JP2525638B2 - Milk fat percentage enhancer - Google Patents
Milk fat percentage enhancerInfo
- Publication number
- JP2525638B2 JP2525638B2 JP63051607A JP5160788A JP2525638B2 JP 2525638 B2 JP2525638 B2 JP 2525638B2 JP 63051607 A JP63051607 A JP 63051607A JP 5160788 A JP5160788 A JP 5160788A JP 2525638 B2 JP2525638 B2 JP 2525638B2
- Authority
- JP
- Japan
- Prior art keywords
- fat percentage
- milk fat
- lactose
- milk
- enhancer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Description
【発明の詳細な説明】 産業上の利用分野 本発明は、乳牛に経口投与すると牛乳脂肪率を上昇さ
せる効果を示す乳牛の乳脂肪率の上昇剤に関する。TECHNICAL FIELD The present invention relates to an agent for increasing the milk fat percentage in dairy cows, which shows the effect of increasing the milk fat percentage when orally administered to dairy cows.
従来の技術 近年、乳業メーカーは酪農家からの牛乳の買入れに際
して、牛乳の品質に関して一定の規準を定めて、その規
準に合格しない牛乳の買入を中止している。牛乳の脂肪
率に関しては、各都道府県で規準値は異なるが、乳業メ
ーカーはその規準値に達しない牛乳を生産した酪農家に
対してペナルテイーを課している。2. Description of the Related Art In recent years, dairy manufacturers have set certain standards for milk quality when purchasing milk from dairy farmers, and have stopped purchasing milk that does not meet the standards. Regarding the fat percentage of milk, each prefecture has different standard values, but dairy manufacturers penalize dairy farmers who produce milk that does not reach the standard value.
したがつて、牛乳脂肪率を上昇させる添加剤の開発が
強く望まれている。Therefore, the development of an additive that increases the milk fat percentage is strongly desired.
発明が解決しようとする課題 本発明者らは、ガラクトオリゴ糖類を家畜飼料へ利用
する目的で、家畜に対する活性を検討している過程にお
いて、ガラクトオリゴ糖類を乳牛に経口摂取させると、
牛乳脂肪率が上昇することを見出した。DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention In the process of examining the activity against livestock for the purpose of utilizing galacto-oligosaccharides for livestock feed, the present inventors allow dairy cows to orally ingest galacto-oligosaccharides,
It has been found that the milk fat percentage increases.
すなわち、本発明は、上記知見に基いてなされたもの
であつて、ガラクトオリゴ糖類を有効成分とする乳牛の
乳脂肪率の上昇剤を提供することを目的とする。That is, the present invention has been made based on the above findings, and an object thereof is to provide an agent for increasing the milk fat percentage of dairy cows, which contains a galacto-oligosaccharide as an active ingredient.
以下本発明を詳しく説明する。 The present invention will be described in detail below.
発明の構成 本発明の特徴は、ガラクトオリゴ糖類を有効成分とす
る乳牛の乳脂肪率の上昇剤にある。Structure of the Invention The feature of the present invention is an agent for increasing the milk fat percentage of dairy cows, which contains a galactooligosaccharide as an active ingredient.
ここで用いる“ガラクトオリゴ糖”とは、乳糖にガラ
クトースが１〜４分子結合した、それぞれのガラクトシ
ルラクトース、ジガラクトシルラクトース、トリガラク
トシルラクトースおよびテトラガラクトシルラクトース
を意味する。As used herein, the term "galacto-oligosaccharide" means galactosyl lactose, digalactosyl lactose, trigger lactosyl lactose, and tetragalactosyl lactose, which are obtained by binding 1 to 4 molecules of galactose to lactose.
課題を解決するための手段 本発明において、活性成分として用いるガラクトオリ
ゴ糖類は、下記の方法により調製し得る。Means for Solving the Problems In the present invention, the galactooligosaccharide used as an active ingredient can be prepared by the following method.
乳糖または乳糖含有物（例えば、全乳、脱脂乳、チー
ズホエー等）にβ−ガラクトシダーゼを作用らせてガラ
クトース転移反応を行わせることにより、ガラクトオリ
ゴ糖類混合物が生成する。A lactose or a lactose-containing substance (for example, whole milk, skim milk, cheese whey, etc.) is reacted with β-galactosidase to cause a galactose transfer reaction, whereby a galactooligosaccharide mixture is produced.
上記乳糖または乳糖含有物にβ−ガラクトシダーゼを
作用させるには、出発原料物質の乳糖濃度を５〜50％に
したものを基質とし、これにpH2〜８、酵素濃度0.1〜20
0単位/mlで10〜60℃の温度下で酵素を作用させるのが適
当である。なお、上記酵素を作用させるための反応時間
はオリゴ糖の収量に大きな影響を及ぼすので、最適反応
時間は実験により確認することが必要である。すなわち
上記酵素の作用により生成する反応物中のガラクトオリ
ゴ糖類を高速液体クロマトグラフイーで定量しながら、
反応時間を調整する。In order to allow β-galactosidase to act on the above-mentioned lactose or a substance containing lactose, a starting material having a lactose concentration of 5 to 50% is used as a substrate, and pH 2 to 8 and enzyme concentration of 0.1 to 20 are used as the substrate.
