JP2764497B2 - Culture method of mycorrhizal mushrooms - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention is used for producing a medium for inoculum or cultivation, such as matsutake mushroom, shimejime, matsude, etc., of which mycelial mushrooms are extremely slow in mycelial growth. To a method for culturing mycorrhizal mushrooms as described above.

[0002]

2. Description of the Related Art Conventionally, methods for inoculating and cultivating mushrooms of this kind into a culture medium include the following two methods for inoculum or cultivation. The first method is to use a bottle or a bag of heat-resistant film, fill the medium with the medium, then make one or more holes from the surface to the bottom of the medium, sterilize and cool it. , Inoculate the inoculum from the bottle mouth,
This is a method in which bacteria are attached to the upper surface of the medium and the inner surface of the hole, and the mycelium is extended into the medium from there. The second method is a large-scale production method generally called a batch method. In this method, a large amount of culture medium is put in a large-capacity container equipped with a stirrer, sterilized, cooled, inoculated with a seed bacterium, stirred, and then divided into small containers and stored in the container. The mycelium is extended over the entire medium.

[0003]

In the first method, since the mechanization of the filling of the medium and the inoculation of the inoculum can be easily performed and the capital investment is small, at present, this method is almost used for the inoculation of the inoculum. Have been. However, the use of this method for cultivation of mushrooms such as matsutake mushrooms is disadvantageous because the hypha elongation of the inoculum inoculated by making a hole in the medium is extremely slow.

[0004] The second method is advantageous in that hyphal elongation occurs from the whole medium. However, in addition to the increased capital invested in the equipment, the large volume of inoculum to be inoculated makes it difficult to check for a small amount of various bacteria mixed in the large-capacity inoculum. However, there is a risk that the culture must be re-cultured. That is, in the case of strains such as Shiitake fungi and Hitake mushrooms, in which the growth of hyphae is fast, the growth of mixed bacteria can be suppressed, so that this method can be used relatively easily. Is more dangerous because it can be used for the growth speed of various bacteria.

[0005] In view of the above, in mycorrhizal mushrooms with slow hyphal elongation, it is possible to cultivate a large-capacity solid culture for seeds and fungal beds as quickly and stably as possible. I have been asked. In particular, it has been desired to solve this problem in fungi, such as matsutake fungi and fungi, which naturally elongate mycelia but whose cultivation cannot be performed artificially.

[0006]

SUMMARY OF THE INVENTION In order to solve the above-mentioned problems, the present invention provides an appropriate amount of culture medium and mycorrhizal mushroom in a film culture bag having appropriate heat resistance and flexibility. After containing the bacteria, the mouth of the bag is closed and rubbed, force is applied from the outside, and the contents in the bag are appropriately flowed and inverted, and the mycorrhizal characteristic is characterized by sufficiently mixing the inoculum and the medium material. It is intended to provide a method for culturing mushrooms.

[0007]

In the medium inoculated with the inoculum according to the present invention, the inoculum is mixed in an appropriate volume with the entire inoculum. As soon as possible, it grows quickly. In addition, there is a relatively low probability of being infested by various germs, and there is less damage as compared with the batch method.

[0008]

EXAMPLE An aqueous solution obtained by dissolving 2% glucose, 0.2% yeast extract (manufactured by Daigo Pharmaceutical), 0.1% magnesium sulfate, 0.1% dipotassium phosphate, 5ppm thiamine hydrochloride and 5ppm folic acid in water. And 800 cc of the above aqueous solution was added to 110 g of peat moss, and adjusted to a pH of 5.2 with 0.1 N NaOH to prepare a medium material. Seed is Matsutake Tr
icholoma matutake IFO. The 6933 strain was used. As the container, a polypropylene container was used, and the medium was filled so as to have a diameter of 11 _cm and a height of 10.5 _cm .

Then, as shown in FIGS. 2 and 3, a medium having a hole 1 _cm in diameter is formed in the center of the medium 2 accommodated in the bag 1.
Four holes with a diameter of 1 _cm were formed. In the example of the present invention, as shown in FIG. 1, a mushroom culture bag formed of a polypropylene film and having a diameter of 11 cm and a capacity of about 1500 cm ³ was formed into a cylindrical shape. The culture bag 1 used in the present invention is described in, for example, JP-A-3-61477.
No. reference. The containers of the four examples described above were filled with the same volume of the culture medium 2 and sterilized and cooled, and then 20 cm ³ of the solid culture inoculum 3 was added to each.

In the case of the embodiment of the present invention, immediately after the inoculum is added, the mouth of the culture bag 1 is closed and rubbed to apply a force from the outside, so that the contents in the bag can be flowed and inverted appropriately to sufficiently. The mixture was compared with the mixture obtained by adding the inoculum 3 and culturing for one month and then performing the above-described mixing operation.
The culture was performed at 23 ° C.

[0011]

[Table 1] In Table 1, 10 containers and bags having the same capacity were used for each example, and the number of days at which the mycelium was completely extended to the entire medium was expressed as an average value. As a result, when the medium and the inoculum were mixed in a soft bag, the number of days over which the mycelium extended to the entire medium was about 1 day, compared to the case where the medium was inoculated with a hole in the container.
５〜 to １ ／.

[0012]

As described above, in the culture of mushrooms having a slow hypha elongation, a flexible bag is used, and the culture medium and the inoculum are appropriately flowed, inverted, and sufficiently mixed in a bag with a closed mouth. According to the present invention, a solid culture mycelium can be obtained remarkably faster than a conventional method. Further, this method is extremely useful for inoculum production and mushroom cultivation also in that a large amount of young mycelium having strong activity is obtained.

[Brief description of the drawings]

FIG. 1 is an explanatory diagram of the present inoculation method.

FIG. 2 is an explanatory view showing a conventional inoculation method.

FIG. 3 is an explanatory view showing a conventional inoculation method.

[Explanation of symbols]

 1 culture bag 2 medium material 3 inoculum

Continuation of front page (58) Field surveyed (Int.Cl. ⁶ , DB name) A01G 1/04

Claims (1)

(57) [Claims] 1. An appropriate amount of a culture medium 2 and mycorrhizal mushroom inoculum 3 are accommodated in a film culture bag 1 having appropriate heat resistance and flexibility, and then the bag is closed and rubbed. A method for cultivating mycorrhizal mushrooms, which comprises applying a force from the outside, appropriately flowing and inverting the contents in the bag and sufficiently mixing the inoculum 3 and the medium 2.