JP3014650B2 - Method for producing tungsten-containing chlorella - Google Patents
Method for producing tungsten-containing chlorellaInfo
- Publication number
- JP3014650B2 JP3014650B2 JP8359357A JP35935796A JP3014650B2 JP 3014650 B2 JP3014650 B2 JP 3014650B2 JP 8359357 A JP8359357 A JP 8359357A JP 35935796 A JP35935796 A JP 35935796A JP 3014650 B2 JP3014650 B2 JP 3014650B2
- Authority
- JP
- Japan
- Prior art keywords
- chlorella
- tungsten
- producing
- culture
- tungstate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000195649 Chlorella <Chlorellales> Species 0.000 title claims description 32
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 title claims description 26
- 229910052721 tungsten Inorganic materials 0.000 title claims description 26
- 239000010937 tungsten Substances 0.000 title claims description 26
- 238000004519 manufacturing process Methods 0.000 title claims description 10
- XMVONEAAOPAGAO-UHFFFAOYSA-N sodium tungstate Chemical compound [Na+].[Na+].[O-][W]([O-])(=O)=O XMVONEAAOPAGAO-UHFFFAOYSA-N 0.000 claims description 9
- AAQNGTNRWPXMPB-UHFFFAOYSA-N dipotassium;dioxido(dioxo)tungsten Chemical compound [K+].[K+].[O-][W]([O-])(=O)=O AAQNGTNRWPXMPB-UHFFFAOYSA-N 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 4
- 239000013505 freshwater Substances 0.000 claims description 4
- PBYZMCDFOULPGH-UHFFFAOYSA-N tungstate Chemical compound [O-][W]([O-])(=O)=O PBYZMCDFOULPGH-UHFFFAOYSA-N 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 2
- CMPGARWFYBADJI-UHFFFAOYSA-L tungstic acid Chemical compound O[W](O)(=O)=O CMPGARWFYBADJI-UHFFFAOYSA-L 0.000 claims description 2
- 230000029553 photosynthesis Effects 0.000 claims 1
- 238000010672 photosynthesis Methods 0.000 claims 1
- GKVKTECTPNRCFW-UHFFFAOYSA-N tungsten hydrochloride Chemical compound Cl.[W] GKVKTECTPNRCFW-UHFFFAOYSA-N 0.000 claims 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
- 241000195652 Auxenochlorella pyrenoidosa Species 0.000 description 6
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 240000009108 Chlorella vulgaris Species 0.000 description 2
- 235000007089 Chlorella vulgaris Nutrition 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910004803 Na2 WO4.2H2 O Inorganic materials 0.000 description 1
- 241001535061 Selenastrum Species 0.000 description 1
- -1 acetic acid Chemical class 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000001637 plasma atomic emission spectroscopy Methods 0.000 description 1
- CGGMOWIEIMVEMW-UHFFFAOYSA-N potassium tungsten Chemical compound [K].[W] CGGMOWIEIMVEMW-UHFFFAOYSA-N 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005245 sintering Methods 0.000 description 1
- PFUVRDFDKPNGAV-UHFFFAOYSA-N sodium peroxide Chemical compound [Na+].[Na+].[O-][O-] PFUVRDFDKPNGAV-UHFFFAOYSA-N 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 150000003657 tungsten Chemical class 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、タングステン成分が含
まれるクロレラの製造方法に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing chlorella containing a tungsten component.
【０００２】[0002]
【従来の技術】クロレラは、健康食品や飼料に市販され
ている。2. Description of the Related Art Chlorella is commercially available for health foods and feeds.
【０００３】クロレラには、蛋白質、脂肪、炭水化物、
ビタミンの他、各種のミネラルが約７％含まれており、
これ等のミネラルのうち、特にカルシウム、鉄が貴重な
供給源として注目されている。[0003] Chlorella contains proteins, fats, carbohydrates,
In addition to vitamins, it contains about 7% of various minerals,
Of these minerals, calcium and iron are particularly attracting attention as valuable sources.
