JP2603537B2 - Manufacturing method and apparatus for kamaboko with plate - Google Patents
Manufacturing method and apparatus for kamaboko with plateInfo
- Publication number
- JP2603537B2 JP2603537B2 JP1124519A JP12451989A JP2603537B2 JP 2603537 B2 JP2603537 B2 JP 2603537B2 JP 1124519 A JP1124519 A JP 1124519A JP 12451989 A JP12451989 A JP 12451989A JP 2603537 B2 JP2603537 B2 JP 2603537B2
- Authority
- JP
- Japan
- Prior art keywords
- kamaboko
- plate
- manufacturing
- treatment
- preventing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 42
- 238000010438 heat treatment Methods 0.000 claims description 46
- 238000000034 method Methods 0.000 claims description 35
- 238000007789 sealing Methods 0.000 claims description 28
- 244000005700 microbiome Species 0.000 claims description 27
- 230000009545 invasion Effects 0.000 claims description 26
- 230000003399 chemotactic effect Effects 0.000 claims description 21
- 210000003495 flagella Anatomy 0.000 claims description 21
- 238000004806 packaging method and process Methods 0.000 claims description 18
- 235000019465 surimi Nutrition 0.000 claims description 10
- 235000013372 meat Nutrition 0.000 claims description 7
- 241000251468 Actinopterygii Species 0.000 claims description 6
- 241001076388 Fimbria Species 0.000 claims description 6
- 239000000022 bacteriostatic agent Substances 0.000 claims description 6
- 230000003385 bacteriostatic effect Effects 0.000 claims description 4
- 230000008595 infiltration Effects 0.000 claims description 3
- 238000001764 infiltration Methods 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims 1
- 230000006866 deterioration Effects 0.000 description 44
- 241000589516 Pseudomonas Species 0.000 description 40
- 239000000047 product Substances 0.000 description 31
- 241000894006 Bacteria Species 0.000 description 28
- 238000012360 testing method Methods 0.000 description 19
- 230000002265 prevention Effects 0.000 description 10
- 238000010586 diagram Methods 0.000 description 8
- 239000003814 drug Substances 0.000 description 8
- 229940079593 drug Drugs 0.000 description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- 238000003860 storage Methods 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000012943 hotmelt Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 241000589774 Pseudomonas sp. Species 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000010025 steaming Methods 0.000 description 5
- 241000192041 Micrococcus Species 0.000 description 4
- 238000011109 contamination Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- 241000192132 Leuconostoc Species 0.000 description 3
- 101100372509 Mus musculus Vat1 gene Proteins 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 150000007524 organic acids Chemical group 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- IOYNQIMAUDJVEI-BMVIKAAMSA-N Tepraloxydim Chemical group C1C(=O)C(C(=N/OC\C=C\Cl)/CC)=C(O)CC1C1CCOCC1 IOYNQIMAUDJVEI-BMVIKAAMSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000035605 chemotaxis Effects 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 230000014670 detection of bacterium Effects 0.000 description 2
- 230000002542 deteriorative effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000004899 motility Effects 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 231100000611 venom Toxicity 0.000 description 2
- 238000003466 welding Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 206010008631 Cholera Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004831 Hot glue Substances 0.000 description 1
- 241000191936 Micrococcus sp. Species 0.000 description 1
- 241000228168 Penicillium sp. Species 0.000 description 1
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical compound CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
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- 241001148470 aerobic bacillus Species 0.000 description 1
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- DQXBYHZEEUGOBF-UHFFFAOYSA-N but-3-enoic acid;ethene Chemical compound C=C.OC(=O)CC=C DQXBYHZEEUGOBF-UHFFFAOYSA-N 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
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- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000001056 green pigment Substances 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000012858 packaging process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
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- 241000894007 species Species 0.000 description 1
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- 239000000057 synthetic resin Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Landscapes
- Fish Paste Products (AREA)
Description
【発明の詳細な説明】 [産業上の利用分野] 本発明は折込み部分が板下面上になるよう全体を包装
された板付蒲鉾の軟化による変敗を防止するための蒲鉾
の製造方法及びその装置に関するものである。更に詳し
く付言するならば、軟化の原因となる微生物を新たに提
言し、該微生物の性状に基いた防止するための製造方法
及びその装置を提示するものである。本発明で開示する
具体的な微生物は、鞭毛又は線毛を有する走化性微生物
の一つであるシュードモナス菌(Pseudomonas sp.)で
ある。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method and an apparatus for manufacturing a kamaboko for preventing deterioration due to softening of a kamaboko with a board, the whole of which is folded so that the folded portion is on the lower surface of the board. It is about. More specifically, the present invention proposes a new microorganism that causes softening, and presents a production method and an apparatus for preventing the microorganism based on the properties of the microorganism. A specific microorganism disclosed in the present invention is Pseudomonas sp., Which is one of chemotactic microorganisms having flagella or pili.
[従来の技術] 食塩、澱粉、化学調味料を加えて練り潰した魚肉のす
り身を板の上にのせ、蒸気等で加熱してつくられた板付
蒲鉾には、加熱の工程により蒸し蒲鉾(リテーナ蒲鉾を
含む)、焼板蒲鉾、焼抜き蒲鉾がある。[Prior art] On a plate, a fish meat surimi made by adding salt, starch, and a chemical seasoning is ground, and then heated with steam or the like. (Including kamaboko), baking plate kamaboko and grilled kamaboko.
蒸し蒲鉾は、板の上にすり身をのせ、蒸してから包装
する(あるいは包装してから蒸す)又はリテーナ包装後
蒸してつくる。焼板蒲鉾は蒸した後、表面だけを焼く、
焼抜き蒲鉾は、板面から焼いた後に肉面を焼いてつく
る。Steamed kamaboko is prepared by placing a surimi on a plate, steaming and packaging (or steaming after packaging), or steaming after packaging the retainer. After steaming the roasted kamaboko, bake only the surface,
Grilled kamaboko is made by baking the meat after baking from the plate.
現在では板付蒲鉾は、蒸し蒲鉾が主流となり、製品の
細菌汚染を考慮して従来の製法である板の上にすり身を
つけ、坐り処理後、蒸した後、折込み包装して冷却する
製法(以下、小田原蒲鉾と称す)ではなく、板の上にす
り身をつけ、リテーナ包装後、加熱冷却し外装をかける
工法(以下、リテーナ蒲鉾と称す)と、板の上にすり身
をつけ、坐り処理後、折込み包装して蒸した後、冷却す
る工法(以下、折込み蒲鉾と称す)とが主に行われてい
る。第7a図,第7b図は前記2つの工法による完成品の下
斜め方向からの斜視図である。第7a図はリテーナ蒲鉾、
第7b図は折込み蒲鉾を示す。図に示す通り2つの方法に
よる完成品は同様に板下面状に折込み部分がある。At present, steamed kamaboko has become the mainstream, and in consideration of bacterial contamination of the product, the surimi is put on a plate, which is a conventional manufacturing method, and after sitting, steamed, folded, wrapped and cooled (hereinafter referred to as a cooling method) , Not Odawara Kamaboko), instead of putting a surimi on the board, after packaging the retainer, heating and cooling and applying an exterior (hereinafter referred to as a retainer Kamaboko), put the surimi on the board, and after sitting, The method of folding and packaging, steaming, and then cooling (hereinafter referred to as folding kamaboko) is mainly performed. FIGS. 7a and 7b are perspective views of the completed product obtained by the above-mentioned two construction methods as viewed from the lower oblique direction. Figure 7a shows a retainer kamaboko,
FIG. 7b shows a folding kamaboko. As shown in the figure, the finished product by the two methods similarly has a folded portion on the lower surface of the plate.
