JP2740658B2 - Intestinal harmful bacteria growth inhibitor - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to an agent for inhibiting the growth of harmful intestinal bacteria, which contains flesh of evening glow as an active ingredient.

Prior art Evening glory is an annual vine plant of the bollaceae, and its fruit is oval with a diameter of about 70 cm. The dried and dried pulp of the fruit is like a string.

Hitherto, it has not been known that the flesh of the evening face suppresses the growth of intestinal harmful bacteria.

Problems to be Solved by the Invention Since the known bifid factor is used also for other intestinal bacteria in a living body, the growth effect of bifidobacterium is not sufficient, and the production method thereof is complicated. Disadvantages such as high cost. Therefore, a bifidobacterium having sufficient bifidobacterium growth promoting effect and intestinal harmful bacterial growth inhibitory effect, as well as being inexpensive and easily producible, is extremely useful in industry.

Means for Solving the Problems Already, some of the present inventors have conducted intensive studies on the bifidobacterium, and as a result, the evening meal obtained by processing the evening flesh selectively and remarkably promoted the growth of Bifidobacterium bacteria. (Japanese Patent Application No. 62-123761). The present inventors have found that the evening pulp has an effect of suppressing the growth of intestinal harmful bacteria, and completed the present invention.

The intestinal harmful bacteria growth inhibitor of the present invention powders evening flesh,
It is processed into a desired shape such as granules and tablets, and is sterilized or not.

The sterilized intestinal harmful bacteria growth inhibitor of the present invention has a vapor pressure of 0.2 to 0.4 kg / cm ² , a sterilization time of 5 to 20 minutes by steam sterilization or an alcohol addition amount of 15 to 150 g / kg (evening dried product), It can be manufactured by alcohol sterilization for 1 to 30 days of sterilization. This sterilized intestinal harmful bacteria growth inhibitor has a general viable cell count of 5 × 10 ³ cells / g or less, is negative for Escherichia coli and Salmonella, and has a water content of 8% or less, and is extremely safe in food hygiene.

Additives may be added to the intestinal harmful bacteria growth inhibitor of the present invention.Examples of additives include sweet corn, carrot powder, corn fiber, apple fiber, pumpkin powder, fiber components such as alginic acid, lactose,
Excipients such as starch, sucrose, maltose, sorbitol,
Supplementary ingredients such as mannitol, milk powder, meat extract, vitamins, etc., gum arabic powder, polyvinylpyrrolidone, binders such as hydroxypropylcellulose, sodium carboxymethylcellulose, magnesium stearate, calcium stearate, Lubri wax, talc And the like, and may be appropriately selected and used depending on the shape and / or preference of the growth inhibitor.

Among the intestinal harmful bacteria growth inhibitor of the present invention, powdery or granular ones may be added as soup ingredients, sprinkles, or to confectionery, noodles, tofu, or other foods. In addition,
It can also be used as a feed additive for livestock such as cattle and pigs, and as a feed additive for pets such as dogs and cats.

Action The intestinal harmful bacteria growth inhibitory action of the evening meal pulp powder of the present invention will be described.

Test 1 As a sample, an evening pulp flour powder manufactured by the same operation as in Example 1 and a sterilized night pulp powder manufactured by the same operation as in Example 5 were used.

Each sample was prepared using Tomarelli modified medium (Journal of Biochemical Chemistry, 181).
Vol. 879, 1949), and Bifidobacterium and other enteric bacteria were cultured at 37 ° C for 48 hours. After the culture, the culture solution was applied to a BL agar medium, cultured at 37 ° C. for 48 hours, and the number of bacteria was measured. Table 1 shows the results.

As is evident from Table 1, the evening meal pulp powder and the pasteurized evening meal pulp powder show a remarkable growth promoting effect against bifidobacteria, while enterococci, Staphylococcus aureus, E. coli,
It is not used for intestinal harmful bacteria such as C. perfringens,
It shows a growth inhibitory effect on these bacteria.

Example 1 The outer skin of the evening face was peeled thinly, and the pulp was sliced into a thin strip,
After drying in the sun for 1 day, the mixture was dried at 70 ° C. for 5 hours to obtain zonal evening pulp with a water content of about 3%. 30 kg of this dried product is pulverized with a pulverizer (manufactured by Hosokawa Iron Works) and 240 kg of evening flesh powder
I got

Example 2 After mixing 950 g of the powder obtained in Example 1 and 40 g of hydroxypropylcellulose in a mixing stirrer for 10 minutes, purified water was added and kneaded, followed by hot-air drying. After allowing it to cool, it is sized with an oscillator (manufactured by Kikusui Seisakusho) and the evening flesh granules 96
0 g was obtained.

Example 3 After mixing 495 g of the granules obtained in Example 2 and 5 g of magnesium stearate in a mixing stirrer for 10 minutes, the mixture was tableted to obtain 4,400 tablets of 110 mg of evening meal flesh tablets.

Example 4 30 kg of zonal evening pulp obtained in the same manner as in Example 1 was added to 0.2 kg /
After sterilization for 20 minutes under a steam pressure of cm ² , the water content is about 3%
It was dried with hot air at 60 ° C until the temperature became. Then, the same operation as in Example 1 was carried out to obtain 25 kg of sterilized evening pulp powder.

This powder had a general viable cell count of 2.0 × 10 ² cells / g and was negative for E. coli and Salmonella.

Example 5 Ethanol (2.7 kg) was sprayed on 30 kg of zonal evening pulp obtained in the same manner as in Example 1 (90 g / kg zonal evening pulp), sealed, and allowed to stand at room temperature for 5 days. As a result, 25.7 kg of sterilized evening pulp powder was obtained.

This powder had a general viable count of 37 cells / g and was negative for Escherichia coli and Salmonella.

Effect of the Invention The intestinal harmful bacteria growth inhibitor of the present invention selectively and remarkably promotes the growth of Bifidobacterium bacteria and suppresses the growth of harmful intestinal bacteria, and thus is useful for maintaining health and improving immunity. It is also expected as a drug such as an activator or an anticancer drug.

Claims (1)

(57) [Claims] 1. An agent for inhibiting the growth of harmful enteric bacteria selected from enterococci, Staphylococcus aureus, Escherichia coli and C. perfringens, which contains evening pulp as an active ingredient.