JP2653721B2 - Plastic slide for optical microscope - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a slide and a cover used for holding a sample when observing the sample with an optical microscope. More specifically, the slide and the cover are made of plastic and integrated. This is mainly used for observing liquid samples.

[Conventional technology]

Conventionally, glass was used for a slide and a cover for holding a sample when using an optical microscope. The surface of the slide and cover was almost planar because it was quite difficult to machine the glass surface to a shape other than planar. Therefore, a sample is dropped on a slide, a cover glass is placed on the slide, and the sample is observed with a microscope.Since the thickness of the liquid phase of the sample slightly changes each time, complicated operations are required to keep this constant. Met. In addition, since the sample adhered to the periphery of the cover glass, it was difficult to avoid that the sample adhered to the fingertip.

In recent years, with the development of plastic, plastic slides and covers have been made. A slide and cover integrally molded are also commercially available. Although the thickness of the liquid phase is constant due to the integral molding, the thickness is very thick at 80 to 100 μm, which is inappropriate for high-magnification observations, and the liquid easily leaks to the outside. It is a structure that adheres to In addition, although a scale line is formed on the outer surface of the cover, the scale line cannot be observed together with the sample, as is clear from the relationship with the depth of focus described later. Currently, plastic slides and covers are used for medical institutions and other inspections.
It is mainly used for preliminary inspection, and there is no such thing that can be used for detailed inspection. Further, Japanese Utility Model Laid-Open No. 2-2711 discloses a slide in which a narrow groove is cut in a square shape on a plastic slide, and a portion surrounded by the groove is a portion on which a sample is placed and observed. ing. The plastic cover is large enough to cover the entire area surrounded by the square groove and the surrounding groove, and can be moved in parallel to the slide surface by a guide provided on the slide surface.

When the cover is moved to cover the sample dropped on the square portion of the slide, a liquid phase portion is formed between the cover and the slide, and the excess sample falls into a groove around the square.

Although a liquid phase having a constant thickness can be formed by this method, it is necessary to move the cover with a fingertip, and there is a disadvantage that the sample easily adheres to the fingertip.

[Problems to be solved by the invention]

When observing a liquid sample with an optical microscope, usually, a droplet dropped onto a slide glass is covered with a cover glass and observed. However, it is difficult to make the thickness of the liquid phase constant with this method. The cells in the sample may be destroyed because the cover glass is pressed too much. When counting a specific object in a sample, the thickness of the liquid phase needs to be constant.

However, it is quite complicated to keep the thickness of the liquid phase constant, and it is difficult to avoid that the sample adheres to the fingertip by such an operation. Therefore, there is a great demand for a method in which the thickness of the liquid phase becomes constant with a simple operation, and the sample does not adhere to the fingertip at all.

Particularly in recent medical examinations, hepatitis and other harmful viruses may be mixed into the sample, so it is important to prevent the sample leaking from the slide glass from adhering to the examiner or other human body. It has become a challenge.

In consideration of these points, the present invention can make the thickness of the liquid phase of a sample constant by an extremely simple method, and leaks out when the sample is set on a slide glass or during subsequent handling. An object of the present invention is to provide a slide and a cover for an optical microscope, which are not likely to be attached to a human body directly. If necessary, a scale is provided on the slide to facilitate counting of the specific object so that the object and the scale can be observed simultaneously. In the following description, the plastic slide for the optical microscope and the cover are collectively referred to as a plastic slide for convenience.

[Means for solving the problem]

The present inventors have paid attention to the fact that by changing the slide and cover from conventional glass to plastic, it is possible to impart a fine shape to the slide, which was difficult with glass, and the form and function that can be imparted to the slide or cover As a result of intensive studies on the relationship, the present invention has been reached.

