JP2582736B2 - Periodontal disease growth inhibitor - Google Patents

Description

The present invention relates to Ba-cteroides, which is deeply involved in the development and progression of periodontal disease and gingivitis.
gingivalis), Bacteroides intermedius (B. intermedius), Bacteroides melaninogenicus (B. melaninogenicus), Actinobacillus actinomycetemcomitans (Ac-tinobacillus actinom)
The present invention relates to a periodontal disease growth inhibitor which exhibits an excellent antibacterial action against various oral bacteria represented by ycetemcomitans), Fusobacterium nucleatum and the like.

(Prior Art) Periodontal disease is caused by progression of gingivitis, and 50% of the causes of tooth loss are periodontal disease. The prevalence of periodontal disease has increased since the late 20s, and approximately 50% of people (80% when gingivitis is involved) are affected by middle and old age. It is generally considered that gram-negative anaerobic bacilli in gingival plaque are deeply involved in the onset and progression of periodontal disease. Especially Bacteroides gingivalis (B. gingivalis), Bacteroides intermedius (B. intermedius), Bacteroides melaninogenicus (B. melaninogenicu)
s), Actinobacillus actinomycetemcomitans, Fusobacterium nucleatu
m) etc. have been suggested to be related to the onset and progression of various types of periodontal disease (Koichi Nakajima et al .: Antibiotic treatment of periodontal disease. Nihon Periodon, Vol. 29, No. 2) 463-471, 1987). Therefore, controlling bacteria involved in the development and progression of periodontal disease is an effective means in treating periodontal disease. Periodontal disease,
The use of antibiotics such as chlorhexidine, tranexamic acid, and tetracycline and minocycline is known as a drug that inhibits the growth of various oral bacteria that are greatly involved in the onset of gingivitis. , Ratania tincture, hinokitiol and the like are used.

(Problems to be Solved by the Invention) However, when these known oral bacterial growth inhibitors are used in the mouth, they have safety problems,
There were drawbacks such as an unpleasant odor when administered.

An object of the present invention is to select a substance which has antibacterial properties against periodontal disease bacteria and has no unpleasant odor from those commonly used as foods, and to provide a periodontal disease bacterial growth inhibitor comprising the same. is there.

(Means for Solving the Problems) The present invention is a growth inhibitor of periodontal disease bacteria comprising an essential oil component selected from sandalwood, guaiac, and patchouli.

The sandalwood oil used in the present invention is a known compound, for example, a slightly viscous yellow liquid obtained by steam distillation of the root and heartwood of the sandalwood family Santalum album L., which has a soft sweet woody aroma. have.

The guaiac oil used in the present invention is a known compound, for example, Bulnesia s.
Urmienti Lor. can be obtained by drying the heartwood and making it into a sawdust form by steam distillation (guaiac wood oil). At room temperature is a yellow-white solid mass, but 40-50 ° C
To a viscous yellow liquid. It has a sweet rose-like aroma and is used as a flavor.

The patchouli oil used in the present invention is a known compound, and is generally used as a flavor of water drinks by crushing dried leaves of Patchouly, a perennial plant of the Labiatae family, for example.

The dosage form of the periodontal disease growth inhibitor of the present invention can be expected to be effective when incorporated into foods such as chewing gum and candy which have a relatively long residence time in the oral cavity. The compounding amount is not necessarily uniform depending on the form and type of the food, but it is generally 0.003 to 5% by weight, preferably 0.1 to 1% by weight in the food. They may be used alone or as a mixture.

Further, the present invention can be applied not only to food but also to toothpaste, mouthwash, and the like, and is expected to be applied to treatment of periodontal disease.

Hereinafter, the present invention will be described in more detail with reference to Examples.

(Example) Prior to an example, a test method of antibacterial activity against periodontal disease bacteria will be described.

Antibacterial activity test method A modified GAM agar medium (manufactured by Nissui Pharmaceutical Co., Ltd.) containing each of the periodontal disease growth inhibitor (hereinafter referred to as “sample”) of the present invention prepared to 0.02 to 4 mg / ml by two-fold serial dilution, One platinum loop of a test bacterium that had been anaerobically cultured at 37 ° C. for 24 hours in a GAM broth medium (manufactured by Nissui Pharmaceutical Co., Ltd.) was ingested and anaerobically cultured at 37 ° C. for 5 days, and then the presence or absence of growth of the bacterium was visually determined.

The minimum concentration of the sample contained in the plate where growth was completely inhibited was defined as the minimum growth inhibition concentration (mg / ml).

In addition, Bacteroides gingivalis (Bacteroides gingivalis) ATCC33277 strain and Bacteroides intermedius (B. intermedius) ATCC15032 were used as test bacteria.
Bacteroides melaninogenikas (B. melanino
-genicus) ATCC25845 strain, Actinobacillus actinomycetem
comitans) ATCC29522 strain and Fusobacterium nucleatum ATCC10953 strain were used.

Example 1 Sandalwood oil 200 g of shredded sandalwood material and roots were subjected to steam distillation with 1 piece of water for about 50 hours to obtain about 10 g of essential oil.

