JP3050578B2 - Method and agent for controlling growth of blue-green algae - Google Patents
Method and agent for controlling growth of blue-green algaeInfo
- Publication number
- JP3050578B2 JP3050578B2 JP22983590A JP22983590A JP3050578B2 JP 3050578 B2 JP3050578 B2 JP 3050578B2 JP 22983590 A JP22983590 A JP 22983590A JP 22983590 A JP22983590 A JP 22983590A JP 3050578 B2 JP3050578 B2 JP 3050578B2
- Authority
- JP
- Japan
- Prior art keywords
- blue
- green algae
- saccharomyces
- algae
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Biological Wastes In General (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は富栄養化した湖やダムに発生しやすいアオコ
（青粉）の生育を抑制する方法および制御剤に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method and a control agent for suppressing the growth of blue-green algae (blue flour) which is likely to occur in eutrophic lakes and dams.
アオコは富栄養化した水中で主として植物性のプラン
クトンが異常増殖し、水面に薄皮あるいは塊状をなして
浮遊するもので、水面が緑色のじゅうたんを敷いたよう
になり、ダムに発生すれば給水に障害を起こすのみなら
ず、毒性もあるので水源として好ましくない。また多量
に発生すると水中溶存酸素が上層では過飽和となり下層
では欠乏するため魚類に被害を及ぼす。Blue-green algae are mainly phytoplankton that grow abnormally in eutrophic water and float in the form of skin or clumps on the surface of the water. It is not preferable as a water source because it is not only harmful but also toxic. If a large amount is generated, dissolved oxygen in water becomes supersaturated in the upper layer and becomes scarce in the lower layer, causing damage to fish.
アオコなどの抑制剤として従来一般に硫酸銅が使用さ
れている。そして藻類の 異常増殖が抑制されれば、富栄養化により魚介類などの
生産性は高まるが、銅イオンは毒性が強いので硫酸銅を
使用した水域の魚介類は食用に不適となる。Conventionally, copper sulfate has been generally used as a suppressant for blueberries and the like. If the overgrowth of algae is suppressed, eutrophication increases the productivity of fish and shellfish, but copper ions are highly toxic, and fish and shellfish in water bodies using copper sulfate are unsuitable for food.
本発明者は上記の課題を克服すべく研究を重ねた結
果、枯草菌または酵母や放緑菌の一部がアオコを沈澱さ
せ、あるいは殺藻的に作用することを見出した。As a result of repeated studies to overcome the above-mentioned problems, the present inventors have found that Bacillus subtilis or a part of yeasts and chlorophylls precipitates algae or acts algicidally.
本発明はこの知見に基づくもので、バチルス属、サッ
カロミセス属またはダクチロスポランジウム属に属し、
アオコを沈澱させうる微生物をアオコに接触させること
を特徴とするアオコの生育抑制方法、上記の微生物をア
オコの発生した水域に撒布することを特徴とするアオコ
の生育抑制方法、および上記の微生物を含有してなるア
オコの生育抑制剤である。The present invention is based on this finding, belongs to the genus Bacillus, Saccharomyces or Dactylosporandium,
A method for inhibiting the growth of blue-green algae, which comprises contacting microorganisms capable of precipitating blue-green algae with a blue-green algae, a method for inhibiting the growth of a blue-green algae, characterized by spraying the above-mentioned microorganisms on the water area where the blue-green algae occurred, and the microorganisms described above. It is a growth inhibitor of blue-green algae.
バチルス属、サッカロミセス属およびダクチロスポラ
ンジウム属の微生物はアオコを沈澱させもしくは殺藻す
ることができる限りいずれを用いてもよい。Any microorganisms of the genus Bacillus, Saccharomyces and Dactylosporandium may be used as long as they can precipitate or algae-kill the blue-green algae.