That is, (1) In a structure in which the sample holding cell of the optical microscope is formed of a slide, a cover, and a partition, the slide and the cover are made of a transparent plastic plate. It has the function of keeping the interval constant, has an opening for sample injection at the front of the cell, and has a small gas flow port at the rear of the cell that allows gas to flow but liquid cannot pass through. In a structure in which a plastic slide for an optical microscope and (2) a slide for holding a sample of an optical microscope, a cover, and a partition are made of plastic and a cell for holding a sample is formed inside the slide, a front and a back of the cover. The length in the direction is shorter than the slide, the left and right sides of the cell are closed by a partition between the slide and the cover, and slide along the front and rear edges of the cover The gap between the front groove of the slide and the cover serves as a sample injection port, the gap between the rear groove of the slide and the cover serves as a gas flow port, and is defined by the left and right partitions and the front and rear grooves. (3) In the above structure, the length of the cover in the front-rear direction is smaller than that of the slide, and the left and right sides of the cell are the slide and the cover. The space is closed by a partition, and a portion having a taper in which the gap between the slide and the cover gradually increases toward the outside is provided at the front and rear portions of the slide,
A groove is provided on the slide along the front and rear edges of the cover outside the tapered portion, the gap between the slide and the tip of the cover serves as a gas flow port, and is partitioned by the left and right partitions and the front and rear tapered portions. A sample holding cell having a predetermined interval, a sample injection port leading to a front groove, and a front and rear edge of a slide serving as a weir. It is a slide.

Further, a plastic slide having a ridge-shaped convex portion provided on the cover side at least along the edge of the rear portion of the cover to form a cell, and a sample injection port at the front portion of the cover and a gas flow port at the ridge-shaped convex portion at the rear portion. It is. Alternatively, a convex scale line can be provided on the inner surface side of the slide cell.

 Hereinafter, the present invention will be described in detail.

In the present invention, the slide and cover for holding the sample of the optical microscope need to be made of plastic. Furthermore, in the inventions set forth in claims 2 and 3 below, the partition walls of the cells need to be made of plastic. The type of plastic is not particularly limited. A wide range of materials are available. For example, as thermoplastics, polymethyl methacrylate resin, polystyrene resin, polycarbonate resin, polysulfone resin, polyester resin, nylon resin, ethylene acrylic resin, polyethylene resin, polypropylene resin, poly 4-methylpentene-1 resin, polyvinyl chloride The thermosetting plastic is a phenol resin, a furan resin, or the like, and these may be appropriately mixed and used.

Since some of these plastics contain a plasticizer, it is necessary to select them in consideration of the properties of the sample. In the case of heat sterilization, heat resistance must also be considered. For example, when it is necessary to perform a sterilization treatment, polycarbonate, polysulfone, poly-4-methylpentene-1
Plastics with high heat resistance such as are suitable.

The plastic used for the slide and cover is preferably highly transparent and also has high optical isotropy, particularly 10
This is important when observing at a high magnification of 00 or more. In general, many thermoplastics have crystallinity and exhibit birefringence upon stretching. Therefore, care must be taken so that birefringence is not generated during molding.

In recent years, plastic lenses have been developed, and plastics having high transparency and optical isotropy have been developed. Further, plastics having higher light transmittance by surface coating treatment have been used.

Recently, acrylic resins have been mainly used as materials for these optical plastics. These are extremely excellent as the plastic raw material of the present invention.

Also, the slide and the cover may be the same plastic material or different materials.

The material of the partition wall is not particularly limited. However, it is preferable that the distance between the slide and the cover is easily maintained constant if the partition is integrally formed with the slide or the cover using plastic. Also, an adhesive strip or an adhesive can be used as the partition wall. The shape and structure of the sample inlet above the cell are not particularly limited. The sample is mainly a liquid, but a wide range can be used as long as the cell can be filled.

