JP3280650B2 - Visualization judgment method of soil containing protein of plumbing goods - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for visualizing and judging protein-containing dirt, and more particularly to a toilet, kitchen,
The present invention relates to a method for visualizing and judging dirt on equipment in a plumbing space such as a bathroom.

[0002]

2. Description of the Related Art Around a water space, for example, a wall of a toilet, a kitchen, a washroom, a bathroom, etc.
It is desirable that indoor sanitary facilities such as ceilings, floors, toilets, washbasins, kitchen sinks, etc., be kept clean at all times to maintain good sanitary conditions. Walls, ceilings, floors and indoor sanitary facilities of these plumbing sanitary rooms, particularly indoor sanitary facilities, are usually cleaned at regular intervals so as to keep them in a clean state.

In recent years, various types of sanitary facilities which have been subjected to antibacterial treatment have been developed. In the development, the extent of the antibacterial effect, that is, the extent to which the generation and proliferation of bacteria and mold on the sanitary facilities surface is suppressed. There is a need for a method for clearly determining whether the contamination has occurred, that is, how easily dirt is generated. In addition, there is a need for a method that can clearly determine the degree of adhesion of dirt for each room constituent material such as the wall, ceiling, floor, and the like, and for each material (plastic, metal, pottery, etc.) forming the indoor equipment.

Conventionally, the determination of dirt on the surface of these sanitary facilities and the like has been made based on observation of the state of adhesion of water droplets and the state of wetness of water when cleaning is performed. It is difficult to clearly determine the state of adhesion and its degree.

Conventionally, as a method for determining protein-containing stains, in the food-related field, the surface of a food container or the like has to be rubbed, and the stain has to be put into a reagent which reacts with the protein to form a color, and the degree of color formation has been checked. A method for determining the presence or absence and degree of contamination is known in Japanese Patent Application Laid-Open No. Hei 8-21837.
issue).

[0006] Alternatively, a dye which reacts with a protein and develops a color and is added with a surfactant is applied to the surface of the object to produce a color due to the reaction between the dye and the protein. Judgment was made so that this could be used as a target for washing with a surfactant.
No. 699.

However, since these methods all visualize protein-containing stains by coloring, the color of the object, for example, in the case of a wash basin, the color of the wash basin impairs the sharpness of color development. At the same time, there is a problem that the coloring is not sufficiently sensitive to the stain, and therefore, it is difficult or impossible to determine the stain particularly when the stain is minute.

[0008]

The invention of the present application has been made to solve such a problem. According to a first aspect of the present invention, there is provided a method for judging visualization of dirt, wherein the water, sanitary room wall, ceiling, or floor of any one of a toilet, a kitchen, a washroom, and a bathroom and any one of indoor facilities of the sanitary room. A test piece of a surrounding product is prepared, the test piece is subjected to a fluorescent reagent on the surface of the test piece as a target for determination of contamination, and the protein on the test piece surface is reacted with the fluorescent reagent, and then irradiated with ultraviolet light. The degree of contamination is determined by observing the fluorescence intensity from the surface of the test piece.

According to a second aspect of the present invention, in the first aspect, the test piece before use is attached to an already installed same type of plumbing product, and the plumbing product is used. The method is characterized in that a fluorescent reagent is applied to the surface of the test piece to determine the degree of contamination.

In a third aspect of the present invention, the method for determining visualization of dirt is characterized in that in any one of the first and second aspects, fluorescamine or vanillin is used as the fluorescent reagent.

According to a fourth aspect of the present invention, in the method for determining visualization of dirt, the fluorescent reagent according to any one of the first to third aspects is spray-sprayed on the surface of the plumbing product to react with the dirt containing the protein. And

[0012]

As described above, the method for determining the visualization of dirt according to the first aspect is to apply a fluorescent reagent to the surface of a test piece of a water-applied product, irradiate ultraviolet rays, and observe the fluorescence intensity from the surface. In the case of this visualization determination method for dirt, unlike the conventional case in which a protein and a coloring reagent are reacted with each other to form a dirt containing protein, the determination of dirt is not performed. It is not influenced by the color of the test piece itself in the plumbing product, and it is possible to detect minute dirt of about 10 ng, and it is possible to clearly determine even minute dirt.

