JP2905725B2 - Food composition derived from tomato - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a tomato-derived food composition, and more particularly, to a tomato-derived food composition having an action of suppressing an increase in blood pressure and maintaining good tomato flavor.

[0002]

2. Description of the Related Art In recent years, it has been reported that the relationship between hypertension and the renin-angiotensin system at the gene level has been reported. One of the causes of hypertension is the effect of angiotensin converting enzyme (hereinafter abbreviated as "ACE"). Anomalies came to be mentioned. ACE is an enzyme that is mainly involved in two blood pressure regulating systems and exhibits a blood pressure effect. It is said that abnormal action of this enzyme induces hypertension. Therefore, attempts have been made to improve hypertension by administering a substance that inhibits the pressor action of ACE. At present, ACE inhibitors have already been used clinically for the treatment of hypertension. As there are no noticeable side effects, the proportion of all antihypertensive drugs is increasing.

[0003] On the other hand, elucidation of bioregulatory functions as a tertiary function of foods has been made for various foods, and in the process, many physiologically active substances have been found in foods and their enzyme digests. There have been many reports of isolating ACE inhibitors as described above, probably because of the simplicity of the assay method. In addition, among ACE inhibitors obtained from such foods and the like, some of them have been confirmed to be effective when administered to model animals of hypertension, and the development of functional foods utilizing such characteristics has been developed. Has also been made.

[0004] However, it is not known that tomato contains a substance having an ACE inhibitory action, and it has been reported that an ingredient containing an ACE inhibitory substance was extracted from tomato and used as a functional food for improving hypertension. Nor.

[0005] In recent years, some functional foods derived from tomato have been researched and developed, and foods having a function of suppressing an increase in blood pressure as a function thereof have been developed. For example, Japanese Unexamined Patent Publication
This is the functional food derived from tomato described in JP-A-224467. However, the above-mentioned invention is characterized in that glutamic acid decarboxylase is allowed to act on the processed ripe tomato to convert a part of the glutamic acid contained in the ripe tomato into γ-aminobutyric acid having a blood pressure increase inhibitory effect,
It has the effect of suppressing blood pressure increase by increasing the content of aminobutyric acid, and there is a substance having the ACE inhibitory effect in the tomato's original components, which indicates that it is involved in the blood pressure increase suppressing effect. There is no description. Also, in the present invention, instead of increasing the content of γ-aminobutyric acid, the content of glutamic acid that produces a good flavor of tomato is reduced, so that the resulting functional food has good tomato flavor. There was a problem that it was not enough. Therefore, development of a tomato-derived food composition having an action of suppressing an increase in blood pressure while maintaining good flavor of tomato has been desired.

[0006]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above, and an object of the present invention is to provide a food composition derived from tomato, which has an action of suppressing an increase in blood pressure and maintains a good flavor of tomato. Make it an issue.

[0007]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above problems, and as a result, have found that a substance having an ACE inhibitory action is present in tomato fruits.
Serum of tomato juice and / or a concentrate thereof are obtained while maintaining a good flavor of tomato, and these have an effect of actually suppressing an increase in blood pressure. Furthermore, molecular weight obtained from serum of tomato juice The present inventors have found that a water-soluble compound of 4000 or less contains an ACE inhibitor in a higher concentration and is excellent in an effect of suppressing an increase in blood pressure, thereby completing the present invention.

[0008] That is, the present invention relates to a food composition having a blood pressure increase-inhibiting action containing a serum of tomato juice and / or a concentrate thereof as an active ingredient, and a water-soluble compound having a molecular weight of 4000 or less contained in the serum of tomato juice. Is a food composition having an effect of suppressing an increase in blood pressure, which comprises the following as an active ingredient:

Hereinafter, the present invention will be described in detail. The food composition having an effect of suppressing blood pressure elevation according to the present invention contains a serum of tomato juice and / or a concentrate thereof as an active ingredient. Alternatively, the molecular weight 4 contained in the serum of tomato juice
Contains no more than 000 water-soluble compounds as active ingredients.
In tomato fruit, there is a substance having an ACE inhibitory action, and if this is administered to a person with hypertension, for example,
It is presumed that this substance inhibits the ACE pressor action in the body and improves hypertension. Therefore, ripe raw tomato itself, or a finely ground one,
Tomato juice and the like also have a blood pressure increase inhibitory action by the ACE inhibitor, but in order to effectively bring out the blood pressure increase inhibitory action, a serum containing the ACE inhibitor at a high concentration is removed from the tomato juice by centrifugation or the like. It is preferable that the concentrate is further concentrated, if necessary, and used as an active ingredient of the food composition of the present invention. In addition, a water-soluble compound having a molecular weight of 4000 or less is extracted from the serum of the tomato juice as a component containing a higher concentration of an ACE inhibitor, and used as an active ingredient of the food composition having a blood pressure increase inhibitory effect of the present invention. Is also possible.