Using this as a sample, an antibacterial activity test was performed by the method described above, and the results are shown in Table 1.

Example 2 Guaiac wood oil 200 g of dried heartwood of Tribulus terrestris Bulnesia surmienti Lor. Was pulverized and steam-distilled with 1 piece of water for about 50 hours to obtain about 8 g of an essential oil.

Using this as a sample, an antibacterial activity test was performed by the method described above, and the results are shown in Table 1.

Example 3 Patchouli oil 200 g of dried patchouli leaves were cut into small pieces and steam-distilled with 1 piece of water for about 8 hours to obtain about 4 g of essential oil.

Using this as a sample, an antibacterial activity test was performed by the method described above, and the results are shown in Table 1.

From the results shown in Table 1, it is clear that various flavors show an excellent effect on inhibiting the growth of bacteria.

Hereinafter, application examples of the growth inhibitor of periodontal disease bacteria of the present invention to foods will be described.

Application Example-1 (Manufacture of chewing gum) Formulation composition% by weight chewing gum base 20.0 Powdered sugar 51.9 Glucose 10.0 Ginger syrup 18.0 Sandalwood oil 0.1 Production method All the chewing gum base and all syrup candy kept at 40 ° C are put into a kneader and kneaded for 10 minutes. 1/3 of the powdered sugar and the whole amount of glucose are charged for 5 minutes, then 1/3 of the powdered sugar
Three amounts were charged and kneaded for 5 minutes. Next, sandalwood oil, which is a growth inhibitor, was mixed with the remaining 1/3 amount of powdered sugar, added, and kneaded for 5 minutes to obtain a gum mix.

Application Example 2 (Manufacture of chewing gum) Production Method A gum mix is obtained in the same manner as in Application Example 1 except that guaiac wood oil is used in an amount of 0.2% by weight in place of sandalwood oil of Application Example 1 and the powdered sugar is 51.8% by weight. Was.

Application Example-3 (Manufacture of chewing gum) Production method A gum mix was obtained in the same manner as in Application Example-1, except that 0.2% by weight of patchouli oil was used instead of sandalwood oil of Application Example-1 and powdered sugar was changed to 51.8% by weight. .

Application Example-4 (Manufacture of chewing gum) Production Method Instead of sandalwood oil of application example-1, 0.5% by weight of each of 0.1% by weight of sandalwood oil, cascia oil, cinnamon oleoresin, guaiac wood oil and patchouli oil was used. A gum mix was obtained in the same manner as in Application Example 1 except that powdered sugar was changed to 51.5% by weight.

Application Example-5 (Manufacture of candy) Formulation composition parts by weight granulated sugar 60.3 starch syrup (30% moisture) 56.0 citric acid 0.3 gardenia pigment 0.1 sandalwood oil 0.1 manufacturing method Into a pre-dissolution pot, put granulated sugar, starch syrup and a small amount of water. Bring to a boil once to completely dissolve the granulated sugar and pump this mixture into a vacuum cooker to a degree of vacuum of 46.
After boiling down at 0 mmHg and a temperature of 130 ° C., the mixture was taken into a take-out pot and transferred to a mixing pot. Then, after adding the sandalwood oil, citric acid and gardenia pigment, which are growth-inhibiting compositions, and mixing well,
Spread out on the cooling board to get candy mass.

Application Example-6 (manufacture of candy) Manufacturing method 0.2 parts by weight of guaiac wood oil instead of sandalwood oil of application example-5 and 60.2 parts of granulated sugar
A candy mass was obtained in the same manner as in Application Example-5 except that the parts by weight were used.

Application Example-7 (Manufacture of candy) Production method A candy mass was obtained in the same manner as in Application Example-5 except that 0.2 parts by weight of patchouli oil and 60.2 parts by weight of granulated sugar were used instead of sandalwood oil of Application Example-5. .

Application Example-8 (Manufacture of Candy) Manufacturing Method Application Example-5 Instead of sandalwood oil, 0.5 parts by weight of 0.1 part by weight each of sandalwood oil, cascia oil, cinnamon oleoresin, guaiac wood oil, and patchouli oil were used. A candy mass was obtained in the same manner as in Application Example-5 except that 59.9 parts by weight of granulated sugar was used.

These chewing gum candies had no unpleasant odor.

(Effect of the Invention) The growth inhibitor of periodontal disease bacteria of the present invention effectively inhibits the growth of periodontal disease bacteria that cause tooth loss. Further, it has no unpleasant odor commonly found in conventionally known growth inhibitory substances of the same bacterium, and is suitable for use in foods and other oral compositions.

Continuing from the front page Judge of the Joint Panel Koichi Kato Judge Kazuko Miyamoto Judge Hiroyuki Taniguchi (56) References JP-A-59-175410 (JP, A) JP-A-3-255031 (JP, A)

Claims (1)

(57) [Claims] 1. A growth inhibitor of periodontal disease bacteria comprising an essential oil component selected from sandalwood, guaiac and patchouli.