In the lower part of the cell, a minute gas flow port through which gas flows but liquid cannot pass is provided. The plastic slide etc. of the present invention is mainly used for liquid microscopy,
Most commonly used are blood, cells, urine, etc., at medical institutions, which are diluted with a medium used in the medical field such as water, physiological saline, ethylene glycol, dimethyl sulfoxide, and the like. When the pore size of the circulation port is 5 μm, air flows, but those solutions cannot pass through, and even when the pore size reaches 10 several μm, the solution hardly passes. Solution and slide hydrophilicity,
Depending on the relationship between the adhesion and cohesion depending on the degree of hydrophobicity, and other physical properties such as the viscosity of the solution, there is a case where almost no leakage occurs even when the thickness becomes about 1 mm. However, it is preferably at most 500 μm, more preferably at most 100 μm. Therefore, when the sample is injected into the cell, the air inside the cell is discharged to the outside through the gas flow port and the inside of the cell is replaced with the sample, but the sample is prevented from leaking to the outside. Further, the flow port may have a shape such as a slit or a groove or a micropore structure.

In recent medical examinations, prevention of harmful viruses such as hepatitis has become an important issue.

Next, the structure of the plastic slide of the present invention will be described with reference to the drawings.

FIG. 1 is a plan view of one embodiment of a plastic slide or the like described in claim 2, and FIG.
3 is an enlarged cross-sectional view taken along line BB of FIG. The width of the cover 2 is smaller than the width before and after the slide 1 (FIG. 3), and there is a cell 5 (FIG. 2) formed between the slide and the cover, into which a sample is injected. The left and right sides of the cell are closed by partition walls 3. In the belt-like portion 5 between the partition walls, the distance between the slide and the cover is kept constant, and the transmitted light in this portion is observed with a microscope. The thickness of this part, that is, the thickness of the liquid phase of the cell is preferably 10 to 20 μm in consideration of the depth of focus and attenuation of transmitted light when observing at a high magnification of several hundred times or more. When observing blood, urine, cells, etc., which are frequently used in institutions at a magnification of 200 to 400, a thickness of about 40 to 50 μm is appropriate. Also, the area of the observation part is appropriately selected depending on the purpose of use,
Most are about 18 × 18mm.

In addition, the thickness of the slide and cover are closely related to the performance of the condenser and objective lens of the microscope.
Specified, the range is 0.5 to 1.5 mm for slides, cover is
0.06 to 0.25 mm. These are selected according to the conditions of use, but the most frequently used cover thickness is JIS No. 1-
It is 0.15-0.18 mm in S.

Grooves 4 and 6 are provided on the slide along the front and rear edges of the cover 2, respectively. When the sample is injected into the groove 4, the cell 5 is instantaneously filled by capillary action, and the surplus is accumulated in the grooves 4 and 6, and the sample is in a state shown by a set of many points in FIG. . When the sample is accumulated in the groove,
Even if the sample evaporates a little, it is replenished to the cell 5 from the groove by capillary action, and no air bubbles are generated inside the cell. Therefore, a sample can be prepared extremely easily by using the plastic slide or the like of the present invention. In addition, 7 is a narrow slit, which has the function of the aforementioned minute vent,
When the sample is injected, the internal air is released from this, but even if the liquid fills the groove or the slide is tilted, the sample hardly leaks out. According to the test results, in the case of an aqueous sample, the interval is suitably about 10 to 20 μm. When the sample is injected into the cell, the sample is in a state shown by a set of many points in FIG. Since the thickness of the liquid phase in the cell 5 is narrow, the sample is held with a strong force by capillary action in this region. Generally, when observing at a high magnification, the force of holding the liquid is further increased by capillary action (adhesion of the liquid to the slide and the cover) to thin the sample (liquid phase), and the distance between the slide 1 and the cover 2 is reduced. Such power works. For precise observation at a higher magnification, a thin cover is required due to the design of the optical system of the microscope. The specific thickness of the cover varies slightly depending on the microscope and the manufacturer, but is often specified as 0.17 mm at 1000 times. With this thickness, even a glass cover is distorted to some extent on the slide side, and the liquid phase becomes thinner at the center of the cell, but more so with plastic. This will be described later on the scale line of the slide.