It is suitable to act the enzyme at a temperature of 10 to 60 ° C at 0 unit / ml. Since the reaction time for allowing the above enzyme to act has a great influence on the yield of oligosaccharides, it is necessary to confirm the optimum reaction time by experiments. That is, while quantifying the galactooligosaccharide in the reaction product produced by the action of the enzyme by high performance liquid chromatography,
Adjust the reaction time.
上記の酵素反応は、反応液を90℃以上で２〜30秒間加
熱することにより停止させることができる。酵素処理し
た反応液を、噴霧乾燥あるいはドラム乾燥すると、本発
明によるガラクトオリゴ糖類を20〜50重量％含有する粉
末が得られる。The above enzymatic reaction can be stopped by heating the reaction solution at 90 ° C. or higher for 2 to 30 seconds. The enzyme-treated reaction liquid is spray-dried or drum-dried to obtain a powder containing 20 to 50% by weight of the galactooligosaccharide according to the present invention.
本発明では、このようにして得られるガラクトオリゴ
糖類含有粉末を、飼料に対して一定量配合したものを乳
牛に給与する。In the present invention, the galactooligosaccharide-containing powder thus obtained is mixed in a certain amount with the feed, and the resulting mixture is fed to the cow.
以下に実施例を示して本発明およびその効果を具体的
に説明する。The present invention and its effects are specifically described below with reference to examples.
実施例 ガラクトオリゴ糖類の調製： 40kgのチーズホエー粉に温水60kgを加えて溶解後、ク
エン酸を加えてpHを5.0に調整したものに、β−ガラク
トシダーゼ60万単位を加えて40℃で10時間反応させた。
得られた反応液を105℃で２秒間加熱して酵素を失活さ
せた後、噴霧乾燥してガラクトオリゴ糖類を25重量％含
有する粉末を調製した。Example Preparation of galacto-oligosaccharide: After adding 60 kg of warm water to 40 kg of cheese whey powder to dissolve it, pH was adjusted to 5.0 by adding citric acid, and β-galactosidase (600,000 units) was added and reacted at 40 ° C for 10 hours. Let
The obtained reaction solution was heated at 105 ° C. for 2 seconds to inactivate the enzyme, and then spray-dried to prepare a powder containing 25% by weight of galactooligosaccharide.
乳牛の飼育試験： 分娩後３〜４ヶ月経過したホルスタイン種雌牛10頭
を、ＡおよびＢ群に分けた。各群毎に、上記の方法で調
製したガラクトオリゴ糖含有粉末を1.86重量％添加した
飼料と、対照としてガラクトオリゴ糖含有粉末の代りに
ホエー粉を添加した飼料を、下記に示した２重反転法で
給与した。Breeding test of dairy cows: Ten Holstein cows 3 to 4 months after calving were divided into groups A and B. For each group, the feed containing the galactooligosaccharide-containing powder prepared by the above method in an amount of 1.86% by weight and the feed containing whey powder in place of the galactooligosaccharide-containing powder as a control were subjected to the double inversion method shown below. I was paid.
なお、ガラクトオリゴ糖類の給与量は、１頭当り１日
100gとした。 In addition, the amount of galacto-oligosaccharides supplied is 1 day per animal
It was 100 g.
試験結果： 試験期間中、毎日搾乳し、各個体毎に牛乳の乳脂肪率
を測定した。Test results: During the test period, milking was performed every day, and the milk fat percentage of milk was measured for each individual.
結果は下記表に示すとおりである。 The results are shown in the table below.
表にみられるとおり、ガラクトオリゴ糖類を投与した
試験区では、対照区と比べて、牛乳脂肪率の上昇が認め
られた。 As shown in the table, in the test group to which the galacto-oligosaccharide was administered, the milk fat percentage was higher than that in the control group.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 安宅 一夫 北海道札幌郡広島町高台町１丁目１番地 ４ (56)参考文献 特開 昭62−138147（ＪＰ，Ａ) ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Kazuo Ataka 1-1-1 Takadai-cho, Hiroshima-cho, Sapporo-gun, Hokkaido 4 (56) Reference JP-A-62-138147 (JP, A)
Claims (1)
ダーゼを作用させて調製したガラクトオリゴ糖類を有効
成分とする乳牛における乳脂肪率の上昇剤。1. An agent for increasing the milk fat percentage in dairy cows, which comprises a galactooligosaccharide prepared by reacting lactose or a lactose-containing substance with β-galactosidase as an active ingredient.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63051607A JP2525638B2 (en) | 1988-03-07 | 1988-03-07 | Milk fat percentage enhancer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63051607A JP2525638B2 (en) | 1988-03-07 | 1988-03-07 | Milk fat percentage enhancer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01225446A JPH01225446A (en) | 1989-09-08 |
| JP2525638B2 true JP2525638B2 (en) | 1996-08-21 |
Family
ID=12891589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63051607A Expired - Lifetime JP2525638B2 (en) | 1988-03-07 | 1988-03-07 | Milk fat percentage enhancer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2525638B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5192108B2 (en) * | 2001-09-19 | 2013-05-08 | 株式会社ヤクルト本社 | Composition for inhibiting methanogenesis and composition for feed for ruminants |
| JP5068860B2 (en) | 2008-12-22 | 2012-11-07 | 新電元工業株式会社 | Battery charger |
-
1988
- 1988-03-07 JP JP63051607A patent/JP2525638B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01225446A (en) | 1989-09-08 |
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