JP3029986B2 - Alcohol decomposable composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an alcohol-decomposable composition containing an acetic acid bacterium. It relates to an alcohol-decomposable composition which makes it possible to prevent absorption. In this specification, the term "alcohol"
Unless otherwise specified, it means ethyl alcohol.

[0002]

2. Description of the Related Art Alcoholic beverages are delicious, have excellent properties such as relieving work tension and lightening the mood, and are widely consumed in family gatherings, social gatherings, banquets and other social occasions. However, symptoms such as drunkenness, sickness and hangovers appear with an increase in the alcohol concentration in the blood, for example, when alcohol is excessively consumed or when a person weak in alcohol is socially drunk. Various drugs have been developed to alleviate such symptoms.
There are alcohol absorption inhibitors disclosed in 3-127739, alcohol metabolism promoters disclosed in JP-A-4-124133, and the like, and further JP-A-49-132286 discloses stomach and the like. An enzyme solution of yeast that degrades alcohol in the digestive tract is disclosed. In particular, although this enzyme solution directly decomposes alcohol, alcohol dehydrogenase and aldehyde dehydrogenase contained in this enzyme solution exhibit activity only in an alkaline region of pH 7 or higher,
It has no effect in the stomach, which secretes acidic digestive juices, and is almost inactivated during its transfer from the stomach to the intestine. For this reason, the enzyme solution of yeast has a problem in its effectiveness.

[0003]

DISCLOSURE OF THE INVENTION The present invention provides an alcohol-decomposable composition which is administered before or before ingesting an alcoholic beverage, thereby decomposing alcohol in the stomach and preventing excessive alcohol absorption. The purpose is to do.

[0004]

Means for Solving the Problems Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) possessed by ordinary yeast have an activity on the alkaline side at a pH of about 7.5 to 10.0. Inside the stomach, alcohol cannot be broken down. In addition, the alcohol-metabolizing enzyme system including membrane-bound ADH and ALDH of acetic acid bacteria has an optimum pH of around 4, and has almost no activity at pH 3 or lower, so that alcohol cannot be sufficiently decomposed in the stomach. Was thought. In contrast, the present inventors have discovered that the cell suspension itself before extracting membrane-bound ADH and ALDH of acetic acid bacteria shows an enzyme activity of oxidizing alcohol even at pH 3 or less. If a acetic acid bacterium having a characteristic feature is taken before or before drinking alcohol, it was found that the acetic acid bacterium oxidizes alcohol in the stomach to acetic acid via acetaldehyde, and the alcohol is not absorbed in the digestive tract. The present invention is an invention made based on such knowledge, and therefore, the present invention
It is an object of the present invention to provide an alcohol-decomposable composition containing an acetic acid bacterium that decomposes alcohol in an acidic region of H3 or less. Hereinafter, the contents of the present invention will be described in detail.

In the present invention, an acetic acid bacterium can be used without any particular limitation as long as it decomposes alcohol at a pH of 3 or less and is harmless to the human body. Preferred acetic acid bacteria that can be used in the present invention are as follows. The IFO number is a reference number given to the deposited bacteria of the Fermentation Research Institute. (Acetobacter aceti) Acetobacter aceti IFO 3281, IFO 3283, IFO 3284 Acetobacter acetigenus IFO 3279 Acetobacter acetosus IFO 3296 Acetobacter ascendens IFO 3296 Acetobacter aurantius IFO 3245, IFO 3247, IFO 3248 Acetobacter kutzingianus IFO 3222 Acetobacter rances AFO 3297, IFO 3298 Acetobacter xylinus IFO 3288 Acetobacter pasteurianus IFO 3223 Acetobacter turbidans IFO 3225

(Gluconobacter genus) Gluconobacter melanogenus IFO 3294 Gluconobacter oxydans IFO 3287 Gluconobacter dioxyacetonicus IFO 3271, IFO 3272, IFO 3274 gluco Gluconobacter cerinus IFO 3262, IFO 3263, IFO 3264 IFO 3265, IFO 3266, IFO 3267 IFO 3268, IFO 3269, IFO 3270 Gluconobacter gluconicus IFO 3285, IFO 3286 Gluconobacter luconobacter G albidus) IFO 3251, IFO 3253 Gluconobacter suboxydans IFO 3290, IFO 3291, IFO 12528