According to the present invention, when judging the contamination of a plumbing product according to the present invention, a test piece of the material is prepared, and the judgment of the contamination is performed on the test piece. Can be easily heated when necessary.

[0014] Next, according to a visualization judgment method of the present invention, the test piece is attached to the same kind of water-supplied article already installed, and the water-supplied article is used. After use, a fluorescent reagent is applied to the surface of the test piece. To determine the degree of dirt. When applying the present invention, it is possible to visualize and judge stains by spraying a fluorescent reagent with the material as it is, but it is also possible to make a test piece with such a material and attach the test piece to a basin bowl or other product To evaluate the degree of dirt.

For example, when a new bathtub is formed using a resin, when a bathtub is formed from the material and the degree of contamination is determined, a bathtub mold is formed at a large cost, and the bathtub is formed. Then it is necessary to test. However, by preparing a test piece of an appropriate size, pasting this to an existing bathtub and using the bathtub, by visualizing and determining the degree of contamination of the test piece after use,
It is possible to evaluate whether or not the material is easy to generate stains.

Various fluorescent reagents can be used, and fluorescamine or vanillin is particularly preferably used (claim 3). When vanillin is used as the fluorescent reagent, the reaction can be sufficiently performed by applying the vanillin to the surface of the object and then heating it to around 110 ° C. In the case of the former fluorescamine, the reaction can be performed at room temperature. Can react with proteins,
It is particularly suitable as a fluorescent reagent to be used.

When the fluorescent reagent is applied to the surface of the object, it is desirable to spray the fluorescent reagent on the surface of the object. In this way, the fluorescent reagent can be easily and uniformly applied to the surface of the object over a wide range, and thus the surface of the object, that is, the surface of the test piece of the plumbing product can be easily visualized and determined. be able to.

In the present invention, the indoor equipment includes the following. That is, examples of indoor facilities of toilets include other toilets made of ceramics such as toilets and urinals, resin toilet lids and resin toilet seats attached to the toilets, and other hand washers made of ceramics and washing water tanks. The kitchen facilities include kitchen sink,
Water faucet fittings, ceramic tiles constituting kitchen walls, kitchen counters and the like can be mentioned.

Further, examples of the indoor facilities of the washroom include other basins and counters made of ceramics, and examples of the indoor facilities of the bathroom include a bathtub, a bathroom counter, and a faucet fitting. In addition, a ceramic tile or the like constituting a wall or the like of each plumbing sanitary room is regarded as one plumbing product, and the stain on the surface can be determined by causing the fluorescent reagent to emit fluorescent light according to the present invention.

[0020]

Next, embodiments of the present invention will be described in detail. <Example 1> 18 g of ordinary bouillon (Eiken Chemical Co., Ltd.) as a substance equivalent to protein (containing -NH ₂ ) as a dirt component was dissolved in 1000 ml of distilled water to prepare a stock solution. 50, 1/500, 1/1
000 diluted to the surface of a test piece made of sanitary ware was inoculated in an amount of 0.5 ml each and dried.

The composition of the stock solution is as follows per 1000 ml. Stock comprising extract 3g Peptone 10g sodium chloride 5g this meat extract, peptone, sodium chloride is a substance that contains -NH _2.

Next, the above stock solution, 1/50 diluent, 1/50
Fluorescamine and ninhydrin were sprayed on the surface of the test piece inoculated with the 0 dilution and the 1/1000 dilution. In addition, what sprayed ninhydrin was heat-processed at 110 degreeC after that for 10 minutes.

Here, fluorescamin (fluorescamin)
e) is a fluorescent reagent represented by the chemical formula of C ₁₇ H ₁₀ O ₄ (it does not emit fluorescence by itself), and as shown in the following formula (1), a protein (specifically, an amino group of a protein, Reacts with the amino group of the primary amine) to produce a fluorescent substance.

[0024]

Embedded image

In this example, 0.025 g of fluorescamine was used as the solvent in dimethyl sulfoxide 10
The solution dissolved in 0 ml was used. The spray amount on the surface of the sample was 0.01 to 0.20 g per 1 cm ² . A desirable spray amount is 0.03 to 0.15 g, and a more desirable spray amount is 0.03 to 0.12 g.

