JP2674674B2 - Removal method of nitrate nitrogen in roughage - Google Patents
Removal method of nitrate nitrogen in roughageInfo
- Publication number
- JP2674674B2 JP2674674B2 JP2338615A JP33861590A JP2674674B2 JP 2674674 B2 JP2674674 B2 JP 2674674B2 JP 2338615 A JP2338615 A JP 2338615A JP 33861590 A JP33861590 A JP 33861590A JP 2674674 B2 JP2674674 B2 JP 2674674B2
- Authority
- JP
- Japan
- Prior art keywords
- roughage
- nitrate nitrogen
- nitrate
- yeast
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 title claims description 19
- 235000013325 dietary fiber Nutrition 0.000 title claims description 13
- 238000000034 method Methods 0.000 title claims description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- 244000286779 Hansenula anomala Species 0.000 claims description 17
- 241000894006 Bacteria Species 0.000 claims description 9
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 9
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 5
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 235000014683 Hansenula anomala Nutrition 0.000 claims description 2
- 108090000913 Nitrate Reductases Proteins 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 description 9
- 239000000126 substance Substances 0.000 description 6
- 208000005374 Poisoning Diseases 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 231100000572 poisoning Toxicity 0.000 description 5
- 230000000607 poisoning effect Effects 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 229910002651 NO3 Inorganic materials 0.000 description 4
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 4
- 230000000711 cancerogenic effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 244000138286 Sorghum saccharatum Species 0.000 description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 3
- 231100000315 carcinogenic Toxicity 0.000 description 3
- 210000003850 cellular structure Anatomy 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 239000004460 silage Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 231100000357 carcinogen Toxicity 0.000 description 2
- 239000003183 carcinogenic agent Substances 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000006872 mrs medium Substances 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical compound ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- General Preparation And Processing Of Foods (AREA)
- Fodder In General (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、粗飼料に含有され、家畜や人がこれを摂取
すると体内で他の物質と化合して中毒症状を呈したり、
また、発ガン性物質を生成したりする硝酸態窒素を除去
するようにした粗飼料における硝酸態窒素の除去方法に
関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial field of use] The present invention is contained in roughage, and when ingested by livestock and humans, it combines with other substances in the body to exhibit poisoning symptoms,
The present invention also relates to a method for removing nitrate nitrogen in a roughage which is adapted to remove nitrate nitrogen that produces a carcinogen.
従来、牧草のような粗飼料中には、硝酸，硝酸塩等の
硝酸態窒素を含有していることが知られている。そし
て、これらを家畜や人が摂取すると体内で亜硝酸となっ
て他の物質と化合して硝酸塩中毒症状を起こしたり、ま
た、発ガン性のニトロソアミン物質を生成したりするこ
とも知られている。Conventionally, it has been known that roughage such as grass contains nitric nitrogen such as nitric acid and nitrate. It is also known that when these are ingested by livestock and humans, they become nitrite in the body and combine with other substances to cause nitrate poisoning symptoms, or to generate carcinogenic nitrosamine substances. .
ところが、上記のように家畜や人が摂取すると体内で
亜硝酸となり、他の物質と化合して中毒症状を起こした
り、また、発ガン性物質を生成したりする元凶となる硝
酸態窒素を、粗飼料から積極的に除去する技術は開発さ
れていないのが現状である。However, as mentioned above, when ingested by livestock and humans, it becomes nitrous acid in the body, causing poisoning symptoms by combining with other substances, and nitrate nitrogen which is a source of producing carcinogenic substances, The current situation is that no technology has been developed to actively remove it from roughage.
本発明は、上記の事情に鑑みなされたもので、粗飼料
を摂取して体内に入ってから有毒物質となる硝酸態窒素
を簡易に除去できる方法を提供することを目的とする。The present invention has been made in view of the above circumstances, and an object thereof is to provide a method for easily removing nitrate nitrogen, which is a toxic substance, after ingesting a roughage and entering the body.
