JP2958367B2 - Tissue fluid suction sampling device - Google Patents
Tissue fluid suction sampling deviceInfo
- Publication number
- JP2958367B2 JP2958367B2 JP2259573A JP25957390A JP2958367B2 JP 2958367 B2 JP2958367 B2 JP 2958367B2 JP 2259573 A JP2259573 A JP 2259573A JP 25957390 A JP25957390 A JP 25957390A JP 2958367 B2 JP2958367 B2 JP 2958367B2
- Authority
- JP
- Japan
- Prior art keywords
- tissue fluid
- suction
- pressure
- cell
- fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012530 fluid Substances 0.000 title claims description 69
- 238000005070 sampling Methods 0.000 title claims description 21
- 210000001519 tissue Anatomy 0.000 description 49
- 239000008280 blood Substances 0.000 description 15
- 210000004369 blood Anatomy 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 12
- 239000008103 glucose Substances 0.000 description 12
- 239000007788 liquid Substances 0.000 description 12
- 210000003491 skin Anatomy 0.000 description 10
- 238000010586 diagram Methods 0.000 description 5
- 239000003085 diluting agent Substances 0.000 description 5
- 210000002615 epidermis Anatomy 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 210000000434 stratum corneum Anatomy 0.000 description 4
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 210000005056 cell body Anatomy 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は無侵襲的に血糖値を測定出来る血糖値測定装
置等に用いて好適な組織液吸引採取装置に関する。Description: TECHNICAL FIELD The present invention relates to a tissue fluid suction and sampling device suitable for use in a blood sugar level measuring device or the like capable of noninvasively measuring a blood sugar level.
本発明は無侵襲的に血糖値を測定出来る血糖値測定装
置等に用いて好適な組織液吸引採取装置に関し、人体等
の被検体の皮膚に吸引セルを密着させ、吸引セル内を減
圧して組織液を採取する様にした組織液吸引採取装置の
於いて、吸引セルを吸引手段を介して吸引し、組織液を
定量採取する定量採取手段と、定量採取手段内の気圧を
大気に開放する大気開放手段又は気圧を増圧する気圧増
圧手段とを具備させることで気泡の影響を与えることな
く正確に定量の組織液を採取する様にしたものである。The present invention relates to a tissue fluid suction and sampling device suitable for use in a blood sugar level measuring device or the like capable of noninvasively measuring a blood sugar level. In a tissue fluid suction and sampling device configured to sample blood, a suction cell is suctioned through a suction means, and a quantitative sampling means for quantitatively sampling the tissue fluid, and an atmosphere opening means for releasing the pressure in the quantitative sampling means to the atmosphere or By providing a pressure increasing means for increasing the atmospheric pressure, a fixed amount of tissue fluid can be accurately collected without the influence of air bubbles.
従来から、血液採取を行うことなく、表皮角質層を除
去した後に、表皮に吸引装置の吸引セルを密着させ、減
圧吸引することで表皮上に浸出される微量な吸引浸出液
（以下組織液と記す）を検出し、この組織液に基づい
て、経皮的に糖濃度を測定出来る血糖値測定装置が例え
ば、電子情報通信学会誌、1989年４月21日、MBE89−1
1、「経皮的吸引浸出液取付法とISFET型バイオセンサを
用いた無侵襲連続血糖値測定システム」の論文に開示さ
れている。Conventionally, without removing blood, after removing the stratum corneum of the epidermis, a suction cell of a suction device is brought into close contact with the epidermis, and a small amount of suction exudate leached onto the epidermis by suction under reduced pressure (hereinafter referred to as tissue fluid). A blood glucose measurement device capable of detecting glucose concentration and percutaneously measuring glucose concentration based on this tissue fluid is described in, for example, IEICE, April 21, 1989, MBE89-1.
1. It is disclosed in the paper of "Non-invasive continuous blood glucose measurement system using percutaneous suction leachate attachment method and ISFET type biosensor".