【０００４】一方、最近注目されている抗酸化作用につ
いて、以下の論文にタングステンの効果が記述されてい
る（タングステン酸ソーダ療法：（財）健康生活促進協
会河野裕行著）。しかし、タングステンを単独で食用さ
れた例はなく、又、タングステンをほかの食品に混合し
て販売することは禁じられている。On the other hand, regarding the antioxidant action that has recently attracted attention, the effect of tungsten is described in the following paper (sodium tungstate therapy: written by Hiroyuki Kono, Healthy Living Promotion Association). However, there is no example of eating tungsten alone, and it is forbidden to mix and sell tungsten with other foods.
【０００５】又、海水中には、タングステンが微量含ま
れているが、海水中で増殖するマリンクロレラに、タン
グステンが含まれているということは知られていない。
更に、マリンクロレラは増殖力が弱く、一般には淡水型
のクロレラ・ピレノイドーサ等が商業生産に利用されて
いる。[0005] Further, although a very small amount of tungsten is contained in seawater, it is not known that marine chlorella growing in seawater contains tungsten.
Further, marine chlorella has a weak proliferation ability, and generally, freshwater chlorella pyrenoidosa and the like are used for commercial production.
【発明が解決しようとする課題】本発明は、従来不可能
であったタングステンを含有するクロレラの製造方法を
提供しようとするものである。SUMMARY OF THE INVENTION An object of the present invention is to provide a method for producing tungsten-containing chlorella, which has heretofore been impossible.
【０００６】[0006]
【課題を解決するための手段】本発明では、タングステ
ンを含む培養液中で、請求の範囲記載の条件下において
淡水クロレラ・ピレノイドーサを用いて、培養するよう
にしたものである。According to the present invention, the cultivation is carried out in a culture solution containing tungsten using freshwater chlorella pyrenoidosa under the conditions described in the claims.
【０００７】検討の結果、タングステンの濃度が５ｇ／
リットル（Ｗとして重量換算）以上の培養液中では、ク
ロレラが増殖するに至らない。又、タングステン酸カリ
はタングステン酸ソーダよりも培養しやすく、タングス
テンの含有量も多い。タングステン酸カリの濃度は、
０．０１〜４ｇ／リットルであり、特に、０．５〜３ｇ
／リットルの範囲の培養液を使用することが好ましいこ
とを発見した。タングステン酸ソーダの濃度は０．０１
〜３ｇ／リットルであり、特に０．５〜１．５ｇ／リッ
トルの範囲の培養液を使用することが好ましいことを発
見した。As a result of the study, the tungsten concentration was 5 g /
Chlorella does not grow in cultures of liters (weight conversion as W) or more. Potassium tungstate is easier to culture than sodium tungstate and has a higher tungsten content. The concentration of potassium tungstate is
0.01 to 4 g / liter, especially 0.5 to 3 g
It has been found that it is preferable to use a culture in the range of 1 / liter. The concentration of sodium tungstate is 0.01
It has been found that it is preferable to use a culture solution in the range of 0.5 to 1.5 g / l, especially 0.5 to 1.5 g / l.
