JP3043872B2 - Sprinkling filter type bioreactor using comb carrier - Google Patents
Sprinkling filter type bioreactor using comb carrierInfo
- Publication number
- JP3043872B2 JP3043872B2 JP30977691A JP30977691A JP3043872B2 JP 3043872 B2 JP3043872 B2 JP 3043872B2 JP 30977691 A JP30977691 A JP 30977691A JP 30977691 A JP30977691 A JP 30977691A JP 3043872 B2 JP3043872 B2 JP 3043872B2
- Authority
- JP
- Japan
- Prior art keywords
- comb
- carrier
- reactor
- filter type
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000855 fermentation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 12
- 239000007921 spray Substances 0.000 claims description 7
- 239000000919 ceramic Substances 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 210000004027 cell Anatomy 0.000 description 18
- 244000005700 microbiome Species 0.000 description 6
- 239000000969 carrier Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 4
- 230000003100 immobilizing effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 244000126211 Hericium coralloides Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000001822 immobilized cell Anatomy 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Biological Treatment Of Waste Water (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、基質を微生物に作用さ
せて有用物質を生成させるために用いられる散水濾床型
バイオリアクターの改良に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an improved trickling filter type bioreactor used for producing a useful substance by allowing a substrate to act on a microorganism.
【０００２】[0002]
【従来の技術】上記の目的に使用される散水濾床型バイ
オリアクターとしては、特開平２−２８４６９４号公報
に示されるように、多数の粒状担体をリアクター内にラ
ンダムパッキングしたものが知られている。ところがこ
のような粒状担体を用いたものは、菌体固定化量を増加
させて生産性を向上させようとして粒状担体の粒径を細
かくすると、菌体による粒子間の閉塞を完全に防止する
ことができない。また逆に粒径を大きくすると、閉塞は
防止できるものの粒子内部のデッドスペースが大きくな
り、リアクター全体の生産性が低下するという問題があ
った。2. Description of the Related Art As a trickling filter type bioreactor used for the above purpose, as shown in Japanese Patent Application Laid-Open No. 2-284694, a reactor in which a large number of granular carriers are randomly packed in a reactor is known. I have. However, in the case of using such a granular carrier, if the particle size of the granular carrier is reduced in order to increase the amount of cells immobilized and improve productivity, it is possible to completely prevent clogging between particles by the cells. Can not. Conversely, when the particle size is increased, clogging can be prevented, but the dead space inside the particles increases, and there is a problem that the productivity of the entire reactor decreases.
【０００３】[0003]
【発明が解決しようとする課題】本発明は上記した従来
の問題点を解決し、大きな菌体固定化量を維持しつつ菌
体による閉塞を完全に防止することができ、これによっ
て高い生産性を得ることができる櫛型担体を用いた散水
濾床型バイオリアクターを提供するためになされたもの
である。DISCLOSURE OF THE INVENTION The present invention solves the above-mentioned conventional problems, and can completely prevent cell clogging while maintaining a large amount of immobilized cells, thereby achieving high productivity. The purpose of the present invention is to provide a trickling filter type bioreactor using a comb-shaped carrier capable of obtaining the above.
【０００４】[0004]
【課題を解決するための手段】上記の課題を解決するた
めになされた本発明は、空隙率が30〜75％のセラミック
質からなり、厚みが２〜10mmの櫛歯を持つ微生物固定化
用の櫛型担体をリアクター内に積層し、その上方から醗
酵基質を散水することをスプレーノズルを設けたことを
特徴とするものである。SUMMARY OF THE INVENTION In order to solve the above-mentioned problems, the present invention provides a method for immobilizing microorganisms having a comb tooth having a porosity of 30 to 75% and a thickness of 2 to 10 mm. And a spray nozzle for spraying the fermentation substrate from above the comb-shaped carrier in a reactor.
【０００５】[0005]
【実施例】以下に本発明を図示の実施例に基づいて更に
詳細に説明する。図１において、１は縦長のリアクタ
ー、２はこのリアクター１の内部に積層された固定床ユ
ニット、３はリアクター１の上部に設けられたスプレー
ノズルである。このタイプのリアクターは嫌気発酵およ
び酸素要求量が比較的低い発酵に用いられる。また酸素
要求量が比較的多い発酵、または発酵液を循環しなけれ
ば生産物の濃度が高まらない発酵には、図２に示すよう
に底部から循環ポンプ３により循環液を吸い上げ、リア
クターの上部のスプレーノズル４から散水するタイプの
リアクターを用いる。以下の説明は図２のリアクターを
用いた実施例について行う。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in more detail with reference to the illustrated embodiments. In FIG. 1, reference numeral 1 denotes a vertically elongated reactor, 2 denotes a fixed floor unit laminated inside the reactor 1, and 3 denotes a spray nozzle provided on an upper portion of the reactor 1. This type of reactor is used for anaerobic fermentations and fermentations with relatively low oxygen demand. For fermentation with relatively high oxygen demand or fermentation in which the concentration of the product does not increase unless the fermentation liquor is circulated, the circulating liquid is sucked up from the bottom by the circulation pump 3 as shown in FIG. A reactor that sprays water from a spray nozzle 4 is used. The following description is made for an embodiment using the reactor shown in FIG.