After the spray treatment of fluorescamine and ninhydrin as described above, irradiation with ultraviolet rays (wavelength 36
5 nm), and the state of the sample surface was observed.
As a result, the fluorescamine sprayed showed fluorescence and the fluorescence intensity was strong in the order of 1/1000 diluted solution → 1/500 diluted solution → 1/50 diluted solution → stock solution. It should be noted that ninhydrin reacts with proteins to develop color and does not generate fluorescent substances.

With respect to the ninhydrin-treated product, only the solution inoculated with the undiluted solution and the 1/50 diluted solution without irradiation with ultraviolet rays produces a color that can be discerned by the naked eye, and the degree of the color development is naturally inoculated with the undiluted solution. Is strong,
The degree of color development was weak for the 1/50 dilution. No color development was observed with the naked eye for the inoculated 1/500 diluted solution and 1/1000 diluted solution.

Tables 1 and 2 show the results. Here, Table 1 shows the results of visual confirmation without irradiation with ultraviolet rays, and Table 2 shows the results of visual confirmation with irradiation of ultraviolet rays.

[0029]

[Table 1]

[0030]

[Table 2]

In this test, the surface state of the fluorescamine and ninhydrin which were not spray-treated (non-spray-treated product) was similarly observed. / 50 diluent, 1/500
Regarding the diluent and the 1/1000 diluent, it was not possible to sufficiently confirm that the surface state changed according to the degree of dirt (the amount of inoculated broth).

As is clear from the results shown in Tables 1 and 2, in the method of judging dirt by spraying ninhydrin to form a color, when the degree of dirt becomes smaller, this becomes clearer. Although it cannot be visually confirmed, in the case of the method of the present embodiment in which fluorescamine is sprayed and irradiated with ultraviolet rays to cause the sample surface to emit fluorescent light to determine the degree of dirt, even if the degree of dirt is slight, it can be clearly determined. Can be visually confirmed and determined.

<Example 2> A basin bowl of an existing men's toilet was treated according to the following conditions, and dirt adhering thereto was investigated using fluorescamine as a fluorescent reagent. Specifically, half of the existing basin bowl (left half)
Was washed under the following conditions, and while the other half (right half) was left as it was, fluorescamine was sprayed and irradiated with ultraviolet rays, and a photograph was taken to observe the state of dirt. The basin bowl has been estimated for about 10 hours since the last cleaning.

[Test conditions] Target basin bowl A basin bowl with office men's toilet (approximately 1 after cleaning)
0 hours) ・ Pretreatment of spraying fluorescamine The left half of the bowl is washed with a neutral detergent and tap water using a nylon scrubber → rinse with distilled water → dry with a dryer → use tap water with a faucet equipped with a basin. Rinse → Rinse with distilled water → Dry with dryer ・ Spraying fluorescent reagent Fluorescamine in dimethyl sulfoxide (0.2
(5 g / liter) was sprayed uniformly over the entire inner surface of the basin bowl with a sprayer, and allowed to stand for 30 minutes to perform fluorescence imaging.・ Fluorescence photography Illumination: 365 nm UV light source side filter: UV cut filter Camera side filter: UV cut filter Camera used: Olympus OM-2 Exposure time: 1 minute Aperture: 3.5

As a result, the right half of the basin bowl that had not been subjected to the cleaning treatment was entirely contaminated, had poor surface gloss, and repelled water. After applying the fluorescamine solution, the surface condition was photographed under ultraviolet irradiation. As a result, the right half of the basin bowl was strongly fluoresced, and the photograph was whitish.
That is, after applying fluorescamine, taking a photograph under ultraviolet irradiation, the state of dirt could be clearly seen.

In Examples 1 and 2, fluorescamine can of course be used in combination with a solvent other than the above examples or at a different concentration. For example, it may be used in a combination of fluorescamine 0.025 g / dimethylformamide 100 ml, or fluorescamine 0.050 g / dimethylsulfoxide 100
fluorescamine 0.050g
/ Dimethylformamide 100 ml combination or other combinations.

