JP3040992B2 - Food composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a food product having a whitening function containing proanthocyanidin and glutathione, more preferably collagen or collagen hydrolyzate as an active ingredient.

[0002]

2. Description of the Related Art A whitening effect in skin beauty has been regarded as important, and many inventions have been made in the form of cosmetics, oral medicines, bath preparations and the like. Above all, many cosmetics having a whitening effect have been developed. In the case of cosmetics that are expected to have a whitening effect when applied to the skin, herbal extracts such as water-soluble vitamins and salts thereof, colloidal sulfur, glutathione, kojic acid, tannin, lemon balm and thyme have been used as functional materials. Things. These are intended to suppress the biosynthesis of melanin, which causes dullness of the skin, by antioxidant activity and inhibition of tyrosinase activity.Cosmetics prepared by using these materials alone or in combination with them can be directly applied to the skin. By applying the composition, a whitening effect is expected. For example, a whitening cosmetic product containing lemon balm and thyme extract alone or in combination (JP-A-6-1996)
47), a whitening cosmetic containing a proteoglycan extract and ascorbic acid phosphate (JP-A-4-21061)
4), a whitening cosmetic comprising proanthocyanidin as an active ingredient (JP-A-2-134309) and a whitening effect characterized by using cysteine, Clara extract and the like as other tyrosinase inhibitors in order to further enhance the effect of proanthocyanidin. With cosmetics (Japanese Unexamined Patent Publication No. 6-33641)
8) and so on.

[0003] Pharmaceuticals that remove skin stains include SH-based preparations such as cysteine and glutathione;
Whitening medicines containing colloidal sulfur or hydroquinone and whitening medicines containing tranexamic acid and ascorbic acid (JP-A-4-243825) can be mentioned.

As foods, many foods containing water-soluble vitamins such as kojic acid, grape seed extract and ascorbic acid, or salts thereof have been developed. For example, kojic acid and fatty acid esters of kojic acid are contained. Whitening foods (JP-A-60-137253).

Proanthocyanidins, which are widely used in whitening cosmetics for skin application in anticipation of tyrosinase inhibitory activity, are absorbed by the intestinal tract without being decomposed by stomach acids and digestive enzymes when taken orally, and taken up into the blood. Has been reported to be transferred to the skin where melanin pigments are produced due to the release (Laparra J., Michaud J., masque
lier J., Plantes medicin. Et Phytother. 11,133
(1977)), has attracted attention as a whitening functional material that can be taken orally.

[0006]

As described above, whitening is regarded as important from the viewpoint of beauty. However, in the case of cosmetics or pharmaceuticals to be applied to the skin, the effect of perspiration decreases due to the effect of sweating over time. As a result, there is a problem that the whitening effect is insufficient or that the effect is only locally applied to the portion applied to the skin. Some of the active ingredients are irritating to the skin and their use is restricted, or have an unpleasant odor, and those which cause precipitation and aggregation and lack stability. In addition, it has been pointed out that decomposition and denaturation due to external stimuli such as fluctuations in the pH of the skin surface due to light, heat, and sweat lower the effect. Therefore, in the case of products that are expected to be whitening, those that can be taken orally can solve the problems caused by these external influences and have desirable functions.

For these reasons, oral whitening foods and pharmaceuticals having a whitening effect have been developed. However, in these cases, there is a concern about the possibility of onset of allergy due to the active ingredient occurring due to oral ingestion, and unexpected side effects due to the influence on metal ions essential for the action of digestive enzymes and the like. Furthermore, stomach acid,
It has been pointed out that there are problems that can occur due to oral ingestion, such as a problem that a sufficient whitening effect cannot be expected due to decomposition and modification of the active ingredient by digestive enzymes, and furthermore, a decrease in the rate of digestion and absorption of the active ingredient from the intestinal tract.

[0008] That is, it has been pointed out that the products which are expected to provide a whitening solution that can be orally ingested and which have been developed to date do not have sufficient functions and safety.
The present invention solves these problems, and has a sufficient whitening effect,
It is an object of the present invention to provide a food composition having safety.

