JP3385293B2 - Altocarpine-containing antibacterial and preservatives and cosmetics - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a novel antibacterial / preservative containing altocarpine and its application to cosmetics.

It can be used as various cosmetics in the fields of pharmaceuticals, quasi-drugs, and cosmetics (including various preparations used for humans and other animals), and specifically, lotions and lotions. , Shaving lotion, emulsion, cream (including ointment), sunscreen, foundation, oil, pack, soap (including medicated soap), body soap, nail cosmetics, eyebrow cosmetics, perfume, face wash, oral cavity Applications include applications (toothpaste, mouthwash, etc.), liquid odors / deodorants, bath agents, shampoos, conditioners, hair tonics, hair sprays, hair dyes, etc. As antibacterial and antiseptic agents, various forms of oral agents, textiles (sheets, clothes, etc.), non-woven fabrics such as sanitary cotton, wet tissues and wipes, disinfectant cloth, etc. Composition (gum, candy, etc.), fish paste products such as kamaboko and chikuwa, livestock products such as sausage and ham, western confectionery, Japanese confectionery, raw noodles, Chinese noodles, boiled noodles, noodles such as buckwheat, sauces,
It can be used for general food and drink such as soy sauce, seasonings such as sauces, pickles, soybeans, etc.

[0003]

2. Description of the Related Art So far, sorbic acid has been used as an antibacterial / preservative agent for foods, pharmaceuticals, cosmetics, etc. due to the growth of microorganisms.
Dehydroacetic acid and its salts, paraoxybenzoic acid derivatives and the like are often used, but there is a problem in safety,
Addition amount and target foods are limited (for example, the upper limit of compounding sorbic acid and its salts in foods is 0.2%, dehydroacetic acid and its salts can be used only in cheese, butter and margarine, and paraoxybenzoic The upper limit of the content of sodium methyl acidate in cosmetics was 1.0%).

In order to suppress the growth of microorganisms, the invasion of microorganisms is suppressed by a chemical method such as physical distribution in the skin or mucous membranes of living organisms or the distribution of bactericidal substances or mucus. Resistance to microorganisms is caused by innate and nonspecific infection resistance, immune response to specific pathogenic bacteria, such as adhesion inhibition by immunoglobulin, phagocytosis by phagocytic cells, lytic action by lysozyme, and the like.

However, if the infectivity of bacteria exceeds the resistance in the body, infectious symptoms appear in the body, for example, infectious dermatitis such as pyoderma due to Staphylococcus aureus, food poisoning, sepsis, Bacillus subtilis is conjunctivitis, iritis, Escherichia coli is urinary tract infection, diarrhea, etc., periodontal disease due to Actinobacillus, cariogenic dental caries, and acne bacterium (Propionibacterium acnes), dandruff There is a risk of causing various symptoms such as acne due to (Pitirosporum ovale), dandruff or itching due to dandruff.

[0006] Particularly during adolescence, acne and dandruff often occur, and the name of acne is called acne vulgaris. Clinically, chronic inflammation occurs in the pores mainly in the pilosebaceous system. It is defined as sex change, dandruff on the scalp
It is said that keratinized substances in the epidermis layer, sweat gland secretions and the like are entangled with each other and the secretions such as sebaceous glands are enhanced.

[0007] From the viewpoint of the cause of acne and dandruff in relation to the secretory function of sebum, triglyceride in sebum causes lipase by invasion of Propionibacterium acnes or Pitirosporum ovale into the hair follicle canal. The production of
The production of glycerol and free fatty acids is enhanced, and due to the excess free fatty acids produced at this time, a comedone forming action and an inflammation inducing action are particularly strongly observed.

Originally, both Propionibacterium acnes and Pitirosporum ovale are lipid-dependent and lipophilic, so that the amount of sebum increases and accumulates in the hair follicle tube, and thus Propionibacterium acnes. , Pitirosporum Obare also grows gradually. In addition, in addition to these, Propionibacterium acnes, neutrophil chemotaxis by Pitirosporum ovale and activator of complement, as well as protease, lipase, hyaluronidase, etc. of hair follicle epithelium produced by the bacterium. Destruction and induction of inflammation have also been proposed as new hypotheses.

