JP3317901B2 - Lipid peroxide inhibitor for epilepsy and method for producing the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to a lipid peroxide inhibitor effective for traumatic epilepsy using Hakutsuru Reishi and a method for producing the same.

[0002]

2. Description of the Related Art Traumatic epilepsy generates active oxygen,
It is said to increase lipid peroxide in the cerebral cortex. This lipid peroxide induces a wide range of membrane damage such as disruption of membrane structure, and has the adverse effect of secondary production of cytotoxicity [Koji Uchida, “Changes in Proteins by Lipid Oxide”, edited by Masayasu Inoue, Active Oxygen And Kyokuryu Shuppan (1996)]. In order to eliminate such disorders caused by traumatic epilepsy, a method of administering a drug which suppresses the production of lipid peroxide by radical treatment with an antiepileptic drug or directly has been adopted. For example, a study by Komatsu et al. On the study of the antiepileptic drug zonisamide on the lipid peroxide inhibitory effect [Komatsu et al. "On the lipid peroxide inhibitory effect of zonisamide" NEUROSCI
ENSES, May, 1995, Vol. 21, P63
-P66] is intended for radical treatment, i.e., suppression of lipid peroxides by treating epilepsy.

[0003] Rinacanthus n
asuta (L.) Kurz) is an evergreen shrub belonging to the linac family of the genus Linacanthus, which is native to the Deccan Plateau in southern India. (Primary Color Makino Kazoku Herb Encyclopedia, p. 492, Hokuryukan, 1
988), mainly in China, Taiwan, etc., and recently also in Japan. In addition, in a previous application filed by the present applicant, this white cranes Reishi had the ability to scavenge active oxygen (Japanese Patent Application Laid-Open No. 9-143091, a composition having the ability to scavenge active oxygen) and the effect of promoting excretion (Japanese Unexamined Patent Application Publication No. No. 9-169662, excretion enhancer). However, it is not known that the water and / or organic solvent extract of Hakutsuru Reishi or Hakutsuru Reishi has an effect of inhibiting lipid peroxide.

[0004]

DISCLOSURE OF THE INVENTION The present inventors have conducted intensive studies on substances that suppress the production of lipid peroxides due to traumatic epilepsy, and as a result, have found that white crane spirits, which have been used as a traditional Chinese medicine, an excretion enhancer, and a health food. The inventors have found that turf water and / or organic solvent extracts are effective, and have completed the present invention.

[0005]

DISCLOSURE OF THE INVENTION The present invention is a lipid peroxide inhibitor for epilepsy characterized by containing a water and / or organic solvent extract of Hakutsuru Reishi as a main component. Further, the present invention is a lipid peroxide inhibitor for epilepsy, characterized in that the extract contains water and / or an organic solvent extract of Hakutsuru Reishi. Further, the present invention is a lipid peroxide inhibitor for epilepsy, characterized by comprising as a main component a fine powder obtained by treating water and / or an organic solvent extract of Hakutsuru Reishi. The present invention also relates to a method for producing a lipid peroxide inhibitor for epilepsy comprising a water and / or organic solvent extract of Hakutsuru Reishi, wherein Hakutsuru Reishi is extracted with hot water. A method for producing a lipid peroxide inhibitor for epilepsy, characterized in that: The present invention also provides a method for producing a lipid peroxide inhibitor for epilepsy comprising a water and / or organic solvent extract of Hakutsuru Reishi as a main component,
A method for producing a lipid peroxide inhibitor for epilepsy, comprising extracting Hakutsuru Reishi with ethanol. The present invention also relates to a method for producing a lipid peroxide inhibitor of epilepsy comprising a water and / or organic solvent extract of Hakutsuru reishi as a main component, comprising the steps of: A method for producing a lipid peroxide inhibitor for epilepsy, characterized by extracting with water. Also, the present invention
A method for producing a lipid peroxide inhibitor for epilepsy based on a water and / or organic solvent extract of Hakutsuru Reishi, comprising roasting sun-dried and / or drum-dried Hakutsuru Reishi. A method for producing a lipid peroxide inhibitor for epilepsy, comprising extracting the roasted Hakutsuru Reishi with water and / or an organic solvent. Also, the present invention
A method for producing a lipid peroxide inhibitor for epilepsy comprising a water and / or organic solvent extract of Hakutsuru Reishi as a main component, wherein Hakutsuru Reishi is pulverized to 12 mesh or less using a pulverizer. A method for producing a lipid peroxide inhibitor for epilepsy, comprising extracting the extracted Hakutsuru Reishi with water and / or an organic solvent.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION As the white cranes used in the present invention, commercially available leaves, whole plants (aboveground parts) or a mixture of these with roots may be used. Preferably, these are sun-dried and / or drum-dried and pulverized with a pulverizer to 20 mesh or less, preferably 12 mesh or less. If necessary, this Hakutsuru Reishi may be obtained by sun-dried and / or drum-dried and roasted by a roaster.

