JP3328959B2 - centrifuge - Google Patents
centrifugeInfo
- Publication number
- JP3328959B2 JP3328959B2 JP19819892A JP19819892A JP3328959B2 JP 3328959 B2 JP3328959 B2 JP 3328959B2 JP 19819892 A JP19819892 A JP 19819892A JP 19819892 A JP19819892 A JP 19819892A JP 3328959 B2 JP3328959 B2 JP 3328959B2
- Authority
- JP
- Japan
- Prior art keywords
- rotor
- projection
- stationary
- door
- rotating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Centrifugal Separators (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、遠心力と流れの力を利
用して細胞を大きさ別に分離するエルトリエータロータ
を備えた遠心分離機に関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a centrifuge provided with an elutriator rotor for separating cells by size using centrifugal force and flow force.
【０００２】[0002]
【従来の技術】図３、図４に従来におけるエルトリエー
タロータのシール部固定機構を示す。エルトリエータロ
ータは、回転中に液体を連続的に導入して分離された細
胞を回収する機構部分がある。この機構部は面シール方
式を採用しており回転部２と静止部１に分かれていて回
転部２はロータ部６に、静止部１は遠心分離機のチャン
バ７に固定されている。静止部１の固定方法は円筒形を
しているチャンバ７の内壁にリング状の固定バンド８を
バンド直径調節ネジ１１で固定バンド８の直径を段々大
きくしていって突っ張ることでチャンバ７に固定し、こ
の固定バンド８から固定アーム９を出し、固定ネジ１０
で静止部１に固定する方法である。2. Description of the Related Art FIGS. 3 and 4 show a conventional fixing mechanism for a seal portion of an elutator rotor. The elutator rotor has a mechanism for continuously introducing a liquid during rotation to collect the separated cells. This mechanism employs a face seal system, and is divided into a rotating unit 2 and a stationary unit 1. The rotating unit 2 is fixed to the rotor unit 6, and the stationary unit 1 is fixed to the chamber 7 of the centrifuge. The stationary part 1 is fixed to the chamber 7 by gradually increasing the diameter of the fixed band 8 with the band diameter adjusting screw 11 on the inner wall of the cylindrical chamber 7 and tightening it. Then, the fixing arm 9 is taken out from the fixing band 8, and the fixing screw 10 is fixed.
This is a method of fixing to the stationary unit 1 with.
【０００３】[0003]
【発明が解決しようとする課題】従来の固定方法はバン
ドを固定する位置が限定されているので数回の調整が必
要な上、取付けずらく準備に大変手間がかかるものであ
った。本発明の目的はシール部の静止部を自動的に固定
し準備にかかわるわずらわしさを排除するものである。In the conventional fixing method, the position at which the band is fixed is limited, so that several adjustments are required, and the mounting is difficult and the preparation is very troublesome. SUMMARY OF THE INVENTION It is an object of the present invention to automatically fix a stationary portion of a seal portion to eliminate troublesome preparation.
【０００４】[0004]
【課題を解決するための手段】上記目的は、シール部の
静止部の上面より上方に位置するドアに向い突起部を出
すとともにドアからもシール部へ向け突起物をだし、静
止部がロータとともに回転しだすと両突起が接触するよ
うに位置させることで達成される。SUMMARY OF THE INVENTION The object of the present invention is to provide a projection protruding toward a door located above an upper surface of a stationary portion of a seal portion, and projecting a projection from the door toward the seal portion. This is achieved by positioning the projections so that they contact each other when the rotation starts.
【作用】上部にシール部（回転部、静止部ともに含む）
を取付けたエルトリエータロータを遠心分離機のチャン
バ内にある回転シャフトにセットする。この時の静止部
にある突起部とドアに付いている突起物が、ドアを閉め
た時に当らないように位置させる。この状態よりドアを
閉めロータを回転させると、当初は静止部とロータ部が
一緒に回転しだすが一回転する間には、ドアから出てい
る突起物と静止部から出ている突起部が接触し静止部は
それ以上回転ができなくなる。このようにして静止部を
固定することができる。[Function] Sealing part on top (including both rotating and stationary parts)
Is set on the rotating shaft in the chamber of the centrifuge. At this time, the projection on the stationary part and the projection on the door are positioned so as not to hit when the door is closed. When the door is closed and the rotor is rotated from this state, the stationary part and the rotor part start rotating together at first, but during one rotation, the projection coming out of the door and the projection coming out of the stationary part come into contact. However, the stationary part cannot rotate any more. In this way, the stationary part can be fixed.