Therefore, in the development of acne, it is important for the treatment of acne to prevent the growth of acne bacterium (Propionibacterium acnes) in the lesion area. Preventing the growth of the dandruff fungus (Pitirosporum ovale) in the lesion area leads to the treatment of dandruff and kayumi.

[0010] Therefore, various drugs have been used for the prevention and treatment of infectious diseases in vivo and in vivo, but most of them are antibiotics and synthetic antibacterial / preservatives.
Certainly, its effect is strong, but there is a problem in terms of safety, and strict caution is required for its use, and a more effective substance has been required in terms of its efficacy.

[0011]

Therefore, the inventors of the present invention have selected various substances that have antibacterial and antiseptic effects against infectious diseases and diseases caused by bacteria and are safe to use in various fields. Consider the ingredients obtained from the plant extract of
The pursuit has been accumulated.

[0012]

[Means for Solving the Problems] That is, in view of the above circumstances, the present inventors have confirmed that Artocarpin has a strong antibacterial / antiseptic effect against various bacteria, and The present invention was completed by discovering that it is very effective to apply to a new antibacterial / preservative and various cosmetics which are safe for animals. The process leading to the present invention will be described below.

The altocarpine of the present invention has the formula 1
It is a flavonoid compound with a structure that is mainly related to Artocarpus Gen
us) is a component contained in a genus plant, but the altocarpine of the present invention is not limited to those obtained from a specific plant, and those obtained by a chemical synthesis method can also be used. As for the refining / manufacturing method, any known refining / manufacturing method may be used.

[Formula 1] Formula 1

Further, altocarpine can be used as it is or in the state of using an organic solvent, water or hot water as a solvent, or if necessary dissolved in an organic solvent or a mixed solvent with water. It can also be used in a combination of solvent extraction and water extraction. As the organic solvent, methanol, ethanol, n-butanol, acetone, ethyl acetate, n-hexane, 1,3-butylene glycol, propylene glycol, diethyl ether, dimethyl ether, chloroform or the like can be used, and the form is a solution or paste. It can be used in the form of gel, gel or powder.

In most cases, altocarpine can be used as it is, but if necessary, purification treatment such as deodorization and decolorization may be added within a range that does not affect its efficacy. Further, as a purification treatment means for deodorization, decolorization, etc., an activated carbon column or the like may be used, and an ordinary means generally applied may be arbitrarily selected.

The altocarpine of the present invention can be used as it is, but the content in the case of being compounded as an antibacterial / preservative or cosmetic composition may vary depending on the kind of the antibacterial / preservative composition or the expected degree of action. different. Usually 2ppm
As described above, preferably 20 ppm or more.

The antibacterial / antiseptic agent or cosmetic containing the altocarpine of the present invention is generally used in foods, cosmetics, pharmaceuticals, quasi-drugs, etc. within a range not impairing the effects of the present invention. Various ingredients used, such as sugar, condensed milk, flour, shortening, salt, glucose, chicken eggs, butter,
Margarine, starch syrup, calcium, iron, seasonings, spices and oils (animal and vegetable oils, mineral oils, ester oils, wax oils,
Silicon oil, higher alcohols, phospholipids, fatty acids, etc., surfactants (anionic, cationic, amphoteric or nonionic surfactants), vitamins (vitamin A group,
Vitamin B group, folic acid, nicotinic acid, pantothenic acid, biotins, vitamin C group, vitamin D group, vitamin E group, other ferulic acid, γ-oryzanol, etc., ultraviolet absorber (p-aminobenzoic acid, anthranil, Salicylic acid, coumarin, benzotriazole, tetrazole, imidazoline, pyrimidine, dioxane, furan,
Pyrone, camphor, nucleic acid, allantoin and their derivatives, amino acid compounds, shikonin, baicalin, baicalein, berberine, etc., antioxidants (stearate, nordihydroguaselethenoic acid, dibutylhydroxytoluene, butylhydroxyanisole, para) Hydroxyanisole, propyl gallate, sesamol,
Sesamolin, gossypol, etc.), thickener (hydroxyethyl cellulose, ethyl cellulose, carboxyethyl cellulose, methyl cellulose, carboxymethyl cellulose, carboxymethyl cellulose sodium, hydroxypropyl cellulose, nitrocellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, Polyacrylate, carboxyvinyl polymer, gum arabic, gum tragacanth, agar, casein, dextrin, gelatin,
Pectin, starch, alginic acid and its salts), moisturizers (propylene glycol, 1,3-butylene glycol, polyethylene glycol, glycerin, chondroitin sulfate and its salts, hyaluronic acid and its salts, sodium lactate, etc.) Alcohol, polyhydric alcohol, water-soluble polymer, pH adjuster, antiseptic / antifungal agent, colorant, fragrance, cooling agent, stabilizer, animal / plant extract, animal / animal
Vegetable protein and its degradation product, animal / vegetable polysaccharide and its degradation product, animal / vegetable glycoprotein and its degradation product, microbial culture metabolic component, blood flow promoter, anti-inflammatory agent, anti-inflammatory agent, anti-allergic agent , A cell activating agent, an amino acid and its salt, a keratolytic agent, an astringent agent, a wound healing agent, a foaming agent, an oral agent, a deodorant / deodorant, an emulsifier and the like, and they can be used in combination.