上記の目的を達成するために本発明は、硝酸態窒素を
含有している粗飼料に、酵母の中で同化型硝酸塩還元酵
素を有する菌株であるピキア（Pichia anomala）属菌
種キラー株を用い、これに乳酸菌のラクトバチルス プ
ランタルム（Lactobacillus plantarum）KTC4−３株を
併用接種し、この酵素を利用して硝酸態窒素を還元後、
さらに菌体成分に変換し増殖させて、粗飼料から硝酸態
窒素を除去するようにしたことを特徴とするものであ
る。In order to achieve the above object, the present invention, in a roughage containing nitrate nitrogen, Pichia (Pichia anomala) genus species killer strain, which is a strain having assimilating nitrate reductase in yeast, Lactobacillus plantarum (Lactobacillus plantarum) KTC4-3 strain was inoculated together with this, and after using this enzyme to reduce nitrate nitrogen,
Further, it is characterized in that nitrate nitrogen is removed from the roughage by converting it into a bacterial cell component and growing it.
上記の方法によって、硝酸窒素は、酵母および乳酸菌
によって還元され、さらに菌体成分に変換されて除去さ
れ、中毒症状を起こしたり、発ガン性物質を生成したり
しない。By the above-mentioned method, nitrogen nitrate is reduced by yeast and lactic acid bacteria, further converted into bacterial cell components and removed, and neither poisoning symptoms nor carcinogenic substances are produced.
以下、図面および別表を参照して、本発明を粗飼料と
してのスイートソルガムを材料として用いた実施例につ
いて説明する。Hereinafter, with reference to the drawings and the attached table, examples of using the present invention as a raw material for sweet sorghum will be described.
（１）実施手法 ［使用菌株］ 酵母は、保存・収集株、分離株計20株強の中からサイ
レージに常在的なピキア（Pichia）属菌種で、硝酸塩同
化能、蔗糖同化能が比較的強いキラー株を使用した。乳
酸菌は、ラクトバチルス プランタルム（Lactobacillu
s plantarum）KTC4−３株を用いた。酵母は、酵母エキ
ス0.1％加用ツァペック ドックス（Czapec Dox）培地
にて３日間振盪培養後、乳酸菌は同じくMRS培地にて２
日間培養後、表１に示す接種菌数（inoculum size）で
接種した。(1) Implementation method [Strains used] Yeast is a storage and collection strain and a total of more than 20 isolates, and is a Pichia species that is resident in silage. A strong killer strain was used. Lactobacillus is Lactobacillus plantarum.
s plantarum) KTC4-3 strain was used. The yeast was shake-cultured in yeast extract 0.1% Czapec Dox medium for 3 days with shaking, and the lactic acid bacteria were also cultured in MRS medium in 2%.
After culturing for one day, the cells were inoculated with the inoculum size shown in Table 1.
［材 料］ 実験1,2（予備試験）では、圃場にて栽培したスイー
トソルガムを材料とし、微切断後、３本ロール型搾汁機
にて茎部のみの残渣を調製後、一処理区当り新鮮物10k
g、同じく実験３（本試験）では、別に調製した残渣を1
40kg/区供試した。その際、実験１では、硝酸カリウム
をNO3をして0.4％／新鮮物、同じく実験2,3では0.2％補
足した。[Materials] In Experiments 1 and 2 (preliminary test), sweet sorghum cultivated in the field was used as a material, and after finely cutting, a residue of only the stem was prepared with a three-roll type squeezing machine. 10k fresh food
g, also in Experiment 3 (this test), 1
40 kg / district tested. At that time, in Experiment 1, potassium nitrate was added to NO 3 to supplement 0.4% / fresh matter, and in Experiments 2 and 3, 0.2% was supplemented.
［処理区］ ３回の実験とも表２に示す４処理区により実施した。[Treatment Section] The three treatments were carried out by the four treatment sections shown in Table 2.
［前処理工程］ 酵母および乳酸菌を接種後の残渣は、予備試験では厚
さ3.5cm、同じく本試験では15cmにて室内に２日間堆積
した。この間３〜５回繰り返し（第１図参照）を行った
後、材料はサイロに詰めた。[Pretreatment step] The residue after inoculation with yeast and lactic acid bacteria was deposited in the room for 2 days at a thickness of 3.5 cm in the preliminary test and 15 cm in the same test. During this time, after repeating 3 to 5 times (see FIG. 1), the material was packed in silos.
