JP2782335B2 - Propolis aqueous dispersion - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a stable aqueous dispersion of propolis, a crab-like substance collected from the entrance of a beehive, and a method for producing the same.

[0002]

2. Description of the Related Art Propolis is a tar-like substance existing at the entrance and exit of a beehive such as a bee and has an antibacterial action. In other words, for bees, the beehive is a sanctuary, inhabiting about 20,000 queen bees, many larvae and worker bees, and is the basis of their life and reproduction. If a worker bee becomes contaminated with pathogens, it means that one group is exterminated. Therefore, an antibacterial spider-like substance exists at the entrance of the beehive, and the safety of the beehive is ensured by bringing the whole body of the bee in and out into contact with the spear-like substance. The tarnish-like natural antibacterial substance is propolis, which is traded as a black tar-like mass.

[0003] This propolis is a natural antibacterial substance that does not cause resistant bacteria to appear on the human body.
It has a remarkable effect when used for prevention and treatment of infectious diseases such as Trichophyton and viruses. Furthermore, it is effective for promoting blood circulation, preventing and treating cancer, enhancing gastrointestinal function, burns and atopy, has a favorable pharmacological action, and is effective in a small amount. However, this material is water-insoluble and propolis 20-
Commercially available as a solution of 35% and about 65% of ethanol,
For drinking, a few drops of a concentrated propolis alcohol solution are dropped into water.

[0004]

However, the alcohol solution of propolis dropped into water immediately precipitates,
Part of the active ingredient forms a yellow film and covers the surface of water,
It adheres to the vessel wall of the container and further to the teeth, discoloring the teeth. In addition, yellow stains adhered to containers and teeth cannot be removed by a normal cleaning method, but must be cleaned with an alcohol solution. Such characteristics mean that a part of the active ingredient is not taken, and in such a state, even if the whole amount is taken, there is a possibility that the active ingredient is not sufficiently absorbed in the gastrointestinal tract.

[0005] Further, since it is a food and drink, only a solvent other than water can be used except ethanol, and even if ethanol is used, it is a drug having side effects for children and people who do not like alcohol. Propolis tablets are also commercially available, but have a drawback in that the propolis itself is an aggregate of a large number of water-insoluble molecules, so that its absorption in the gastrointestinal tract is poor and its original effect cannot be sufficiently exhibited. Under these circumstances, there has been a demand for a stable aqueous dispersion that does not use alcohol and does not separate for a long period of time in order to make it easier to ingest propolis having excellent efficacy.

[0006]

SUMMARY OF THE INVENTION An object of the present invention is to solve the above-mentioned problems, and an object of the present invention is to provide an aqueous suspension of propolis containing cyclodextrin and containing no substance having a surfactant effect. Characterized in that the propolis is dispersed in fine particles having an average particle diameter of 5 μm or less,
Further, a concentrated solution of cyclodextrin is added to an alcoholic solution of propolis, and the mixture is ultrasonically dispersed to be dispersed in fine particles of propolis. After inclusion in the cyclodextrin, heating is performed to sterilize and volatilize the alcohol. It is characterized by the following.

[0007] The present inventor has attempted to enclose propolis dissolved in ethanol with cyclodextrin. That is, cyclodextrin was added to an ethanol solution of propolis, water was added, and the mixture was shaken well. As a result, a uniform aqueous dispersion of propolis was obtained.
After 20 minutes, propolis had separated. This tendency was not changed even when the amount of cyclodextrin was increased and the amount of propolis was decreased, and it was confirmed that a long-term stable aqueous dispersion of propolis could not be obtained even with cyclodextrin. From this fact, it was assumed that various component molecules of propolis were associated with each other and were in a state too large to be included by cyclodextrin.

Therefore, an ultrasonic treatment having an emulsifying and dispersing action was performed. However, when water was added to the ethanol solution after the ultrasonic treatment, the separated propolis soon precipitated in a film form on the liquid surface, and a long-term stable aqueous dispersion of propolis was not obtained even by the ultrasonic treatment. .