【０００５】[0005]
【実施例】本実施例におけるエルトリエータロータ（以
下ロータと称す）６を備えた遠心分離機のシール部固定
機構を図１及び図２を用いて説明する。図１は本実施例
におけるシール部固定機構を示す一部縦断側面図、図２
は図１のＡ−Ａ線断面図である。図１及び図２に示す遠
心分離機は、駆動モータと、この駆動モータによって回
転されるロータ６の上部に位置する回転部２と、この回
転部２と相対回転可能に配され且つ回転部２と共回り
（つれ回り）してしまう静止部１と、この静止部１及び
回転部２から成るシール部と、ロータ６を収容するため
のチャンバ７と、このチャンバ７を閉塞可能なドア３と
を有している。 DESCRIPTION OF THE PREFERRED EMBODIMENTS The elutator rotor according to this embodiment
Fixing the seal of the centrifuge equipped with 6)
The mechanism will be described with reference to FIGS. FIG. 1 shows the present embodiment.
FIG. 2 is a partially longitudinal side view showing a seal portion fixing mechanism in FIG.
FIG. 2 is a sectional view taken along line AA of FIG. 1. 1 and 2 shown in FIG.
The core separator is driven by the drive motor and the drive motor.
The rotating part 2 located above the rotor 6 to be rotated,
It is arranged so as to be relatively rotatable with the rotating part 2 and rotates together with the rotating part 2
The stationary part 1 (which rotates) and the stationary part 1 and
For accommodating the seal part composed of the rotating part 2 and the rotor 6
And a door 3 capable of closing the chamber 7
have.
【０００６】また、静止部１の上面には、ドア３側に向
い突出する突起部４が設けられており、この突起部４と
回転接触可能な突起物５がドア３の底面に設けられてい
る。なお、上記突起部４は、静止部１の回転中心軸（ロ
ータ６の回転中心軸と同軸） からズレた静止部１の上面
に設けられており、また突起物５は上記静止部１と回転
接触するために、静止部１の回転中心軸から同量ズレた
ほぼ同一半径上に位置するドア３の底部に設けられてい
る。 [0006] On the upper surface of the stationary portion 1, the door 3 side is provided.
A protruding portion 4 is provided.
A protrusion 5 that can rotate and contact is provided on the bottom surface of the door 3.
You. In addition, the above-mentioned protrusion 4 is provided with a rotation center axis (b
Upper surface of the stationary part 1 shifted from the rotation center axis of the motor 6)
And the protrusion 5 rotates with the stationary part 1.
Due to contact, it is shifted by the same amount from the rotation center axis of the stationary part 1.
It is provided at the bottom of the door 3 which is located on almost the same radius.
You.
【０００７】更に遠心力と液体（試料）の流れる力を利
用して細胞等を大きさ別に分離するロータ６内に液体を
流入するための導入パイプ１２と、分離された細胞等を
流出するための回収パイプ１３とを有しており、これら
導入パイプ１２及び回収パイプ１３は、静止部１内に挿
通して設けられている。なお、静止部１及び回転部２間
の摩擦抵抗に伴い複数回以上、静止部１が回転部２と共
回りしてしまうと、導入パイプ１２及び回収パイプ１３
が捩れてしまい、両パイプ１２，１３の流路が遮断され
液体の導入或いは回収に支障をきたすなどの不具合が起
きてしまう。そこで、このような不具合を解消するため
に本実施例のシール部は、共回りに伴い静止部１が回転
したとしても回転角３６０°以下のどこかで突起部４と
突起物５とが回転接触するように構成しているため、静
止部１が遊転するので、上述したように導入パイプ１２
及び回収パイプ１３の捩れ具合を全く問題のない範囲
（許容範囲）に抑えることができる。 Furthermore, the centrifugal force and the flowing force of the liquid (sample) are used.