When a plastic slide in which a sample has been injected into a cell is stored as a preparation, a preparation can be easily prepared by pouring molten paraffin into the grooves 4 and 6.

When a microscope is used, the sample inside the cell and the light transmitted through the slide and the cover are magnified and observed by the optical system of the microscope. Therefore, this part of the slide and the cover requires high optical isotropy and flatness. However, at present, molding techniques comparable to glass have been obtained due to advances in mold manufacturing techniques and plastic molding techniques.

Although the plastic slide of the present invention is made as one piece, it is not possible to perform so-called integral molding, as is clear from the cross sections shown in FIGS. Therefore, after molding the slide part and the cover part separately,
For example, it is necessary to integrally form the space between the partition wall 3 and the cover 2 or the space between the partition wall 3 and the slide 1 by a method such as fusing ultrasonic waves or bonding with a plastic adhesive.
In the present invention, "made in one piece" means such a structure.

When actually making a plastic slide, the cover cuts a wide sheet into a rectangle, and the other parts (slide and partition) are integrally molded with a mold and fused or bonded between the cover 2 and the partition 3. It is most efficient to do so.

FIG. 4 is a plan view of one embodiment of the plastic slide described in claim 3, FIG. 5 is a cross-sectional view taken along the line CC, and FIGS. E-
FIG. 3 is an enlarged cross-sectional view of E. The structures of the slide 1, the cover 2, the partition 3, the cell 5, and the groove 6 shown in these figures are the same as those described in FIGS. 1 to 3 above. The description regarding the area of the observation part of the cell, the thickness of the slide and the cover, the function of the sample being instantaneously filled in the cell due to the capillary phenomenon, the operation of the slit 7, and the description of the method of preparing the plastic slide also relate to FIGS. Exactly as described.

However, in the plastic slide shown in FIG. 4, a tapered portion 8 is provided between the cell 5 and the grooves 4 and 6 so that the distance between the slide and the cover gradually increases toward the outside.
Here, if the shape of the tapered portion is such that the liquid can be sucked into the center of the cell by capillary action, for example,
It may be shaped like a wrapper or other shapes.
Slits 9 and 7 are provided between the slide and the front and rear edges of the cover, respectively. This is because air flows but liquid does not pass. Further, a sample injection port 10 communicating with the groove 4 is provided. Further, the front and rear edges of the slide 1 are weirs 13.

When the sample is injected into the sample inlet 10, the sample enters the groove 4 and is instantaneously filled in the cell 5 by capillary action. At this time, the air inside the cell is discharged from the slit 7 to the outside. If the injection amount of the sample 14 is appropriate, the sample 14 is held inside the cell 5 in a state indicated by a set of many points in FIG. As is clear from this figure, since the liquid is held so as to be sucked into the center of the cell, when a small amount of the sample evaporates, the cell 5 is removed from the tapered portion 8 by capillary action.
Since the sample is supplied to the cell, no bubbles are generated inside the cell. Even if the injection amount of the sample is slightly larger or smaller, it does not leak because the cell 5 and the tapered portion 8 are held by the capillary action, and does not leak out even when the slide is tilted. This is one of the most important parts of the present invention.

Furthermore, for some reason, the sample leaks to the outside and the slit 7
Also, when it adheres between the weir 13 and the weir 13, it does not spill outside due to the weir 13. Therefore, if the person handling the sample holds the outside of the weir 13, the sample does not directly contact the body. In recent medical examinations, prevention of harmful viruses such as hepatitis has become an important issue. By using the plastic slide of the present invention, even in such a test, it is possible to prevent virus infection by a very simple method. This is one of the advantages of the present invention.

Further, when the sample injection amount is large and accumulated in the grooves 4 and 6, if only the sample injection port is closed, only the slits 7 and 9 are in communication with the outside air. Does not leak.