【０００８】また、培養液に、タングステンを添加する
目的のためのタングステン酸イオン源としては、タング
ステン酸カリ、タングステン酸ソーダの他、水溶性のタ
ングステン塩、例えば、これらの混合物等がある。更
に、タングステン酸の他、水に不溶性であっても、タン
グステン酸カルシウム（ＣａＷＯ４）等は塩化アンモニ
ウムで可溶化して、使用することができる。本発明で
は、通常のクロレラ培養に使われる培養液を用いること
ができる。炭素源としては、酢酸などの有機酸やその
塩、炭酸ガスを使用する。培養には、太陽光又は人工光
を照射し培養液を循環させる。培養温度は、１５〜４０
℃の範囲で、特に２５〜３５℃が適当であり、ｐＨ４〜
９以下に調整することが望ましく、特に５〜８．５が適
当である。培養可能な温度、ｐＨの範囲はクロレラの緑
色の濃さで判定することができる。範囲を超えると色が
急に薄くなり元気がなくなるので直ぐ分る。本発明に使
用される淡水クロレラは、クロレラ・ピレノイドーサ、
クロレラ・ブルガリス、クロレラ・エリプリイデアのほ
か、セネデスムス、クロミドモナス、セレナストルムそ
の他のクロレラでもよい。 培養終了後、遠心分離し水
洗を数回繰り返して、クロレラ表面の付着物を取り去
り、次に、スプレードライ法、乾燥凍結法などにより乾
燥して、粉末状のクロレラが得られる。[0008] Tungstate ion sources for adding tungsten to the culture solution include potassium tungstate and sodium tungstate as well as water-soluble tungsten salts, for example, mixtures thereof. Furthermore, in addition to tungstic acid, even if it is insoluble in water, calcium tungstate (CaWO 4 ) or the like can be used after being solubilized with ammonium chloride. In the present invention, a culture solution used for ordinary chlorella culture can be used. As a carbon source, an organic acid such as acetic acid, a salt thereof, and carbon dioxide gas are used. For culture, the culture solution is circulated by irradiating sunlight or artificial light. The culture temperature is 15 to 40
In the range of 25 ° C., especially 25 to 35 ° C. is suitable, and pH 4 to
It is desirable to adjust the value to 9 or less, and particularly, 5 to 8.5 is appropriate. The cultivable temperature and pH range can be determined based on the green density of Chlorella. Beyond the range, the color suddenly fades and you lose your energy. The freshwater chlorella used in the present invention is Chlorella pyrenoidosa,
Chlorella vulgaris, Chlorella elipiriidea, Senedesmus, Clomidmonas, Selenastrum, and other chlorella may be used. After completion of the culture, centrifugation and washing with water are repeated several times to remove deposits on the chlorella surface, and then dried by a spray-drying method, a dry-freezing method or the like to obtain a powdery chlorella.
【０００９】[0009]
【実施例】表１、２に示した組成の培養液を使用してク
ロレラ・ピレノイドーサの培養を行った。Ｃ−１は、対
照のためのものであり、Ｃ−２は、一般に使用されてい
るタングステン酸ソーダを使用して、その添加量を検討
したものである。EXAMPLES Culture of Chlorella pyrenoidosa was performed using culture solutions having the compositions shown in Tables 1 and 2. C-1 is for a control, and C-2 is the amount of addition using sodium tungstate commonly used.
【表１】 [Table 1]
【表２】 ５リットルの三角コルベンに３リットルの培養液を入れ
て、クロレラ・ピレノイドーサ種株、約３００ｃｃを加
え、２５〜３５℃、直射日光下でエアレーションを行い
ながら培養した。ｐＨは、酢酸で調整し、ｐＨ５〜７．
５とした。培養開始直後、タングステン酸ソーダ（Ｎａ
２ＷＯ４・２Ｈ２Ｏ）及びタングステン酸カリ（Ｋ２Ｗ
Ｏ４）の所定濃度液をそれぞれの培養液中に加えて、２
１日間培養し、ＰＣＶ（Ｐａｃｋｅｄ Ｃｅｌｌ Ｖｏ
ｌｕｍｅ；クロレラ増殖量（ｍｌ／ｌ））が、夫々、約
２０前後に達した．[Table 2] 3 liters of the culture solution was added to a 5 liter triangular corben, and about 300 cc of Chlorella pyrenoidosa seed strain was added thereto, followed by culturing at 25 to 35 ° C under aeration under direct sunlight. pH is adjusted with acetic acid, pH 5-7.
It was set to 5. Immediately after the start of the culture, sodium tungstate (Na
2 WO 4 · 2H 2 O) and tungsten potassium (K 2 W
O 4 ) is added to each culture solution at a predetermined concentration, and 2
After culturing for one day, PCV (Packed Cell Vo
lume; Chlorella growth (ml / l)) reached about 20 respectively.
【００１０】なお、タングステンの添加量は、それぞれ
０．０１〜５ｇ／リットルで培養した。予想に反して高
濃度で培養が可能であり、５ｇ／リットル（Ｋ２Ｗ
Ｏ４）（Ｎａ２ＷＯ４・２Ｈ２Ｏ）ではともに殆ど成長
しない。その結果は図１のとおりである。The culture was carried out at a tungsten addition amount of 0.01 to 5 g / liter. Unexpectedly, cultivation at a high concentration is possible and 5 g / l (K 2 W
O 4 ) (Na 2 WO 4 .2H 2 O) hardly grows. The result is as shown in FIG.