この様な血糖値測定装置では採取した組織液のグルコ
ース（GLUCOSE C6H12O6）値を測定することで血糖値を
測定するものである。この血糖値測定装置は、例えば、
被検者の皮膚の一部の角質層を除去して表皮を露出さ
せ、ここに吸引セルを対接させ、吸引ポンプ等を介して
吸引すると組織液が浸出する。この組織液を採取してグ
ルコース値を測定している。この様な血糖値測定装置の
組織液吸引採取装置を本出願人は第４図に示す様に構成
されていた。第４図で皮膚（11）の角質層（11a）はス
トリッピング処理で剥離し、吸引セル（１）のセル本体
（２）を皮膚（11）上に密着させる。セル本体（２）は
チューブ（8a）を介して切換弁（12）の一端に接続さ
れ、他端はチューブ（8a）を介して圧力ゲージ（13）並
に吸引ポンプ（14）に結合されている。更に希釈液槽
（15）に入れられた希釈液（16）を送液ポンプ（17）と
チューブ（8b）を介して切換弁（12）に供給し、切換弁
（12）を切換えて、希釈、撹拌部（18a）にチューブ（8
c）を介して組織液及び希釈液（16）を供給して、希
釈、撹拌させる。この様に希釈、撹拌した液を測定部
（18）にチューブ（8d）を介して供給し、校正測定表示
記録部（19）で、校正した測定値を表示或は記録する。
測定部（18）で測定された廃液（21）はチューブ（8e）
を介してドレイン槽（20）に排出され、測定部（18）、
希釈、撹拌部（18a）等は洗浄液槽（24）内の洗浄液（2
3）で送液ポンプ（22）及びチューブ（8f）及び（8d）
を介して洗浄される様に成されている。Such a blood sugar level measuring device measures a blood sugar level by measuring a glucose (GLUCOSE C 6 H 12 O 6 ) value of a collected tissue fluid. This blood glucose measuring device is, for example,
When a part of the stratum corneum of the subject's skin is removed to expose the epidermis, a suction cell is brought into contact therewith, and when suction is performed using a suction pump or the like, tissue fluid is leached. The tissue fluid is collected and the glucose level is measured. The applicant of the present invention has such a blood sugar level measuring apparatus as shown in FIG. In FIG. 4, the stratum corneum (11a) of the skin (11) is peeled off by stripping, and the cell body (2) of the suction cell (1) is brought into close contact with the skin (11). The cell body (2) is connected to one end of a switching valve (12) via a tube (8a), and the other end is connected to a pressure gauge (13) and a suction pump (14) via a tube (8a). I have. Further, the diluting liquid (16) put in the diluting liquid tank (15) is supplied to the switching valve (12) via the liquid sending pump (17) and the tube (8b), and the switching valve (12) is switched to dilute. , A tube (8
Supply the tissue fluid and diluent (16) via c) to dilute and stir. The diluted and stirred liquid is supplied to the measuring section (18) via the tube (8d), and the calibrated measured value is displayed or recorded in the calibrated measurement display recording section (19).
The waste liquid (21) measured by the measuring section (18) is a tube (8e)
Is discharged to the drain tank (20) through the measuring unit (18),
The dilution / stirring unit (18a) is used for cleaning liquid (2
In 3), feed pump (22) and tubes (8f) and (8d)
It is made to be washed through.
