, the rotation of the position of the reaction turntable 1 will be described.

The arm mechanism 7 operates at the position to convey a new cartridge from the cartridge turntable 2 and remove the leading end of the cartridge to set it.

When the new cartridge is set in the position, even if the tip 28 shown in FIG. 2 is removed from the cartridge, the preservative solution in the cartridge is not discarded, so pressurized air is sent from the upper opening of the cartridge at the position. Discard the stock solution inside.

At the next position, the washing valve is set in the upper opening of the cartridge, and the washing liquid and the pressurized air are alternated, for example, 4
Wash by repeatedly sending.

Subsequently, at the position, the diluent is added. this is,
When blood, serum, urine, etc. are directly injected, various components may interfere with the immune reaction, which makes the immune reaction easy to occur.

Then, at the position, the sample is sucked from the sampling cup with the disposable chip and dispensed into the cartridge.

After that, each time it rotates one position at a time, vibration is applied to stir it to accelerate the immune reaction (first reaction),
The B / F separation described above is performed by repeatedly performing water washing and draining with pressurized air four times at the position.

Here is a detailed description of the operation after B / F separation,
First, rotate 20 positions forward from the position, stop at the position once, inject the diluent (buffer solution) as a buffer, then rotate 10 positions forward and advance to the position 4 positions earlier than the previous time.
Here, after stirring by vibration, similarly, the sample is rotated in the 20-position forward direction and once stopped at the position to dispense the labeled antibody. The diluting solution has a function of preheating to stabilize the reaction temperature, smoothing and promoting the immune reaction, and enhancing the stirring effect when the following labeling reagent is dispensed.
This is the operation in the case of the sandwich method.

In other words, with the operation of rotating the pitch of 10 positions
By alternating the operation of rotating the pitch of 20 positions, you can move forward one position from the previous position. Since this is done, the sandwich method can also adopt an apparatus configuration in which the labeling reagent is dispensed at the sample dispensing position. As a result, the immunoassay can be performed by the same flow system even in the competitive reaction method by controlling the rotation operation so that the sample and the labeled antigen are dispensed simultaneously at the position.

After that, similarly to the first reaction until the position, the immune reaction (second reaction) is accelerated by agitating while agitating while alternately rotating by the pitch of 10 position and the pitch of 20 position, and the B / Perform F separation.

Then, a luminescence assisting reagent for enhancing luminescence at the position is added, and the cartridge is transferred to the detector 10 at the first position. The detector 10 measures the amount of luminescence immediately after adding the luminescent reagent to the cartridge. The luminescent reagent varies depending on the labeling substance. For example, in the case of Acridinium, a mixed solution of hydrogen peroxide and alkali, luminol (Lu
In the case of the (minol), a mixed solution of hydrogen peroxide and Fe ions is used, but since these react in a short time, they are simultaneously injected from the respective bottles through the three-way joint 42 and the mixer 46.

In the above operation, for example, after staying at the position for 12 seconds, rotating in the forward direction at a pitch of 20 positions, resting at the position for 12 seconds, then rotating to the position at a pitch of 10 positions, and then taking a position for 24 seconds. From position to ..., one position at a time, the cartridge is first transferred to the detector 10 in 12 seconds, a new cartridge is brought from the cartridge turntable 2 and set. In this case, it takes about 3 minutes for the first reaction and about 5 for the second reaction.
It takes a minute, and an immune reaction measurement of one sample can be performed in about 10 minutes as a whole, and a measurement speed of about 150 tests / hr can be realized.

In addition, when measuring multiple items with one sample,
At the position, the solid phase reagent cartridges corresponding to the respective measurement items are set on the cartridge turntable, the same sample is dispensed to these cartridges, and the reagents corresponding to the measurement items are further dispensed. Then, the light emission amount is measured by the detector 10, and the process ends. If measurement is required again, use the remaining disposable tip to dispense the sample. Therefore, in the sample turntable of FIG. 1, two disposable chips are stored for each sample. The re-measurement is not limited to the case where the measured value shows an abnormal value that deviates significantly from the preset range, and the judgment value is given in advance for each measurement item as the primary screening, and the next measurement item is determined from the judgment result. There are cases where it is set. For example, in a serum hepatitis test, first, an HBS antigen test is performed, and if a positive result is obtained, an HBE or HBS antigen test is performed. Further, the reason why the disposable disposable chip is used is that the measurement range is very wide in the case of immunoassay and the conventional pipette cannot completely wash it.

The present invention is not limited to the above embodiment, and various modifications can be made. For example, in the above-mentioned embodiment, the operation of the reaction turntable is such that it is alternately rotated in the forward direction by the pitch of 10 positions and the pitch of 20 positions. It may be rotated in the opposite direction depending on the pitch and stopped at the same position. The pitch to be rotated depends on the number of positions of the reaction turntable, the operation interval (12 seconds in each of the above embodiments), the required reaction time, and the like. In short, according to the present invention, when two kinds of rotation pitches are set, two kinds of rotation pitches are alternately applied to rotate the reaction turntable, and the reaction turntable is rotated once at two kinds of rotation pitches. It is needless to say that it is possible to send one position at a time in the forward direction, and it is possible to appropriately change within the range where such a condition is satisfied. Therefore,
For example, in the above embodiment, the reaction turntable is rotated by the pitch of 10 positions and the pitch of 20 positions, but it can be changed to the pitch of 11 positions and the pitch of 19 positions (in the opposite direction, the pitch of 10 positions). Of course, you can also do it.