バチルス属の好ましい１例は枯草菌であり、それは本
発明者が分離し、工業技術院微生物工業技術研究所に寄
託番号FERM BP−1108を以て寄託している株を包含す
る。同株は水中の微細浮遊物を凝集する作用を示す。A preferred example of the genus Bacillus is Bacillus subtilis, which includes strains isolated by the present inventors and deposited with the Institute of Microbial Industry and Technology under the deposit number FERM BP-1108. The strain has the effect of aggregating fine suspended matter in water.
サッカロミセス属の例としては、本発明者が分離し、
同様に寄託した寄託番号FERM BP−3668のサッカロミセ
ス種を挙げることができる。同菌はサッカロミセスの性
状を備えるほか偽菌糸を多数形成する特徴を有する。Examples of Saccharomyces sp.
Similarly, a Saccharomyces sp. Having a deposit number of FERM BP-3668 may be mentioned. The bacterium has the characteristics of Saccharomyces and has the characteristic of forming many pseudohyphae.
ダクチロスポランジウム属の例としてはダクチロスポ
ランジウム・タイランデンス（Dactilosporundium tha
ilandense IFO 12593）が挙げられる。この菌は財団
法人発酵研究所の保存菌のリストに収載されている。An example of the genus Dactylosporundium is Dactilosporundium thalandens.
ilandense IFO 12593). This bacterium is listed on the list of conserved bacteria at the Fermentation Research Institute.
本発明の上記微生物をアオコに接触させると水面に浮
遊するアオコは水底に沈澱し、もしくはさらに死滅する
に至る。When the microorganisms of the present invention are brought into contact with blue water, the blue water floating on the water surface precipitates on the bottom of the water, or even die.
微生物とアオコの接触は、アオコの発生した水域、好
ましくは水面に微生物を撒布することにより有利に行う
ことができる。撒布は、たとえば、微生物の培養物を水
で希釈したけん濁液を撒布する形式で便宜に行うことが
できる。撒布には、必要に応じて、撒水機、噴霧機、吐
水ポンプなどを用いてもよい。The contact between the microorganisms and the blue-green can be advantageously performed by spraying the microorganisms on the water area where the blue-green has occurred, preferably on the water surface. Spraying can be conveniently performed, for example, by spraying a suspension obtained by diluting a culture of a microorganism with water. For spraying, a sprinkler, a sprayer, a water discharge pump, or the like may be used as necessary.
微生物の使用量は一般に104〜10個/cm3、好ましくは1
02個/cm3である。The amount of microorganism used is generally 10 4 to 10 cells / cm 3 , preferably 1
0 2 pieces / cm 3 .
撒布された微生物はアオコに付着してそのコロニー中
に侵入してその活力を低下させ、その結果アオコは浮力
を失って水底に沈澱する。沈澱したアオコは微生物の作
用により死滅する場合もあり、また水底の酸素が欠乏し
ているなどアオコの生育に不利な環境のために死滅する
場合もある。いずれにせよ、水面におけるアオコの繁殖
が阻害されるので水域の環境は改善され、水中動物の生
育に好影響を及ぼすことができる。The scattered microorganisms attach to the blue-green algae and invade their colonies, reducing their vitality. As a result, the blue-green algae lose buoyancy and settle on the water floor. Precipitated blue water may die due to the action of microorganisms, or may die due to an environment that is unfavorable to the growth of blue water, such as lack of oxygen at the bottom of the water. In any case, since the propagation of the blue-green algae on the water surface is inhibited, the environment of the water area is improved, and the growth of the underwater animals can be positively affected.
以下に実施例を挙げて本発明をさらに説明する。 Hereinafter, the present invention will be further described with reference to examples.
実施例１ 水源の沈澱湖にマット状に発生しているアオコを湖水
と一緒に採集し、各500ml容量の三角フラスコにアオコ
含有湖水300mlと菌液50ml（菌数５×103個）をそれぞれ
加え、アオコが水面に浮んだ状態で放置した。菌は枯草
菌（FERM BP−1108）、サッカロミセス種（FERM BP−
3668）およびダクチロスポランジウム（IFO 12593）を
用い、湖水のみで菌を加えないものを対照とした。結果
を次表に示す。Example 1 A blue-green algae, which has been generated in a mat on a sedimentary lake as a water source, is collected together with the lake water, and 300 ml of a blue-green algae-containing lake water and 50 ml of a bacterial solution (5 × 10 3 bacteria) are respectively collected in 500 ml Erlenmeyer flasks. In addition, the aoko was left floating on the surface of the water. The bacteria are Bacillus subtilis (FERM BP-1108), Saccharomyces sp.
