JP2806554B2 - Simple method for bacterial count of fibers - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial application] The present invention relates to a simple method for determining the number of bacteria in fibers, and more particularly, fibers that require sterilization, such as roll towels for cabinets, Sheets, fukin, underwear,
The present invention relates to a method for determining the number of bacteria in handkerchiefs and wide cloths, and the like, and more particularly to a method for easily determining the number of bacteria in fibers without destroying the form of textile products.

(Prior art)

Recently, toilets and washrooms have been provided with roll towels for hand wiping housed in cabinets to provide users with comfort and convenience. These roll towels are usually operated under a lease system, and used towels are collected, cleaned,
After being bleached or sterilized, washed and dried, it is wound up and shipped to the consumer.

However, even though the conventional roll towels can sufficiently satisfy the cleanliness in appearance, they have not been sufficiently satisfactory from the viewpoint of microbiological cleanliness.
In particular, in the case of roll towels used for medical care and dining, etc., even the adhesion of sterilization may be a problem, but conventional roll towels do not provide any guarantee for such applications. Was.

Therefore, such roll towels and the like have recently been subjected to treatment such as disinfecting and cleaning, and have been shipped to consumers and gained a favorable reception. Further, such disinfecting cleaning is used not only for roll towels but also for sheets, fukins, underwear, handkerchiefs, wide cloths, and the like, and the quality of these is improved.

[Problems to be solved by the invention]

By the way, when such a roll towel or the like is sterilized, its quality cannot be guaranteed unless the sterilization state is inspected. Conventionally, such products are inspected for sterility by examining the number of bacteria by the Stomacher method. However, in the conventional method, a part of fibers such as a roll towel is cut off to make a test piece, which means the destruction or breakage of the fiber product. However, there is a problem in that the operation method is complicated, and such an operation method is complicated.

SUMMARY OF THE INVENTION An object of the present invention is to provide a simple method for testing the number of bacteria of fibers, which can easily perform the bacteria count of fibers without causing damage to a part of the fibers.

It is another object of the present invention to provide a simple bacterial count test method that can directly perform a required partial test on textile products.

[Means for solving the problem]

The simple bacterial count method for fibers of the present invention comprises a container containing sterilized water and a lid having a projection protruding toward the inside of the container. The fibers of the area are sandwiched between the fibers taken into the container and the inner wall of the container and between the fibers and the lid so that a space is secured, and sterile water is allowed to exist between both spaces to remove the fibers. The method is characterized in that the sandwiched container and lid are shaken to extract the fungi in the fibers into sterilized water to produce a bacterial solution.

[Action]

The present invention is intended to extract fungi into sterilized water by shaking the object to be inspected between a container containing the extracted sterilized water and a lid thereof, and shaking the fibers. This is based on the finding that when the projections are provided, fungi in the fiber are extracted very well into sterilized water.

When performing the bacterial count test according to the method of the present invention, a part of the fiber to be inspected is sandwiched between the container and the lid, and a part thereof is sealed in the container. It will not be cut off. The fibers in the container come into contact with sterilized water by shaking the container, and the attached fungi are extracted. In this case, fungi cannot be sufficiently extracted from the fibers to be inspected simply by inserting a part of the fibers. Therefore, in the present invention, a projection is provided on the lid, and the projection is formed to have a length at least immersed in sterile water added to the container. Thereby, the fibers are pushed down deeply in the container, at least a part thereof is immersed in the sterilized water, and the setting is made such that the sterilized water exists in both the space on the lid body side and the container inner wall side bordering the fibers. Things.

When the protrusions having such a length are provided, fungi can be well extracted from the fibers. In such a simple inspection method, a clear correlation was found between the stomacher method, which is the current method, and an order of 10 to 10 ⁵ per 1 cm ^{2 of the} fiber, as shown in Examples described later.

〔Example〕

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a simplified method for measuring the number of bacteria of fibers according to the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is an explanatory view of a container in which the simple bacterial cell count method for fibers according to the present invention is used. Container 2 as shown in FIG.
Inside, sterilized water (extracted water) 4 is added to 1/3 to 1/2. The lid 6 is fitted to the container 2 with the fibers 8 interposed therebetween, and a protrusion 10 is provided at a substantially central portion of the inner surface of the lid 6 so as to project into the container 2.