The dosage form of the cosmetic composition containing the altocarpine of the present invention is arbitrary and may be prepared by a conventional method, for example, lotion, cream, ointment, lotion, shaving lotion, emulsion, cream (ointment). , Sunscreen, foundation, oil, pack, soap (including medicated soap), body soap,
Nail cosmetics, eyebrow cosmetics, perfumes, facial cleansers, oral products (toothpaste, mouthwash, etc.), liquid odors / deodorants, bath agents, shampoos, rinses, hairnics, hair sprays, hair dyes, etc. It can be a dosage form.

The dosage form of the antibacterial / antiseptic agent containing the altocarpine of the present invention is arbitrary and may be prepared by a conventional method. Cotton, wet tissues, wipes, non-woven fabrics such as sterilizing cloth, and oral compositions (gum, candy, etc.), fish paste products such as kamaboko, chikuwa, sausage, ham and other livestock products, Western confectionery,
Japanese confectionery, raw noodles, Chinese noodles, boiled noodles, noodles such as buckwheat, sauce, soy sauce, seasonings such as sauces, pickles, soy sauce, etc.,
It may be a general dosage form for food and drink.

Further, the form of the antibacterial / antiseptic agent and the cosmetics containing the altocarpine of the present invention may be any form, such as solution, cream, paste, gel, gel, foam, It can be used in solid or powder form.

The jackfruit extract containing altocarpine as an essential component of the same date, the kujin extract containing sophoraflavanone G as an essential component, and the like containing altocarpine or sophoraflavanone G. Any of these may be used, or they may be used in combination with antibacterial and antiseptic agents and cosmetics.

[0022]

EXAMPLES The present invention will be described in more detail with reference to the following examples, but the method described below is used in the confirmation test of the action and the like described later, and is not limited to this.

Example 1: Confirmation of altocarpine from jackfruit

1 kg of jackfruit wood was crushed, 10 L of acetone was added, and the mixture was heated under reflux at about 75 ° C. for extraction for 3 hours, cooled, filtered, and then subjected to solid / liquid separation, and the separated extract was decompressed. After concentration and removal of acetone, the concentrated and concentrated extract was passed through silica gel column chromatography (manufactured by Merck & Co., Inc.) and sequentially eluted with each of the following extraction solvents (benzene, chloroform, ethyl acetate, acetone, methanol), Obtain the eluate. When the obtained elution fractions were screened for antibacterial activity, a strong activity was observed in the chloroform elution fraction. Therefore, when a component search was performed on this eluted fraction, it was confirmed that altocarpine was contained as a main component, and as a result of conducting an antibacterial test again on altocarpine, a strong antibacterial effect was found.

Example 2: Purification of altocarpine About 50 g of jackfruit wood was crushed, 500 ml of acetone was added, and the mixture was heated under reflux extraction at about 75 ° C. for 3 hours, cooled, filtered, and then solid-liquid separation was performed. The separated extract is concentrated under reduced pressure to remove acetone, and then the concentrated extract is eluted with silica gel column chromatography (Merck & Co., Inc.) benzene: ethyl acetate = 10: 1 to obtain an eluate. Then, the eluate is recrystallized to obtain about 86 mg of altocarpine.