[0010] Here, a method of obtaining the serum of the tomato juice containing the ACE inhibitor from the tomato fruit is as follows.
Obtain tomato juice from tomato fruit according to the usual method.
As a specific method of obtaining a juice, the washed and sorted raw tomatoes are crushed using a crusher or the like, heated with a tube heater or the like to sterilize and deactivate enzymes, and then extracted using an extractor or the like. For example, a method of squeezing or squeezing using a pulper / finisher or the like can be used.

Next, as a method for obtaining a serum containing an ACE inhibitor from the above-mentioned juice, a method of separating the above-mentioned juice into a high-molecular-weight precipitate and a serum by a centrifugal separator is suitable. The centrifugation conditions are 1000-15000G, preferably 10,000-15000G, and the centrifugation time is 8-12.
Minutes are preferred. Further, if necessary, the tomato serum obtained in this way is concentrated by heating or concentrated by a reverse osmosis membrane, so that a tomato serum concentrate having a high ACE inhibitor-containing concentration can be obtained. here,
The concentration ratio can be appropriately selected in consideration of, for example, the dosage form of the composition containing the compound. For example, when a concentrate of tomato juice serum is used for food or drink, the concentration ratio is preferably 2 to 8 times, and more preferably 4 to 6 times. If the concentration ratio is small, the blood pressure increasing effect may not be sufficient, and if the concentration ratio is too large, the acidity becomes strong and the taste of the food or drink may be impaired.

As a method for extracting a water-soluble compound having a higher molecular weight of 4000 or less and containing an ACE inhibitor at a higher concentration from the tomato serum, a dialysis membrane having an exclusion limit molecular weight of about 4000, for example, a spectrum pore / 3 (manufactured by Spectrum Medical Industry)
For example, a method of performing dialysis for about 12 to 24 hours against 5 to 20 times the amount of water using the method described above. Since the water-soluble compound having a molecular weight of 4000 or less is contained in the external dialysis solution, the external dialysis solution can be added to the food composition of the present invention after the treatment such as concentration under reduced pressure. (Concentration) A dialysate obtained by desalting by gel filtration column chromatography or the like, and a concentrate obtained by concentrating the dialysate under reduced pressure can be added to the food composition of the present invention.

[0013] The serum of the tomato juice and / or its concentrate obtained in this way contains an ACE inhibitor at a higher concentration than tomato fruits and tomato juice.
In addition, the water-soluble compound having a molecular weight of 4000 or less contained in the serum of the tomato juice contains a higher concentration of the ACE inhibitor than the serum of the tomato juice and / or its concentrate. Furthermore, since the flavor of the tomatoes is not impaired in the process of producing them, the resulting serum of the tomato juice and / or its concentrate, or the water-soluble compound having a molecular weight of 4000 or less contained in the serum of the tomato juice is , With a good tomato flavor.

The food composition of the present invention contains a water-soluble compound having a molecular weight of 4000 or less contained in the serum of the tomato juice and / or a concentrate thereof, or the serum of the tomato juice. It has an effect of suppressing an increase in blood pressure by an ACE inhibitor and sufficiently retains a good flavor of tomato.

When the food composition of the present invention is mixed with a water-soluble compound having a molecular weight of 4000 or less contained in such a serum of tomato juice and / or a concentrate thereof or a serum of tomato juice, , Can be blended with optional components commonly used in foods. Examples include confectionery such as crackers, cakes, cookies, and jellies, drinks such as juices, vegetable drinks, alcoholic drinks, staple foods such as bread, sauces, and seasonings such as ketchup.

Further, the food composition of the present invention comprises a health food,
Various forms usually used as health drinks, for example,
It also includes powders, granules, tablets, capsules, liquids, etc., and in formulating these, excipients, binders, disintegrants, lubricants, flavoring agents, bulking agents, coating agents, etc. Normal,
It is possible to mix optional components used in the formulation of health foods and health drinks in an arbitrary amount, and these can be produced by the same method as that for producing the above-mentioned preparations in general.