【００１１】次に表３、４に示した培養液Ｂを使用して
クロレラ・ピレノイドーサの培養を行った。Next, using the culture solution B shown in Tables 3 and 4, chlorella pyrenoidosa was cultured.
【表３】 [Table 3]
【表４】 即ち、１５０リットルの培養槽に、１００リットルの培
養液を入れて、クロレラ・ブルガリス種株約１０リット
ルを加え、２５〜３５℃、直射日光下でエアレーション
を行いながら培養した。ｐＨは、酢酸で調整し、ｐＨ５
〜７．５とした。培養開始直後、Ｎａ２ＷＯ４・２Ｈ２
ＯおよびＫ２ＷＯ４を１〜３ｇ／リットル（タングステ
ン重量換算）をそれぞれの培養液中に加え、Ｃ−６は特
に１週間後更に１回（合計２回）添加し、２１日間培養
し、ＰＣＶ２０前後に達した。ＰＣＶは、タングステン
濃度１ｇ／ｌ（Ｎａ２ＷＯ４・２Ｈ２Ｏ）では、対照の
１０６％、３ｇ／ｌ（Ｎａ２ＷＯ４・２Ｈ２Ｏ）では、
対照の４４％、１ｇ／ｌ（Ｋ２ＷＯ４）では、対照の１
１７％、３ｇ／ｌ（Ｋ２ＷＯ４）では、対照の１０３％
である。[Table 4] That is, 100 liters of a culture solution was placed in a 150 liter culture tank, and about 10 liters of Chlorella vulgaris strain was added, followed by culturing at 25 to 35 ° C under direct sunlight under aeration. pH is adjusted with acetic acid, pH 5
To 7.5. Immediately after the start of culture, Na 2 WO 4 · 2H 2
O and K 2 WO 4 are added at 1 to 3 g / liter (in terms of tungsten weight) to the respective culture solutions, and C-6 is further added once more, especially after one week (two times in total), and cultured for 21 days. It reached around PCV20. PCV is the concentration of tungsten 1g / l (Na 2 WO 4 · 2H 2 O), 106% of the control, the 3g / l (Na 2 WO 4 · 2H 2 O),
At 44% of the control, 1 g / l (K 2 WO 4 )
At 17%, 3 g / l (K 2 WO 4 ), 103% of the control
It is.
【００１２】これを遠心分離し、水洗を繰り返して十分
にクロレラに付着しているものを取り除き、凍結乾燥し
た。この乾燥クロレラ中に含まれるタングステンをＩＣ
Ｐ法で分析した。This was centrifuged, washed repeatedly with water to remove the substance sufficiently adhered to Chlorella, and freeze-dried. Tungsten contained in this dried chlorella is used for IC
Analysis was performed by the P method.
【００１３】ここで使用した、ＩＰＣ（誘導プラズマ発
光分光分析）法は次のような方法である。試料をアルミ
ナるつぼにはかり取り、可焼炉で灰化する。灰化後、過
酸化ナトリウムを加えて加熱融解する。放冷後、融解生
成物をビーカーにとり、リン酸及び塩酸を加え溶解した
後、加熱する。常温まで冷却した後、フラスコに水を加
えて全量を移し入れ、更に、水で標線まで薄める。この
溶液の一部を、ＩＣＰ装置のアルゴンプラズマ中に噴霧
して、その発光強度を測定する。The IPC (Induction Plasma Emission Spectroscopy) method used here is as follows. The sample is weighed in an alumina crucible and incinerated in a sintering furnace. After incineration, add sodium peroxide and heat to melt. After cooling, the molten product is placed in a beaker, phosphoric acid and hydrochloric acid are added and dissolved, and then the mixture is heated. After cooling to room temperature, water is added to the flask to transfer the whole amount, and further diluted with water to the marked line. A part of this solution is sprayed into an argon plasma of an ICP device, and the emission intensity is measured.