上述のシステム構成に於ける切換弁の構成を第５図A,
Bに示す。第５図A,Bは切換弁（12）の縦断面図であり、
四方弁構造と成されている。第５図A,Bに於いて、（3
0）は弁本体であり、回転スプール（31）の弁棒（32）
が弁本体（30）内で回動出来る様に成され、弁棒（32）
は回転角度90゜間隔でクリック的に弁本体（30）に対し
係止出来る様に成されている。弁本体（30）の左右開口
（33L）（33R）は送液ポンプ（17）からのチューブ（8
b）及び希釈、撹拌部（18a）に連なるチューブ（8c）に
結合され、上下開口（33U）（33D）は圧力ゲージ（13）
に連なるチューブ（8a）及び吸引セル（１）に連なる同
じくチューブ（8a）に結合されている。更に弁棒（32）
内には上下、左右開口（33U）（33D），（33L）（33R）
に連なる液路（34）が形成されている。後述するも弁棒
（32）を手動等で回転させれば、第５図Ａの状態から、
第５図Ｂに示す状態と成って液路（34）は上下開口（33
U）（33D）から左右開口（33L）（33R）側に向けられ第
５図Ａの状態では吸引セル（１）より組織液（25）を液
路（34）及びチューブ（8a）を含む経路内に採取し、次
に切換弁（12）を切換えて、第５図Ｂの状態と成し、希
釈液槽（15）から送液ポンプ（17）を介して組織液（2
5）中に希釈液（16）が混合されて希釈撹拌部（18a）に
排出されることで組織液（25）は弁棒（32）の液路（3
4）で定まる容量の一定量が採取されてグルコース値の
測定が行なわれる様に成されている。The structure of the switching valve in the above system configuration is shown in FIG.
Shown in B. 5A and 5B are longitudinal sectional views of the switching valve (12),
It has a four-way valve structure. In FIGS. 5A and B, (3
0) is a valve body, and a valve stem (32) of a rotating spool (31).
Can be rotated in the valve body (30), and the valve stem (32)
Is designed so that it can be clicked on the valve body (30) at intervals of a rotation angle of 90 °. The left and right openings (33L) (33R) of the valve body (30) are connected to the tube (8
b) and connected to the tube (8c) connected to the dilution / stirring unit (18a), and the upper and lower openings (33U) (33D) are pressure gauges (13)
And a tube (8a) connected to the suction cell (1). Further valve stem (32)
Inside the top and bottom, left and right opening (33U) (33D), (33L) (33R)
A liquid path (34) is formed. As will be described later, if the valve stem (32) is rotated manually or the like, the state shown in FIG.
In the state shown in FIG. 5B, the liquid path (34) is opened vertically (33
U) From (33D), the tissue fluid (25) is directed from the suction cell (1) to the left and right openings (33L) (33R) in the path including the fluid path (34) and the tube (8a) in the state of FIG. 5A. Then, the switching valve (12) is switched to achieve the state shown in FIG. 5B, and the tissue fluid (2) is supplied from the diluent tank (15) via the liquid supply pump (17).
The tissue fluid (25) is mixed with the diluent (16) and discharged into the dilution stirrer (18a), so that the tissue fluid (25) passes through the fluid path (3)
A certain amount of the volume determined in 4) is collected and the glucose value is measured.
上述の従来構成で説明した切換弁（12）によると、弁
棒（32）の液路（34）に採取される組織液（25）内に含
まれる微小な気泡は膨張し目視で確認出来る程の気泡と
なる。これは吸引ポンプ（14）によって皮膚（11）から
吸引セル（１）を介して採取する際に400mmHg程度に減
圧されるため発生するもので、ボイル−シャルルの法則
の則とったものである。即ち、ある気体の絶対温度T1と
し、圧力がP1の時の体積がV1であるときに絶対温度が
T2、圧力P2、体積がV2になったとすれば の関係にあることから絶対温度T1T2が一定なら組織液
（25）中に含まれる空気の気泡は膨張して大きくなって
いることになる。According to the switching valve (12) described in the above-described conventional configuration, the minute air bubbles contained in the tissue fluid (25) collected in the fluid path (34) of the valve stem (32) are expanded to such an extent that they can be visually confirmed. It becomes bubbles. This occurs because the pressure is reduced to about 400 mmHg when the blood is collected from the skin (11) through the suction cell (1) by the suction pump (14), and follows the Boyle-Charles law. That is, the absolute temperature T 1 of the certain gases, is the absolute temperature when the pressure is volume V 1 of the case of P 1
Assuming that T 2 , pressure P 2 , and volume are V 2 Therefore, if the absolute temperature T 1 T 2 is constant, the air bubbles contained in the tissue fluid (25) expand and become larger.