[発明が解決しようとする課題] しかしながら、折込み部分が板下面上になるよう包装
した板付蒲鉾では、リテーナ蒲鉾や折込み蒲鉾のように
加熱前に包装した場合には、ネトやカビ等の原因となる
細菌の繁殖は少ないが、10℃以下の低温保管中におい
て、軟化による変敗があり、より長期の保存に耐え得る
製品の作成が望まれていたが、その原因は確認されてい
ないのが現状であった。[Problems to be Solved by the Invention] However, in the case of a kamaboko with a plate packaged so that the folded portion is on the lower surface of the plate, if it is wrapped before heating like a retainer kamaboko or a folded kamaboko, it may cause problems such as neto and mold. Although the growth of bacteria is small, there is deterioration due to softening during storage at a low temperature of 10 ° C or less, and it has been desired to create a product that can withstand long-term storage, but the cause has not been confirmed. It was the current situation.
本発明においては、低温保管中における板付蒲鉾を変
敗させる原因となる菌をつきとめ、該菌の特性を明確に
することにより、該菌の侵入・繁殖の防止により板付蒲
鉾の変敗を防止するための製造方法及びその装置を得る
ことを目的とする。In the present invention, the bacteria that cause deterioration of the plate-shaped kamaboko during cold storage are identified and the characteristics of the bacteria are clarified, thereby preventing the invasion and propagation of the bacteria to prevent the deterioration of the plate-shaped kamaboko. To obtain a manufacturing method and an apparatus therefor.
[課題を解決するための手段] 第1発明に係る板付蒲鉾の製造方法では、魚肉等のす
り身を板の上面に載せて調理し、折り込み部分が前記板
の下面上になるように全体を実質的に無菌状態で包装し
てなる板付蒲鉾の製造方法において、 包装後に、前記折り込み部分のある板下面部のみに、
鞭毛又は線毛を有する走化性微生物の前記折り込み部分
への侵入を阻止する処理を施すものである。本発明に開
示する具体的な鞭毛又は線毛を有する走化性微生物は、
シュードモナス菌(Pseudomonas sp.)である。[Means for Solving the Problems] In the method for manufacturing a kamaboko with a plate according to the first invention, the surimi such as fish meat is placed on the upper surface of the plate and cooked, and the whole is substantially formed so that the folded portion is on the lower surface of the plate. In a method of manufacturing a kamaboko with a plate, which is packaged in a sterile condition, after packaging, only on the lower surface of the plate with the folded portion,
A process for preventing chemotactic microorganisms having flagella or fimbriae from entering the folded portion. Specific flagella or pili-containing chemotactic microorganisms disclosed in the present invention,
Pseudomonas sp.
第2発明に係る板付蒲鉾の製造方法では、前記第1発
明に記載の板付蒲鉾の製造方法において、 前記侵入を阻止する処理が、前記折り込み部分のある
板下面を60℃以上に加熱する加熱処理であることを特徴
とするものである。In the method for manufacturing a kamaboko with a plate according to the second invention, in the method for manufacturing the kamaboko with a plate according to the first invention, the treatment for preventing intrusion is a heat treatment for heating the lower surface of the plate with the folded portion to 60 ° C. or more. It is characterized by being.
第3発明に係る板付蒲鉾の製造方法では、前記第1発
明に記載の板付蒲鉾の製造方法において、 前記侵入を阻止する処理が、前記板下面の折り込んで
重ね合わせられた部分を小口方向と長手方向とをシール
する前記走化性微生物の侵入経路を塞ぐ処理であること
を特徴とするものであり、本実施例で開示する具体的な
処理方法は、前記走化性微生物の侵入経路を塞ぐ処理
が、ホットメルトシール方又は超音波溶着方である。In the method for manufacturing a kamaboko with a plate according to a third invention, in the method for manufacturing a kamaboko with a plate according to the first invention, the step of preventing the intrusion comprises: This is a process for closing the invasion route of the chemotactic microorganism, which seals the direction, and a specific treatment method disclosed in this embodiment is to block the invasion route of the chemotactic microorganism. The treatment is a hot melt sealing method or an ultrasonic welding method.
第4発明に係る板付蒲鉾の製造方法では、前記第1発
明に記載の板付蒲鉾の製造方法において、 前記侵入を阻止する処理が、前記折り込み部分のある
板下面の外装上からの静菌剤の浸潤による前記走化性微
生物の静菌処理であることを特徴とするものであり、本
実施例で開示する具体的な処理方法は、前記静菌剤が有
機酸系製剤、アルコール系製剤又は酸素系薬剤である。In the method for manufacturing a kamaboko with a plate according to the fourth invention, in the method for manufacturing a kamaboko with a plate according to the first invention, the treatment for preventing intrusion is performed by removing the bacteriostatic agent from the exterior of the lower surface of the plate with the folded portion. It is characterized in that it is a bacteriostatic treatment of the chemotactic microorganism by infiltration, a specific treatment method disclosed in the present embodiment, the bacteriostatic agent is an organic acid-based formulation, alcohol-based formulation or oxygen It is a system drug.
第5発明に係る板付蒲鉾の製造装置では、魚肉等のす
り身を板の上面に載せて調理し、折り込み部分が前記板
の下面上になるように全体を実質的に無菌状態で包装し
てなる板付蒲鉾の製造装置において、 包装後に、前記折り込み部分のある板下面部のみに、
鞭毛または線毛を有する走化性微生物の前記折り込み部
分への侵入を阻止する処理を施す装置を備えたものであ
る。本発明に開示する具体的な鞭毛又は線毛を有する走
化性微生物は、シュードモナス菌(Pseudomonas sp.)
である。In the apparatus for manufacturing a kamaboko with a plate according to the fifth invention, the surimi such as fish meat is placed on the upper surface of the plate and cooked, and the whole is substantially aseptically packaged so that the folded portion is on the lower surface of the plate. In the apparatus for manufacturing a kamaboko with a plate, after packaging, only the lower surface portion of the plate with the folded portion is provided.
An apparatus for performing a treatment for preventing entry of chemotactic microorganisms having flagella or fimbria into the folded portion. Specific chemotactic microorganisms having flagella or pili disclosed in the present invention are Pseudomonas sp.
It is.
第6発明に係る板付蒲鉾の製造装置では、前記第5発
明に記載の板付蒲鉾の製造装置において、 前記侵入を阻止する処理を施す装置が、前記折り込み
部分のある板下面を60℃以上に加熱する加熱装置である
ことを特徴とするものである。In the apparatus for manufacturing a kamaboko with a plate according to the sixth invention, in the apparatus for manufacturing a kamaboko with a plate according to the fifth invention, the apparatus for performing the treatment for preventing intrusion heats a lower surface of the plate with the folded portion to 60 ° C. or more. It is characterized by being a heating device to perform.
第7発明に係る板付蒲鉾の製造装置では、前記第5発
明に記載の板付蒲鉾の製造装置において、 前記侵入を阻止する処理を施す装置が、前記板下面の
折り込んで重ね合わせられた部分を小口方向と長手方向
とをシールする密閉装置であることを特徴とするもので
ある。In the apparatus for manufacturing a kamaboko with a plate according to the seventh invention, in the apparatus for manufacturing a kamaboko with a plate according to the fifth invention, the apparatus for performing the treatment for preventing the invasion includes a fore edge of the folded and overlapped portion of the lower surface of the plate. It is a sealing device for sealing a direction and a longitudinal direction.
第8発明に係る板付蒲鉾の製造装置では、前記第5発
明に記載の板付蒲鉾の製造装置において、 前記侵入を阻止する処理を施す装置が、静菌剤を前記
折り込み部分のある板下面の外装上から浸潤させる装置
であることを特徴とするものである。In the apparatus for manufacturing a kamaboko with a plate according to an eighth invention, in the apparatus for manufacturing a kamaboko with a plate according to the fifth invention, the apparatus for performing the treatment for preventing the invasion comprises: It is a device for infiltrating from above.