[0017]

Embodiments of the present invention will be described below. (1) Tomato health drink (tomato serum, 8-fold concentrated tomato serum) Tomato juice was prepared according to a conventional method for producing tomato juice. That is, the washed and sorted raw tomatoes were crushed using a crusher, preheated to about 70 ° C. with a tube heater to sterilize and deactivate enzymes, and then squeezed using an extractor. Thereafter, the juice was heated at about 120 ° C. for about 50 seconds for sterilization, and a part of the obtained tomato juice was stored frozen for use in a blood pressure increase suppression test described below.

The tomato juice obtained above was added to 12
Centrifugation was performed at 000 G for 8 minutes to obtain a serum of the tomato juice. A part of the tomato serum was used as a healthy tomato beverage of the present invention, and a part thereof was frozen and stored for a blood pressure increase suppression test described below.

Next, the obtained tomato serum was concentrated by heating under reduced pressure to 8 times the concentration of soluble solids, and this was frozen and stored as another tomato health drink of the present invention. Moreover, the tomato health drink of the present invention comprising the tomato serum or the 8-fold concentrated solution obtained in this way sufficiently retained the flavor of tomato.

(2) Healthy Tomato Drink (Low Molecular Fraction of Tomato Serum) First, a serum of tomato juice was prepared in the same manner as in (1) above. The obtained tomato serum is added to the spectrum pore / 3
(Exclusion limit molecular weight: about 4000, manufactured by Spectrum Medical Industry) was used to dialyze against 9 times the volume of water overnight, and the dialysate was desalted by gel filtration chromatography. This solution was concentrated by heating under reduced pressure to obtain a water-soluble compound having a molecular weight of 4000 or less contained in the tomato serum as the healthy tomato beverage of the present invention. Further, the healthy tomato drink of the present invention comprising the low molecular weight fraction of the tomato serum had a sufficient tomato flavor.

The tomato juice obtained in the above (1), the tomato serum which is the tomato health drink of the present invention, an 8-fold concentrated solution of the tomato serum, and the tomato health drink of the present invention obtained in (2). Using a water-soluble compound having a molecular weight of 4,000 or less contained in tomato serum, the ACE inhibitory effect and the blood pressure increase suppressing effect were evaluated by the following methods.

[0022]

Example 1 ACE Inhibiting Effect The tomato-derived food composition of the present invention has an effect of inhibiting the ACE pressor action, thereby suppressing an increase in blood pressure. ACE is an enzyme mainly involved in two blood pressure regulating systems and exerting a blood pressure effect. One is the C-terminal dipeptide (His-Le) of angiotensin I (no pressor action) produced by renin from angiotensinogen.
u) to cleave a potent pressurizing peptide, angiotensin II, and another to cleave and inactivate the endogenous antihypertensive peptide, bradykinin. Experimental results showing that the derived component inhibits the system that produces angiotensin II from angiotensin I and inhibits the production of angiotensin II, which is a pressor peptide, thereby inhibiting the ACE's pressor action.

1) In vitro test The reagents used were 0.15 M borate buffer, a borate buffer containing ACE (manufactured by Sigma) as an enzyme solution at a concentration of 0.1 unit / mL, and a substrate. Borate buffer (containing 350 mM NaCl) containing (-)-hippuryl-L-histidyl-L-leucine n-hydrate (manufactured by Wako Pure Chemical Industries, Ltd.) at a concentration of 4.02 mg / mL as a solution. Met. The ACE inhibition sample used in the test was a water-soluble compound having a molecular weight of 4000 or less contained in the tomato serum obtained in (1) and the tomato serum obtained in (2).

First, 80 μL of the ACE inhibition sample, 20 μL of the enzyme solution, and 100 μL of the substrate solution were mixed in a test tube under ice cooling, and the enzyme reaction was carried out at 37 ° C. for 50 minutes. The reaction was stopped by adding 250 μL of 1N hydrochloric acid (ice-cooled) thereto, followed by adding 1.5 mL of ethyl acetate and stirring, followed by centrifugation (3000 rpm, 5 minutes) to separate the ethyl acetate layer for 1 hour.
Dispensed into mL test tubes. This was heated, and ethyl acetate was evaporated and dried, then dissolved in 1.25 mL of water, and the absorbance at 228 nm was measured.

