JP3121850B2 - Method for isolating acid secretion inhibitor - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel acid secretion inhibitor isolated from a medicinal carrot or a tissue culture thereof, and a method for isolating the same.

[0002]

2. Description of the Related Art Medicinal carrots, for example, Panax ginseng CAMeyer, are known to have a pharmacological effect such as an anti-fatigue effect, an anti-stress effect, and a metabolic hyperactivity. It is also useful for improving gastrointestinal function. It is considered that such improvement in gastrointestinal function is due to the action of medicinal carrots to suppress acid secretion. Ginsenoside, a kind of saponin, has been reported as an active ingredient for inhibiting the secretion of acid by medicinal carrots, but other components have not been elucidated. If other carrot-derived substances having an acid secretion action could be obtained, it would be possible to provide new drugs using the same.

[0003]

DISCLOSURE OF THE INVENTION The object of the present invention is to solve the above-mentioned conventional problems, and an object of the present invention is to provide an acid secretion inhibitor other than saponin contained in a medicinal carrot, And a method for isolating the acid secretion inhibitor.

[0004]

The method for isolating an acid secretion inhibitor according to the present invention comprises a step of extracting a medicinal carrot or a tissue culture thereof or a dried product thereof with an aqueous organic solvent; Precipitating with an organic solvent and collecting the precipitate; and subjecting the precipitate to ultrafiltration to give a molecular weight of 14
Collecting the low molecular fraction of 000 or less, thereby achieving the above object.

The medicinal carrots used in the present invention include Panax ginseng C.A. Meyer, Panax japonicus CAMeyer, Panax quinquefolium L., Panax notoginseng (Burk.) FHChen, Himalayan carrot ( Panax pseudo-ginseng Wall. Subsp. Himalaicu
sHara), ginseng ( Panax japonics CAMeyer var. maj)
or (Burk.) CYWu et KMFeng), Panax zi
ngiberenesis CYWu et KMFeng), Heihen thirty-seven (Panax
stipuleanatus Tsai et Feng), Semaha Takefushi ginseng (Panax j
aponics CAMeyer var.angustifolitus (Burk.) Chen
g et Chu). The root of these medicated carrots or a dried product thereof is used. Such a dried product can be obtained, for example, as a Chinese medicine such as white ginseng or red ginseng. Alternatively, a medicinal carrot tissue culture can be used. Tissue cultures can be prepared by conventional methods. For example, a part of the medicinal carrot tissue is aseptically cultured in a solid medium containing a plant hormone to generate callus. Culture conditions need not be exceptional. As the medium, Murashige which is usually used for tissue culture of plants is used.
Skoog's medium, White medium, Rinsmeyer-Skoog's medium, Gausslet's medium, Heller's medium, Gamborg's medium, Nitsche-Nitsche's medium and modified media thereof can be used. The callus is further transferred to a liquid or solid medium and cultured to prepare a desired tissue culture. In particular, a tissue culture of Panax ginseng is preferably used.

According to the method of the present invention, an acid secretion inhibitor is isolated as follows. First, the above-mentioned medicinal carrot or its tissue culture is dried if necessary. This is extracted with an aqueous organic solvent. As the aqueous organic solvent, an aqueous solution containing 40 to 80% of at least one water-miscible organic solvent such as methanol, ethanol, and acetone is used. Alternatively, water may be used alone. At the time of extraction, it is preferable to pulverize the medicinal carrot or to heat the extraction mixture. Such an operation facilitates the extraction. However, the temperature at the time of extraction preferably does not exceed 80 ° C.

Next, the above extract is filtered, and the filtrate is concentrated under reduced pressure. And after suspending this concentrate in water,
An organic solvent is added for precipitation, and the precipitate is collected. As an organic solvent used for this precipitation treatment, methanol,
A water-miscible solvent such as ethanol or acetone is preferably used.

[0008] The precipitate obtained above is suspended again in water and fractionated using an ultrafiltration membrane to recover a low-molecular fraction (molecular weight of 14,000 or less). When this fraction was concentrated under reduced pressure and subjected to thin layer chromatography, spots were observed at positions different from those of saponin.