In the present invention, either acetic acid bacteria that can grow (hereinafter referred to as active acetic acid bacteria) or acetic acid bacteria that cannot grow (hereinafter referred to as inactive acetic acid bacteria) may be used. When using active acetic acid bacteria, after culturing the acetic acid bacteria, centrifugation is used to separate the medium into acetic acid bacteria and collect the cells. Or a method in which the collected bacterial cells are freeze-dried to powder.
Examples of a method for preparing an inactive acetic acid bacterium include a method of heat-treating the acetic acid bacterium at 40 to 60 ° C. for 30 to 60 minutes, and a method of mechanically or ultrasonically crushing the acetic acid bacterium. With the alcohol-decomposable composition of the present invention, the intake amount for obtaining the effect of lowering the alcohol concentration in the stomach is very large depending on the weight, constitution, etc. of the person who takes it, and the difference between individuals and the amount of alcohol consumption are large. Although it cannot be clearly identified due to the effect, in order to sufficiently exert the effect of decomposing alcohol in the stomach, a single intake is in the range of 0.01 to 25 g, preferably 0.05 to 5 g. It is desirable.

[0008] The composition of the present invention may be in the form of powder, tablet,
It may be any of capsules, granules, liquids, beverages and the like. Also, depending on the form of the composition, fillers, excipients,
Add additives such as It can also be used as a beverage. As an example, 0.05 to 5 g of the present composition is added to 10% of sugar, 0.1% of sourness, 2 to 3% of fruit juice, 0.1% of flavor, and the pH is adjusted to 3.5 to 4.0. After that, it is preferable to sterilize by heating at 65 ° C. for 10 minutes. It is preferable to drink the obtained product after refrigeration.

[0009]

The alcohol-decomposable composition of the present invention has p
Alcohol and acetaldehyde are rapidly decomposed even with H3 or less, thereby preventing excessive alcohol absorption. Furthermore, the acetic acid bacterium of the present invention can be used for ordinary membrane-bound ADH and
Since it also contains LDH, it decomposes alcohol more strongly when the inside of the stomach becomes pH 3 or more. Therefore, the alcohol-decomposable composition of the present invention has a strong alcohol-decomposition action and prevents excessive absorption of alcohol.

[0010]

【Example】

(Example 1) The relationship between ADH activity and ALDH activity of acetic acid bacteria and pH was examined. Acetobacter aceti (IF
O 3284) was uniformly dispersed in 20 ml of distilled water and freeze-dried to obtain a cell powder. The activity of both enzymes is W
The method was carried out according to the method of using ferricyanide as an electron acceptor by Wood et al. The reduction ratio of ferricyanide to ferrocyanide corresponds to the amount of oxidized alcohol and the amount of acetaldehyde. Reaction composition is 10μ
mol potassium ferricyanide, 0.5 ml Mc of various pH
Ilvaine buffer, 100 μmol alcohol or 20 μmol acetaldehyde, and acetic acid bacteria powder
A bacterial solution containing 0.1 mg was added to make a total volume of 1.0 ml. The reaction was started by adding potassium ferricyanide and performed at 37 ° C. for 20 minutes. Immediately, 0.5 ml of Dupanol solution was added to stop the enzymatic reaction. Then, add 3.5 ml of distilled water, mix well, and leave at room temperature for 20 minutes.
The sisn blue color was measured at 660 nm. The activity was expressed as one unit when the activity of 1 μmol of alcohol or acetaldehyde was reduced in one minute. As a result of the experiment, FIG.
As shown in the figure, the optimal pH of ADH of acetic acid bacteria is around 4,
In the case of DH, it is around pH 5, but at pH 3, ADH and ALDH show little activity.