[作用] 本発明においては、低温保管中における板付蒲鉾を変
敗(軟化型変敗)させる原因となる菌は、シュードモナ
ス菌(Pseudomonas sp.)であることを突き止めた。[Action] In the present invention, it has been found that the bacterium that causes deterioration (softening type deterioration) of the boarded kamaboko during cold storage is Pseudomonas sp.
シュードモナス菌は、グラム陰性有ベン毛桿菌であ
り、鞭毛を有し、運動性(走化性)を有する。このた
め、加熱工程以降のライン上から板下面の折込み部分か
ら侵入して、製品部分に泳ぎ付き、製品部品で繁殖し
て、板付蒲鉾を軟化変敗させることが判明した。Pseudomonas is a Gram-negative venomous bacillus, has flagella, and has motility (chemotaxis). For this reason, it turned out that it invades from the folding part of the board lower surface from the line after a heating process, swims in a product part, breeds with a product part, and softens and degrades the board-mounted kamaboko.
本第1及び第5発明ではこの鞭毛又は線毛を有する走
化性微生物の特性を考慮して、該菌の侵入・繁殖を防止
することにより蒲鉾の軟化変敗を防止する製造方法及び
装置を開示する。In the first and fifth inventions, there is provided a method and apparatus for preventing softening and deterioration of a kamaboko by preventing the invasion and propagation of chemotactic microorganisms having flagella or fimbriae in consideration of the characteristics of the chemotactic microorganisms. Disclose.
本第2〜第4及び第6〜第8発明では具体的な鞭毛又
は線毛を有する走化性微生物の侵入・繁殖を防止する製
造方法及び装置には、次の3通りを開示するものであ
る。In the present second to fourth and sixth to eighth inventions, the following three methods are disclosed as specific production methods and apparatuses for preventing invasion and propagation of chemotactic microorganisms having flagella or pili. is there.
(1)加熱殺菌;変敗を起す鞭毛又は線毛を有する走化
性微生物の一つであるシュードモナス菌は、具体的には
60℃でその殆どが死滅する。このため、包装後に板の下
面から折込み部分に対して加熱処理を施すことにより前
記シュードモナス菌の侵入を防止する。しかも加熱部分
は、折込み部分のある板下面上のみであるため、加熱に
よる蒲鉾の品質の低下はない。(1) Heat sterilization; Pseudomonas bacteria, one of the chemotactic microorganisms having flagella or fimbriae that cause deterioration,
Most of them die at 60 ° C. For this reason, by applying a heat treatment to the folded portion from the lower surface of the plate after packaging, the invasion of the Pseudomonas bacteria is prevented. Moreover, since the heating portion is only on the lower surface of the plate having the folded portion, the quality of the kamaboko does not deteriorate due to heating.
(2)密封包装;変敗を起す鞭毛又は線毛を有する走化
性微生物の一つであるシュードモナス菌は、前記の通り
60℃以上では死滅するため従来は製品が低温となるライ
ン上で汚染される。このため、製品が前記シュードモナ
ス菌に汚染される前に包装後に板の下面から折込み部分
に対して、折込み部分を密閉する処理を施すことにより
ライン上からの汚染を防止する。具体的には折込み部分
をホットメルトシール法又は超音波溶着法で密閉するこ
とを開示しているが、これに限定するものではない。(2) Hermetically sealed packaging; Pseudomonas bacterium, which is one of chemotactic microorganisms having flagella or fimbria that causes deterioration, is as described above.
At 60 ° C or higher, the product will die and the product will be contaminated on the line where the temperature is low. Therefore, before the product is contaminated by the Pseudomonas bacterium, by performing a process of sealing the folded portion from the lower surface of the plate after packaging and preventing the contamination from the line. Specifically, it discloses that the folded portion is sealed by a hot melt sealing method or an ultrasonic welding method, but the present invention is not limited to this.
(3)薬剤による静菌殺菌;変敗を起す鞭毛又は線毛を
有する走化性微生物の一つであるシュードモナス菌が侵
入する折込み部分を3薬剤により浸潤させることによ
り、前記シュードモナス菌の繁殖を防止して汚染を防止
する。浸潤させる薬剤は食品であることを考慮して具体
的には有機酸系製剤、アルコール系製剤又は酵素系薬剤
を開示しているが、これに限定するものではない。(3) Bacteriostatic sterilization by a drug; the invasion of the pseudomonas bacterium, which is one of the chemotactic microorganisms having flagella or fimbriae causing deterioration, by infiltrating the folded portion with the three drugs, thereby promoting the propagation of the Pseudomonas bacterium. Prevent and prevent pollution. In view of the fact that the drug to be infiltrated is food, an organic acid-based preparation, an alcohol-based preparation or an enzyme-based preparation is specifically disclosed, but the present invention is not limited thereto.
また、本実施例では蒲鉾内に侵入した変敗を起す鞭毛
又は線毛を有する走化性微生物としてシュードモナス菌
を検出したが、本発明が対象とする蒲鉾内に侵入する変
敗を起す鞭毛又は線毛を有する走化性微生物は、シュー
ドモナス菌に限らず、(製造ライン上にあってはならな
い菌であるが)他に鞭毛を有する菌として、チフス菌、
赤痢菌、コレラ菌、線毛を有する菌として大腸菌等があ
り、走化性を有している細菌及び微生物で製品である蒲
鉾に影響を与え得るもの全てである。Further, in the present embodiment, Pseudomonas bacteria were detected as chemotactic microorganisms having decaying flagella or fimbria invading the kamaboko, but the flagella or the decaying flagella invading the kamaboko targeted by the present invention were detected. Chemotaxis microorganisms having pili are not limited to Pseudomonas bacteria, but are bacteria that have other flagella (although they must not be on the production line), such as Salmonella typhi,
There are Escherichia coli and the like as Shigella, cholera, and fimbriae having pilus, and they are all chemotactic bacteria and microorganisms that can affect the product kamaboko.
[実施例] 以下に本発明の実施例を示す。[Example] An example of the present invention will be described below.
実験例1(蒲鉾変敗菌の同定) 低温保管中における蒲鉾の変敗について検討した。市
販蒲鉾を低温(10℃)で保管し変敗させた後、変敗原因
菌を分離し、駒形の好気性細菌の同定法(駒形和男,
「化学と生物」Vol.10,No.5))によった。Experimental Example 1 (Identification of spoilage of Kamaboko) The deterioration of Kamaboko during cold storage was examined. After storing commercially available kamaboko at a low temperature (10 ° C) and decomposing it, the bacteria causing the spoilage are separated, and a method for identifying Komagata aerobic bacteria (Kazuo Komagata,
"Chemistry and biology" Vol.10, No.5)).
結果を次の第1表に示す。 The results are shown in Table 1 below.
第1表に示すように、市販蒲鉾を購入し低温保管した
後の変敗発生形態は(1)ネト型、(2)軟化型、
(3)カビ型であった。(1)、(3)のネト及びカビ
型は過去に報告のあるように透明や黄色等のネトや暗緑
色のカビであり、変敗原因菌は乳酸菌(Leuconostoc s
p.)、ミクロコッカス菌(Micrococcus sp.)、ペニシ
リウム菌(Penicillium sp.)等であった。(2)の軟
化型は包装紙の折りこみ部に黄緑色の蛍光を呈し蒲鉾表
面を軟化させるタイプと、単に蒲鉾表面を軟化させるタ
イプとであった。これらは過去に報告例がないものであ
り、軟化型変敗原因菌を同定した結果、全てシュードモ
ナス菌の単一菌叢であった。 As shown in Table 1, after the commercial kamaboko was purchased and stored at low temperature, the deterioration occurred in (1) net-type, (2) softening-type,
(3) Mold type. The neto and mold types (1) and (3) are netos such as transparent or yellow and dark green mold as reported in the past, and the decay-causing bacteria are lactic acid bacteria (Leuconostoc s).
p.), Micrococcus sp., Penicillium sp. The softening type of (2) was of a type in which yellowish green fluorescence was exhibited at the folded portion of the wrapping paper to soften the surface of the kamaboko, or a type in which the surface of the kamaboko was simply softened. These have not been reported in the past, and as a result of identifying the causative bacteria of the softening type, all of them were single flora of Pseudomonas.