3668) and dactylosporandium (IFO 12593) were used as controls, with only lake water containing no bacteria. The results are shown in the following table.
この試験における浮遊および沈澱したアオコの顕微鏡
写真を第１−４図に示す。 Photomicrographs of floating and sedimented blue-green algae in this test are shown in FIGS. 1-4.
第１図（×100）は対照において浮いているアオコで
大きな空泡が認められる。第２図Ａ（×100）および第
２図Ｂ（×400）はサッカロミセスにより沈澱したアオ
コで偽菌糸が多数認められる。沈澱しているにかかわら
ず空泡は残っている。第３図Ａ（×400）と第３図Ｂ
（×200）は枯草菌により沈澱したアオコで空泡が残っ
ているが、枯草菌がアオコのコロニーに入り込んでアオ
コの細胞を破壊している。第４図Ａ（×200）と第４図
Ｂ（×400）はダクチロスポランジウムにより沈澱した
アオコでコロニーは破壊されていないが、空泡は消失し
ている。In FIG. 1 (× 100), large air bubbles are observed in the blue-green algae in the control. In FIG. 2A (× 100) and FIG. 2B (× 400), a large number of pseudohyphae are observed in the blue-green algae precipitated by Saccharomyces. Despite the precipitation, air bubbles remain. Fig. 3A (x400) and Fig. 3B
(× 200) is a blue-green algae precipitated by Bacillus subtilis, with air bubbles remaining, but B. subtilis enters a blue-green algae colony and destroys the blue-green algae cells. FIG. 4A (× 200) and FIG. 4B (× 400) show that the colonies were not destroyed by the blue-green algae precipitated by dactylosporandium, but the air bubbles disappeared.
以上から明らかなように、いずれの菌にもアオコを沈
澱させる作用があるのみならず、枯草菌やダクチロスポ
ランジウム属菌には殺藻作用も認められ、特に後者は殺
藻作用が強いようである。As is clear from the above, not only all bacteria have the action of precipitating blue-green algae, but also Bacillus subtilis and bacteria of the genus Dactylosporandium have an algicidal effect. It is.
実施例２ 場所 台湾 時期 ３月10日−13日 池の面積（水深） 700坪（1.2m） アオコが発生した上記の池に枯草菌FERM BP−1108の
懸濁液（菌数108/ml）３を撒布した。実験開始前（３
月10日）と終了時（３月13日）の水質測定値を次表に示
す。Example 2 Location Taiwan Time March 10-13 Pond area (water depth) 700 tsubo (1.2 m) Suspension of Bacillus subtilis FERM BP-1108 (number of bacteria 10 8 / ml) 3) was sprayed. Before the start of the experiment (3
The following table shows the water quality measurements at the end of the month (March 10) and at the end (March 13).
〔発明の効果〕 本発明によればバチルス属、サッカロミセス属または
ダクチロスポランジウム属の微生物をアオコに接触させ
るという簡易な手段によりアオコの生育を抑制してアオ
コ発生水域の環境を改善することができる。 (Effect of the Invention) According to the present invention, it is possible to suppress the growth of blue-green algae by a simple means of contacting a microorganism of the genus Bacillus, Saccharomyces or Dactylosporandium with the blue-green algae, and to improve the environment of the blue-green algae-producing water area. it can.