The evaluation of the enzyme activity was carried out by calculating the rate of inhibition of the enzyme activity when a borate buffer was added instead of the ACE-inhibited sample. That is, the absorbance when the ACE inhibition sample was added was S, the value when the borate buffer was added instead of the AEC inhibition sample was C, and the value when the reaction was performed with the borate buffer instead of the enzyme solution was C. If B, the inhibition rate of the ACE-inhibited sample is derived by the following equation.

[0026]

## EQU1 ## Inhibition rate (%) = ((CS) / (CB)) * 100 The tomato serum obtained in the above (1) and the tomato obtained in the above (2), which are obtained by this equation. Molecular weight 4 contained in serum
Table 1 shows the in vitro ACE inhibitory activity of 000 or less water-soluble compounds (both final concentrations in the assay system).

[0027]

[Table 1]

From these results, it is clear that both the tomato serum obtained in the above (1) and the water-soluble compound having a molecular weight of 4000 or less contained in the tomato serum obtained in the above (2) are excellent in ACE inhibitory activity in vitro. I understood. In addition, the 50% inhibitory concentration of both samples showed that the ACE inhibitory activity of the water-soluble compound having a molecular weight of 4000 or less contained in tomato serum was 100 times or more stronger than that of tomato serum.

2) In Vivo Test As described above, ACE is an enzyme that converts inactive angiotensin I (hereinafter abbreviated as "ANGI") to ANGII which has a blood pressure increasing effect. Therefore, ANGI
Is intravenously converted to ANGII by ACE in the blood and increases blood pressure. However, if an ACE inhibitor is administered in advance, blood pressure will not increase even if ANGI is administered. This phenomenon was observed in rats (Wistar strain, body weight 30).
0 g, male (Shimizu experimental material)). ACE
As the inhibitor, a water-soluble compound having a molecular weight of 4000 or less contained in the tomato serum obtained in the above (2) was used.

A specific experimental method will be described. First, 200 μL of physiological saline is administered to rats, and it is confirmed that the blood pressure does not change at this time.
0-150 ng / kg). The blood pressure increase value at this time serves as a control, but a 10-20 mmHg increase is desirable, and this value was used as a pre-control. Then AC
E inhibitor was administered at 150 μL, and ANGI (100
１５０150 ng / kg). The blood pressure increase value at this time was used as a trial and compared with the blood pressure increase when ANGI was first administered. If the ACE inhibitor is acting, this trial will be smaller than the pre-control. Thereafter, ANGI (100-150 ng /
kg), and the blood pressure increase value at this time was used as post control. At this time, if the ACE inhibitor has been metabolized, it is considered that the blood pressure elevation value at this time is almost the same as the blood pressure elevation value when ANGI was first administered.

In the above experiment, the blood pressure of the rat was measured under anesthesia (urethane anesthesia, 1.0 to 1.5 g / kg).
And a catheter was inserted into the left carotid artery, and the pulse pressure in the blood vessel was continuously measured via a pressure transducer (DT-12, manufactured by Vigo Spectrum). Also, AN
The administration method of the GI or ACE inhibitor is to administer from a catheter inserted into the femoral vein, and the administration rate is 100 μL / mi.
n, and the single dose was kept at 200 μL or less.

This experiment was performed five times, and the pre-control
The average value of the blood pressure elevation values obtained as a trial and post control was determined. From these values, the relative value of the other blood pressure increase average value when the average value of the pre-control was set to 100 was calculated. Table 2 shows the results.

[0033]

[Table 2]

As is clear from these results, the blood pressure increase value of the trial is about 60% lower than the blood pressure increase values of the pre-control and the post-control, and therefore, the molecular weight contained in the tomato serum obtained in the above (2) 4000
The following water-soluble compounds were also found to be excellent in ACE inhibitory activity in vivo.

[0035]

Example 2 Blood Pressure Elevation Suppressing Action Naturally developed 4-fold concentrated tomato serum (the tomato health drink of the present invention) obtained by diluting the tomato juice and 8-fold concentrated tomato serum obtained in (1) above with an equal amount of water. It was administered to rats with essential hypertension to evaluate its effect. The spontaneously hypertensive rats used for the evaluation are generally used as model animals for hypertension, develop onset at 5 to 6 weeks of age, and begin to increase in blood pressure.
It is known to reach equilibrium at 2-14 weeks of age.

Four-week-old spontaneously hypertensive rats (SH
R) After purchasing 21 animals (Hoshino test animal breeding station) and carrying out preliminary breeding for 2 weeks, systolic blood pressure (systolic blood pressure, hereinafter referred to as "S
BP]), and the animals were divided into three groups of seven each such that the average value was almost the same between the groups (the average of the body weights was also made as uniform as possible).