The slide of the present invention can be provided with a convex scale line on the cell side surface of the slide 1. The convex cross section of the graduation line and the shape of the ridge line may have any shape. In order that the image of the graduation line can be observed sharply, a shape in which the cross section at the top is an acute angle and the height is constant, that is, a triangular pyramid shape is most preferable. The scale line may be provided only on a part of the cell or on the entire surface. The arrangement of the scale lines is preferably the lattice shape shown in FIG. 8 or the parallel lines shown in FIG. 9, but may be other shapes. In addition, when there is a possibility that the cell membrane or the like of the sample may be damaged by the convex portion having a sharp cross section at the top, it is necessary to caulk the top.

It is also necessary to consider that the depth of focus of the microscope is very shallow. Specifically, about 5 to 8 μm at 300 times and about 700 at 700 times
At 1.5 to 2.5 μm and 1000 times, it is reduced to about 0.9 to 1 μm and 1 μm or less. In other words, since only a very narrow range is visible, the scale line is observed as a sharp straight line only when the focus is on the top of the scale line.

Even when the shape of the graduation line is a triangular pyramid as described above, when the focal point is in the middle, two parallel lines are formed. When the focal point is above the top, the graduation line cannot be observed. Scale line height is one-third of cell thickness under normal operating conditions
Since it is considered to be to the extent, more than half is a region where the scale line cannot be observed. Therefore, as shown in Fig. 10, if a conical projection is provided on a part of the scale line and its height is made to correspond to the distance between the slide and the cover, the scale can be observed even when focusing on any part of the cell. . For example, when the focus is near the slide surface, it is observed as shown in FIG. 11 (a) in the field of view of the microscope, and when the focus is near the cover, it is observed as shown in FIG. 11 (b). You. Therefore, even if the focus is moved up and down to observe any portion of the sample, the sample and the clear scale can be observed simultaneously.

Some conventional plastic slides are integrally molded and have a scale provided outside the cell, but the scale cannot be observed at all with a microscope. This is obvious when considering the relationship between the depth of focus and the position of the scale. In the present invention, since the scale line is provided on the inner surface of the cell, not only can it be clearly observed with the sample, but also by making the scale line into a shape as shown in FIG. The sample can be observed simultaneously.

In the description of FIG. 3, when the sample is injected into the cell, a force acts to reduce the gap between the slide and the cover due to capillary action, so that the cover is bent toward the cell side, and the degree of the plastic cover is more than that of glass. Said big. If the height of the conical protrusion of the graduation line in FIG. 10 is made equal to the thickness of the cell and the top is caulked to prevent the cover from bending, the slide and the cover can be always kept parallel.

It is not practical to provide a ridge-shaped protruding portion on the inner surface of the cell because it is very difficult and expensive for glass. Although it is relatively easy to cut a groove-shaped graduation line on glass, it is as described above that the graduation cannot be used effectively due to the depth of focus. However, once plastics are processed in such a manner, it is easy to reproduce a large number of molded products. Therefore, a slide provided with a scale line having a complicated shape as shown in FIG. 10 can be said to be a structure that makes the best use of the characteristics of plastic.

13 and 14 are convex, have a triangular cross section, and have a line interval.
Using a slide with orthogonal graduation lines of 0.3 mm, 19 μm width and 11 μm height, dilute blood with saline, photograph red blood cells under a microscope, double the magnification, 200 and 3
It is a model figure of the photograph magnified 20 times, 11 shows a scale line and 17 shows red blood cells. From this, it is recognized that both the red blood cells and the scale line can be clearly observed, and the counting of the red blood cells and the like become easy. Further, since the height of the graduation line is 11 μm, the graduation line can be clearly observed even when the focus is moved and observed.