The inventor of the present invention has found that when ultrasonic treatment is performed on an ethanol solution of propolis in the presence of cyclodextrin, a stable aqueous dispersion of propolis can be obtained by the synergistic effect of the coexistence of cyclodextrin and ultrasonic treatment. To complete the present invention. The aqueous dispersion of the present invention was then added with other components, sterilized by heating, bottled, and stored at room temperature. However, even after one year, the aqueous dispersion still maintains a uniform dispersion state. Moreover, even if this dispersion is mixed with water, the homogeneity of the dispersion is not lost, an aqueous dispersion having a light sweetness and a slight aroma is obtained, and drinking becomes extremely easy,
There is no dirt on the container and no dirt on the teeth, and since the propolis itself is dispersed as fine particles, it absorbs well and can exhibit more excellent effects.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION Propolis in the present invention is obtained by separating natural antibacterial components attached to the entrance of a honeycomb. This component itself is considered a mixture of various substances, but is a black tar-like mass. As a raw material of the present invention, a black-brown transparent liquid obtained by extracting the above components with ethanol, that is, a propolis extract is used as a raw material. This liquid is generally commercially available and available as a propolis solution. That is, propolis 20-35
W / V%, 65 W / V% ethanol solution. When used as a raw material of the present invention, the concentration of propolis is not particularly limited as long as propolis is transparently dissolved in ethanol in advance. Ethanol solution is 60-70W / V
% Is common. Hereinafter, the term propolis refers to components other than alcohol in this alcohol extract.

Cyclodextrin is a crown-shaped dextrin in which 6 to 12 glucoses are linked by α-1,4-glucoside and is a cyclic oligosaccharide having excellent crystallinity. Cyclodextrin is known to have the property of including various molecules in a crown-shaped space. Cyclodextrin is used as a 50-80% concentrated solution. The amount of cyclodextrin is 1 to 50 g per 1 g of propolis,
Preferably it is 5 to 40 g, more preferably 10 to 20 g. If the amount of cyclodextrin is less than 1 g per 1 g of propolis, the propolis is not reliably included and there is a problem in the stability of the water suspension. If the amount exceeds 50 g, the cyclodextrin is used wastefully and products that are too sweet can get.

As the ultrasonic waves, various types of ultrasonic oscillators having commercially available processing tanks can be used. A concentrated solution of propolis extract and cyclodextrin is placed in a treatment tank and subjected to ultrasonic treatment. The processing time varies depending on the output of the ultrasonic oscillator, the amount and concentration of the propolis extract, and the processing time. By performing the ultrasonic treatment in the presence of cyclodextrin, the finely divided propolis particles are included in the cyclodextrin in situ, and a stable aqueous dispersion is obtained.

When performing ultrasonic treatment, 30 to 65
When heated to a temperature of 40 ° C, preferably 40 to 60 ° C, the propolis is efficiently dispersed. In addition, during sonication, a vortex is generated in the sonication tank, and at first glance it seems to be sufficiently stirred. However, in order to further improve the frequency of collision between the propolis particles and the cyclodextrin molecules, it rotates at low speed It is preferable to provide a stirring means. That is, the mixing of the stirring in different directions into the vortex causes a complicated turbulence, and the dispersion efficiency is improved. When sufficient emulsification is performed, the liquid in the processing tank becomes sticky. When the ultrasonic treatment is stopped before this state is obtained, the obtained aqueous dispersion has poor stability and separates after 10 minutes to several hours.

The propolis dispersion obtained by the above method contains 10 to 200 g, preferably 20 to 60 g, of honey and 1 to 10 g of propolis per 1 g of propolis.
300 g, preferably 30-150 g, of a concentrated aqueous solution of the oligosaccharide are added. Further, other additives such as royal jelly can be added as needed. Propolis in the obtained aqueous dispersion is fine particles in a state of being included in cyclodextrin, and has an average particle size of 5 μm or less, preferably 3.5 μm or less, more preferably 2.5 μm or less.
μm or less. If the particle diameter of the propolis included in the cyclodextrin exceeds 5 μm, a stable aqueous dispersion cannot be obtained.