Liquid in the rotor 6 to separate cells etc. by size using
The introduction pipe 12 for inflow and the separated cells etc.
And a recovery pipe 13 for flowing out.
The introduction pipe 12 and the recovery pipe 13 are inserted into the stationary unit 1.
It is provided through. In addition, between the stationary part 1 and the rotating part 2
More than once due to the frictional resistance of the
If it turns, the introduction pipe 12 and the recovery pipe 13
Is twisted, and the flow paths of both pipes 12 and 13 are blocked.
Failures such as disturbing the introduction or recovery of liquid
Will come. Therefore, in order to solve such a problem
In the seal portion of this embodiment, the stationary portion 1 rotates with the co-rotation.
Even if it does, the protruding part 4
Since the projection 5 is configured to be in rotational contact, the static
Since the stop 1 idles, the introduction pipe 12
And the degree of twisting of the recovery pipe 13 is within a range without any problem.
(Allowable range).
【０００８】次にロータ６による遠心分離について説明
する。上述した駆動モータによりロータ６を回転する
と、当該ロータ６の上部に位置する回転部２も共に回転
する。回転部２が回転すると回転部２と相対回転可能に
配されている静止部１がドア３の底面に設けた突起物５
に静止部１に設けた突起部４が回転接触する位置まで共
回りする。突起部４と突起物５とが回転接触するとロー
タ６と共に回転部２が回転するものの静止部１は回転接
触位置で回転方向動作を規制されるため、導入パイプ１
２及び回収パイプ１３の捩れを抑制しながら導入パイプ
１２内の液体を確実にロータ６内に供給することができ
る。供給された液体は遠心分離され、その後、分離液は
試料の流れに応じて回収パイプ１３内を通り取り出され
る。 Next, centrifugal separation by the rotor 6 will be described.
I do. The rotor 6 is rotated by the drive motor described above.
And the rotating part 2 located above the rotor 6 also rotates.
I do. When the rotating part 2 rotates, it can rotate relative to the rotating part 2
The protrusion 5 provided on the bottom surface of the door 3 by the stationary portion 1
To the position where the protrusion 4 provided on the stationary part 1
Go around. When the projection 4 and the projection 5 come into rotational contact, a low
The rotating part 2 rotates together with the rotor 6, but the stationary part 1
Since the rotation direction operation is restricted at the contact position, the introduction pipe 1
2 and introduction pipe while suppressing torsion of recovery pipe 13
12 can be reliably supplied into the rotor 6
You. The supplied liquid is centrifuged, and then the separated liquid is
It is taken out through the collection pipe 13 according to the flow of the sample.
You.
【０００９】このように回転されるロータ６内に液体を
供給し、そして分離液を回収する一連の動作を実行する
場合には、必ず回転部２と共回りする静止部１の回転を
突起 物５と突起部４とが回転接触する位置で自動的に止
められるため、静止部１の取付けに手間をかける必要が
なくなり組立作業の向上を図ることができる。 [0009] The liquid is supplied into the rotor 6 rotated in this manner.
Supply and perform a series of operations to recover the separated liquid
In this case, the rotation of the stationary unit 1
Automatically stop at a position where the protrusion 5 and the protruding portions 4 is rotated in contact
It is necessary to take time to attach the stationary part 1
Thus, the assembling work can be improved.
【００１０】[0010]
【発明の効果】本発明によればシール部の固定にかかわ
る作業が不要となり自動的に固定でき、準備にかかわる
わずらわしさを排除する。According to the present invention, the operation for fixing the seal portion is not required, and the seal can be automatically fixed, thereby eliminating the troublesome work involved in the preparation.
【図１】 図１は本発明のシール部固定機構の側面断面
図である。FIG. 1 is a side sectional view of a seal portion fixing mechanism of the present invention.
