JP2678617B2 - Large garlic extract - Google Patents

Description

Detailed Description of the Invention The present invention was developed to overcome the drawbacks of the conventional methods for producing a comprehensive garlic active ingredient, and the ginger extract produced according to the present invention is used as a medicine. be able to.

Conventional technology In the conventional extraction method aimed at odorless substances of large garlic, odorization was observed due to imperfect enzyme deactivating means, and in the case of obtaining the essential oil of large garlic, the degree of enzyme activity It was observed that the yield was reduced due to the incompleteness of the oil, the composition was biased, and the drug efficacy of the substance after the essential oil was obtained was decreased.

Problems to be Solved by the Invention In methods for obtaining odorless substances of large garlic, a heating method is generally used as an enzyme deactivating means. Destruction of the active ingredient was observed, and a large amount of essential oils and other impurities remained due to omission or inadequacy of the purification process.As a result, these substances show markedly better pharmacological effects than the substances of the present invention. It was inferior.

Means for Solving the Problems As a characteristic of the present invention, as a result of various studies conducted from the above viewpoints, the product was heated in water and uniformly deactivated to obtain a squeezed juice. Water-soluble proteins are removed by adding iron hydroxide free of iron ions to, neutralized and decolorized, and then extracted with methanol. The concentration of the liquid is always 60
The treatment was carried out under reduced pressure at ℃ or below to avoid destruction of various active ingredients including scordinine homologues. Next, for removing impurities, activated carbon was used for adsorption, an aqueous solution was adsorbed, and elution was carried out with methanol, and then fractane was removed with barium hydroxide as much as possible.

LD of Acute Toxicity Test for Ginseng Extract Component According to the Present Invention
₅₀ values were 14.5 g / kg for males and 17.4 g / for females by intraperitoneal injection in mice.
kg, subcutaneous injection was 19.6 g / kg for males and 21.7 g / kg for females, and oral administration was 50.0 g / kg or more for both males and females. LD _{50 in} rats
Values are male 11.8 g / kg female 14.3 g / kg by intraperitoneal injection, male by subcutaneous injection
26.1 g / kg females were 22.8 g / kg, and oral administration was 40.0 g / kg or more in both males and females.

The production method of this substance and the drug using this substance will be described below according to Examples.

Example 1 1 kg of garlic is taken, heated in a boiling water bath for about 15 minutes and then squeezed. About 100 ml of squeezed juice was added with 1.5 g of iron hydroxide, left at room temperature for 2 days with occasional stirring to remove water-soluble proteins, neutralized with precipitated calcium carbonate, and added with 10 g of activated carbon, and then filtered. Was filtered under pressure to obtain an almost colorless and transparent neutral liquid. This is concentrated under reduced pressure at 60 ° C or below to about 30 g, the concentrate is dissolved in an appropriate amount of water, 30 g of activated carbon is added to make it adsorb, and then washed with water 1, and the adsorbed product is made up to about 300 ml of methanol at 60 ° C. Leach for 5 hours. This leachate at 60 ° C
Concentrate below to 11 g, dissolve the concentrate in 50 ml of water and filter with stirring to add barium hydroxide solution until no more fructans precipitate. After removing excess barium hydroxide with carbon dioxide and dilute sulfuric acid, the filtrate is concentrated under reduced pressure at 60 ° C or lower to obtain a concentrated liquid. The concentrated liquid was dried under reduced pressure at 60 ° C. or lower, pulverized, and sieved with 40 mesh to obtain 10 g of a garlic extract component.

Example 2 50 g (all by weight below) of the ginger extract obtained in Example 1 and 950 g of an excipient such as potato starch and calcium hydrogen phosphate were uniformly mixed to obtain 100 g of a powder.

Example 3 To 36 kg of the garlic extract component obtained in Example 1 was added 35.5 kg of excipients such as potato starch, calcium hydrogen phosphate, lactose, etc., and the mixture was uniformly mixed, compressed into tablets and sugar-coated to give tablets 1680.
I got 0 tablets.

Example 4 500 mg of the garlic extract component obtained in Example 1 was dissolved in 60 ml of water, and a so-called drink was prepared by a conventional method.

Example 5 200 mg of the ginger extract obtained in Example 1 was mixed with an excipient such as potato starch to give a total amount of 500 mg, and a so-called capsule was manufactured by a conventional method.

In addition to Examples 2 to 5, the ginger extract obtained in the present invention can be used to produce injections and various pharmaceuticals in other dosage forms.

Effects of the Invention The pharmaceuticals obtained in the above examples are used for medical purposes as fatigue recovery, weak constitution, appetite enhancement, neuralgia, rheumatism, arthritis, gestational addiction, menopausal disease, lack of milk, increased physical fitness of tuberculosis disease, and sweating. , There are various indications for metabolic disorders and allergic diseases, and over-the-counter drugs include indications for weak constitution, physical fatigue, post-disease illness, gastrointestinal weakness, loss of appetite, and nutritional tonic during development. The above-mentioned various indications have been proved as a result of pharmacological experiments and clinical experiments conducted in medical institutions and the like nationwide in cooperation with the present inventor.

It should be noted that, as a part of the manufacturing method of this product, substances having the same active ingredients as targets are being used for health foods, functional foods and the like.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display area A61K 35/78 ACM A61K 35/78 ACM ADD ADD

Claims (3)

(57) [Claims] 1. A squeezed juice obtained by heating garlic in boiling water for 15 to 30 minutes and then squeezing it to 1 to 2% thereof.
Iron hydroxide is added to remove the precipitated water-soluble protein, and calcium carbonate is added to the filtrate to neutralize and remove organic acids,
Activated carbon is added to decolorize, then filtered, and concentrated under reduced pressure at 60 ° C or lower. Methanol was added to this, and the mixture was shake-extracted, and the filtrate was concentrated under reduced pressure at 60 ° C. or lower to give a concentrate as an aqueous solution.
Activated carbon corresponding to 2 to 4% of the raw material is added for adsorption and then washed with water, and the adsorbed substance is leached with methanol at 60 ° C. corresponding to 20 to 40% of the raw material. The leachate is concentrated below 60 ° C., the concentrate is dissolved in water and the barium hydroxide solution is added with stirring until the fructans no longer precipitate, and then filtered.
After removing excess barium hydroxide with carbon dioxide and dilute sulfuric acid, the filtrate is concentrated under reduced pressure at 60 ° C or lower. This concentrate
A garlic extract component characterized by being dried under reduced pressure at 60 ° C or lower. 2. A pharmaceutical product containing, as a main component, the garlic extract component according to claim 1. 3. A pharmaceutical product containing the garlic extract component according to claim 1.