[0015]

EXAMPLES Example 1 EEP-20E from Brazil as a propolis extract
(Propolis extract, manufactured by Api Corporation) was used. The appearance is a black-brown transparent liquid. The quality of the dried residue is 20.0 W / V% or more. Total flavonoids is 0.96 W / V% or more. Ethanol content is 65.0 W / V% or less. Heavy metal is 10.0 ppm or less. It was 0 ppm or less.

A table type ultrasonic cleaner UT305 (manufactured by Sharp Corporation) was used as an ultrasonic oscillator. Maximum output 3
An ultrasonic wave of 00 W and 40 kHz is obtained. 1.5 kg of the above-mentioned propolis extract and 6.0 kg of a 75% aqueous solution of cyclodextrin were charged into this treatment tank by heating to 50 ° C., and treated at 300 W for 25 minutes while stirring with stirring blades. Since the finely divided propolis was covered with cyclodextrin and became completely loose, the ultrasonic treatment was terminated.

Honey, royal jelly and oligosaccharide were added to the propolis and cyclodextrin dispersion after the ultrasonic treatment. The ratio of each component was as follows. Raw material Weight (kg) Propolis extract (21 W / V% ethanol solution) 1.5 Cyclodextrin (75%) 6.0 Honey 16.0 Oligosaccharide (75%) 16.5 Royal jelly 1.0 Total 41.0 The propolis contained therein was 0.315 kg, the cyclodextrin was 4.5 kg, and the oligosaccharide was 12.4.
kg.

Further, this compounded liquid was mixed well at 80 ° C. and 3
Propolis dispersion obtained by sterilizing for 0 minutes and filtering
00g was bottled. In the ultrasonic treatment step and the sterilization step, the water contained in the concentrated solution of ethanol and cyclodextrin contained in the propolis extract, oligosaccharides, honey and the like evaporated. Almost no ethanol was detected in the final product. Concentrated throughout, final product 3
About 15 g of propolis was contained in 00 g. When this product was diluted with 2 to 3 times of water and drunk, moderate sweetness and slight aroma were perceived, and the propolis was uniformly and stably dispersed, and was a stable dispersion regardless of the dilution ratio. When this dispersion was examined under an electron microscope, propolis fine particles included in cyclodextrin were dispersed, and the average particle size was 2 to 2.5 μm. This product did not separate at all after one year from the production, and was a stable dispersion even when diluted many times with water.

[0019]

According to the present invention in which propolis is subjected to ultrasonic treatment in the presence of cyclodextrin, propolis which has conventionally been difficult to disperse in water and many molecules have been associated and digestion and absorptivity are unfavorable can be stabilized. We succeeded in making an aqueous dispersion.

Claims (4)

(57) [Claims] 1. An aqueous suspension of propolis containing cyclodextrin and containing no substance having a surfactant activity, wherein the propolis is dispersed in fine particles having an average particle diameter of 5 μm or less. Aqueous dispersion. 2. The aqueous dispersion of propolis according to claim 1, wherein the fine particles of propolis are fine particles obtained by subjecting an alcoholic solution of propolis to ultrasonic treatment in the presence of cyclodextrin. 3. The aqueous dispersion of propolis according to claim 1, wherein the propolis is included in cyclodextrin, and the included microparticles have an average particle size of 3.5 μm or less. 4. A concentrated solution of cyclodextrin is added to an alcohol solution of propolis, and the mixture is ultrasonically dispersed to be dispersed in fine particles of propolis. After being covered with the cyclodextrin, the mixture is sterilized by heating and alcohol is removed. A process for producing an aqueous dispersion of propolis, characterized by performing volatilization.