［分析・測定法等］ 水分は凍結乾燥法、硝酸態等はイオンクロマトグラフ
法およびブレムナー（Bremner）法の併用により、pH等
の物理・化学的測定は慣行の方法、微生物数の計算等は
希釈平板により行った。[Analysis / Measurement method] Freeze-drying method is used for water, ion chromatography method and Bremner method are used for nitrate state, etc. Diluted plates were used.
（２）実施結果 ．予備試験では、接種２日後、酵母接種区（3,4区）
の品温は、酵母無接種区（1,2区）に比較し約３℃高温
にあった。また、本試験においては、接種約16時間後よ
り品温の上昇が認められた。特に酵母と乳酸菌を併用し
た４区で発熱が顕著であった（第１図参照）。(2) Implementation results. In the preliminary test, 2 days after the inoculation, the yeast inoculation area (3, 4 areas)
The product temperature was about 3 ° C higher than that in the non-inoculation area (1, 2 areas). In addition, in this test, an increase in the product temperature was observed about 16 hours after the inoculation. In particular, fever was remarkable in the 4 groups where yeast and lactic acid bacteria were used together (see FIG. 1).
.3回の実験とも、乳酸菌接種区（2,4区）において乳
酸菌数の顕著な増加は認められなかった。一方、酵母は
著しい増殖を示し、3,4区で8.88〜9.67cfu/g（常用対数
表示、集落形成単位）に達した（表２参照）。.3 No significant increase in the number of lactic acid bacteria was observed in the lactic acid bacteria inoculated area (2, 4 areas). On the other hand, yeast showed remarkable growth, reaching 8.88 to 9.67 cfu / g (common logarithmic display, colony forming unit) in the 3rd and 4th plots (see Table 2).
．いずれの実験においても、酵母接種区では、酵母無
接種区に比較し、硝酸態窒素（NO3）の消失が顕著であ
り、特に予備試験では66.5〜76.2％と、酵母無接種区の
約７倍も高い消失を示した（表２参照）。. In all of the experiments, the loss of nitrate nitrogen (NO 3 ) was more remarkable in the yeast inoculation group than in the yeast non-inoculation group. Especially, 66.5 to 76.2% in the preliminary test, about 7 It showed twice as high disappearance (see Table 2).
.2日間を通じ、いずれの材料においても亜硝酸（N
O2）は不検出（第２図参照）であり、アンモニア態窒素
も不検出〜極微量であった。また、酵母菌はこの間に顕
著なcfuの増加をみていることにより、酵母接種区で消
失したNO3はそのまま菌体成分へ変換・除去されたとい
える。.Nitrite (N
O 2 ) was not detected (see FIG. 2), and ammonia nitrogen was also not detected to a very small amount. In addition, since the yeast has seen a remarkable increase in cfu during this period, it can be said that NO 3 that disappeared in the yeast inoculation zone was converted and removed as it was into the bacterial cell component.
なお、サイロは未開封であるが、PVCサイロへ詰めて
調製したサイレージ（予備試験）では、外観上いずれの
サイロでも、不良発酵の発生は感知されていない。Although the silo is unopened, the silage prepared by filling it into a PVC silo (preliminary test) did not detect the occurrence of defective fermentation in any silo in appearance.
この試験結果から、本発明の硝酸態窒素の除去方法
は、牧草や飼料用長大作物（粗飼料）およびそれらを発
酵して調製するサイレージや同様に硝酸態窒素を含 有し、漬物に加工するニンジン、ダイコン等の園芸野菜
にも同様に適用できるものである。From this test result, the method for removing nitrate nitrogen of the present invention shows that long crops for grass and feed (rough forage) and silage prepared by fermenting them and similarly containing nitrate nitrogen. It is also applicable to garden vegetables such as carrots and radish that are processed into pickles.