Further, in the present invention, a colored plastic can be used. Observation through a filter is often necessary depending on the nature of the sample and the purpose of observation. For example, a green filter is often used for observing cells.
The use of pre-colored plastic slides eliminates the need for filters.

Although it is possible to color a glass slide so as to have the same function as a filter, a colored slide is rarely used because it is very expensive.
However, coloring of plastic is extremely easy and inexpensive.

In the plastic slide of the present invention, one cell can be provided on one slide, or two or more cells can be provided. Recently, a large number of microscopic examinations are performed in medical institutions and the like, and thus, if a large number of cells are provided on one slide, the examination efficiency can be greatly improved.

In the aspect described in claim 4, it is necessary to provide a ridge-shaped convex portion at least at the rear portion in order to seal a gap between the slide and the cover. This projection may be provided on the cover side or on the slide side. In this embodiment, contrary to the embodiments described in claims 2 and 3, the structure on the slide side is minimized, and many structures are provided on the cover side. The partition walls and the ridge-shaped convex portions are in close contact with each other, so that the sample does not leak. Further, the ridge-shaped convex portion can be provided not only at the rear portion but also at the front portion.

FIG. 15 is a plan view of a mode in which ridge-shaped protrusions are provided on the front and rear portions of the slide, and FIG. 16 is a cross-sectional view taken along the line A-A. The figure is an enlarged sectional view of BB and CC. The intersection of the partition wall 3 and the ridge-shaped convex portion 15 may be fitted by making a cutout in one of them, or the ridge-shaped convex portion may have a length corresponding to the interval between the partition walls, or a plastic material may be used for one of them. Made to deform and seal when the slide and cover are combined, a wide range of materials suitable for this purpose can be used. A notch is formed in the ridge-shaped convex portion between the sample inlet 10 and the cell 5, and the sample is injected into the cell. Further, a minute gas flow port 16 is provided in the rear ridge-shaped convex portion, and when the sample is injected into the cell, the internal air is released, but the sample does not leak out. The specific size and function of the gap between the distribution ports are as described above.

〔The invention's effect〕

By using the plastic slide of the present invention, the thickness of the sample (liquid phase) to be observed can be made constant by a very simple operation. Moreover, since the sample is held between the slide and the cover by the surface tension, there is no possibility that the sample leaks outside.

Recently, a large number of microscopic examinations are performed daily in medical institutions, and at that time, prevention of infection with hepatitis virus or the like has become an important issue. The slide of the present invention has no risk that the leaked sample will directly adhere to the examiner or other human body, and since it is made of plastic, the sample containing dangerous substances can be incinerated together with the slide after use. Therefore, it is particularly suitable for such applications. This is even more so in consideration of the fact that a fingertip was often damaged with a cover glass while using a microscope.

Also, by providing a scale line with a convex triangular cross section on the cell side surface of the slide and a conical protrusion as shown in Fig. 10 on the ridge line, the sample and the scale can be adjusted regardless of the focus. It can be observed at the same time.

Furthermore, by coloring plastic slides,
By eliminating the need for a filter and providing a large number of cells on one slide, the testing efficiency can be greatly improved.

[Brief description of the drawings]