実験例2(蒲鉾変敗菌の軟化再現試験) 軟化変敗蒲鉾から分離したシュードモナス菌及び蒲鉾
製造工程の加熱処理以降から検出したシュードモナス菌
を用いて軟化再現試験をした。Experimental Example 2 (Reproduction test of softening and decomposing bacteria) Softening reproduction test was performed using Pseudomonas bacteria isolated from softening and degrading Kamaboko and Pseudomonas bacteria detected after the heat treatment in the Kamaboko manufacturing process.
第1a図・第1b図は軟化再現試験操作を示す説明図であ
る。具体的には、a図に示すように、バット1内にガー
ゼ2を敷き、バット1内にガーゼ2を浸す程度の菌液3
(約20ml)を入れた。菌液中の菌数のオーダは102〜102
cells/ml程度とした。バット1内に板付蒲鉾4を2分間
静置して菌液を付着させた後、b図に示すように、ネッ
ト5上に1時間放置して被験蒲鉾とした。1a and 1b are explanatory views showing a softening reproduction test operation. Specifically, as shown in Fig. A, the gauze 2 is laid in the vat 1 and the bacterial solution 3 is soaked that the gauze 2 is dipped in the vat 1.
(About 20 ml). The order of the number of bacteria in the bacterial solution is 10 2 to 10 2
It was about cells / ml. After the kamaboko 4 with the plate was allowed to stand in the vat 1 for 2 minutes to allow the bacterial solution to adhere, it was left on the net 5 for 1 hour as shown in FIG.
保管温度10℃で30日間保管後、変敗の有無を確認後、
シュードモナス菌検出培地としてNAC(NAC寒天培地、栄
研化学(株)製)寒天培地、ミクロコッカス菌検出培地
としてSPC(標準寒天培地、栄研化学(株)製)寒天培
地(コロニーの色にて判断)、乳酸菌(Leuconostoc s
p.)検出培地としてBCP(BCP加プレートカウント寒天培
地、栄研化学(株)製)平板培地を各々使用して、フキ
トリ検査を行い、変敗菌の侵入を確認した。After storage at a storage temperature of 10 ° C for 30 days, check for deterioration,
NAC (NAC agar medium, manufactured by Eiken Chemical Co., Ltd.) agar medium as a Pseudomonas detection medium, SPC (standard agar medium, manufactured by Eiken Chemical Co., Ltd.) agar medium (by colony color) as a micrococcus detection medium Judgment), lactic acid bacteria (Leuconostoc s)
p.) BCP (plate counting agar medium with BCP, manufactured by Eiken Chemical Co., Ltd.) plate medium was used as a detection medium, and a footprint test was carried out to confirm the invasion of degrading bacteria.
前記フキトリ検査は以下の操作による。 The footwear inspection is performed by the following operation.
(1)蒲鉾の折込み包装の外側を殺菌剤(70%エタノー
ル)で拭いて殺菌した。(1) The outside of the folded package of the Kamaboko was sterilized by wiping with a bactericide (70% ethanol).
(2)包装紙を剥ぎ取り、無菌綿棒で製品表面を拭き取
り塗布した菌種に合せてNAC、SPC、BCP培地にストリー
クした。(2) The wrapping paper was peeled off, and the surface of the product was wiped off with a sterile cotton swab and streaked on NAC, SPC, and BCP media according to the applied bacterial species.
(3)25〜30℃で2〜4日培養後前記各培地に生育した
菌を確認。(3) After culturing at 25 to 30 ° C. for 2 to 4 days, bacteria grown on each of the above-mentioned media were confirmed.
(4)更に必要に応じて、各培地に生育したコロニーを
駒形の同定法により同定して判定した。(4) Further, if necessary, colonies grown on each medium were identified and determined by a peg-shaped identification method.
結果を次の第2表に示す。尚、表中の侵入率は(侵入
率/サンプル数)変敗率は(変敗数/サンプル数)を示
す。The results are shown in Table 2 below. The penetration rate in the table indicates (penetration rate / number of samples) and the deterioration rate indicates (deterioration number / number of samples).
第2表から変敗原因菌は全て包装紙の折り込み部分よ
り侵入することが確認出来た。また、シュードモナス菌
は、グラム陰性有ベン毛桿菌であり、端在性の鞭毛を有
し、運動性を有する等の菌の性質からも判断できるよう
に、侵入率、変敗率も多かった。 From Table 2, it was confirmed that all the bacteria causing deterioration were invaded from the folded portion of the wrapping paper. Pseudomonas bacteria are Gram-negative venomous bacilli, have terminal flagella, have motility, and have a high invasion rate and a high rate of spoilage as can be judged from the properties of the bacteria.
包装形態では、リテーナ蒲鉾より折込み蒲鉾の方がや
や侵入しやすかった。In the packaging form, the folded kamaboko was slightly easier to invade than the retainer kamaboko.
ミクロコッカス菌より乳酸菌(Leuconostoc)の方が
折込み蒲鉾でやや侵入率が高かった。シュードモナス菌
及び乳酸菌では変敗が見られたが、ミクロコッカス菌で
は全く変敗が認められなかった。これは、ミクロコッカ
ス菌が蒲鉾上で増殖が遅いためと推定された。Lactic acid bacteria (Leuconostoc) had a slightly higher invasion rate with folded kamaboko than micrococcus bacteria. Deterioration was observed in Pseudomonas bacteria and lactic acid bacteria, but no degradation was observed in Micrococcus bacteria. This was presumed to be due to slow growth of the micrococcus bacteria on the kamaboko.
実験例3(加熱処理による変敗防止1) 実験例2の方法で製品外装(蒲鉾板部分)にシュード
モナス菌を付着させた後、ホットプレートにて製品底部
(蒲鉾板部分)を加熱した後、前記フキトリ検査でシュ
ードモナス菌の残存について調べた。Experimental Example 3 (Prevention of deterioration by heat treatment 1) After attaching Pseudomonas bacteria to the product exterior (Kamaboko plate portion) by the method of Experimental example 2, heating the product bottom (Kamaboko plate portion) with a hot plate, In the above-mentioned husky test, the residual Pseudomonas was examined.
第1図及び実験例2に示した被験蒲鉾を15サンプル用
意し、その内の5サンプルを130℃のホットプレートに
1分間静置する加熱工程を行い、無処理の10サンプルと
比較して加熱処理変敗テストを行った。尚、加熱終了後
の製品底部の温度は65〜70℃であった。A heating step was performed in which 15 samples of the test kamaboko shown in FIG. 1 and Experimental Example 2 were prepared, and 5 of the samples were allowed to stand on a hot plate at 130 ° C. for 1 minute. A processing deterioration test was performed. The temperature at the bottom of the product after the completion of the heating was 65 to 70 ° C.
結果は次の第3表に示す。尚、表中aは製品外装全面
(蒲鉾板部分の外装全面)に、bは折込部(蒲鉾板部分
の外装内部)に残存しているかを検査したものである。The results are shown in Table 3 below. In the table, a is a test for checking whether the product remains on the entire surface of the product exterior (the entire surface of the Kamaboko plate portion), and b is a test for checking whether the product remains in the folded portion (inside the exterior of the Kamaboko plate portion).
第3表に示すように、製品外装及び折込み部まで達し
ているシュードモナス菌も65〜70℃に製品底部がなって
いれば殺菌されて変敗を防止することができることが解
った。 As shown in Table 3, it was found that Pseudomonas bacteria reaching the product exterior and the folded part were sterilized if the product bottom was at 65 to 70 ° C., so that deterioration could be prevented.