[0026] Example 3: Staphylococcus aureus, the growth inhibition test against B. subtilis [test bacteria] 1. Staphylococcus aureus Staphylococcus aureus: (ATCC6538) 2. Bacillus subtilis Bacillus subtilis: (ATCC93
72) [Used medium] Brain Heart Infusion Broth "Eiken" (BHI) 3
Dissolve 7 g in 1000 ml of purified water, and sterilize under pressure at 121 ° C. for 15 minutes. [Preparation of Sample Bacterial Fluid] To 10 ml of the above medium, 1 platinum loop of the test bacterium was added, and the mixture was incubated at 37 ° C.
After culturing for a period of time and diluting this with the same medium to a viable cell count of about 10 ⁶ , it is used as a sample bacterial solution. [Preparation of Specimen] 1 mg of altocarpine was dissolved in 1 ml of dimethyl sulfoxide (hereinafter, DMSO) to prepare a specimen solution, and methyl paraoxybenzoate was similarly subjected. [Test Method] After adding 0.1 ml of the sample solution and 0.1 ml of the sample bacterial solution to 1.8 ml of BHI medium and anaerobically culturing at 37 ° C. for 24 hours (manufactured by Gas Pack BBL), agar plates (manufactured by Daigo Nutrition) were added. After streaking and confirming the remaining bacteria, the minimum inhibitory concentration (ppm) was determined by the multiple dilution method. The results are shown in Table 1.

[0027]

[Table 1]

[0028] [Test Results] Alto pilocarpine is minimum growth inhibitory concentration against Staphylococcus aureus, Bacillus subtilis, 1 ppm and can be determined, was confirmed to have a growth inhibitory effect in very small quantities. It was confirmed that it has a very strong growth inhibitory effect as compared with methyl paraoxybenzoate used as a control.

[0029] Example 4: Growth inhibition test against Propionibacterium acnes [Preparation of test strains] Propionibacterium acnes (Propionibacter
ium acnes (hereinafter referred to as "PA bacterium") is a suspension (2.0 x 1) of the strain identified and isolated from the total amount of healthy volunteers in our laboratory.
( ⁹ cells / ml), the strain stored frozen was used and diluted 10-fold with a liquid medium to give a test bacterial solution. [Liquid medium] * 1 Nutrient broth (Difco) 0.5 g Yeast extract 0.3 g Tween 80 0.2 ml Purified water 100 ml [Preparation of sample] 1 mg of altocarpine is dissolved in 1 ml of DMSO to prepare a sample solution. [Test method] To 1.8 ml of liquid medium, 0.1 ml of the sample solution and 0.1 ml of the test bacterial solution were added, followed by anaerobic culture at 37 ° C. for 7 days (Gas
Pack BBL), 10 μl of the culture solution was streaked on GAM agar medium (Nissui Pharmaceutical Co., Ltd.), and after anaerobic culture at 37 ° C. for 48 hours, residual bacteria were confirmed, and the minimum inhibitory concentration (ppm) was determined by the multiple dilution method. ) Judged. The results are shown in Table 2.

[0030]

[Table 2]

The minimum growth inhibitory concentration for PA bacteria [Test Results] Alto pilocarpine is
Although it could be determined to be 19.5 ppm, it was also confirmed that the growth inhibitory effect was obtained even at 2.4 ppm. Therefore, it was confirmed that altocarpine had a growth inhibitory effect on PA bacteria even in a very small amount.

[0032] Example 5: Pitirosuporumu growth inhibition test against Obare bacteria [test strains] Pitirosuporumu-Obare fungus (Pityrosporum ovale,
(PO bacteria) is a strain that was isolated and identified from the total amount of healthy volunteers in our laboratory and frozen and stored as a suspension (1.6 x 10 ⁶ cells / ml). Was diluted to give a test bacterial solution. [Liquid medium] * 2 Glucose 2 g Polyoxyethyleneglycerin monooleate (5EO) 5 ml Potato extract 100 ml [Preparation of sample] 1 mg of altocarpine is dissolved in 1 ml of DMSO to prepare a sample solution. [Test Method] To 1.8 ml of liquid medium, 0.1 ml of the sample solution and 0.1 ml of the test bacterial solution were added, and after anaerobic culture at 30 ° C. for 2 days (Gas
(Pak BBL), 10 μl of the culture solution was streaked on a potato dextro-agar medium (manufactured by Nissui Pharmaceutical Co., Ltd.) containing 5% polyoxyethylene glycerin monooleate (5EO), and anaerobically cultured at 30 ° C. for 24 hours. After that, the remaining bacteria were confirmed, and the minimum inhibitory concentration (ppm) was determined by the multiple dilution method. The results are shown in Table 3.