Further, although the automatic luminescence immunoassay by the sandwich method has been described, the automatic luminescence immunoassay by the competitive reaction method is also possible by the device configuration shown in FIGS. 1 to 3, and other immunoassay methods than luminescence immunoassay, for example, Of course, it can be applied to enzyme immunoassay. Furthermore, the two types of luminescent reagents were mixed and injected into the cartridge transferred to the detector. Since such luminescent reagents depend on the labeling substance of the antibody, depending on the labeling substance of the antibody, It differs as appropriate. Further, the cleaning liquid and the supply of the pressurized air are alternately repeated four times, but it goes without saying that the number of times can be appropriately changed.

〔The invention's effect〕

As is clear from the above description, according to the present invention, since the opening at the upper end, the disposable cartridge having the discharge hole at the lower end is used as the reaction detection container, the dispensing of the sample or the reagent from the opening at the upper end, It is possible to easily wash and dispose of these by supplying pressurized air.
The device configuration can be simplified. Moreover, since the cartridge is disposable, the washing process can be reduced, and the measurement speed can be improved. In addition, since two types of rotation pitches are set and the reaction turntable is stopped halfway and rotated one position at a time, the competitive reaction method and the sandwich method can be adopted in the same device configuration by selecting the number of positions of the rotation pitch. it can. Furthermore, since a cartridge containing a solid phase reagent in which an antibody or an antigen is bound to a granule having a diameter of a few tens of μm is used as a reaction detection container, it can be used as a solid phase reagent using large beads or the like like a conventional device. In contrast, the contact area is large, and the antigen-antibody reaction time is fast, which enables short-time measurement. Moreover, since a luminescence auxiliary reagent or a luminescence reagent is added to perform luminescence immunoassay,
A highly sensitive immunoassay can be performed.

[Brief description of drawings]

FIG. 1 is a layout diagram showing an embodiment of an automatic immunoassay device according to the present invention, FIG. 2 is a diagram showing an embodiment of a cartridge used in the automatic immunoassay device according to the present invention, and FIG.
FIG. 4 is a diagram for explaining the principle of measurement by the sandwich method, and FIG. 5 is a diagram for explaining the principle of measurement by the competitive method. 1 ... Reaction turntable, 2 ... Cartridge turntable, 3 ... Reagent turntable, 4 ... Sample turntable, 5 ... Sample cup, 6 ... Disposable tip, 7-9 ... Arm mechanism, 10 ... … Detector, 11…
… Dust, 12 …… Control processing part, 21 …… Cartridge body, 22 …… Filter, 23 …… Solid phase reagent, 24 …… Discharge hole, 25 …… Opening part, 26 …… Aluminum cap, 27 …… Orime , 28 …… Tip.

Front Page Continuation (72) Inventor Yoichi Inaba 3-1-2 Musashino, Akishima-shi, Tokyo Nihon Electronics Co., Ltd. (56) Reference JP-A-60-115865 (JP, A) JP-A-62-502706 (JP, A)

Claims (3)

(57) [Claims] 1. An automatic immunoassay device for quantitatively measuring the amount of an antigen or antibody in a sample by setting a reaction detection container on a reaction turntable having a plurality of positions and performing an immunoreaction with the antigen or antibody in the sample. It has a filter and a thin discharge hole, and closes the discharge port and the upper opening, and uses a small-diameter granule as a solid phase carrier on the filter to enclose a solid phase reagent in which an antibody or antigen is immobilized on the surface of the solid phase carrier together with a preservative solution. Reaction detection container, sample cup, storage turntable for storing reagent bottle, detector for detecting the amount of labeled reagent in reaction detection container, and attachment / detachment of reaction detection container to / from reaction turntable and dispensing of sample and reagent Equipped with an arm mechanism for performing, the reaction detection container with the discharge hole penetrating the arm while moving the reaction turntable forward one position at a time The reaction solution is set on the reaction turntable and pressurized air is supplied from the upper opening to remove the preservation solution from the discharge hole, dispense the sample, dispense the labeling reagent, and accelerate the immune reaction for a certain period of time. An automatic immunoassay device characterized in that the reaction detection container after F separation is transferred to a detector. 2. The automatic immunoassay device according to claim 1, wherein the sample dispensing and the labeling reagent are dispensed at the same position. 3. An automatic system according to claim 1, wherein two kinds of rotation pitches are set, and the two kinds of rotation pitches are alternately applied to rotate the reaction turntable, and the reaction turntable is forwarded one position at a time. Immunoassay device.