FIG. 1 is a plan view of one embodiment of the plastic slide of the present invention, FIG. 2 is a cross-sectional view taken along AA, and FIG. 3 is BB.
1 is an enlarged cross-sectional view of FIG. FIG. 4 is a plan view of one embodiment of the plastic slide of the present invention, and FIG. 5 is a cross-sectional view taken along the line CC of FIGS.
The figures are enlarged cross-sectional views taken along DD and EE, respectively. 8 and 9 show plan views of graduation lines provided on the cell side surface of the slide. FIG. 10 shows a front view and a plan view of a part of one scale line. FIG. 11 (a) shows that when the scale line in FIG. 8 has a shape as shown in FIG. 10, a portion close to the slide surface of the cell (focus on the ridge of the convex part of the scale line) is observed with a microscope. FIG. 11 (b) shows an image of the field of view observed, and FIG. 11 (b) shows an image of the field of view near the cover. FIG. 12 is a plan view of a slide or the like when a large number of cells are provided on one slide. FIGS. 13 and 14 use a slide having a scale line having a convex and triangular cross section, photographing a red blood cell of blood diluted with physiological saline with a microscope and stretching it by a factor of 200.
It is a model figure of the photograph of the red blood cell and the scale line which are 320 times and. FIG. 15 is a plan view of one embodiment of a plastic slide or the like described in claim 4, FIG. 16 is a cross-sectional view taken along the line AA, and FIG. 17 and FIG. FIG. 4 shows an enlarged cross-sectional view of CC. DESCRIPTION OF SYMBOLS 1 ... Plastic slide 2 ... Plastic cover 3 ... Cell partition 4, 6 ... Cell groove 5 ... Cell 7, 9 ... Slit between slide and cover 8 ... Taper between slide and cover Part 10: Sample inlet 11: Scale line 12: Image of the scale line seen by microscope 13 ... Weir 14: Sample 15: Ridge-shaped protrusion 16: Gas flow port 17: Red blood cells

Claims (5)

(57) [Claims] 1. A sample holding cell of an optical microscope is a slide,
In the structure formed by the cover and the partition, the slide and the cover are made of a transparent plastic plate, and the partition has a function of sealing the left and right sides of the cell and maintaining a constant gap between the slide and the cover. A plastic slide for an optical microscope, characterized in that the plastic slide has a fine gas flow port through which gas flows but liquid cannot pass through the opening for sample injection and the rear of the cell. 2. A structure in which a slide, a cover and a partition for holding a sample of an optical microscope are made of a single piece of plastic, and a cell for holding a sample is formed inside thereof.
The length of the cover in the front-rear direction is shorter than the slide, the left and right sides of the cell are closed by a partition between the slide and the cover, and a groove is provided on the slide along the front and rear edges of the cover,
The gap between the groove at the front of the slide and the cover serves as a sample injection port, the gap between the groove at the back of the slide and the cover serves as a gas flow port, and a sample having a predetermined space defined by the left and right partitions and the front and rear grooves. A plastic slide for an optical microscope, comprising a holding cell. 3. A structure in which a slide, a cover, and a partition for holding a sample of an optical microscope are made of a single piece of plastic, and a cell for holding the sample is formed therein.
The length of the cover in the front-rear direction is shorter than the slide, and the left and right sides of the cell are closed by a partition between the slide and the cover, and the front and rear sides of the slide have a taper that gradually widens the gap between the slide and the cover. A portion is provided, a groove is provided on the slide along the edges of the front and rear portions of the cover outside the tapered portion, the gap between the slide and the tip of the cover serves as a gas flow port, and the left and right partitions and the front and rear The tapered portion forms a sample holding cell with a predetermined interval, and has a sample inlet that communicates with the front groove, and the front and rear edges of the slide are weirs. Plastic slide for optical microscope. 4. A structure in which a slide, a cover and a partition for holding a sample of an optical microscope are made of one piece of plastic, and a cell for holding a sample is formed in the inside thereof.
The length of the cover in the front-rear direction is shorter than the slide, the left and right of the cell are closed by a partition between the slide and the cover, and at least the rear portion of the cover is provided with a ridge-shaped convex portion that seals a gap with the slide. Between the partition walls and the ridge-shaped protrusions are sealed,
The left and right partition walls and the ridge-shaped protrusions form a sample holding cell having a predetermined interval, and a sample inlet is provided at the front of the cover, and gas flows through the rear ridge-shaped protrusion. A plastic slide for an optical microscope, characterized in that it has a minute gas flow port through which a liquid cannot pass. 5. The slide according to claim 1, wherein a convex scale line is provided on the cell side surface of the slide.
A plastic slide for an optical microscope according to the above item.