また、組織液（25）中には溶存気体が存在し、この溶
存気体が吸引時（減圧時）に気泡として発生する。これ
はヘンリーの法則に従うもので、即ち大気圧における気
体の溶解度をＣ、大気圧をＰとすると、 Ｃ＝kP ‥‥（２） となり、減圧時における溶解度をC1減圧値をP1とすれば C1＝ｋ（Ｐ−P1） ‥‥（３） で表わせる。（２），（３）式より減圧時C1は C1＝Ｃ（１−P1/P） ‥‥（４） となる。（３）式より、Ｐ＞P1（P,P2は正の数）である
から減圧時の溶解度C1は大気圧時の溶解度Ｃよりも小、
即ちC1＜Ｃとなるので、減圧時に溶存していた気体が組
織液（25）中に気泡として析出することになる。In addition, a dissolved gas is present in the tissue fluid (25), and the dissolved gas is generated as air bubbles at the time of suction (at the time of pressure reduction). This follows Henry's law, that is, if the solubility of a gas at atmospheric pressure is C and the atmospheric pressure is P, then C = kP ‥‥ (2), and the solubility at reduced pressure is C 1 and the reduced pressure value is P 1. For example, C 1 = k (P−P 1 ) ‥‥ (3). From the expressions (2) and (3), C 1 at the time of pressure reduction is expressed as C 1 = C (1−P 1 / P) ‥‥ (4) From equation (3), since P> P 1 (P and P 2 are positive numbers), the solubility C 1 at reduced pressure is smaller than the solubility C at atmospheric pressure.
That is, since C 1 <C, the gas dissolved during the depressurization is deposited as bubbles in the tissue fluid (25).
この様な気泡（35）が所定容積内の弁棒（32）の液路
（34）内に存在すれば組織液（25）の絶対量は減少し、
定量採取することが出来なくなる。即ち、気泡の容積分
だけ定量採取時の精度に誤差を発生し、その結果、グル
コース測定値に大きな影響を与え、例えばインシュリン
の投与を行なっている糖尿病患者等を危険な状況に至ら
しめる弊害を生ずる。If such a bubble (35) exists in the fluid path (34) of the valve stem (32) within a predetermined volume, the absolute amount of the tissue fluid (25) decreases,
Quantitative sampling cannot be performed. That is, an error occurs in the accuracy at the time of quantitative sampling by an amount corresponding to the volume of the air bubbles, and as a result, a large effect is exerted on the glucose measurement value, for example, a harmful situation that causes a diabetic patient or the like who is administering insulin to a dangerous situation. Occurs.
本発明は叙上の問題点を除去した組織液吸引採取装置
を得ることを目的とするもので、気泡の影響なしに組織
液を正確に定量採取する様にしたものである。SUMMARY OF THE INVENTION An object of the present invention is to provide a tissue fluid suction and sampling apparatus which eliminates the above-mentioned problems, and is intended to accurately and quantitatively sample tissue fluid without the influence of air bubbles.
本発明の組織吸引採取装置はその１例が第１図に示め
されている様に、人体等の被検体の皮膚（11）に吸引セ
ル（１）を密着させ、この吸引セル（１）内を減圧して
組織液（25）を採取する様にした組織液吸引採取装置に
於いて、吸引セル（１）を吸引手段（14）を介して吸引
し、組織液（25）を定量採取する定量採取手段（12）
と、定量採取手段（12）内の気圧を大気に開放する大気
開放手段（36）又は気圧を増圧する増圧手段（37）とを
具備して成るものである。As shown in FIG. 1, one example of the tissue suction and sampling device of the present invention is that a suction cell (1) is brought into close contact with the skin (11) of a subject such as a human body, and this suction cell (1) In a tissue fluid suction and sampling device in which the inside is decompressed and a tissue fluid (25) is collected, the suction cell (1) is suctioned through the suction means (14) to quantitatively collect the tissue fluid (25). Means (12)
And an air release means (36) for releasing the pressure in the quantitative collection means (12) to the atmosphere or a pressure increasing means (37) for increasing the pressure.