[0009]

In the present invention, attention has been paid to proanthocyanidins as one of the active ingredients having a whitening effect. Proanthocyanidins are polyphenols contained in grape seeds, avocados, strawberries and other fruits, and barley and other cereals defined as "compounds that produce anthocyanidins by cleavage of CC bonds". As described above, it has been confirmed that when taken orally, it is transferred to the skin without being decomposed, and because it is a natural product contained in foods with a long eating experience, its safety has been confirmed. . However, the whitening effect when taken orally is not at a sufficiently satisfactory level as described above.

Therefore, the present inventors have made intensive studies on improving the whitening effect of proanthocyanidin. As a result, it was found that the oral administration of proanthocyanidin and glutathione in combination can promote the skin whitening effect synergistically. all right. More preferably, it was found that oral intake of collagen or collagen hydrolyzate in combination also promoted the skin whitening effect extremely synergistically, and completed the food composition according to the present invention.

[0011] That is, the food composition of the present invention is characterized by containing both proanthocyanidin and glutathione, and more preferably, is characterized by containing collagen or collagen hydrolyzate.

[0012]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below. In the present invention, as proanthocyanidins, grapes, blueberries, strawberries, avocado and other fruits, crushed seeds, or barley, wheat, red beans, soybeans, crushed foods containing proanthocyanidins such as black soybeans or extract extract It is also possible to use such food ingredients.
The outline of a method for producing a grape seed extract as an example of a method for processing these food ingredients containing proanthocyanidins is as follows.

After drying the grape seeds at 40 ° C. for 24 hours under ventilation, the grape seeds are pulverized using a kneader, and 5 parts by weight of 70% aqueous alcohol is used for 1 part by weight of the pulverized grape seeds. Extract at 40 ° C. for 4 hours. The extract is produced by fractionating the solvent, purifying it with a dialysis membrane, and concentrating in vacuo. Another manufacturing method is disclosed in JP-A-63-16
2685, JP-A-63-267774, JP-A-03-2
00781 and the like. It is needless to say that the above-mentioned production method is merely an example, and the present invention is not limited thereto.

Glutathione used in the present invention is defined as a tripeptide composed of three amino acids, glutamic acid, cysteine and glycine. In the present invention, glutathione is preferably used as a glutathione-containing yeast cell powder, a yeast cell extract, or glutathione-containing cow, pig, or bird liver, or an extract thereof. The reason is that glutathione, as is well known, cannot be added as food or isolated glutathione has problems such as off-flavor and aggregation, but in the case of yeast cells for food containing glutathione, or extracts, there are no such problems. It depends.

As an example, a production example of a glutathione-containing yeast cell powder will be described. Baker's yeast (Saccharomyces cerevicia)
e) is repeatedly subcultured in a restriction medium, and a strain having a property containing a high content of glutathione is liquid-cultured.
After culturing, the cells are collected by filtration, dried at 24 ° C., and then powdered for production.

In the present invention, more preferably used collagen or collagen hydrolyzate is a protein or protein hydrolyzate obtained by extracting from an animal skin, bone and other connective tissues, or hydrolyzing the extract. It also includes gelatin which is a heat denaturing component of collagen.

An example of a production method using bovine skin will be described. First, beef skin is boiled down in hot water, tanned,
Collagen is obtained by obtaining a gel substance and purifying it by deodorization. Collagen degradation products are produced by further reducing the molecular weight of this collagen. As molecular weight reduction, alkali hydrolysis, acid hydrolysis, pressurized hydrolysis, enzymatic hydrolysis and the like can be used, and any of them can be used without any problem. For example, in the case of acid hydrolysis, it is produced by thermal decomposition in about 1N hydrochloric acid, neutralization, decolorization of activated carbon, desalting, and concentration.

The degraded collagen degraded in this manner is a total of amino acids and peptides separated from the collagen protein. In the present invention, the mixture or the difference in the molecular weight distribution does not matter under any conditions. Things can also be used. In the present invention, collagen or a suitable collagen hydrolyzate may be selected or used in combination depending on the suitability for processing as a food.