実施例4(加熱処理による変敗防止2) 遠赤外線加熱装置を使用して、同様に加熱処理変敗テ
ストを行った。尚、加熱条件を次の4種類に分けて実施
した。Example 4 (Prevention of deterioration by heat treatment 2) A heat treatment deterioration test was similarly performed using a far-infrared heating device. The heating conditions were divided into the following four types.
A;遠赤外線加熱装置温度400℃,照射時間120秒,距離10
cm B;遠赤外線加熱装置温度400℃,照射時間60秒,距離10c
m C;遠赤外線加熱装置温度400℃,照射時間60秒,距離5cm D;遠赤外線加熱装置温度400℃,照射時間30秒,距離5cm 各々の中心温度(蒲鉾内部温度)と非接触温度(板下
面部分の温度)の数値は次の第4表に示す。A; Far-infrared heating device temperature 400 ° C, irradiation time 120 seconds, distance 10
cm B; Far infrared heating device temperature 400 ° C, irradiation time 60 seconds, distance 10c
m C; Far-infrared heating device temperature 400 ° C, irradiation time 60 seconds, distance 5cm D; Far-infrared heating device temperature 400 ° C, irradiation time 30 seconds, distance 5cm Each center temperature (Kamaboko internal temperature) and non-contact temperature (plate The values of (temperature of the lower surface portion) are shown in Table 4 below.
第4表に示す条件で各5サンプルについてフキトリ検
査によりシュードモナス菌の残存を調べた。 Under the conditions shown in Table 4, the residual of Pseudomonas bacteria was examined for each of the five samples by a footprint test.
具体的な操作は、前述と同様に実験例2方法で蒲鉾下
面部外装にシュードモナス菌を付着させた後、前記4つ
の条件ごとに遠赤外線加熱装置にて製品底部(蒲鉾板部
分)を加熱した後、前記フキトリ検査でシュードモナス
菌の残存について調べた。The specific operation was as follows. After attaching Pseudomonas bacteria to the outer surface of the bottom of the kamaboko by the method of Experimental Example 2 in the same manner as described above, the bottom of the product (the kamaboko board) was heated with a far-infrared heating device for each of the above four conditions. Then, the residual Pseudomonas bacteria were examined by the above-mentioned husk test.
結果を第5表に示す。尚、表中aは製品外装全面(蒲
鉾板部分の外装全面)、bは折込み部(蒲鉾板部分の外
装内部)に残存する菌数を示す。The results are shown in Table 5. In the table, a indicates the number of bacteria remaining on the entire product exterior (the entire exterior of the Kamaboko plate portion), and b indicates the number of bacteria remaining in the folded portion (inside the exterior of the Kamaboko plate portion).
第5表に示す通り、条件A〜Cではシュードモナス菌
は全く確認されず、条件Dでのみ確認された。条件Dの
非接触温度(板下面部分の温度)が62〜58℃では、完全
に殺菌することができなかったことから、前記A〜C処
理の条件からシュードモナス菌は60℃以上に加熱すれば
確実に殺菌されることを確認した。 As shown in Table 5, no Pseudomonas bacteria were confirmed under the conditions A to C, and only under the condition D. When the non-contact temperature (temperature of the lower surface portion of the plate) under the condition D was 62 to 58 ° C., it was not possible to completely sterilize the pseudomonas bacteria. It was confirmed that sterilization was sure.
以上の結果より、蒲鉾生産装置から梱包装置に至るラ
イン上へ、前記の様な遠赤外線加熱装置及びホットプレ
ート等の加熱変敗防止処理装置を敷設することにより、
蒲鉾製品の変敗防止が行えることが確認された。From the above results, on the line from the Kamaboko production device to the packing device, by laying the above-mentioned far-infrared heating device and a heating / deterioration prevention treatment device such as a hot plate,
It was confirmed that the Kamaboko product could be prevented from deteriorating.
第2図は蒲鉾製造装置から外装・梱包装置に至るライ
ン上に敷設された遠赤外線加熱装置による変敗防止装置
の一実施例を示す概略図である。図において、魚肉等の
すり身を板上面に載せて調理し、折込み部分が板下面上
になるよう全体をリテーナ包装された板付蒲鉾10が折込
み部分を底部として連続して搬出されて来る上流ベルト
コンベア11の終了端に、敷設されたネット12の下方に、
遠赤外線加熱装置13が照射口を上方に向けて設置され
る。加熱温度は、遠赤外線加熱装置温度,照射時間,照
射距離等を調節して行い、具体的には折込み部分が60℃
以上であればよい。遠赤外線加熱装置13によって加熱さ
れた板付蒲鉾10は下流コンベア14により搬送され、外包
装・梱包を施されて出荷される。FIG. 2 is a schematic view showing an embodiment of a deterioration preventing device using a far-infrared heating device laid on a line from a kamaboko manufacturing device to an exterior / packaging device. In the figure, an upstream belt conveyor in which a surimi of fish meat or the like is placed on the upper surface of the plate and cooked, and the kamaboko 10 with a plate, which is entirely packaged with a retainer so that the folded portion is on the lower surface of the plate, is continuously taken out with the folded portion as the bottom. At the end of 11, below the laid net 12,
The far-infrared heating device 13 is installed with the irradiation port facing upward. The heating temperature is controlled by adjusting the temperature of the far-infrared heating device, irradiation time, irradiation distance, etc.
All that is required is the above. The kamaboko with a plate 10 heated by the far-infrared heating device 13 is conveyed by the downstream conveyor 14, and is externally wrapped and packed before being shipped.
当然のことであるが下流コンベア以降は少なくとも製
品底部(板下面部)は無菌状態で搬送・包装・梱包され
なければならない。尚、本発明は第2図に示した装置に
限定されることなく、加熱装置も外包装が終了した時期
に行ってもよいし、加熱方法もホットプレートで行って
もよい。また、後述の他の変敗防止装置と適宜組合わせ
てもよい。As a matter of course, after the downstream conveyor, at least the bottom of the product (the lower surface of the plate) must be transported, packaged, and packed in an aseptic state. The present invention is not limited to the apparatus shown in FIG. 2, and the heating device may be performed at the time when the outer packaging is completed, or the heating method may be performed using a hot plate. Further, it may be appropriately combined with another deterioration prevention device described later.
実験例5(密閉処理による変敗防止1) 蒲鉾へのシュードモナス菌侵入防止法の1つとして、
通常包装後、更にホットメルト法により密封シールする
方法を検討した。Experimental Example 5 (Prevention of deterioration by sealing treatment 1) As one of the methods for preventing Pseudomonas invasion into Kamaboko,
After normal packaging, a method of hermetically sealing by a hot melt method was further studied.
冷却後の最終製品(折込み蒲鉾)をサンプルとしてホ
ットメルト接着ガン(ヘンケル白水(株)製)を用いて
手作業にてシールした。第3a図,第3b図はシールの状態
を示す説明図であり、図に示すようにセンター部分20と
その両端の小口部分21をシールしたものを被験体Aと
し、2つの小口部分21′のみをシールしたものを被験体
Bとした。The cooled final product (folded kamaboko) was used as a sample, and sealed by hand using a hot melt adhesive gun (manufactured by Henkel Hakusui Co., Ltd.). FIGS. 3a and 3b are explanatory views showing the state of the seal. As shown in the figure, the center portion 20 and the fore-edge portions 21 at both ends are sealed, and the subject A is designated as a subject A, and only two fore-edge portions 21 'are shown. Was sealed as a subject B.
使用ホットメルトスティックは酢酸ビニル樹脂系接着
剤(合成樹脂40%、エチレン酢酸ビニル樹脂共重合物石
油系樹脂30%、ワックス30%)であった。The hot melt stick used was a vinyl acetate resin-based adhesive (synthetic resin 40%, ethylene vinyl acetate resin copolymer petroleum resin 30%, wax 30%).