以上説明したように本発明の粗飼料における硝酸態窒
素の除去方法によれば、硝酸態窒素を含有する粗飼料に
対し、硝酸塩同化能を有する酵母および乳酸菌を接種す
るだけで硝酸態窒素を菌態成分に変換し、消去，除去で
きるので、その作業は簡単であり、従来、体内に入って
中毒症状を起こしたり、発ガン性物質を生成していた危
険を、未然に防止することができる。As described above, according to the method for removing nitrate nitrogen in the roughage of the present invention, the roughage containing nitrate nitrogen, by inoculating the yeast and lactic acid bacteria having nitrate assimilation ability, the nitrate nitrogen is a fungal component. Since it can be converted into, erased, and removed, the work is simple, and it is possible to prevent the danger of entering into the body and causing poisoning symptoms or producing carcinogens.
第１図は本発明の一実施例を示し、酵母、乳酸菌を接種
後の材料品温の推移を示すグラフ、第２図はスイートソ
ルガム搾汁残渣の水抽出液のイオンクロマトグラムであ
る。FIG. 1 shows an embodiment of the present invention, and is a graph showing changes in material temperature after inoculation with yeast and lactic acid bacteria, and FIG. 2 is an ion chromatogram of a water extract of sweet sorghum juice residue.
Claims (1)
の中で同化型硝酸塩還元酵素を有する菌株であるピキア
（Pichia anomala）属菌種キラー株を用い、これに乳
酸菌のラクトバチルス プランタルム（Lactobacillus
plantarum）KTC4−３株を併用接種し、この酵素を利
用して硝酸態窒素を還元後、さらに菌体成分に変換し増
殖させて、粗飼料から硝酸態窒素を除去するようにした
ことを特徴とする粗飼料における硝酸態窒素の除去方
法。1. A killer strain of Lactobacillus plantarum which is a lactic acid bacterium is used as a roughage containing nitrate nitrogen, in which a killer strain of the genus Pichia anomala, which is a strain having assimilating nitrate reductase in yeast, is used. (Lactobacillus
plantarum) KTC4-3 strain was inoculated together, nitrate nitrogen was reduced using this enzyme, and then converted to bacterial components and grown to remove nitrate nitrogen from the roughage. Method for Removing Nitrate-Nitrogen from Roughage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2338615A JP2674674B2 (en) | 1990-11-30 | 1990-11-30 | Removal method of nitrate nitrogen in roughage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2338615A JP2674674B2 (en) | 1990-11-30 | 1990-11-30 | Removal method of nitrate nitrogen in roughage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04207160A JPH04207160A (en) | 1992-07-29 |
| JP2674674B2 true JP2674674B2 (en) | 1997-11-12 |
Family
ID=18319848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2338615A Expired - Lifetime JP2674674B2 (en) | 1990-11-30 | 1990-11-30 | Removal method of nitrate nitrogen in roughage |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2674674B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102316396B1 (en) * | 2020-10-28 | 2021-10-26 | 한국식품연구원 | Lactobacillus plantarum WiKim0112 having nitrates-scavenging ability and composition comprising the same |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0805205A1 (en) * | 1996-05-02 | 1997-11-05 | Societe Des Produits Nestle S.A. | Nitrate reduction system of Staphylococcus carnosus |
| JP5470637B2 (en) * | 2009-07-01 | 2014-04-16 | 独立行政法人農業・食品産業技術総合研究機構 | Microorganisms that reduce nitrate nitrogen and nitrite nitrogen in fermented feed |
| JP6769615B2 (en) * | 2017-04-20 | 2020-10-14 | 有限会社オフイスヨコオ | Nitrate nitrogen removal method and nitrate nitrogen remover |
| CN119842504A (en) * | 2024-12-26 | 2025-04-18 | 中国农业大学 | Microorganism, microbial agent, food, method for producing food, and method for inhibiting growth of pathogenic bacteria in vitro |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52143272A (en) * | 1976-05-24 | 1977-11-29 | Morinaga & Co | Decomposition of xanthine derivative by imcroorganism |
| GB8319540D0 (en) * | 1983-07-20 | 1983-08-24 | Bovril Ltd | Amine removal |
-
1990
- 1990-11-30 JP JP2338615A patent/JP2674674B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102316396B1 (en) * | 2020-10-28 | 2021-10-26 | 한국식품연구원 | Lactobacillus plantarum WiKim0112 having nitrates-scavenging ability and composition comprising the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04207160A (en) | 1992-07-29 |
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