【０００６】本発明の固定床ユニット２の内部には、図
３、図４に示すような微生物固定化用の櫛型担体５が多
数収納されている。これらの櫛型担体５は短い多数の櫛
歯６を備えたものであり、櫛歯６、６間に形成される溝
を垂直な流路７としている。閉塞を防止するためには多
段に積層された上下の櫛型担体５の垂直な流路７どうし
が一致していることが好ましい。図４の櫛型担体５では
櫛歯６の厚みと突出長さはともに５mmとなっている。A large number of comb-shaped carriers 5 for immobilizing microorganisms as shown in FIGS. 3 and 4 are accommodated in the fixed bed unit 2 of the present invention. These comb-shaped carriers 5 are provided with a large number of short comb teeth 6, and a groove formed between the comb teeth 6, 6 is a vertical flow path 7. In order to prevent clogging, it is preferable that the vertical channels 7 of the upper and lower comb carriers 5 stacked in multiple stages coincide with each other. In the comb carrier 5 shown in FIG. 4, both the thickness and the protruding length of the comb teeth 6 are 5 mm.
【０００７】本発明においては、櫛型担体５は空隙率が
30〜75％のセラミック質により構成されている。その理
由は図５に示されている。即ち、図５は空隙率の異なる
各種のセラミックスにより櫛型担体５を作成し、これを
リアクター１内にセットして１週間にわたり醗酵を行わ
せた後の全菌体量（櫛型担体５に固定化された菌体と醗
酵液中に浮遊している菌体の合計量）を左側の縦軸と
し、また櫛型担体５自体の圧縮強度を右側の縦軸として
示したグラフである。In the present invention, the comb-shaped carrier 5 has a porosity.
It is composed of 30-75% ceramic. The reason is shown in FIG. That is, FIG. 5 shows a comb-shaped carrier 5 made of various ceramics having different porosity, which was set in the reactor 1 and fermented for one week. This is a graph showing the total amount of the immobilized cells and the cells suspended in the fermentation solution) on the left vertical axis, and the compressive strength of the comb-shaped carrier 5 itself on the right vertical axis.
【０００８】まず菌体量は、全く担体を用いない通常の
連続醗酵の場合、リアクター内醗酵液の菌体の乾重量は
８g/L −リアクターであることから、菌体量がこの値を
上まわるようにしなければ担体を用いる意味がない。従
って担体の空隙率は30％以上とする必要がある。次に担
体のハンドリングの観点からは、圧縮強度が20kgf/cm2
以下では好ましくない。このため担体の空隙率の上限は
75％となり、以上の理由によって櫛型担体５の空隙率は
30〜75％、より好ましくは50〜70％とする。First, in the case of ordinary continuous fermentation without using any carrier, the dry weight of the cells in the fermentation broth in the reactor is 8 g / L-reactor. There is no point in using a carrier unless it is turned around. Therefore, the porosity of the carrier needs to be 30% or more. Next, from the viewpoint of carrier handling, the compressive strength is 20 kgf / cm 2
The following is not preferable. Therefore, the upper limit of the porosity of the carrier is
It is 75%, and the porosity of the comb-shaped carrier 5 is
30 to 75%, more preferably 50 to 70%.
【０００９】図６は櫛型担体５の櫛歯６の厚みとリアク
ター１内の菌体量との関係を示したグラフである。この
場合には、櫛歯６の厚みの異なる各種の櫛型担体５をリ
アクター１内に設置し、１週間醗酵を行った後のリアク
ター１内の全菌体量を測定した。前述したように、全く
担体を充填しない場合の菌体量は８g/L −リアクターで
あるから、櫛歯６の厚みを10mm以上とすると担体を用い
る意味がないことが分かる。これは厚みが大となると内
部に菌体が入り込めず、また仮に入ったとしても醗酵液
との接触ができないためである。逆に厚みが薄くなると
菌体量は増加するが、２mm以下の櫛歯６をセラミックに
より成形することは困難となるので、櫛歯６の厚みは２
〜10mmが好ましく、特に好ましくは３〜７mm程度とす
る。FIG. 6 is a graph showing the relationship between the thickness of the comb teeth 6 of the comb-shaped carrier 5 and the amount of cells in the reactor 1. In this case, various comb-shaped carriers 5 having different thicknesses of the comb teeth 6 were set in the reactor 1, and the total amount of cells in the reactor 1 after fermentation for one week was measured. As described above, when the carrier is not filled at all, the amount of cells is 8 g / L-reactor. Therefore, it can be understood that there is no point in using the carrier when the thickness of the comb teeth 6 is 10 mm or more. This is because when the thickness is large, the cells cannot enter the inside, and even if the cells enter the cell, they cannot contact the fermentation solution. Conversely, when the thickness is reduced, the amount of bacteria increases, but it is difficult to form the comb teeth 6 of 2 mm or less with ceramic.