シールの確認は色素液テストにより確認した。具体的
な色素液テストの操作は、バット内にガーゼを敷き、バ
ット内にガーゼを浸す程度の色素液(1%メチレンブル
ーを入れ、ガーゼ上に蒲鉾を置き、上から軽く抑えて色
素を塗布した。常温で3時間放置後0℃で更に1日静置
させて放置し色素液の侵入を肉眼で確認した。The seal was confirmed by a dye solution test. The specific operation of the dye solution test was to spread the gauze in the vat, apply the dye solution to the extent that the gauze was immersed in the vat (add 1% methylene blue, place the kamaboko on the gauze, and apply the dye while lightly suppressing it from above. After leaving at room temperature for 3 hours, the mixture was allowed to stand still at 0 ° C. for 1 day, and then left standing, and the invasion of the dye solution was visually confirmed.
結果は次の第6表に示す。尚、表中−は色素が侵入し
なかったこと、+は折込み部に侵入したこと、++は折
込み部の小口部分にまで侵入したことを示す。The results are shown in Table 6 below. In the table,-indicates that the dye did not enter, + indicates that it entered the fold, and ++ indicates that it entered the small edge of the fold.
第6表に示す通り、Aタイプのホットメルトシールを
すれば、色素液は侵入せず、完全に密封することがで
き、シュードモナス菌の侵入を防止することができるこ
とが判明した。また、リテーナ蒲鉾においても、同様
に、センター部分と小口部分を密封することによりシュ
ードモナス菌の侵入を防止することができるものと思わ
れた。 As shown in Table 6, it was found that if the type A hot melt seal was used, the dye solution did not enter and the solution could be completely sealed, thereby preventing the invasion of Pseudomonas bacteria. Similarly, it was considered that the invasion of Pseudomonas bacteria could also be prevented by sealing the center portion and the fore-end portion in the retainer kamaboko.
実験例6(密閉処理による変敗防止2) 実験例5の密閉処理による変敗防止が良好であったた
め、超音波シール装置により蒲鉾の密封シールする方法
を検討した。Experimental Example 6 (Prevention of deterioration by sealing treatment 2) Since the deterioration prevention by the sealing treatment of Experiment 5 was good, a method of hermetically sealing Kamaboko with an ultrasonic sealing device was studied.
第4a図,第4b図はシールの状態を示す説明図であり、
図に示すようにセンター部分30とその両端に垂直の小口
部分31をシールしたものを被験体Aとし、2つの小口部
分31′のみをシールしたものを被験体Bとした。4a and 4b are explanatory views showing the state of the seal,
As shown in the figure, the subject A was sealed with the center portion 30 and the fore-edge portions 31 perpendicular to both ends thereof, and the subject B was obtained by sealing only the two fore-edge portions 31 '.
第5図は第4図センター部分をシールする超音波シー
ル装置の説明図、第6図は第4図小口部分をシールする
超音波シール装置の説明図である。第5図に示すよう
に、長手方向に移動しているフィルム包装された板付蒲
鉾40の板41下面中央部の重ね合せ部分42を回転させた円
盤ホーン43に接触させる。円盤ホーン43は発振機44に接
続された振動子45からの超音波をフィルムに伝達してフ
ィルムのシールを施す。第6図も同様に、フィルム包装
された板付蒲鉾50の板51下面の両端の重ね合わせた小口
部分52に発振機に接続された振動子のホーン部分を接触
させてシールを施す。FIG. 5 is an explanatory diagram of an ultrasonic sealing device for sealing a center portion in FIG. 4, and FIG. 6 is an explanatory diagram of an ultrasonic sealing device for sealing a fore-edge portion in FIG. As shown in FIG. 5, the overlapping portion 42 at the center of the lower surface of the plate 41 of the film-wrapped plate 40 moving in the longitudinal direction is brought into contact with the rotated disk horn 43. The disk horn 43 transmits the ultrasonic waves from the vibrator 45 connected to the oscillator 44 to the film to seal the film. Similarly, in FIG. 6, the horn portion of the vibrator connected to the oscillator is brought into contact with the overlapped fore-edge portions 52 at both ends of the lower surface of the plate 51 of the film-wrapped plate 50 to seal.
第4図に示す2種類のシール方法を更に強くシールし
たものと、弱くシールしたものの4種類を使用してシュ
ードモナス菌を使用して変敗試験を行った。この場合、
強いシールとは手で強く引っ張っても溶着部分が剥がれ
ない状態を示し、弱いシールとは手で強く引っ張ると剥
がれる状態を示す。A deterioration test was carried out by using Pseudomonas bacteria using four types of the two types of sealing methods shown in FIG. 4 which were further strongly sealed and weakly sealed. in this case,
A strong seal indicates a state in which the welded portion is not peeled off even when strongly pulled by hand, and a weak seal indicates a state in which the welded portion is peeled off when strongly pulled by hand.
変敗試験の具体的な操作は前記実験例2〜4と同様
に、一つの項目で20サンプル(合計80サンプル)の蒲鉾
を容易し、各々菌を塗布後、菌塗布直後、10℃10日後、
20日後、30日後で5サンプルを抜きだし、シュードモナ
ス菌の有無をフキトリ検査で検出した。The specific operation of the deterioration test is similar to that of Experimental Examples 2 to 4 and facilitates the kamaboko of 20 samples (80 samples in total) in one item. ,
Twenty days and thirty days later, five samples were extracted, and the presence or absence of Pseudomonas was detected by a huttle test.
結果を次の第7表に示す。尚、表中aは製品外装(底
部のみ)、bは折込み部(外装内部)、cは製品表面
(外装内部)に残存する菌数の有無を示す。表中−は菌
の検出不能を、+は菌の検出確認を、++は菌の多量の
検出確認を示す。The results are shown in Table 7 below. In the table, a indicates the product exterior (only the bottom), b indicates the folded portion (inside the exterior), and c indicates the presence or absence of the number of bacteria remaining on the product surface (inside the exterior). In the table,-indicates no detection of bacteria, + indicates confirmation of detection of bacteria, and ++ indicates confirmation of detection of a large amount of bacteria.
第7表に示す通り、被験体Aでのシールはそのシール
の強さに関係なく検出されず、被験体Bでは逆にシール
の強さに関係なく検出された。これにより、シュードモ
ナス菌はシールの強さに関係なく、そのシール形態によ
り、侵入を防ぐことが確認された。 As shown in Table 7, the seal for the subject A was not detected irrespective of the strength of the seal, whereas the seal for the subject B was detected regardless of the strength of the seal. Thus, it was confirmed that the Pseudomonas bacteria were prevented from entering by the form of the seal regardless of the strength of the seal.
以上の結果より、変敗を起すシュードモナス菌は、前
記の通り60℃以上では死滅するため従来は製品が低温と
なるライン上で汚染されていた。このため、製品が前記
シュードモナス菌に汚染される前に、前記のような超音
波シール装置又はホットメルトシール装置のような、折
込み部分を密閉する処理を施す装置を敷設することによ
りライン上からの汚染を防止して、蒲鉾製品の変敗防止
が行えることが確認された。From the above results, Pseudomonas bacteria causing deterioration are killed at 60 ° C. or higher as described above, and thus, conventionally, the products were contaminated on a line where the temperature of the product was low. For this reason, before the product is contaminated by the Pseudomonas bacteria, by laying a device that performs a process of sealing the folded portion, such as an ultrasonic sealing device or a hot melt sealing device as described above, from the line, It was confirmed that contamination can be prevented, and that the Kamaboko product can be prevented from deteriorating.