(Example 2) The relationship between the enzyme activity involved in the oxidation of alcohol by acetic acid bacteria and the pH was examined by conducting experiments. The experimental procedure was as follows. The activity of the enzyme system involved in the oxidation of alcohol was measured from the rate of oxygen consumption using a dissolved oxygen meter. That is, in a 10 ml beaker (2.5 × 5 cm), McIlvaine buffer 4.6 of various pH was added.
After adding 0.2 ml of the bacterial solution containing 1 mg of the acetic acid bacteria powder prepared in Example 1, 1.2 ml of 1.0 M alcohol was added, and the enzyme reaction was carried out at 25 ° C. The activity was expressed as one unit in which 1 μmol of oxygen was consumed per minute. As a result, the enzyme activity involved in the oxidation of alcohol exhibits an optimum pH around pH 4, but as shown in the graphs of Table 1 and FIG.
Assuming 0%, 33% at pH 2.5 and 68.8% at pH 3.0
Met. Example 1 As can be seen from Example 2, the enzymatic activity involved in the oxidation of alcohol has a stronger alcohol-decomposing activity in the low-acid region of pH 3 or lower than the ADH and ALDH activities of acetic acid bacteria. Therefore, it is found that the enzyme system involved in the oxidation of the alcohol contained in the acetic acid bacteria powder works sufficiently even in the stomach having a low pH.

[0012]

[Table 1] Table 1. Relationship between alcohol-decomposing activity and enzyme activity of acetic acid bacteria pH 1.5 2.0 2.5 3.0 4.0 Enzyme activity 10.8 21.5 495.1 1033.2 1501.4 (unit / ml) * 0.007% 0.014% 33.0% 68.8% 100.0% * Strength at each pH when enzyme activity at pH 4.0 is 100%

Example 3 Next, animal experiments show that the alcohol-decomposable composition of the present invention degrades alcohol in the stomach. Acetobacter aceti (IFO 3284) or
A suspension of 25 mg of freeze-dried cells of Gluconobacter Suboxydans (IFO 12528) in 2.0 ml of water and 2.0 ml of a 10% ethanol solution are collected in separate test tubes. Male Wistar rats (8 to 10 weeks old), which had been fasted for 24 hours, were anesthetized and laparotomized. After mixing each of the above solutions for several seconds, the whole amount was immediately administered to the stomach with a syringe. Thereafter, at 0, 5, 10, 15 minutes, about 150 μl of blood was introduced from a cannulation tube placed in the inferior vena cava.
One by one was collected. 0.33 ml in 100 μl of collected blood
Per liter of perchloric acid (800 μl), mix and add
After centrifugation at 00 rpm for 5 minutes, the amount of ethanol in the supernatant was measured using an ethanol measurement kit (Boehringer Mannheim). At the same time, as a control, 2.0 ml of distilled water and 2.0 ml of a 10% ethanol solution were administered to the stomach of a rat with a syringe, and the measurement was similarly performed. Figure 3 shows the measurement results.
Is shown in the graph. As is clear from FIG. 3, it was found that the rats administered the acetic acid bacterium cell suspension had a clearly lower blood alcohol concentration, and a significant difference was seen at a risk rate of 7%. Therefore, it is clear that the alcohol-decomposable composition of the present invention, when present in the stomach, degrades alcohol and suppresses excessive absorption of alcohol.

[Brief description of the drawings]

FIG. 1 is a graph showing the relationship between the activity of ADH and ALDH of acetic acid bacteria and pH.

FIG. 2 is a graph showing the relationship between the activity of an enzyme system involved in the oxidation of alcohol by acetic acid bacteria and pH.

FIG. 3 is a graph showing the blood alcohol concentration of rats to which an acetic acid bacteria cell suspension was administered.

Claims (2)

(57) [Claims] 1. A pharmaceutical composition for decomposing alcohol, comprising an inactive acetic acid bacterium which has been heat-treated at 40 to 60 ° C. for 30 to 60 minutes and decomposes alcohol at a pH of 3 or less. 2. A food containing an inert acetic acid bacterium which has been heat-treated at 40 to 60 ° C. for 30 to 60 minutes and which decomposes alcohol at a pH of 3 or less.