It is preferably about 10 to 10 mm, particularly preferably about 3 to 7 mm.
【００１０】[0010]
【作用】このように構成された本発明の散水濾床型バイ
オリアクターは、空隙率が30〜75％のセラミック質から
なる櫛型担体５の表面に常法により微生物を固定化し、
栄養源を混ぜた基質をリアクター１内に供給して微生物
の作用により有用物質の生産を行わせるものである。そ
して生成された有用物質と基質との混合液である醗酵液
はリアクター１の底部に流下し、循環ポンプ４により吸
い上げられてスプレーノズル３から再び櫛型担体５上に
噴霧され、このような循環を繰り返しつつ次第に生産物
の濃度を向上させ、その一部は外部へ取り出される。The water-permeable filter type bioreactor of the present invention thus constructed has microorganisms immobilized on the surface of a comb-shaped carrier 5 made of ceramic having a porosity of 30 to 75% by an ordinary method.
A substrate mixed with a nutrient is supplied into the reactor 1 to produce a useful substance by the action of a microorganism. The fermented liquid, which is a mixture of the produced useful substance and the substrate, flows down to the bottom of the reactor 1, is sucked up by the circulation pump 4 and sprayed again from the spray nozzle 3 onto the comb-shaped carrier 5. , The concentration of the product is gradually increased, and part of the product is taken out.
【００１１】このように本発明においては醗酵液が櫛型
担体５の内部を常に循環することとなるが、櫛歯６の間
に垂直な流路７が確保されているので、菌体による担体
内部の閉塞が生ずるおそれがない。また櫛型担体５は空
隙率を30〜75％とし、また櫛歯６の厚みを２〜10mmとし
たので、十分な機械的強度を保ちながら多量の菌体を表
面層に固定化することができる。このためリアクター１
内の菌体量を高いレベルに維持して生産速度を向上させ
ることができる。As described above, in the present invention, the fermentation liquid always circulates inside the comb-shaped carrier 5, but since the vertical channel 7 is secured between the comb teeth 6, the carrier There is no risk of internal blockage. Since the porosity of the comb-shaped carrier 5 is set to 30 to 75% and the thickness of the comb teeth 6 is set to 2 to 10 mm, it is possible to immobilize a large amount of cells on the surface layer while maintaining sufficient mechanical strength. it can. For this reason, reactor 1
The production rate can be improved by maintaining the amount of cells in the cells at a high level.
【００１２】[0012]
【発明の効果】本発明は以上に説明したように、微生物
固定化用の担体形状を工夫することにより、菌体による
閉塞を防止しつつリアクター内の菌体量を高いレベルに
維持することができるものである。よって本発明は従来
の問題点を解消した櫛型担体を用いた散水濾床型バイオ
リアクターとして、産業の発展に寄与するところは極め
て大きいものである。As described above, according to the present invention, by devising a shape of a carrier for immobilizing microorganisms, it is possible to maintain the amount of cells in the reactor at a high level while preventing the cells from being blocked. You can do it. Therefore, the present invention greatly contributes to industrial development as a trickling filter type bioreactor using a comb-shaped carrier which has solved the conventional problems.
【図１】本発明の実施例を示す模式的な断面図である。FIG. 1 is a schematic sectional view showing an embodiment of the present invention.
【図２】本発明の他の実施例を示す模式的な断面図であ
る。FIG. 2 is a schematic sectional view showing another embodiment of the present invention.
【図３】櫛型担体の平面図である。FIG. 3 is a plan view of the comb carrier.
【図４】１枚の櫛型担体の斜視図である。FIG. 4 is a perspective view of one comb carrier.
【図５】櫛型担体の空隙率と菌体重量および担体の圧縮
強さとの関係を示すグラフである。FIG. 5 is a graph showing the relationship between the porosity of the comb-shaped carrier, the cell weight, and the compressive strength of the carrier.
【図６】櫛型担体の櫛歯の厚みと菌体重量との関係を示
すグラフである。FIG. 6 is a graph showing the relationship between the thickness of the comb teeth of the comb-shaped carrier and the cell weight.