[0007] The thus obtained Hakutsuru Reishi is extracted with water and / or an organic solvent to obtain an extract which is the lipid peroxide inhibitor of the present invention.

In the present invention, the water used for the extraction includes cooling water, room temperature water and hot water.

In the present invention, the organic solvent used for extraction includes ethanol, methanol, propanol,
Alcohols such as 1,3-butylene glycol, ethers such as dimethyl ether, diethyl ether and methyl ethyl ether, ketones such as acetone, phenols such as benzene, hexane, heptane, liquid paraffin,
Examples thereof include hydrocarbons such as squalane and the like, and these can be used alone or as a mixture of two or more.

The resulting water and / or organic solvent extract of Hakutsuru Reishi is a clear, green-brown to deep green liquid having an odorless or slight fragrance and a unique rich taste. Although it may be purified, it is preferably purified by filtration, centrifugation or other methods, and further suitably concentrated and used by means such as distillation. Further, this extract may be used as a transparent green-brown to deep green fine powder having an odorless or slight aroma and a unique rich taste obtained by further treatment by distillation to dryness, freeze-drying or the like. It is possible.

Next, the present invention will be described with reference to examples and test examples, but these do not limit the technical scope of the present invention.

Example 1 Sun-dried and then drum-dried Hakutsuru Reishi whole plant (sold by Daikyo Bussan Co., Ltd .; hereafter the same place for purchasing Hakutsuru Reishi) 1k
g is crushed by a crusher, roasted in a roaster, and further crushed, and the obtained crushed material is sieved and classified to 12 mesh or less to obtain about 0.8 kg of a ground crushed white crane Reishi. Was. 2.5 g of the ground white cranes of Reishi was immersed in 100 ml of hot water and filtered to obtain a green-brown transparent liquid extract with a slight jasmine-like aroma and a unique rich taste.

Example 2 2.5 g of ground grass of Hakutsuru Reishi was immersed in 100 ml of water.
Filtration yielded a deep green, clear liquid extract with a slight aroma and a unique rich taste.

Example 3 2.5 g of ground leaves of white crane Reishi was immersed in 100 ml of ethanol and filtered to obtain a deep green transparent liquid extract with a slight aroma and a unique rich taste. .

Example 4 1.3 g of a crushed leaf, stem and root of Hakutsuru Reishi
It was immersed in 100 ml of butylene glycol and filtered to obtain a deep green transparent liquid extract with a slight aroma and a unique rich taste.

Example 5 3 g of ground leaves and roots of Hakutsuru Reishi were immersed in 100 ml of 1: 1 ethanol water, filtered to give a deep green transparent liquid with a slight aroma and a unique rich taste. An extract was obtained.

Example 6 2.5 g of ground crushed white crane leaf was immersed in 100 ml of hot water and filtered to obtain a green-brown transparent liquid extract having a slight aroma and a unique rich taste. .

Example 7 Example 3 except that acetone was used instead of ethanol.
To give a deep green transparent liquid extract with a slight aroma and a unique rich taste.

Example 8 Example 3 except that ether is used instead of ethanol
To give a deep green transparent liquid extract with a slight aroma and a unique rich taste.

Example 9 Example 3 except that benzene was used instead of ethanol
To give a deep green transparent liquid extract with a slight aroma and a unique rich taste.

Example 10 Sun-dried and then drum-dried whole grass and roots of Hakutsuru Reishi were crushed by a crusher, roasted by a roaster, and further crushed. It is sieved and classified to 12 mesh or less.
Except to obtain g, treat in the same manner as in Example 1 to give a slight aroma,
A green-brown transparent liquid extract with a unique rich taste was obtained.

Example 11 1000 ml of extract obtained by treating in the same manner as in Example 1
Was freeze-dried to give about 10 mg of a greenish brown fine powder with a slight aroma and a unique rich taste.

Example 12 1000 ml of extract obtained by treating in the same manner as in Example 2
Was freeze-dried to give about 10 mg of a greenish brown fine powder with a slight aroma and a unique rich taste.

Example 13 1000 ml of extract obtained by treating in the same manner as in Example 3
Was lyophilized to give about 10 mg of a deep green fine powder with a slight aroma and a unique rich taste.

Example 14 1000 ml of extract obtained by treating in the same manner as in Example 4
Was lyophilized to give about 10 mg of a deep green fine powder with a slight aroma and a unique rich taste.