Here, 0.025 g of fluorescamine was added.
Dimethyl sulfoxide in 0.050 g, twice the amount of
When used by dissolving in 100 ml of a solvent such as dimethylformamide, a sufficient amount of fluorescamine that reacts with dirt is supplied even when the spray amount on the surface of the object is small, so that a sufficient reaction can be obtained. .

Here, no problem arises even if fluorescamine is supplied in excess to the surface of the object. This excess of fluorescamine, which is not used in the reaction, can be easily hydrolyzed to form a water-soluble non-fluorescent substance, which can be discharged together with water.

When a solvent that has been subjected to a non-fluorescent treatment (does not emit fluorescence even when irradiated with ultraviolet rays or the like) is used as a solvent, the fluorescence due to the reaction with fluorescamine is more remarkably advantageous.

In the above embodiment, the ultraviolet wavelength is 365 n
m, but 360, 3
The wavelength may be changed near 360 nm, such as 65,366 nm, or a wavelength of 254 nm or near may be used.

The method of the present invention is suitable not only as a method for testing a molded product itself, but also as a method for determining the visualization of stains on the material of the product. That is, when applying the present invention, it is possible to visualize and judge stains by spraying a fluorescent reagent with the material as it is, but a test piece is prepared from such a material, and the test piece is attached to a basin bowl or other product. It is also possible to evaluate the degree of dirt.

More specifically, when a new bathtub is manufactured using a resin, the degree of dirt is determined after the bathtub is manufactured from the material. Need to be molded and then tested. However, prepare a test piece of appropriate size, stick it to the existing bathtub and use the bathtub,
After the use, the degree of dirt on the test piece is visualized and determined by the method of the present invention to evaluate whether the material is easy to generate dirt or hard to generate dirt. Can be.

Further, when the stain is visualized and determined by using such a test piece, there is an advantage that, for example, the reagent can be sprayed inside the draft or heating can be easily performed when heating is required. can get.

Although the above is an example of the case of fluorescamine, in the present invention, vanillin can be used as a fluorescent reagent. That is, the present inventor sprayed and sprayed this using vanillin as a fluorescent reagent, and then performed a test in which the reaction was heated to about 110 ° C. and then irradiated with ultraviolet light to examine the fluorescent intensity. It could be confirmed visually.

The embodiment of the present invention has been described in detail above, but this is merely an example. For example, in photography, it is also possible to attach a filter that transmits only the fluorescence wavelength to the camera and take a picture. In this case, the resulting photo shows the fluorescent part but most of the sample is reflected by adjusting the exposure time. Since it is possible to create a situation in which the fluorescent portion is not present, it is possible to further enhance the fluorescent portion. In addition, the present invention digitizes the fluorescence emission from the surface of the object by image processing, and it is also possible to judge dirt based on the numerical value. It is feasible.

 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Hiroyuki Miyamoto 5-1-1 Koiehonmachi, Tokoname-shi, Aichi Prefecture Inax Corporation (72) Inventor Akimitsu 5-1-1, Koiehonmachi, Tokoname-shi, Aichi Prefecture Stock Company Inax Corporation (56) References JP-A-61-164158 (JP, A) JP-A-5-24043 (JP, U)

Claims (4)

(57) [Claims] 1. A test piece of a plumbing product of any of a wall, a ceiling, a floor of a plumbing room of a toilet, a kitchen, a washroom, and a bathroom and indoor equipment of the plumbing room, A fluorescent reagent is applied to the surface of the test piece with the test piece as a determination target of contamination, and the protein on the test piece surface is reacted with the fluorescent reagent, and then irradiated with ultraviolet light, and the fluorescence intensity from the test piece surface is observed. A method for determining the degree of dirt by performing the visualization of dirt containing protein of a plumbing product. 2. The test piece according to claim 1, wherein the test piece before use is attached to the same type of water-supplied article already installed, and the water-supplied article is used. After use, a fluorescent reagent is applied to the surface of the test piece. A method for determining the degree of contamination by determining the degree of contamination by visualizing the contamination including protein of a plumbing product. 3. The method according to claim 1, wherein fluorescamine or vanillin is used as the fluorescent reagent. 4. The plumbing product according to claim 1, wherein the fluorescent reagent is sprayed onto the surface of the plumbing product to react with the dirt containing the protein. Soil visualization determination method.