When the thus obtained non-saponinous substance is mixed with a suitable auxiliary agent (for example, a hot water extract of licorice, white jujube and dried ginger, etc.), a very strong central acid is obtained. Secretion inhibitory effect was obtained. That is, the non-saponin substance obtained here is a novel acid secretion inhibitor, and an excellent acid secretion inhibitor can be obtained by blending it with an appropriate auxiliary. Furthermore, the acid secretion inhibitor is harmless to the human body because it uses medicinal carrot as a raw material, and the acid secretion inhibitor using the same can be administered as an oral medicine having an antiulcer effect.

[0010]

The present invention will be described with reference to the following examples.

A. Isolation of acid secretion inhibitor A part of the tissue of Panax ginseng CAMeyer was aseptically cultured on an agar medium containing plant hormones to generate calli.
Cultured for days. The obtained callus was dried to obtain a dry callus of 40 kg. This dried callus is added to a 50% ethanol aqueous solution
After placing in 72 liters and heating at 70 ° C. for 1 hour, the extract was filtered. To the residue was further added 72 liters of a 50% aqueous ethanol solution, followed by heating, extraction and filtration in the same manner. The obtained filtrate was combined with the previous filtrate and concentrated under reduced pressure.
7,480 g of extract was obtained. 940 g of this extract was suspended in 5 times the volume of water, and the suspension was added with 5 times the volume of ethanol and left overnight. The precipitated precipitate was collected by filtration and the solvent was distilled off to obtain 550 g of a precipitate. The precipitate was collected, suspended in 3 liters of water, and the suspension was filled in a cellulose dialysis tube having a molecular weight cutoff of 12,000 to 14,000, and dialyzed against 45 liters of water for 1 day. Was. The same operation was performed three times while replacing the water. The obtained outer dialysates were combined and concentrated under reduced pressure to obtain 523 g of a concentrate. When the concentrate was subjected to thin-layer chromatography, a single spot was found at a position different from that of saponin.

B. Assay of acid secretion inhibitory effect The Wistar strain male rat (body weight: 200 to 300 g) was used to assay the acid secretion inhibitory effect by the intragastric perfusion method.

[0013] 934 g of the dialysis external solution obtained in the above section A was added to 1000 g of a hot water extract of licorice, white jujube and dried ginger and mixed to obtain a test solution (A). For comparison, a physiological saline solution (control) (B); a hot water extract of licorice, white jujutsu and ginger (C); and a solution (D) containing atropine sulfate, a known acid secretion inhibitor, were used. Similarly, the following tests were performed.

The rat stomach was perfused with physiological saline kept at 37 ° C. at a rate of 1 ml / min, and the test solution or the comparative substance solution was administered into the duodenum. 200 mg / kg of glucose was administered subcutaneously. Collect the perfusate for 2 hours from the administration of 2-deoxyglucose and
Titration was performed with NaOH until the pH became 7.0 to 7.4, and the amount of secreted acid was measured. Table 1 shows the results.

[0015]

[Table 1]

As is clear from Table 1, the substance obtained by the method of the present invention has an acid secretion inhibitory action comparable to that of atropine sulfate.

[0017]

According to the method of the present invention, a substance having an inhibitory action on acid secretion can be easily isolated from medicinal carrot or its tissue culture. The acid secretion inhibitor obtained by the present invention can be widely used, for example, as a preventive or therapeutic drug for ulcer.

Claims (3)

(57) [Claims] 1. a step of extracting a medicinal carrot or a tissue culture thereof or a dried product thereof with an aqueous organic solvent; a step of precipitating the obtained extract with an organic solvent and collecting a precipitate; Subjecting the substance to an ultrafiltration treatment to collect a low-molecular fraction having a molecular weight of 14,000 or less . 2. The medicinal carrot is a panax ginseng ( Pa)
nax ginseng CAMeyer), Panax jap
onicus CAMeyer), American carrot ( Panax quinqu)
efolium L.), Panax notoginseng (Bur
k.) FHChen), Himalayan carrot ( Panax pseudo-gins)
eng Wall. subsp. himalaicus Hara) , Tamako ginseng (Panax
japonics CAMeyer var.major (Burk.) CYWu et K.
M. Feng), Panax zingiberenesis CYWu et
KMFeng), Panax stipuleanatus Tsai et
Feng) and Bamboo Ginseng Saban ( Panax japonics CAMeye)
The method according to claim 1, wherein the method is selected from the group consisting of r var. angustifolitus (Burk.) Cheng et Chu). 3. An acid secretion inhibitor isolated by the method according to claim 1.