本発明の組織液吸引採取装置は被検体の皮膚（11）か
ら組織液（25）を採取後に大気開放手段（36）又は増圧
手段（37）を切換えて、組織液（25）を大気中に曝す
か、或は積極的に大気圧よりも大きい圧力を組織液（2
5）に加えることで減圧下で膨張していた気泡を圧縮さ
せるので、組織液（25）の定量採取が可能となる。The tissue fluid aspirating and sampling apparatus of the present invention switches the atmosphere opening means (36) or the pressure increasing means (37) after collecting the tissue fluid (25) from the skin (11) of the subject to expose the tissue fluid (25) to the atmosphere. Or positively apply a pressure greater than atmospheric pressure to tissue fluid (2
By adding to (5), the bubbles expanded under reduced pressure are compressed, so that a fixed amount of tissue fluid (25) can be collected.
以下、本発明の組織液吸引採取装置を第１図乃至第３
図について詳記する。第１図は本発明の組織液吸引採取
装置の要部の等価図を示すもので、第４図との対応部分
には同一符号を付して示してある。同図で、吸引手段
（14）を構成する吸引ポンプ（14a）はモータ（14b）で
駆動され、チューブ（8a）内の圧力を減圧する。吸引ポ
ンプ（14a）の吸込口はチューブ（8a）を介して大気開
放手段（36）である三方弁（36a）の一つの開口に連結
されている。この三方弁は第２図で、その構成を後述す
るも三方弁の三つの開口中の二つ目の開口は大気に曝さ
れる様に成され、三つ目の開口はチューブ（8a）を介し
て圧力ゲージ（13）に連なり、更に第５図で説明した切
換弁（12）を介して吸引セル（１）連結されている。切
換弁（12）は希釈液槽（15）からの希釈液（16）を希
釈、撹拌部（18a）に送液すると共に、吸引セル（１）
で定量採取した組織液（25）を希釈、撹拌部（18a）に
送液する。皮膚（11）の角質層（11a）はストリッピン
グ処理で剥離され、表皮（11b）上に吸引セル（１）を
載置し、吸引して組織液（25）の採取が行なわれ、組織
液は真皮（11c）近傍から浸出される。Hereinafter, the tissue fluid suction and collection device of the present invention will be described with reference to FIGS.
The figure will be described in detail. FIG. 1 is an equivalent view of a main part of a tissue fluid suction / collection apparatus according to the present invention, and portions corresponding to those in FIG. 4 are denoted by the same reference numerals. In the figure, a suction pump (14a) constituting a suction means (14) is driven by a motor (14b) to reduce the pressure in a tube (8a). The suction port of the suction pump (14a) is connected via a tube (8a) to one opening of a three-way valve (36a), which is an atmosphere opening means (36). This three-way valve is shown in FIG. 2 and its configuration will be described later. The second of the three openings of the three-way valve is made to be exposed to the atmosphere, and the third opening connects the tube (8a). The pressure gauge (13) is connected to the suction cell (1) via the switching valve (12) described with reference to FIG. The switching valve (12) dilutes the diluent (16) from the diluent tank (15) and sends it to the stirring unit (18a), and the suction cell (1)
The tissue fluid (25) quantitatively collected in (1) is diluted and sent to the stirring section (18a). The stratum corneum (11a) of the skin (11) is peeled off by stripping, the suction cell (1) is placed on the epidermis (11b), and the tissue fluid (25) is collected by suction, and the tissue fluid is dermis (11c) Leach from the vicinity.