During preliminary breeding, water was freely taken as a drink, but after grouping, two of the three groups contained:
The tomato juice obtained in the above (1) and the 4-fold concentrated tomato serum obtained by diluting the 8-fold concentrated tomato serum with an equal amount of water were provided as drinks, and the remaining group was provided with water as a control group as water. . All of these drinks were allowed to be taken freely, and the amount of intake was measured by the weight of a water bottle. Also, the feed is
The MF solid feed of Oriental Yeast Co., Ltd. was allowed to freely ingest from pre-breeding to the end of the test. During the pre-breeding period, the animals were bred in four cages per cage, and after grouping, they were bred in two cages per cage. The temperature of the breeding room was kept at 25 ° C., and the day and night were cycled for 12 hours (lighted on from 6:00 to 18:00). The test was performed for 6 weeks after the completion of the preliminary breeding. The amount of the tomato juice or the four-fold concentrated solution of the tomato juice serum that the SHR ingested as described above was about 30 g per animal per day.

While bred as described above, the blood pressure of SHR was measured once a week by a non-invasive method. What is the non-invasive method?
This is a method of measuring blood pressure by detecting blood flow in the tail artery of a rat with an optical sensor. That is, the tail artery is gradually pressurized with a cuff, and the pressure at which blood flow cannot be detected is measured. The pressure at this time is defined as SBP. Specifically, 50 ° C
The SHR is placed in a stainless steel breeding cage set at about 5 mm on a hot plate heated for 15 minutes and heated (this heating allows blood to flow sufficiently to the blood vessels of the tail, which is also a heat radiating organ), and after heating , Immediately in gloves S
The HR was restrained and the blood pressure (SB) was measured using a blood pressure measurement device (KN-210 rat tail artery pressure, pulse measurement device; Natsume Seisakusho Co., Ltd.).
P) was measured. The measurement was continuously performed 5 to 6 times per animal, and the average excluding the first time (in some cases, the first and second times) was taken as the SBP of the SHR. Here, the reason why the first measurement value is rejected is that the first measurement value often shows an extremely high value as compared with the subsequent blood pressure.

The average value and standard deviation of the SBP of 7 animals per group were determined, and a significant difference was observed between the control group, the group to which the tomato juice was administered, and the group to which the 4 times concentrated tomato juice was administered. It was tested by the Student's-T method. The results are shown in Table 3 and FIG.

The symbol * in FIG. 1 indicates that the data is significant at a risk rate of 5% or less as compared with the control group.

[0041]

[Table 3]

From these results, the SHR group to which the tomato juice obtained in the above-mentioned embodiment (1) was administered, the 4-fold concentration of the 8-fold concentrated tomato serum obtained in (1) diluted with an equal amount of water In both SHR groups to which the concentrated tomato serum (the tomato health drink of the present invention) was administered, it can be judged that the increase in blood pressure is generally suppressed compared to the control group which did not receive these administrations. The effect of the sap on the increase in blood pressure against SHR is not sufficient, whereas the SHR administered with 4-fold concentrated tomato serum, which is the tomato healthy beverage of the present invention,
In the group, the increase in blood pressure was well suppressed, indicating that the healthy tomato beverage of the present invention has a sufficient blood pressure increase suppressing effect.

[0043]

EFFECTS OF THE INVENTION The tomato-derived food composition of the present invention is excellent in the action of maintaining high flavor of tomatoes and suppressing ACE's vasopressor action to suppress an increase in blood pressure. It is promising as a functional food for improving diseases and the like.

[Brief description of the drawings]

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the change over time in the systolic blood pressure (SBP) of the SHR group to which the tomato juice was administered, the SHR group to which a 4-fold concentrated solution of the tomato juice was administered, and the control SHR group.

──────────────────────────────────────────────────続 き Continued on the front page (58) Fields surveyed (Int. Cl. ⁶ , DB name) A23L 1/05-1/09 A23L 1/30 JICST file (JOIS)

Claims (2)

(57) [Claims] 1. A food composition having an effect of suppressing blood pressure elevation, comprising a serum of tomato juice and / or a concentrate thereof as an active ingredient. 2. Molecular weight 4 contained in serum of tomato juice
A food composition having an effect of suppressing blood pressure elevation, comprising a water-soluble compound of 000 or less as an active ingredient.