JP3346083B2 - Antibacterial agent and oral composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antibacterial agent against caries and periodontal disease-causing bacteria and an oral composition containing the same.

[0002]

2. Description of the Related Art
Chlorhexidine, cetylpyridinium chloride, and the like have been used as oral fungicides, but there are cases in which the effects on the flora may be concerned.

[0003] In view of these points, highly safe antibacterial agents are required, and various studies have been made on using plant extracts as active ingredients in oral products. For example, as an antibacterial agent against caries and periodontal disease-causing bacteria,
No. 169413, Murasaki, JP-A-61-26
In Japanese Patent Publication No. 0012, fennel is disclosed in JP-A-62-2050.
In JP-A-16-163, Oubak, Ouren, Orgon, Keihi, Kouboku, Gennoshoko, Inchinko, and JP-A-4-364115, Kumaseba, JP-A-5-23
No. 153, JP-A-5-163128, Ketsumeishi, Shazenshi, JP-A-5-2018
No. 43 proposes a birch extract or the like, but none of them has a satisfactory activity.

The present invention has been made in view of the above circumstances, and has as its object to provide an antibacterial agent having excellent antibacterial activity against caries and periodontal disease-causing bacteria, and an oral composition containing the same. And

[0005]

Means for Solving the Problems and Actions The present inventors have conducted intensive studies to achieve the above-mentioned object. As a result, the extract of the plant of the genus Muraya used as a spice is Streptococcus sp. Mutans (Stre
ptococcus mutans and polyphyromonas gingivalis (Porphy)
and high antibacterial activity against cariogenic and periodontal disease-causing bacteria is due to mahanin, isomahanin, and murayanol contained in extracts of plants of the genus Muraya. The inventor has found that the present invention has been accomplished.

Accordingly, the present invention provides an antibacterial agent against caries and periodontal disease-causing bacteria, comprising an extract of a plant of the genus Muraya, or one or more selected from mahanin, isomahanin and murayanol as active ingredients. An oral composition comprising an antibacterial agent is provided.

[0007] below, and will be described in further per the present invention in detail, Murraya plants of the genus to be used in the present invention belongs to the citrus family, south India, the Himalayas, native to northern Myanmar curry tree (Murraya K oenigii
Spreng. ) And native geckets (M. exotica L., M. panicul) in North India and Taiwan
ata (L.) Jack) and the like are known. The leaf of P. japonicus has long been called curry leaf in India, and has been used like a laurel as a spice. It is also used as a stomachic and tonic.

In the present invention, as an antibacterial agent against Streptococcus mutans, a cariogenic bacterium, and polyphyromonas gingivalis, a periodontal causative bacterium, the extract of the plant of the genus Muraya or mahanin contained in the extract is used. , Isomahanin and mullanol.

[0009] When an extract of the plant of the genus Muraya is obtained, any part of the plant of the genus Muraya including mahanin, isomahanin or murayanol, such as leaves, fruits, stems and rhizomes, can be used. Further, as the extraction solvent, water, an acid, an alkali or an aqueous solution of a salt thereof,
Furthermore, ethyl ether, ethylene chloride, dioxane, acetone, ethanol, methanol, ethyl acetate, propylene glycol, n-hexane, petroleum ether, ligroin, cyclohexane, carbon tetrachloride, chloroform, dichloromethane, 1,2-dichloroethane, toluene, benzene, etc. Or a mixed solvent in which two or more of these solvents are mixed, and a polar solvent is particularly preferable. The extraction can be performed according to a conventional method. The plant of the genus Muraya is immersed in an extraction solvent at room temperature or under heating, and the extract is collected. The extract and the extraction residue may be subjected to the above-mentioned extraction treatment again as needed to obtain an extract. In this case, a solvent different from the above solvent can be used as the extraction solvent. Thereafter, the solvent can be distilled off from the extract to obtain an extract. In this case, if the extraction solvent is a non-toxic one such as water, ethanol, or water-ethanol, the extract can be used as it is. Before or after obtaining the extract, essential oils can be removed by steam distillation for deodorization, and adsorbent treatment can be performed for decolorization.

[0010] Mahanin, isomahanin and mullanol can be isolated from the above extract by a method such as silica gel column chromatography, or may be produced by synthesis.

[0011] The antibacterial agent comprising the extract of the plant of the genus Muraya or the antibacterial agent comprising one or more of mahanin, isomahanin and murayanol may be used in dentifrices such as toothpaste and liquid dentifrice, pasta for oral cavity, mouthwash. It can be used by blending it with an oral composition such as a mouthwash.

In this case, the compounding amount of the above antibacterial agent is an effective amount of the antibacterial agent, and is usually 0.0001 to 5 in the oral composition.
% (% By weight, hereinafter the same), particularly 0.001 to 3%.

Other components of the oral composition of the present invention include:
Known components according to the type can be appropriately selected and used, and the blending amount can be a normal dose. For example, toothpaste can be prepared using ingredients such as an abrasive, a thickener, a binder, a surfactant, a sweetener, and a flavor.

[0014]

Industrial Applicability The antibacterial agent of the present invention and an oral composition using the same have high antibacterial activity against Streptococcus mutans, a cariogenic bacterium, and polyphyromonas gingivalis, a periodontal causative bacterium. Therefore, it has a good preventive effect against caries and periodontal disease, and has high safety in use, because the plants of the genus Muraya are used as spices.