具体的には前記第2図に示された工程のうち、[加
熱]工程の直前又は直後に本実験例の前記密閉装置を敷
設させて使用する。また、第2図に示す遠赤外線加熱装
置装置の直後に密閉装置を加えて敷設させてもよい。Specifically, of the steps shown in FIG. 2, the sealing device of the present experimental example is laid and used immediately before or immediately after the [heating] step. Further, a sealing device may be added and laid immediately after the far-infrared heating device shown in FIG.
実験例7(薬剤浸潤処理による変敗防止) 蒲鉾外装に付着したシュードモナス菌が折込み部分か
ら侵入することから、アルコール等の静菌剤を外装上か
ら塗布することで変敗防止可能かを検討した。Experimental Example 7 (Prevention of deterioration by chemical infiltration treatment) Since Pseudomonas bacteria adhering to the Kamaboko exterior penetrated from the folded part, it was examined whether application of a bacteriostatic agent such as alcohol from the exterior could prevent degradation. .
蒲鉾(商品名;小粋、(株)紀文製)製品に菌液(2.
3×105cells/ml)塗布後、同様に静菌剤を塗布し、定法
通り10℃に保存しNAC培地でシュードモナス菌の検査判
定した。Bacterial solution (2.
After applying 3 × 10 5 cells / ml), a bacteriostatic agent was applied in the same manner, stored at 10 ° C. as usual, and tested for Pseudomonas bacteria in NAC medium.
使用した薬剤は、有機酸系製剤であるMLS(商品名;
ミックルLS、日本新薬(株)製、使用濃度3%)、アル
コール系製剤であるSSH(商品名;サンサークルH、日
本新薬(株)製、使用濃度50%)、酵素系製剤であるF1
41(商品名;F141、日本新薬(株)製、使用濃度50%)
を使用した。The drug used was an organic acid-based preparation, MLS (trade name;
Mickle LS, Nippon Shinyaku Co., Ltd., use concentration 3%), alcohol-based drug SSH (trade name: Sun Circle H, Nippon Shinyaku Co., Ltd., use concentration 50%), enzyme preparation F1
41 (trade name; F141, manufactured by Nippon Shinyaku Co., Ltd., use concentration 50%)
It was used.
第8表に菌液塗布直後の(a)製品外装全面と(b)
折込部の残存コロニー数を示し、第9表に20日及び30日
間保存した時の残存コロニー数を示す。Table 8 shows (a) the entire product exterior and (b) immediately after application of the bacterial solution.
The number of remaining colonies in the fold is shown, and Table 9 shows the number of remaining colonies when stored for 20 days and 30 days.
第8表に示すように、菌を塗布した直後のフキトリ検
査でのシュードモナス菌のコロニー数は102オーダであ
り、直後でも折込み部(b)までシュードモナス菌が侵
入していた。 As shown in Table 8, the number of colonies Pseudomonas bacteria in wiping test immediately after the application of the bacteria is 10 2 order, Pseudomonas had penetrated to any flaps (b) immediately.
第9表に示す通り、無処理は10℃,20日目で折込み部
(b)までシュードモナス菌が達しており、菌数も>10
00であり全て黄緑色色素生産がされていた(変敗の初期
過程)、30日目では完全にシュードモナスにより軟化変
敗が起こっていた。一方、MLS,SSH,F141の各薬剤による
処理は完全に菌の侵入変敗を防止することが確認され
た。 As shown in Table 9, in the case of no treatment, Pseudomonas bacteria reached the fold (b) on day 20 at 10 ° C., and the number of bacteria was> 10.
It was 00 and all yellow-green pigments were produced (early stage of deterioration). On the 30th day, softening deterioration was completely caused by Pseudomonas. On the other hand, it was confirmed that treatment with each of MLS, SSH and F141 completely prevented invasion and deterioration of bacteria.
以上の結果より、変敗を起すシュードモナス菌は、本
実施例に示す薬剤により死滅することが確認された。こ
のため、前記シュードモナス菌に汚染される前(加熱処
理直後)に前記のような薬剤を浸潤させる装置を敷設す
ることによりライン上からの汚染を防止して、蒲鉾製品
の変敗防止が行えることが確認された。From the above results, it was confirmed that the Pseudomonas bacteria causing deterioration would be killed by the agent shown in this example. Therefore, by laying a device for infiltrating the above-mentioned chemicals before being contaminated by the Pseudomonas bacteria (immediately after the heat treatment), contamination from the line can be prevented, and deterioration of the Kamaboko product can be prevented. Was confirmed.
具体的には前記第2図に示された工程のうち、[加
熱]工程の直後に本実験例の薬剤を浸潤させる装置を敷
設させて使用する。また、第2図に示す遠赤外線加熱装
置装置の直後に本実験例の薬剤を浸潤させる装置を加え
て敷設させてもよい。Specifically, of the steps shown in FIG. 2, immediately after the [heating] step, a device for infiltrating the drug of this experimental example is laid and used. Further, a device for infiltrating the drug of the present experimental example may be added and laid immediately after the far-infrared heating device shown in FIG.
尚、本実施例中では、板付蒲鉾のうちリテーナ蒲鉾と
折込み蒲鉾について開示したが、加熱処理の後に包装し
て作る小田原蒲鉾についても、加熱処理の後の包装工程
が無菌的に行なわれるのならば、本願の問題としている
変敗の原因であるラインからのシュードモナス菌の汚染
をうけることとなり、本願の変敗防止処理を行なう対象
となるものである。In the present embodiment, the retainer kamaboko and the folding kamaboko are disclosed among the kamaboko with the plate, but also for the Odawara kamaboko packaged after the heat treatment, if the packaging process after the heat treatment is performed aseptically. In this case, Pseudomonas bacteria is contaminated from the line, which is the cause of the deterioration, which is the problem of the present application, and is subjected to the deterioration prevention processing of the present application.
[発明の効果] 本発明は以上説明したとおり、低温保管中における板
付蒲鉾を変敗(軟化型変敗)させる原因となる菌は、加
熱工程以降のライン上から板下面の折込み部分から侵入
する鞭毛又は線毛を有する走化性微生物の一つであるシ
ュードモナス菌(Pseudomonas sp.)であることを突き
止めた。[Effects of the Invention] As described above, in the present invention, bacteria causing deterioration (softening deterioration) of a plate-shaped kamaboko during low-temperature storage enter from the folded portion on the lower surface of the plate from the line after the heating step. It was identified as Pseudomonas sp., Which is one of the chemotactic microorganisms having flagella or fimbriae.
本第1及び第5発明ではこの鞭毛又は線毛を有する走
化性微生物の特性を考慮して、蒲鉾板下面の折込み部分
のみを対象として、該菌の侵入・繁殖を防止することに
より蒲鉾の軟化変敗を防止するため、板の上面に搭載さ
れている蒲鉾は、品質の低下を生じず、通常のライン上
に装置を敷設するのみでよい。In the first and fifth inventions, taking into account the characteristics of the chemotactic microorganisms having the flagella or fimbria, only the folded portion of the lower surface of the Kamaboko board is targeted to prevent the invasion and propagation of the fungus, thereby reducing In order to prevent softening and deterioration, the kamaboko mounted on the upper surface of the plate does not deteriorate in quality, and it is only necessary to lay the device on a normal line.
更に具体的に本第2〜第4及び第6〜第8発明では、
鞭毛又は線毛を有する走化性微生物の侵入・繁殖を防止
する方法及び装置が、板下面の折込み部分の加熱殺菌・
密封包装・薬剤静菌であるため、加熱薬剤等による蒲鉾
の変性、品質の低下がなく、装置も簡易なものとなる等
の効果がある。More specifically, in the second to fourth and sixth to eighth inventions,
A method and an apparatus for preventing the invasion and propagation of chemotactic microorganisms having flagella or fimbriae are disclosed.
Since it is hermetically sealed packaging and bacteriostatic, there are no effects such as denaturation of the kamaboko and deterioration of the quality due to the heating agent or the like, and the apparatus can be simplified.