[0033]

[Table 3]

[0034] [Test Results] minimum growth inhibitory concentration for PO bacteria Alto pilocarpine is
Although it can be determined to be 2.0 ppm, it was confirmed that altocarpine has a growth inhibitory effect at a lower concentration.
It was confirmed that altocarpine has a growth inhibitory effect on PO bacteria even in a very small amount.

[0035] Example 6: For acne and skin roughness improvement test "test method and the evaluation method" in Table 4 below to the product of the present invention and comparative product of cream shown, as a panelist adult men and women 20 people acne disease, night and morning every day 2 times, take an appropriate amount and apply it on your face,
After that, a continuous use effect test was freely conducted for two months. The evaluation method was carried out according to the following criteria, and the results are shown in Table 5 and the numerical values in the table represent the number of people. "Acne improvement effect" Effective: Acne was improved. Slightly effective: The acne was slightly improved. Ineffective: No change from before use. "Rough skin improvement effect" Effective: Rough skin was improved. Slightly effective: Rough skin was slightly improved. Ineffective: No change from before use.

[0036]

[Table 4]

[0037]

[Table 5]

[0038] [Test Results] The results are as shown in Table 5, cream formulated with alto Karpin of the present invention product 1-2, acne is improved, also, good results as compared with the comparative product also rough skin It was

[0039] Example 7: antidandruff and itching improved test

[ Test Method and Evaluation Method ] With respect to the shampoos of the present invention product and the comparative product shown in Table 6 below, 20 adult men and women with dandruff or itchiness associated with dandruff were used as panelists every day at night for bathing. Each time, an appropriate amount was picked up, and the hair was washed. After that, the continuous use effect test was conducted freely for 2 months. Similarly, the following Table 7
Regarding the hair tonic of the present invention product and the comparative product shown in
As panelists, 20 adult men and women with dandruff or itchy acne associated with dandruff, picked up an appropriate amount twice daily in the morning and at night, had it applied to the hair, and then freely for 2 months. A continuous use effect test was conducted. The evaluation method was carried out according to the following criteria. The results are shown in Table 8 and the numerical values in the table represent the number of people. "Dandruff control effect" Effective: The dander was suppressed. Slightly effective: Dandruff was slightly suppressed. Ineffective: No change from before use. "Kayumi improvement effect" Effective: Kayumi was improved. Slightly effective: Kayumi improved slightly. Ineffective: No change from before use.

[0041]

[Table 6]

[0042]

[Table 7]

[0043]

[Table 8]

[0044] [Test Results] The results are as shown in Table 8, the present invention product 1-2 shampoo or hair tonic formulated with Alto pilocarpine is antidandruff and itching improved, as compared with the comparative product, very Very good results were obtained.

[0045] Example 8: Safety test solution prepared by dissolving powder 10mg of [primary skin irritation test] Alto pilocarpine to 50% 1,3-butylene glycol aqueous solution 10 ml, also Alto pilocarpine powder 10mg of 60% aqueous ethanol solution 10m
Rabbits with hair removed from the solution (1 group 3 animals, body weight 3,
About 800 g) was applied to the skin. Judgment is 24, 48, 72 after attachment
Erythema and edema were used as indices by the scoring method of primary irritation during time. As a result, in all animals,
Neither erythema nor edema was observed and the result was negative. [Skin cumulative irritation test] Similarly, 10 mg of altocarpine powder was added to 50% 1,3
A liquid dissolved in 10 ml of an aqueous solution of butylene glycol,
Also, a solution of 10 mg of altocarpine powder dissolved in 10 ml of 60% aqueous ethanol solution was shaved on the flank (2 x 4 cm2).
Hartley guinea pigs (female, 5 per group, weight 32)
(About 0 g) was applied to the skin once a day, 5 times a week, 0.5 ml / animal. The application was carried out for 4 weeks, and the hair removal was performed on the last application day of each week. Judgment was performed on the day after the last day of each week, using erythema and edema as an index by the primary irritation scoring method. As a result, in all animals, erythema and edema were not observed for 1 to 4 weeks after application, and it was determined to be negative. [Acute toxicity test] Similarly, 2,000 mg / kg to ddy mice (5 mice per group, body weight 30 g) fasted for 4 hours with altocarpine powder before the test.
The amount was orally administered, and the onset and degree of toxicity were observed over time. As a result, no abnormality was observed in all mice for 14 days, and there was no abnormality in the autopsy result. LD50 is 2,
It was determined to be 000 mg / kg or more.