上述の大気開放手段（36）である三方弁（36a）を第
２図A,B,Cで説明する。第２図Ａは三方弁（36a）の側断
面図、第２図B,Cは三方弁（36a）の動作説明図である。
第２図A,B,Cで第５図との対応部分には同一符号にサフ
ィックスａを付して重複説明を省力するも弁本体（30
a）は有底円筒状と成され、その円周方向に90゜間隔で
開口（38A）（38B）（38C）が穿たれ、開口（38A）（38
B）は圧力ゲージ（13）と吸引セル（１）に連なるチュ
ーブ（8a）と結合され、開口（38C）はチューブ（8a）
と結合され、チューブ（8a）の開放端は大気（39）と接
している。有底円筒状の弁本体（30a）の内部にはくの
字状の空路（34a）が形成された弁棒（32a）が回動自在
に配設され、弁棒（32a）には軸（40）が嵌着され、弁
本体（30a）の有底円筒の開口部側にはフランジ（41）
を有する軸受部（42）が嵌着され、弁棒（32a）の軸（4
0）は軸受部（42）の中心孔（43）を介して突出され、
軸（40）の突出端にはハンドル（44）と一体化されたカ
ラー（45）が固定され、このカラー（45）にはボールと
スプリングから成るクリック部材を有し、フランジに90
゜間隔に穿ったノッチ（46）に係合する様に成されてい
る。The three-way valve (36a), which is the above-described air release means (36), will be described with reference to FIGS. FIG. 2A is a side sectional view of the three-way valve (36a), and FIGS. 2B and 2C are explanatory diagrams of the operation of the three-way valve (36a).
In FIGS. 2A, 2B, and 2C, parts corresponding to those in FIG.
a) is a cylindrical shape with a bottom, and the openings (38A), (38B), and (38C) are drilled at 90 ° intervals in the circumferential direction, and the openings (38A) and (38)
B) is connected to the tube (8a) connected to the pressure gauge (13) and the suction cell (1), and the opening (38C) is connected to the tube (8a)
And the open end of the tube (8a) is in contact with the atmosphere (39). A valve stem (32a) having a U-shaped air passage (34a) formed therein is rotatably disposed inside a bottomed cylindrical valve body (30a), and a shaft ( 40) is fitted, and a flange (41) is provided on the opening side of the bottomed cylinder of the valve body (30a).
The bearing (42) having a shaft is fitted to the shaft (4) of the valve stem (32a).
0) protrudes through the center hole (43) of the bearing (42),
A collar (45) integrated with the handle (44) is fixed to the projecting end of the shaft (40). The collar (45) has a click member consisting of a ball and a spring.
゜ It is designed to engage with notches (46) drilled at intervals.
上述の如き大気開放手段（36）の動作を説明する。先
ず組織液（25）を皮膚（11）から採取中（第５図Ａ参
照）は大気開放手段（36）は第２図Ｂの如き状態になる
様にハンドル（44）で回転スプール（31）を回転させ
て、弁棒（32a）の空路（34a）を開口（38A）と（38B）
に接する様にして吸引手段（14）の吸引ポンプ（14a）
を駆動して排気し、減圧する。次に切換弁（12）の液路
（34）内に組織液（25）が充満された状態で大気開放手
段（36）を形成する三方弁の回転スプール（31a）のハ
ンドル（44）を回転させて弁棒（32a）のくの字状の空
路（34a）を第２図Ｃに示す様に切換えると三方弁の開
口（38a）は大気に曝され、常圧の760mmHgの圧力が空路
（34a）→開口（38B）→チューブ（8a）→圧力ゲージ
（13）→チューブ（8a）→切換弁（12）の開口（33U）
→液路（34）に達して、液路（34）内の気泡（35）は押
しつぶされ、小さくなる。その結果、液路（34）内の組
織液（25）に対する気泡（35）の割合は減少し、正確な
定量の組織液が液路（34）内に充満されて、定量測定が
行なわれることになる。The operation of the air release means (36) as described above will be described. First, while collecting the tissue fluid (25) from the skin (11) (see FIG. 5A), the air release means (36) turns the rotating spool (31) with the handle (44) so as to be in the state as shown in FIG. 2B. Rotate to open the airway (34a) of the valve stem (32a) (38A) and (38B)
Suction pump (14a) of suction means (14) in contact with
Is driven to exhaust and reduce the pressure. Next, the handle (44) of the rotary spool (31a) of the three-way valve forming the atmosphere opening means (36) is rotated while the tissue fluid (25) is filled in the fluid path (34) of the switching valve (12). When the U-shaped air path (34a) of the valve stem (32a) is switched as shown in FIG. 2C, the opening (38a) of the three-way valve is exposed to the atmosphere, and a pressure of 760 mmHg of normal pressure is applied to the air path (34a). ) → Opening (38B) → Tube (8a) → Pressure gauge (13) → Tube (8a) → Opening of switching valve (12) (33U)
→ Reaching the liquid path (34), the bubbles (35) in the liquid path (34) are crushed and reduced. As a result, the ratio of the air bubbles (35) to the tissue fluid (25) in the fluid path (34) decreases, and an accurate quantitative tissue fluid is filled in the fluid path (34), and quantitative measurement is performed. .