The food composition according to the present invention is characterized by containing proanthocyanidin and glutathione as active ingredients, and more preferably, collagen or collagen hydrolyzate as an active ingredient. In the present invention, the antioxidant activity of proanthocyanidins (Biol. Pharm. Bull., 17 (12), 1613-1515 (19
94)), tyrosinase inhibitory activity (FOOD style 21, 2 (1),
66-70 (1998)) and the antioxidant effect of glutathione (Int Cong.
Pestic Chem. 4th, 547 (1978)), detoxification (Adv. Enzymo
l., 46, 383 (1987)), a complementary whitening effect by sulfur-containing amino acids was found, which was so remarkable as to be incapable of being taken alone. Synergistic effects due to different antioxidant effects of proanthocyanidins and glutathione,
Alternatively, it is supposed that the effect of sulfur-containing amino acid of glutathione promotes the inhibition of tyrosinase of proanthocyanidin, and that the effect is maintained and the permeability to skin tissue is improved.

In the present invention, preferably, collagen,
Alternatively, a collagen decomposition product is added, in which case the whitening effect is further promoted. Oral ingestion of collagen or collagen degradation promotes skin metabolism (FOOD style 21, 2 (1), 54-60 (1998)), but this effect is due to the antioxidant effects of proanthocyanidins and glutathione. Was synergistically promoted, and furthermore, the detoxification effect of glutathione was presumed to have promoted the metabolism of the skin, but a previously unexpected whitening effect was observed.

The whitening effect in the present invention is caused synergistically by the action of metabolic levels as a result of the absorption of a small amount of the active ingredient into the body. Therefore, the combination of proanthocyanidin and glutathione, collagen, or collagen hydrolyzate is used. The ratio is not important, and may be any ratio, and it is important for the whitening effect of the present invention that proanthocyanidin and glutathione coexist, or that collagen or collagen degradation components coexist. That's it. As an example of the compounding standard, 10 to 2000 mg, preferably 50 to 200 mg, of proanthocyanidin per day of the whitening food composition is taken.
It may be blended in the whitening composition such that the amount of collagen or collagen hydrolyzate becomes 10 mg to 1000 mg, preferably 100 mg to 10 mg, and collagen or collagen hydrolyzate becomes 20 mg to 10 g, preferably 100 mg to 5 g. However, it is not limited to this combination.

In addition, water-soluble vitamins, fat-soluble vitamins,
It is perfectly acceptable to mix various minerals and the like at an arbitrary ratio, and this does not impair the function of the food composition of the present invention. Further, the food composition according to the present invention can be processed into any food in combination with general food processing raw materials. For example, it can be processed into any food such as tablets, capsules or beverages, confections, bread, etc., without impairing the function of the food composition according to the present invention.

[0023]

EXAMPLES A grape seed extract as a source of proanthocyanidins, a cell extract of brewer's yeast producing glutathione as a source of glutathione, and a collagen peptide obtained by extracting and hydrolyzing bovine dermis as a source of collagen are described below. The effect of using is described as an example. However, the present invention is not limited to these embodiments.

Example 1 The following composition was prepared. Composition 1 grape seed extract 10 parts by weight Glutathione beer yeast extract 0.15 parts by weight Composition 2 grape seed extract 10 parts by weight Glutathione beer yeast extract 0.15 parts by weight Collagen peptide 10 parts by weight Composition 3 grape seed extract 20.15 Parts by weight Composition 4 Glutathione beer yeast extract 20.15 parts by weight Composition 5 collagen peptide 20.15 parts by weight

Each of the compositions 1 to 5 was ingested at a rate of 10 g per day to 10 monitors for 4 weeks, and the whitening effect of the skin and the degree of increase in the skin lightness were determined by questionnaire. After the ingestion period, the subjects self-evaluated the degree of improvement of good makeup, spots and freckles, and dullness of the skin, and scored 5 points for significant improvement, 3 points for moderate improvement, and 1 point for mild improvement. The points and invariance were set to 0, and the average of 10 subjects was taken. As shown in Table 1, the results are shown in Table 1, as compared with Composition 3, Composition 4, and Composition 5. Composition 1 in which grape seed extract and glutathione brewer's yeast extract were used in combination, and composition 2 in which collagen peptide was further added were better. The whitening effect was clearly higher.