In such a configuration, when the container 2 is shaken, fungi are extracted from the fibers 8 into the sterilized water. There is no problem with shaking of the container 2 with a shaking device or a manual strong shake, and the number of times of shaking of the container 2 is at least 20 times, more preferably
FIG. 2 is a graph showing the number of bacteria extracted per 1 cm ² of fibers and the number of times of shaking, in the range of 50 to 100 times.
As shown in FIG. 2, it can be seen that the number of bacteria extracted from the fibers becomes substantially constant after 20 or more shakings. Table 1 shows the relationship between the number of extracted bacteria (per 1 cm ² ) and the logarithm of the log and the number of times of shaking for three samples of fibers. As is clear from this table, the number of times of shaking is constant at 50 to 100 times in the simple bacteria count method for fibers of the present invention. Incidentally, the shaking method of the present invention is a general one,
Even if the shaking method is changed, it is expected that certain bacteria are extracted from the fibers by a certain degree of shaking. Table 1 shows the results.

The length of the projection 10 of the lid 6 is preferably at least 1/4 of the length of the container 2. For example, as shown in FIG. 1, sterilized water 4 exists in the space 12 on the lid 6 side and the space 14 on the container inner wall side. When the protrusions 10 are formed to have such a length, fungi can be favorably extracted in addition to the fibers.

The height of the container 2 of the embodiment shown in FIG.
mm, and the protrusion 10 is 24 mm. In comparison with this example, the one without the projection, the one with the projection only 6 mm, and the one without the projection as shown in Table 2 and the one with only 6 mm as shown in Table 2 Failed to extract fungi sufficiently.

The simple bacterial cell count method for such fibers was applied to 50 samples having different numbers of adhered cells, and the exact cell count of each sample was determined by the current method, the Stomacher method. The graph of FIG. 3 shows the correlation between the simple bacterial water test method for fibers and the Stomacher method for these 50 samples. According to this result, a simple cell count tests fiber although detection sensitivity compared to current stomacher method one order lower, the correlation between the current stomacher method was observed in the range of up to 10 to 10 ^5. Lo
The correlation formula determined by taking the logarithm was log F = 1.02 Log S−1.07 R = 0.933 (F: number of bacteria by this method, S: number of bacteria by Stomacher method).

FIG. 4 is an explanatory diagram of each part of the underwear to which the simple inspection method of fibers according to the present invention is applied. Underwear 16 shown in FIG.
Is a sample that has been subjected to EOG sterilization and worn by 10 different people to check the results of this test method. As shown in the figure, the application points of the simplified bacterial cell count method for fibers are on both sides 16A and 16B, behind the neck 16C, and before the abdomen 16D. Table 3 shows the results.

From this result, the difference in the number of bacteria depending on the sample appears clearly,
It can be seen that the simple bacterial count method for fibers of the present invention is also suitable for a partial bacterial test.

From the above, the simple bacterial cell count method for fibers is a simple test method that can replace the current method of the stomacher method.

〔The invention's effect〕

As described above, the simple bacterial count method for fibers of the present invention uses the lid when the fibers to be inspected are shaken between the container containing the extraction sterilized water and the lid. Since the projections are provided on the body, fungi in the fibers can be satisfactorily extracted into sterilized water. Further, in the method of the present invention, which was examined using the extract thus extracted, a clear correlation with the current method, the Stomacher method, on the order of 10 to 10 ⁵ is seen. For this reason, it is possible to accurately test the number of bacteria such as a roll towel for a cabinet that requires a bacteria test of fibers by a simple operation and without damaging the fibers.

[Brief description of the drawings]

FIG. 1 is a cross-sectional view of a test container using the simple bacterial cell count method for fibers according to the present invention, FIG. 2 is a graph showing the number of shakes with the number of cells extracted from 1 cm ² of fibers, FIG. 3 is a correlation graph between the method of the present invention and the current Stomacker method. FIG. 4 is an explanatory diagram of each part of the underwear to which the simple inspection method of fibers according to the present invention is applied. 2 ... container, 4 ... sterile water, 6 ... lid, 8 ... fiber, 10 ... projection, 12 ... lid-side space, 14 ... container-side space, 16 ... underwear.

Claims (1)

(57) [Claims] 1. A container containing sterilized water and a lid having a projection protruding into the container allow a part of the fibers to be inspected to be contained in the container with a certain area of fibers. Hold the container so that the space between the taken fiber and the inner wall of the container and the space between the fiber and the lid are secured, and allow sterile water to exist between both spaces. Extracting a fungus in the fiber into sterilized water, and examining the number of bacteria in the extract.