上述の実施例では定量採取した組織液（25）を大気の
常圧中に曝して気泡を縮小させたが、第３図の腰部等価
図に示す様に吸引手段（14）は二重吸引ポンプ（14a）
（14c）で構成し、この吸引ポンプと切換弁（47）で構
成された増圧手段（37）を介して圧力ゲージ（13）及び
切換弁（12）の液路（34）に負圧及び常圧より圧力の高
い正圧を加える様になす。即ち、皮膚（11）から組織液
（25）を吸引する場合には弁（47a）を介して切換弁（1
2）の液路（34）に負圧を加え、切換弁（47b）は閉じて
置く、字に液路（34）に所定量の組織液（25）が採取さ
れたら切換弁（47a）を閉じ切換弁（47a）を開いて、正
圧をポンプ（14c）から与える様にして気泡（35）を大
気に曝するより小さくする様に成したものである。In the above-described embodiment, the tissue fluid (25) collected quantitatively was exposed to the atmospheric normal pressure to reduce bubbles, but as shown in the waist equivalent diagram of FIG. 3, the suction means (14) is a double suction pump ( 14a)
The pressure gauge (13) and the liquid passage (34) of the switching valve (12) are supplied with a negative pressure and pressure through the pressure increasing means (37) composed of the suction pump and the switching valve (47). A positive pressure higher than the normal pressure is applied. That is, when sucking the tissue fluid (25) from the skin (11), the switching valve (1) is passed through the valve (47a).
2) Apply a negative pressure to the fluid path (34) and close the switching valve (47b). When a predetermined amount of tissue fluid (25) is collected in the fluid path (34), close the switching valve (47a). The switching valve (47a) is opened to apply a positive pressure from the pump (14c) so that the bubbles (35) are made smaller than when the bubbles (35) are exposed to the atmosphere.
本発明は上述の様に構成したので大きな気泡が液路に
残留しないので正確な定量採取を行うことが出来て正確
に血糖値を測定出来る組織液吸引採取装置が得られる。Since the present invention is configured as described above, since a large bubble does not remain in the fluid channel, it is possible to perform accurate quantitative sampling and obtain a tissue fluid suction and sampling device capable of accurately measuring a blood glucose level.
本発明の組織液吸引採取装置によれば、気泡の影響を
受けない組織液を定量採取出来て正確に血糖値測定を行
うことが出来る。ADVANTAGE OF THE INVENTION According to the tissue fluid suction-sampling apparatus of this invention, the tissue fluid which is not influenced by a bubble can be sampled quantitatively, and a blood glucose level can be measured accurately.