[0026]

[Table 1]

Before and after ingestion, the lightness (L value) of the skin was measured using a colorimeter (CR-200 (Minolta Tok)).
yo)), and the change (ΔL value) from that before ingestion was observed. (See FIG. 1). FIG. 1 shows that the ingestion of the composition 1 clearly increases the skin lightness, that is, the brightness of the skin, as compared to the other compositions 3, 4, and 4. Furthermore, the ingestion of the composition 2 shows that the skin lightness is further increased. As this result,
It was clear that the composition 1 and the composition 2 in the present invention had an extremely strong whitening effect as compared with the target composition. The monitor of this example did not show any allergic symptoms or side effects as pointed out in the conventional whitening products.

Example 2 Granules containing 5% by weight of the composition prepared in Example 1 were produced according to the following formulation. The granules were fed to 10 monitors for 2 weeks per day for 4 weeks to examine the skin whitening effect.

Granule A Composition 1 5% by weight Ascorbic acid 20% by weight Erythritol 50% by weight Excipients and flavors Granules B Composition 2 10% by weight Ascorbic acid 20% by weight Erythritol 50% by weight Excipients and flavors Granules C Composition 3 10% by weight Ascorbic acid 20% by weight Erythritol 50% by weight Excipients and flavors Granules D Composition 4 10% by weight Ascorbic acid 20% by weight Erythritol 50% by weight Excipients and flavors Granules E Composition 5 10% by weight Ascorbic acid 20% by weight Erythritol 50% by weight Excipients, flavors

After the ingestion period, the subjects self-evaluated the degree of improvement of the cosmetic paste, stains and freckles, and dullness of the skin, and scored 5 points of remarkable improvement and 3 points of moderate improvement.
10 points for each subject, 1 point for mild improvement and 0 point for unchanged
The average of the people was taken. Table 2 shows the results.

[0031]

[Table 2]

As can be seen from the results, it is clear that the granule A containing the composition 1 in the present invention has an extremely strong whitening effect as compared with the target granules C, D and E. Furthermore, it was clear that the granule B containing the composition 2 can obtain a higher whitening effect. Granule A,
On the monitor of the granule B, allergic symptoms and other side effects as pointed out in the conventional whitening products were not observed.

[0033]

According to the present invention, a food composition containing a combination of proanthocyanidin and glutathione, more preferably a proanthocyanidin, glutathione, collagen and a collagen hydrolyzate as active ingredients is effective as a whitening food.

[Brief description of the drawings]

FIG. 1 is a diagram showing the results of measuring the lightness of the skin before and after ingestion of the composition of the present invention and a comparative composition.

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁷ Identification code FI A61K 7/00 A61K 7/00 K X // A61K 31/35 602 31/35 602 35/78 35/78 C 38/00 37 / 02 (72) Inventor Kosuke Hayami 12-1 Kamishinano, Totsuka-ku, Yokohama-shi, Kanagawa Prefecture Inside FANCL Central Research Laboratory (72) Inventor Kenichi Ishiwatari 12-1 Kamishinano, Totsuka-ku, Yokohama-shi, Kanagawa Prefecture (56) References JP-A-10-287525 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A23L 1/28-1/308 A61K 7/00 JICST file (JOIS )

Claims (5)

(57) [Claims] 1. A food composition comprising proanthocyanidin and glutathione. 2. The composition according to claim 1, which is a whitening food. 3. The method according to claim 1, wherein the proanthocyanidin is contained in grape, blueberry, strawberry or avocado fruit or seed, or barley, wheat, red bean, soybean, black soybean, cacao or an extract thereof. 3. The composition of claim 1 or 2 wherein 4. The composition according to claim 1, wherein the glutathione is contained in yeast mycelium powder or yeast extract, or cow, pig or bird liver or an extract thereof. Stuff. 5. The composition according to claim 1, wherein the composition contains collagen or a collagen degradation product.