[0046] the following examples, but further detailing the usage of the present invention, the present invention is not specified in the examples below, various foods, cosmetics, pharmaceuticals, quasi-drugs Etc. can be used by being contained and compounded. In addition, each Example may be manufactured by a conventional method for manufacturing each product, and only the compounding amount is shown.

[0047] Example 9: cold cream Weight% 1. White beeswax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Almond oil 15.0 5. Borax 0.5 6. Altocalpine 0.4 7. Suitable amount of perfume 8. Residue of purified water 100

[0048] Example 10: Lotion Weight% 1. Sorbit 2.0 2.1,3-butylene glycol 2.0 3. Polyethylene glycol 1000 1.0 4. Polyoxyethylene oleyl ether (25E.O.) 2.0 5. Ethanol 10.0 6. Altocalpine 1.0 7. Suitable amount of perfume 8. Residue of purified water 100

[0049] Example 11: Bread Weight% 1. Flour 94.0 2. Salt 0.9 3. Glucose 5.0 4. Altocalpin 0.1

[0050] Example 12: sausage Weight% 1. Ground meat 94.0 2. Chicken egg 5.0 3. Spice 0.3 4. Seasoning 0.5 5. Altocarpin 0.2

[0051] Example 13: fruit juice drinks Weight% 1. Glucose liquid sugar 33.0 2. Grapefruit juice 65.0 3. Fragrance 1.0 4. Altocalpine 1.0 5. Acidulant Suitable amount

[0052] Example 14: Gum Weight% 1. Menthol Micron 32.0 2. Grapefruit flavor 65.0 3. Altocalpin 3.0

[0053]

INDUSTRIAL APPLICABILITY The altocarpine of the present invention has a growth inhibitory action against various bacteria (Staphylococcus aureus, Bacillus subtilis, acne bacterium, dandruff bacterium) and is safe for humans or animals. . Therefore, when it is used as an antibacterial / antiseptic agent, it can prevent spoilage / contamination of various forms of preparations / products. Therefore, various forms of oral drugs, textile products (sheets, clothes), sanitary cotton, sanitization, etc. It can be applied to non-woven fabrics such as business cloth, and to general food and drink. Further, when used as a cosmetic, it has the effects of improving acne and rough skin, and suppressing dandruff and itchiness. Therefore, it also includes pharmaceuticals, quasi-drugs, and cosmetics (including various preparations used for humans and other animals. )
It can be applied to various cosmetics. Furthermore, it is considered to have an antibacterial action against oral fungi such as cariogenic bacteria and can be used for the prevention of symptoms such as tooth decay and periodontal disease.

Continuation of the front page (51) Int.Cl. ⁷ Identification code FI A61K 7/00 A61K 7/00 Y 7/06 7/06 7/48 7/48 31/352 31/352 A61P 31/04 A61P 31/04 (56) References JP-A-6-157267 (JP, A) JP-A-63-141907 (JP, A) K.K. M. Renuka Prasad et al, PURICATION AND CHRACTERIZATIO N OF A PROTEASEFROM M JACKFRUIT LATEX, Phytochemistry, 1999, Vol. 29, No. 6, pp. 1763-1766 Phytotherapy Research arch, 1990, Vol. 4, No. 6, pp. 235-236 (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 35/78 A61K 7/00 BIOSIS (DIALOG) CA (STN) MEDLINE (STN)

Claims (4)

(57) [Claims] 1. An antibacterial / antiseptic agent containing altocarpine. 2. The antibacterial and antiseptic agent according to claim 1, which has a growth inhibitory action against Staphylococcus aureus, Bacillus subtilis, Acne and Dandruff. 3. A skin cosmetic, which comprises altocarpine. 4. A hair cosmetic composition containing altocarpine.