図面は実施例１の実験におけるアオコの形態をあらわす
顕微鏡写真で、第１図（×100）は対照のアオコ、第２
図Ａ（×100）およびＢ（×400）はサッカロミセス属菌
により沈殿したアオコ、第３図Ａ（×200）およびＢ
（×400）は枯草菌による沈殿したアオコ、第４図Ａ
（×200）およびＢ（×400）はダクチロスポランジウム
属菌により沈殿したアオコ、の夫々の形態をあらわすも
のである。The drawing is a photomicrograph showing the form of the blue-green algae in the experiment of Example 1. FIG.
Figures A (x100) and B (x400) show blue-green algae precipitated by Saccharomyces sp., FIG. 3 A (x200) and B
(× 400) is blue-green algae precipitated by Bacillus subtilis, FIG. 4A
(× 200) and B (× 400) represent the respective forms of blue-green algae precipitated by Dactylosporandium.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ (Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:07） (Ｃ１２Ｎ 1/20 Ｃ１２Ｒ 1:01） ──────────────────────────────────────────────────の Continued on front page (51) Int.Cl. 7 Identification symbol FI (C12N 1/20 C12R 1:07) (C12N 1/20 C12R 1:01)
Claims (5)
チロスポランジウム属に属し、アオコを沈澱させもしく
は殺藻しうる微生物をアオコに接触させることを特徴と
するアオコの生育抑制方法。The present invention relates to a method for inhibiting the growth of blue-green algae, which comprises contacting a micro-organism belonging to the genus Bacillus, Saccharomyces or the genus Dactylosporandium with the ability to precipitate or algae-killing the blue-green algae.
チロスポランジウム属に属し、アオコを沈澱させもしく
は殺藻しうる微生物の株をアオコの発生した水域に撒布
することを特徴とするアオコの生育抑制方法。2. A method for suppressing the growth of blue-green algae, which comprises spraying a strain of a microorganism belonging to the genus Bacillus, Saccharomyces or Dactylosporandium, which is capable of precipitating or algae-killing the blue-green algae on a water area where the blue-green algae is generated. .
Ｐ−5622）またはダクチロスポランジウム・タイラン
デンスである請求項１または２記載の方法。3. The method according to claim 2, wherein the microorganism is Bacillus subtilis, Saccharomyces sp.
The method according to claim 1 or 2, which is P-5622) or Dactylosporandium tylandens.
チロスポランジウム属に属し、アオコを沈澱させもしく
は殺藻しうる微生物を含有してなるアオコの生育抑制
剤。4. A growth control agent for blue-green algae comprising a microorganism belonging to the genus Bacillus, Saccharomyces or Dactylosporandium and capable of precipitating or algae-killing the blue-green algae.
Ｐ−5622）またはダクチロスポランジウム・タイラン
デンスである請求項４記載の抑制剤。5. The method according to claim 5, wherein the microorganism is Bacillus subtilis, Saccharomyces sp.
The inhibitor according to claim 4, which is P-5622) or dactylosporandium tylandens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22983590A JP3050578B2 (en) | 1990-08-30 | 1990-08-30 | Method and agent for controlling growth of blue-green algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22983590A JP3050578B2 (en) | 1990-08-30 | 1990-08-30 | Method and agent for controlling growth of blue-green algae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04110087A JPH04110087A (en) | 1992-04-10 |
| JP3050578B2 true JP3050578B2 (en) | 2000-06-12 |
Family
ID=16898422
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP22983590A Expired - Fee Related JP3050578B2 (en) | 1990-08-30 | 1990-08-30 | Method and agent for controlling growth of blue-green algae |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3050578B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8699738B2 (en) | 2010-12-03 | 2014-04-15 | Panasonic Corporation | Speaker system with resonance frequency approximately identical to the peak frequency of the sound pressure |
| JP6538304B2 (en) * | 2013-01-22 | 2019-07-03 | 東和酵素株式会社 | Water purification method in closed water system using microorganisms |
| CN110104797A (en) * | 2019-05-30 | 2019-08-09 | 广东子辉生态环境科技有限公司 | A kind of application method for the Tiny ecosystem flora except algae |
-
1990
- 1990-08-30 JP JP22983590A patent/JP3050578B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04110087A (en) | 1992-04-10 |
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