第１図は本発明の組織液吸引採取装置の一実施例を示す
要部等価図、第２図は本発明に用いる大気開放手段の構
成図、第３図は本発明の組織液吸引採取装置の他の実施
例を示す要部等価図、第４図は組織液吸引採取装置の構
成図、第５図は従来の切換弁の構成図である。 （11）は皮膚、（12）は切換弁、（14）は吸引ポンプ、
（25）は組織液、（32）は弁棒、（34）は液路、（35）
は気泡、（36）は大気開放手段、（37）は増圧手段であ
る。FIG. 1 is an equivalent view of an essential part showing an embodiment of a tissue fluid suction / collection apparatus according to the present invention, FIG. 2 is a block diagram of an atmosphere opening means used in the present invention, and FIG. FIG. 4 is a block diagram of a tissue fluid suction and collection device, and FIG. 5 is a block diagram of a conventional switching valve. (11) skin, (12) switching valve, (14) suction pump,
(25) is a tissue fluid, (32) is a valve stem, (34) is a fluid channel, (35)
Is a bubble, (36) is an atmosphere opening means, and (37) is a pressure increasing means.
───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.6，ＤＢ名) A61B 5/14 300 A61B 5/14 310 A61B 10/00 103 ──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int. Cl. 6 , DB name) A61B 5/14 300 A61B 5/14 310 A61B 10/00 103
Claims (2)
せ、該吸引セル内を減圧して組織液を採取する様にした
組織液吸引採取装置に於いて、 上記吸引セルを吸引手段を介して吸引し、組織液を定量
採取する定量採取手段と、 上記定量採取手段内の気圧を大気に開放する大気開放手
段とを具備して成ることを特徴とする組織液吸引採取装
置。In a tissue fluid suction / collection apparatus in which a suction cell is brought into close contact with the skin of a subject such as a human body and the inside of the suction cell is decompressed to collect a tissue fluid, the suction cell is connected to the skin via a suction means. A tissue fluid suction / collection apparatus comprising: a constant volume sampling means for aspirating and quantitatively collecting a tissue fluid; and an atmosphere opening means for releasing the atmospheric pressure in the fixed volume sampling means to the atmosphere.
せ、該吸引セル内を減圧して組織液を採取する様にした
組織液吸引採取装置に於いて、 上記吸引セルを吸引手段を介して吸引し、組織液を定量
採取する定量採取手段と、 上記定量採取手段内の気圧を増圧する増圧手段とを具備
して成ることを特徴とする組織液吸引採取装置。2. A tissue fluid suction / collection apparatus in which a suction cell is brought into close contact with the skin of a subject such as a human body and the pressure inside the suction cell is reduced to collect a tissue fluid. A tissue fluid aspirating and sampling apparatus, comprising: a quantitative sampling means for aspirating the tissue fluid to quantitatively collect the tissue fluid; and a pressure increasing means for increasing the atmospheric pressure in the quantitative sampling means.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2259573A JP2958367B2 (en) | 1990-09-28 | 1990-09-28 | Tissue fluid suction sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2259573A JP2958367B2 (en) | 1990-09-28 | 1990-09-28 | Tissue fluid suction sampling device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04138136A JPH04138136A (en) | 1992-05-12 |
| JP2958367B2 true JP2958367B2 (en) | 1999-10-06 |
Family
ID=17336002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2259573A Expired - Lifetime JP2958367B2 (en) | 1990-09-28 | 1990-09-28 | Tissue fluid suction sampling device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2958367B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2684871B2 (en) * | 1991-05-16 | 1997-12-03 | 日本電気株式会社 | Body fluid component measuring method and measuring device |
| JPH0824680B2 (en) * | 1992-10-26 | 1996-03-13 | 日本電気株式会社 | Suction leachate sampling device |
| US12110972B2 (en) * | 2017-10-06 | 2024-10-08 | Nipro Corporation | Flow path switching device |
| CN114397141B (en) * | 2021-12-10 | 2022-11-29 | 连云港市东方医院 | Department of neurology is clinical with getting liquid device |
-
1990
- 1990-09-28 JP JP2259573A patent/JP2958367B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04138136A (en) | 1992-05-12 |
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