【００１４】この結果は表５の通り、それぞれ高濃度の
タングステンを含むクロレラを得た。特に、Ｃ−６は、
３．１％の高濃度のタングステンを含むクロレラが得ら
れた。The results are shown in Table 5. Chlorella containing tungsten at a high concentration was obtained. In particular, C-6 is
Chlorella with a high concentration of tungsten of 3.1% was obtained.
【表５】 [Table 5]
【００１５】[0015]
【発明の効果】本発明は以上のように構成されているの
で、タングステンを含有するクロレラを製造することが
できるという優れた効果を有する。As described above, the present invention has an excellent effect that a chlorella containing tungsten can be manufactured.
【図１】タングステン添加量とクロレラの増殖量（ＰＣ
Ｖ）の関係を示すグラフ（資料Ｎｏ．Ｃ−２）。増殖可
能なタングステン酸ソーダの濃度は０．０１〜３ｇ／ｌ
で、最適な濃度は０．５〜１．５ｇ／ｌであることを示
している。Fig. 1 Tungsten addition and Chlorella growth (PC
A graph showing the relationship V) (Document No. C-2). The concentration of sodium tungstate capable of growing is 0.01 to 3 g / l.
Indicates that the optimum concentration is 0.5 to 1.5 g / l.
【図２】同（資料Ｎｏ．Ｃ−２Ｂ）。増殖可能なタング
ステン酸カリの濃度は０．０１〜４ｇ／ｌであり、最適
な濃度は０．５〜３ｇ／ｌであることを示している。FIG. 2 (Document No. C-2B). The concentration of potassium tungstate capable of growing is between 0.01 and 4 g / l, indicating that the optimal concentration is between 0.5 and 3 g / l.
【図３】クロレラの増殖量（ＰＣＶ）の経時変化を示す
グラフ（資料Ｎｏ．Ｃ−１，Ｃ−３，Ｃ−６）FIG. 3 is a graph showing the time-dependent change in the growth amount (PCV) of Chlorella (Document Nos. C-1, C-3, and C-6).
Claims (6)
中でpH４〜９，培養温度１５〜４０℃の条件下で光合成
によってクロレラを培養することを特徴とするタングス
テン含有クロレラの製造方法。1. A method for producing tungsten-containing chlorella, comprising culturing chlorella by photosynthesis in a culture solution containing tungstic acid or a salt thereof at a pH of 4 to 9 and a culture temperature of 15 to 40 ° C.
カリを用いた請求項１記載のタングステン含有クロレラ
の製造方法。2. The method for producing a tungsten-containing chlorella according to claim 1, wherein potassium tungstate is used as the tungstate.
ソーダを用いた請求項１記載のタングステン含有クロレ
ラの製造方法。3. The method for producing a tungsten-containing chlorella according to claim 1, wherein sodium tungstate is used as the tungsten hydrochloride.
求項１，２又は３記載のタングステン含有クロレラの製
造方法。4. The method for producing a tungsten-containing chlorella according to claim 1, wherein fresh water chlorella is used as the chlorella.
４ｇ／リットルとした請求項２又は４記載のタングステ
ン含有クロレラの製造方法。5. The concentration of potassium tungstate of 0.01 to 0.01
The method for producing a tungsten-containing chlorella according to claim 2 or 4, wherein the amount is 4 g / liter .
〜３ｇ／リットルとした請求項３又は４の記載のタング
ステン含有クロレラの製造方法。6. A sodium tungstate concentration of 0.01.
The method for producing a tungsten-containing chlorella according to claim 3 or 4, wherein the amount is -3 g / liter .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8359357A JP3014650B2 (en) | 1996-12-12 | 1996-12-12 | Method for producing tungsten-containing chlorella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8359357A JP3014650B2 (en) | 1996-12-12 | 1996-12-12 | Method for producing tungsten-containing chlorella |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH10165171A JPH10165171A (en) | 1998-06-23 |
| JP3014650B2 true JP3014650B2 (en) | 2000-02-28 |
Family
ID=18464097
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8359357A Expired - Fee Related JP3014650B2 (en) | 1996-12-12 | 1996-12-12 | Method for producing tungsten-containing chlorella |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3014650B2 (en) |
-
1996
- 1996-12-12 JP JP8359357A patent/JP3014650B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH10165171A (en) | 1998-06-23 |
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