[0015]

EXAMPLES Hereinafter, the present invention will be described in detail with reference to experimental examples and examples, but the present invention is not limited to the following examples.

[Experimental Example] To a test tube (13 × 100 mm) containing 4 ml of the following liquid medium, 0.04 ml of an antibacterial component solution having a stepwise-concentrated concentration was added and mixed. 04 ml was added and stirred. This was anaerobically cultured at 37 ° C. for 3 days, and the growth amount of the bacteria was measured by the absorbance at 550 nm. An absorbance value of less than 0.05 was regarded as effective, and the minimum growth inhibitory concentration of each antibacterial component was determined. Table 1 shows the results. Bacterial strain used and composition of liquid medium Polyphyromonas gingivalis 381 (Pg) Medium: Todd Hewitt broth (THB) 3% by weight Hemin 3 ppm Menadione 0.5 ppm Streptococcus mutans 6715 (Sm) Medium: THB 3% by weight

[0017]

[Table 1]

From the results shown in Table 1, the extract of the plant of the genus Muraya and mahanin, isomahanin and murayanol were superior to both the periodontal disease-causing bacteria Polyphyromonas gingivalis and the cariogenic bacteria Streptococcus mutans. It is recognized to exhibit antibacterial properties.

Next, an embodiment will be described. [Example 1] Toothpaste calcium carbonate 50.00% glycerin 20.00 carrageenan 0.50 carboxymethylcellulose 1.00 lauryl diethanolamide 1.00 sucrose monolaurate 2.00 fragrance 1.00 saccharin 0.10 Leaf ethanol extract 0.20 Water residue 残 100.00

Example 2 Toothpaste Dibasic calcium phosphate dihydrate 50.00% Glycerin 20.00 Carboxymethylcellulose 2.00 Sodium lauryl sulfate 2.00 Fragrance 1.00 Saccharin sodium 0.10 50% of geckotsu leaves Ethanol extract 0.50 Water residue ──────────────────────────────── 100.00

Example 3 Toothpaste Aluminum hydroxide 45.00% Gel silica 2.00 Sorbit 25.00 Carboxymethylcellulose 1.00 Sodium lauryl sulfate 2.00 Fragrance 1.00 Saccharin sodium 0.10 Weakly acidic water extract 0.20 Water remaining １０ 100.00

Example 4 Toothpaste Precipitated silica 25.00% Glycerin 25.00 Sorbit 25.00 Lauroyl polyglycerin ester 1.00 Polyoxyethylene sorbitan monolaurate 2.00 Fragrance 1.00 Saccharin sodium 0.20 Isomahanin 0.05 Water residue １０ 100.00

Example 5 Liquid Toothpaste Aluminum Hydroxide 25.00% Glycerin 40.00 Sorbit 15.00 Carboxymethyl Cellulose 0.20 Propylene Glycol 2.00 Sodium Lauryl Sulfate 1.50 Decaglyceryl Monolaurate 1.00 Fragrance 1 5.00 Saccharin sodium 0.10 Ethanol extract of Pterodactylus leaf 0.10 Water balance １００ 100 .00

Example 6 Liquid Toothpaste Precipitable Silica 20.00% Glycerin 25.00 Sorbit 35.00 Xantham Gum 0.20 Propylene Glycol 2.00 Sodium Lauryl Sulfate 1.50 Decaglyceryl Monolaurate 2.00 Fragrance 1. 00 Saccharin sodium 0.10 Murayanol 0.30 Water remaining 100.00

Example 7 Oral Pasta Liquid Paraffin 15.00% Cetanol 10.00 Glycerin 20.00 Polyoxyethylene Sorbitan Fatty Acid Ester 5.00 Fragrance 0.50 Saccharin Sodium 0.10 Geketsu Leaves Ethanol Extract 0.50 Water remaining ────────────────────────────────100.00

Example 8 Mouthwash Ethanol 20.00% Fragrance 1.00 Polyoxyethylene hydrogenated castor oil 0.30 Sodium monofluorophosphate 0.10 Saccharin sodium 0.05 Steam distillation residue of gecko leaf hexane extract 0 .20 water remaining １０100.00

Example 9 Gargle Tablets Sodium Bicarbonate 54.00% Citric Acid 17.00 Anhydrous Sodium Sulfate 12.80 Dibasic Sodium Phosphate 10.00 Polyethylene Glycol 3.00 Sodium Monofluorophosphate 0.10 Fragrance 2.00 Oleic acid 0.10 Acetone extract of Pseudomonaceae leaves 1.00 ──────────────────────────────── 100.00

Continuation of the front page (51) Int.Cl. ⁷ Identification code FI A61P 31/04 A61P 31/04 (56) References JP-A-8-225420 (JP, A) JP-A-4-279517 (JP, A) JP-A-4-346933 (JP, A) JP-A-7-82133 (JP, A) JP-A-8-175941 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 7/00-7/50 A61K 35/78-35/84 JICST file (JOIS) WPI (DIALOG)

Claims (4)

(57) [Claims] 1. An antibacterial agent against caries and periodontal disease-causing bacteria, comprising an extract of a plant of the genus Muraya as an active ingredient. 2. An antibacterial agent against caries and periodontal disease-causing bacteria, comprising one or more active ingredients selected from mahanin, isomahanin and mullanol. 3. An oral composition comprising an extract of the genus Muraya. 4. An oral composition comprising one or more selected from mahanin, isomahanin and mullanol.