１ リアクター ２ 固定床ユニット ３ スプレーノズル ４ 循環ポンプ ５ 櫛型担体 ６ 櫛歯 ７ 垂直な流路 DESCRIPTION OF SYMBOLS 1 Reactor 2 Fixed bed unit 3 Spray nozzle 4 Circulation pump 5 Comb carrier 6 Comb tooth 7 Vertical flow path
───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.7，ＤＢ名) C12M 1/00 - 3/00 C02F 3/04 - 3/28 ──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int. Cl. 7 , DB name) C12M 1/00-3/00 C02F 3/04-3/28
Claims (1)
り、厚みが２〜10mmの櫛歯を持つ微生物固定化用の櫛型
担体をリアクター内に積層し、その上方から醗酵基質を
散水することをスプレーノズルを設けたことを特徴とす
る櫛型担体を用いた散水濾床型バイオリアクター。1. A comb-shaped carrier made of ceramic having a porosity of 30 to 75% and having comb teeth having a thickness of 2 to 10 mm is laminated in a reactor, and a fermentation substrate is sprinkled from above the comb-shaped carrier. What is claimed is: 1. A sprinkling filter type bioreactor using a comb-shaped carrier, wherein a spray nozzle is provided.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30977691A JP3043872B2 (en) | 1991-10-28 | 1991-10-28 | Sprinkling filter type bioreactor using comb carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30977691A JP3043872B2 (en) | 1991-10-28 | 1991-10-28 | Sprinkling filter type bioreactor using comb carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05115277A JPH05115277A (en) | 1993-05-14 |
| JP3043872B2 true JP3043872B2 (en) | 2000-05-22 |
Family
ID=17997119
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP30977691A Expired - Lifetime JP3043872B2 (en) | 1991-10-28 | 1991-10-28 | Sprinkling filter type bioreactor using comb carrier |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3043872B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19919241A1 (en) * | 1999-04-28 | 2000-11-02 | Creavis Tech & Innovation Gmbh | Porous cell carrier system for preparing 3-D implants or explants and for cultivating connective tissue, comprises segments connected with a channel system |
| KR20020067090A (en) * | 2001-02-15 | 2002-08-22 | 주식회사 씨에스엔텍 | Sewage/waste water treating system |
| JP2007175686A (en) * | 2005-12-26 | 2007-07-12 | N Ii T Kk | Anaerobic sprinkling filtration method and apparatus for organic waste water |
-
1991
- 1991-10-28 JP JP30977691A patent/JP3043872B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH05115277A (en) | 1993-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1860223A (en) | Cell cultivation and breeding method. | |
| EP0130116A1 (en) | Transparent wall of polyurethane foam containing microorganisms, process for its preparation and its use in a biophotoreactor | |
| JP3043872B2 (en) | Sprinkling filter type bioreactor using comb carrier | |
| CN110217896A (en) | Microorganism embeds high-efficiency artificial wetland sewage treatment facility and technique | |
| JPS61227892A (en) | Method and apparatus for performing microbial fermentation | |
| JPH02284694A (en) | Trickling filter type bioreactor | |
| US12515971B2 (en) | Constructed wetland structure and methods for preparing and using carbon source | |
| CN108585177A (en) | A vertical flow enhanced biological water purification device and water purification method | |
| CN208532360U (en) | A vertical flow enhanced biological water purification device | |
| CN107758867A (en) | A kind of floating wetland purification system for efficiently removing waters eutrophication pollution | |
| CA1160171A (en) | Recirculation tower bioreactor for solid-state fermentation | |
| CN209890443U (en) | Aerobic-anaerobic alternate nitrogen and phosphorus removal constructed wetland with aeration system and water level control | |
| CN213977158U (en) | Probiotics product for keeping activity of oyster purification composite probiotics | |
| CN107325926B (en) | Application of two-way permeable membrane, solid-state liquor fermentation tank and solid-state liquor fermentation method | |
| CN113003708A (en) | Ammonia nitrogen removal mixed filter material, application thereof and constructed wetland | |
| JPH04267997A (en) | Reaction method of physiologically active substrate | |
| CN119240942B (en) | A method for enhancing nitrification and denitrification effects in a microbial purifier for aquaculture water | |
| CN221488477U (en) | Dendrobium nobile cultivation seedbed | |
| JPS63209788A (en) | Microorganism carrier | |
| JP3041378B2 (en) | Packed bed type culture apparatus and culture method | |
| JPS6013675B2 (en) | Production method of high concentration ethanol using immobilized bacteria | |
| CN108770771A (en) | A kind of purification system and fish jar of fish jar | |
| JPH0380467B2 (en) | ||
| JPS5847999Y2 (en) | Microbial breeding bed for organic wastewater treatment | |
| JPS6133293A (en) | Filter |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20000225 |