Test Example A rat of a traumatic epilepsy model was prepared by injecting an iron salt into the cerebral cortex of a rat, and a dextrin doubled product of a hot water extract of the whole plant of Hakutsuru Reishi was extracted by the method of Example 1. Was administered to examine the inhibitory effect of lipid peroxide. The inhibitory effect of lipid peroxide is due to the use of Malonalde, a metabolite of lipid peroxide.
The determination was made by measuring the hydraulic (MD @). Test method 1) Administered sample Dextrin doubled product of Hakutsuru Reishi whole plant hot water extract (1:
1) (Maruzen Pharmaceutical sales) Dextrin (Wako Pure Chemicals sales) 2) Administration method Dextrin double-drinked product of hot water extract of Hakutsuru Reishi whole plant is 8m
g / ml, and dextrin was adjusted to 4 mg / ml with tap water, and each was freely ingested. Controls received tap water ad libitum. 3) Administration period Preparation of model rats 20 days before iron salt injection and 10 days after iron salt injection Model rats were prepared by injecting iron salts into Wistar rats (5 to 8 rats per group). That is, 0.1 m of the left cerebral cortex of a rat anesthetized with ether was injected with a microsyringe.
5 μl of ol / l FeCl 3 was injected. The control is Fe
5 physiological saline adjusted to pH 1.7 instead of Cl3
μl was injected. In addition, as a comparative group, an epilepsy model rat group administered only with water and an epilepsy model rat group administered only with a sample base without administration of the Hakutsuru Reishi dextrin double powder according to the present invention. After suturing the wound, the animals were bred for 10 days while the administration was continued. 4) MDA measurement The cerebral cortex of the model rat was tripled to 20 mmol / l
The supernatant was homogenized in a Tris-HCl (pH 7.4) buffer, and centrifuged at 3,000 × g at 4 ° C. for 10 minutes. OXIS Inter for measurement
National's Bioxytec LPO-58
6 was used. At the same time, the amount of protein for correction was also measured. Table 1 shows the results and discussion results. The MDA value of the group administered with water / iron salt (epilepsy model rat) was significantly increased as compared with the group administered with water / saline (control) (P <0.01). The MDA value was significantly reduced in the sample / iron salt-administered (Hakutsuru Reishi dextrin double powdered) group compared to the water / iron salt-administered (epilepsy model rat) group (P <0.00).
5) The values were almost the same as the control. The MDA was increased in the water / iron salt administration (dextrin) group as compared to the control, and it was recognized that dextrin had no inhibitory effect on lipid peroxide. On the other hand, in the sample / iron salt administration (Hakutsuru Reishi dextrin double powder) group, the MDA value increased by injecting the iron salt was reduced, and the lipid peroxide was added to the sample base (dextrin). Since there was no inhibitory effect, this effect was recognized to be due to Hakutsuru Reishi.

[0026]

Industrial Applicability The lipid peroxide inhibitor of epilepsy of the present invention has an excellent action of suppressing the production of lipid peroxide in the cerebral cortex derived from traumatic epilepsy, and the disruption of the membrane structure by lipid peroxide. It can be expected to prevent widespread membrane damage and secondary cytotoxicity. Further, the lipid peroxide inhibitor of epilepsy of the present invention comprises natural water and / or
Or, since it is an organic solvent extract, it is highly safe and has the advantage of not causing harmful effects on the human body even if it is used for a long time. According to the method for producing a lipid peroxide inhibitor of epilepsy of the present invention, such a lipid peroxide inhibitor of epilepsy can be obtained.

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Claims (8)

(57) [Claims] 1. A lipid peroxide inhibitor for epilepsy, characterized by containing water and / or an organic solvent extract of Hakutsuru Reishi as a main component. 2. A lipid peroxide inhibitor for epilepsy, characterized by comprising a water and / or organic solvent extract of Hakutsuru Reishi as a main component. 3. A lipid peroxide inhibitor for epilepsy, comprising a fine powder obtained by treating a water and / or organic solvent extract of Hakutsuru Reishi as a main component. 4. A method for producing a lipid peroxide inhibitor for epilepsy according to claim 1, comprising extracting Hakutsuru reishi with hot water. Method of manufacturing the agent. 5. A method for producing a lipid peroxide inhibitor of epilepsy according to claim 1, wherein the epilepsy is extracted with ethanol. Manufacturing method. 6. A method for producing a lipid peroxide inhibitor for epilepsy according to claim 1, comprising the steps of:
A method for producing a lipid peroxide inhibitor for epilepsy, characterized by extracting with "1: 1 aqueous ethanol". 7. A method for producing a lipid peroxide inhibitor for epilepsy according to claim 1, wherein the sun-dried and / or drum-dried Hakutsuru Reishi is roasted and the roasted. A method for producing a lipid peroxide inhibitor for epilepsy, comprising extracting Hakutsuru Reishi with water and / or an organic solvent. 8. A method for producing the lipid peroxide inhibitor for epilepsy according to claim 1, wherein the white cranes are ground with a crusher to 12 mesh or less. A method for producing a lipid peroxide inhibitor for epilepsy, comprising extracting with water and / or an organic solvent.