【図２】 図２は本発明のシール部固定機構のＡ−Ａ断
面図である。FIG. 2 is a sectional view taken along line AA of the seal portion fixing mechanism of the present invention.
【図３】 図３は従来のシール部固定機構の側面断面図
である。FIG. 3 is a side sectional view of a conventional seal portion fixing mechanism.
【図４】 図４は従来のシール部固定機構のＢ−Ｂ断面
図である。FIG. 4 is a cross-sectional view of the conventional sealing portion fixing mechanism taken along line BB.
１はシール部の静止部、２はシール部の回転部、３はド
ア、４は静止部の突起部、５はドアの突起物、６はエル
トリエータロータ、７は遠心分離機のチャンバ、８は固
定バンド、９は固定アーム、１０は固定ネジ、１１はバ
ンド直径調節ネジ、１２は導入パイプ、１３は回収パイ
プである。1 is a stationary portion of the sealing portion, 2 is a rotating portion of the sealing portion, 3 is a door, 4 is a projection of the stationary portion, 5 is a projection of the door, 6 is an elbow.
A triator rotor , 7 is a centrifuge chamber, 8 is a fixed band, 9 is a fixed arm, 10 is a fixed screw, 11 is a band diameter adjusting screw, 12 is an introduction pipe, and 13 is a recovery pipe.
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 昭57−174166（ＪＰ，Ａ) 特開 昭62−234559（ＪＰ，Ａ) 特開 昭61−174957（ＪＰ，Ａ) 実開 昭60−83065（ＪＰ，Ｕ) 実開 昭60−83066（ＪＰ，Ｕ) 実開 昭60−83068（ＪＰ，Ｕ) 特公 昭39−18685（ＪＰ，Ｂ１) (58)調査した分野(Int.Cl.7，ＤＢ名) B04B 5/00 C12M 1/00 ──────────────────────────────────────────────────続 き Continuation of front page (56) References JP-A-57-174166 (JP, A) JP-A-62-234559 (JP, A) JP-A-61-174957 (JP, A) 83065 (JP, U) JP-A 60-83066 (JP, U) JP-A 60-83068 (JP, U) JP-B 39-18685 (JP, B1) (58) Fields investigated (Int. Cl. 7 , DB name) B04B 5/00 C12M 1/00
Claims (1)
ロータに一体回転可能に設けられる回転部と、該回転部
と共にシール部を構成する静止部と、前記ロータを収容
するチャンバと、該チャンバを閉塞可能なドアとを備え
た遠心分離機において、前記回転部と相対回転可能な前
記静止部に突起部を設け、該突起部と回転接触可能な突
起物を前記ドアに設け、該突起物に前記突起部が回転接
触することで前記静止部の回転方向動作を規制するよう
に構成することを特徴とする遠心分離機。1. A rotor into which a liquid is continuously introduced , and
A rotating part provided on the rotor to be integrally rotatable, and the rotating part
Accommodating a stationary part which forms a seal part together with the rotor
And a door capable of closing the chamber.
Centrifugal separator before rotating relative to the rotating part.
A projection is provided on the stationary part, and a projection that can be brought into rotational contact with the projection is provided.
An origin is provided on the door, and the projection is in rotational contact with the projection.
By touching it, it regulates the rotation direction operation of the stationary part.
Centrifuge characterized that you configuration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19819892A JP3328959B2 (en) | 1992-07-24 | 1992-07-24 | centrifuge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP19819892A JP3328959B2 (en) | 1992-07-24 | 1992-07-24 | centrifuge |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0639315A JPH0639315A (en) | 1994-02-15 |
| JP3328959B2 true JP3328959B2 (en) | 2002-09-30 |
Family
ID=16387109
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP19819892A Expired - Fee Related JP3328959B2 (en) | 1992-07-24 | 1992-07-24 | centrifuge |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3328959B2 (en) |
-
1992
- 1992-07-24 JP JP19819892A patent/JP3328959B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0639315A (en) | 1994-02-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20020618 |
|
| LAPS | Cancellation because of no payment of annual fees |