第1a,b図は軟化再現試験操作を示す説明図、第2図は蒲
鉾製造装置から外装・梱包装置に至るライン上に敷設さ
れた遠赤外線加熱装置による変敗防止装置の一実施例を
示す概略図、第3a図,第3b図はシールの状態を示す説明
図、第4a図,第4b図は別のシールの状態を示す説明図、
第5図はセンター部分をシールする超音波シール装置の
説明図、第6図は小口部分をシールする超音波シール装
置の説明図、第7a図,第7b図は蒲鉾製品の下斜め方向か
らの斜視図である。FIGS. 1a and 1b are explanatory diagrams showing a softening reproduction test operation, and FIG. 2 shows an embodiment of a deterioration prevention device using a far-infrared heating device laid on a line from a kamaboko manufacturing device to an outer packaging device. Schematic diagrams, FIGS. 3a and 3b are explanatory diagrams showing states of seals, FIGS. 4a and 4b are explanatory diagrams showing states of other seals,
Fig. 5 is an explanatory diagram of an ultrasonic sealing device for sealing a center portion, Fig. 6 is an explanatory diagram of an ultrasonic sealing device for sealing a fore edge portion, and Figs. It is a perspective view.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 南波 護 神奈川県横浜市戸塚区上倉田町346 メ ゾン戸塚A―102 (72)発明者 中原 省吾 神奈川県横浜市金沢区金沢町184―111 (72)発明者 西浦 康雄 大阪府三島郡島本町桜井4丁目20番11号 (56)参考文献 特開 昭48−103768(JP,A) 特開 昭51−44091(JP,A) 特開 昭59−196030(JP,A) ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Nanba Mamoru 346, Kamikuratacho, Totsuka-ku, Yokohama-shi, Kanagawa Prefecture Maison Totsuka A-102 (72) Inventor Shogo Nakahara 184-111, Kanazawacho, Kanazawa-ku, Yokohama-shi, Kanagawa Prefecture (72) ) Inventor Yasuo Nishiura 4-20-11 Sakurai, Shimamoto-cho, Mishima-gun, Osaka (56) References JP-A-48-103768 (JP, A) JP-A-51-44091 (JP, A) JP-A-59- 196030 (JP, A)
Claims (8)
し、折り込み部分が前記板の下面上になるように全体を
実質的に無菌状態で包装してなる板付蒲鉾の製造方法に
おいて、 包装後に、前記折り込み部分のある板下面部のみに、鞭
毛又は線毛を有する走化性微生物の前記折り込み部分へ
の侵入を阻止する処理を施すことを特徴とする板付蒲鉾
の製造方法。1. A method for producing a kamaboko with a plate, wherein a surimi such as fish meat is placed on the upper surface of the plate and cooked, and the whole is packaged in a substantially aseptic state so that the folded portion is on the lower surface of the plate. A method for producing a kamaboko with a board, comprising, after packaging, a treatment for preventing invasion of chemotactic microorganisms having flagella or fimbria into the folded section only on the lower surface of the board having the folded section.
において、 前記侵入を阻止する処理が、前記折り込み部分のある板
下面を60℃以上に加熱する加熱処理であることを特徴と
する板付蒲鉾の製造方法。2. The method for manufacturing a kamaboko with a plate according to claim 1, wherein the treatment for preventing the invasion is a heating treatment for heating the lower surface of the plate with the folded portion to 60 ° C. or more. A method of manufacturing Kamaboko with a plate.
において、 前記侵入を阻止する処理が、前記板下面の折り込んで重
ね合わせられた部分を小口方向と長手方向とをシールす
る前記走化性微生物の侵入経路を塞ぐ処理であることを
特徴とする板付蒲鉾の製造方法。3. The method for manufacturing a kamaboko with a plate according to claim 1, wherein the step of preventing the intrusion includes the step of sealing the folded and overlapped portion of the lower surface of the plate in the fore-edge direction and the longitudinal direction. A method for producing a kamaboko with a plate, which is a treatment for closing a path for invasion of a activating microorganism.
において、 前記侵入を阻止する処理が、前記折り込み部分のある板
下面の外装上からの静菌剤の浸潤による前記走化性微生
物の静菌処理であることを特徴とする板付蒲鉾の製造方
法。4. The method for producing a kamaboko with a plate according to claim 1, wherein the treatment for preventing invasion is performed by infiltration of a bacteriostatic agent from the exterior of the lower surface of the plate with the folded portion. A method for producing a kamaboko with a plate, which is a bacteriostatic treatment.
し、折り込み部分が前記板の下面上になるように全体を
実質的に無菌状態で包装してなる板付蒲鉾の製造装置に
おいて、 包装後に、前記折り込み部分のある板下面部のみに、鞭
毛または線毛を有する走化性微生物の前記折り込み部分
への侵入を阻止する処理を施す装置を備えたことを特徴
とする板付蒲鉾の製造装置。5. An apparatus for manufacturing a kamaboko with a plate, wherein a surimi such as fish meat is placed on the upper surface of the plate and cooked, and the whole is packaged in a substantially aseptic state so that the folded portion is on the lower surface of the plate. After packaging, only the lower surface of the plate with the folded portion is provided with a device for performing a treatment for preventing chemotactic microorganisms having flagella or fimbria from entering the folded portion, the production of a kamaboko with a plate apparatus.
において、 前記侵入を阻止する処理を施す装置が、前記折り込み部
分のある板下面を60℃以上に加熱する加熱装置であるこ
とを特徴とする板付蒲鉾の製造装置。6. The apparatus for manufacturing a kamaboko with a plate according to claim 5, wherein the device for performing the treatment for preventing the invasion is a heating device for heating the lower surface of the plate with the folded portion to 60 ° C. or higher. Characteristic kamaboko manufacturing equipment.
において、 前記侵入を阻止する処理を施す装置が、前記板下面の折
り込んで重ね合わせられた部分を小口方向と長手方向と
をシールする密閉装置であることを特徴とする板付蒲鉾
の製造装置。7. The apparatus for manufacturing a kamaboko with a plate according to claim 5, wherein the device for performing the treatment for preventing the invasion seals the folded and overlapped portion of the lower surface of the plate in the fore-edge direction and the longitudinal direction. An apparatus for producing a kamaboko with a plate, characterized in that the apparatus is a closed apparatus.
において、 前記侵入を阻止する処理を施す装置が、静菌剤を前記折
り込み部分のある板下面の外装上から浸潤させる装置で
あることを特徴とする板付蒲鉾の製造装置。8. The apparatus for manufacturing a kamaboko with a plate according to claim 5, wherein the device for performing the treatment for preventing the invasion is a device for infiltrating the bacteriostatic agent from the exterior of the lower surface of the plate with the folded portion. An apparatus for manufacturing a Kamaboko with a plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1124519A JP2603537B2 (en) | 1989-05-19 | 1989-05-19 | Manufacturing method and apparatus for kamaboko with plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1124519A JP2603537B2 (en) | 1989-05-19 | 1989-05-19 | Manufacturing method and apparatus for kamaboko with plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02303464A JPH02303464A (en) | 1990-12-17 |
| JP2603537B2 true JP2603537B2 (en) | 1997-04-23 |
Family
ID=14887491
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1124519A Expired - Fee Related JP2603537B2 (en) | 1989-05-19 | 1989-05-19 | Manufacturing method and apparatus for kamaboko with plate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2603537B2 (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS48103768A (en) * | 1972-04-12 | 1973-12-26 | ||
| JPS5753046B2 (en) * | 1974-10-09 | 1982-11-11 | ||
| JPS59196030A (en) * | 1983-04-23 | 1984-11-07 | Mitsubishi Gas Chem Co Inc | Preservation of fish paste product |
-
1989
- 1989-05-19 JP JP1124519A patent/JP2603537B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02303464A (en) | 1990-12-17 |
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